BIOL 371 GENETICS, CELLULAR AND MOLECULAR BIOLOGY I

Fall, 2014

INSTRUCTOR: Dr. M. Murray TELEPHONE: 471-6243
E.MAIL: mmurray@andrews.edu
OFFICE: PH 225
LECTURE: MWF:10:30-11:20AM PH106
OFFICE HOURS: W: 1:00-2:00 and R:12:30-2:00
LABORATORY: R.2:30-5:15pm or 6:30-9:15 pm
PH240
TEXT: Hartwell, L. 2008. Genetics: From Genes to Genomes 4
th
Ed. McGraw-Hill.
ISBN: 978-0-07-352526-6
PREREQUISITES: BIOL 166 and completion of or simultaneous enrollment in CHEM131.

COURSE DESCRIPTION:
The course is designed to introduce students to the basic principles of transmission, molecular and population
genetics. Emphasis is placed on Mendelian and Non-Mendelian inheritance, the nature of gene structure,
function and regulation, and modern discoveries of molecular biology and their applications in today's world.

LEARNING OUTCOMES:
Upon completion of this course students will be able to integrate concepts learned to solve genetics problems,
apply knowledge gained to real-world situations and apply the scientific method in a research project.

HOW TO DO WELL IN THIS COURSE:
Learning genetics is very challenging and time consuming. It requires an approach similar to that needed for
math or chemistry. Analytical thinking is the most important skill necessary for understanding
genetics. Memorization skills will help you, but not as much as in some other disciplines of biology.

A good understanding of genetics requires integration of mathematical analysis with a firm understanding of
biological events. The best way to achieve this is to work lots of problems. There are lots of problems at the
end of each chapter. READ the chapters and make sure you understand the figures and diagrams. Any
student who is too busy to come to class, study, and take exams; please consider withdrawing today.

Listening and paying attention in class, writing down only occasional notes will do you more good than trying
to write down all the words on a slide. Having written text is MUCH less helpful than trying to understand
methods and concepts. Genetics is about living things, therefore keeping in mind organisms, cells, and
chromosomes, will help the concepts make much more sense.



COURSE INFORMATION AND REQUIREMENTS

LECTURES: Will emphasize the main concepts of the course. Lecture outlines will be posted online and
may be used as a guide during class. Clicker and discussion questions will be administered during lecture at
the instructors discretion.

CASE STUDIES: Will be used to introduce new material and/or reinforce material covered during lecture.

HOMEWORK: There will be 7 homework sets due the Monday following assignment by 5pm. Late
homework will not be accepted. Feel free to work with your classmates in solving these problems, but
complete each assignment yourself. Students who rely on other students to actually work the problems, often
do very badly on quizzes and exams.

EXAMS: Will be on material covered in class, homework, recommended end-of-chapter problems (listed on
the schedule in parentheses), case studies/group work and any related material in your text. There will be 3
exams given during the semester each worth 100 points. The final will consist of two exams each worth 100
points. One will be on the material covered after the third test; the other will be comprehensive. The lowest
of the five exam grades will be dropped. As in all serious testing environments, you cannot leave the room
during an exam to get a drink, go to the bathroom or for any other reason, and then expect to resume testing.
If you have a health problem that prevents you from remaining in the classroom until you have completed the
exam, inform me and I will arrange special test taking accommodations.

QUIZZES: Will be given in both lab and lecture. Lab quizzes will be given during the first five minutes of
lab and will cover the introductory portion of the lab. You will not be allowed to makeup the lab quiz if you
are late. Lecture quizzes will be given using the clicker classroom response system and will be on material
covered during the previous and/or current lecture and material from your text to verify that the chapters are
being read. Makeup quizzes and exams will only be given under the following circumstances. (1) Medical
reason with report from doctor or nurse, containing legible name of both patient and health care professional,
and a contact number for verification. (2) Death in the family, with funeral notice or obituary for
confirmation. (3) Attending official AU business (Orchestra or Choir trip etc.) with a written statement from
a teacher or dean.

LABS: Attendance is mandatory even if you are repeating the course. Lab outlines will be posted online.
They should be printed and brought to lab. Data sheets must be handed in at the end of the lab. Some labs can
be completed in one session, others require several sessions and working outside of scheduled lab times. In
addition to the 9 labs listed in the syllabus, a research project based on research questions listed on moodle
will be conducted. The project is worth 100 points (paper + presentation). Points earned from the presentation
will be based on teacher and peer evaluation.

CHEATING: Cheating and other forms of academic dishonesty as defined in the Andrews University
Bulletin will result in a failing grade. It is the student's duty to avoid any appearance of cheating.

