Abstract Book

ABSTRACT
BOOK
ABSTRACT
BOOK
ABSTRACT
BOOK
ABSTRACT
BOOK
st
1 Annual Conference of
Association of Pharmaceutical Teachers of India
Haryana State Branch
PHARMACEUTICS
S. NO. PAPER
CODE
AUTHOR TITLE
1. PCEU-2 PAARAS GUPTA SOLID DISPERSION FAST DISSOLVING TABLETS OF GLIMEPIRIDE
2. PCEU-3 IPSHITA MENON PHOSPHOLIPID AND MACROGOL BASED MIXED NANOMICELLES ENHANCE
ANTIOXIDANT STATUS OF MELOXICAM
3. PCEU-4 ANKITA KAPOOR HYDROPHOBIC DRUG KETOPROFEN IN TOPICAL EMULGEL
4. PCEU-5 AKSHAY SHAH FORMULATION AND EVALUATION OF NANOSIZED ETHOSOMES FOR ENHANCED
TRANSDERMAL DELIVERY OF KETOROLAC TROMETHAMINE
5. PCEU-6 MAHMOOD SHAIKH OPTIMIZATION AND CHARACTERIZATION OF NOVEL ALGINATE-CHITOSAN
NANOPARTICLES CONTAINING PAYLOAD OF ANTI TUBERCULAR DRUG
CYCLOSERINE
6. PCEU-7 SUSHIL NAGAR MICROBIALLY SYNTHESIZED HIGH-QUALITY NANOSTRUCTURES FOR DRUG
DELIVERY SYSTEMS: A GREEN SYNTHESIS APPROACH
7. PCEU-10 SUSHAMA
TALEGAONKAR
LONG CIRCULATING SURFACE DECORATED LIPOSOMES FOR EFFICIENT
TREATMENT OF MDR CANCER
8. PCEU-11 PRIYANKA VERMA EFFECT OF POLYMERS ON SOLID DISPERSIONS OF ACECLOFENAC
9. PCEU-13 LOKESHSADANA NATURAL POLYMERS BASEDMAGNETIC MICROSPHERES AS A EFFECTIVE TOOL
FOR CHRONIC DISEASE
10. PCEU-14 RAJNI BALA CARBON NANOTUBES AS A DRUG DELIEVERY SYSTEM: A REVIEW
11. PCEU-15 BHUPINDER KAUR FABRICATION AND EVALUATION OF ORO-DISPERSIBLE PECTIN-HPMCE15LV FILMS
OF PROPANOLOL HYDROCHLORIDE
12. PCEU-16 JATINDER KUMAR CHRONOTHERAPEUTIC DRUG DELIVERY OF PECTIN, GUAR GUM AND OKRA GUM
CONTROLLED RELEASE COLON TARGETED DIRECTLY COMPRESSED
PROPRANOLOL HCL MATRIX TABLETS AND IN-VITRO EVALUATION
13. PCEU-17 MANDEEP SINGH FORMULATION AND EVALUATION OF ORO-DISPERSIBLE TABLETS FOR ANTI-
DEPRESSENT DRUG CITALOPRAM HYDROBROMIDE
14. PCEU-18 NARINDER KUMAR FORMULATION AND DEVELOPMENT OF ONDANSETRON MOUTH DISSOLVING
TABLETS BY USING STARCH CITRATE VS CONVENTIONAL SUPERDISINTEGRANTS
AND IN-VITRO EVALUATION
15. PCEU-19 SANDEEP KAUR A STUDY ON HPMC K4M AND CARBOPOL 940 BASED AMOXICILLIN TRIHYDRATE
FLOATING TABLETS AND IN VITRO EVALUATION FOR THE TREATMENT OF
H.PYLORI INFECTION
16. PCEU-20 TEJPAL SINGH IMPROVEMENT OF DISSOLUTION PROFILES OF WATER INSOLUBLE DRUG
ITRACONAZOLE USING CRYSTALLINE AND AMORPHOUS CARRIER
17. PCEU-21 GARIMA SINGH SOLID SMEDDS AS POTENTIAL CARRIER FOR NOVEL DRUG DELIVERY SYSTEMS
18. PCEU-22 PRIYA RANI APPLICATIONS OF MICROWAVE TECHNOLOGY FOR POLYMER GRAFTING
19. PCEU-23 GURPREET KAUR PREPARATION AND EVALUATION OF FLOATING TABLETS OF AN
ANTIHYPERTENSIVE DRUG
20. PCEU-24 PRIYANKA YADAV FORMULATION AND EVALUATION OF GASTRORETENTIVE TABLETS USING
COMBINATION OF HYDROPHILIC AND HYDROPHOBIC POLYMERS
21. PCEU-25 AJAY SAROHA DEVELOPMENT AND EVALUATION OF TIMOLOL MALEATE LOADED CHITOSAN
NANOPATICLES FOR OCULAR DELIVERY
22. PCEU-26 ISHA DESIGN AND EVALUATION OF EFFERVESCENT FLOATING TABLETS OF
CEFUROXIME AXETIL
23. PCEU-27 MANIK GOEL MULTIFUNCTIONALITY OF NANO-PARTICLES AS CARRIERS FOR VARIOUS
THERAPIES
24. PCEU-28 PRITI GIROTRA DESIGN AND DEVELOPMENT OF SILYMARIN NANOPARTICLES FOR ENHANCED
HEPATOPROTECTIVE ACTIVITY
25. PCEU-29 NANCY ACCELERATED STABILITY STUDY OF SHANKHPUSHPI AND BRAHMI SYRUP
26. PCEU-30 RAMIKA HARDATT INVESTIGATIONS ON SOLUBILITY ENHANCEMENT VIA SOLID DISPERSIONS USING
MODIFIED NOVEL CARRIERS
27. PCEU-31 DIVYA SELF EMULSIFYING SOLUTIONS : IMPROVING BIOAVAILABILITY OF BCS CLASS II
DRUGS
28. PCEU-33 NEHA KHAR PRONIOSOMES:-A PREFERABLE CARRIER FOR DRUG DELIVERY SYSTEM
29. PCEU-34 RAMI RANI FORCED DEGRADATION STUDIES ON AZILSARTAN
30. PCEU-35 VANEETPAL
KHURANA
FORCED DEGRADATION STUDIES ON FLUPIRITINE MALEATE
31. PCEU-36 ANKITA
BHARDWAJ
DIFFERENT METHODS OF ENHANCEMENT OF SOLUBILIZATION AND
BIOAVAILABILITY OF POORLY SOLUBLE DRUGS
32. PCEU-37 NEETA NANOTECHNOLOGY FOR CANCER TREATMENT
33. PCEU-39 PRIYA NANOTECHNOLOGY AS A THERAPEUTIC TOOL TOCOMBAT MICROBIAL
RESISTANCE
34. PCEU-40 DAISY ARORA INSULIN LOADED BILOSOMES FOR TREATMENT OF DIABETES MELLITUS
35. PCEU-41 BHARAT KHURANA DEVELOPMENT AND CHARACTERIZATION OF AMPHOTERICIN B LOADED
ETHOSOMES FOR THE ENHANCED TREATMENT OF TOPICAL FUNGAL INFECTIONS
36. PCEU-42 GURMEET SINGH DEGRADATION PRODUCT PROFILING OF HYDROXYCHLOROQUINE USING MS
N
, LC-
ESI-MS-TOF AND LC-PDA TECHNIQUES
37. PCEU-43 GOURAV NANOPARTICLES FOR DRUG DELIVERY TO THE BRAIN
38. PCEU-44 S.K.GUPTA FORMULATION AND EVALUATION OF ENTERIC COATED MULTIPARTICULATE
SYSTEMS OF ESOMEPRAZOLE MAGNESIUM
39. PCEU-45 S.K.GUPTA QUALITY AND PERFORMANCE ENHANCEMENT OF NSAIDS ANTIPLATELET AGENT
IN GASTRORESISTANT FORMULATION
40. PCEU-46 JASMINE NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL
AND PROMETHAZINE IN SYRUP FORMULATIONS.
41. PCEU-47 KOMAL NANOPARTICLES: NOVEL DRUG DELIVERY SYSTEM FOR CANCER
42. PCEU-48 RAJNI DEVI TRANSDERMAL DRUG DELIVERY SYSTEM
43. PCEU-49 MOHINI SHARMA NATURAL THERAPY BASED ON NATURAL POLYMERS USED FOR COLONIC
DISEASES
44. PCEU-50 ASHISH SINGLE DEVELOPMENT & CHARACTERIZATION OF LOSARTAN LOADED FLOATING
GASTRORETENTIVE MICROSPHERES
45. PCEU-51 SWATI AGGARWAL AN OVERVIEW ON SELF-ASSEMBLED NANOPARTICULATE CARRIER SYSTEM:
AQUASOMES
46. PCEU-52 DIVYA BHATIA SCREENING OF DIFFERENT GROWTH MEDIUM FOR EXTRACELLULAR BACTERIAL
SYNTHESIS OF SILVER NANOPARTICLES: NEW METHOD
47. PCEU-53 ANSHUL GARG DENDRIMERS : A PROMISING NANODEVICES FOR TARGETED DRUG DELIVERY
48. PCEU-54 YUGUM TANEJA NIOSOMES: A POTENTIAL VESICULAR DRUG DELIVERY SYSTEM
49. PCEU-55 SWATI KODAN ANTIMICROBIAL ACTIVITY OF GREEN SYNTHESIS SILVER NANOPARTICLES
AGAINST HUMAN PATHOGENS
50. PCEU-56 PARVEEN KUMAR NEW HORIZONS IN THE VISTAS OF MICROEMULSIONS FOR IMPROVED
DRUG DELIVERY
51. PCEU-57 PARIJAT PANDEY NANOPARTICLES-IN-MICROPARTICLES SYSTEMS: AN OVERVIEW
52. PCEU-59 HIMANSHU ROLE OF TASTE AND TECHNIQUS OF TASTE MASKING OF BITTER DRUG
53. PCEU-60 KOMAL GOSWAMI FORMULATION AND EVALUATION OF LEVOFLOXACIN SUSTAINED RELEASE
TABLET
54. PCEU-61 NEELAM POONIA PREPARATION AND CHARACTERIZATION OF NANOSTRUCTURED LIPID CARRIERS
BASED TOPICAL DELIVERY OF DICLOFENAC
55. PCEU-62 ATUL ATTRI TARGETED DRUG DELIVERY SYSTEM
56. PCEU-63 POKALI
SARABAIAH
FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY SYSTEM FOR
DOMPERIDONE
57. PCEU-64 PRITI
MEHNDIRATTA
FORMULATION AND EVALUATION OF IMMEDIATE RELEASE TABLET OF
AMLODIPINE
58. PCEU-65 RAHUL RATHEE MICROPARTICULATE DRUG DELIVERY SYSTEM
59. PCEU-66 UPMA FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY SYSTEM FOR
FAMOTIDINE
60. PCEU-67 SANDEEP KUMAR TARGETING IMMUNE SYSTEM BY NANOPARTICLES TO COMBAT CANCER
61. PCEU-68 RAJESH KUMAR TRANSFERSOME: A NOVEL TRANSDERMAL CARRIER SYSTEM
62. PCEU-69 JYOTI DAHIYA GASTRIC RETENTION: AN APPROACH TO ORAL CONTROLLED DRUG DELIVERY
SYSTEM
63. PCEU-70 H. S. CHAWDA GASTRORETENTIVE DRUG DELIVERY SYSTEM: A POTENTIAL APPROACH FOR
GASTRIC RETENTION
64. PCEU-71 MONA PIPLANI TARGETED PRODRUG STRATEGY FOR OPTIMIZED DRUG DELIVERY
65. PCEU-72 ARCHANA
KAUSHIK
FORMULATION AND EVALUATION OF SILYBUM MARIANUM LOADED
MICROSPONGES
66. PCEU-73 BINKATESH
KUMAR
A REVIEW: FLOATING DRUG DELIVERY SYSTEM
67. PCEU-74 SARIT DHIMAN NOVEL TRENDS IN PULSATILE DRUG DELIVERY TECHNOLOGY
68. PCEU-76 HIMMAT SINGH FORMULATION & EVALUATION OF NIMESULIDE ENTRAPPED NIOSOMES
69. PCEU-78 VIPUL GOGAR FORMULATION OF SUSTAINED RELEASE MATRIX TABLET USINGHYDROPLILIC
AND HYDROPHOBIC POLYMERS
70. PCEU-79 KIRAN YADAV RECENT ADVANCES AND NOVEL STRATEGIES IN DEVELOPMENT OF
NANOPARTICLE BASED THERANOSTICS
71. PCEU-80 RAMNARESH COMPARATIVE BIOAVAILABILITY STUDY OF KETOPROFEN PREPARED BY SOLID
UNIYAL DISPERSION, BETA-CYCLODEXTRIN COPLEXATION AND SOLID SMEDDS
72. PCEU-81 SANDEEP KUMAR FORMULATION AND IN-VITRO EVALUATION OF ORALLY ADMINISTERED GASTRO
RETENTIVE FLOATING TABLETS OF SIMVASTATIN
73. PCEU-82 NIKHIL SINGH FORMULATION AND EVALUATION OF IMMEDIATE REALEASE TABLET OF
OLMESARTAN HYDROCHLOROTHIAZIDE USING WET GRANULATION METHOD
74. PCEU-83 SANJAY KUMAR
PANDEY
FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL TABLETS
CONTAINING ANTI MIGRANT AGENT
75. PCEU-84 NARESH KALRA SENSITIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE
DETERMINATION OF TAXOL CATEGORY DRUG IN PLASMA.
76. PCEU-85 SHASHIKANT
BHATT
FORMULATION AND EVALUATION OF RESPERIDONE LOADED NANOPARTICLES
77. PCEU-86 UDAY PRAKASH
MISHRA
COMPARATIVE STUDY AS FORMULATION AND EVALUATION OF VARIOUS TASTE
MASKING COMPLEXES OF ROXITHROMYCIN DISPERSIBLE TABLET
78. PCEU-87 ABHINAV SINGH
RANA
A REVIEW ON INSOLUBLE DRUG DELIVERY TECHNOLOGY
79. PCEU-88 PRIYA RANI APPLICATIONS OF MICROWAVE TECHNOLOGY FOR POLYMER GRAFTING
80. PCEU-89 SURENDRA
BHAMBU
SUSTAINED RELEASE MATRIX TECHNOLOGY AND RECENT ADVANCE IN MATRIX
DRUG DELIVERY SYSTEM
81. PCEU-90 BHASKAR BARSAR CONTROLLED RELEASE ION EXCHANGE RESIN DRUG DELIVERY SYSTEM AND
RECENT DEVELOPMENT IN ION EXCHANGE RESIN DELIVERY SYSTEM
82. PCEU-91 SHREERAM
BANGARWA
DEVELOPMENT AND CHARACTERIZATION OF ANTIFUNGAL GEL OF
CLOTRIMAZOLE
83. PCEU-92 SOHAN LAL MATRIX TABLETS: AN APPROACH TOWARDS ORAL EXTENDED RELEASE DRUG
DELIVERY
84. PCEU-93 SHUBHANGI
CHAUHAN
MITOCHONDRIA TARGETTED ANTIOXIDANTS
85. PCEU-94 DEEPU PURI SUSTAINED RELEASE MATRIX TECHNOLOGY AND RECENT ADVANCE IN
MATRIX DRUG DELIVERY
SYSTEM
86. PCEU-95 DEEPIKA
AGGARWAL
SYNTHESIS AND CHARACTERIZATION OF SILVER AND GUAR GUM
NANOPARTICLES
87. PCEU-96 SUDHIR KUMAR STUDY OF DISSOLUTION RATE PROFILE OF MODIFIED RELEASE PELLETS
CONTAINING VENLAFAXINE HYDROCHLORIDE
88. PCEU-97 PRIYANKA
KRIPLANI
CHRONOPHARMACEUTICS - A NOVEL APPROACH FOR DRUG DELIVERY

