THE HYDERABAD ACADEMY

NIPER 1. Test: Biotechnology MaxMarks: 50 b) DNA Protein d) RNADNA Reverse transcription means a) DNA DNA c) DNA RNA

2.

The process of formation of m-RNA from DNA is called. a) Translocation b) Transcription c) Translation d) All Introduction of DNA into bacterial cell is called a) Translocation c) Translation

3.

b) Transformation d) None

4.

Enzyme required to cut the DNA at specific site at any where in the DNA other than the terminal region is a) Restriction Endo nucleases b) Exonuclease c) Both d) None SDS PAGE is used for. a) DNA c) Protein Antibody which can pass through the placenta a) Ig A b) Ig G c) Ig D d) Ig E Translation occurs in a) Nucleus c) Mitochondria Enzyme which require ATP for is activity is a) Ligase c) Methylase Proteins are polymeric structures of a) D-amino acids c) d-amino acids The factor required for initiation of transcription a) Sigma factor c) Protein factor 51-end of the DNA is where a) Phosphate is present c) Sulfate is present 1

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b) RNA d) All

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b) Ribosomes d) Cytoplasm.

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b) Nuclease d) Phasphatase

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b) L-amino acids d) All amino acids

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b) Rho factor d) Both a&b

11.

b) OH is present d) None

12.

Which of the following is not required for transcription? a) RNA polymerase b) Ribo nucleoside c) Ligase d) Rho factor Always the new DNA stand is synthesized in which direction a) 51 31 b) 3151 c) Any direction d) towards RNA 51AUG31 codes for a) Starting amino acid Valine b) Starting amino acid metheonine c) Terminal amino acid metheonine d) Terminal amino acid of protein chain Principle used in VDRL test is a) Agglutination c) Immuno fluroscence Principle used in WIDAL test is a) Agglutination c) Immuno fluroscence HIV is a a) DNA Virus c) Retro Virus D.P.T. is a) Bacterial Live Vaccine c) Toxoid of bacteria

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b) Precipitation d) ELISA

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b) Precipitation d) ELISA

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b) RNA Virus d) Myco plasma

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b) Viral Vaccine d) None

19.

Shick test is performed to ascertain Susceptibility to. a) Tetanus b) Mumps c) Syphilis d) Diphtheria Antiviral proteins are a) Interleukins c) Interdigitating cell Which one of the following is not a Vector. a) PBR 322 c) PUC 101 Vector which can replicate in two different hosts is a) Plasmid c) Shuttle Which of the following is not a stoping codon. a) UAA c) UGA

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b) Interferons d) None b) gt10 d) X174

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b) Cosmid d) YAC b) UGA d) UAC

23.

24.

Which one of the following is smallest RNA a) m-RNA c) t-RNA Delayed type of hypersensitivity is a) Anaphylaxis c) cell mediated Killed Vaccine is a) BCG c) polio Sabin Amplification of DNA is by a) PCR c) NMR

b)-RNA d) DNA

25.

b) Immune complex mediated d) type II

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b) polio Salk d) D.P.T.

27.

b) Transcription d) Blotting

28.

r-RNA present in 50s subunit of Prokaryotic ribosome is a) 16s r-RNA b) 23s r-RNA c) 18s r-RNA d) 5.8s r-RNA The coding sequences in DNA which code for amino acids are a) Introns b) Exons c) Both d) None The starting amino acid for prokaryotic protein synthesis is a) Valine b) Methionine c) Alanine d) N-Formyl methionine RNA directed DNA Polymerase is a) DNA polymerase c) Reverse transcriptase Which one of the following is linear vector is a) PBR322 c) Cosmid

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b) RNA polymerase d) None

32.

b) Bacteriophage d) Shuttle

33.

Type of mutation where a purine base is replaced by another purine base is called. a) Transition b) Transversion c) Translocation d) Transposones Which one of the following act as base analogue to Thymine a) 5-bromoUracil b) 5-chloro Uracil c) 5-Iodo Uracil d) All Nitrogen base which do not contain amine group a) Adenine c) Thymine

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35.

b) Cytosine d) Glucose

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49.

50.

Liposomes are a) Uni or) multilayered of phospholipids b) Enzymes c) Fibro peptides d) R.B.C. Man toux test is used for detection of a) Serum albumin b) Tuberculosis c) Polysaccharide antigen d) Laprosy Coombs test is also called a) Heme agglutination b) Rh typing c) VDRL d) WIDAL The enzyme used to relax the DNA or) reduce the torsinal strain produced on DNA because of high coiling is a) DNA polymerase b) RNA polymerase c) Topoisomerase d) Methylase Type of ribosomes present in Eukaryotes a) 80s b) 70s c) 50s d) 60s Chemical which can induce the mutation a) Nitrous acid b) Alkylating agents c) Mustard gas d) All Mutatiion caused by termination codons is a) Mis sense b) Silent c) Frame shift d) Spontaneous Western blotting is for a) DNA b) RNA c) Proteins d) Lipids Post transcriptional modifications are seen in a) Bacteria b) Virus c) Plant d) Cyanobacteria Which one of the following is functional unit of DNA a) Gene b) Ribosome c) Chromosome d) RNA Total number of codons present a) 20 b) 25 c) 61 d) 64 Factor in RNA polymerase help to bind the enzyme to DNA is a) b) 1 c) d) The fusion media used in hybridoma technology is a) poly ethylene glycol b) HAT media c) Agar media d) Mac Conkey agar C-DNA means a) Complex DNA b) Circular DNA c) Complementary DNA d) None Non Protein part of the enzyme is a) Apo enzyme b) Co enzyme c) Holo enzyme d) Conjugate enzyme

NIPER Biotechnology Key 1.d 2.b 3.b 4.a 5.c 6.b 15.b 16.a 17.c 18.c 19.d 20.b 28.b 29.b 30.d 31.d 32.b 33.a 41. d 42.a 43.c 44.c 45.a 46.d

7.d 21.d 34.d 47.c

8.a 22.c 35.c 48.a

9. b 23.d 36.a 49.c

10.a 11.a 12.c 14.b 24.b 25.c 26.b 27.a 37.b 38.b 39.c 40.a 50.b