AUF SOM DEPARTMENT OF BIOCHEMISTRY UNIT EXAM IN NUCLEIC ACIDS July 29, 2013 NAME ____________________________________________________________________ I. MULTIPLE CHOICE.

Please write the letter of the BEST ANSWER.

______1. The carbon atom of the sugar that is involved in the formation of the beta Nglycosidic bond in the sugar-base moiety in nucleic acids. A. Carbon 1 B. Carbon 2 C. Carbon 5 D. Carbon 3

______2. What type of bond is formed between a phosphate group and a sugar in the phosphate- sugar backbone? A. ionic bond B. electrostatic bond ______3. C. Hydrogen bond D. covalent

What type of bond is affected during the denaturation of Nucleic Acids? C. Non covalent D. Ionic bond

A. Hydrogen bond B. covalent bond

______4. What specific technique was used in the 1950s to identify the exact chemical structure of DNA? A. SDS PAGE B. X-ray crystallography ______5. C. Nuclear Magnetic Resonance D. Western blot

Which is a function of DNA?

A. carries the information to the site of protein synthesis B. carries the genetic code C. building blocks of proteins D. stores genetic information ______6. Which of the following major structures of DNA is left handed? B. B DNA D. C DNA

A. Z DNA C. A DNA ______7.

Which level of the DNA structure has a packing ratio of around 10? C. Solenoid D.A chromosome in metaphase

A. Bare double-helix DNA B. 10 nm fibril of nucleosome ______8. A. B. C. D.

It is the distance measured in one single turn of the DNA helix (B form) : 0.34 nm 34 nm 3.4 nm Distance would depend if measurement was done in the major or minor groove.

The base composition of DNA of an infant differs from an elderly. The “default” or the physiologic DNA seen in the cell C DNA B DNA A. True of Histone H1 EXCEPT: C. Not found in nucleosome core II. Rich in Arginine D. Occur naturally in regions of DNA that have a sequence of alternating purines and pyrimidines (e. Which statement best depicts the principles on DNA emphasized by the Chargaff’s rules? A. A DNA C DNA B DNA ______14. A DNA ______13. It is the “end product” of transcription. B. C. D. C.______9. The base composition of DNA generally is essentially the same among eukaryotes. ENUMERATION For Numbers 16 & 17 : Give at least (2) two differences between DNA and RNA : _____________________________________________________________________ _____________________________________________________________________ For Numbers 18 & 19 : Mention at least (2) differences between B DNA and A DNA: _____________________________________________________________________ _____________________________________________________________________ . A DNA ______12. : A. poly GC) A. ______10. B. D. C. B. There are “forces” or interactions that stabilizes the DNA duplex. Monocistronic mRNA codes for only one polypeptide and is usually seen in prokaryotes. Most tissue-specific and species-specific B. Found in linker DNA A. Z DNA B. It helps the ribosome by catalyzing the formation of peptide bonds during protein synthesis. D. Z DNA B. It transports amino acids to site of protein synthesis. A=G and C=T ______15. DNA isolated from the skin of humans has the same base composition as the ones isolated from the human liver. Z DNA B. These include The phosphodiester bonds N Beta glycosidic bonds between sugars and bases Hydrophobic interactions of successive base pairs peptide bonds contributed by histones Conformation of DNA found in DNA-RNA hybrids C DNA B DNA A.g. ______11. True of messenger RNA : A.

Adenine Bonus point : The DNA is a double helix. Write the name of the corresponding NUCLEOSIDE using the available bases below.” ― Joel Osteen . NOMENCLATURE For Numbers 22 to 25. Guanine 23.III. What structures form the rungs of the DNA ladder? Nucleoside ____________________________________________ ____________________________________________ ____________________________________________ ____________________________________________ “Certainly. but you have to understand that your struggle may be an opportunity for advancement and promotion. The very thing you are fighting against so tenaciously may be the springboard that catapults you to a new level of excellence. Base 22. others use the analogy of the ladder. Show specific carbons involved in the Phosphoester and glycosidic bonds. No need to draw the nitrogenous base . STRUCTURE (2 points) For Numbers 20 & 21 Illustrate : A Nucleotide /“The Perfect Trio”. Your are to synthesize a DNA molecule. Thymidine 25. Cytosine 24. none of us enjoy going through struggles. Your challenges may become your greatest assets.-) IV.