Anaerobic

Microbiology

Section A

A
naerobic

icrobiology

A Culture of Service

Anaerobic Microbiology

A
A4 Tube Media A12 Anaerobe Jar

naerobic Microbiology

Table of Contents
A1 Introduction to AneroGRO A2 Plates - AneroGRO A5 Anaerobic Transport Media A6 AnaeroGen Gas Generators A8 CampyGen Gas Generators A10 CO2Gen Gas Generators A13 Identication Disks and Rapid Test Reagents

A Culture of Service
Headquarters 1430 West McCoy Lane Santa Maria, CA 93455 800.266.2222 : phone 805.346.2760 : fax Sales@HardyDiagnostics.com www.HardyDiagnostics.com Distribution Centers Santa Maria, California Centralia, Washington Salt Lake City, Utah Phoenix, Arizona Dallas, Texas Springboro, Ohio Lake City, Florida Albany, New York

The Quality Management System at the Hardy Diagnostics manufacturing facility is certied to ISO 13485. Copyright 2011 Hardy Diagnostics

Section A

aPre-reduced culture media packaged in oxygen-free, gas ushed foil pouches aPackaging contains an oxygen scavenger sachet, and a moisture absorbing
desiccant packet

aFoil pouches have an easy-to-open, pre-scored notch aWide variety of packaging combinations available: monoplates,
biplates, and primary set-up combinations Hungate caps

aTubes feature a screw-cap design with rubber septum aQuality Control tested for reliable and reproducible results aExceptional growth and performance characteristics
compared to other leading brands

aManufactured in an ISO 13485 certied facility aCompetitive pricing, quality products, with
exceptional service and support
The next time we need to place an order it will be Hardy. Our side by side comparison was unbelievable. The CLSs couldnt believe how much better the organisms grew on your media. With the cost savings, its a no brainer. Pat Wong Alta Bates Medical Center

A Culture of Service

A Culture of Service

Anaerobic Microbiology

A1

Easy-to-open Pre-scored Notch

Flushed with Oxygen-free Gas (inside bag)

Oxygen Scavenger Sachet (absorbs residual O2 after gas flushing)

3 plates/pack 15x100mm

Oxygen Impermeable Foil Pouch

Moisture Absorbing Desiccant

One Plate Per Foil Pouch

Description
Anaerobic PEA (Phenylethanol) Agar (monoplate) BBE (Bacteroides Bile Esculin) Agar/LKV (Laked Blood, Kanamycin, and Vancomycin) Agar (biplate) BBE (Bacteroides Bile Esculin) Agar (monoplate) Brucella Agar with Hemin & Vitamin K (monoplate) Campy Selective Agar (monoplate) CCFA (Cycloserine Cefoxitin Fructose Agar) (monoplate) Egg Yolk Agar, Modied (monoplate) LKV (Laked Blood, Kanamycin, and Vancomycin) Agar (monoplate) Brucella Agar with Hemin and Vitamin K (15x150mm monoplate)

Cat. no.
AG901 AG061 AG051 AG301 AG701 AG501 AG401 AG601 AG801

Pack Size
1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack 1 plate/pack

A2

Anaerobic Microbiology

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Two Plates Per Foil Pouch Description

DuoPak A Brucella Agar with H & K (monoplate), BBE Agar/LKV Agar (biplate)

Cat. no.

Pack Size

AG302

2 plates/pack

DuoPak B Brucella Agar with H & K (monoplate), BBE Agar/Anaerobic PEA Agar (biplate)

AG312

2 plates/pack

Three Plates Per Foil Pouch Description

MultiPak A Brucella Agar with H & K (monoplate), Anaerobic PEA Agar (monoplate), BBE Agar/LKV Agar (biplate) MultiPak B Brucella Agar with H & K (monoplate), Anaerobic PEA Agar (monoplate), LKV Agar (monoplate)

Cat. no.

Pack Size

AG303

3 plates/pack

AG313

3 plates/pack

Four Plates Per Foil Pouch Description

Brucella Agar with H & K Brucella Agar with H & K (monoplate)

Cat. no.

Pack Size

AG304

4 plates/pack

BBE/LKV Biplate BBE Agar/LKV Agar (biplate)

AG064

4 plates/pack

A Culture of Service

Anaerobic Microbiology

A3

Tube Media

Needleport Septum Hungate Cap

Anaerobic environment inside the tube

Description
Filtered to Remove Dead Bacteria Thioglycollate Broth with H & K, without Indicator, Hungate cap, 16x125mm tube, 9ml Thioglycollate Broth with H & K, without Indicator, Hungate cap, 16x125mm tube, 7ml Non-Filtered Media Chopped Meat Glucose Broth, Hungate cap, 16x125mm tube, 9ml Chopped Meat Carbohydrate Broth, Hungate cap, 16x125mm tube, 9ml Chopped Meat Broth, Hungate cap, 16x125mm tube, 9ml PY Glucose Broth Hungate cap, 16x125mm tube, 9ml
Anaerobic Microbiology

Cat. no.

