BCH 6206: METABOLIC CONTROL ANALYSIS

TENTATIVE SCHEDULE: FALL 2011

M, W, F - Period 2 (8:30 AM - 9:20 AM), Room R3-265

In this course, we will discuss metabolism at a more advanced level than in the introductory courses,
with a focus on how we know, what we know. Students are expected to have performed with a B or better in
an introductory course in Biochemistry. Courses such as BCH 4204, CHM 4207, CHM 3218, BCH 3025 or
GMS 6001 are considered appropriate introductory courses for BCH 6206.
The text, Biochemistry by Voet and Voet (3
rd
Edition), will serve as a general reference. This is
available in the Health Science Center Bookstore. Older versions are also acceptable, although the pages of
interest may be somewhat different from the 3
rd
edition. Outside reading in primary journals, reviews, and
special monographs will be assigned when appropriate. There will be a heavy reliance on class notes
throughout the course.
Each lecture title (see syllabus e-learning in Sakai) represents the general area to be covered. The first
five weeks or so are devoted to discussing the tools that we use to study metabolism. The second five weeks
or so are devoted to signaling as applied primarily to carbohydrate metabolism. The final five weeks are
devoted to selected topics in lipid/steroid synthesis, and amino acid, and nucleic acid metabolism.

EXAMINATION SCHEDULE

There will be three (3) - three hour examinations. All three will be composed of essay, short answer,
and other question types.
Room
Tuesday 9/27 6:00 - 9:00 PM (Lectures 8/22 - 9/21) (C1-4)
Tuesday 11/1 6:00 - 9:00 PM (Lectures 9/23 - 10/26) (C1-4)
Monday 12/13 9:00 - 12:00 AM (Lectures 10/28 - 12/6) (C1-4)

CORE INSTRUCTORS

Dr. Susan Frost, Professor (Course Coordinator) R3-216A sfrost@ufl.edu 392-3207

Dr. Daniel Purich, Professor (Biochemistry) R3-126 dlpurich@ufl.edu 392-6878

Dr. Arthur Edison, Professor (Biochemistry) R3-226B aedison@ufl.edu 392-4535

Dr. Nicholas Simpson, Research Associate Professor
(Medicine) P1-31 simpsn@ufl.edu 846-2723

Dr. Joanna Long, Associate Professor (Biochemistry) LG-187 jrlong@ufl.edu 846-1506

Dr. Brian Cain, Professor (Biochemistry) R3-254 bcain@ufl.edu 392-6473

Dr. Michelle Gumz, Assistant Professor (Medicine) CG-92B michelle.gumz@medicine.ufl.edu
273-6887


E-learning: http://lss.at.ufl.edu

Enter Sakai and provide gatorlink user name and password
2010 Tentative Lecture Topics for BCH 6206

Date Topic Instructor

WEEK 1 M 8/22 Introduction to metabolic control Frost

W 8/24 Tools of metabolism Frost

F 8/26 General Introduction to NMR Simpson

WEEK 2 M 8/29 Isotopomer analysis: part 1 Simpson

W 8/31 Isotopomer analysis: part 2 Simpson

F9/2 Introduction to Metabolomics Edison

WEEK 3 M 9/5 Labor Day (No Class)

W 9/7 Metabolomics: statistical analysis Edison
and data mining

F 9/9 Metabolomics: published examples Edison

WEEK 4 M 9/12 Enzyme cooperativity and allosteric Purich
transitions

W 9/14 Radioisotopic tracers: Purich
protein turnover and pathway kinetics

F 9/16 Metabolic control analysis Purich

WEEK 5 M 9/19 Using metabolic inhibitors to Purich
understand pathways

W 9/21 Dan..last year you omitted a lecture in this series for lack of
time.you can have it back this year if you feel like you could
expand something.as I am eliminating the in-class review????

9/23 would begin the material for second testing cycle

F 9/23 Membrane structure: lipids Frost

WEEK 6 M 9/26 Membrane structure: proteins Frost

Tu 9/27 *First Exam (6-9 PM) (normally Th, but Rosh Hashanah starts on W)
(includes 13 lectures of material)

W 9/28 Facilitated nutrient transport Frost

F 9/30 Active nutrient transport Frost

WEEK 7 M 10/3 Signaling: G-protein receptors Frost
Date Topic Instructor


W 10/5 Signaling: tyrosine kinase receptors Frost

F 10/7 Signaling: steroids and lipids Frost

WEEK 8 M 10/10 Lipid rafts as signaling platforms Frost

W 10/12 Role of lipid in macromolecular synthesis Frost
and targeting: part 1

F 10/14 Role of lipid in macromolecular synthesis Frost
and targeting: part 2

WEEK 9 M 10/17 Nutrients as signaling molecules: part 1 Frost

W 10/19 Nutrients as signaling molecules: part 2 Frost

F 10/21 Counter-regulation of glycolysis and Frost
gluconeogenesis

WEEK 10 M 10/24 Counter-regulation of glycogen turnover Frost

W 10/26 Circadian rythyms and central pathway Gumz
Regulation

10/28 would begin the material for the third testing cycle

F 10/28 Fatty acid oxidation Frost

WEEK 11 M 10/31 Regulation of the TCA cycle Frost

Tu 11/1 Second Exam (6-9 PM)
(includes 15 lectures of material)

W 11/2 Energy conservation (or not) Frost

F 11/4 Homecoming (No Class)

WEEK 12 M 11/7 F
1
F
O
ATP synthase Cain

W 11/9 Fatty acid biosynthesis Gumz

F 11/11 Veterans Day (No Class)

WEEK 13 M 11/14 Prostaglandin metabolism Gumz

W 11/16 Cholesterol metabolism Gumz

F 11/18 Lipoprotein metabolism Gumz

WEEK 14 M 11/21 Nitrogen homeostasis Purich

Date Topic Instructor

W 11/23 Amino acid metabolism: part 1 Purich

F 11/25 Thanksgiving Break (No Class)

WEEK 15 M 11/28 Amino acid metabolism: part 2 Purich

W 11/30 Nucleotide metabolism part 1: Purich
synthesis, turnover, and salvage

F 12/2 Nucleotide metabolism part 2: Purich
synthesis, turnover, and salvage

WEEK 16 M 12/5 Folate coenzyme synthesis: Purich
one-carbon metabolism

W 12/7 Heme biosynthesis Purich


WEEK 17 M 12/12 Third Exam (9AM-12PM)
(includes 15 lectures of material)