PHARMACOLOGY
S. NO PAPER
CODE
AUTHOR TITLE
1. PCL-1 PAWAN KAUSHIK ISOLATION, CHARACTERIZATION AND -AMYLASE ACTIVITY OF QUERCITIN
FROM PINUSROXBURGHII
2. PCL-2 RITU MAHAJAN ANTIMICROBIAL POTENTIAL OF VARIOUS LEAF EXTRACTS OF BLUMEASPECIES
3. PCL-3 MOHIT DRUG-RESISTANT MALARIA IN SOUTH ASIAN COUNTRIES: A REVIEW OF
EVIDENCE AND FUTURE PROSPECTS OF NANOMEDICINE BASED STRATEGIES FOR
PROPHYLAXIS AND TREATMENT
4. PCL-4 ASHOK JANGRA SODIUM PHENYLBUTYRATE, A HISTONE DEACETYLASE INHIBITOR, PROTECTS
AGAINST CHRONIC ETHANOL-INDUCED COGNITIVE DYSFUNCTION AND
ALTERATION IN HIPPOCAMPAL BNDF EXPRESSION LEVEL
5. PCL-6 ANITA DUA INDIAN MUSTARD (BRASSICA JUNCEA) REMNANT AFTER OIL EXTRACTION
PROTECT BIOMOLECULES AGAINSTINVITRO OXIDATION
6. PCL-7 KARTIK SHARMA ANTI-ULCER ACTIVITY OF DEGLYCYRRHIZINIZEDLIQUORICE
7. PCL-8 PARUL POSSIBLE PROTECTIVE EFFECT OF PPAR-ALPHA AGONIST AND CCBS AGAINST 3-
NP INDUCED HD
8. PCL-9 RUPINDER KAUR EFFECT OF GINSENG AGAINST HALOPERIDOL INDUCED OROFACIAL DYSKINESIA
9. PCL-10 UMA JYOTI EFFECT OF DPP-IV INHIBITOR ON EXPERIMENTAL ENDOTHELIAL DYSFUNCTION
10. PCL-11 GURMEETKAUR EFFECT OF PROGESTERONE AGAINST EXPERIMENTAL PARKINSONS DISEASE
11. PCL-12 ARSHVIRKAUR EFFECT OF ETHYL ACETATE STEM BARKFRACTION OF BETULAALNOIDES
AGAINST MPTP- INDUCED EXPERIMENTAL PARKINSON'S DISEASE IN RAT
12. PCL-13 GANESHSINGH
BHAKUNI
HEPATOPROTECTIVE EFFECT HERBAL DRUGS AGAINST HIGH FAT DIET AND
ALCOHOL INDUCE HEPATOTOXICITY IN RATS: POSSIBLE SYNERGISTIC EFFECT
13. PCL-14 NAVNEETKAUR ROLE OF HEMEOXYGENASE-1/GLYCOGEN SYNTHASE KINASE-3 PATHWAY IN 3-
NITROPROPIONIC ACID INDUCED NEUROTOXICITY IN RATS
14. PCL-15 PRIYAJASWAL FRACTIONS OF BUTEAMONOSPERMA LEAF ATTENUATE MPTP-INDUCED
BEHAVIORAL MOTOR DEFICIT AND OXIDATIVE STRESS IN RATS
15. PCL-16 SWATI DATTA POSSIBLE BENEFICIAL EFFECT OF PEROXISOME PROLIFERATOR-ACTIVATED
RECEPTOR (PPAR) - AND AGONIST AGAINST A RAT MODEL OF ORAL
DYSKINESIA
16. PCL-17 TAVLEENKAUR ROLE OF FXR/H2S PATHWAY IN DINITRO BENZENE SULFONIC ACID (DNBS) -
INDUCED ULCERATIVE COLITIS IN RATS
17. PCL-18 VANDANA BENEFICIAL EFFECT OF SPHINGOSINE-1-PHOSPHATE RECEPTOR ANALOG AS ANTI-
ARTHRITIC AND ANTI-OSTEOPOROTIC IN CFA INDUCED OVARIECTOMIZED RATS
18. PCL-19 PARAMDEEP SINGH EVALUATION OF THE ANTI-INFLAMMATORY PROPERTY OF COMBINED THERAPY
OF CHOLINERGIC ANTAGONIST SCOPOLAMINE AND VENLAFAXINE IN RATS.
19. PCL-20 ANKUR GERA REVIVAL OF THALIDOMIDE TRANSFORMS IT INTO A VERSATILE NOVEL
THERAPEUTIC AGENT
20. PCL-21 KAPIL SWATI SLEEPAPNOEA:LIFE THREATENING BUT LIMITED AWARENESS
21. PCL-22 DEEPIKADEOPA DIABETIC WOUND CARE AND MANAGEMENT
22. PCL-23 JASMINA AUTOIMMUNE DISORDERS AND ALTERNATE THERAPIES
23. PCL-24 SIDDHARTH
SHARMA
SIGMA RECEPTORS- A MILESTONE IN THE TREATMENT OF NEUROPSYCHIATRIC
DISORDERS
24. PCL-25 SUMIT SACHDEVA ETHNO-PHARMACOLOGICALLY USED PLANTS FOR MALARIA TREATMENT IN
NORTHERN INDIA
25. PCL-26 RAHUL GUPTA NUTRACEUTICALS ANTIOXIDANTS EFFECTS ON PESTICIDE TOXICITY STUDIES
26. PCL-27 ANKUR GARG DIETING IN OBESITY: IS IT REALLY EFFECTIVE?
27. PCL-28 AMANDEEP THAKUR CHEMOTHERAPY AND IMMUNOTHERAPY FOR TUBERCULOSIS
28. PCL-29 PRIYA JASWAL PARKINSONISM- A NEURODEGENERATIVE DISORDER
29. PCL-30 RIYA THAKUR GOUT: A COMMON FORM OF ARTHRITIS
30. PCL-31 SHIV KANT SHARMA HISTOLOGICAL STUDY OF MALATHION TREATED INTESTINE IN WISTAR RATS
31. PCL-32 NEERAJ GILHOTRA ELEVATED TUNNEL MAZE : INDIAS OWN MAZE FOR MEASUREMENT OF ANXIETY
32. PCL-33 SEZAL DIMINISHED ANTI-ANXIETY EFFECT OF DIAZEPAM IN STRESSED MICE UNDER
INFLUENCE OF P38MAPKINASE
33. PCL-34 AJMER SINGH ALDOSE REDUCTASE INHIBITORS FOR MANAGEMENT OF DIABETIC
GREWAL COMPLICATIONS
34. PCL-35 BHAWNA VAISH DOTS THERAPY FOR TUBERCULOSIS
35. PCL-36 MINAKSHI GUPTA ADVANCED THERAPIES IN CANCER TREATMENT: AN OVERVIEW
36. PCL-37 SEEMA CHOKAR POSSIBLE UNDERLYING INFLUENCE OF NF-B IN THE DIMINISHED ANTI-ANXIETY
EFFECT OF DIAZEPAM IN STRESSED MICE
37. PCL-38 SANT LAL DIFFERENTIAL MODULATION BY NEUROCHEMICALS OF ANXIETY DISORDERS
38. PCL-39 SANDEEP KAUR DRUG INDUCED HEPATOXICITY
39. PCL-40 OJASHVI SHARMA RHEUMATOID ARTHRITIS CAUSES AND TREATMENT: REVIEW
40. PCL-41 ABHISHEK DABRA PATHOGENESIS OF PEPTIC ULCER : A REVIEW
41. PCL-42 PRIYANKA PAHWA MEMORY ENHANCING POTENTIAL OF POLYHERBAL FORMULATION: PLAUSIBLE
ROLE OF OXIDATIVE BIOMARKERS
42. PCL-43 NEETU STUDIES TO EXPLORE A COMPREHENSIVE POTENTIAL OF AGMATINE FOR THE
TREATMENT EPILEPSY AND ASSOCIATED COMORBIDITIES
43. PCL-44 NAVJOT KAUR COMPARATIVE ANALYSIS OF EPILEPTIC BEHAVIOURAL CO-MORBIDITIES IN
PENTYLENETETRAZOLE KINDLED AND KINDLING RESISTANT MICE.
44. PCL-45 VINESH MULTIPLE EXPOSURE TO DICHLORVOS AND MONOCROTOPHOS ON THE
BREATHING PATTERN AND RESPIRATORY VARIABLES IN RATS
45. PCL-46 SATBIR KAUR BOTTLE GOURD IS A NAURAL GUARD
46. PCL-47 KULDEEP VYAS RNA INTERFERENCE AND PERSONALIZED CANCER THERAPY
PHARMACEUTICAL CHEMISTRY
S. NO PAPER
CODE
AUTHOR TITLE
1. PCHEM-1 SAMRIDHI SYNTHESIS AND BIOLOGICAL EVALUATION OF 3-(4-(2-(ARYL)-4-OXOTHIAZOLIDIN-
3-YL)PHENYL-2-PHENYLQUINAZOLIN-4(3H)-ONES
2. PCHEM-4 RUCHIKA GOYAL DESIGN, SYNTHESIS AND EVALUATION OF CHALCONE-THIAZOLIDINONE HYBRIDS
FOR ANTICANCER ACTIVITY
3. PCHEM-5 SONIA KOHLI CHROMONEDERIVATIVES : AS ANTIDEPRESSANT AGENTS
4. PCHEM-6 NAVIDHA SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF NEW SUBSTITUTED 2-(4-(5-
ARYLISOXAZOL-3-YL)PHENOXY)ACETIC ACID DERIVATIVES
5. PCHEM-7 MANISHA BISHNOI SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 2-(CHLOROMETHYL)-4-
PHENYLQUINOLINE-3-CARBOHYDRAZIDE DERIVATIVES
6. PCHEM-8 DEEPIKA PUROHIT 3D QSAR AND DOCKING STUDY OF 2-((PYRIDIN-3-YLOXY) METHYL)PIPERAZINES
AS 7 NICOTINIC ACETYLCHOLINE RECEPTOR MODULATORS FOR THE
TREATMENT OF INFLAMMATORY DISORDERS
7. PCHEM-9 MUKIL KUMAR SYNTHESIS AND ANTIMICROBIAL SCREENING OF N-(2-(ARYL)-4-OXOTHIAZOLIDIN-
3-YL)-2-(NAPTHALEN-2-YLOXY)PROPANAMIDES
8. PCHEM-
10
ANSHU PRABHA
SINGH
SYNTHESIS AND EVALUATION OF SOME 5-(4-((2-SUBSTITUTED-4-OXOQUINAZOLIN-
3(4H)-YL)IMINO)METHYLBENZYLIDENE)-1,3-THIAZOLIDINE-2,4-DIONE
DERIVATIVES FOR POTENTIAL ANTIMICROBIAL ACTIVITY
9. PCHEM-
11
ABHISHEK SYNTHESIS AND ANTIMICROBIAL EVALUATION OF N-(2-(ARYL)-4-
OXOTHIAZOLIDIN-3-YL)-2-(2-(2-METHYL-5-NITRO-1H-IMIDAZOL-1-
YL)ETHOXY)ACETAMIDE
10. PCHEM-
12
SWARANDEEP
KOHLI
SYNTHESIS AND PHARMACOLOGICAL EVALAUTION OF BENZOXAZOLE-
COUMARIN DERIVATIVES AS SAFE ANTI-INLFAMMATORY AGENTS
11. PCHEM-
13
BABITA DEVI HYDROGEN SULFIDE RELEASING HYBRIDS A NOVEL APPROACH
12. PCHEM-
14
DEEPIKA PALIWAL DESIGN, MOLECULAR DOCKING AND SYNTHESIS OF 2-(2-HYDRAZINYL)THIAZOLE
DERIVATIVES AS POTENTIAL ANTI-MALARIAL AGENTS
13. PCHEM-
15
PRADEEP KUMAR HANSCH ANALYSIS OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF
SUBSTITUTED 1-ALKYL/ARYL-3-ETHOXY CARBONYL-5-HYDROXY-2-METHYL
INDOLES
14. PCHEM-
16
DEEPIKA SAINI DESIGN AND SYNTHESIS OF QUINOLINYL PYRAZOLES AS DUAL INHIBITOR OF
FALCIPAIN-2 & DIHYDROFOLATE REDUCTASE FOR ANTIMALARIAL POTENTIAL
15. PCHEM-
17
AMAN THAKUR THE DISCOVERY OF LESS TOXIC NOVEL DPP-IV INHIBITORS: PHARMACOPHORE
MODELING, VIRTUAL SCREENING, MOLECULAR DOCKING AND IN SILICO ADMET
STUDIES
16. PCHEM-
18
DEEPIKA
CHAUDARY
MOLECULAR MODELING AND ADMET PREDICTION STUDIES OF NOVEL
BUTENOLIDES AS POTENTIAL INHIBITOR OF PLASMODIUM FALCIPARUM L-
LACTATE DEHYDROGENASE
17. PCHEM-
19
SANJIV KUMAR SYNTHESIS, ANTIMICROBIAL AND ANTICANCER EVALUATION OF N-[2-(4-CHLORO-
PHENYL)-4-OXO-4H-QUINAZOLIN-3-YL]-2-{4-[2-(SUBSTITUTED PHENYLAMINO)-
ACETYL]-PIPERAZIN-1-YL}-ACETAMIDE DERIVATIVES
18. PCHEM-
20
SAVITRI DESIGN, SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 1-AMINO-1, 8A-
DIHYDROQUINOLINE-2(4AH)-ONE DERIVATIVE
19. PCHEM-
21
MANISHA DESIGN, SYNTHESIS AND ANTIMICROBIAL EVALUATION OF N-SUBSTITUED-2-(1H-
BENZOTRIAZOL-1-YL) ACETOHYDRAZIDE DERIVATIVES
20. PCHEM-
22
SONAKSHI SETH THIAZOLIDINONES: THE VERSATILE PHARMACOPHORE OF MEDICINAL
SIGNIFICANCE
21. PCHEM-
23
ARCHANA SHARMA SYNTHESIS OF NOVEL IMIDAZOLE CLUBBED TRIAZOLE DERIVATIVES
22. PCHEM-
24
RITIKA BHATIA SYNTHESIS AND ANTIMICROBIAL EVALUATION OF AMINO-BIPHENYL-3-
CARBALDEHYDE DERIVATIVES
23. PCHEM-
25
MANJU RANI QUINOLINE AS ANTIMALARIAL AGENTS: A REVIEW
24. PCHEM-
26
USHA RANI PYRAZOLE AND ITS DERIVATIVES OF ANTI-DIABETIC POTENTIAL: A REVIEW
25. PCHEM-
27
VIKAS SHARMA PYRIDOACRIDINES AS NATURAL ANTICANCER LEAD COMPOUNDS: A REVIEW
26. PCHEM-
28
PANKAJ SARASWAT SYNTHESIS AND DISCOVERY OF NOVEL DISPIRO COMPOUNDS AS INHIBITORS OF
ACETYLCHOLINESTERASE.
27. PCHEM-
29
MOHD. ZAHEEN
HASSAN
SYNTHESIS AND BIOLOGICAL EVALUATION OF PYRIMIDINE ANALOGUES AS
ANTICONVULSANT AGENTS
28. PCHEM-
30
VEERENDRA SAINI DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF NEWER PYRAZOLINE
ANALOGUE
29. PCHEM-
31
KAVITA
CHOUDHARY
DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF NEWER PYRAZOLE
ANALOGUE

