ARTIGOS Impact of chemical changes on the sensory characteristics of coffee beans during storage

Mery Yovana Rendn a, Terezinha de Jesus Garcia Salva b, Neura Bragagnolo a, a Faculty o Food !ngineering, "niversity o #a$%inas &"N'#(M)*, +,-.,/.01 #a$%inas, S2o )aulo, Brazil b (grono$ic 'nstitute o #a$%inas &'(#*, #o ee #enter, )3 Bo4, 1., +,-+1/56- #a$%inas, S2o )aulo, Brazil

Abstract: Sensory changes during the storage o co ee beans occur $ainly due to li%id o4idation and are res%onsible or the loss o co$$ercial value7 This 8or9 ai$ed to veri y ho8 sensory changes o natural co ee and %ul%ed natural co ee are related to the o4idative %rocesses during +: $onths o storage7 ;uring this %eriod, changes in the content o ree atty acids &+7<=,7. $g>g oil*, TB(RS values &.7.=+-71 n$ol M;(>g*, and carbonyl grou%s &170=,7: n$ol>$g o %rotein* occurred7 The intensity o ??rested co ee lavour@@ in the co ee bre8 increased &17+=076* and :/ca eoylAuinic acid concentration decreased &:71=<70 g>+-- g*7 Bosses 8ere also observed in seed viability, colour o the beans and cellular structure7 (ll the results o the che$ical analyses are coherent 8ith the o4idative %rocess that occurred in the grains during storage7 There ore, o4idation 8ould be also res%onsible or the loss o cellular structure, seed viability and sensory changes7 Keywords: Coffea Arabica, TBARS, Lipid oxidation, Carbonyl groups, Chlorogenic, acid, Rested coffee, Coffee quality. Food Che istry !"# $%&!"' %#()%*+ Divergncia gen tica entre cafeeiros do germoplasma !aragogipe
Janine Magalh2es Guedes &+C*D ;iego JEnior Martins Filela &+*D Juliana #osta Rezende &1*D Feli%e Bo%es Silva &,*D #Gsar !lias Botelho &1*D Sa$uel )ereira #arvalho &+* &+* "niversidade Federal de Bavras &"FB(*, ;e%arta$ento de (gricultura, #ai4a )ostal, ,-,6, ,61--/ --- Bavras &MG*, Brasil7 &1* !$%resa de )esAuisa (gro%ecuHria de Minas Gerais, "nidade Regional do Sul de Minas, #ai4a )ostal, +60, ,61--/--- Bavras &MG*, Brasil7 &,* !$%resa de )esAuisa (gro%ecuHria de Minas Gerais, "nidade Regional da Iona da Mata, #a$%us "FF, ,0:6+/--- FiJosa &MG*, Brasil7 &C* (utor corres%ondenteK LanineMguedesNyahoo7co$7br RecebidoK +0>out7>1-+1D (ceitoK +,>Lun7>1-+,

Resumo: !ste trabalho obLetivou identi icar a divergOncia genGtica entre acessos de ca eeiro do gru%o Maragogi%e, situados no Banco (tivo de Ger$o%las$a de #a G do !stado de Minas Gerais, no $unicP%io de )atrocPnio &MG*, %or $eio de anHlises $ultivariadas7 Fora$ avaliados 16 descritores $or o/agronQ$icos, e a distRncia generalizada de Mahalanobis oi usada %ara Auanti icar a divergOncia genGtica entre os acessos7 #o$o estratGgias de agru%a$ento, ora$ e$%regados o agru%a$ento de Tocher, o $Gtodo hierHrAuico "n8eighted )air/Grou% Method "sing (rith$etic (verages &")GM(* e a anHlise de variHveis canQnicas7 ( anHlise de agru%a$ento %elos $Gtodos de Tocher e ")GM( se%arara$ os acessos e$ trOs e seis gru%os, res%ectiva$ente7 ( anHlise da contribuiJ2o relativa de cada caracterPstica %ara a dissi$ilaridade genGtica destacou as caracterPsticas intensidade da ondulaJ2o da olha, Auantidade de in lorescOncia %or a4ila oliar e %rodutividade $Gdia co$o as Aue $ais contribuPra$ %ara a obtenJ2o da divergOncia genGtica7 3bservou/se Aue as trOs %ri$eiras variHveis canQnicas e4%licara$ $ais de 5-S da variRncia total contida no conLunto das caracterPsticas analisadas7 ( co$binaJ2o entre os acessos MG-+06 e MG-+6- G a $ais

%ro$issora e$ u$ %ossPvel cruza$ento %ara uso i$ediato e$ %rogra$as de $elhora$ento7 ,ala-ras.cha-e/ Coffea arabica, an0lise ulti-ariada, elhora ento gen1tico. Bragantia, Ca pinas, -. #%, n. %, p.!%#.!2%, %&!2 "ffect on the physico#chemical properties of liberica green coffee beans under ambient storage
's$ail, '7, (nuar, M7 S7 and Sha$sudin, R7 3epart ent of ,rocess and Food 4ngineering, Faculty of 4ngineering, 5ni-ersiti ,utra 6alaysia, "2"&& Serdang, Selangor, 6alaysia
C

