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Ann. occup. Hyg., Vol. 47, No. 3, pp.

219–226, 2003
© 2003 British Occupational Hygiene Society
Published by Oxford University Press
DOI: 10.1093/annhyg/meg024

Exposure Assessment to α- and β-Pinene, ∆3-Carene


and Wood Dust in Industrial Production of Wood
Pellets
K. EDMAN1*, H. LÖFSTEDT1, P. BERG1, K. ERIKSSON2, S. AXELSSON1,
I. BRYNGELSSON1 and C. FEDELI1,3

1Department of Occupational and Environmental Medicine, Örebro University Hospital, SE-701 85


Örebro, Sweden; 2Department of Occupational and Environmental Medicine, University Hospital of
Umeå, SE-901 85 Umeå, Sweden; 3Department of Business Administration, Computer Science,
Economics and Statistics, Örebro University, SE-701 82 Örebro, Sweden

Received 15 July 2002; in final form 7 October 2002

The main aim of the study was to measure the exposure to monoterpenes (α- and β-pinene and
∆3-carene) and wood dust during industrial production of wood pellets and briquettes.
Additional aims were to compare the results from wood dust sampled on a filter with real time
measurements using a direct reading instrument and to identify peak exposures to dust.
Twenty-four men working at six companies involved in industrial production of wood pellets
and briquettes participated in the study. Monoterpenes were measured by diffusive sampling
and wood dust was measured as total dust. A data logger (DataRAM) was used for continuous
monitoring of dust concentration for 18 of the participants. The sampling time was ∼8 h. The
personal exposure to monoterpenes ranged from 0.64 to 28 mg/m3 and a statistically significant
(Kruskal–Wallis test, P = 0.0002) difference in levels of monoterpenes for workers at different
companies was seen. In the companies the personal exposure to wood dust varied between 0.16
and 19 mg/m3 and for 10 participants the levels exceeded the present Swedish occupational
exposure limit (OEL) of 2 mg/m3. The levels of wood dust during the morning shift were signifi-
cantly (Mann–Whitney test, P = 0.04) higher compared with the afternoon shift. Continuous
registration of dust concentration showed peak values for several working operations,
especially cleaning of truck engines with compressed air. For 24 workers in six companies
involved in industrial production of wood pellets the personal exposure to monoterpenes was
low and to wood dust high compared with the present Swedish OEL and previous studies in
Swedish wood industries. Since the DataRAM can identify critical working tasks with high
wood dust exposure a reduction in exposure levels could probably be achieved by changes in
working routines and by the use of protective equipment.

Keywords: DataRAM; monoterpenes; total dust; wood dust; wood pellets

INTRODUCTION produced in 2000. Wood pellets and briquettes are


produced from compressed wood shavings and
The environmental and energy policy in Sweden
sawdust from pine (Pinus sylvestris) and spruce
aims at replacing fossil energy with renewable
(Picea abies). Pine and spruce contain monoterpenes,
sources such as biofuels, of which wood pellets and
with α-pinene, β-pinene and ∆3-carene as the main
wood briquettes are examples. The production of
wood pellets in Sweden has increased by 50% during constituents (Fengel and Wegener, 1983). These
the last 5 years and around 700000 tons were substances are released not only during mechanical
treatment of the wood but also during storage of
sawdust and shavings.
Monoterpenes are irritating to the skin, eyes and
*Author to whom correspondence should be addressed. mucous membranes and can cause both non-allergic
E-mail: katja.edman@orebroll.se and allergic contact dermatitis (Eriksson and Levin,

219
220 K. Edman et al.