PHARMACOGNOSY
S. NO PAPER
CODE
AUTHOR TITLE
1. PCOG-1 GAURAV GUPTA STUDIES OF ANXIOLYTIC EFFECTS OF AN AYURVEDIC NANOMEDICINE AKIK
BHASMA
2. PCOG-2 LOVEDEEP KUMAR EFFECT OF SUCCESSIVE LEAF EXTRACTS OF BASELLA ALBA IN GASTRIC ULCER
3. PCOG-3 DHRUV
SABHARWAL
MICROWAVE- ASSISTED EXTRACTION: NOVEL TECHNIQUE FOR ISOLATION AND
EXTRACTION OF PHYTOCONSTITUENTS
4. PCOG-4 JASKARAN SINGH FLORASOLS/PHYTONIC EXTRACTION: AN ADVANCED TECHNIQUE
5. PCOG-5 ANDLEEB KAUR SEPERATION AND QUALITATIVE DETERMINATION OF FLAVONOIDS FROM TETLEY
TEA.
6. PCOG-6 SONALI BATRA MAHANIMBIN: ANTIANXIETY AND ANTIDEPRESSANT CONSTITUENT OF MURRAYA
KOENIGII L. LEAVES
7. PCOG-7 JITENDER SINGH COMPARATIVE ANTIANXIETY EVALUATION OF ARGYREIA SPECIOSA LINN.
(ROOTS), CAESALPINIA DIGYNA ROTTLER (ROOTS) AND SPHAERANTHUS INDICUS
LINN. (FLOWERS)
8. PCOG-8 MEENU BHAN IONIC LIQUIDS BASED MICROWAVE ASSISTED EXTRACTION- THE FUTURE OF
HERBAL EXTRACTION
9. PCOG-9 PREETI BANSAL MEDICINAL POTENTIAL OF THUJA ORIENTALIS (LINN.) FRANCHO
10. PCOG-10 UZMA AZEEM A MEDICINAL MUSHROOM PHELLINUS AN OVERVIEW
11. PCOG-11 DEEPAK KUMAR MALTOL: THE ANALGESIC CONSTITUENT OF ABIES PINDROW ROYLE
12. PCOG-12 HASANDEEP SINGH PHARMACOGNOSTIC INVESTIGATIONS AND PHARMACOLOGICAL EVALUATION OF
ALSTONIA SCHOLARIS R. BR. LEAVES IN NEUROPATHIC PAIN.
13. PCOG-13 ISHTDEEP KAUR STABILITY STUDIES ON CENTELLA ASIATICA EXTRACTS
14. PCOG-14 KUSUM LATA FRUIT PEELS AS POTENTIAL ANTICANCER AGENTS
15. PCOG-15 LAVI RAJPUT PHARMACOGNOSTIC AND ANALGESIC ACTIVITY SCREENING STUDIES OF
ADVENTITIOUS ROOTS OF FICUS RELIGIOSA LINN.
16. PCOG-16 NITTYA K. DOGRA EVALUATION OF ANTIANXIETY ACTIVITY OF CALOTROPIS GIGANTEA L. ROOTS
17. PCOG-17 PRIYANKA MITTAL ANTIANXIETY ACTIVITY INVESTIGATIONS ON A. PINDROW ROYLE AERIAL PARTS
18. PCOG-18 RAVINDER KAUR COMPARATIVE ANTI-ANXIETY EVALUATION OF TWO OCIMUM SPECIES
19. PCOG-19 SHIVANI THAKUR BIOACTIVITY DIRECTED ISOLATION OF ANTIANXIETY CONSTITUENT OF PINUS
ROXBURGHII SARG.
20. PCOG-20 HARPREET KAUR EVALUATION OF ANTI-ANXIETY AND ANTI-EPILEPTIC ACTIVITIES OF GREWIA
ASIATICA LEAVES
21. PCOG-21 SHANTI DEVI NATURAL IMMUNE MODULATORS
22. PCOG-22 NITIN VERMA EFFECT OF ALCOHOLIC EXTRACT OF ADIANTUM CAPILLUS-VENERIS ON COLD
IMMOBILIZATION STRESS INDUCED LIPID PEROXIDATION IN RATS
23. PCOG-24 DIKSHA SHARMA ANTIOXIDANT ACTIVITY OF HYDROETHANOLIC EXTRACT OF FRUIT PEEL OF
CITRUS LIMETTA VAR.MITHA
24. PCOG-25 AKASH JAIN HYPOLIPIDEMIC, HYPOGLYCEMIC AND ANTIOXIDANT POTENTIAL OF JASMINUM
GRANDIFLORUM ETHANOLIC LEAVES EXTRACT IN STREPTOZOTOCIN INDUCED-
EXPERIMENTAL DIABETES
25. PCOG-26 KUSUM KAUSHIK SUPERCRITICAL FLUID EXTRACTION
26. PCOG-27 MANMOHAN
CHAUHAN
VALUE OF DIGITALIS IN HEART FAILURE
27. PCOG-28 SHIVANI DHIMAN MICROWAVE ASSISTED EXTRACTION A NOVEL PROMISING TECHNIQUE FOR
EXTRACTION OF MEDICINAL PLANTS
28. PCOG-29 NAVNEET SHARMA A SYSTEMATIC REVIEW OF THE DRUG :-PANAX GINSENG
29. PCOG-30 SUPRIYA VERMA INCORPORATING HERBAL MEDICINE INTO CLINICAL PRACTICE
30. PCOG-31 SHALINI KANWAR MICROWAVE ASSISTED EXTRACTION
31. PCOG-32 SUNIL SAHARAN VOLATILE OILS; A PRESICIOUS GIFT OF NATURE
32. PCOG-33 VANDANA
VALECHA
CRATAEVA NURVALA: A VALUABLE MEDICINAL PLANT
33. PCOG-34 CHETAN SHARMA EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF GLYCYRRHIZA
GLABRA LINN. ROOTS EXTRACTS
34. PCOG-35 KUNWAR ANTIDIABETIC HERBAL FORMULATIONS AVAILABLE IN MARKET
35. PCOG-36 RAJINDER MANN PHARMACOGNOSTICAL AND PHYSICO-CHEMICAL EVALUATION OF THE LEAVES
OF CRYPTOLEPIS DUBIA (BURM.F.) M.R. ALMEDIA
36. PCOG-37 PRERNA SARUP CHEMOMETRIC ANALYSIS: A NOVEL TOOL FOR HERBAL DRUGS ANALYSIS
37. PCOG-38 SHASHIKANT
BHARDWAJ
HERBAL MEDICINES FOR THE MANAGEMENT OF DIABETES
38. PCOG-39 DEEPAK
DHANKHAR
HERBAL COSMETICS: AN OVERVIEW
39. PCOG-40 PRAVEEN KUMAR
GOYAL
DEVELOPMENT OF ANTI-STRESS MONO HERBAL FORMULATION OF WITHANIA
SOMNIFERA AND EVALUATE THE EFFECT OF EXCIPIENTS ON ITS DISSOLUTION
PROFILE
40. PCOG-41 ROMIKA EVALUATION OF ANTIMICROBIAL ACTIVITY OF CORIANDRUM SATIVUM FRUIT
EXTRACTS AGAINST MICROBES ASSOCIATED WITH FRUIT JUICE
41. PCOG-42 VIKAS KUMAR LEAF-SPOT DISEASE OF TRIANTHEMA PORTULACASTRUM A NEW RECORD FROM
WORLD
42. PCOG-43 M. KAUR ALTERNARIAMACROSPORA: A POTENTIAL FUNGAL PATHOGEN FOR BIOCONTROLOF
PARTHENIUMWEED
43. PCOG-44 PRIYA YADAV HERBS & DRUG INTERACTION
44. PCOG-45 SUNIL KUMAR APPLICATION OF NOVEL ULTRASOUND ASSISTED EXTRACTION TECHNIQUE FOR
THE HERBS
45. PCOG-46 PRAVEEN KUMAR
GOYAL
DEVELOPMENT OF ANTI-STRESS MONO HERBAL FORMULATION OF
WITHANIASOMNIFERA AND EVALUATE THE EFFECT OF EXCIPIENTS ON ITS
DISSOLUTION PROFILE
46. PCOG-47 PUSHPENDER MICROSCOPIC STUDY AND PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF
CHENOPODIUM ALBUM
47. PCOG-48 SHAKTI GOEL MOUTH ULCER PROTECTION BY JASMINUM GRANDIFLORUM
48. PCOG-49 GEETA DESWAL A REVIEW- TEAK
MISCELLANEOUS
S.NO PAPER
CODE
AUTHOR TITLE
1. MIS-1 MANU ARORA STANDARDIZATION OF MISWAK: AN ORAL HYGIENE TOOL
2. MIS-3 UMANSHU
DHINGRA
ANTIMICROBIAL RESISTANCE - A GLOBAL CONCERN. NO ACTION TODAY, NO
CURE TOMORROW
3. MIS-4 MANISHA PARAGLIDING THROUGH SWOT ANALYSIS ON USA'S CURRENT REGULATORY
ENVIRONMENT
4. MIS-5 PANKAJKUMAR AMENDMENTS IN SCHEDULED H DRUGS: CRITICAL ASSESSMENT
5. MIS-7 DIMPLE REGULATION OF MEDICAL DEVICES IN INDIA
6. MIS-8 PRERNA KAUSHIK INDIA- GRAPPLING WITH CONTROL OF DRUG PRICES
7. MIS-9 KAUSHIK VICHITRA INNOVATIONS FROM NATURE A NEW COMMENCE
8. MIS-10 AMANDEEP KAUR DOCTOR OF PHARMACY EDUCATION IN INDIA- A CRITICAL STUDY
9. MIS-11 DEEPA BATRA BIOMARKERS: AN EMERGING TOOL IN MEDICAL PRACTICE
10. MIS-12 MONIKA PUNIA QUALITY BY DESIGN
11. MIS-13 MOHIT MADAN EBOLA VIRUS : A DEADLY MENACE
12. MIS-15 GARIMA PARAMOUNT PART OF LIFE: SLEEP
13. MIS-16 KARISHMA PHARMACOVIGILANCE: A WAY FOR BETTER TOMARROW
14. MIS-17 SWEETY HOODA

