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1,3,7-trimethylxanthine (caffeine)
3160
Mail: cwlin@tmu.edu.tw
structure of tannins :
Na2CO3
No reaction, so
caffeine remains
CH2Cl2 soluble
Washing:
a. 5% NaHCO3, b. Brine
Emulsion
Experimental Procedure:
place 6.0g anhydrous Na2CO3 in a beaker
Materials:
1.Tea leaves
2.Cheese clothes
3.Anhydrous Na2CO3
4.CH2Cl2
5.Brine
6.Anhydrous MgSO4
Caffeine/BS
CH2Cl2
layer 1
Caffeine/BS
Caffeine/BS
CH2Cl2
layer 3
BS
Discard
R. B. flask,
1. Extraction
Basic Techniques
1. Extraction
2. Filtration
3. Using a rotary evaporator
Steps in extraction
1. Preparation of separatory funnel
2. Adding the liquid
3. Mixing the layers
4. Separating the layers
5. Cleaning the separatory funnel
2
Set the s. f. in
an iron ring
2. Filtration:
a. Gravity filtration (simple filtration)
1. Select a round-bottomed
flask: half-full or
less with the solution
2. Connect the flask to the
rotary evaporator with
a joint clip
13
12
1. Power on