1990; Falk-Filipsson, 1995). Monoterpenes can also amount of airborne particles that can be inhaled
easily penetrate the different barriers of the body and through the mouth and nose (Eriksson and Liljelind,
uptake of pinenes can occur through the lungs, the 2000; ACGIH, 2002). Total dust is defined as dust
gastrointestinal tract and intact skin (Cavender, measured on a filter in an open-faced cassette with a
1994). The occupational exposure limit (OEL) in diameter of 37 or 25 mm.
Sweden is 150 mg/m3 for either individual mono- In general, exposure to wood dust is expressed as
terpenes or their sum (Arberskyddsstyrelsen, 2000). the average air level during the sampling time. This
Air levels between 10 and 550 mg/m3 have been sampling technique gives limited information about
measured in previous studies in sawmills and joinery the variations of exposure over time. Instruments that
shops in Sweden (Hedenstierna et al., 1983; Eriksson provide real time data are therefore useful to identify
and Levin, 1990; Eriksson et al., 1996, 1997). In a emission sources and work tasks which may cause
study previously done in sawmills in Finland a high short-term exposures. As the production of
geometric mean (GM) between 2.0 and 138 mg/m3 wood pellets and briquettes is increasing, it is import-
was reported (Rosenberg et al., 2002). In Canada ant to study the occupational exposure in order to
Demers et al. (2000) have shown a GM for mono- prevent health effects in the future.
terpenes of 0.5 mg/m3 within a large lumber mill The main aim of this study was to measure the
handling pine, spruce and fir. exposure to monoterpenes (α- and β-pinene and ∆3-
In addition to monoterpenes, the employees are carene) and wood dust during industrial production
exposed to wood dust. Wood dust from pine and of wood pellets and briquettes. Additional aims were
spruce has been reported to cause irritation in the to compare the results from wood dust sampled on a
eyes and upper airways at air levels between 0.1 and filter with real time measurements using a direct
6.3 mg/m3. There are also indications that wood dust reading instrument and to identify peak exposures to
levels around 1 mg/m3 may cause reduced lung func- dust.
tion (Eriksson and Liljelind, 2000). Moreover, expo-
sure to wood dust from these species can cause
eczema (Färm, 1997). Air levels between 0.1 and 7.3 MATERIALS AND METHODS
mg/m3 have been measured in the Swedish wood-
work industry (Hedenstierna et al., 1983; Eriksson et Company data
al., 1996, 1997; Eriksson and Liljelind, 2000). Many Six companies located in central Sweden partici-
measurements of wood dust from soft woods (pine pated in the study. The companies were located near
and spruce) have been performed in sawmills in our research group and were chosen on the basis of
Finland, Germany, Denmark and Canada and in location. They all produced wood pellets and two
carpentries in Finland, Denmark, Germany, the UK, of the companies also manufactured briquettes. The
USA and Canada. The mean air levels in sawmills production of wood pellets at each company ranged
were between 0.1 and 7.8 mg/m3 and in the carpen- from 12000 to 40000 tons/yr. In the production
tries between 0.8 and 18 mg/m3 (IARC, 1995). process of wood pellets, sawdust is always dried
The present Swedish OEL for wood dust measured before grinding, while shavings can be ground at
as total dust is 2 mg/m3, but the Swedish Labour once. After grinding, the material is pressed in the
Protectorate recommends an exposure <1 mg/m3 presses at 100°C (Fig. 1). The wood pellets are then
when new enterprises are established or old fac- transported through a cooling tower to bagging or
tories are rebuilt (Arberskyddsstyrelsen, 2000). The storage or for transportation via trucks. The produc-
American Conference of Governmental Industrial tion of briquettes uses a simpler technique, where
Hygienists (ACGIH) recommended OEL for soft- sawdust and shavings are mechanically forced
woods is 5 mg/m3 measured as inhalable dust, but together. The participants worked morning, afternoon
with a notice of intended change to 2 mg/m3 for ‘non- or day shifts on the day of measurement. However,
allergenic and noncarcinogenic’ wood dusts and to not all companies used a day shift (Table 1). All com-
1 mg/m3 for ‘other respiratory allergic wood dusts’ panies had general ventilation and some companies
(ACGIH, 2002). Inhalable dust is defined as the total had local exhaust ventilation situated at specific

Fig. 1. Schematic of the production process for wood pellets.


Production of wood pellets 221

Table 1. Number of employees, participants and working shifts for personal measurements and test sites for the static
measurements at each company
Company Employees (n) Personal measurement Static
measurement
Participants (n) Working shifts (n) Test sites (n)
Morning Afternoon Day

1 9 7 2 2 3 3
2 4 2 1 1 0 4
3 2 2 1 1 0 5
4 5 5 2 2 1 3
5 13 6 2 2 2 5
6 6 2 1 1 0 5
Total 39 24 9 9 6 25