WHAT, WHEN AND HOW OF THE PROMOTION OF RATIONAL USE OF MEDICINE
15. MIS-18 ANJALI GOYAL AVAILABILITY AND RELATIVE PRICE PAID FOR BRANDED MEDICINES FOR
COMMON AILMENTS IN TEN AREAS (100 PHARMACY OUTLETS) OF SONEPAT
DISTRICT
16. MIS-19 VIPIN SAINI DRUG SAFETY RISK MANAGEMENT PLAN
17. MIS-20 GARIMA KUMARI RISK OF SELF MEDICATION
18. MIS-21 ADITYA DELU PHARMACOGENOMICS- A NEED OF THE HOUR
19. MIS-22 ANJALI ANTIDOTE FOR OPIUM POISONING
20. MIS-23 GAGANPREET
KAUR
EVOLUTION OF PHARMACY PRACTICE
21. MIS-24 BHUMIKA GUPTA NOSOCOMIAL DISEASES OR HOSPITAL ACQUIRED DISEASES
22. MIS-25 VANDANA

SPECIFIC ABSORPTION RATE VARIATION IN BRAIN PHANTOM DUE TO EXPOSURE BY A 3G
MOBILE PHONE

1st Annual conference of APTI Haryana State Branch
August 22, 2014

Indian Scenario of Pharmaceutical education: Challenges and Future perspectives

PHARMACEUCICS
ABSTRACTS
August 22, 2014


PCEU-2

SOLID DISPERSION FAST DISSOLVING TABLETS OF GLIMEPIRIDE

Paaras Gupta, Ankita Kapoor, Raj kumara

School of Pharmacy and Emerging Sciences, Baddi University of Emerging Sciences and
Technology, Makhnumajra Baddi, Distt. Solan, H.P. -173205, India
E-mail: paarasgupta160@gmail.com

INTRODUCTION

A drug with poor aqueous solubility exhibit dissolution rate limited absorption, and solid
dispersion technologies are particularly promising for improving the oral absorption and
bioavailability of Biopharmaceutical Classification System Class II drugs.
METHODOLOGY

Solid dispersions of glimepiride in Polyethylene Glycol (PEG) 4000 and 6000 were prepared by
fusion method in ratios of 1: 1,1: 3,1: 5,1: 7. The polymer was melted in china dish. Drug was
added to the molten polymer with continuous stirring. Solid dispersion was cooled in a ice bath,
dried, sieved through 100# and stored in a dessicator. Solid dispersions having ratio 1:5 were
selected for formulation of fast dissolving tablets.
RESULTS AND DISCUSSION
Tablets were directly compressed, found to be elegant, white, odorless and smooth in appearance. The
tablets were 6 mm in diameter and spherical concave with thickness 2.39 0.06 mm to 2.95 0.01 mm,
weight variation in the range 88.25 1.51mg to 95.32 2.48 mg, hardness 2.4 0.26 Kg/cm
2
to 2.6
0.32 Kg/cm
2
,Friability 0.22 0.10 to 0.29 0.01 % less than 1% so tablets passes the friability test,
Wetting time 40.2 1.0 seconds to 50.5 0.1 seconds, Disintegration time 43.9 1.5sec to 50.9 1.5sec
and Drug Content 97.98 2.1 % to 99.23 1.6 %.
CONCLUSION
It is evident that the fast dissolving tablets containing solid dispersions exhibited a faster
dissolution when compared to fast dissolving tablets of pure drug.

PCEU-3

PHOSPHOLIPID AND MACROGOL BASED MIXED NANOMICELLES ENHANCE
ANTIOXIDANT STATUS OF MELOXICAM

Dhanila Varkey, Jessy Shaji, Ipshita Menon*

Department of Pharmaceutics, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe
Parade, Colaba, Mumbai-400005

Abstract:
Oxidative stress and decreased antioxidant status are the hallmarks in patients suffering from
Rheumatoid Arthritis (RA). A novel targeted nanomedicine can augment the therapeutic efficacy of
August 22, 2014


Meloxicam (MLX), a preferential COX-2 inhibitor with significant suppressive effects on free
radical mediated damage. In view of this, MLX-loaded mixed nanomicelles were fabricated by co-
precipitation reconstitution method using drug, lipid and macrogol-15 hydroxystearate. The
nanomicelles were characterized for particle size distribution and zeta potential using nanoparticle
tracking analysis. The mean particle size and zeta potential was found to be 88 42 nm and -47.4
16.2 mV. Transmission electron microscopy and atomic force microscopy studies confirmed the
production of nanomicelles. HPLC results demonstrated high encapsulation efficiency over 90%. In
vitro cytotoxicity of MLX in nanomicelles was determined on the RAW 264.7 cell line. The in vitro
antioxidant potential of nanomicelles was evaluated by various antiradical and antioxidant assays
including DPPH free radical scavenging, nitric oxide radical inhibition, lipid peroxidation, hydroxyl
radical scavenging and superoxide anion radical scavenging activity. Free radical scavenging activity
of MLX loaded mixed nanomicelles in all assays was higher than the free drug and was found to
increase in a dose-dependent manner. A nanomicelles based delivery system for MLX potentiates its
free radical suppression effects and can further enhance its therapeutic efficacy in the treatment of
RA.

PCEU-4

HYDROPHOBIC DRUG KETOPROFEN IN TOPICAL EMULGEL

Ankita Kapoor, Richa Sharma, Manu Arora, Ramit Kapoor
School of Pharmacy and Emerging Sciences, Baddi University of Emerging Sciences and
Technology, Makhnumajra Baddi, Distt. Solan, H.P. -173205, India
E-mail: ankita8620@yahoo.com

BACKGROUND
When gel and emulsion are used in combined form they are referred as emulgel. Emulsion in gel
have emerged as one of the most interesting topical drug delivery system as it have dual release
control system i.e. emulsion and gel

METHODS
The gel bases were prepared by dispersing Carbopol 934 and HPMC K4M in distilled water with
continuous stirring using a mechanical shaker. The pH of formulations was adjusted using
Triethanolamine. The oil phase of emulsion was prepared by dissolving Span 80 in Light Liquid
Paraffin while the aqueous phase was prepared by dissolving Tween 80 in purified water. Methyl
Paraben was dissolved in Propylene Glycol and mixed with aqueous phase. Acetone was mixed
in aqueous phase. Ketoprofen was dissolved in oil phase. Oleic acid was also mixed in oil phase.
Both the oil and aqueous phase were then separately heated to 70-80C then the oily phase was
added to aqueous phase with continuous stirring until it get cooled to room temperature.
EVALUATION
It was evaluated physically, pH was determined, viscosity was estimated, spreading coefficient
was determined and extrudability test was performed, drug content and in vitro release studies
were performed. It was compared with the emulgel present in market i.e., Voltaren
.
emulgel of
Diclofenac.
CONCLUSION
August 22, 2014


Ketoprofen belongs to class of Non-steroidal Anti-inflammatory drugs. This drug when applied
topically used to treat swelling, sprains, backache or arthritis. Oral drug cause gastric irritation or
bleeding so to overcome this Ketoprofen emulgel can be promising formulation.

PCEU-5

FORMULATION AND EVALUATION OF NANOSIZED ETHOSOMES FOR
ENHANCED TRANSDERMAL DELIVERY OF KETOROLAC TROMETHAMINE

Akshay Shah
*
, Sharvari Garude, Jessy Shaji
Department of Pharmaceutics Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe
Parade, Mumbai 40005. India

Abstract
The aim of this investigation is to develop nanosized ethosomes for improved transdermal
permeation of ketorolac tromethamine. Ethosomes are soft, elastic vesicle composed of
phospholipids and high concentration of ethanol. Ethosomes was prepared by cold method and
was evaluated for various parameters like vesicle size, zeta potential, % drug entrapment, ex-vivo
permeation, in-vivo study. Microscopic examination suggested ethosomes as spherical
unilamellar vesicles with a smooth surface. Optimized ethosomal vesicles were of size
13460nm with zeta potential of -42.412.4mv and drug entrapment was 79.833.18%. Ex-vivo
permeation studies across porcine skin resulted in increased flux of 40.380.24g/cm
2
/hr when
compared with hydroethanolic drug solution (17.330.05g/cm
2
/hr) and plain drug solution
(15.050.84g/cm
2
/hr). The enhancement ratio of ethosomes was found to be 2.7 fold higher to
plain drug solution and 2.4 fold higher to hydroethanolic solution. Data obtained from this
experimental work concludes that ethosomal formulation is safe and very efficient as a drug
carrier for accentuated transdermal delivery of ketorolac tromethamine which could serves as a
potential carrier system.

