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Agricultural Biotechnology: ARS Main Exam-2012
Memory Based Paper
Developed by: IfAS, B-7 Saraswati Nagar, Jodhpur

Note: Time: 3 Hrs; MM – 240 (Part A: 40x2=80; Part B: 20x5=100; Part C: 6x10=60)
All questions are compulsory
Part A (Word limit- 10 words)
1. Nucleolous responsible for ……………………..
2. Which of the following is DNA binding domain
a. Helix-loop helix
b. Helix turn helix
c. Leucine zipper
d. Zn finger
Comments- All four/a and d/b and d/ c and a
3. Glucose and mannose are enantiomers (X/√)
4. In the ……………….. molecule move against concentration gradient
5. Arrange the following in increasing order according to insert size
6. Plasmid/ Phagmid/Cosmid/BAC/YAC
7. Glucose-6-phosphate is marker tag for lysosome targeting protein (X/√)
8. Cyclic and non cyclic photosynthetic reaction in the photosynthesis occurs to
regulate ……………. in the plant cell
9. Match the following
Southern blotting…..DNA
Northern blotting…….RNA
Western blotting…….Protein
10. Most condensed state of chromosome in mitosis is ………………..
11. Sexual reproduction in bacteria discovered by …………..
12. First transgenic tomato crop with the trait of increasing shelf life and delayed
ripening was developed using ……………………………
13. ATP and ADP have maximum charge at pH …………
14. ………. used as background for FTIR analysis
15. Define Exon………………………………………………………….
16. Protein acylation occurs during ……………………….
17. Why enzyme isolation carried out at 4ºC temperature…………………
18. Golden rice enriched with……………..
19. Ribozyme is…………………………..
20. All enzymes are protein in nature (X/√)
21. What is the role of osmolyte……….
22. What is pseudogenes………………………
23. GC box position in eukaryotic gene is at -70 (X/√)
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24. What is the jumping gene……………………..
25. Arrange the order according to decreasing sensitivity to ά aminitin (RNAP
I/II/III)…..
26. What is the role of oxidoreductase enzyme in the cell……………
27. TBP binding site present in all eukaryotic genes (X/√)
28. Tth act as reverse transcriptase for………..and DNA polymerase for……………
29. Match the following
RAPD………Dominant
AFLP……….Bi-allelic
RFLP……….Restriction enzyme
SNP………....Single primer use
30. What is SSR………………………….
31. A DNA fragment digested with two restriction enzyme and resultant get longer
DNA fragment, may be due to lack of……………….and …………………..
32. Somaclonal variation is due to differential gene expression (X/√)
33. Microarray is the technique to estimate gene expression level in which short
stretch of known DNA fragment is immobilized (X/√)
34. What is the central dogma……………..
35. What is the role of chaperon…………………..
36. Write the name of cis and trans element of eukaryotic gene………………………
37. What is the role of aminopterin in HAT medium used for the selection of
hybridoma cells
38. What is isomerase enzyme……………..
39. What is reporter gene………………………
40. What is TILLING…………………………….
Part B: (Word limit- one or two paragraph or graphical representation)
41. Difference in prokaryotic and eukaryotic cell
42. What is mitochondrial male sterility?
43. What is isoenzymes, give one suitable example
44. An organism having genome size of 4.2×10
6
.How much number of nucleosome
and each of octamer are required?
45. Explain covalent catalysis of enzyme activity?
46. What is the next generation sequencing? State principal and its three important
steps?
47. Explain enzyme inhibition and their effect on Km and Vmax?
48. What is bio-fortification?
49. Explain DNA double helix model?
50. Write the name of transcription regulating DNA binding domain and what type of
interaction between DNA and protein
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51. Conclude the statement: A single base change in the DNA stretch leads to change
the amino acid sequence or code truncated protein from the down stream of
changed nucleotide.
52. What is the enhancer element?
53. What is the role of trimeric G protein?
54. Write important steps of cell cycle regulation?
55. What is the role of HSp 70 protein?
56. What kind of morphological and physiological changes observed in drought
tolerance plant?
57. Write important steps of southern hybridization?
58. How genetic engineering helps in increasing shelf life and delay in ripening
process of tomato?
59. Write three essential features and four limitation of YAC?
60. How haploid production is possible through wide hybridization?
Part C (Word limit- Two pages)
61. Explain T DNA transfer mechanism
With the example of BT Cotton explain how genetic engineering play important
role in genetic engineering?
62. what is double haploid
Application of double haploid in the preparation of high density genome map
preparation
Important steps in genome map preparation
63. What is the secondary metabolite, write the name of three metabolites developed
from plat tissue culture, and explain why these metabolite is not harmful for cells
which are producing them?
64. What is the difference between chromosome walking and chromosome jumping
and how chromosome walking help in gene identification using high density
genome map?
65. What is cDNA library and how it differs from other DNA libraries?
How cDNA library helps in development of molecular marker?
66. Difference between structural and functional genomics?
What is the use of whole genome sequencing?
Explain ordered clone by clone approach?

NOTE: This question paper is absolutely based on memory, original questions and
contents may vary, and not applicable for any type of legal claims, for any error IfAS-
Jodhpur would not be responsible.


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