AbstractK Green co ee beans are stored or a certain %eriod and under certain conditions until they are inally utilized7 The storage %eriod $ay de%end on custo$er de$and 8hile the storage conditions de%end on 8here the co ee beans are stored7 Thus, this research e$%hasizes the %hysico/che$ical changes that occur in Biberica co ee beans during storage under the Malaysian cli$ate &average te$%erature and relative hu$idity o 157,,T# and 6+76:S res%ectively*7 The changes in the %hysico/che$ical &co ee size, $ass, densities, colour, %ro4i$ate analysis, sucrose, chlorogenic acid content* and $icrobiological &yeast and $ould count* %ro%erties 8ere evaluated during eight $onths o storage7 ( ter the storage, the %hysical %ro%erties o the co ee changed as the co ee beans e4%anded in size, reduced in $ass and density and beca$e brighter in colour7 #hanges in the che$ical %ro%erties 8ere also detected 8here the $oisture decreased and the ash content increased7 'n addition, the sucrose level 8as ound to decrease 8ith a corres%onding increase in chlorogenic acid7 ;uring storage, the counts o yeast and $ould 8ere reduced7 Model eAuations describing the changes in the %ro%erties 8ere develo%ed7 The overall conclusion 8as that the co ee beans reduced in Auality during storage7 Keywords: Coffee beans, liberica, physical properties, che ical properties, storage. 7nternational Food Research 8ournal %&$!'/ %99.%+" $%&!2' $haracteri%ation and !odeling of the Interactions bet&een $offee Storage 'roteins and 'henolic $ompounds
Mosta a (li,U Tho$as Vo$ann,U Jan9a Wreisel,X Mah$oud Whalil,U Ral )uhl$ann,X Vans/)eter Wruse,U and Varshadrai Ra8elC,U U'nstitute o Nutritional Science, "niversity o )otsda$, (rthur/Scheunert/(llee ++</++0, +<::. Nuthetal, )otsda$, Ger$any X;eutsche !4tra9t9a ee G$bV, #a eastraYe +, +1,<6 Berlin, Ger$any CS Su%%orting 'n or$ation

A(STRA$T: This study addresses the interactions o co ee storage %roteins 8ith co ee/s%eci ic %henolic co$%ounds7 )rotein %ro iles o #o ea arabica and #o ea cane%hora &var7 robusta* 8ere co$%ared7 MaLor %henolic co$%ounds 8ere e4tracted and analyzed 8ith a%%ro%riate $ethods7 The %oly%henolZ%rotein interactions during %rotein e4traction have been addressed by di erent analytical setu%s [reversed/%hase high/ %er or$ance liAuid chro$atogra%hy &R)/V)B#*, sodiu$ dodecyl sul ateZ %olyacryla$ide gel electro%horesis &S;SZ)(G!*, $atri4/assisted laser desor%tion ionizationZti$e o lightZ$ass s%ectro$etry &M(B;'ZT3FZMS*, and Trolo4 eAuivalent antio4idant ca%acity &T!(#* assays\, 8ith ocus directed to8ard identi ication o covalent adduct or$ation7 The results indicate that #7 arabica %roteins are $ore susce%tible to these interactions and the %oly%henol o4idase activity see$s to be a crucial actor or the or$ation o these addition %roducts7 ( tentative allocation o the $odi ication ty%e and site in the %rotein has been atte$%ted7 Thus, the irst available in silico $odeling o $odi ied co ee %roteins is re%orted7 The e4tent o these

$odi ications $ay contribute to the structure and unction o ]co ee $elanoidins^ and are discussed in the conte4t o co ee lavor or$ation7 KEYWORDS: Coffee beans, storage proteins, phenolic co pounds, antioxidants, protein:phenol interactions, odeling 8. Agric. Food Che . %&!%, +&, !!+&!:!!+&* Test of e)udates color hues for evaluating the physiological potential of coffee *Coffea arabica +,- seeds_
)aulo #esar Vilst`C, ;enise #unha Fernandes dos Santos ;ias`, !veline Mantovani (lvarenga`, Bruna Buiza de Souza`