PCEU-6

OPTIMIZATION AND CHARACTERIZATION OF NOVEL ALGINATE-CHITOSAN
NANOPARTICLES CONTAINING PAYLOAD OF ANTI TUBERCULAR DRUG
CYCLOSERINE.

Mahmood Shaikh
*
, Jessy Shaji
Department of Pharmaceutics, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe
Parade, Mumbai 40005, Maharashtra, India

Abstract
Management of Tuberculosis is mainly affected by the drug resistance due to the poor patient
compliance, this can be overcome by reduction in the dosing frequency of antitubercular drugs
August 22, 2014


by applying drug delivery technology which has the potential to improve the patient compliance.
Thus, in the present study, Cycloserine loaded Alginate-Chitosan nanoparticles were prepared by
a modified ionotropic gelation method and the particle characteristics including morphology and
particle size measurements were performed by Scanning electron microscopy, Transmission
electron microscopy and Particle size analyzer. Polymer interaction and drug incorporation was
confirmed by FTIR and DSC. Drug content, encapsulation efficiency and particle properties such
as size, polydispersity index and zeta potential were also determined. The particle and its release
characteristics can be efficiently optimized using the 2
3
factorial designs of experiments by
Design Expert V9. Response surface plots were drawn, statistical validity of the polynomials was
established and optimized formulations were selected by feasibility and grid search. The average
particle size ranged between 176 nm to 441 nm respectively. Drug entrapment ranged 97.58% to
99.21% respectively. The in vitro release studies show initial burst release followed by
controlled and uniform release for longer duration. The results suggested that ionotropic
controlled gelation can lead to the preparation of particles with favorable size, high entrapment
efficiency and controlled release characteristics serving as a convenient delivery system for
Cycloserine which has the potential for intermittent therapy of Multi-drug resistant Tuberculosis.

PCEU-7

MICROBIALLY SYNTHESIZED HIGH-QUALITY NANOSTRUCTURES FOR DRUG
DELIVERY SYSTEMS: A GREEN SYNTHESIS APPROACH

Sushil Nagar*, Anita Dua
Department of Biochemistry, University College, Kurukshetra University, Kurukshetra
Email: sushil661@gmail.com

Abstract
Nanotechnology is one of the fast budding sciences over the last recent years. The discipline of
nanotechnology connects life science, physical sciences and chemical science to each other with
the help of engineering. Particulate systems like nanostructures/nanoparticles have been used as
a physical approach to alter and improve the pharmacokinetic and pharmacodynamic properties
of various types of drug molecules. Nanoparticles can be geared up from a diversity of resources
such as proteins, polysaccharides and synthetic polymers. The selection of matrix materials is
dependent on many factors such as size of nanoparticles required; surface characteristics;
biodegradability and antigenicity of the final product. The production of nanoparticles with
irradiation, photochemical reduction technology or other techniques remains expensive and
involves perilous chemicals. Therefore, there is a growing concern to develop eco-friendly and
sustainable methods for production of nanoparticles. Microbial synthesis of nanoparticles is a
green chemistry and cost-effective approach. Biosynthesis of many metals such as Au, Ag, Se,
Te, Pt, Si etc nanoparticles by microorganisms have been reported. However, despite the stability
and delivery of drugs the nanoparticles are not mono-dispersed and their production is also slow.
Secondly, Inhibition of the production by the generation of free radicals from some metals is also
a critical problem for scientists. Nanoparticles mainly minimize drug degradation time upon
administration; prevent undesirable side effects, also increase drug bioavailability and a fraction
August 22, 2014


of the drug accumulated in the pathological area. Remaining to the rich biodiversity of microbes,
their potential as biological materials for nanoparticles synthesis is yet to be fully explored.

PCEU-10

LONG CIRCULATING SURFACE DECORATED LIPOSOMES FOR EFFICIENT
TREATMENT OF MDR CANCER

Sushama Talegaonkar* and Lalit M. Negi
Dept of Pharmaceutics, Faculty of Pharmacy, jamia Hamdard, new Delhi-62, India
Email: stalegaonkar@gmail.com

Abstract
Imatinib mesylate is a proclaimed tyrosin kinase inhibitor with indication in Chronic
myelogenous leukemia CML and Gastrointestinal stomal tumor GIST. However, due to
significant reporting of P-gp expression in cancer, it can come across set back due to MDR.
Therefore, in present work the liposomal formulation containing imatinib-bile salt conjugate was
developed and investigated for its comparative performance in MDR colon cancer cells.
Furthermore, the liposomes were surface modified with hyaluronic acid for achieving low
hemotoxicity with stealth characteristics. Imatinib mesylate was successfully conjugated with
sodium deoxycholate by charged conjugation and evaluated through FTIR, DSC and PXRD. The
developed conjugate (IM-SD) was encapsulated in liposomes and the conditions were optimized
by Box-Behnken statistical design to achieve a size of 56.56 1.23 nm along with 99.11 0.89
% entrapment efficiency (LIPO). The liposomes were surface modified with hyaluronic acid and
the size was enhanced to 159.14 3.2 nm (HA-LIPO). Flow cytometric studies demonstrated the
enhanced uptake of P-gp substrate rhodamine dye in P-gp positive colo 320 colon cancer cells. In
addition, an enhanced cellular internalization of HA-LIPO in CD-44 positive HT-29 and colo
320 cells indicates the targeting attributes of the hyaluronan coated liposomes. Finally, the
hyaluronan coated liposomes were also found to have low blood interaction, low opsonization
and reduced hematological side effects.

PCEU-11

Effect of Polymers on Solid Dispersions of Aceclofenac

Priyanka Verma
Department of Pharmaceutical Sciences, G. J. University of Sci. & Tech., Hisar-125001, India.

Abstract
Aceclofenac is a non-steroidal anti-inflammatory drug (NSAID) having anti-inflammatory and
analgesic properties, and is widely used in the treatment of rheumatoid arthritis, osteoarthritis,
and ankylosing spondylitis. One of the major problems with this drug is its low solubility in
August 22, 2014


biological fluids, which results in poor bioavailability after oral administration. Solid dispersion
is one of the approaches that significantly enhance dissolution of poorly water-soluble drugs. The
aim of present study was to enhance the solubility of poorly water-soluble drug aceclofenac by
solid dispersion techniques using polyethylene glycols (PEG 8000) and polyvinyl pyrrolidone
(PVP K-30) as carriers in ratios 1:5, 1:10, 1:15. Solid dispersions (a dispersion of one or more
active ingredients in an inert carrier or matrix at solid state) were prepared by physical mixture,
fusion method and solvent evaporation method. The prepared solid dispersions were evaluated
by Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction. In vitro
dissolution studies were carried out in phosphate buffer (pH 6.8) and analyzed
spectrophotometrically at the
max
of 274 nm.Faster dissolution was exhibited by solid
dispersions containing 1:15 ratio of drug: PEG 8000 by fusion method. The increase in
dissolution rate of the drug could be attributed to increase in wettability, hydrophilic nature of
the carrier and due to reduction in drug crystallinity.The results of solid state characterization
indicated that in solid dispersions the crystalline drug gets converted to its amorphous form.
FTIR study results indicated the absence of interaction between aceclofenac and carriers.In X-
ray powder diffraction less intensity of drugpeaks showed that the drug has acquired the
amorphous characteristics.

PCEU-13

NATURAL POLYMERS BASEDMAGNETIC MICROSPHERES AS A EFFECTIVE
TOOL FOR CHRONIC DISEASE

Lokesh Sadana
*, 1
, Neha Khar
1
, Surender Verma
1
1
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra
E-mail:-sadana.lokesh@gmail.com

Abstract
Microspheres are free flowing powders consisting of encapsulated (drugs) spherical particles of
size ideally less than 125p that can be suspended in aqueous vehicle.Magnetic microspheres hold
great promise for reaching the goal of controlled and site specific drug delivery. Magnetic
microspheres are traditional radiation methods which uses highly penetrating radiations thatare
absorbed throughout the body.Various Natural polymers are used like albumin, chitosan, gelatin,
starch, polystarch, agarose etc. Now a days targeted treatment system are used in many chronic
diseases such as cancer, diabetes, nerve damage etc.Chronic Disease is a long
lasting condition that can be controlled but not cured. Therefore, the in-vivotargeting of tumors
with magnetic microspheres is currently realized through the application of external non-uniform
magnetic fields generated by rare-earth permanent magnets or electromagnets. Magnetically
controlled drug targeting is one of the various possible ways of drug targeting.There has been
keen interest in the development of a magnetically target drug delivery system. These drug
delivery systems aims to deliver the drug at a rate directed by the needs of the body during the
period of treatment, and target the activity entity to the site of action. Its use is limited by toxicity
and side effects. The aim of the specific targeting is to enhance the efficiency of drug delivery &
August 22, 2014


at the same time to reduce the toxicity & side effects. This paper gives an overview of the
mechanism, benefits, drawbacks, preparation and applications of magnetic microspheres.

PCEU-14

CARBON NANOTUBES AS A DRUG DELIEVERY SYSTEM: A REVIEW

Rajni Bala*, Neha kanojia

Chitkara College of Pharmacy, Chandigarh - Patiala National Highway, Rajpura
140401,Patiala, Punjab, India.

Abstract
Drug delivery is a field of research and is gaining the attention of pharmaceutical researchers,
medical doctors and industry. A safe and targeted drug delivery could improve the performance
of some drugs already on the market, and will have implications for the development and success
of new therapeutic approach for the delivery of anticancer, peptide and protein drugs and gene
therapy. In the last decade, several drug-delivery technologies have emerged and an interesting
part of this field is the development of nanoscale drug delivery system. Nano particles (NPs)
have been developed as an important approach to deliver conventional drugs, recombinant
proteins, vaccines and more recently, nucleotides. NPs and other colloidal drug-delivery systems
modify the kinetics, body distribution and drug release of associated drug molecule by
improvement of atomic and molecular properties of drug. This review focuses on the potential of
nanotechnology in medicine particularly carbon nanotubes as well as their applications in
therapeutics, diagnostics and imaging and their future prospects.

PCEU-15

FABRICATION AND EVALUATION OF ORO-DISPERSIBLE PECTIN-HPMCE15LV
FILMS OF PROPANOLOL HYDROCHLORIDE

Bhupinder Kaur

, Newton AMJ, Rajesh Kumar, Manoj Kumar Katual
Department of Pharmaceutics, Rayat-Bahra Institute of Pharmacy, Education City, Hoshiarpur,
India (146001).
Email: monasaini918@gmail.com

ABSTRACT
The prime aim of this research work was to develop the oro-dispersible films of Propanolol HCl.
Oro-dispersible films are gaining popularity over other dosage forms in the current market. A
non cardio selective - adrenergic antagonist Propanolol HCl was used as a model drug. The half
life of Propanolol HCl is 4h and is soluble in water with the bioavailability of 42%. The rationale
behind using Propanolol HCl is that it is used in the treatment of hypertension and hypertensive
August 22, 2014


emergencies where a quick onset of action is inevitable. The formulations were studied for
preformulation characteristics and compatibility study before developing the dosage form. The
drug excipient compatibility studies were carried out by using FTIR and DSC and the results
ruled out any interaction between drug and other excipients. The oro-dispersible films were
prepared by using HPMCE15LV premium grade polymer and HPMC15cps. The HPMCE15LV
is the premium grade polymer which is superior to regular HPMC15cps and possessing excellent
film forming characteristics. Different techniques were carried out the in trials and the solvent
casting methods were used in the development of oro-dispersible units. Since the drug
Propanolol HCl is bitter in taste, the sweeteners and salivary stimulating agent citric acid were
incorporated in the formulation in order to facilitate the film solubility in the oral cavity. The
films were evaluated for the various essential quality control parameters such as tensile strength,
texture analysis, drug content, dissolution and disintegration studies. The films were also
evaluated against the commercially available brands.

