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Application Note
0062 - Schisandra for Schisandrins by HPLC
As published in The Handbook of Analytical Methods for Dietary Supplements
Botanical Name: Schisandra chinensis
Common Names: Wu-Wei-Zi
Parts of Plant Used: Berries
Uses: Liver protection, adaptogen
Modes of Action:
A berry extract standardized with total lignans was pharmacologically
and clinically proven to be a good liver protective herb. In China, a clinical
trial with more than 5000 patients with different types of hepatitis was
performed in the 1980s. The patients were treated with a schisandra fruits
preparation. The elevated GPT (glutamic pyruvic transaminase, an enzyme
found primarily in the liver, which is released into the bloodstream as the result
of liver damage) level returned to normal with 75% of patients after they were
treated for 20 days with schisandra.
1
Schisandra also was found to improve physical
performance and was used to prevent and to treat neurodegenerative diseases.
2
Chemical Markers:
Shisandra chinensis is a rich source of lignans. More than 30 lignans have been puried from it with schisandrol A
(other names: schisandrin, wuweizi alcohol A, wuweizichun A), schisandrin B (-schisandrin, wuweizisu B), schisandrol
B (gomisin A, wuweizi alcohol B, wuweizichun B), schisandrin A (deoxyshisandrin, wuweizisu A), schisantherin A
(gomisin C, wuweizi ester A), and schisantherin B (gomisin B, wuweizi ester B) as the major ones.
3
Several other type of chemicals including daucosterol, 5-hydroxymethyl-2-furancarboxaldehyde, 2-methyl citrate,
protocatechuic acid, quinic acid, thymoquinol 2-glucoside, thymoquinol 5-glucoside, and zingerone glucoside also
were identied in the fruits of Schisandra chinensis.
4

The essential oil of schisandra was found to be composed mainly of terpenes with copaene, alpha-farnesene, and
alpha-cubebene as the major constituents.
5
As the lignans have been proven clinically to be the bioactive components
in schisandra, they are used as chemical markers for quality control.
Schisandrol A: R = OH
Schisandrin A: R = H
Schisandrol B: R = OH
Schisandrin B: R = H

C H 3 O
C H 3 O
C H 3 O
C H 3 O
C H 3 O
C H 3 O
H
R
C H 3
C H 3
C H 3 O
C H 3 O
C H 3 O
C H 3 O
H
R
C H 3
C H 3
O
O
Schisandrol A: R = OH
Schisandrin A: R = H
Schisandrol B: R = OH
Schisandrin B: R = H


C H 3 O
C H 3 O
C H 3 O
C H 3 O
C H 3 O
C H 3 O
H
R
C H 3
C H 3
C H 3 O
C H 3 O
C H 3 O
C H 3 O
H
R
C H 3
C H 3
O
O
Schisandrol A: R = OH
Schisandrin A: R = H
Schisandrol B: R = OH
Schisandrin B: R = H

