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Inggrid Surono
Inggrid Surono
5/26/2009
Inggrid Surono
Function of Indigenous probiotic from dadih Enterococcus faecium IS-27526 in improving nutritional status and suppress diarrhoea
5/26/2009
Enterococcus faecium IS IS-27526 strain isolated from Dadih possess probiotic properties
Probiotic Properties
colonization Immuno-modulatory Antagonisms against pathogens antimutagenic, hypocholesterolemic Safety in food, clinical safety processing, shelf life, probiotics stability
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1500 million
episode of diare children younger than 5
Every 3 seconds 1 child dies from diarrhoea 70% cases related to contaminated food and drink
Other 35%
ARI 23%
Typhoid 11%
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Effect of supplementation fish protein concentrate biscuit with probiotic cream on response immune humoral of children younger than five
1. Imunoglobulin A (IgA) IgA of three groups before and after intervention
Treatment Before Normal biscuit Fish Protein Concentrate (FPC) Biscuit FPC + Probiotic cream 87.79 98.3 86.78 15.28 87.79 33.92
Serum Ig A After 91.74 12.04 95.01 17.94 107.61 38.21 Delta 3.95 3.28* 8.23 4.62** 19.83 16.35**
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Fecal Microbiota of children younger than five, before and after supplementation with FPC biscuit with probiotic cream
Profile of fecal microbiota 1 2 3 4 5 Fecal Aerobic bacteria (cfu/g) Log cfu/g Fecal Coliform (cfu/g) Log cfu/g Fecal lactic bacteria (cfu/g) Log cfu/g Fecal Anaerobic bacteria (cfu/g) Log cfu/g Fecal lactic Anaerobic (cfu/g) Log cfu/g
before 7.10 0.96 1.2 x 107 5.40 0.87 2.5 x 105 4.48 3.19 3.0 x 104 6.38 0.92 2.5 x 106 5.61 0.71 4.0 x 105
after 6.42 1.36 2.5 x 106 5.50 0.98 3.1 x 105 6.35 0.70 2.2 x 106 6.29 1.23 1.9 x 106 6.23 0.99 1.5 x 106
delta 0.96 1.65 0.07 1.28 1.86 3.41 0.09 1.15 0.62 0.45**
Number of subjects FPC biscuit + Probiotic cream (n=35) n 4 6 5 0 0 0 15 % 3.8 5.7 4.8 0 0 0 14.3
No.
Normal Biscuit (n=35) n % 5.7 3.8 7.6 2.9 5.7 3.8 29.5
1 2 3 4 5 6 Total
6 4 8 3 6 4 31
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Morbidity of children younger than five subjects during 90 days intervention study
Treatment Groups
Normal biscuit FPC biscuit FPC + Probiotic cream
Day 0
Diarrhoea High fever Common cold Dermatitis Fever Cough Measle Total 5 1 1 4 5 0 16
Day 90
1 0 3 2 1 1 0 8
Day 90
2 0 0 0 0 0 0 2
30
2 2 2 1 2 1 0 11
60
3 2 3 0 2 2 0 12
0
3 0 4 0 4 6 1 18
30
2 1 2 0 2 2 0 9
60
4 1 2 0 2 2 0 11
0
6 0 3 0 5 6 0 20
30
4 2 3 0 0 0 0 9
60
0 3 1 0 0 0 0 4
90
0 1 1 0 0 0 0 2
The improvement of Intestinal vili induces by probiotic LIS 10506 and LIS 20506 A: Normal Vili ; B: Disruption o of vili caused by LPS E.coli ; C: the improvement vili by LIS 10506 ; D: the improvement vili by LIS 20506
10
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Galectin-4
Myosin-1a
Occludin
ZO-1
Distribusi Ekspresi Protein Brush Border Usus Expression of intestinal brush border protein
30
p<0,05) p<0,05) p<0,05) p<0,05)
25 20 15 10 5 0 D J G I D
J M
J ZO
J O
11
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0 0 -0.5 30 60 90
Z sco re s
-1
-1.5
-1.59
Z skor -1.88
-2.03 -1.97 -2.02
(BB/U)
-2.01 -2.09
-1.79 -1.8
-2
-2.12 -2.23
-2.12
-2.5
Days
Biskuit Normal biscuit Biskuit KPI FPC biscuit Biskuit KPI+Probiotik FPC + probiotic cream
Z scores (weight/age)
Gambar 19
0 0 -0.5 30 60 90
Z scores
-1
-1.54 -1.59
-1.5 -2 -2.5
-1.72
-2.08 -2.35
-2.08
Days
Biskuit normal biscuit Biskuit KPI FPC biscuit Biskuit KPI+Probiotik FPC + probiotic cream
Z scores (height/age)
12
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0 -0.2 -0.4 0 30
-0.48
60
-0.56
90 -0.16
Z scores
-0.61
-0.93
-1.4 -1.6
Days
Biskuit Normal biscuit Biskuit KPI FPC biscuit Biskuit KPI+Probiotik FPC + probiotic cream
Z scores (weight/height)
Gambar 21
Note : weight/age: Normal -2<Z score<2 height/age: Normal : Z score -2 underweight -3<Z score<-2 Stunted : Z score < -2
13
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Randomised Placebo Control Trial, Pre-post study Two groups, n = 40 subjects, 90 days supplementation Probiotic E. faecium IS-27526 108 cfu/day
0.400
14
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(mg/ml)
0.052 0.050 0.004 0.000 no rmal -0.050 -0.1 00 underweight severe underweight -0.054 0.01 9
**
**
0.560 0.136
1.055
Probiotic
15
5/26/2009
1 .200 1 .000
1 .1 09
0.802 0.800
(mg/ml)
no rmal
underweight
severe underweight
Total sIgA (mg/ml) before and after intervention, based on the nutritional status of children less than five
11.6
placebo
11.2
probiotic
Weeks
16
5/26/2009
17