PCEU-16

CHRONOTHERAPEUTIC DRUG DELIVERY OF PECTIN, GUAR GUM AND OKRA
GUM CONTROLLED RELEASE COLON TARGETED DIRECTLY COMPRESSED
PROPRANOLOL HCL MATRIX TABLETS AND I N-VI TRO EVALUATION

Jatinder Kumar, Newton AMJ, Rajesh Kumar, Manoj Kumar Katual
Department of Pharmaceutics, Rayat- Bahra Institute of Pharmacy Education City, Hoshiarpur,
India (146001).
Email: jatinderkumar918@gmail.com.

ABSTRACT
The prime aim of this investigation is to develop a delayed release formulation with various
natural polysaccharides such as Pectin, Okra gum and Guar gum. A model drug Propranolol HCl
was used in this formulation to target the drug candidate to the colon. Assessment of colon
targeting and controlled release potential of the developed novel dosage form was the main
concept of this research. The naturally available polysaccharides such as Pectin and Okra gum
were used as prime polymers in this study to compare the superiority of the polymer over each
other.Plenty of research available on conventional Guar gum tablets, was reported for
manufacturing and bioavailability problems in large scale production. To overcome these
drawbacks of Guar gum the study has been designed. The matrix tablets of Propranolol HCl were
prepared by direct compression. The various recommended parameters of the tablets were
evaluated and the results were found to be within the limits. The carbopol 940 was used as an
auxiliary polymer to modify the drug release and compression characteristics of the tablets. The
in vitro release studies were carried out first in 0.1N HCl for 1h and 30 minutes and in pH 6.8
phosphate buffer for 2h and after that pH 7.4 phosphate buffer until the maximum amount of
drug is released. The release kinetics of the formulations were analyzed by various famous
pharmacokinetic models such as First order, Zero Order, Higuchi, Hixon-Crowell and
Korsmeyer-Peppas model and mechanism of drug release was examined and reported.

August 22, 2014


PCEU-17

FORMULATION AND EVALUATION OF ORO-DISPERSIBLE TABLETS FOR ANTI-
DEPRESSENT DRUG CITALOPRAM HYDROBROMIDE.

Mandeep Singh
*
, Newton AMJ, Manoj Kumar Katual ,Rajesh Kumar Sachdeva
India (146001).
Email: mandeepsinghrbip@gmail.com

ABSTRACT
The aim of present study was Formulation development and Evaluation of Oral fast dispersible
tablets of Citalopram hydrobromide. The formulations were studied for pre-formulation and
drug-excipient compatibility study in FTIR before the development of formulation. In the study,
all of the powder blend showed good flow properties. The bulk density, tapped density and the
compressibility index were found within the range which ensured the blend was suitable for
direct compression into tablets. Oro-dispersible tablets of Citalopram were prepared by Direct
Compression method with Mannitol, Micro crystalline cellulose alone and both in combination
as diluents. The super disintegrants such as Sodium starch glycolate and povidone with cross
carmellose were used in formulation. The prepared tablets were subjected to various parameters
like uniformity of weight, hardness, friability, drug content, water absorption ratio, wetting time,
in vitro disintegration time and in vitro dissolution studies. The effect of different super
disintegrants over the drug release profile was investigated. In vitro disintegration time for all
batches i.e. CF-1 to CF-6 showed wide variation in the range between 17.64 to 27 seconds and %
Drug dissolved at 60 seconds (DP
60
) for all formulation batches i.e. CF-1 to CF-6 showed wide
variation in the range between 18.10 to 96.45% which was found to be the better results. The
prepared formulations containing super disintegrants sodium starch glycolate was found to be
better and optimized formulation according to rate of dispersion and dissolution profile.

PCEU-18

FORMULATION AND EVALUATION OF ORO-DISPERSIBLE TABLETS FOR ANTI-
DEPRESSENT DRUG CITALOPRAM HYDROBROMIDE.

Mandeep Singh
*
, Newton AMJ, Manoj Kumar Katual ,Rajesh Kumar Sachdeva
India (146001).
Email: mandeepsinghrbip@gmail.com

ABSTRACT
The aim of present study was Formulation development and Evaluation of Oral fast dispersible
tablets of Citalopram hydrobromide. The formulations were studied for pre-formulation and
drug-excipient compatibility study in FTIR before the development of formulation. In the study,
August 22, 2014


all of the powder blend showed good flow properties. The bulk density, tapped density and the
compressibility index were found within the range which ensured the blend was suitable for
direct compression into tablets. Oro-dispersible tablets of Citalopram were prepared by Direct
Compression method with Mannitol, Micro crystalline cellulose alone and both in combination
as diluents. The super disintegrants such as Sodium starch glycolate and povidone with cross
carmellose were used in formulation. The prepared tablets were subjected to various parameters
like uniformity of weight, hardness, friability, drug content, water absorption ratio, wetting time,
in vitro disintegration time and in vitro dissolution studies. The effect of different super
disintegrants over the drug release profile was investigated. In vitro disintegration time for all
batches i.e. CF-1 to CF-6 showed wide variation in the range between 17.64 to 27 seconds and %
Drug dissolved at 60 seconds (DP
60
) for all formulation batches i.e. CF-1 to CF-6 showed wide
variation in the range between 18.10 to 96.45% which was found to be the better results. The
prepared formulations containing super disintegrants sodium starch glycolate was found to be
better and optimized formulation according to rate of dispersion and dissolution profile.

PCEU-19

A STUDY ON HPMC K4M AND CARBOPOL 940 BASED AMOXICILLIN
TRIHYDRATE FLOATING TABLETS AND I N VI TRO EVALUATION FOR THE
TREATMENT OF H.PYLORI INFECTION.

Sandeep Kaur , Newton AMJ, Manoj Kumar Katual, Rajesh Kumar

Department of Pharmaceutics, Rayat Bahra Institute of Pharmacy, Education City, Hoshiarpur,
Punjab, India.146001
Email: sandeepbains2711@gmail.com

Abstract
The prime aim of this research work was to develop a floating drug delivery system of
Amoxicillin trihydrate. The HPMC K4M and Carbopol 940 was used as a prime polymer in
developing a floating dosage form. The HPMC K4M was used as hydrophilic polymer to form a
matrix and control the drug release, whereas Carbopol 940 also influenced the controlled release
profile and offers the swelling property by which it facilitates floating. The main reason for
developing Amoxicillin trihydrate as a floating drug delivery system was to treat H.pylori
infections in the stomach and to provide localised drug concentration. The excipients such as
sodium bicarbonate, citric acid were incorporated in the formulation as a gas forming agent. The
tablets were evaluated for floating profile, drug release profile and swelling property. The other
essential quality control parameters of tablets such as weight variation, friability, drug content
uniformity also evaluated and reported. Previously the preformulation characteristics such as
flow property, packing property and drug excipient compatibility study in FTIR and DSC was
also studied before developing the dosage form. The in vitro studies were performed in 0.1N HCl
for 12 hours. The mechanism of drug release and release kinetics were evaluated by using
suitable pharmacokinetic models such as Zero order, First order and Higuchi kinetics.

August 22, 2014


PCEU-20

IMPROVEMENT OF DISSOLUTION PROFILES OF WATER INSOLUBLE DRUG
ITRACONAZOLE USING CRYSTALLINE AND AMORPHOUS CARRIER

Tejpal Singh

, Ashish K Garg, Rajesh Kumar, Newton AMJ, Manoj Kumar Katual
Department of Pharmaceutics, Rayat-Bahra Institute of Pharmacy,
Education City, Hoshiarpur, Punjab, India (146001).
Email: sainitejpal46@gmail.com

ABSTRACT
The present study compares the effect of crystalline (Poloxamer-407) and amorphous carrier
(modified Gum Karaya) for improvement of dissolution profile of broad spectrum antifungal
agent Itraconazole (BCS Class II). Using solvent evaporation method, solid dispersion of
Itraconazole was prepared with modified gum karaya, which released up to 86% of drug at the
end of 2 hours but it possess low drug content and low drug yield. Similarly, solid dispersion of
drug was prepared with poloxamer-407 using hot melt method, which released 92% of drug at
the end of 2 hours with good percentage yield and drug content. Poloxamer-407 provided
complete amorphous state and absence of crystalline peaks while modified Gum Karaya showed
small number of crystalline peaks with much reduced intensity shifting towards lower melting
endotherm.

PCEU-21

SOLID SMEDDS AS POTENTIAL CARRIER FOR
NOVEL DRUG DELIVERY SYSTEMS

Garima Singh*, KanavMidha, ManjuNagpal and Sandeep Arora
Chitkara College of Pharmacy, Chitkara University, Chandigarh- Patiala National Highway,
Rajpura- 140401, India
E-mail: singh.garima2490@gmail.com

Abstract
Recent developments in the area of excipients led to increased interest in development of lipid-
based formulations in last few years. Oral lipid-based formulations are composed of water-
soluble organic solvents (polyethylene glycol 300, propylene glycol ethanol), oily lipid vehicles
(medium chain mono and diglycerides or long-chain triglycerides), non-ionic surfactants and co-
surfactants (Tween 20, Span 80, Cremophore RH 40, Gelucires
Labrasol
and Labrafils
). Such
lipid based formulations are defined as isotropic mixtures of natural or synthetic oils, solid or
liquid surfactants or alternatively, one or more hydrophilic solvents and co-solvents/surfactants.
Upon mild agitation followed by dilution in aqueous media, these systems can form fine oil-in-
water (o/w) emulsions or self-microemulsifying drug delivery system (SMEDDS).These systems
present the drug in a solubilised form in GIT, and the small size of the formed droplet provide a
large interfacial surface area for drug absorption. SMEDDS are generally formulated either as
liquids or encapsulated in soft gelatin capsules which have some drawbacks especially in
August 22, 2014


manufacturing process (high production cost), incompatibility with the shells (brittleness or
softness of shell), limited dosage preparation and storage temperature. Thus liquid SMEDDS are
converted into solid SMEDDS by various techniques such as high pressure homogenization,
supercritical fluid based technology, melt extrusion, melt granulation, spray drying, spray
cooling and adsorption using solid carriers. The most viable technique used in lab scale is
adsorption using solid carriers. Various solid carriers have been successfully used for the
development of solid SMEDDS are Neusilin
, Fujicalin
, Aerosil
200, Aerosil
300, Sylsia,
Dextran, Maltodextran, Microcrystalline cellulose and Coffee husk. This review article gives an
insight about the ongoing research using these carriers; including a comparative evaluation of
these carriers for successful development into solid dosage forms.

PCEU-22

APPLICATIONS OF MICROWAVE TECHNOLOGY FOR POLYMER GRAFTING

Priya Rani *, Inderbir Singh and Sandeep Arora
Department of Pharmaceutics, Chitkara College of Pharmacy, Chitkara University, Rajpura-
140401, Patiala, Punjab
E mail: pnangru@gmail.com

Abstract
The use of microwave irradiation has become a common heat source in organic chemistry. The
microwave irradiation is also increasingly studied for polymerization reactions. Microwave
technique offer a number of advantages like rapid reactions, high purity of products, less side-
products, improved yields, simplified and improved synthetic procedure, wider usable range of
temperature, higher energy efficiency, sophisticated measurement and safety technology,
modular systems enable changing from mg to kg scale. However certain disadvantages like heat
force control is difficult, water evaporation and closed container is dangerous because it could be
burst, are also associated with this technique. The present review discusses the technique and
principle involved in microwave assisted technique for grafting of the polymers. Various
pharmaceutical applications like release retardant, tablet superdisintegrant, flocculant,
mucoadhesion of the microwave assisted grafted polymers have been discussed. Microwave
assisted extraction of various compounds from materials of natural origin is also gaining
importance among researchers. In conclusion, microwave assisted grafting of polymers for
specific application is a useful technique modifying important pharmaceutical properties of the
polymers.