Four Major Lignans in Schisandra
2 0062 - Schisandra for Schisandrins by HPLC
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Methods of Analysis
Various methods have been used to analyze the lignan content in schisandra, with HPLC being the most acceptable
approach. Most of the published HPLC methods use a methanolwater or acetonitrilewater isocratic mobile
phase and offer good separation, while a gradient mobile phase provides a little better separation. The detection
wavelength was usually 254 nm. As schisandra lignans are fat-soluble compounds, GC is a good alternative.
6
Capillary
electrochromatography and micellar electrokinetic capillary chromatography also have been used to analyze lignans in
schisandra.
7
Extraction is of key importance for accurate analysis of lignans in schisandra. Generally, pure methanol is a good
solvent for extracting samples for analysis.
Method 1:
The method of Bartlova et al.
8
can be used to analyze the six major lignans: schisandrol A, schisandrol B, schisandrin
A, schisandrin B, gomisin N, and schisandrin C. Their method used two chromatographic sets and columns.
Sample Preparation:
Initally, use supercritical uid extraction, then dissolve the samples in methanol for analysis. Or extract 0.5 g of sample
with 95% ethanol by reuxing for 0.5 hour, then dissolve in acetonitrilewater (85:15).
Chromatography:
Column 1: Nucleosil 100, 5 m, C18 endcapped EC 250 4.0 mm.
Mobile phase: Solvent A = water, solvent B = acetonitrile.
Gradient:
Or mobile phase: Solvent A = water, solvent B = methanol.
Column 2: Merck-LiChrospher 100 RP-18, 5 m, 250 4.0 mm.
Mobile phase: Solvent A = water, solvent B = acetonitrile.
Gradient:
Time (minutes) %A %B
0 50 50
5 50 50
35 40 60
55 30 70
70 30 70
Time (minutes) %A %B
0 30 70
1 30 70
35 5 95
40 5 95
Time (minutes) %A %B
0 60 40
60 30 70
90 30 70
Flow rate: 0.75 mL/minute
Injection volume: 20 L
Detection wavelength: 254 nm
Column temperature: Ambient
Flow rate: 0.5 mL/minute
Injection volume: 20 L
Detection wavelength: 254 nm
Column temperature: 25C
3 0062 - Schisandra for Schisandrins by HPLC
10005 Muirlands Blvd., Suite G, Irvine, CA 92618 | Tel: +1-949-419-0288
Fax: +1-949-419-0294 | sales@chromadex.com | www.chromadex.com
2011 ChromaDex, Inc. All rights reserved.
Time (minutes) %A %B
0 55 45
12 40 60
24 40 60
40 10 90
45 10 90
Flow rate: 1.0 mL/minute
Injection volume: 10 L
Detection wavelength: 255 nm
Column temperature: Ambient
Validation Data:
Not available
Method 2:
The method of Wang et al.
3
was used.
Sample Preparation:
Accurately weigh the dried schisandra fruits and fruit extracts (9 to 1) (400 mg for fruit powders and 200 mg for
extract) into a 100-mL volumetric ask. Add 70 mL of methanol and sonicate for 30 minutes, then cool to room
temperature, and ll to volume with methanol.
Chromatography:
Column: Phenomenex Luna C18 (2), 5 m, 250 4.6 mm.
Mobile phase: Solvent A = water (0.1% formic acid), solvent B = acetonitrile.
Gradient:
Representative HPLC Chromatogram of Schisandra Run by Method 2


0

10

20

30

4







Schisandrol A
Schisandrin B

Schisandrol B

Schisandrin A









4 0062 - Schisandra for Schisandrins by HPLC
10005 Muirlands Blvd., Suite G, Irvine, CA 92618 | Tel: +1-949-419-0288
Fax: +1-949-419-0294 | sales@chromadex.com | www.chromadex.com
2011 ChromaDex, Inc. All rights reserved.
References:
1. Chang HM, But PH. Pharmacology and application of Chinese materia medica Singapore: World Science; 1986:1:773.
2. Hanche JL, Burgos RA, Ahumada F. Schisandra chinensis (Turcz.) Baill. Fitoterapia. 1999;70:45171.
3. Wang M, Wu QL, Tadmor Y, et al. Schisandra chinensis: Chemistry and analysis. In: ACS symposium series 859: oriental foods and herbs,
chemistry and health effects. Washington, DC: American Chemical Society; 2003:23446.
4. Dai H, Zhou J, Peng Z, et al. Studies on chemical constituents of Schisandra chinensis. Tianran Chanwu Yanjiu Yu Kaifa. 2001;13(1):246.
5. Qin B, Tian Z, Lou Z. Constituents of the volatile oil from the fruit of Schisandra chinensis. Yaoxue Tongbao. 1988;23(6):3389.
6. Sohn HJ, Bock JY, Baik SO, et al. Determination of lignan compounds in fruits of Schisandra chinensis Baillon by capillary-GC(FID). Hanguk
Nonghwa Hakhoechi. 1989;32(4):3506.
7. Sterbova H, Sevcikova P, Kvasnickova L, et al. Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary
chromatography. Electrophoresis. 2002;23(2):2538.
8. Bartlova M, Opletal L, Chobot V, et al. Liquid chromatographic analysis of supercritical carbon dioxide extracts of Schizandra chinensis. J
Chromatogr B. 2002;770(1-2):2839.