worksites, for example at bagging and briquette pro- Analysis of monoterpenes


duction. Desorption of monoterpenes was performed with
an automatic thermal desorber (Perkin Elmer 400)
Study population
connected to a gas chromatograph (GC) (Hewlett
Thirty-nine workers were employed in the indus- Packard 6890) with a mass selective detector
trial production of wood pellets in the six companies. (Hewlett Packard 5972). The samples were desorbed
The 24 men who were present at work on the day of at 200°C for 5 min at a desorption flow of 70 ml/min
measurement were asked to participate and all helium (He) without an inlet split and collected on a
accepted. Working operations for morning and after- Tenax TA filled trap at –30°C. The analytes were
noon workers are, among others: loading of raw desorbed from the trap at 200°C for 10 min with an
material, monitoring from the control room, main- outlet split flow of 50 ml/min. The column used was
tenance work, repairs, sweeping and cleaning of a 50 m × 0.32 mm capillary with a film thickness of
truck engines with compressed air. Day shift workers
1.05 µm of crosslinked methylsiloxane (HP-1). The
mainly drove trucks and worked on bagging. The
temperature of the GC was programmed as follows:
protective equipment was hearing protectors and
initial 50°C hold for 1 min, 5°C/min to 120°C,
respirators. The respirators were seldom used during
35°C/min to 290°C and hold for 10 min. For identifi-
the measurements.
cation of the monoterpenes the mass selective
Sampling strategy detector was run in the full scan mode (29–550
a.m.u.) after a solvent delay of 8 min. The monoter-
In all companies measurements were made during
penes were identified against a NIST/EPA/NIH mass
an afternoon shift (14.00–22.00) and during the
spectral database (HP G1033A revision C.00.00
following morning shift (6.00–14.00). In three of the
six companies measurements were also carried out 1992). The total ion chromatogram signal of the
during the day shift (7.00–16.00) on the same day as monoterpenes was used for quantification. The
the measurements for the morning shift. The monoterpenes were quantified against calibration
sampling time was ∼8 h. graphs ranging from 50 ng to 2 µg/sample. The
calibration points were prepared by injection of 3 µl
Monoterpenes of a standard solution of monoterpenes in methanol
Air sampling of monoterpenes was done by diffu- onto a Tenax TA tube under a flow of 100 ml/min He
sive sampling using a Perkin Elmer tube with Chro- over 1 min and analysed in the same way as the field
mosorb 106 (Markes International) as adsorbent samples. The limit of detection (LOD) was estimated
(Sunesson et al., 1999). The sampler was attached to to be 7 ng and was calculated as 3 SD of the signal at
the lapel of the workers overalls, i.e. in the breathing the lowest calibrated concentration. The coefficient
zone. Static samples were taken at three to five of variation was 5% at the 50 ng/sample level (n =
different positions in each company (Table 1). The 12). The highest concentration on the calibration
samplers were positioned at strategic points where curve was 2 µg/sample for the different mono-
high wood dust and/or monoterpene exposure was terpenes, but previous experience suggests that the
expected and the participants worked, such as the curve is linear at higher concentrations, therefore
sawdust and shaving store, or where the workers extrapolation was done for higher concentrations
spent a lot of time, for example the control room. (K. Eriksson, University Hospital of Umeå).
222 K. Edman et al.

Wood dust monoterpenes, as well as between wood dust on


Pumped sampling of wood dust was done using filters and wood dust analysed with a DataRAM, the
a 25 mm cellulose acetate filter placed in an open- Spearman correlation was used. Differences in
faced, antistatic cassette with an air flow of 2 l/min. personal exposure between companies were tested
The filter cassette was placed in the breathing zone with the Kruskal–Wallis non-parametric test with a
by attaching it to the collar of the workers shirt or Monte Carlo estimate of the exact P-value, since the
overalls. The filters were conditioned for 48 h at 20 ± number of observations was low and the distribution
1°C and at a relative humidity of 50 ± 3% before and of the variable was skewed. For the same reason, the
after the sampling. The filters were gravimetrically differences in exposure between morning and after-
determined using a scale with a lowest detection level noon shifts were analysed with the Mann–Whitney
of 0.001 mg. Static sampling was performed at the test with an exact P-value. Measurements under the
same sample sites as for the monoterpenes. LOD were recorded as LOD/√2 (Hornung and Reed,
1990). If at least half of the values in one group were
Real time data for wood dust under the detection limit there was no calculation
Continuous monitoring of dust concentration was done for GM and AM.
done using a personal data logging, real time aerosol
monitor (DataRAM; MIE, Bedford USA) in parallel RESULTS
with collecting wood dust on a filter for 18 of the
participants. The DataRAM is a photometric monitor Air concentration of monoterpenes
and measures particles with a diameter between 0.1 In total, 24 exposure measurements of mono-
and 10 µm in the range 0.001–400 mg/m3. The terpenes were performed and the GM of the personal
DataRAM relies on the diffusion of ambient air into a exposure to monoterpenes for each of the six com-
sensing chamber and the optimal sensitivity is, panies varied from 1.8 to 17 mg/m3 (Table 2). All the
according to the manufacturer, for the respirable frac- full-shift measurements ranged from 0.64 to 28 mg/m3.
tion of dust (<5 µm). The DataRAM was calibrated High levels of monoterpenes were noticed at com-
against SAE Fine (ISO Fine; Powder Technology), a pany number 4 compared with the other companies
test dust, by the manufacturer. The DataRAM was and a statistically significant (P = 0.0002) difference
placed on the belt of the worker and the intake of air in monoterpene levels for workers at different com-
was from below. Registration of the dust concentra- panies was seen. No significant (P = 0.99) difference
tions was done every 20 s and stored in a data logger in levels of monoterpenes was seen between workers
in the instrument and then transferred to a PC. A peak on the morning shift compared with the afternoon
was defined as successive registrations over a shift. The air levels obtained by static sampling
threshold value of 0.4 mg/m3. During the DataRAM ranged from <0.84 to 74 mg/m3 (Table 3). Low levels
measurements the participants were asked to keep a of monoterpenes were measured at the grinder (GM
work diary and to register time as well as duration of 1.2 mg/m3) and high levels in the sawdust and
different working tasks. shaving store (GM 12 mg/m3).