STUDENT CONDUCT: The instructor will enforce and students are expected to follow the University's
Student Conduct Code. Disruptive classroom behavior that substantially or repeatedly interrupts either the
instructor's ability to teach, or student learning, is prohibited. Examples include ringing cell phones, text-
messaging, watching videos, playing computer games, doing email, or surfing the Internet on your computer
instead of note-taking or other instructor-sanctioned activities. Although you may think that you are not
affecting others, these activities are a distraction to your peers.

GRADING:
Component *Approx. Points
Homework *100
Lecture exams 300
Lecture final 200
Lab reports/data sets *150
Case stud. *70
Research Project Paper 50
Research Project Presentation 50
Lab/clicker quizzes *70
Approx. Total *940
A
-

-
B+= 7786%, C
-
C+= 6776%, D= 5066%, F = < 50%

Finally, we each have our own responsibilities and workloads. Mine is to prepare learning materials,
lecture, answer questions, prepare exams, grade exams, and calculate your achievement toward mastering
genetics. Yours is to learn the material, come to class, turn in assignments, study for exams, take exams
on time, and contribute positively to the learning environment.



SCHEDULE
September

October
Monday Wednesday Thursday Lab Friday
25 August

Introduction
27 Mendelian Genetics
Chp. 2 (10, 15, 16, 17,
25, 29, 32)
28 Intro. to D. Melanogaster
&
Start Monohybrid Cross
29 Mendelian Genetics
Chp. 2
Case study

1 Labor Day

3 Extensions to
Mendelian Genetics
Chp.3 (6,9,18,20, 22,
31, 33)
4 Extensions to Mendelian
Genetics Chp.3

Remove P
1
adults
5 Extensions to
Mendelian Genetics
Chp.3
8 Chromosome Theory
of Inheritance: Meiosis
Chp. 4 (4,9,12,20,25)
10 Chromosome
Theory of Inheritance:
Meiosis Chp. 4
11 Mitosis in onion root tips

Cross F
1
s
12 Case study

15 Linkage, Recomb.
and Mapping Chp. 5 (3,
9, 20)
17 Linkage, Recomb.
and Mapping Chp. 5
18 Remove F
1
adults

Research proposals due
19 Linkage, Recomb.
and Mapping Chp. 5
22 DNA Structure and
Function Chp. 6 (3, 10,
13, 16, 17)
24 DNA Structure and
Function Chp. 6
25 DNA isolat. and quant.

Start Counting F
2
s

26 Test 1:Chps. 2,3,4,5
29 Genetic Analysis via
Mutations: Chp. 7
Chp. 7 (2, 16, 21, 25, 26,
30, 31)
1 Genetic Analysis via
Mutations: Chp. 7

2 Complementation
Testing (I)

3 Gene Mutations
Chp. 7
6 Gene Expression
Chp. 8 (11, 19, 25, 26)

8 Gene Expression
Chp. 8

9 Complementation
Testing (II)
10 Case Study
13 Fall Recess 15 Eukaryotic
Chromosome Chp. 12

16 Chromosomal
Mutations Chp. 13
17 Analysis of
Genetic Information
Chp.9 (5, 6, 15, 25,
30)
20 Analysis of Genetic
Information
Chp.9

22 Analysis of Genetic
Information Chp. 10
&11
23 DNA fingerprinting
and Microarray
24 Test 2:
Chps. 6,7,8, 12, 13
27 Prokaryotic Genetics
Chp. 14 (3, 5, 14, 16)
29 Prokaryotic Genetics
Chp. 14
30 Plasmid Identification/
Characterization (Bacterial
Transformation)
31 Organelle
Genetics Chp. 14





November



December

3 Gene Regulation:
Prokaryotes Chp. 15 (11,
12, 13, 19)
5 Gene Regulation:
Prokaryotes
Chp. 15
6 Plasmid Identification/
Characterization (extraction
and digestion)
7 Case Study
10 Gene Regulation:
Eukaryotes Chp. 16
12 Gene Regulation:
Eukaryotes Chp. 16
13 Plasmid Identification/
Characterization (Gel
electrophoresis and analysis)
14 Gene Regulation:
Eukaryotes Chp. 16
17 Test 3:Chps. 9, 10,14 19 Population Genetics
Chp. 19
20 Research Projects
Presentations
(2:30-5:15 PM)
21 Population
Genetics Chp. 19
24 No class

26 Thanksgiving 27 Thanksgiving 28 Thanksgiving
1 No class 3 No class 4 Research Projects
Presentations (6:30-9:15 PM)
5 No class
8 No class 10 Lecture Final
(10am-12pm)
11 12