Question 1 The light halo that is seen around cells viewed by phase contrast is caused by....

A) poor focussing on the specimen. B) the staining technique used to prepare the sample. C) phospholipids in the cell membrane. D) an artefact of the illumination. Question 2 1 / 1 point

1 / 1 point

Setting up a light microscope for phase contrast illumination requires... (check all answers that may apply) A) a special objective. B) a polarizer. C) a phase anulus. D) a phase condenser. Question 3 Differential Interference Contrast microscopy is also known as... A) dark field B) bright field C) phase D) Nomarski Question 4 Phase contrast and DIC both... A) visualize specimens that are alive. B) visualize specimens based on differences in refractive index. C) visualize specimens that have to be stained. D) visualize specimens that are easily seen under bright field. Question 5 1 / 1 point 1 / 1 point 1 / 1 point

From the video of preparing the Drosophila heart for TEM the uranyl acetate solution is used to.. A) dehydrate the sample.

B) fix the sample. C) prevent the sample from drying out. D) stain the sample. Question 6 1 / 1 point The resolution of electron microscopy is _______ light microscopy because electrons have a(n) _________ wavelength in comparison to light. A) the same as; equal B) less than; longer C) higher than; shorter D) less than; shorter E) higher than; longer Question 7 1 / 1 point

Which of the following is an advantage of using green fluorescent protein to monitor the localization of proteins of interest? A) It can be used to detect antibodies generated against a protein in the cell B) It increases the resolution of the specimen C) It can be used to observe dynamic processes occurring within the cell D) a and c E) all of the above Question 8 Which of the following is false regarding confocal scanning microscopy? A) Samples must be sectioned prior to visualization. B) A laser beam illuminates a single point and depth of the sample C) A composite image is displayed on a computer screen D) Digital images are recorded by a photomultiplier tube. E) It reduces fluorescence from above and below the focal point Question 9 Why is Transmission Electron Microscopy performed in a vacuum? A) To prevent oxidation of the sample B) To immobilize the specimen. 1 / 1 point 1 / 1 point

C) To maintain a constant temperature D) To retain moisture E) Because electrons can be dispersed by the air Question 10 1 / 1 point Which of the following is a similarity between transmission electron microscopy and scanning electron microscopy? A) Both have a limit of resolution of 0.1nm B) Both produce images of the 3D structure of the surface of a sample C) Both require that the cell be sectioned before staining D) Both use electrons that pass through the sample E) None of the above 1 / 1 point

Question 11 Choose the type of microscopy that is best suited to visualize each of the following:

__2__ __3__ __4__ __1__

Visualizing ribosomes within a cell Comparing cell types within a tissue sample Visualizing the three dimensional structure of the surface of a tissue sample Monitoring the localization of a protein in a cell

1. Fluorescence microscopy 2. Transmission Electron miscoscopy

3. Brightfield microscopy 4. Scanning Electron Microscopy 3 / 3 points

Question 12 Match the images below to the corresponding type of microscopy.

__6__ 1. Confocal 2. Nomarski 3. Brightfield 4. Phase Contrast 5. Scanning Electron Microscopy 6. Transmission Electron Microscopy __5__

__2__

__3__

__1__

__4__

Question 13

4 / 4 points

Identify the cellular organelles and structures indicated in this electron micrograph. ANSWER WITH THE NUMBER, NOT THE LETTER!!! 1. A 2. B 3. C 4. D 5. E 6. F 7. G 8. H

__1__ __7__ __8__ __5__ __6__ __3__ __2__ __4__

mitochondrion plasma membrane intercellular space Golgi endoplasmic reticulum nuclear membrane nucleus secretory vesicle

18 (100 Attempt Score: / %) 18 Overall Grade (highest attempt): 18 (100

/ %) 18
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