Statistical analysis Wood dust on filter


The descriptive measures calculated for concentra- For all companies the personal exposure to wood
tions of wood dust and monoterpenes are the GM and dust varied between 0.16 and 19 mg/m3, with a GM
arithmetic mean (AM), along with the range. For for each company between 0.63 and 19 mg/m3 (Table
analysis of correlations between wood dust and 2). One of the measurements (3.7 mg/m3) was under-

Table 2. The personal exposure to monoterpenes (sum of α- pinene, β-pinene and ∆3-carene) and wood dust at the six companies
Company Participants (n) Total monoterpenes (mg/m3) Wood dust (mg/m3)
GM AM Range GM AM Range
1 7 1.8 1.6 0.64–2.9 0.63 0.92 0.16–3.2
2 2 9.4 9.4 8.8–10 2.0 3.7 0.41–6.8
3 2a 17 17 15–19 19 19
4 5 14 19 1.7–28 2.7 3.6 1.0–7.4
5 6 5.0 5.2 2.6–6.6 1.4 3.5 0.20–9.8
6 2 2.4 2.7 1.2–3.8 4.2 4.3 3.7–4.8
Total 24 5.0 8.2 0.64–28 1.7 3.5 0.16–19

AM, arithmetic mean; GM, geometric mean.


aOne wood dust measurement was excluded.
Production of wood pellets 223

Table 3. Levels of monoterpenes (sum of α- and β-pinene and ∆3-carene) and of wood dust at static measurements at the nine test
sites
Test site Measurements Company no. Total monoterpenes (mg/m3) Wood dust (mg/m3)
(n)
GM AM Range GM AM Range
Control room 12 1–6 1.5 2.1 <0.85–4.4 a a <0.10–0.18
Shaving/sawdust storage 8 4–6 12 15 4.9–36 7.3 13 1.6–34
Dryer 2 5 5.9 5.9 5.1–6.7 1.2 1.4 0.72–2.1
Grinder 2 6 1.2 1.5 0.60–2.4 0.71 0.72 0.64–0.78
Press 12 1–6 7.5 21 <0.84–74 0.32 0.65 <0.10–2.2
Wood pellets storage 10b 2c, 3, 5, 6 2.5 7.6 <0.84–34 1.4 2.2 0.46–9.9
Briquette machine 1 3 42 42 0.49 0.49
Bagging 2 1 1.9 1.9 1.4–3.9 a a <0.12–0.64
Total 49 4.0 11 <0.84–74 0.56 2.9 <0.10–34

AM, arithmetic mean; GM, geometric mean.


aHalfor more of the measurements were under the detection limit.
bNine monoterpene measurements were done.
cMeasurements were done at two static sites.

Fig. 2. Air levels of wood dust at the six companies for the morning, afternoon and day shifts (n = 23).

estimated since the filter was overloaded and there- sampling of wood dust the air levels ranged from
fore it was excluded from all statistical analyses, <0.10 to 34 mg/m3 (Table 3). Low levels were meas-
tables and figures. For 10 of the participants the wood ured in the control room (<0.10–0.18 mg/m3) and
dust exposure exceeded the present Swedish OEL of high levels in the sawdust and shaving store (GM
2 mg/m3 (Fig. 2). No statistically significant (P = 7.3 mg/m3).
0.11) difference in levels of wood dust was seen
between the different companies. However, higher Measurements with the DataRAM
levels were often measured during the morning shift Measurements of dust with a DataRAM showed
compared with the afternoon shift and this difference large variations in peak exposures between workers,
was statistically significant (P = 0.04). The correl- within as well as between companies. The number of
ation between exposure to wood dust and mono- peaks (>0.4 mg/m3) recorded for each worker varied
terpenes was moderate (r = 0.44). For static between 4 and 49 over an 8 h working day. Peak
224 K. Edman et al.