August 22, 2014


PCEU-23

PREPARATION AND EVALUATION OF FLOATING TABLETS OF AN
ANTIHYPERTENSIVE DRUG

Gurpreet Kaur, Sonia Dhiman, Priyanka Yadav, Nischay Singla
Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India

Abstract
The present study was carried out with an objective of preparation and in vitro evaluation of
floating tablets of atenolol using hydroxypropyl methyl cellulose, ethyl cellulose, beeswax and
sodium bicarbonate in combination. The floating matrix tablets of atenolol were prepared by
melt granulation method. Beeswax was used as hydrophobic meltable material. Atenolol is an
antihypertensive drug with an oral bioavailability of only 50% because of its poor absorption
from lower gastrointestinal tract. The floating tablets of atenolol were prepared to increase the
gastric retention, to extend the drug release, and to improve the bioavailability of the drug. The
drug- polymer interaction was studied by Differential Scanning Calorimetry (DSC) and Fourier
transform infrared spectrophotometer (FT-IR). The formulations were evaluated for physical
parameters like thickness, hardness, friability, uniformity of weight, drug content, buoyancy time
and in vitro drug release. The formulations were optimized on the basis of buoyancy time and in
vitro drug release. The present study shows that polymers like HPMC, ethyl cellulose and
beeswax in combination with sodium bicarbonate as a gas generating agent can be used to
develop sustained release floating tablets of atenolol. And also the combination of hydrophobic
and hydrophilic polymer minimized the burst release of drug from the tablet and achieved a drug
release by zero-order kinetics, which is practically difficult with only hydrophilic polymer.

PCEU-24

FORMULATION AND EVALUATION OF GASTRORETENTIVE TABLETS USING
COMBINATION OF HYDROPHILIC AND HYDROPHOBIC POLYMERS

Priyanka Yadav, Sonia Dhiman, Gurpreet Kaur, Lakshya Jain
Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India

Abstract
The present study was undertaken with an aim to formulate, develop and evaluate gastroretentive
floating tablets of a Calcium channel blocker, Nefidipine which release the drug in a sustained
manner over a period of 18 h. Combination of hydrophobic retardant namely ethyl cellulose and
a hydrophilic polymer hydroxy propyl methyl cellulose (HPMC) K15M were used in different
combinations as per different ratios for the preparation of tablets. The tablets were evaluated for
tablet thickness, hardness, weight variation, friability, floating lag time and in vitro drug release.
The in vitro release study of the tablets was performed in 0.1 N HCl as a dissolution media.
Formulation F6 with hydrophilic polymer (HPMC K15M) and hydrophobic retardant (ethyl
cellulose) in the ratio 1:3 was considered as an optimized formulation. The optimized
August 22, 2014


formulation showed satisfactory sustained drug release and remained buoyant on the surface of
the medium for more than 18 h. It can be concluded that floating drug delivery system of
Nefidipine can be successfully formulated as an approach to increase gastric residence time and
thereby improving its bioavailability.

PCEU-25

DEVELOPMENT AND EVALUATION OF TIMOLOL MALEATE LOADED
CHITOSAN NANOPATICLES FOR OCULAR DELIVERY

Ajay Saroha, Deepak Kaushik*
*Department of pharmaceutical sciences M.D.University Rohtak-124001
e-mail- ajay.saroha18@gmail.com

Abstract
Timolol Maleate-loaded Chitosan(CS) nanoparticles were prepared by ionic gelation method.
Experimental variables such as molecular weight of CS and the amount of crosslinking agent
(Sodium tripolyphosphate) were varied to study their effect on drug entrapment efficiency,
particle size and release rate of nanoparticles by using Box-Behnken design. Chemical stability
of timolol maleate (TM) and crosslinking of CS were confirmed by Fourier transform infrared
spectroscopy. Differential Scanning Calorimetry studies were performed on drug-loaded
nanoparticles to investigate crystalline nature of drug after entrapment. Result indicated
amorphous dispersion of drug in the polymer matrix. Scanning Electron Microscopy revealed
regularly shaped particles and mean particle size of nanoparticles was found to be 190 to 1500
nm. In-vitro release studies were performed in phosphate buffer saline of pH 7.4. A slow release
of TM up to 7 h was observed.

PCEU-26

DESIGN AND EVALUATION OF EFFERVESCENT FLOATING TABLETS OF
CEFUROXIME AXETIL

Isha*, Deepak Kaushik
*Department of Pharmaceutical Sciences, M. D. University, Rohtak.

ABSTRACT
Effervescent Floating Tablets containing Cefuroxime Axetil (CA) as model drug and HPMC
K4M as hydrophilic polymer were prepared by direct compression technique. A Box-Behnken
statistical design with 3 factors, 3 levels, and 17 runs is selected for the optimization study.
Concentration of HPMC K4M, Sodium bicarbonate and talc were varied to study their effect on
percentage cumulative drug release. To study analytical profile of Cefuroxime axetil , FTIR and
DSC studies were carried out. In vitro dissolution studies were performed in 0.1N HCl and
release of CA upto 8h was observed. For evaluation of floating behavior of tablets, in vitro
August 22, 2014


buoyancy studies were carried out. Floating tablets from each batch show floating time for more
than 12 h. All formulations showed acceptable specifications for weight variation, thickness,
hardness and friability. By fitting the data into zero order, first order, Higuchi and Korsemeyer-
Peppas model, it concludes that the release followed Higuchi model.

PCEU-27

DESIGN AND EVALUATION OF EFFERVESCENT FLOATING TABLETS OF
CEFUROXIME AXETIL

Isha*, Deepak Kaushik
*Department of Pharmaceutical Sciences, M. D. University, Rohtak.

ABSTRACT
Effervescent Floating Tablets containing Cefuroxime Axetil (CA) as model drug and HPMC
K4M as hydrophilic polymer were prepared by direct compression technique. A Box-Behnken
statistical design with 3 factors, 3 levels, and 17 runs is selected for the optimization study.
Concentration of HPMC K4M, Sodium bicarbonate and talc were varied to study their effect on
percentage cumulative drug release. To study analytical profile of Cefuroxime axetil , FTIR and
DSC studies were carried out. In vitro dissolution studies were performed in 0.1N HCl and
release of CA upto 8h was observed. For evaluation of floating behavior of tablets, in vitro
buoyancy studies were carried out. Floating tablets from each batch show floating time for more
than 12 h. All formulations showed acceptable specifications for weight variation, thickness,
hardness and friability. By fitting the data into zero order, first order, Higuchi and Korsemeyer-
Peppas model, it concludes that the release followed Higuchi model.

PCEU-28

DESIGN AND DEVELOPMENT OF SILYMARIN NANOPARTICLES FOR
ENHANCED HEPATOPROTECTIVE ACTIVITY

Priti Girotra*, Swati Gupta, Shailendra Kumar Singh
Department of Pharmaceutical Sciences, G. J. University of Sci. & Tech., Hisar-125001, India.
email: ph.arora13@gmail.com

Abstract
Silymarin, a flavonoid obtained from the seeds of Silybum marianum L. (family Asteraceae),
possess good hepatoprotective activity. The present investigation was aimed at formulation
optimization of polymeric nanoparticles using chitosan, for enhancing the hepatoprotective
activity of silymarin through passive targeted delivery, thereby enhancing its therapeutic
potential. The chitosan nanoparticles containing silymarin were prepared by ionic gelation
technique, followed by optimization using central composite design in order to minimize the
August 22, 2014


particle size and maximize the drug entrapment efficiency. The optimized formulation was
characterized for particle size, entrapment efficiency, in-vitro dissolution studies and was also
subjected to in vivo study on Swiss Albino mice using CCL
4
induced hepatotoxicity as an
experimental model. In vitro dissolution studies demonstrated sustained, zero order drug release
from optimized formulation. In vivo studies on Swiss Albino mice using CCL
4
induced
hepatotoxicity model, suggested that the therapeutic efficacy of silymarin loaded nanoparticles
was greatly amplified. Thus, the nanoparticles of silymarin were successful in enhancing its
passive liver targeting for improved hepatoprotection.

PCEU-29

ACCELERATED STABILITY STUDY OF SHANKHPUSHPI AND BRAHMI SYRUP

Nancy* and Gulshan Bansal*
Department of Pharmaceutical Sciences and Drug Research, Punjabi University,
Patiala - 147002, India

Abstract
Due to complex chemical nature of herbal products, the process of establishment of quality and
safety of the product throughout its entire storage duration is a tedious job. Various guidelines on
establishing safety and efficacy of herbal products through stability studies have been laid down
by the agencies like World Health Organization (WHO), European Medicines Agency (EMEA)
and International Conference on Harmonization (ICH). However, minimal efforts have been
done to establish their stability profile. CNS active herbal medicinal products form a significant
portion of global herbal market. These are consumed by population of all age groups
indiscriminately without any prescription. Many such products manufactured by various drug
houses are available in the market. Hence, the present study is designed to conduct the stability
testing of commercially available herbal formulations used for CNS disorders under the
guidelines specified by WHO. An accelerated stability study is carried out on two commercially
available formulations of Shankhpusphi and Brahmi in our laboratory as per WHO guidelines. The
protocol involves investigation of physicochemical stability of the selected formulations. Stability
samples were analysed quantitatively using validated HPLC taking commercially available marker
compounds including scopoletin and asiatic acid. Wide variation in content of both the markers for each
batch of the formulation revealed wide batch to batch variation in marker content on exposure to the
accelerated stability study. Further studies are in progress to establish appropriate storage conditions and
shelf life of the product.

August 22, 2014


PCEU-30

INVESTIGATIONS ON SOLUBILITY ENHANCEMENT VIA SOLID DISPERSIONS
USING MODIFIED NOVEL CARRIERS

Ramika Hardatt*, Kanav Midha, Manju Nagpal, Sandeep Arora
Chitkara College of Pharmacy, Chitkara University,
Chandigarh-Patiala National Highway, Rajpura -140401
E-mail: ramikahardatt25@gmail.com

Abstract:
The rapid advancements intechnology led to the use of modified or novel excipients in novel
dosage forms to accomplishspecific functions (modulate the drug release, stability andimproved
bioavailability, enhancement of patient acceptability). New and improved excipients continue to
be developed to meet the needs of advanced drugdelivery system.Improvement in solubility of
most of new chemical entities (NCEs) is one of major research area where solid dispersion
technique proved to be widely accepted. Solid dispersions are the group of solid products
consisting of different components, a hydrophilic matrix and a hydrophobic drug. Both novel
synthetic and modified natural polymers are being exploited for improvement of solubility. The
natural polymers are preferred now days for pharmaceutical applications as they are
economical,readily available, low cost, non-toxic and capable of chemical modifications. Though
natural gums are being used for sustaining the drug release due to high viscosity; the potential of
modified gums (guar gum, gum karaya, locust bean gum etc.) has also been suitably reported for
poorly soluble drugs(atorvastatin, glimepiride, Licnofelacetc). Moreover, the utility of skimmed
milk powder has been evidenced for solubility enhancement via formulating the solid dispersions
(atorvastatin, valsartan).Soluplus (copolymer of polyvinylcaprolactum-polyvinylAcetate-
polyethyleneglycol) is also being used as a synthetic carrier for solubility enhancement of
Valsartan.Current review highlights latest research on solubility enhancement of various drugs
using modified forms of gums and other carriers by solid dispersion technique.

PCEU-31

SELF EMULSIFYING SOLUTIONS: IMPROVING BIOAVAILABILITY OF BCS
CLASS II DRUGS

Divya & Deepak Kaushik
Department Of Pharmaceutical Sciences, Maharshi Dayanand University,
Rohtak-124001 Haryana (INDIA)

ABSTRACT
Oral route is the easiest and most convenient route for drug administration.The major problem in
oral drug formulations is low and erratic bioavailability, which mainly results from poor aqueous
solubility.Self-emulsifying drug delivery systems(SEDDS) are mainly used in improving the oral
August 22, 2014


bioavailability of poorly water soluble and lipophilic drugs. SEDDSs belongs to lipid
formulations, and size range is from 100nm (SEDDs) to less than 50nm (SMEDDs) and contains
a isotropic mixtures of oils, surfactants, and co-surfactants, which are emulsified in aqueous
media under conditions of gentle stirring. The principal characteristic of these systems is their
ability to form fine oil-in-water (o/w) emulsions or micro-emulsions upon mild agitation
following dilution by an aqueous phase. For lipophilic drugs, which have dissolution rate-limited
absorption.SEDDS may be a promising strategy to improve the rate and extent of oral
absorption,digestive motility that would be encountered in the gastro-intestinal (GI) tract.The
theory behind dissolution rate improvement by means of SEDDS is the spontaneous
development of the emulsion in the gastrointestinal tract with mild agitation provided by gastric
mobility, which presents the drug in solubilized form, and the small size of the formed droplet
provides a large interfacial surface area for drug absorption. Due to its small globule size, the
micro/nanoemulsifed drug can easily be absorbed through lymphatic pathways, thereby
bypassing the hepatic first-pass effect.