Pack Size

AG22H AG23H

20 tubes/pack 20 tubes/pack

AG19H

20 tubes/pack 20 tubes/pack 20 tubes/pack 20 tubes/pack

AG20H AG21H AG24H

A4

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Anaerobic Transport Media

The Anaerobic Transport System is a sterile, ready-to-use collection kit, consisting of two sterile swabs (optional). Available in a peel pouch (optional) and a glass tube containing a semi-solid, pre-reduced transport medium. The sterile peel pouch is suitable for the surgical suite. The medium has an indicator that will turn lavender if the anaerobic condition is compromised. Fluids can be injected with a syringe directly into the tube through a pierceable, rubber septum. Maintains viability of a wide variety of microorganisms for up to 48 hours during transportation, by StarPlex Scientic.

Needleport Septum, Hungate Cap

Patient Information

Anaerobic Transport Medium

Includes two collection swabs, scored for easy break-off into the transport tube.

Description
Anaerobic Transport Medium, sterile surgery pack, Hungate septum screw-cap, no swab, 15x103mm glass tube, 7ml ll Anaerobic Transport Medium, sterile surgery pack, Hungate septum screw-cap, two swabs, 15x103mm glass tube, 7ml ll Anaerobic Transport Tube, non-sterile pouch, Hungate septum screw-cap, no swab, 15x103mm glass tube, 7ml ll
A Culture of Service

Cat. no.
S1200 S120D AG25H

Pack Size
100 tubes/case 100 tubes/case 20 tubes/pack
Anaerobic Microbiology A5

AnaeroGen
AnaeroGen Compact
AnaeroGen Compact is a simple-to-use system for rapidly generating an anaerobic environment necessary to cultivate anaerobic microorganisms. The AnaeroGen Compact system consists of an AnaeroGen sachet, oxygen impermeable pouch, and sealing clip. Each pouch is large enough to hold four 15x100mm petri plates.

No catalyst needed and no water required for activation No potentially explosive hydrogen gas produced No dangerous build-up of pressure, since sachet acts as an oxygen scavenger Sachet is activated upon opening Final atmosphere generated contains less than 1% oxygen, which is ideal for the growth of anaerobes Anaerobic conditions reached in 30 minutes

AnaeroGen Compact System

Sachets and Pouches 10 per box............................................................AN010C Sealing Clips 5/box (reusable)...................................................AN005C Anaerobic Indicator 100 strips/box..........................................................BR55

Sealing Clip

AnaeroGen Compact Sachet

O2 Impermeable Pouch

A6

Anaerobic Microbiology

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Jar System for Anaerobes

AnaeroGen is a simple-to-use system for rapidly generating an anaerobic environment necessary to cultivate anaerobic microorganisms within a sealed jar. The AnaeroGen system consists of an AnaeroGen sachet and an oxygen indicator within a gas jar. Each sachet generates enough gas for a 2.5 liter jar.

AnaBag 150
Hardy Diagnostics AnaBag 150 is a disposable environmental bag used for the incubation of 150mm plates in both anaerobic and CO2 enriched atmospheres. The AnaBag 150 is constructed from a triple laminated polymer, which forms a barrier to oxygen, CO2, and other gases. For use with Cat. nos. H05, H07 , H21, and AG801 products. (Not suitable for maintaining microaerophilic conditions.) Bags for 150mm plate, bag size 8.75x13.5, 20/pack..................................................................................AB20

AnaeroGen Jar System

AnaeroGen 2.5 liter Sachets 10 sachets/box.....................................................AN25US Almore Gas Jar, 2.5 liter Each.........................................................................16000 Anaerobic Indicator 100 strips/box..........................................................BR55

Almore Gas Jar

AnaeroGen Sachet

A Culture of Service

Anaerobic Microbiology

A7

CampyGen
CampyGen Compact
CampyGen Compact is a simple-to-use system for rapidly generating a microaerophilic environment necessary to cultivate Campylobacter spp. The CampyGen Compact system consists of a CampyGen sachet, an oxygen impermeable pouch, and a sealing clip. Each pouch is large enough to hold four 15x100mm petri plates.