A(STRA$T # #o ee seeds have slo8 and irregular ger$ination, losing ast their viability during storage, and the standard ger$ination test o these seeds reAuires at least ,- days7 Besides, the results $ay not re lect the actual %hysiological Auality o these seeds7 The obLective o this 8or9 8as to develo% a ast and %ractical test or evaluating the viability o co ee seeds, 8hich is based on the inter%retation o di erent color hues o e4udates ro$ seeds7 #o ee seeds o the cultivar #atuai << ro$ si4 lots 8ere sub$itted to ger$ination, accelerated aging, and electrical conductivity tests7 'n the e4udates color hue test, co ee seeds 8ithout the %arch$ent and the silvery %ellicle & our re%lications o +- seeds each* 8ere distributed on to% o %a%er to8els $oistened and then $aintained into a ger$inator, at 1: T# or 1<, <., 61, 50, and +1- h7 Three classes o color hues 8ere establishedK colorless, light color hue, and dar9 color hue, assigning the values o -, +, and ,, or each class, res%ectively7 The %ro%osed e4udates color hue test can be reco$$ended or the ast assess$ent o viability or co ee seeds7 The $ost %ro$ising results 8ere obtained or seeds 8ith +1S $oisture content, a ter i$bibition %eriods o 61, 50, and +1- hD and 8ith ,-S $oisture content, a ter i$bibition %eriods o 61 and +1- h7 Index terms: leaching, seed quality, seed analysis, ger ination, -igor. Revista Brasi eira de Sementes, -ol. 2", n; % p. %!% . %!#, %&!% ./A+IDAD" S"0SORIA+ " $O+ORA12O DO $A34 ("0"3I$IADO AR!A5"0ADO "! "!(A+AG"0S 64R!"TI$AS
Fabiana #ar$anini Ribeiro_D Buisa )ereira Figueiredo`D FlHvio Meira BorG$aD Gerson Silva Gio$o<D Sa$uel Ra$alio #oste e #ol%a: + !ngb (grQno$a, ;outoranda e$ !ngenharia (grPcola, ;!G = "FB(, abianacar$aniniNyahoo7co$7br 1 !ngb de (li$entos, Mestranda e$ #iOncia dos (li$entos, ;#( = "FB(, lu%e iNg$ail7co$ , )ro essor )s/;outor do ;e%arta$ento de !ngenharia = "FB(, laviobore$Nu la7br < )esAuisador do 'nstituto (gronQ$ico de #a$%inas, #a$%inas/S), gsgio$oNyahoo7co$7br : !studante de GraduaJ2o e$ !ngenharia (grPcola / "FB(, sa$uelcosteNyahoo7co$7br

R"S/!OK ( busca de novas or$as de acondiciona$ento %ara au$entar o te$%o de ar$azena$ento dos gr2os de ca G $antendo sua Aualidade G de e4tre$a i$%ortRncia7 Neste trabalho oi estudado o e eito do $Gtodo de acondiciona$ento de ca G bene iciado a%s +1 $eses de ar$azena$ento7 3s trata$entos constituPra$/se de u$ esAue$a atorial 1 4 1 big/bags her$Gticos se$ e co$ inLeJ2o de #31, at$os era $odi icada e at$os era controlada res%ectiva$ente e duas %osiJces de a$ostrage$K su%erior e in erior, e $ais dois trata$entos adicionaisK saco de Luta e Grain)ro7 ( Aualidade do ca G oi avaliada %or $eio de u$a avaliaJ2o Aualitativa da cor e anHlise sensorial7 ( avaliaJ2o Aualitativa da coloraJ2o dos gr2os oi realizada %or classi icadores treinados Aue atribuPra$ as deno$inaJces verde/azulada, verde, a$arelada, $anchada e branAueada ds a$ostras codi icadas7 Na anHlise sensorial o %ainel sensorial oi co$%osto %or degustadores treinados, LuPzes da S#((, classi icara$ as a$ostras co$o de sa ra atual ou sa ra re$anescente7 (%s +1 $eses de

ar$azena$ento os gr2os acondicionados e$ big/bags her$Gticos a%resentara$ altas reAeOncias %ara a classi icaJ2o Auanto d coloraJ2o verde e sa ra atual7 !a avras c"ave: <ualidade, Coffea ar0bica L., at osfera controlada. =77 Si p>sio de ,esquisa dos Caf1s do Brasil %% a %9 de Agosto de %&!!, Arax0 . 6? $ondicionamento fisiol7gico em matri% s7lida de sementes de caf *Coffea arabica +,- com e sem pergaminho8
#arlos (lberto Machado #arvalho1, Renato Mendes Gui$ar2es,C, Silva, Tanis$are Tatiana de (l$eida

