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Know How much

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caffeine you are taking in with each cup of tea!

Project Prepared By:

Ankit Bahuguna XII-A o!! "um#er: $o%e!y Pu#!ic &r' &ec' &choo! (P')' *ihar+
)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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0ir.t of a!! I wou!d !ike to thank my cou.in #rother Mr. Neeraj Naithani e.earch &cho!ar )epartment of ,hemi.try1 II- oorkee for hi. contri#ution in thi. .tudy' I wou!d a!.o !ike to thank my ,hemi.try -eacher Ms. Rampal in pro%iding a he!ping hand in making my project tit!ed 2Determination Of Caffeine In Tea Samples3' )i.c!aimer.: -he error. idiocie. and incon.i.tencie. remain my own'

Ankit Bahuguna
(Name an signature of the stu ent!

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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It i. here#y certify that1 the origina! and genuine re.earch work i. carried out to in%e.tigate a#out the .u#ject matter and the re!ated data co!!ection and ha. #een comp!eted .o!e!y and .ati.factori!y #y thi. .tudent regarding the Project tit!ed 2Determination Of Caffeine In Tea Samples3'

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&ignature
(&u#ject -eacher+
)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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,6"-7"-&
1' /' 4' 5' 9' :' ;' Introduction -heory 8.e. of ,affeine 7ffect. of ,affeine Procedure 6#.er%ation. e.u!t

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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I" - 6 ) 8 , - I6 " -ea i. the mo.t common!y and wide!y u.ed .oft #e%erage in the hou.eho!d' It act. a. a .timu!ant for centra! ner%ou. .y.tem and .ke!eta! mu.c!e.' -hat i. why tea remo%e. fatigue1 tiredne.. and headache' It a!.o increa.e. the capacity of thinking' It i. a!.o u.ed for !owering #ody temperature' -he principa! con.tituent of tea1 which i. re.pon.i#!e for a!! the.e propertie.1 i. the a!ka!oid-caffeine' -he amount of caffeine in tea !ea%er. %arie. from .amp!e to .amp!e' 6rigina!!y it wa. thought that caffeine i. re.pon.i#!e for the ta.te and f!a%our of tea' But pure caffeine ha. #een found to #e a ta.te!e.. whi!e .u#.tance' -herefore1 the ta.te and f!a%our of tea i. due to .ome other .u#.tance pre.ent in it' -here i. a !itt!e dou#t that the popu!arity of the <anthene. #e%erage. depend. on their .timu!ant action1 a!though mo.t peop!e are unaware
)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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of any .timu!ation' -he degree to which an indi%idua! i. .timu!ated #y gi%en amount of caffeine %arie. from indi%idua! to indi%idua!' 0or e<amp!e1 .ome peop!e #oa.t their a#i!ity to drink .e%era! cup. of coffee in e%ening and yet .!eep !ike a !ong1 on the other hand there are peop!e who are .o .en.iti%e to caffeine that e%en a .ing!e cup of coffee wi!! cau.e a re.pon.e #oarding on the to<ic' -he <anthene #e%erage. a!.o create a medica! pro#!em' -hey are dietary of a .timu!ant of the ,"&' 6ften the phy.ician. face the =ue.tion whether to deny caffeinecontaining #e%erage. to patient. or not' In fact chi!dren are more .u.cepti#!e than adu!t. to e<citation #y <anthene.' 0or thi. rea.on1 tea and coffee .hou!d #e e<c!uded from their diet' 7%en cocoa i. of
)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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dou#tfu! %a!ue' It ha. a high tannin content may #e a. high a. 9> mg per cup' After a!! our main .tre.. i. on the pre.ence of caffeine in <anthene #e%erage. and .o in thi. project we wi!! .tudy and o#.er%e the =uantit of %aryin i differen y caffeine g n t .amp!e of tea . !ea%e.'

