The Extra Expert in your lab:

UV Detector L-2400
UV-Visible Detector L-2420
Diode Array Detector L-2450
Outstanding Performance in HPLC Detection
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0 2 4 6 8 10 12 16 14 18 20 22 24 26 28 30
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10
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0 0.2 0.4 0.6 0.8 1.0 1.2 1.6 1.4 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4
TC = 0.05 s
TC = 2.00 s
350
300
250
200
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200 210 220 230 240 250 260 280 270 290 300
Ratio = 2.75
Perfect UV-Visible Detection for all your needs
New Standards in HPLC
Detection: The L-2400 UV
and L-2420 UV-Visible
Detectors
Both detector types are designed for highest sen-
sitivity by utilising the double beam photometric
detector principle in combination with a grating
monochromator. To improve light intensity and
detection sensitivity in the visible range, the
L-2420 UV-Visible detector contains a tungsten
lamp in addition to the deuterium lamp. Detection
wavelength is time-programmable for optimum
detection sensitivity at the absorbance maximum
of each analyte. If negative peaks should be
recorded and integrated, e.g. applying indirect UV
detection for ion chromatography, the polarity
of the detector signal can be reversed. In
stopped-flow mode spectra of the analytes
can be recorded and background-subtracted for
proper substance identification and selection of
the optimum detection wavelength. Both detec-
tors can be operated either by integration into
the LaChrom Elite system with control through
the EZChrom Elite chromatography data
system or as stand-alone modules via the
optional keypad. In stand-alone mode, several con-
trol functions using contact signals are available.
Highest Sensitivity
and Resolution
Advanced optics and innovative electronics com-
bined in the LaChrom Elite UV-Visible detectors
ensure minimised RI effects, a large linear
dynamic range and superior signal to noise
even with the fastest response times. So lowest
trace level detection limits and better quantitative
reproducibility are achieved. The low baseline
drift makes signal detection insensitive to varia-
tions in ambient conditions and leads to optimum
peak integration. A small optical bandwidth ensures
high spectral resolution of the detectors.
Fit for Fast Chromatography
and Micro Applications
The active noise filter combined with a wide
range of response times, enables optimum
peak data acquisition even in high-speed chro-
matography, e.g. using Chromolith columns.
Narrow, rapidly eluting peaks can be recorded
with the highest signal to noise ratio whilst main-
taining peak shape. For all application ranges, from
micro up to semi-preparative chromatography,
different optional pre-aligned and easy-to-install
flow cells are available.
Extreme spectral resolution of the L-2450 Diode Array Detector
for precise compound identification
m
A
U
Wavelength (mn)
Using the 1 mm slit a maximum resolution
of the benezene spectrum is achieved
m
V
min
Equipping the detectors with a micro flow cell
enables highest sensitivity in trace level micro HPLC
m
A
U
min
Conditions: L-2400 UV detector equipped
with a micro flow cell, L-2100 SMASH pump
Column: Purospher STAR RP-18e 3 m
150-1 mm
Sample: 5 PAH compounds
Eluent: Methanol/water 80/20
Flow rate: 50 l/min
Injection volume: 0.5 l
Wavelength: 254 nm
EZChrom Elite UV detector
setup screen
High-speed chromatography using a Chromolith column at 5 ml/min:
Accurate recording of peak shapes if a time constant of 50 ms is selected
Specifications
UV Detector L-2400
UV-Vis Detector L-2420
Principle Double beam photometric
detector with grating mono-
chromator
Light source L-2400: D
2
lamp
L-2420: D
2
lamp + W lamp
Both: Hg lamp for wavelength
calibration
Wavelength range L-2400: 190-600 nm
L-2420: 190-900 nm
Noise 0.3 x 10
-5
AU
L-2400: at 250 nm
L-2420: at 250 nm and 600 nm
Drift 1.0 x 10
-4
AU/h
typical 0.4 x 10
-4
AU/h
L-2400: at 250 nm
L-2420: at 250 nm and 600 nm
Slit width 6 nm
Response time constant 0.05, 0.1, 0.5, 1, 2, 4, 8 s
Wavelength time program yes
Spectrum scan function with background subtraction
yes
Polarity change yes
GLP functions 1. Automatic wavelength
calibration with different
D
2
lamp and Hg lamp lines
2. Maintenance logbook: D
2
,
W and Hg lamp exchange date,
lamp energy, total lamp-on
time, no. of lamp-on switch-
ings
Additionally with
EZChrom Elite:
- Documentation of instrument
serial no. and configuration
- Comprehensive audit trails
and logbooks
Safety Leak sensor, Error handling
Input/Output contact signals - Auto Zero IN
- Lamp Off IN
- START IN
- BUSY OUT
- ERROR IN/OUT
Power requirements 24 V DC, 100 W
Dimensions 340 (W) x 400 (D) x 150 (H) mm
Diode Array Detector L-2450
Single beam photometric
detector with grating mono-
chromator and 1024 bit diode
array
D
2
+ W lamp
Hg lamp for wavelength
calibration
190-900 nm
0.75 x 10
-5
AU
at 250 nm and 600 nm
0.5 x 10
-3
AU/h
at 250 nm and 600 nm
1 nm, 4 nm
0.15 12 s
by EZChrom Elite
by EZChrom Elite

