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CELL THEORY
What level of complexity is necessary for life?
• Aristotle (384 – 322BC)
SUMMARY
More is different!
• As a multicellular organism grows and develops it follows a structured plan
• The cells specialise (differentiate)
• The whole organism shows homeostatic control
• A developing multicellular organism shows emergent properties
• It is not just a the sum of the parts
CELL TYPES:
A. PROKARYOTIC CELLS
B. EUKARYOTIC CELLS
Prokaryotic Cells
“The cells in which true or complete nucleus is not present, instead, genetic material is
found scattered in the cytoplasm”
– pro = before, karyon = kernal (what nuclei looked like to those who first saw them
through a microscope)
– cells without organized nuclei bounded by a nuclear membrane
– Eubacteria (true bacteria), and cyanobacteria (blue-green algae)
– cell wall
– no membrane bound organelles
– nucleoid (main genetic content of the cell)
– Plasmids (small circular pieces of DNA that are separate from the DNA of nucleoid
Examples of rod-shaped bacteria. Top: Rod-Shaped Bacterium, hemorrhagic E. coli, strain 0157:H7
(division)
Spherical (cocoid) and spiral bacteria. Top: Coccoid-shaped Bacterium (causes skin infections),
Enterococcus faecium (SEM x33,370). Bottom: Left, a cross-section of a cell illustrating the location
of a flagella inside the cell; Center, Borrelia burgdorferi, the organism that causes Lyme disease;
and Right, Treponema pallidum, the spirochete that causes the venereal disease syphilis.
Eukaryotic Cells
“The cells having complete nucleus bounded with a nuclear membrane”
– eu = true, karyon = kernel (nucleus)
– cells with organized nuclei bounded by a nuclear membrane
– Examples: Plant cells, Animal cells, fungi, and protists (single celled organisms)
– cell wall in some
– plasma membrane in all
– Membrane bound nucleus
– membrane bound organelles
• "Endoplasmic Reticulum" (ER)
• "Ribosomes"
• "Golgi Apparatus and Dictyosomes" or Golgi look alike
• "Lysosomes"
• "Mitochondria"
• Chloroplasts ("Plastids“)
– Examples: red and white blood cells
Comparison of Prokaryotes and Eukaryotes
Prokaryotes Eukaryotes
Organisms Monera: Eubacteria and Protists, Fungi, Plants and Animals
Archebacteria
Level of single celled single celled (protists mostly) or
organization multicellular usually with tissues and organs
Typical cell size small (1 -10 microns) large (10 - 100 microns)
Cell wall almost all have cell walls fungi and plants (cellulose and chitin); none
(murein) in animals
Organelles usually none many different ones with specialized
functions
Metabolism anaerobic and aerobic; mostly aerobic
diverse
Genetic material single circular double complex chromosomes usually in pairs;
stranded DNA each with a single double stranded DNA
molecule and associated proteins contained
in a nucleus
Mode of division binary fission mostly; budding mitosis and meiosis using a spindle;
followed by cytokinesis
CELL STRUCTURE AND FUNCTION
DIFFUSION
• The principal means of passive transport
• It is the random movement of molecules from a area of higher concentration to an area of
lower concentration.
• the direction is determined by the concentration of specific molecules in the two sides of the
membrane and the energy that causes the diffusion
TYPES OF DIFFUSION
1. SOLID OVER
a. solid - MASTICATION
b. liquid - DIGESTION
c. gas - TRANSPIRATION
2. LIQUID OVER
a. solid - PERSPIRATION
b. liquid -DIGESTION
c. gas - EVAPORATION
3. GAS OVER
a. solid - SUBLIMATION
b. liquid - OXYGENATION
c. gas - RESPIRATION
FACILITATED DIFFUSION
- a solute binds to a specific
transporter
on one side of the membrane and is
released on the other side after the
transporter undergoes a conforma-
tional change.
Property of Diffusion
• The greater the space between these molecules the greater the ability for the molecular
particles to spread out from one another.
• The more packed the molecules are in the substance the less space to maneuver, and
therefore, the more difficult for diffusion to occur.
• Requires diffusion pressure
OSMOSIS
• is the movement of water molecules from a region of their higher concentration to a
region of their lower concentration, through a partially permeable membrane
• Water will move by osmosis into and out of cells due to differences in water potential
between the cell and its surroundings.
Water potential is the chemical potential of water and is a measure of the energy
available for reaction or movement (Bidwell 1974:59).
.
Water potential is a measure of the tendency of water to move from high free energy to
lower free energy.
• Distilled water in an open beaker has a water potential of 0(zero).
- The addition of solute decreases water potential.
- The addition of pressure increases water potential.
• In cells, water moves by osmosis to areas where water potential is lower.
– A hypertonic solution has lower water potential.
• A hypotonic solution has higher water potential
TONICITY
Hypertonic Solutions: contain a high
concentration of solute relative to another
solution (e.g. the cell's cytoplasm). When a cell is
placed in a hypertonic solution, the water diffuses
out of the cell, causing the cell to shrivel.
ACTIVE TRANSPORT
a. Primary Active Transport
- energy derived from hydrolysis of ATP changes the shape of a transporter protein
b. Secondary Active Transport
- energy stored in a Na ion or H ion concentration
gradient is used to drive other substances across the
membrane
against their own conc. gradients.
1. Antiporters – carry 2 substances
in opposite directions.
2. Symporters – carry 2 substances
in the same direction.
VESICULAR TRANSPORT
- formation of a vesicle or sac
1. Endocytosis
- materials move into the cell in a vesicle formed from the plasma membrane
a. Receptor-Mediated
- ligands
b. Phagocytosis
- solid particles
c. Pinocytosis
- tiny droplets of ECF
2. Exocytosis
- movement of materials out of a cell in vesicles that fuse with the plasma membrane.
a. Neurotransmitters
- neurons
b. Hormones and Digestive Enzymes
- secretory cells
The difference between phagocytosis and pinocytosis has to do with the size of the material
ingested.
1. Phagocytosis (cell eating) is shown above and occurs when solid material is involved. A white
blood cell phagocytosis bacteria when it ingests them and breaks them down inside the cell.
2. Pinocytosis (cell drinking) occurs when smaller particles, such as large molecules, that are in
solution are ingested by a cell. The process is the same as that shown above, but the type of
material taken into the cell differs
CELLULAR METABOLISM
• a major biochemical pathway along which the cells release the chemical bond energy from
the food and convert it to usable form (ATP)
• the many synthesis or breakdown of material taking place within the cell.
CELLULAR RESPIRATION
• the series of complex oxidation reactions whereby living
• cells obtain energy through breakdown of organic substances and other intermediate
materials.
• release of energy by the oxidation of fuel molecules by taking oxygen and release carbon
dioxide