ChE513: Biochemical Engineering, AY2013-14 University of Santo Tomas En%r& A'hi((es Es)a(*on

Submission Date: 5ChE-A: June 28, 20 ! "#ri$ 5ChE-+: June 25, 20 ! "Tue$ 5ChE-C: June 25, 20 ! "Tue$ Assignment # 1

1. ,ate measurements -ere ma*e on a foo* en.yme -ith an* -ithout )resen'e of an inhibitor -ith the fo((o-in% resu(ts& /nhibitor 'on'entration is 0/1 2 20u3 A B 0S1, u3 v, u34min v, u34min 25 8&2 5&55 50 2&85 8& 5 65 5&5 7&58 00 6&75 &08 50 20&02 !&55 200 22&58 5&6 a. Confirm the ty)e of inhibition by )(ottin% a !r* set of *ata b. 9hat further tests are nee*e* to im)rove the a''ura'y of the estimate of : !; c. Determine :m an* <m for the en.yme -ithout inhibition as -e(( as :!, the inhibitor e=ui(ibrium 'onstant after ma>in% a**itiona( tests on b& 2. Derive the rate e=uation for ? formation usin% a quasi steady-state assumption use* in your bio'hemistry '(ass a year a%o& Assume stea*y-state for 0E&S1 an* 0ES&S1& E @ S
>m

E&S @ S
>s

>2

E @ ?

ES&S

>8

E&S @ ?

!& An eA)eriment on the therma( *e%ra*ation of an en.yme -as 'arrie* out an* some of the resu(ts are be(o-B Time, s CA'tivity D 55C CA'tivity D65C 0 00 00 00 72 85 200 85 62 500 56 85 000 85 20 a& ?(ot a %ra)h of (o% a'tivity vs& time an* *etermine the ina'tivation rate 'onstants at 55C an* 65C& b& Determine the a'tivation ener%y for the therma( *e%ra*ation of the en.yme&

4. An en.yme 'a((e* 'y'(o-oAy%enase "CEF$ is )ro*u'in% )rosta%(an*in -hi'h is res)onsib(e for )ain an* inf(ammation has a
:m of 8&6A 0-53& /f the <m of the )re)aration is 22 umo(4G&min,

a. -hat ve(o'ity -ou(* be observe* in the )resen'e of 2A 0-83 substrate an* 5A 0-83 ofB
i& Com)etitive inhibitor ii. Hon-'om)etitive inhibitor iii& Un'om)etitive inhibitor /f :! in a(( 'ases is !A 0-83

b. Iiven these JCEF inhibitorsK "another eAam)(e of -hi'h is /bu)rofen$, -hi'h one -ou(* you )refer to ta>e as
me*i'ine;

"I pledge on my honour that as the group representative, I have honestly accomplished this homework and assignment only with the help of my groupmates and that sharing our solutions, answers and ideas to other groups violates the principle of independent thinking and working which constitutes our core integrity as students of ChE !" Biochemical Engineering# I $elieve that doing my $est effort with the right mindset and attitude will fulfill my highest ideals in learning as a student of this pontifical, royal and catholic university#"

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