No catalyst needed and no water required for activation No potentially explosive hydrogen gas produced No dangerous build-up of pressure, since the sachet acts as an oxygen scavenger Sachet is activated upon opening Final atmosphere generated contains 6% oxygen, which is ideal for the growth of Campylobacter and other microaerophilic organisms Microaerophilic conditions reached in 30 minutes

CampyGen Compact System

Sachets 20 sachets/box.....................................................CN020C Plastic Pouches 20 pouches/box...................................................AG020C Sealing Clips 5/box (reusable)...................................................AN005C

CampyGen Compact Sachet

CampyGen Compact Sachet

Sealing Clip O2 Impermeable Pouch

A8

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Jar System for CampyGen

CampyGen is a simple-to-use system for rapidly generating a microaerophilic environment necessary to cultivate Campylobacter spp. within a sealed jar. The CampyGen jar system consists of a CampyGen sachet and a gas jar. Each sachet generates enough gas for a 2.5 liter jar.

Campy Gas Tank

A 20 cubic feet gas tank, disposable, that contains microaerophilic gas (5% O2, 10% CO2, 85% N2) for the lling of gas impermeable bags for the culture of Campylobacter spp. Shipping requires a hazmat shipping charge. One tank will ll about 100 single plate bags, by Hardy Diagnostics. Campy gas bag, oxygen impermeable plastic, zip-lock: 6x6, 250/box.......................................................4742S 9x12, 250/box.....................................................4744S Campy gas tank, nozzle, brass, reusable, Each.........................................................................TN100 Campy gas tank, Each.........................................................................CG100 Mounting bracket, Each.........................................................................S0735

CampyGen Jar System

CampyGen 2.5 liter Sachets 10 sachets/box.....................................................CN025A Almore Gas Jar, 2.5 liter Each.........................................................................16000

CampyGen Sachet

Almore Gas Jar

A Culture of Service

Anaerobic Microbiology

A9

CO2Gen
CO2Gen Compact is a simple-to-use system for rapidly generating an increased CO2 environment ideal for growing capnophilic microorganisms such as Neisseria gonorrhoeae and Haemophilus inuenzae. The CO2Gen Compact system consists of a CO2Gen sachet, oxygen impermeable pouch, and sealing clip. Each pouch is large enough to hold four 15x100mm petri plates.

CO2Gen Compact

Sachet is activated upon opening Final atmosphere generated contains 6% carbon dioxide, which is ideal for the growth of capnophilic organisms Capnophilic conditions reached in 30 minutes

CO2Gen Compact

20 sachets/box....................................................CD020C Plastic Pouches 20 pouches/box..................................................AG020C Sealing Clips 5/box (reusable)..................................................AN005C CO2 Indicator (IndiKwik) 100 strips/box..........................................................G511

Sealing Clip

O2 Impermeable Pouch

CO2Gen Sachet A10 Anaerobic Microbiology

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Jar System for CO2Gen

CO2Gen is a simple-to-use system for rapidly generating an increased CO2 environment ideal for growing capnophilic microorganisms within a sealed jar. The CO2Gen system consists of a CO2Gen sachet and a gas jar. Each sachet generates enough gas for a 2.5 liter jar.

CO2Gen Jar System

CO2Gen 2.5 liter Sachets 10 sachets only......................................................CD025A Almore Gas Jar, 2.5 liter Each.........................................................................16000 CO2 Indicator (IndiKwik) 100 strips/box...........................................................G511

Almore Gas Jar

CO2 Gen Sachet

A Culture of Service

Anaerobic Microbiology A11

Anaerobe Jar
Classic! Proven! Reliable!
The Almore anaerobe jar is specially designed for temperature stability and durability. Its unbreakable, lightweight, transparent and, of course, autoclavable. Double screw clamp, coupled with the patented Quad-Ring gasket, ensures a more positive seal superior to conventional O-rings. Screw clamp lid also doubles as a handy carrying handle. The 3/8 inch thick anodized aluminum lid wont warp, and its guaranteed to resist cracking or breaking for ten years. Shipped complete with a stainless steel rack to accommodate 14 (15x100mm) standard sized petri plates. With stainless steel rack and catalyst basket...................................................................16000

Solid, durable, screw locking lid

Long-lasting anodized aluminum lid Quad-Ring gasketbest seal in the industry

Stainless steel rack for easy plate removal, holds 14 petri plates

Gas generating sachet

A12

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Single Disk Dispenser, Cat. no. 260457

Identication Disks and Reagents

Vancomycin, 5g, HardyDisks
For the differentiation of anaerobic bacteria based on susceptibility to vancomycin. Each cartridge contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50 disks/cartridge..................................................Z7501

Anaerobe Nitrate Reagent B

Cleves Acid for nitrate reduction test, by Hardy Diagnostics. 15ml, Each................................................................Z135

Nitrate Reagent C

Kanamycin, 1000g, HardyDisks

For the differentiation of anaerobic bacteria on the basis of susceptibility to kanamycin. Each cartridge contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50 disks/cartridge..................................................Z7191

