Plant Cytogenetic Experts

The workshop will involve two leading experts from the Molecular Cytogenetics Group at the University of Leicester, UK, who have jointly published over 300 peer reviewed articles in the field. This group specializes in research to understand large-scale processes of genome evolution, including chromosome rearrangement, genome control, recombinant, polyploidy, and epigenetics. Much of their research is focused on repetitive DNA, including tandem repeated satellites, DNA transposons and retrotransposons involving technology development work for in situ hybridization, photon imaging and genomic data analysis.

REGISTRATION FORM Name

FISH ChIP

&

IC number

workshop for plant genetic study

Gender

This unique workshop addresses the application of florescence in situ hybridization (FISH) and chromatin immunoprecipitation (ChIP) for studies with plants.

University/Institute/Company

John Seymour (Pat) Heslop-Harrison

Professor of Plant Cell Biology and Molecular Cytogenetics at the University of Leicester, UK. He is Chief Editor of Annals of Botany, and an accepting editor of Theoretical and Applied Genetics (TAG) and Plant Reproduction (formerly Sexual Plant Reproduction).

Email address

Mailing address

Trude Schwarzacher
She is a co-project leader of the Molecular Cytogenetics research group in the University of Leicester. She is a referee for many journals including Plant Journal, Chromosoma, Heredity, Theoretical and Applied Genetics, PNAS, Euphytica, Hereditas and Annals of Botany.

Phone number

All participants are advised to read about FISH and ChIP at webpages (http://www.molcyt.com and follow links) before coming to the workshop.
Please send registration form by email or fax to Fax: (603)7967 6991 Email: cebar@um.edu.my Registration closing date: 20th November 2013

When
17th December 2013 19th December 2013 Closing date for registration: 20th November 2013

FISH and ChIP

This three day workshop will include two main workshop sessions to cover use of each technique for analysis of plants.

Where
Malaysia Agro-biotechnology Institute, ABI Serdang. (Blok A Administrative building)

Registration fee
RM 1300 for each participant (Includes tea and lunch each day)

Workshops
In depth educational sessions will be given by experts covering FISH and ChIP techniques and applications. All participants will get hands-on experience directed by experts held with the purpose of demonstrating the techniques or processes for both FISH and ChIP. Participants work with and are exposed to equipment, specimens, chemicals and reagents.

FISH
FISH (Fluorescence in situ hybridization) has become an essential technique in molecular cytogenetics, involving the combination of molecular biology and cytogenetics. It can be used to detect and localize the presences or absence of specific DNA sequence on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence complementarity. Fluorescence microscopy can be used to locate where the fluorescent probe has bound to the chromosomes. FISH requires a combination of skills of molecular biology, cytogenetics, microscopy, cellular imaging analysis and immunocytochemistry. The workshop will provide effective and efficient details of protocols for FISH applied for plant cytogenetics.

ChIP
ChIP (Chromatin immunoprecipitation) allows specific proteinDNA interactions to be isolated. Combining ChIP with high-throughput sequencing (ChIPseq) reveals the DNA sequence involved in these interactions. The ChIP technique has been successfully used in many biological systems to study histone modifications of eu/heterochromatin and localization of regulatory chromatin factors. This method relies on the rapid cross-linking of protein/DNA complexes within the nucleus of living cells, followed by chromatin isolation, its random shearing and immunoprecipitation with antibodies directed towards proteins of interest. Levels of coimmunoprecipitated DNA are analyzed by PCR. The relative enrichment or depletion of a particular DNA fragment in the immunoprecipitated fraction reflects its in vivo association with the examined protein. The workshop will involve ChIP protocols for plant.

Lecture Series
Participants will gain a global perspective on plant science by attending the opening lecture on the 17th Dec 2013 morning session followed by specialized lecture and discussion sessions, embedded within the workshop for participants only.

Contact Us
Centre for Research in Biotechnology for Agriculture (CEBAR). Level 3, Institute of Research Management & Monitoring (IPPP), Research Management & Innovation Complex, University of Malaya, 50603 Kuala Lumpur. Tel: (6017)259 8211 Fax: (603)7967 6991 Email:cebar@um.edu.my Website: http://cebar.um.edu.my/

Co-organized by CEBAR University of Malaya, UM and Malaysia Agro-Biotechnology Institute, ABI