R"S/!O # 3bLetivou/se nessa %esAuisa avaliar a ger$inaJ2o e o vigor das se$entes de Coffea arabica B7 cv7 #atuaP Fer$elho '(# 55, sub$etidas ao condiciona$ento isiolgico e$ $atriz slida co$ e se$ %erga$inho %or di erentes %erPodos de te$%o7 3 delinea$ento e4%eri$ental oi e$ esAue$a atorial 1 4 <, considerando, na realizaJ2o do condiciona$ento isiolgico, se$entes co$ e se$ %erga$inho e o te$%o de condiciona$ento das se$entes &-, <, . e +1 dias*7 (s se$entes ora$ condicionadas a ,- o#, e$ $atriz slida, co$ aLuste de +--S da ca%acidade de retenJ2o de Hgua7 (%s os %erPodos de condiciona$ento, retirou/se $anual$ente o %erga$inho %ara a realizaJ2o dos testes e, e$ seguida, avaliou/se a u$idade, a ger$inaJ2o e o vigor7 Feri icou/se Aue a %resenJa do %erga$inho n2o in luencia os e eitos do condiciona$ento isiolgico na Aualidade das se$entes7 3 condiciona$ento isiolgico durante Auatro e oito dias, n2o $elhora a Aualidade das se$entes de ca G, no entanto, durante +1 dias, hH %reLuPzo na ger$inaJ2o e no vigor7 Ter os para indexa@Ao/ caf1, ger ina@Ao, -igor. Revista Brasi eira de Sementes, -ol. 2", n; ! p. &(" . &(*, %&!% 'RI!I0G "! !ATRI5 S9+IDA D" S"!"0T"S D" $A34 AR!A5"0ADAS $O! A+TA /!IDAD"
#arlos (lberto Machado #arvalho+, Renato Mendes Gui$ar2es1, Tanis$are Tatiana de (l$eida Silva, &RecebidoK 0 de Lulho de 1-+-D aceito 1, de $aio de 1-++*

R"S/!O: 3 conteEdo de Hgua das se$entes e a te$%eratura a eta$ os %rocessos biolgicos, deter$inando variados resultados Auando as se$entes s2o sub$etidas a di erentes nPveis desses atores7 fuando as se$entes s2o sub$etidas ao %ri$ing, es%era$/se res%ostas e$ raz2o da $elhoria na Aualidade isiolgica dessas se$entes, $as, e$bora %ro$issor, esse %rocedi$ento ainda se encontra e$ ase e4%eri$ental e$ se$entes de ca G7 #o$ obLetivo de avaliar os e eitos do %ri$ing e$ $atriz slida e$ trOs di erentes te$%eraturas sobre o dese$%enho de se$entes de ca G &Coffea arabica B7* ar$azenadas %or atG doze $eses, realizou/se a %resente %esAuisa no Baboratrio #entral de (nHlise de Se$entes do ;e%arta$ento de (gricultura da "niversidade Federal de Bavras, Bavras = MG7 Se$entes co$ ,0,-S de u$idade ora$ classi icadas, tratadas, acondicionadas e$ e$balagens de %olietileno e ar$azenadas e$ cR$ara ria %or atG +1 $eses7 !$ intervalos tri$estrais, as a$ostras de se$entes ora$ sub$etidas ao %ri$ing e$ $atriz slida, e$ te$%eraturas de 1- o#, ,- o# e <- o#, %or 0 dias7 "$a teste$unha, se$ %ri$ing, oi ta$bG$ avaliada7 3 condiciona$ento isiolgico e$ $atriz slida de se$entes de C. arabica7 realizado %or 0 dias nas te$%eraturas de 1- g# e ,- g# avorece a e$ergOncia de %lRntulas e$ se$entes ar$azenadas %or 5 $eses e, sob te$%eratura de <- g#, G %reLudicial ds se$entes de C. arabica7 ,ala-ras.cha-e/ Coffea arabica L., ger ina@Ao, -igor. Coffee Science, La-ras, -. +, n. %, p. (*.!&+, aioBago. %&!!

The effect of storage conditions on coffee seed and seedling :uality

S7;7F7F7 ;( R3S(+, (7M7 #(RF(BV31, M7B7 Mc;3N(B;,, !7R7F7 F3N )'NV31, (7)7 S'BF(1 (N; (7;7 F!'G(1 + !$bra%a, )A!B, (v7 h, Norte & inal*, !d7 Sede, #!) 6-66-/5-+, BrasPlia, ;F, Brazil &!/$ailK sttelaveigaNdag7u la7br* % "niversidade Federal de Bavras, #!) ,61--/---, Bavras, MG, Brazil , Seed Biology )rogra$, ;e%art$ent o Vorticulture and #ro% Science, 3hio State "niversity, 1-1+ #o ey Rd, #olu$bus, 3V <,1+-, "S( $Accepted Cctober %&!&'