Zinc dust for nitrate reduction test, by Hardy Diagnostics. 5gm.............................................................................Z73

Bile HardyDisks

Anaerobe Nitrate Reagent A

Sulfanilic acid for nitrate reduction test, by Hardy Diagnostics. 15ml, Each................................................................Z134

To aid in the identication of the Bacteroides fragilis group on the basis of bile resistance, by Hardy Diagnostics. 50 disks/cartridge..................................................Z7091

Bile HardyDisks, Cat. no. Z7091

Catalase Reagent, 15%

Anaerobe Nitrate Reagent A, Sulfanilic Acid for nitration reduction test, Z134. A Culture of Service

A 15% solution of hydrogen peroxide for the detection of catalase activity in anaerobic bacteria, by Hardy Diagnostics. 15ml dropper bottle ........................................................Z262
Anaerobic Microbiology A13

Colistin Sulfate, HardyDisks, Cat. no. Z8411

Carbon Dioxide (CO2) Tablets

Indole Nitrate Broth

Tablets individually sealed in ten-pack foil pouch. 1000/box..................................................................CO2TAB

Colistin Sulfate (CT), 10g, HardyDisks

For the differentiation of anaerobic bacteria based on susceptibility to colistin. 50 disks/cartridge........................................................Z8411

Indole Nitrate Broth

For the detection of nitrate reduction and indole production by microorganisms with a wide range of oxygen tolerances inculding aerobes, microaerophilic, facultative anaerobes, and obligate anaerobes. Contains a Durham tube for gas detection, by Hardy Diagnostics. 6ml, 16x100mm polycarbonate tube with Durham tube, 20/package..............................................................K147

Showing negative nitrate reduction reaction after the addition of Nitrate Reagent A (Cat. no. Z134) and Reagent B (Cat. no. Z135).Clostridium sordellii (ATCC 9714) grown in Indole Nitrate Medium (Cat. no. K147).

Showing positive nitrate reduction reaction after the addition of Nitrate Reagent A (Cat. no Z134) and Reagent B (Cat. no. Z135). Propionibacterium acnes (ATCC 29399) grown in Indole Nitrate Medium (Cat. no. K147).

A 1.5ml aliquot of the sample is transferred to a clean test tube. Showing a negative indole reaction after the addition of Indole Reagent (Cat. no. Z67). Clostridium perfringens (ATCC 13124) grown in Indole Nitrate Medium (Cat. no. K147).

A 1.5ml aliquot of the sample is transferred to a clean test tube. Showing a positive indolle reaction after the addition of Indole Reagent (Cat. no. Z67). Clostridium sordellii (ATCC 9714) grown in Indole Nitrate Medium (Cat. no. K147).

See also Rapid Tests & Reagents section, page P5.

A14

Anaerobic Microbiology

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Nitrocef MatchBook

Nitrate, HardyDisks

For the determination of nitrate reduction by anaerobes, by Hardy Diagnostics. 50 disks/cartridge.......................................................Z7071 The Nitrocef MatchBook is a rapid test (5 minutes) used to quickly pick colonies and perform the test at the same time! Easier and faster than a disk! The rapid Nitrocef test consists of a paper match-stick impregnated with the nitrocen reagent, which is a chromogenic cephalosporin. A positive -lactamase test can predict resistance to such antibiotics as penicillin, ampicillin, amoxicillin, and other -lactamase containing drugs for the following organisms: Neisseria gonorrhoeae Haemophilus spp. Moraxella (Branhamella) catarrhalis Staphylococcus spp. Enterococcus spp. Anaerobes (such as some species of Bacteroides, Porphyromonas, Clostridium, Fusobacterium, Prevotella)

Nitrocef Matchbook

PRO Disks are used for the rapid screening of Clostridium difcile from selective media. They may also be used as rapid spot tests for anaerobes and enterics, by Key Scientic Products. 50 disks....................................................................K1532

PRO Disks

SPS Disks

Chromogenic nitrocen test for beta lactamase, on paper strip, 10 tests/package......................................................Z108 Nitrocef Disks, 50/vial.....................................................................Z7301

For identication of Gardnerella vaginalis and Peptostreptococcus anaerobius. Each cartridge contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50disks/cartridge....................................................Z7381

Single Disk Dispenser

Compatible with all HardyDisks AST, by BD Bio-sciences. Each......................................................................260457

Colistin Sulfate, CT, HardyDisks, Cat. no. Z8411 A Culture of Service Anaerobic Microbiology A15

n a e r obic M icr obiolo gy

A16

Anaerobic Microbiology

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A Culture of Service

Anaerobic Microbiology

A17

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A Culture of Service

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