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Symptoms of the susceptible variety will be rapid and include severe wilting and plant mortality. Response in the susceptible seedlings will be dramatic and clearcut. Record results in QC database and other relevant files.
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APPENDIX 9 - HT Spray Test Procedure for TT Canola
Symptoms of the susceptible variety will be rapid and include severe wilting and plant mortality. Response in the susceptible seedlings will be dramatic and clearcut. Record results in QC database and other relevant files.
Symptoms of the susceptible variety will be rapid and include severe wilting and plant mortality. Response in the susceptible seedlings will be dramatic and clearcut. Record results in QC database and other relevant files.
Procedure for Spray Test of Triazine Tolerant Canola
Updated Dec 2013
MATERIALS
Equipment: 2L Sprayer (calibrated)
Chemical: Gesprim (or similar chemical) Active constituent: 600g/L Atrazine
Potting Mix: Available from research bunker
Seed: Representative sample of line being tested Susceptible line (ie. susceptible control) Tolerant line that has been tested and passed (ie. tolerant control)
Trays: 2 x 100 cell grow trays (for variety to be tested) 1 x 100 cell grow tray ( for susceptible control, for tested & passed line)
Herbicide rate: Mix 5ml chemical/250ml water
Sprayer: Pump to maximum pressure
METHOD
Using potting mix, fill all trays. Sow a number of seeds from each line into each cell of relevant trays, water lightly and grow using normal horticulture practices. Label trays with: Variety, batch, grower, date planted, name of test (TT).
When seedlings appear, thin cells, allowing for attrition i.e. keep a number of extra plants to achieve correct population. Before spraying remove extra plants, leaving 100 plants per tray.
Plants should be sprayed at two to four leaf stage. Align trays to be sprayed lengthwise in suitable area for spraying. Apply herbicide to dry foliage holding spraying wand at height to produce a band width of 32cm. Spray trays walking at medium pace with one sweep. Label trays with date sprayed and name of test (TT).
ASSESSMENT
Triazine Response takes approximately 7 days or less. Symptoms of the susceptible variety will be rapid and include severe wilting and plant mortality. Response in the susceptible seedlings will be dramatic and clearcut.
Record results in QC database and other relevant files.
If results obtained from the spray test differ from what is expected, note it in the QC database and refer to the Genetic Purity Manager immediately to discuss further action.