Summary: 3btaining co$$ercially use ul co ee seedlings is hindered by slo8, uneven ger$ination and lo8 tolerance to desiccation as 8ell as reduced co ee seed longevity7 #o ee seeds have been considered recalcitrant, orthodo4 and even inter$ediate 8ith varying results7 #urrent reco$$endations suggest that co ee seeds can be sa ely stored bet8een +-/++S 787b7 at +:g#7 Vo8ever, a ter drying and storage, co ee seeds lose vigour, and seeds stored a ter drying cannot be used or %roducing seedlings7 #o ee seedling %roducers usually so8 seeds i$$ediately or lightly dry the$ a ter harvest or a short storage %eriod, i necessary7 't is highly desirable that seeds are stored sa ely to o%ti$ize co ee seedling %roduction at the a%%ro%riate ti$e and season 8ith ideal cli$atic conditions or %lanting in the ield7 The obLective o this study 8as to deter$ine the Auality o co ee seedlings %roduced ro$ seeds stored 8ith high, $ediu$ and lo8 $oisture levels under her$etic conditions at +- and 1-g#7 Seed and seedling Auality 8ere assessed be ore and a ter nine $onths storage7 3nly the ger$ination o seeds harvested at the cherry stage, evaluated be ore and a ter the storage at +-g#, 8as not a ected by $oisture content, but these seeds lost vigour and did not %roduce suitable seedlings or %lanting 8hen stored or nine $onths7 Seedlings %roduced in the nursery ro$ seeds 8ith <6 and +1S $oisture content %er or$ed the sa$e as those ro$ greenish/yello8 seeds, but they %roduced a lea area that 8as ive ti$es s$aller, a ste$ height three ti$es shorter, and had +76 ti$es e8er %airs o true leaves than co ee seedlings %roduced ro$ resh seeds7 Storage at 1-g# 8as not suitable or co ee seeds, es%ecially those at +.S $oisture content 8hose Auality declined drastically7 These results suggest that co ee seeds do not tolerate desiccation and that their storage behaviour classi ication should be revie8ed7 Seed Science and Technology, =olu e 2(, Du ber !, April %&!! , pp. !9!.!+"$!"' $ARA$T"RI5A12O D" I0TROD/1;"S D" C# C$%E!&OR$ DO (A0$O D" G"R!O'+AS!A DO IA$ ./A0TO AO T"OR D" $A3"<0A 0OS GR2OS
M7 Bernadete Silvarolla`D Julieta (ndrGa Silva de (l$eida`D FalGria #ristina Barbosa #ar$azinia _Trabalho inanciado %elo #onsrcio Brasileiro de )esAuisa e ;esenvolvi$ento do #a G = #B)i;>#a G7 ` bernadetNiac7s%7gov7brD LulietasaNiac7s%7gov7br 1 )esAuisador do #entro de #a G ](lcides #arvalho^ = 'nstituto (gronQ$ico de #a$%inas, valeriaMcar$aziniNyahoo7co$7br , Bolsista do #onsrcio Brasileiro de )esAuisa e ;esenvolvi$ento do #a G = #B)i;>#a G

R"S/!O: ( ca ePna G u$ dos %rinci%ais alcalides de uso $edicinal7 3 estado de alerta %or ela %roduzido G o e eito $ais es%erado %elos consu$idores de bebidas co$o o ca G, guaranH, etc7 ( literatura $ostra Aue as es%Gcies C. arabica e C. canephora di ere$ Auanto aos teores $Gdios do alcalide nos gr2os7 !ntretanto, %ara u$a $aior e4%loraJ2o dos recursos genGticos dis%onPveis nos Bancos de Ger$o%las$a co$ vistas ao $elhora$ento genGtico hH necessidade de investigaJ2o no sentido de sua caracterizaJ2o Auanto a atributos $or olgicos, AuP$icos e agronQ$icos7 Neste sentido o %resente trabalho relata resultados de dosagens de ca ePna &S bs* e$ gr2os de cerca de ++: genti%os de C. canephora das cultivares Wouilou e Robusta do Banco de

Ger$o%las$a do '(#7 (s introduJces de C. canephora do ti%o Robusta a$ostradas a%resentara$ variaJ2o entre +,:: a ,,-:S e as do ti%o Wouilou de +,5. a ,,<+S7 !a avras'c"ave: Coffea canephora, cafeEna, caf1 robusta, recursos gen1ticos,
F' Si$%sio de )esAuisa dos #a Gs do Brasil

ARTIGOS "=TRAS Ade:ua>?o da metodologia do teste de tetra%7lio para sementes de caf rec m colhidas e arma%enadas
(line da #onsolaJ2o Sa$%aio #le$ente+, Maria Baene Moreira de #arvalho1, Renato Mendes Gui$ar2es1