1. Automatic wavelength
calibration with different
D
2
lamp and Hg lamp lines
2. Maintenance logbook: D
2
,
W and Hg lamp exchange date,
lamp energy, total lamp-on
time, no. of lamp-on switch-
ings
Additionally with
EZChrom Elite:
- Documentation of instrument
serial no. and configuration
- Comprehensive audit trails
and logbooks
Leak sensor, Error handling
- START IN
- BUSY OUT
- ERROR IN / OUT
24 V DC, 100 W
340 (W) x 400 (D) x 150 (H) mm
The LaChrom Elite Diode Array Detector is
designed as a single beam photometric detector
with a grating monochromator. It is equipped
with a 1024 bit diode array element to sup-
ply the highest spectral resolution over the
complete wavelength range. For light sources the
detector contains both a deuterium and a
tungsten lamp to ensure maximum sensitivity
even in the visible range. The selectable optical
slit width and electronic diode bunching opti-
mise spectral resolution and sensitivity as
required for each specific analysis. The setting of
the electronic noise filter is coupled to the data
acquisition period. This effectively suppresses
noise whilst at the same time accurately
records peak shapes, particularly fast eluting
peaks in high-speed chromatography.
3-dimensional chromatographic data is recorded
and processed in combination with the EZChrom
Elite chromatography data system.
The following advanced 3D functions
are available:

3D chromatogram data can be background-

subtracted and displayed as contour plot,
as 3D chromatograms, as extracted
2D chromatograms and extracted spectra.
Combinations of these display modes can also
be selected.

Automatic peak identification by compari-

son of analyte spectra with library spectra.

Peak purity assessment by comparison of

spectra taken at different positions of the
peak.

Comparison of spectra and calculation of

similarity indices.

Hit list of best fitting spectra with similari-

ty indices.

Max Plot: Chromatogram at absorbance

maximum for each analyte.

Wavelength bunching: Chromatogram

calculated over a specified wavelength range
for optimising sensitivity.

Ratio Plot: Ratio chromatogram calculated

from chromatograms at two different wave-
lengths for assessing peak purity.
Mixed View screen of EZChrom Elite
with diode array detector data
Selection of contour plot, spectra,
2D chromatogram and 3D chromatogram
W lamp
Optics of the L-2450 Diode Array Detector
showing the Hg lamp for automatic
wavelength calibration
3D-Chromatography with the Diode Array Detector L-2450
Peak Identification and Peak Purity Assessment at Lowest Concentration Levels
Flow cell
Hg lamp
Slit
Grating monochromator
Photodiode array
D
2
lamp
Ordering Information
VWR International GmbH Scientific Instruments
Hilpertstrae 20A D-64295 Darmstadt
Fax: +49- 6151- 3972-101
www.vwr.com chrom-instruments@de.vwr.com
Designation
L-2400 UV Detector
L-2420 UV-Vis Detector
L-2450 Diode Array Detector
USB Interface board
UI Pad kit for UV or UV/Vis Detector
Power supply: 100 W AC Adaptor
Organiser
(System power supply and solvent tray)
Analog signal output
for UV or UV/Vis Detector
Analog signal output for DAD (2 channels)
e-DIO cable
for use of START/BUSY/ERROR
contact signals in stand-alone mode
System adaptation kits
for micro and semi-micro applications
Cat. No.
890-0402
890-0422
890-0452
890-0702
890-0740
890-0872
890-0852
890-0510
890-0560
890-6145
On request
Designation
Standard flow cell
(10 mm, 13 l)
Semi-micro flow cell
(5 mm, 3.2 l)
Micro flow cell (5 mm, 0.9 l)
Chromolith cell (10 mm, 13 l)
High-pressure semi-micro
flow cell
(5 mm, 3.2 l, 150 bar)
Cuvette holder unit
L-2450 DAD
Cat. No.
890-0550
890-0554
890-0556
use std. cell
890-0555
890-0558
Convenient Maintenance
All maintenance parts are accessible from the
front of the instruments. Therefore, the user can
easily carry out exchange of the detector lamps and
cleaning or exchange of the flow cell.
FDA, GMP, GLP functions included
All detector types are equipped with a mercury lamp for automatic wavelength calibra-
tion. Using the different spectral lines of deuterium and mercury lamps, wavelength calibration
can be performed over the whole spectral range of the detectors. In contrast to calibration
filters (e.g. Holmium oxide filters), which have to be recalibrated themselves periodically, the
spectral lines of the lamps remain completely constant with time and need not to be recali-
brated. On the other hand, for wavelength and absorbance calibration with standard solutions
according to the European Pharmacopoeia, an optional cuvette holder can be supplied.
The detectors contain a maintenance logbook, which records the exchange date of the
lamps, the lamp energy, the total lamp on time and the number of lamp on switchings.
This facilitates regular maintenance and essential documentation. In combination with the
EZChrom Elite chromatography data system, the complete range of GxP-related
functions becomes available e.g., recording of the instrument serial number, hardware
logbooks and audit trails, automatic documentation of the instrument parameters, etc..
When connected to EZChrom Elite software, full compliance with the FDA rule 21CFR Part
11 is achieved. Moreover, module qualification (OQ) can be substantially facilitated using
the optional Auto-Validation software.
Perfect UV or UV-Visible Detectors for all your needs
LaChrom

and LaChrom Elite

are registered trademarks of VWR International, EZChrom Elite

is a registered trademark of Scientific Software Inc.,

Chromolith

and Purospher

are registered trademarks of Merck KGaA. All other product or company names are trademarks of their respective owners.
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L-2400 UV
L-2420 UV-Vis
Cat. No.
890-0500
890-0504
890-0506
890-0509
890-0505
890-0508
Optional Flow Cells for
Automatic wavelength accuracy
check using different spectral lines
of the deuterium and
mercury lamps
UV Detector L-2400 UV-Visible Detector L-2420 Diode Array Detector L-2450