THE GASTROPROTECTIVE EFFECT OF SAMPAGUITA (J asminum sambac)
AGAINST INDOMETHACIN-INDUCED GASTRIC ULCER IN RATS
A Thesis Presented to the Department of Pharmacy College of Allied Medical Professions Angeles University Foundation
In Partial Fulfillment of the Requirements for the Degree BACHELOR OF SCIENCE IN PHARMACY
By: DIANA ROSE D. ANUSENCION PAULINE ANDRI S. AYSON GIZELLE ERIKA R. BUSTOS OLYSA NICARE C. MANDANI RICHELLE O. SANCHEZ
March 2014
ACKNOWLEDGEMENT
The researchers would like to extend their gratitude to the following persons for serving as their inspiration and guide throughout the span of the study: Mr. Rashlee Aibert Manalang, for always helping the researchers through guiding them with the written manuscript and never failed to give the researchers determination; Ms. Irene Jane Montoya and Ms. Rodella Salas, for all the understanding they have imparted and for guiding, sharing their expertise and suggestions and encouragement to the researchers and enough encouragement to finish the study; Dr. Estelita Cayabyab and the Faculty of Pharmacy, for giving the researchers advice as they consult about certain matter and for sharing their knowledge; Mr. Richard Ryan Mergal for understanding and allotting his time to guide the researchers in their statistical analysis and findings; The parents of the researchers, for showering them with affection, and love for supporting them mentally, emotionally and financially all throughout their study; The researchers colleagues, for their unending support; and Most importantly, to God Almighty, who gave the researchers guidance, strength, knowledge, determination, and perseverance throughout their lives especially in the duration of their study.
ABSTRACT
Jasminum sambac is popularly known in the Philippines as Sampaguita. In folkloric medicinals, J.sambac which is a flowering plant from the family Oleaceae, is used as treatment for several diseases. Its leaves are primarily used for fever, coughs and colds, lactifuge, sprains, fractures and ulcers. This study evaluated the gastroprotective effects of J. sambac leaves against non-steroidal anti-inflammatory drugs (NSAIDs) induced gastric ulcers in rats. Healthy and randomized rats was grouped into seven, orally treated with Indomethacin as the ulcerant group, groups of 62.5mg/kg, 125mg/kg, 250mg/kg and 500mg/kg extract as the experimental groups, Omeprazole as the positive control and no treatment group which serves as the control group. Upon oral and experimental processes, the rats were examined for a week and sacrificed on the 7 th day. Gastric ulcerations from the stomach of rats were observed by gross necropsy. Gross necropsy results showed significant difference in gastroprotective activity. The researchers found out that 500 mg/Kg concentration have gastroprotective effect which has a comparable result with the positive control, Omeprazole (20 mg/kg). The results from the negative control, Indomethacin 100 mg/kg showed that 2 of the test rats were found with severe ulcer/streaks as well as erosion in the gastric mucosa with 1 of the rats showing perforation. Omeprazole (20 mg/kg), on the other hand, managed to prevent the appearance of perforations, erosions and severe ulcer or streaks. This shows that as the concentrations get higher there is a higher gastroprotective effect and closer action to that of the positive control. TABLE OF CONTENTS Title Page i Approval Sheet .. ii Acknowledgement . iii Abstract ... iv Table of Contents .. v List of Tables .. vi List of Figures . vii CHAPTER I. INTRODUCTION ... 1 Background of the Study .. 1 Statement of the Problem .... Null Hypothesis .. 3 3 Significance of the Study .. 3 Scope and Delimitation . 4 Definition of Terms . 4 II. REVIEW OF RELATED LITERATURES AND STUDIES 6 The Plant Material .. 6 Folkloric Uses . 8 Phytochemical Characteristics . 9 Overview of Gastric Ulcer . 10 Treatment and Management of Gastric Ulcer Activity . 11 III. RESEARCH DESIGN AND METHODOLOGY . 15 Research Design 15 Research Methods . 15 Plant Collection and Extraction ..... 15 Test for Gastroprotective Effect..... 16 Gross Necropsy.... 18 Statistical Treatment.... 20 IV. PRESENTATION, ANALYSIS AND INTERPRETATION OF DATA . 21 The Plant Extract ... 21 Gastroprotective Screening of Flavonoids, Saponins and Tannins. 22 Solvent Testing ..... 23 GastroprotectiveEffect.. 24 V. SUMMARY, CONCLUSIONS AND RECOMMENDATIONS 28 Summary of Findings 28 Conclusions .... 29 Recommendations . 30 BIBLIOGRAPHY. 31 APPENDICES. 35 A. Certificate of Plant Authentication . 35 B. Documentation for Plant Preparation and Extraction.. 36 C. Documentation for Procurement and Physiological Adjustment of Rats .... 37 D. Experimental Procedure..... 39 E. Gross Necropsy... 40 F. Ulcerations of Stomachs.. 41 G. Computation of Doses. 42 H. Certification of Gross Necropsy . 46 I. Letter Seeking Permission...... 47 J. English Critic Certification 48
LIST OF TABLES Table Page 2.1 Phytochemical Screening of JasminumsambacLeaves 10 3.2 Ulcer Score and Descriptive Observation 20 4.1 Phytochemical Screening Results of Crude Leaf Extract 22 4.2 Gross Necropsy Result 24 4.3 Gastroprotective Property of Jasminumsambac 25
LIST OF FIGURES Figure Page 2.1 The Leaves and Flowers of Jasminum sambac 7 3.1 Flowchart of the Extraction Procedure 17 3.2 Experimental Procedure 19