R"S/!O: ( ger$inaJ2o de se$entes de ca G ocorre de or$a lenta e desuni or$e e o teste de tetrazlio &TI* %ode ser u$a alternativa rH%ida %ara a avaliaJ2o da viabilidade, desde Aue os %rocedi$entos de %re%araJ2o e e$bebiJ2o das se$entes esteLa$ estabelecidos7 3 trabalho oi realizado e$ duas eta%asK na %ri$eira, se$entes recG$/ colhidas de cinco cultivares de ca eeiro ora$ sub$etidas a Auatro $etodologias %ara re$oJ2o do %erga$inhoK e4traJ2o $anual ou utilizaJ2o de hi%oclorito de sdio &Na#l3 a :S* e dois %erPodos de e$bebiJ2o das se$entes e$ Hgua &,0 e <. horas* %ara a retirada dos e$brices7 Na segunda ase, oi co$%arada a re$oJ2o $anual e a utilizaJ2o de Na#l3 %ara a eli$inaJ2o do %erga$inho de se$entes ar$azenadas %ara realizaJ2o $ensal do teste de tetrazlio durante seis $eses7 3 hi%oclorito de sdio n2o G e iciente %ara a e4traJ2o total do %erga$inho e a eta negativa$ente os resultados do teste de TI e$ se$entes de ca G co$ bai4a u$idade7 3 te$%o de e$bebiJ2o de <. horas acilita a e4traJ2o dos e$brices e n2o a eta os resultados do teste7 3 teste de tetrazlio e$ se$entes de ca G, co$ di erentes nPveis de u$idade e deterioraJ2o, deve ser realizado co$ a re$oJ2o $anual dos %erga$inhos7 Ter os para indexa@Ao/ Coffea arabica, -iabilidade, perga inho. $Recei-ed in ay %, %&!% and appro-ed in Fuly 2, %&!%' CiGnc. agrotec., La-ras, -. 2+, n. ", p. "!9."%2, Ful.Bago., %&!% 'erfis proteicos e desempenho fisiol7gico de sementes de caf submetidas a diferentes m todos de processamento e secagem
JosG VenriAue da Silva Taveira&+*, Sttela ;ellyzete Feiga Franco da Rosa&1*, FlHvio Meira BorG$&+*, Gerson Silva Gio$o&,* e Reni Saath&,* &+*"niversidade Federal de Bavras, ;e%arta$ento de !ngenharia, #ai4a )ostal ,-,6, #!) ,61-- ---Bavras, MG7 !-$ailK henriAuetaveiraNyahoo7co$7br, laviobore$Ndeg7u la7br &1*!$bra%a #a G, (venida h, Norte &Final*, )arAue !staJ2o Biolgica, #!) 6-66--5-+ BrasPlia, ;F7 !-$ailK sttela7rosaNe$bra%a7br &,*'nstituto (gronQ$ico, (venida Bar2o de 'ta%ura, no +7<.+, #ai4a )ostal 1., #!) +,-1--56- #a$%inas, S)7 !-$ailK gsgio$oNyahoo7co$7br, reniagricolaNyahoo7co$7br

Resumo @ 3 obLetivo deste trabalho oi avaliar os %er is %roteicos e o dese$%enho isiolgico de se$entes de ca G sub$etidas a di erentes $Gtodos de %rocessa$ento e secage$7 Fora$ avaliados os %rocessa$entos %or via seca e E$ida, e as secagens natural, e$ terreiro, e arti icial a 0-T#, ou a 0-T# atG ,-S de u$idade e <-T# atG teor inal de ++S &base E$ida*7 (%s sere$ %rocessadas e secadas, as se$entes ora$ avaliadas Auanto ao dese$%enho isiolgico e sub$etidas a anHlises bioAuP$icas, %or $eio da eletro orese de %rotePnas resistentes ao calor B!( &]late e$bryogenesis abundant^* e das enzi$as su%er4ido dis$utase, catalase, %ero4idase, esterase, %oli enolo4idase, isocitrato desidrogenase, Hlcool desidrogenase e $alato desidrogenase7 3 %er il %roteico de se$entes de ca G G a etado %elo $Gtodo de %rocessa$ento e de secage$7 3s ca Gs %rocessados %or via E$ida a%resenta$ $aior

tolerRncia d secage$ = revelada %ela $aior atividade de enzi$as antio4idativas e %elo $elhor dese$%enho isiolgico = do Aue os %rocessados %or via seca7 ( atividade de %rotePnas resistentes ao calor e de enzi$as antio4idantes G variHvel %ro$issora %ara di erenciar a Aualidade dos ca Gs sub$etidos a di erentes $aneLos %s-colheita7 Ter os para indexa@Ao/ Coffea arabica, enHi as antioxidati-as, aneFo p>s - colheita, proteEnas L4A, proteEnas resistentes ao calor. ,esq. agropec. bras., BrasElia, -."#, n.!&, p.!9!!.!9!#, out. %&!% !inimum period to assess the potential of germination of coffee seeds8
Gabriel #astanheira Gui$ar2es1, Sttela ;ellyzette Feiga Franco da Rosa,C, Buis Fili%e Sera i$ #oelho1, (driano ;elly Feiga1, (line da #onsolaJ2o Sa$%aio #le$ente1

