t0

Pp
Davis
Seth
Brad
Adriana
Skyla
Rika
Alicia
Scott
Rika
David
Mark
Rika
Tymon
Tatum
Fernando
Chris S
Chris H
Chris B
Sarah
Meg
Simone
Lara
Matthew
Mariah

1no
1no
1no
2no
2no
2no
3no
3no
3no
Cno
Cno
Cno
1Pp
1Pp
1Pp
2Pp
2Pp
2Pp
3Pp
3Pp
3Pp
CPp
CPp
CPp

0
0
0
0
0
0
0
0
0
0
0
0
7875000
45937500
0
21000000
44625000
30187500
0
0
157500000
0
105000000
6562500

t7
Pp

Other
Other
336000000
0
5788125000
20212500
0
420000000
63000000
0
6562500
1113000000
63000000
156450000
76125000
0
65625000
417375000
707000000
141750000
0
605062500
141750000
0
1211437500
141750000
0
819000000
60375000
9187500
360937500
129937500
0
2080312500
112000000
0
1246875000
459375000
26250000
496125000
308437500 294000000
513187500
150937500
0
265125000
22310000
36750000
26250000
7875000 570937500
290062500
24937500 360937500
19687500
57750000 639000000
532875000
65625000 948937500
190312500
958125000 4803750000
3294375000
31500000
0
34125000
31500000
0
0
231000000
21000000
307125000

For the missing data, take the average of the other two data points for that treatment and use that in any graphs y
This is not a good practice, however, and for future experiments you undertake, you would actually do another cou
Use the t21 data for your one-way ANOVA

t14
Pp
0
0
0
10500000
0
0
0
0
0
10500000
0
0
10500000
207375000
0
1211000000
1328750000
557812500
128875000
1694437500
1631475000
160125000
93187500
22312500

t21
Pp

Other
Other
674625000
0
862312500
362250000
0
636562500
1235062500
0 1157625000
2688000000
357000000 1995000000
42000000
0
162750000
690375000
0
719250000
677250000
0 1550062500
1143187500
0
693000000
174562500
0 1582875000
2502937500
3937500 3151312500
1960875000
0 3108000000
847875000
0
262500000
1557937
2625000
971250000
196875000
140437500
97125000
623437500
0
819000000
22310000 1406000000
23630000
82687500 1115625000
77437500
7875000
593250000
5250000
45937500 1552688000
2625000
107625000 1882125000
95812500
1351250000 14800000000
91875000
1057875000 2267500000 1370250000
2173500000
351750000 2807437500
513187500
86625000
958125000

t0
pp
1
2
3
4
5
6
7
8

0
0
0
0
17937500
31937500
52500000
37187500

t0
Pp
1no
2no
3no
Cno
1Pp
2Pp
3Pp
CPp

0
0
0
0
17937500
31937500
52500000
37187500

ment and use that in any graphs you create.

ou would actually do another count, which is why you preserve more than you need for just one count

t0
other
139737500
437062500
141750000
100770833.3
306250000
18374166.67
360500000
98000000

t7
pp
0
21000000
0
3062500
106750000
322875000
2130562500
7000000

t7
other
2071562500
383534375
878500000
1229375000
424812500
112000000
1339187500
113750000

t7
Pp

t14
Pp

t21
Pp

0
0
0
21000000
2625000 89250000
0
0
0
3062500
3500000
1312500
106750000 72625000 47687500
322875000 1032520833 1038291667
2130562500 1151595833 6078271000
7000000 91875000 901958333

t14
pp
0
2625000
0
3500000
72625000
1032520833
1151595833
91875000

t14
other
757312500
1024406250
665000000
1770562500
273956812.3
37624166.67
501604166.7
1248187500

1no
2no
3no
Cno
1Pp
2Pp
3Pp
CPp

t21
pp
0
89250000
0
1312500
47687500
1038291667
6078271000
901958333.3

t21
other
885500000
1106765625
1275312500
2173937500
629125000
35439166.67
63437500
1711937500

t0
Other
139737500
437062500
141750000
100770833.3
306250000
18374166.67
360500000
98000000

t7
Other
2071562500
383534375
878500000
1229375000
424812500
112000000
1339187500
113750000

t14
Other
757312500
1024406250
665000000
1770562500
273956812.3
37624166.67
501604166.7
1248187500

t21
Other
885500000
1106765625
1275312500
2173937500
629125000
35439166.67
63437500
1711937500

1711937500

901958333.3

63437500

35439166.67

1038291667

47687500

629125000

t21 Other

2173937500
1312500

1275312500
0

1106765625

89250000

885500000

CONCENTRATIONS

t21 Pp

6078271000

CONCENRATIONS OF P. PARVUM AND OTHER

PHYTOPLANKTON AT T21

1312500
CNO

1PP

63437500

0
3NO

35439166.67

89250000
2NO

47687500

0
1NO

2PP

3PP

CPP

TREATMENTS

Average cell densities per week of other phytoplankton

AVERAGE CELL DENSITY

2.5E+09

2E+09

1.5E+09

1E+09

500000000

0
Other

Other

Other

Other

t0

t7

t14

t21

WEEK
1no

2no

3no

Cno

1Pp

2Pp

3Pp

CPp

Average cell densities per week of P. parvum

7E+09

AVERAGE CELL DENSITY

6E+09
5E+09
4E+09
3E+09
2E+09
1E+09
0
Pp

Pp

Pp

Pp

t0

t7

t14

t21

WEEK
1no

2no

3no

Cno

1Pp

2Pp

3Pp

CPp

Treatment

Replication
1
1
1
2
2
2
3
3
3
4
4
4
5
5
5
6
6
6
7
7
7
8
8
8

t0
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3

Treatment
1no
2no
3no
Cno
1Pp
2Pp
3Pp
CPp

t7

t14

t21

2.69
2.93
2.69
3.08
2.84
2.9
3.05
3.01
2.98
2.75
2.79
2.92
5.04
5
4.42
4.96
4.92
4.82
4.62
4.73
4.69
4.8
4.65
4.62

