APARAJITA SHANKER

Greater New York City Area | (C) (718) 915 2547 | Aparajita.Shanker@yahoo.com |www.linkedin.com/in/aparajitashanker

Summary
Biotechnology graduate with research experience in molecular biology and protein purification.
Skills
Microsoft Office, C, Ubuntu

Work History

Intern, R&D Jan 2014- Apr 2014
TechnoVax , Inc. New York
Developed broadly neutralizing vaccine against flu virus.
DNA transformation and cloning to develop virus like particles VLPs, Transfection of the VLPs into
mammalian cell lines and analysis of proteins using dot blot and western blot.
Developed protocols for protein purification with affinity chromatography using BIOCAD 700E.

Research Internship/Individual Projects, Aug 2012- May 2013
St Johns University New York
Introducing mutations into genes of interest to Parkinsons disease and transformation into cloning
and expression vectors.
Genome Annotation - Genome annotation of D.biarmipes.
Regulation of recombinant Erythropoiesis stimulating agents - Critical analysis on the history of
regulation of EPOGEN, an ESA manufactured and distributed by Amgen Pvt Ltd.,
Comparison of old vs. new biotechnology using the example of development of plant based vaccine
against Y. pestis - A comparison of several different vaccines generated and the possibilities of
development of improved and effective vaccine production using plants as medium.
Research Assistant Analytical Development Oct 2011- Aug 2012
Semler Research Center India
Daily activities included research work on multiple projects related to pharmaceutical development of
dry and liquid formulation of active ingredients.

Developed protocols for assay and related substances on HPLC, dissolution, Differential Scanning
Calorimeter and Thermo Gravimetric Analysis for new formulation drugs
Validated protocols for assay and related substances on HPLC, dissolution, KF Titrator, FTIR and UV-
Vis spectroscopy for new formulation drugs.
Performed API(Active Pharmaceutical Ingredient) characterization in accordance with USP, BP and
PH. Eur.,
Performed stability studies for new formulation drugs and marketed drugs.
Performed permeability studies using Franz Diffusion cell.
Trained by Waters Company on Method development in HPLC and use of new Empower software
Trained on Ultra Performance Liquid Chromatography conducted by Waters Company.
Independent Project Sept 2010- June2011
Dayananda Sagar College of Engineering India

Preliminary testing of antimicrobial activity of coconut-shell liquid extract - Lead a team to establish
the antimicrobial activity of the coconut-shell extract. The shell-extract was analyzed to exhibit in-
vitro antimicrobial activity against several multidrug strains which are clinically competent. The
sample isolated from the coconut shell was extracted by three methods.
Performed various biochemical, immunology and molecular biology assays.

Internship, Quality Control Department Oct 2009
Biocon Pvt. Ltd India

Operated HPLC, FTIR, UV Spectroscopy, Gas Chromatography.
EDUCATION

M.S in Biological & Pharmaceutical Biotechnology Aug 2012- May 2014
St Johns University 3.7 GPA New York

Bachelors of Engineering in Biotechnology Sept 2007- June 2011
Dayananda Sagar Institutions 73% India

Technical Skills

Cell culture and transfection into mammalian cell lines.
Molecular cloning, PCR
DNA extraction and purification(mini, maxi and mega prep)
Gel electrophoresis ( agarose and SDS-PAGE)
Protein Purification using HPLC and affinity chromatography(BIOCAD 700E and peristaltic pump)
western blot and dot blot analysis
Data Analysis, scientific protocol writing.
Analytical problem solving
General lab duties