Effect of Momordica charantia on the Glucose Tolerance in Maturity Onset Diabetes

Author: J. Welihindaa, E.H. Karunanaya ea, M.H.!. "heriffb, K.".A. Jayasin#heb Ty$e of %ublication: Clinical Date of %ublication: 1986 %ublication: Journal of Ethnopharmacology. 17:277-282 (1986) Or#ani&ation's: D partm nt! of a"ioch mi!try an# $% #icin & 'aculty of % #icin & (ni) r!ity of Colom$o& *yn! y +oa#& Colom$o 8 (,ri -an.a).

(ntroduction /lthough !u$!tantial 0or. ha! $ n #on on th hypoglyc mic acti)ity of M. charantia on normal an# #ia$ tic la$oratory animal! (+i) ra& 19122 ,harma t al.& 19632 /.htar t al.& 1981) r lati) ly f 0 r port! ar a)aila$l on it! acti)ity n human!. / po!!i$l !yn rg !tic int raction in a pati nt $ t0 n chlorpropami# an# a curry ma# from M. charaqntia an# garlic ha! $ n pr )iou!ly r port # (/!lam an# ,toc.l y& 1979). /n impro) m nt in th tol ranc of an 4t rnal loa# of gluco! $y non-in!ulin # p n# nt #ia$ tic! ha! $ n o$! r) # $y - ath r#al t al. (1981) aft r an oral a#mini!tration of 53 ml of M. charantia fruit 6uic . 7h !am author! o$! r) # a !imilar impro) m nt aft r a #aily inta. of 3.28 .g of fri # M. charantia for 8-11 0 .!. / !imilar o$! r)ation ha! $ n ma# $y /.htar (1982) in pati nt! 7 #ay! aft r oral ing !tion (53 mg9.g $o#y 0t) of #ri # M. charantia po0# r. 'urth rmor & an in!ulin li. prot in call # :plant in!ulin; (*hanna ta al.& 1981) i!olat # from M. charantia ha! $ n !ho0n to po!! !! hypoglyc mic prop rti ! 0h n in6 ct # !u$cutan ou!ly ("al#0a t al.& 1977). < ha) pr )iou!ly r port # (*arunanaya. t al.& 1981) !ignificant oral hypoglyc mic acti)ity of M. charantia (that i! locally culti)at # an# u! # in th tr atm nt of #ia$ t !) a)aila$l in ,ri -an.a in la$oratory animal!. 7h pr ! nt !tu#i ! 0 r un# rta. n to furth r )aluat th oral hypoglyc mic acti)ity of ,ri -an.an )ari ty of M. charantia in non-in!ulin # p n# nt maturity on! t #ia$ tic!. Materials and )ethods Diabetic patients Eight n n 0ly #iagno! maturity on! t #ia$ tic pati nt! (a) rag ag mi!!ing t 4t2 y ar!) fr from . toaci#o!i!& c r $ro)a!cular #i! a! & acut myocar#ial infraction an# r nal complication! 0 r ! l ct #. 7h ! pati nt! 0 r not on any anti-#ia$ tic #rug!. Preparation of juice 'r !h unrip fruit! of M. charantia 0 r o$tain # from th mar. t& 0a!h # 0 ll an# th ! # r mo) #. 7h fl !hy part 0a! th n cut into thin !lic ! an# mac rat # n a m chanical grin# r. 7h pulp 0a! th n !=u > # in thic. cloth an# th 6uic o$tain # imm #iat ly fro> n. " for u!ing& th fro> n 6uic 0a! tha0 # an# c ntrifug at 13&333 r ).9min in a Damon mi!!ing t 4t.C. "-23/ c ntrifug at 1?C for 13 min. 7h