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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-h e o r y

-he mo.t important methy!ated a!ka!oid that occur. natura!!y i. caffeine' It. mo!ecu!ar formu!a i. ,?H1>"56/' It. I8PA, name i. 11 41 ;-trimethy!<anthene and common name i. 1-methy!ated thio#romine'

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)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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Pure!y it i. white1 cry.ta!!ine .o!id in the form of need!e.' It. me!ting point i. 1/4>c' It i. the main acti%e princip!e component of tea !ea%e.' It i. pre.ent in tea !ea%e. up to 4A and can #e e<tracted #y fir.t #oi!ing the tea !ea%e. with water which di..o!%e. many g!yco.ide compound. in addition to caffeine' -he c!ear .o!ution i. then treated with !ead acetate to precipitate the g!yco.ide compound. in the form of !ead comp!e<' -he c!ear fi!trate i. then e<tracted with e<tract. caffeine #ecau.e it i. more .o!u#!e in it then water'

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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8.e. of ,affeine

1' In medicine1 it i. u.ed to .timu!ate1 centra! ner%ou. .y.tem and to increa.e f!ow of urine' /' Becau.e of it. .timu!ating effect.1 caffeine ha. #een u.ed to re!ie%e fatigue' But it i. dangerou. and one may co!!ap.e if not con.ume. it under certain !imit' 4' ,affeine i. a!.o u.ed in ana!ge.ic ta#!et.1 a. it i. #e!ie%ed to #e a pain re!ie%er' It i. a!.o #eneficia! in migraine.'

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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7ffect. of ,affeine
1' It i. p.ycho - .timu!ant' /' It impro%e. phy.ica! and menta! a#i!ity' 4' It. effect in !earning i. dou#tfu! #ut inte!!ectua! performance may impro%e where it ha. #een u.ed to reduce fatigue or #oredom' 5' Bhen admini.tered interna!!y1 it .timu!ate. heart and ner%ou. .y.tem and a!.o act. a. diuretic' 6n the contrary their e<ce..i%e u.e i. harmfu! to dige.tion and their !ong u.e !ead. to menta! retardation'

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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Procedure
0ir.t of a!!1 9> gram. of tea !ea%e. were taken a. .amp!e and 19> m! of water wa. added to it in a #eaker' -hen the #eaker wa. heated up to e<treme #oi!ing' -he .o!ution wa. fi!tered and !ead acetate wa. added to the fi!trater1 !eading to the formation of a curdy #rown co!oured precipitate' Be kept on adding !ead acetate ti!! no more precipitate ha. #een formed' Again .o!ution wa. fi!tered' "ow the fi!trate .o o#tained wa. heated unti! it had #ecome 9> m!' -hen the .o!ution !eft wa. a!!owed to coo!' After that1 /> m!' of ch!oroform wa. added to it' &oon after1 two !ayer. appeared in the .eparating funne!'
)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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Be .eparated the !ower !ayer' -he .o!ution then e<po.ed to atmo.phere in order to a!!ow ch!oroform to get e%aporated'

-he re.idue !eft #ehind wa. caffeine' -hen we weighed it and recorded the o#.er%ation.' &imi!ar procedure wa. performed with different .amp!e. of tea!ea%e. and =uantity of caffeine wa. o#.er%ed in them

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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1.Red Label Tea (Brooke Bond) Beight of china di.h 5:':>gm. Beight of china di.h 5;'/>gm.' with precipitate Amount of caffeine >':>gm. 2.Yellow Label Tea (Lipton) Beight of china di.h 5:':>gm. Beight of china di.h 5;'19gm.' with precipitate Amount of caffeine >'99gm.

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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3.Green Label Tea (Lipton) Beight of china di.h Beight of china di.h with precipitate Amount of caffeine 5:':>gm.' 5;'>9gm.' >'59gm.'

1' Cuantity of caffeine in ed !a#e! tea i. :>mg' D.amp!e of 9> gm' /' Cuantity of caffeine in ye!!ow !a#e! tea i. 99mg'D.amp!e of 9> gm'
4'

Cuantity of caffeine in green !a#e! tea i. 59mg'D.amp!e of 9> gm'

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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Eraphica!!y p!otting %ariou. tea .amp!e. in accordance with the amount of caffeine pre.ent in them we pre.ent a .tunning find:

60 mg RED 0.6 0.5 0. 0.# 0.$ 0.% 0

55 mg YELLOW

5 mg !REE"

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+

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Order of quantities of

affeine in

different sa!ples of tealea"es

)etermination of ,affeine in -ea &amp!e. Ankit Bahuguna (XII-A+