Académique Documents
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Methods
of
sterilization/disinfection:
a. flaming
till
red
hot
b. dipping
in
95%
ethanol
+
flaming
c. dry
heat
oven
d. moist
heat/autoclave/pressure
cooker
e. filtration
f. UV
irradiation
g. 70%
ethyl
alcohol/isopropyl
alcohol
Choose
from
the
list
above
(letter
only)
the
most
appropriate
method
of
sterilization/disinfection/decontamination
for
the
following
objects/substances
11. wireloop
12. empty
plates
13. flask
with
nutrient
agar
14. empty
glass
pipets
15. wireneedle
16. plates
with
agar
medium
17. laboratory
benchtop
18. enzymes
19. vitamins
20. human
skin
Complete
the
table.
Plating
technique
Type
of
growth
Material(s)/substance
(surface/subsurface/both)
used
to
separate
microorganisms
a.
streak
(21)
(22)
b.
spread
(24)
(25)
c.
pour
(27)
(28)
Volume
of
initial
inoculum
(max.
volume)
(23)
(26)
(29)