Vous êtes sur la page 1sur 2

1.

The appropriate pH indicator for pH 9.0 medium is


a. bromocresol green
b. bromocresol purple
c. bromothymol blue
d. phenolphthalein
2. The color of the medium (pH 9.0) with indicator before sterilization is
a. purple
b. pink
c. brown
d. colorless
3. The color of the medium (pH 9.0) with indicator after sterilization is
a. purple
b. pink
c. brown
d. colorless
4. The appropriate pH indicator for pH 7.0 medium is
a. bromocresol green
b. bromocresol purple
c. bromothymol blue
d. phenolphthalein
5. The color of the medium (pH 7.0) with indicator before sterilization is
a. blue
b. green
c. yellow
d. colorless
6. The color of the medium (pH 7.0) with indicator after sterilization is
a. blue
b. green
c. yellow
d. colorless
7. The appropriate pH indicator for pH 4.0 medium is
a. bromocresol green
b. bromocresol purple
c. bromothymol blue
d. phenolphthalein
8. The color of the medium (pH 4.0) with indicator before sterilization is
a. blue
b. light green
c. yellow
d. colorless
9. The color of the medium (pH 4.0) with indicator after sterilization is
a. blue
b. light green
c. yellow
d. colorless
10. Which of the following media had lost its gelling power after sterilization?
a. pH 9.0 coconut water agar
b. pH 7.0 coconut water agar
c. buffered pH 7.0 coconut water agar
d. pH 4.0 coconut water agar


Methods of sterilization/disinfection:
a. flaming till red hot
b. dipping in 95% ethanol + flaming
c. dry heat oven
d. moist heat/autoclave/pressure cooker
e. filtration
f. UV irradiation
g. 70% ethyl alcohol/isopropyl alcohol

Choose from the list above (letter only) the most appropriate method of
sterilization/disinfection/decontamination for the following objects/substances
11. wireloop
12. empty plates
13. flask with nutrient agar
14. empty glass pipets
15. wireneedle
16. plates with agar medium
17. laboratory benchtop
18. enzymes
19. vitamins
20. human skin

Complete the table.
Plating technique
Type of growth
Material(s)/substance
(surface/subsurface/both) used to separate
microorganisms
a. streak
(21)
(22)
b. spread
(24)
(25)
c. pour
(27)
(28)


Volume of initial
inoculum (max.
volume)
(23)
(26)
(29)

Vous aimerez peut-être aussi