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UNCOOKBOOKING A SCIENCE LAB

Evaluation of Laboratory Lesson ___The Need for Speed- A Look at Enzyme At!"!ty_(before and after)__________________________
I# the $a% an e&per!menta$ $a% 'to te#t a hypothe#!#() an *o%#er"at!on* $a% 'to #ee and o%#er"e m!roor+an!#m#) $earn ho, to -#e a
m!ro#ope) o%#er"e and ompare m!nera$#) $ea"e#) m!&t-re#) et.() or an *en+a+e* $a% at!"!ty 'to #et a #enar!o for the
#!ent!f! /-e#t!on) to %-!$d !ntere#t and !ntr!+-e !n a top!) to !dent!fy m!#onept!on#(0 Ident!fy !t a# #-h) #o #t-dent# don1t
th!nk e"ery *$a%* !# an e&per!ment.* A$$ $a%# and mo#t $e##on# an !n$-de 2eat-re# of S!ent!f! In/-!ry3
Features
o
f
I
n
q
u
i
r
y
Element present ? Level ? Explanation for assignment of Level
A Yes !ea"#er provides all questions
After Yes $ !ea"#er provides most questions until t#e end %#en t#e student must "onstru"t t#eir
o%n qui& using t#e information on listed %eb sites
' Yes $ (tudent follo%s dire"tions for every part of t#e lab and "olle"ts spe"ifi" data
Yes ) (tudents must "onstru"t t#eir o%n %ays of testing denaturation and "ompetitive
in#ibition*
+ Yes (tudents are required to present t#eir findings to t#e rest of t#e "lass, #o%ever t#ere is
an element of explanation missing* !#e students must simply loo- over t#e
grap# t#ey made and present t#eir data as a group*
Yes ) (tudents no% must present t#eir ideas and "ome up %it# an argument as to %#y t#eir
model for denaturation and "ompetitive in#ibition %as better t#an anot#er
groups* !#ey must also explain t#e trends t#ey #ave found and %#y t#ey
t#oug#t t#is %as o""urring*
. Yes / !#ere are no outside sour"es used to furt#er t#e students understanding or inquiry*
Yes $ !#e students are instru"ted to go to t#e provided %ebsites and "onstru"t a qui&* !#is
%ill furt#er t#eir understanding of t#e topi"s "overed* In t#e possible
assessments, t#e students are also required to resear"# 0 en&ymes using %#at
ever means ne"essary*
E 1o 0 (tudents must present t#eir grap#, #o%ever students are not required to "onstru"t t#eir
o%n arguments or t#in- outside t#e box in any %ay*
Yes / (tudents are required to "ompare t#eir finding %it# t#at of ot#er students and ma-e a
point of argument as to %#i"# %ay of testing demonstrated t#e spe"ifi"
situations*
The Need for Speed 4
A Look at Enzyme At!"!ty
The Need for Speed5A Look at Enzyme At!"!ty
Introd-t!on6
As s"ien"e tea"#ers, %e reali&e #o% important en&ymes are to living organisms* I #ave found it diffi"ult to "onvey t#is importan"e to students
be"ause it is a sub"ellular pro"ess t#ey "annot see and expensive to dupli"ate in a lab* !#is a"tivity provides a #ands2on and inexpensive met#od
for students to learn t#e fun"tion of en&ymes*
7-rpo#e6
!#e purpose of t#is lab is to give students an opportunity to demonstrate en&yme rea"tions and t#e environmental variables t#at affe"t t#ese
rea"tions*
O%8et!"e#6
(tudents %ill be able to3
4understand t#e rea"tion rates of an en&yme2mediated rea"tion*
4understand t#e effe"ts of environmental variables on en&yme fun"tion*
5elate t#eir findings to real %orld situations
Argue %#i"# test %as most effe"tive in demonstrating en&yme properties
4"olle"t, grap#, and analy&e data relating to t#e en&yme rea"tion*
9ater!a$#6
(tudents %ill %or- in teams of t#ree* Ea"# team of t#ree %ill need3
460 pennies
40 ni"-els
4roll of mas-ing tape
4tennis ball
4t%o2feet of rope
4glove
4 five buttons (various si&es)
4s"issors
4t%o index "ards
40 popsi"le sti"-s
4s#oe box or lab tub
40 marbles
7reparat!on6
In order for students to ans%er t#e questions in 78uestions to As-,9 t#ey s#ould #ave read about proteins and en&ymes or #ave previously
learned it in t#e "lassroom* !#is lab may be used to reinfor"e a lesson on proteins and en&ymes, a lesson on protein synt#esis,
en&ymes in t#e digestion system, or per#aps %#en tal-ing about addi"tion and en&ymes t#at destroy neurotransmitters* (tudents need to be
familiar %it# -ey %ords su"# as3 noncompetitive inhibition, denaturation, and competitive inhibitors* Allo% approximately )6 minutes for t#e
students to "omplete t#e lab and approximately $0 minutes for students to present t#eir pro"edures and grap#s*
