Académique Documents
Professionnel Documents
Culture Documents
Viscosity
Understanding effects of viscosity in the BioFlux system
Introduction
In the BioFlux software, the user inputs the desired shear stress under which to run the experiment. From this input value, there are equations used to populate the other fields visible to the user. These equations calculate values for shear stress, volumetric flow rate, run time and pressure. In order to apply the correct amount of air pressure to the plate, and thus apply the correct shear, the equations must factor in the correct viscosity of the fluid. The viscosity of a fluid describes its resistance to flow. In the BioFlux software, this value is entered in the Fluid Properties manager. A number of commonly used fluids are preset in the software, such as water and PBS. Custom fluids (and their viscosities) can also be entered in the Fluid Properties module. This technical note will describe some of the important aspects of viscosity and serve as a guide for planning experiments in the BioFlux system.
1.4 1.2
Viscosity (c P)
Figure 1: A plot of viscosity versus temperature for water. Viscosity change is approximately linear from 10-40C but the slope changes when the temperature rises above 40C.
Viscosity (cP)
Figure 2: Viscosity was measured for 6 commonly used fluids at room temperature and 37C. Viscosity drops with an increase in temperature for all fluids.
1038-01 1/27/09
Viscosity
Understanding effects of viscosity in the BioFlux system
TECHNICAL NOTE
22
23
1.20
1.17
D-Phosphate buffered saline CaMg free (UCSF CCFAL003) HBSS with calcium and magnesium, without phenol red (Hanks BSS) (UCSF CCFAJ002) M63 Minimal Salts (Pardee et al 1959 + 1mM sodium citrate+ 1mM MgSO4) Luria Broth - Teknova L8050 TSB TBD BBL Trypticase soy broth (ref #299113) Williams complete+10%FBS (Invitrogen cat #12551-032)
Further information
Kameneva MV (1999) Gender difference in rheologic properties of blood and risk of cardiovascular diseases. Clin Hemorheol Microcirc. 21(3-4):357-63. Viscosities for other common fluids can be found at: A http://www.engineersedge.com/fluid_flow/fluid_data.htm http://www.cvphysiology.com/Hemodynamics/H011.htm, http://en.wikipedia.org/wiki/Viscosity
2008 Fluxion Biosciences, Inc. All rights reserved."BioFlux", "CytoFlux" and "IonFlux" are trademarks of Fluxion Biosciences, Inc.
www.fluxionbio.com