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Code No: R050211101 Set No.

1
II B.Tech I Semester Regular Examinations, November 2007
APPLIED CHEMISTRY AND BIOCHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) Explain the terms:


i. Specific Conductance and
ii. Equivalent conductance.
(b) Describe an experimental method to determine the equivalent conductance of
an electrolyte. [4+4+8]
2. (a) What are the properties of unplasticized PVC? What changes occur in it on
plasticization?
(b) How is Teflon manufactured? What are its applications? [8+8]
3. (a) What is a lubricant? How are lubricants classified? Give Examples.
(b) Give, with examples, the mechanism of
i. Hydrodynamic
ii. Boundary lubrication. [8+4+4]
4. (a) Define a living cell and draw a neat diagram of a plant cell along with the
organelles.
(b) How the fractionation of cell organelles is carried out by centrifugation method?
[8+8]
5. (a) How an enzyme is extracted and purified from a living cell?
(b) Define the unit of enzyme activity. How can you explain the purification of
an enzyme? [8+8]
6. Write a short note on:
(a) Transcription.
(b) Translation.
(c) Replication. [5+5+6]
7. (a) Describe the chemical composition of urine under normal conditions. Add a
note on tubular maximum ( Tm ).
(b) What are the normal and abnormal constituents of urine? Explain. [8+8]
8. What enzymes and metabolites are present in normal blood and add a note on
SGOT and SGPT. [16]

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Code No: R050211101 Set No. 2
II B.Tech I Semester Regular Examinations, November 2007
APPLIED CHEMISTRY AND BIOCHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define electrolyte and non-electrolyte with suitable examples.


(b) Define strong and weak electrolytes
(c) Explain Arrhenius ionic theory along with postulates. [5+5+6]

2. (a) What is the Composition of natural rubbers? How is it obtained from its
natural source?
(b) Write a note on vulcanization of rubber. [8+8]

3. (a) What is lubrication? What are the requirements of a good lubricant?


(b) Under what operating conditions would you use,
i. lubricating oils,
ii. Greases,
iii. Solid lubricants? Explain with examples. [4+4+4+4]

4. (a) Differentiate between prokaryotic and eukouyotic cells with suitable examples.
(b) Explain the composition or cell walls of prokaryotic cells. [8+8]

5. What is an enzyme? What is its chemical nature? Explain how the enzymes
accelerate reactions. [16]

6. Discuss the Nucleic acid chemistry with suitable examples. Write about Replica-
tion. [16]

7. (a) Illustrate the structure of hemoglobin.


(b) Describe biological functions and mechanism of hemoglobin. [8+8]

8. Write a short note on:

(a) Fluorometry
(b) Flame photometry. [8+8]

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Code No: R050211101 Set No. 3
II B.Tech I Semester Regular Examinations, November 2007
APPLIED CHEMISTRY AND BIOCHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define the terms , specific conductivity and molecular conductivity . what are
its units. What is electrolytic cell.
(b) Describe the westar standard cell with a neat diagram. [8+8]

2. (a) What is crepe rubber? How is it made?


(b) Explain the compounding and fabrication of rubber. [8+8]

3. (a) What is lubrication? What are the requirements of a good lubricant?


(b) Under what operating conditions would you use,
i. lubricating oils,
ii. Greases,
iii. Solid lubricants? Explain with examples. [4+4+4+4]

4. (a) What are prokaryotes? Give examples.


(b) Explain chemical composition of cell walls of prokaryotes.
(c) Explain how the membrane lipids play important role in membrane physical
phase transition under different temperatures. [4+6+6]

5. (a) Explain the kinetics of multisubstrate reactions.


(b) Explain the effect of temperature and PH as enzymic reactions. [8+8]

6. Define genetic code and discuss its characteristic features. Discuss about Sigma
factor and Replication fork. [16]

7. Write a short note on:

(a) Electrolyte status of the patients.


(b) Acid base balance.
(c) Zone Electrophoresis. [6+5+5]

8. Give an account of the general laboratory methods adopted for measuring any three
biochemical constituents of blood. [16]

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Code No: R050211101 Set No. 4
II B.Tech I Semester Regular Examinations, November 2007
APPLIED CHEMISTRY AND BIOCHEMISTRY
(Bio-Medical Engineering)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. what is polymerization ? Discuss different types of polymers. Define tacticity and


copolymerization. [16]

2. (a) What is novolac resin and how is it made?


(b) Describe the conversion of novolac resin to a cross-linked polymer. [8+8]

3. What are the boiler troubles? How the boiler feed water hardness is regulated for
steam pressures of different boilers? Explain the method to maintain the required
hardness of boiler feed water. [16]

4. (a) Differentiate between prokaryotic and eukouyotic cells with suitable examples.
(b) Explain the composition or cell walls of prokaryotic cells. [8+8]

5. (a) What are immobilized enzymes? Explain their role in industry.


(b) Define isoelectric point. How does it help in the extraction of enzyme from
solution?
(c) Give a general procedure for the extraction of an enzyme from tissues. [6+5+5]

6. Describe the replication of DNA. [16]

7. (a) What are ketone bodies?


(b) Explain how ketone bodies are formed.
(c) What is the clinical significance of ketone bodies formation? [5+6+5]

8. Discuss the principles and classification of Chromatography technique. [16]

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