Fig. 3. Measurements with the DataRAM. The line marks the OEL of 2 mg/m3.

values were observed at several working operations,


for example management of machines, bagging of
product, loading of raw material, sweeping and
cleaning of truck engines with compressed air (Fig.
3).
A high correlation (r = 0.89) was seen between the
parallel measurements of wood dust on a filter and
dust with a DataRAM. Levels measured with the
DataRAM were systematically lower than the levels
measured on the filter. At high levels of wood dust on
the filter (> 2 mg/m3) the DataRAM measurements
were in general 1/10 of the measurements of wood
dust on the filter (Fig. 4). However, at lower levels of
wood dust (<2 mg/m3) the levels measured with the
DataRAM varied from 0.008 to 0.8 of the levels of
wood dust on the filter.
Fig. 4. The ratio of the levels of wood dust measured with the
DataRAM and on a filter versus levels of wood dust measured
DISCUSSION on a filter (n = 17).

The personal exposures to monoterpenes were rela-


tively low while the levels of wood dust were high less. Compared with the other test sites, high levels
compared with the present OEL and previous studies
of monoterpenes were measured at the briquette
in the Swedish woodwork industry (Hedenstierna et
machine (42 mg/m3) in one of the companies. The
al., 1983; Eriksson and Levin, 1990; Eriksson et al.,
1996, 1997; Eriksson and Liljelind, 2000). The production of wood briquettes is a more mechanical
differences in levels of monoterpenes compared with process and the impact on the wood is higher. The
sawmills and joinery shops can probably be higher levels of wood dust might be due to the
explained by differences in the production processes. handling of shavings and sawdust in the production
In sawmills and joinery shops wood is split, which of wood pellets, which does not occur to the same
leads to breaking of cells and a higher release of extent in other woodwork industries.
monoterpenes. No splitting is done in the production Since the upper respiratory system is the target site
of pellets and the mechanical impact on the wood is for wood dust, measurements of inhalable dust would
Production of wood pellets 225