!up rnatant 0a! # cant # an# 133-ml ali=uot! of th cl ar 6uic 0a! gi) n orally to pati nt!. Oral glucose tolerance test 'ollo0ing an o) rnight fa!t& !tan#ar# gluco! tol ranc t !t (53g gluco! ) 0a! p rform # 0ith all pati nt! r c i)ing 133 ml #i!till # 0at r in!t a# of M. charantia 83 min $ for th gluco! loa#. 7h oral gluco! tol ranc t !t 0a! r p at # on a !u$! =u nt #ay 0ith all pati nt! r c i)ing 133 ml M. charantia 6uic 83 min $ for th oral gluco! loa#. Blood sugar estimation "loo# !ampl ! (3.35 ml) 0 r o$tain # $y fing r tip punctur u!ing micro cap! (Drummon# ,ci ntific Cmpany& (,/) an# th $loo# gluco! l ) l! 0 r !timat # $y th gluco! -o4i#a! m tho# of @ugg t an# Ai4on (1957). !esults and Discussion 7h gluco! tol ranc cur$ ! of all 18 pati nt! ar illu!trat # in 'ig. 1 0ith normal gluco! tol ranc cur) of a h althy a#ult for compari!on. Bt i! appar nt that all 18 pati nt! 0 r cl arly #ia$ tic. Comparati) analy!i! of th p rc ntag incr a! of $loo# !ugar of ach of th ! cur) ! 0ith th corr !pon#ing on ! o$tain # 0ith th a#mini!tration of M. charantia 6uic 83 min. Crior to gluco! loa#ing r ) al # that th pat nt! coul# $ #i)i# # into t0o group!. 7h fir!t group& con!i!ting of mi!!ing t 4tpati nt!& !ho0 # a !ignificant impro) m nt in gluco! tol ranc follo0ing th prior a#mini!tration of M. charantia 6uic . 7h ! con# group& con!i!ting of 5 pati nt!& !ho0 # no !ignificant impro) m nt in th ir gluco! tol ranc . Bn 'ig. 2& th m an (m an of th p rc ntag incr a! of $loo# gluco! D ,.E.%.) gluco! tol ranc cur) of th r !pon#ing group i! compar # 0ith that o$tain # 0ith prior a#mini!tration of M. charantia 6uic . ,tati!tical analy!i! $y ,tu# nt t-t !t r ) al # a !ignificant #iff r nc $ t0 n th t0o cur) ! from th 1.5 tim point (3.5 E C E 3.1& 3.5 E C E 3.1& 3.32 E C E 3.31& C F 3.331&C F 3.331 at 3.5& 1& 1.5& 2 an# 2.5 h& r !p cti) ly). ,imilar cur) ! for th group !ho0ing no impro) m nt in th gluco! tol ranc ar (mi!!ing t 4t) in 'ig. 8. 7h r 0a! no !tati!tical #iff r nc $ t0 n th t0o cur) !. @o0 ) r& th analy!i! of th total #ata& irr !p cti) of th appar nt #iff r nc in r !pon! to tr atm nt& !till confirm # th oral hypoglyca mic acti)ity of M.charanta. 7h m an total ar a un# r th t !t (M. charantia mi!!ing t 4t) gluco! tol ranc cur) ! (187.3 cm2) 0a! !ignificantly lo0 r (3.31 E mi!!ing t 4t E 3.331) than th ar a (218.6 cm2) un# r th control gluco! tol ranc cur) ! (7a$l 1). 7h #iff r nc in th r !pon! o$! r) # 0ith th fi) pati nt! in thi! !tu#y coul# al!o ha) $ n #u to intra-in#i)i#ual )ariation u!ually ! n in $iological !y!t m!. 7h pr ! nt !tu#y 0hil confirming th oral hypoglyca mic acti)ity of M. charantia gro0n in ,ri -an.a& gi) ! !ci ntific )ali#ity for th u! of M. charantia a! an oral hypoglyca mic ag nt $y th tra#itional m #ical practition r! of ,ri -an.a. Ac no*led#e)ents 7hi! 0or. 0a! !upport # $y grant Ao. 581 from th Bnt rnational 'oun#ation for ,ci nc & ,0 # n& an# $y th grant Ao. +G 88929 from th Aatural + !ourc !& ,ci nc an# En rgy /uthority of ,ri -an.a.

!eferences /.htar& %.,.& /thar& %./. an# Ha=u$& %. (1981). Eff ct of Momordica charantia on $loo# gluco! l ) l! of normal an# /llo4an #ia$ tic ra$$it!. Clanta % #ica 12& (8)& 235-212. /.htar& %.,. (1982) 7rial of Momordica charantia Linn. (.ar la) po0# r in pati nt! 0ith maturity on! t #ia$ t !. Journal of Ca.i!tan % #ical /!!ociation 82& 136-137. /!lam& %. an# ,toc.l y& J.@. (1979) Bnt raction $ t0 (Chlorpropami# ). -anc t B& 637. n curry ingr #i nt! an# #rug

"al#0a& I.,.& "han#ari& C.%.& Cangria& /. an# Goyal& +.*. (1977) Clinical trial 0ith pati nt! 0ith Dia$ t ! m llitu! on an in!ulin li. compoun# o$tain # from a plant !ourc . (pp!ala Journal of % #ical ,ci nc 82& 89-11. @ugg t& /.& ,t. G. an# Ai4on& D./. (1957) (! of gluco! o4i#a! & p ro4i#a! an# #iani!i#in in th # t rmination of $loo# an# urinary gluco! . -anc t ii& 868-873. *arunanaya. & E.@.& <lihin#a& J.& ,irimann & ,.+. an# ,inna#orai& G. (1981) Jral hypoglyca mic acti)ity of !om m #icinal plant! of ,ri -an.a. Journal of Ethnopharmacology 11& 228-281. *hanna& C.& Jain& ,.C.& Cangria& /. an# Di4it& I.C. (1981) @ypoglyca mic acti)ity of polyp pti# p from th plant !ourc . Journal of Aatural Cro#uct! 11& 618-655. - ath r#al & "./.& Can !ar& +.*. t al. Bmpro) m nt in gluco! tol ranc #u to M. charantia (.ar la). "%J 282& 1828-18212 +i) ra& G. (1912) Cr liminary ch mical an# pharmacological !tu#i ! on :Cu# amor; Momordica charantia L. Cart 2. /m rican Journal of Charmacology 1111& 72-87. ,harma& I.A.& ,ogani& +.*. an# /rora& +.". (1963) ,om o$! r)ation! on th hypoglyca mic acti)ity of Momordica charantia. Bn#ian Journal of % #ical + ! arch 18& 171-177.