Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.

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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have? Your answer : c. increased diffusion rate Predict Question 2: What do you think will be the pressure result of the current experimental conditions? Your answer : c. pressure above the left beaker Stop & Think Questions: Why do you think there is no pressure change? You correctly answered: b. Sodium is able to diffuse through the pores. Why do you think there was no pressure change? You correctly answered: c. There is no net movement of water because the solute concentrations are the same. Experiment Data: Run Number 1 2 3 4 5 6 7 Solute Na+ ClNa+ ClNa+ ClGlucose Glucose Glucose Albumin Glucose MWCO 20 20 50 100 100 200 200 200 Start Conc. L 5.00 10.00 10.00 8.00 8.00 8.00 9.00 0.00 Pres. L 170 340 0 136 0 0 153 153 Start Conc. R 0.00 0.00 0.00 0.00 8.00 0.00 0.00 10.00 Pres. R 0 0 0 0 0 0 0 0 Rate 0.0000 0.0000 0.0167 0.0000 0.0000 0.0036 0.0000 0.0044

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Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will You correctly answered: c. double. 2. Which membrane did not allow Na+ Cl- to pass through? You correctly answered: a. 20 MWCO 3. When a solute is able to diffuse through a membrane You correctly answered: b. equilibrium is reached. 4. Water diffuses You correctly answered: c. toward solutes.

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Review Sheet Results 1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? Your answer: Increasing the NaCL concentration increased the pressure in the beaker. The reason why is because the water diffuses with its concentration gradient. I did not predict correctly. 2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different. Your answer: One way osmosis is similar to simple diffusion is that it is passive transport from a high to low concentration. Osmosis is different from simple diffusion in that osmosis uses aquaporins(selectively permeable membrane) as a means of transporting solvents. 3. Solutes are sometimes measured in milliosmoles. Explain the statement, Water chases milliosmoles. Your answer: As the solute concentration increases, the concentration of water is decresed. 4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction? Your answer: The 10mM clucose was able to diffuse but the 9mM albumin did not diffuse. The osmostic pressure on the Left side rose to 153mmHg whereas the right did not get any pressure. My prediction is correct

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