International Journal of Pharmacy and Pharmaceutical Sciences

ISSN- 0975-1491 Vol 3, Issue 2, 2011

ResearchArticle

DEVELOPMENTOFUVSPECTROPHOTOMETRICMETHODFORESTIMATIONOFMEFENAMIC ACIDINBULKANDPHARMACEUTICALDOSAGEFORMS
HARINDERSINGH*,RAJNISHKUMAR,PINDERJITSINGH
StateFood,DrugandExciseLaboratory,Punjab,Sector11D,Chandigarh,India,(DepartmentofHealthandFamilyWelfare,Punjab) Email:harindersingh.pharm@gmail.com Received:12Jan2011,RevisedandAccepted:16Feb2011 ABSTRACT A simple, sensitive and specific UV spectrophotometric method was developed for the estimation of Mefenamic acid in tablet dosage form. The optimumconditionsfortheanalysisofthedrugwereestablished.Thewavelengthmaxima(max)forMefenamicacidwerefoundtobe285nm. Beerslawwasobeyedintheconcentrationrangeof560mcgmL1with10.2799x104Lmol1cm1,theslope,intercept,correlationcoefficient, detectionandquantizationlimitswerealsocalculated.Theproposedmethodhasbeenappliedsuccessfullyfortheanalysisofthedruginpureand initstabletsdosageforms. Keywords:Mefenamicacid,UVspectrophotometer INTRODUCTION Mefenamic acid (MFNC) is 2[(2,3dimethylphenyl) amino] benzoic acid and is used as an analgesic and antiinflammatory agent1. The drugisofficialinBritishPharmacopoeiawithestimationofthedrug bynonaqueoustitrimetricmethod2.Literaturesurveyrevealsthat spectrophotometric, HPLC and LC37 methods have been reported fortheestimationofMFNCfrompharmaceuticalformulations.But to the best of our knowledge, there is no work in the literature reportedabouttheUV spectrophotometricmethod forthe analysis of MFNC in pharmaceuticals. Hence, the authors have made an attempt to develop a simple and rapid UV spectrophotometric method for the estimation of MFNC in the bulk and in pharmaceutical formulations. Developed spectrophotometric methodswerefoundtobesimple,rapid,accurate,reproducibleand economicalincomparisontoreportedmethodsusedforanalysis of singledrug. MATERIALANDMETHODS Instrumentandapparatus PerkinElmer UVVisibleSpectrophotometerLambda25modelwas used for spectral measurements with spectral band width 1 nm,

wavelength accuracy is 0.5 nm and 1 cm matched quartz cells. Glassware used in each procedure were soaked overnight in a mixture of chromic acid and sulphuric acid rinsed thoroughly with doubledistilledwateranddriedinhotairoven. Reagentsandmaterials All chemicals and solvents were of analytical reagent grade and doubledistilledwaterwasusedtopreparesolutions. Standarddrugsolution Pharmaceutical grade MFNC was kindly provided by Cipla Ltd., India. A stock standard solution equivalent to 1mg/mL MFNC was preparedbydissolving50mgofpuredrugin0.1MHClanddiluting to50mLincalibratedflaskwith0.1MHCl. Method Different aliquots (0.0, 0.5, 1.0, , 7.0 mL) of 1 mg/mL MFNC solutionwereaccuratelymeasuredandtransferredintoaseriesof 100 mL volumetric flasks and volume made up to the mark with 0.1 M HCl. Then all dilutions were scanned between 200400 nm against blank which shows the maximum absorbance at 285 nm (Fig.1).

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0. A 0.

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200. 21 22 23 24 25 26 27 28 29 30 n 31 32 33 34 35 36 37 38 39 400.0

Fig.1:UVspectraofMFNC Thesamemaxwasusedforfurthermeasurementofdrug.Acalibrationcurveforabsorbancevs.concentrationwasplotted(Fig.2).

Singhetal. IntJPharmPharmSci,Vol3,Issue2,2011,237238

Calibration curve of Mefenamic Acid

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A b so rb a n ce

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y=0.0426x+0.0038 correlation coefficient=0.9999

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0.0 0 10 20 30 40 50 60

Concentration(ppm)

Fig.2:StandardplotforMFNC(Absorbanceat285nm) Assayofpharmaceuticalformulations Twenty tablets were weighed accurately and ground into a fine powder. Powder equivalent to 100mg of MFNC was weighed accurately and transferred into a 100 mL volumetric flask with 60 mL 0.1 M HCl. The content was shaken for 1520 min, diluted to volume with 0.1 M HCl, and filtered using a Whatman No. 42 filter paper.First10mLportionoffiltratewasdiscardedandsubsequent portionsweresubjectedtoanalysis. RESULTSANDDISCUSSION TheabsorptionspectrumofMFNCwasmeasuredintherange200 400nmagainsttheblanksolution0.1MHClsimilarlyprepared.The standard solution show maximum absorbance at max for each threesystemsasrecordedinTable1.Andthemethodwasvalidated bystudyingthefollowingparameters

Table1:ParametersfordeterminationofMFNCagainstblank Parameters max,nm Beerslawlimit,gmL1 Molarabsorptivity,Lmol1cm1 Regressionequation Slope(m) Intercept(c) Correlationcoefficient

Values 285 560 10.2799x104 0.0426 0.0038 0.9999

The accuracy of the above method was ascertained by comparing theresultsobtainedwiththeproposedandreferencemethodsinthe caseofformulationarepresentedinTable2.

Table2:AssayandrecoveryofMFNCinpharmaceuticaldosageforms Formulation Labelclaim(mg) Amountfound(mg) %Recoveryproposedmethod# F1 100 99.67 99.67 F2 100 99.73 99.73 F1andF2aretabletsfromdifferentbatches(MeftalP,BlueCrossLaboratoriesltd,India) *Referencemethod3. #Recoveryamountwastheaverageofsixdeterminants.

%Recoveryreferencemethod* 99.09 98.83

As an additional check on the accuracy of these methods, recovery experiments were performed by adding known amounts of pure drugtopreanalyzedformulationandpercentrecoveryexperiments were also done. Recovery experiments indicated the absence of interferences from the commonly encountered pharmaceutical additivesandexcipients. CONCLUSION Itcouldbeconcludedthatthedevelopedmethodforestimationof MFNC in pharmaceutical dosage forms and in bulk is simple, sensitive, relatively precise and economical. The proposed methods are used for the routine analysis of the drugs in the qualitycontrol. ACKNOWLEDGEMENTS The authors are grateful to the Mr. Pankaj Sareen (Government AnalystPunjab),Mr.BalwinderSinghandMr.AshokGargesh(Public Analyst,Punjab)forprovidingtheircontinuoussupportthroughout thework.AuthorsarealsogratefultoCiplaLtd.,Indiaforproviding thegiftsampleofMFNC.

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