12.3
4.75
8.7
9
5.62
9.85
12.9
8.73
15.6
12.6
8.1
15.9
11.1
10.1
12.8
14
8.75
17
19.2
17.9
15.9
3.87
13
8

30.3
8.27
11.1
7.83
7.93
89.7
9.78
5.8
10
46.1
23.2
6.03
27.1
20.2
23.5
44.1
35.1
39.5
46.4
42.6
45.3
24.9
24.6
27.2

2.77
2.94
3.013333
2.82
4.82
4.9
4.68
4.69

t7
8.583333
8.156667
12.41
12.2
11.33333
13.25
17.66667
8.29

t14
16.55667
35.15333
8.526667
25.11
23.6
39.56667
44.76667
25.56667

t0

12.4
26.4
13.1
14.3
16
8.02
13.2
11.5
5.3
59.8
26.8
11
24.8
7.46
27
40.7
49.1
38.1
67.3
63.4
59.5
42.9
30.5
50

t21
17.3
12.77333
10
32.53333
19.75333
42.63333
63.4
41.13333

Treatment ID
1no
1no
1no
2no
2no
2no
3no
3no
3no
Cno
Cno
Cno
1Pp
1Pp
1Pp
2Pp
2Pp
2Pp
3Pp
3Pp
3Pp
CPp
CPp
CPp

davis
brad
seth
skyla
rika
adriana
alicia
rika
scott
david
mark
rika
tymon
tatum
fernando
chris b.
chris h.
chris s.
meg
simone
sarah
lara
mariah
matthew

ETRY READINGS EACH WEEK

Average Fluorometry data

70
60
50

63.4

AVERAGE FLUOROMETRY READINGS EACH WEEK

50
42.63333333
39.56666667

40
35.15333333

32.53333333

30

25.11
20

12.77333333 12.41
12.2
10
8.583333333 8.156666667 8.526666667
3.013333333 2.82
2.94
2.77

0
1no

2no

3no

Cno

11.33333333

1Pp

t7

t14

2Pp

t21

17.66666667
13.25
4.9

4.82

TREATMENTS
t0

25.56666667

23.6
19.75333333

17.3
16.55666667

10

44.76666667
41.13333333

4.68
3Pp

CPp

44.76666667
41.13333333

25.56666667
17.66666667
8.29
4.69
CPp

Final cell densities (t21)

Pp
1ppt (ave)
2ppt (ave)
3ppt (ave)
0ppt (ave)

629125000
35439166.67
63437500
1711937500

Setting up the data as below allows comparisons of the treatments (group

0ppt
"Other" phyto in non-Pp flasks

"Other" phyto in Pp flasks

3151312500
3108000000
262500000
1370250000
2807437500
958125000

Other
885500000
959000000
1275312500
2173937500

Although I told you that you would have to aver

program will average the individual groups (trea

omparisons of the treatments (groups) to each other

1ppt
862312500
636562500
1157625000
971250000
97125000
819000000

Your output should

2ppt
3ppt
1995000000
1550062500
162750000
693000000
719250000
1582875000
23630000
2625000
77437500
95812500
5250000
91875000

Source of Varia

t you would have to average the replicates in order to run the ANOVA, that is not correct because if you set up the data the w
he individual groups (treatments).

ur output should be similar to this:

Anova: Single Factor
SUMMARY
Groups
0ppt
1ppt
2ppt
3ppt
ANOVA
Source of Variation
Between Groups
Within Groups

Count
6
6
6
6
SS
7.83418E+18
1.41226E+19

Sum
11657625000
4543875000
2983317500
4016250000
df
3
20

Average
1942937500
757312500
497219583.3
669375000
MS
2.61139E+18
7.06131E+17

Variance
1.53732E+18
1.34256E+17
6.0941E+17
5.43537E+17
F

Total

2.19568E+19

23

ecause if you set up the data the way I have it below, excel's

P-value

F crit

0ppt
Other pyhto in Non pp flask

3151312500
3108000000
262500000
1370250000
2807437500
958125000

Other phyto in pp flask

1ppt
2ppt
862312500 1995000000
636562500 162750000
1157625000 719250000
971250000
23630000
97125000
77437500
819000000
5250000

Anova: Single Factor

SUMMARY
Groups
Column 1
Column 2
Column 3
Column 4

Count
6
6
6
6

ANOVA
Source of Variation
Between Groups
Within Groups

SS
7.4143E+18
1.3753E+19

Total

2.1167E+19

Sum
11657625000
4543875000
2983317500
4878562500

df
3
20
23

3ppt
1550062500
693000000
1582875000
2625000
958125000
91875000

Average
Variance
1942937500 1.53732E+18
757312500 1.34256E+17
497219583.3 6.0941E+17
813093750 4.69632E+17

MS
F
P-value
F crit
2.47145E+18 3.594022565 0.031674 3.098391
6.87654E+17

The experimental design of our lab experiment does not meet the criteria for a two
ANOVA, so please do not run it and report on it. Refer to the Statistics Instructions for
students document for instructions on another option to gain points.

If we had experimented with another factor, like light or temperature or nutrient levels,
see what its effect was on the growth of the phytoplankton, we could run a two

eet the criteria for a two-way

Statistics Instructions for

mperature or nutrient levels, to

, we could run a two-way ANOVA.