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CM0463, Standard Plate Count Agar APHA | Oxoid - Product Detail

Dehydrated Culture Media

Sector: Industrial

STANDARD PLATE

You are viewing the printer friendly version of this page. To return to the regular view click here. COUNT AGAR (APHA) Code: CM0463 A standard medium corresponding to the APHA formulation for milk , water, food and dairy products.
Typical Formula* gm/litre Yeast extract 2.5 Pancreatic digest of casein 5.0 Glucose 1.0 Agar 15.0 pH 7.0 0.2 @ 25C

* Adjusted as required to meet performance standards

Directions Suspend 23.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into bottles and sterilise by autoclaving at 121C for 15 minutes. Description Standard Plate Count Agar was developed by Buchbinder et al.1 who wished to use an agar without milk solids in the formulation and investigated the control tests necessary to give standard results in dairy products with statistically valid counts. Standard Plate Count Agar meets the formulation of the APHA2, AOAC3 and PHLS4 . Storage conditions and Shelf life Store the dehydrated medium at 10-30C and use before the expiry date on the label. Store the prepared plates at 2-8C. Appearance Dehydrated medium: Straw coloured, free-flowing powder Prepared medium: Straw coloured gel Quality control
Positive control: Escherichia coli ATCC 25922 * Negative control: Uninoculated medium
* This organism is available as a Culti-Loop

Expected results Good growth; straw coloured colonies No change

Precautions When carrying out prescribed Standard Methods it is essential to follow the protocols given in precise detail. References 1. Buchbinder et al. (1951) Public Health Reports . 66. 327-329. 2. American Public Health Association (1976) Compendium of Methods for the Microbiological Examination of Foods. APHA Inc. Washington DC. 3. Association of Official Analytical Chemists (1995) Bacteriological Analytical Manual. 8th Edn. AOAC. Washington DC.
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CM0463, Standard Plate Count Agar APHA | Oxoid - Product Detail

4. PHLS Standard Methods for the Microbiological Examination of Food and Dairy Samples (1999) Aerobic plate count at 30C F10 & F11.
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