A(STRA$T @ 'n recent research, early stages o co ee seedling develo%$ent 8ere identi ied, a ter radicle %rotrusion, in 8hich all the essential %arts o the seedling can be assessed to de$onstrate the %otential o seed ger$ination to generate nor$al %lants7 Thus, the obLective o this research 8as to develo% studies to reduce the ti$e on the ger$ination test7 Ten seed lots o co ee 8ere assessed through the ger$ination test, every t8o days, ro$ the tenth day o so8ing, according to the $or%hological criteria o the seedlings7 The data 8ere co$%ared to the standard ger$ination test and analyzed by $eans o regression analysis and seg$ented nonlinear regression and %lanteau res%onse $odel7 't 8as concluded that the assess$ent o co ee seedlings at +0 days, in stage S+, %rovides the si$ilar result o the %attern ger$ination test, allo8ing a signi icant reduction o ti$e in order to de$onstrate the ger$ination o the seed lotsD the seedling assess$ent co ee in stage S1 does not have reduction o the ti$e or assessing the co ee ger$ination, in relation to the %attern7 7ndex ter s/ Coffea arabica, ger ination test, seg ented nonlinear regression, threshold, response plateau odel. (o)rna of Seed Science, -.29, n.2, p.2"#.29%, %&!2 8$RIO'R"S"RAA12O D" "I=OS "!(RIO0BRIOS D" $A34 *Coffea arabica +,'zul$G R7 '7 S(NT3S, MPrian T7 S7 !'R(, (ntonieta N7 S(B3Mj3, RosRngela #7 M"N;'M !$bra%a Recursos GenGticos e Biotecnologia, #) -1,61, #!) 6-.<5/56-, BrasPlia/;F, Brasil7 !$ailK izul$eNcenargen7e$bra%a7br

A(STRA$T: The e ect o 8ater content on the survival o e$bryonic a4es o Coffea arabica to reezing in liAuid nitrogen 8as investigated as %art o the e ort to develo% a cryo%reservation %rotocol or this s%ecies7 (4es 8ere e4cised ro$ seeds at di erent 8ater contents, both rozen in liAuid nitrogen or non rozen controls7 The viability o a4es e4cised ro$ control non/ rozen seeds 8as +--S, regardless o their initial 8ater content7 (4es e4hibited uni or$ dar9 red colour ollo8ing TTI test, suggesting the tissues 8ere viable and intact7 'n contrast, the initial 8ater content o cryo%reserved seeds deter$ined the viability o e4cised a4es7 "% to .0S o the a4es e4cised ro$ seeds containing +170S o 8ater re$ained viable a ter cryo%reservation7 ( ter the TTI test these a4es sho8ed bright red coloration throughout7 Vo8ever, only 6S o the a4es isolated ro$ seeds containing 157<S $oisture content 8ere viable a ter reezing in liAuid nitrogen7 Most o these a4es sho8ed large 8hite areas, an indication that tissues 8ere 9illed during the reezing>tha8ing %rocedure7 The cryo%reservation %rocedure described here is si$%le, does not reAuire the use o che$ical cryo%rotectants or e4%ensive %rogra$$able reezers, and $ost i$%ortantly yields high survival %ercentages7 The results obtained suggest that cryo%reservation o e$bryonic a4es is a good alternative or ger$%las$ conservation o co ee7 Si p>sio de ,esquisa dos Caf1s do Brasil $!./ %&&& / ,o@os de Caldas, 6?'

COR+DCID" /S" O3 6"R!"TI$ STORAG" 3OR T6" 'R"S"RAATIO0 O3 AGRI$/+T/RA+ 'ROD/$TS **%$BRID+ED ,ERSIO%To be presented at the 5th 'NT!RN(T'3N(B #3NTR3BB!; (TM3S)V!R! i F"M'G(T'3N #3NF!R!N#! &#(F* AntalyaD TurEeyD October 8F#8GD HI8H T7 ;e Bruin+, )7 Fillers1, (7 hagh,, S7 Navarro< +)resident, Grain)ro )hili%%ines, 'nc7, Subic Bay Free%ort Ione 1111, )hili%%ines &%hK k0,/<6/1:1/6..<*C 1)resident, Grain)ro, 'nc7, 1-- Ba9er (venue, Suite ,-5, #oncord, M( -+6<1, "S( &%hK 56./,6+/6++.* !$ailK %villersNgrain%ro7co$, hebsiteK 8887grain%ro7co$ ,(sia #ontinent Manager, <0, Wa$al Sagar R#F #olony, Bhandu%, !ast Mu$bai, 'ndia, !$ailK avinashNgrain%ro7co$ <Food Technology 'nternational #onsultancy Btd7, Beit Yohoshua <-:-+, 'srael