7roed-re6
* !#e tea"#er %ill distribute 600 pennies on t#e floor*
/* :ne team member %ill attempt to pi"- up as many pennies as possible in 0 se"onds* !#e team member pi"-ing up t#e pennies must observe
t#e follo%ing rules3
a* ;i"- up one penny at a time
b* !a-e it ba"- to t#e group table
"* Lay it do%n FA+E <;*
$* !#e ot#er team members %ill be responsible for "ounting and "orre"tly re"ording t#e number pi"-ed up* =rite t#e data in t#e !eam .ata
!able under !rial *
)* !#is pro"ess %ill be repeated for six 02se"ond intervals*
6* .o not return t#e pennies until t#e end of !rial *
>* <sing t#e materials given, ea"# group %ill demonstrate bot# denaturation and "ompetitive in#ibition* (tudent groups s#ould design t#eir o%n
models for denaturation and "ompetitive in#ibition and obtain t#e tea"#er?s approval before pro"eeding to data "olle"tion* * ( E@AA;LE3
(tudents may tape t#eir fingers toget#er or use t#e glove as an example of denaturation* (tudents may tape t#e ni"-els, marbles, or t#e tennis
ball to t#eir #and to represent "ompetitive in#ibition*)
Li-e !rial , t#e students are to #ave six to 0 se"ond intervals, and re"ord t#e numbers on t#e !eam .ata !able*
B* <sing but"#er paper, students %ill %rite up t#eir labs22materials used, pro"edure, et"*22and ma-e a line grap# for ea"# set of data on t#eir
!eam .ata !able*
C* Instru"t t#e students to "ompare t#eir denaturation and in#ibitor tests to anot#er group* =#i"# model demonstrated t#e effe"ts of
denaturation better %#i"# one demonstrated "ompetitive in#ibition better? (upport your argument using t#e grap#s you "onstru"ted*
Safety6
In t#is lab, t#ere are no #a&ardous materials ot#er t#an t#e s"issors* If t#e marbles fall on t#e floor, t#ey pose a ris- of an a""ident* It is
ne"essary for students to stay %it# t#eir groups at all times*
:-e#t!on# to A#k
=#at is t#e importan"e of en&ymes for living beings?
* =#at is a protein?
/* Do% are proteins made?
$* =#y does .1A #ave to send a 7messenger9 to ma-e proteins?
)* =#at is a "atalyst?
6* =#y do %e need en&ymes?
>* =#at is an a"tive site?
B* =#at is a substrate?
C* =#at is an in#ibitor?
;eferene# and ;e#o-re#6
* +ampbell, Aary* (EE) Biochemistry* +#i"ago, IL3 (aunders +ollege ;ublis#ing*
/* Essenfield, 'erni"e, Fontang, +arol, and Aoore, 5andy* (EE>)* Biology* Aenlo
;ar-, +A3 Addison2=esley ;ublis#ing +ompany*
$* =essells, 1orman and Dopson, Ganet* (ECC)* Biology* 1e% Yor-3 5andom
Douse ;ublis#ing*
S-++e#t!on# 2or A##e##ment6
(tudents s#ould "omplete t#eir lab and present it to t#e "lass* !#e presentation s#ould in"lude t#eir #ypot#esis, experimental pro"edures, data
tables, grap#s, and follo%2up questions*
Dave t#e students? resear"# 0 en&ymesH list t#eir fun"tions and possible drugs or ot#er substan"es t#at in#ibit t#eir fun"tion and %#y t#ose
en&ymes must be in#ibited*
The Need for Speed -- A Look at Enzyme At!"!ty
St-dent At!"!ty Sheet
Team <ata Ta%$e
Time (in seconds)
Trial 1
Normal Enzyme
Activity
Trial 2
Denaturation
Trial 3
Competitive
Inhiition
=->= #eond#
>>-?= #eond#
?>-@= #eond#
@>-A= #eond#
A>-B= #eond#
B>-C= #eond#
Tota$
Ans%er t#ese questions on an atta"#ed s#eet of paper*
* In t#is a"tivity, %#at obIe"t represented t#e en&yme? t#e substrate? t#e in#ibitor?
/* In !rial , %#y did t#e rate eventually de"rease? =#at "ould #ave been added to
maintain t#e initial rate? .es"ribe t#e trend in trial * .id it go up, do%n, stay t#e same? =#y do you t#in- t#is o""urred?
$* If more substrate %ere present in !rial at t#e beginning, %ould t#e initial rate #ave been #ig#er? =#y or %#y not?
)* If %e assume t#at t#e en&yme is represented by t#e #and, %#at #appened to t#e a"tive site during !rial /?
6* =#y does an en&yme not %or- as %ell if its a"tive site is "#anged?
>* =#at environmental fa"tors affe"t t#e en&yme s#ape?
B* =#at effe"t did in#ibition #ave upon t#e rea"tion rate?
C* Do% mig#t "#emi"als affe"t you if t#ey a"ted li-e in#ibitors during your bodily rea"tions?

Follo%2up
Fo to t#e follo%ing %ebsites J "onstru"t a 0 point qui& t#at may be administered to ot#er groups*
http:// www.cel lsalive.com/cgi- bin/search/search.pl?Terms=enzyme
http:// www.chem4ki ds.com/fi les/bio_enzymes.ht ml