have been preferable. This was not done since the levels than wood dust on a filter, the DataRAM
OEL for wood dust in Sweden refers to total dust. In values cannot be compared with the OEL.
comparisons between total dust and inhalable dust it However, the DataRAM is a good instrument for
has been shown that the level of inhalable dust is on showing changes in exposure over time and can
average 2–4 times higher than total dust (Davies et therefore identify critical working tasks. Cleaning of
al., 1999; Lidén et al., 2000). truck engines with compressed air is a very critical
The difference in monoterpene concentrations working task with respect to wood dust exposure.
between the companies could be due to the fact that Since high exposure to wood dust can be linked to
the release of monoterpenes depends on how dry and specific working operations, a reduction in exposure
new the raw material is (Eriksson et al., 1997). This levels could probably be achieved by changes in
can probably explain the higher exposure to mono- working routines and by use of protective equipment.
terpenes that was noticed at company number 4. In Other exposures in the production of wood pellets
this company the raw material was transported via could be oxidized monoterpenes. Monoterpenes are
on-line pipes from a nearby planing mill into the easily oxidized by oxygen and ozone (Calogirou et
production area. This difference could also depend on al., 1999; Falk-Filipsson, 1995), with the main oxida-
differences in proportions of pine and spruce in the tion products being pinon aldehyde, pina ketone and
raw material, since pine releases more monoterpenes 3-caron aldehyde, from α-pinene, β-pinene and ∆3-
than spruce, which, for example, has been seen during carene, respectively. Pinon aldehyde and 3-caron
sawing of pine and spruce (Welling et al., 2001; aldehyde are both more reactive than the original
Rosenberg et al., 2002). However, the companies did monoterpene, while pina ketone is more stable
not report any differences in the proportions of pine (Calogirou et al., 1999). The health effects are
and spruce in their production and the supplier did supposed to be similar to the effects of the original
not have exact information of the composition. monoterpenes.
Different species of pine and spruce in the raw A known problem in storage of sawdust is allergic
material could probably also explain the low levels alveolitis, which is due to mould in damp sawdust.
that were measured in a Canadian study compared No problems with mould have been reported by the
with Swedish studies (Demers et al., 2000). companies. Since the turnover is very rapid and the
sawdust used is quite dry, mould should not be a
The personal wood dust measurements showed
problem. Another potential exposure is engine exhaust
considerable variation within and between workers.
from the trucks used. In studies on storage of wood
This could probably be explained by differences in
pellets, hexanal, acetone, carbon monoxide and meth-
working tasks and/or differences in working routines, anol have been identified. The level of hexanal was
which might also explain the higher level of wood 28 mg/m3 and measurements of hexanal are of
dust that was noticed during the morning shift interest since aldehydes in general are irritating to the
compared with the afternoon shift. From the working eyes, skin and airways (Svedberg and Galle, 2001).
diary no systematic differences were seen between Hydroperoxides of monoterpenes, especially ∆3-
working tasks for the morning and afternoon shifts. carene, are known to be allergic agents. However, no
No association between working tasks and levels of report on the occurrence of monoterpene hydro-
wood dust exposure could be noticed either. Differ- peroxides in the air in wood industries has, to our
ences in working tasks for morning and afternoon knowledge, been published. The resin in pine and
workers in different companies were noticed: at spruce contains other derivatives of terpenes, namely
companies numbers 1 and 4 monitoring from the resin acids. The major components are abietic and
control room dominated (34–71% of the working dehydroabietic acid, which are readily oxidized by
day), while in the other companies maintenance work air. The acids and especially the oxidized forms are
and repairs (39–60%) and driving of trucks (44–76%) suspected to cause contact allergy, occupational
were the most common working tasks. asthma and urticaria (Färm, 1997). Abietic acid, at a
A high correlation between the levels of dust meas- level between <0.05 and 370 µg/m3, has been identi-
ured with a DataRAM and wood dust measured on a fied in sawmills and lumber mills handling pine and
filter was seen, but the levels measured with the spruce (Demers et al., 2000; Teschke et al., 1999).
DataRAM were systematically lower than measure- Future studies within this kind of industry should
ments on the filter. Wood dust on the filter is a total focus on these substances.
dust measurement while the DataRAM mainly meas-
ures the respirable fraction, which could explain the CONCLUSION
difference between the two methods. In a study
comparing levels measured with a DataRAM with For 24 workers involved in the industrial production
respirable dust on the filter, a regression slope of ~1 of wood pellets, personal exposure to monoterpenes
was seen (Thorpe and Walsh, 2002). Since measure- was low and to wood dust high, compared with the
ments of dust with the DataRAM showed lower present Swedish OEL and previous studies in
226 K. Edman et al.

Swedish woodwork industries. Since the DataRAM Eriksson KA, Levin JO, Sandström T, Lindström-Espeling K,
can identify critical working tasks with high wood Lindén G, Stjernberg NL. (1997) Terpene exposure and
respiratory effects among workers in Swedish joinery shops.
dust exposures, a reduction in exposure levels could Scand J Work Environ Health; 23: 114–20.
probably be achieved by changes in working routines Falk-Filipsson A. (1995) Toxicokinetics and acute effects of
and by the use of protective equipment. inhalation exposure to monoterpenes in man. Arbete och
Hälsa 1995: 3. Solna: National Institute of Working Life.
Färm G. (1997) Contact allergy to colophony: clinical and
Acknowledgments—Financial grants from the Department of
experimental studies with emphasis on clinical relevance,
Occupational and Environmental Medicine, Örebro University
PhD thesis, Karolinska Institut, Stockholm.
Hospital, Örebro, Sweden, and the Swedish Energy Agency,
Eskilstuna, Sweden, are gratefully acknowledged. We would Fengel D, Wegener G. (1983) Wood: chemistry, ultrastructure,
also like to thank Lennart Andersson, Krister Berg, Britt-Marie reactions. Berlin: Walter de Gruyter & Co. p. 186.
Isaksson, Birgitta Linder and Håkan Westberg for their help in Hedenstierna G, Alexandersson R, Wimander K, Rosén G.
the field, laboratory and with the article. Last, but not least, we (1983) Exposure to terpenes: effects on pulmonary function.
would like to thank all the companies for participating in the Int Arch Occup Environ Health; 51: 191–8.
study. Hornung RW, Reed LD. (1990) Estimation of average concen-
tration in the presence of nondetectable values. Appl Occup
Environ Hyg; 5: 46–51.
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