A(STRA$T: 'n 1-+1, the use o ully her$etic storage and solar drying to %reserve dried co$$odities such as grains, seeds and high value co$$odities, including co ee and cocoa, is no8 %racticed in .- countries7 Ver$etic storage has %roven es%ecially e ective in hot, hu$id cli$ates, as it co$bats reAuent and devastating %ost/harvest losses7 Ver$etic storage has also %roven e ective in %reventing the gro8th o $old/ %roducing a lato4ins, a $aLor %ublic health hazard7 This %a%er discusses the current state o the art, its li$itations and the various or$s that her$etic storage currently ta9es in order to $eet the needs o all ive continents7 The $ost %o%ular a%%lication o her$etic storage is the %reservation o $aLor grains, such as rice, corn, beans and 8heat, as 8ell as seeds and no8 silage, 8ithout re rigeration or che$icals7 Iey Jords/ Ker etic Storage, 6odified At osphere, Long Ter Storage, 7nsect Control, Flexible Storage Structures, Ker etic BunLerM, CocoonM, Super?rainbagM, TranSafelinerM, Solar 3ryer, SilbagM, ?rainSafeM, Collapsible 3ryer CaseM, ?rainIeep CenterM Arma%enamento de sementes de caf
-asa.o /oma Bra0"ini e 1)i2 Car os 3a2)o i Instituto AgronJmicoD $entro de $af KAlcides $arvalhoL * *8G- MHN8#F8OO ramal MPIQ MH8H#INFO R maEoSiac,sp,gov,br

(s se$entes de ca G, e$ condiJces nor$ais de ar$azena$ento &saco de %a%el, te$%eratura a$biente e u$idade de +:S das se$entes*, %erde$ a sua viabilidade ra%ida$ente7 Se$entes das duas es%Gcies econo$ica$ente $ais i$%ortantes, Coffea arabica e C. canephora, geral$ente %erde$ a ca%acidade de ger$inaJ2o a%s trOs a seis $eses da colheita7 )or esta raz2o, as %rHticas nor$ais de se$eadura s2o realizadas logo a%s a colheita ou no $H4i$o dentro do %razo de seis $eses7 ;esta or$a, no '(#, ora$ realizados vHrios estudos obLetivando in or$ar as $elhores condiJces %ara o ar$azena$ento de se$entes de ca G arHbica e robusta, de $odo a %reservar, %elo $aior %erPodo de te$%o %ossPvel, o $Pni$o de 6-S de ger$inaJ2o %ara ca G arHbica e 0-S %ara robusta, segundo as Nor$as e )adrces de )roduJ2o de Se$entes Fiscalizadas de #a G %ara o !stado de S2o )aulo7 )ara a conservaJ2o de se$entes de ca G das es%Gcies C. ar0bica e C. canephora, a $elhor condiJ2o %ara o ar$azena$ento G a do BaE rigorP ico co$ te$%eratura entre +- e +0T#, utilizando e$balage$ de saco de %olietileno de -,+0 $$ de es%essura e alta u$idade inicial de se$entes, ao redor de ,:S7 C AgronN ico, Ca pinas, 9($!', %&&#

$O0$+/S2O 3 ar$azena$ento de se$entes de ca G se a%resenta co$o u$ desa io %ara %rodutores de se$entes7 ( se$ente de ca G G classi icada co$o recalcitrante di icultando seu ar$azena$ento %or longos %erPodos de te$%o7 !ntretanto algu$as %esAuisas indica$ a necessidade de u$a nova classi icaJ2o7 ;entre os artigos %esAuisados %ode$os citar co$o $Gtodos %ara o ar$azena$entoK e$balagens her$Gticas, e$balagens e$ saco de %olietileno e$ baE rigorP ico e a crio%reservaJ2o do ei4o e$brionHrio7 (s %esAuisas direciona$/se %rinci%al$ente %ara a avaliaJ2o das $udanJas Psico/AuP$icas Aue ocorre$ nas se$entes durante di erentes ti%os de ar$azena$ento, o uso de %ri$ing e$ $atriz slida buscando a $elhoria da Aualidade isiolgica, a %ro%osiJ2o de testes co$ res%ostas $ais rH%idas %ara a avaliaJ2o das se$entes e a classi icaJ2o genGtica %ara ar$azena$ento e$ bancos de Ger$o%las$a7 ;evido d diversidade dos obLetivos dos trabalhos co$ se$entes de ca G observa/ se u$ vasto ca$%o %ara %esAuisa7 3s resultados %ara %esAuisas co$ en oAue no ar$azena$ento ta$bG$ diverge$ entre si, ortalecendo a necessidade de $ais estudos nesta Hrea7 )ode$os citar co$o %alavras/chave %ara %r4i$as %esAuisasK te$%eratura, u$idade, viabilidade, vigor e ger$inaJ2o7

/0IA"RSIDAD" 3"D"RA+ D" +AARAS Depto de Agricultura @ Setor de Sementes

(RM(I!N(M!NT3 ;! S!M!NT!S ;! #(Fl

)ro essoraK ;ra7 Maria Baene Moreira de #arvalho ;iscenteK Mariana de 3liveira e Silva

+AARAS 0OA"!(RO D" HI8M