PCEU-33

PRONIOSOMES:-A PREFERABLE CARRIER FOR DRUG DELIVERY SYSTEM

Neha khar*, Lokesh Sadana, Surender Verma

Institute of Pharmaceutical Sciences,Kurukshetra University,Kurukshetra
E-mail:nehakhar08@gmail.com

Abstract
Design and development of novel drug delivery system has two prerequisites. First, it should
deliver the drug in accordance with a pre-determined rate and secondly it should release
therapeutically effective amount of drug at the site of action. So, presently some recent studies
have been evaluated that proniosomal system can be used and thus received a great attention in
drug delivery application as well as in pharmaceutical research. In order to minimise the
problems associated noisome physical stability such as aggregation, fusion and leaking; a dry
product preparations can be prepared known as proniosomes. Proniosomes are dry formulations
using suitable carrier coated with non ionic surfactant and can be converted into niosomes
immediately before use by the hydration of non ionic surfactant. Anti-inflammatory action of
certain drugs such as ketorolac via proniosomes gel formulation show better solubility than
conventional niosomes. Niosomes prepared from proniosomes are used in the treatment of
leishmaniasis, and in oncology etc. Recent research has lead to the use of in vitro tests to waive
additional in vivo bioequivalence studies for some pharmaceutical products (i.e., biowaiver).
Efforts are being made to extend biowaivers to certain Class II and III products, which would
represent more than 50% of all drugs coming to the market . Proniosomes are better route for
novel drug delivery system as compared to conventional means.The vesicular proniosomal
powder encapsulating doxycycline hydrochloride (DH) and metronidazole (MT) combination
therapy was developed using different types of spans, cholesterol (CH) and maltodex . The
results obtained indicate that differences in drug dissolution observed in vitro were not reflected
August 22, 2014


in vivo. Such data support that BCS Class III drugs are eligible biowaiver candidates, even if a
very rapid dissolution criterion is not fulfilled.

PCEU-34

FORCED DEGRADATION STUDIES ON AZILSARTAN

Rami Rani,* Dhiraj Kaushik and Gulshan Bansal
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala
147002, India.

Abstract
Forced degradation study of an angiotensin receptor blocker azilsartan was carried out
under hydrolytic (acid, alkali and water), oxidative (H
2
O
2
), dry heat and photolytic
conditions. An isocratic HPLC method was developed and optimized for the separation
of all degradation products formed under varied stress conditions. The drug showed
degradation in acid, base and water hydrolysis and also underwent photo-degradation in
acid, alkali and water medium. In total six degradation products (I-IV) were formed. Out
of the six degradation products detected in LC-UV chromatogram, five (II-VI) were
detected in LC-MS chromatogram. The major degradation products were characterized
on the basis of their mass fragmentation pattern through LC-MS-TOF and MS
n
studies.
These were characterized as 2-ethoxy-3H-benzoimidazole-4-carboxylic acid (Product II),
2-hydroxy-3-[2-(5-oxo-4,5-dihydro-[1,2,4]oxadiazol-4-ylmethyl]-3H-benzoimidazole-4-
carboxylic acid (ProductIII),3-[2-(1H-diazirin-3-yl)-biphenyl]-4-ylmethyl]-2-ethoxy-
3Hbenzoimidazole-4-carboxylic acid (Product IV), 3-[4-(2-ethoxy-benzoimidazol-1-
ylmethyl)-biphenyl-2-yl]-4H-[1,2,4]oxadiazol-5-one (Product V) and 2-ethoxy-3-(5-oxa-
6-aza-dibenzo[a,c]cyclohepten-3-ylmethyl)-3H-benzoimidazole-4-carboxylic acid
(Product VI). Based on the stability of most stable carbocation and literature review, the
most probable degradation mechanism was proposed.

PCEU-35

FORCED DEGRADATION STUDIES ON FLUPIRITINE MALEATE

Vaneetpal Khurana,* Jasmeen Kaur and Gulshan Bansal*
147002, India

Abstract
Forced degradation study of flupiritine maleate was carried out under the conditions of
hydrolysis (acid, alkali and water), oxidation (H
2
O
2
), dry heat and photolysis. An
isocratic HPLC method was developed and optimized for the separation of all
August 22, 2014


degradation products formed in different stress conditions. In total ten degradation
products (I-X) were formed. The drug showed degradation in acid, base and water
hydrolysis and also in photo-degradation in acid, alkali and water medium. The major
degradation products were characterized based on their mass fragmentation pattern
through LC-MS-TOF and MS
n
studies. However, only six products were ionized in LC-
MS chromatogram. Out of the six products, three (VI, VII and X) were characterized as
3-[2-amino-3-(3-amino-6-fluoro-1,2-dihydroisoquinolin-4-yl)-5-hydroxypent-2-
enoylcarbamoyl] acrylic acid, 5-(4-fluoro-phenyl)-5-(6-hydroxy-5-
methoxycarbonylamino-pyridin-2-ylamino)-4-oxo-pent-2-enoic acid and N-(4-
fluorobenzyl)-3-isocyanato-pyridine-2,6-diamine, respectively and the most probable
degradation mechanism was proposed.

PCEU-36

DIFFERENT METHODS OF ENHANCEMENT OF SOLUBILIZATION AND BIOAVAILABILITY
OF POORLY SOLUBLE DRUGS

Ankita Bhardwaj and Arun Nanda
Department of pceutical sciences, Maharishi Dayanand University, Rohtak
Email: anki174303@gmail.com

ABSTRACT
Solubility, the phenomenon of dissolution of solute in solvent to give a homogenous system, is
one of the important parameters to achieve desired concentration of drug in systemic circulation
for desired pharmacological response. Low aqueous solubility is the major problem encountered
with formulation development of new chemical entities as well as for the generic development.
The insufficient dissolution rate of the drug is the limiting factor in the oral bioavailability of
poorly water soluble compounds. This review discusses BCS classification, carriers for solubility
enhancement and different techniques for solubility enhancement. Various techniques are used
for the enhancement of the solubility of poorly soluble drugs which include micronization,
nanonization, sonocrystallization, supercritical fluid method, spray freezing into liquid and
lyophilization, evaporative precipitation into aqueous solution, use of surfactant, use of co-
solvent, hydrotropy method, use of salt forms, solvent deposition, solubilizing agents,
modification of the crystal habit, co-crystallisation, complexation and drug dispersion in carriers.
Selection of solubility improving method depends on drug property, site of absorption, and
required dosage form characteristics. With the advent of combinatorial chemistry and a high
throughput screening, it has now become possible to improve the aqueous solubility of a number
of otherwise poorly water soluble compounds. This research work aims to describe the
techniques of solubilization for the attainment of effective absorption with improved
bioavailability.

August 22, 2014


PCEU-37

NANOTECHNOLOGY FOR CANCER TREATMENT

Neeta* & Harish Dureja
Faculty of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India
Emai: neetasolanki86@gmail.com

Abstract
Recent developments in nanotechnology have provided researchers with new tools for cancer
treatment. Frequent challenges encountered by cancer therapies include nonspecific systemic
distribution of antitumor agents, inadequate drug concentrations reaching the tumor, and the
limited ability to monitor therapeutic responses. Cancer nanotherapeutics are rapidly progressing
and are being implemented to solve several limitations of conventional drug delivery systems
such as nonspecific biodistribution and targeting, lack of water solubility, poor oral
bioavailability, and low therapeutic indices. They are able to carry loaded active drugs to cancer
cells by selectively using the unique pathophysiology of tumors, such as their enhanced
permeability and retention effect and the tumor microenvironment. In this review, attempt has
been made to highlight the use of nanoparticulate drug delivery systems for cancer treatment.

PCEU-39

NANOTECHNOLOGY AS A THERAPEUTIC TOOL TO
COMBAT MICROBIAL RESISTANCE

Priya* & Dr. Harish Dureja
Department Of Pceutical Sciences, Maharishi Dayanand University,Rohtak
*Email :- Priyadhiman062@gmail.com

Abstract
Nano- denotes nanometer (10
9
meter). An emerging integrative approach to treating infectious
diseases is using nanoparticle (NP) forms of traditional and alternative medicines. Following
types of nanoparticles are available like: Nitric oxide-releasing nanoparticles (NO NPs),
chitosan-containing nanoparticles (chitosan NPs), and metal-containing nanoparticles all use
multiple mechanisms simultaneously to combat microbes, thereby making development of
resistance to these nanoparticles unlikely. Nanoparticles can overcome existing drug resistance
mechanisms, including decreased uptake and increased efflux of drug from the microbial cell,
biofilm formation, and intracellular bacteria.Nanocrystalline silver coating effective against
methicillin resistant staphylococcus aureus ( MRSA), vancomycin resistant enterococci (VRE),
antibiotic-resistant strains Pseudomonas and fungi. Used as an antimicrobial wound dressings in
first and second degree burns. Combining antibiotics with nanoparticles also restores their ability
to destroy bacteria that have acquired resistance to them. Likewise, combining nanoparticles with
antimicrobial peptides and essential oils generates genuine synergy against bacterial resistance.
August 22, 2014


PCEU-40

INSULIN LOADED BILOSOMES FOR TREATMENT OF DIABETES MELLITUS

Daisy Arora*, Anu Devi, Bharat Khurana
Doon Valley Institute of Phamacy and Medicine, Karnal (Haryana)
E-Mail: daisyarora86@gmail.com

Abstract:
Diabetes is a challenging problem for public health worldwide which can be managed and
controlled through proper medication. Insulin is a protein hormone which promotes the efficient
storage and utilization of the fuel molecules by controlling the transport of metabolites and ions
across the cell membrane. Due to the many drawbacks of the subcutaneous route for
administration of insulin, alternative modes of administering insulin continue to attract
considerable research interest. Oral route is more beneficial than parenteral route because it
improves the patient compliance and reduces infusion related side effects. Various delivery
systems are in study for oral delivery of insulin. For example Bilosomes which are lipid-based
vesicle system that consist of non-ionic amphiphiles forming a closed bilayer structure and
incorporating bile salts. Bile salts have been shown to stabilize the membrane against the
detrimental effects of bile acids in gastrointestinal tract. In the present work insulin loaded
Bilosomes were developed and characterized for selective and preferential localization of insulin
via oral route for the treatment of diabetes. The formulation was characterized for their shape and
surface morphology which found to be optimum for oral use. Insulin released from the bilosomes
was characterized by slow release of 28.72% for 4 hrs and followed by controlled release. The
developed delivery systems and their oral mode of delivery overcome the disadvantages of the
classic parenteral delivery. The present investigation is seminal however; elaborative studies and
clinical trials are warranted to assess real potential of the developed carrier systems.

PCEU-41

DEVELOPMENT AND CHARACTERIZATION OF AMPHOTERICIN B LOADED
ETHOSOMES FOR THE ENHANCED TREATMENT OF TOPICAL FUNGAL
INFECTIONS

Bharat Khurana*, Asha Rani, Daisy Arora
Doon Valley Institute of Phamacy and Medicine, Karnal (Haryana)
E-Mail: bharatkhurana85@gmail.com

Abstract:
Fungal skin infections are caused by dermatophytes-types of fungi that cause skin, nail and hair
infections. Fungi are ubiquitous organisms that are capable of colonizing almost any
environment, including all human beings. Infection may be superficial, deep or opportunistic.
Topical administration of antifungal drug remains to be the most efficient and cost effective
strategy. In the present study Amphotericin B loaded ethosomes were developed. Amphotericin
August 22, 2014


B was used because it is broad spectrum antifungal and also effective against all common
dermatophytes viz. epidermophyton, microsporum, trichophyton. Topical preparations are used
for the localized effects at the site of their application by virtue of drug penetration into the
underlying layers of skin or mucous membranes. Conventional formulation of antifungal drug
Amphotericin B i.e. Fungizone cream are being used for treatment of skin infections but these
formulation resulted in severe blistering, itching, redness, peeling, dryness, or irritation of the
skin and also failed to achieve mycological eradication. While novel delivery system i.e.
ethosomes enhanced the penetration of bioactive moiety into the skin, localizes the drug at the
site of action.

PCEU-42

DEGRADATION PRODUCT PROFILING OF HYDROXYCHLOROQUINE USING
MS
N
, LC-ESI-MS-TOF AND LC-PDA TECHNIQUES

Gurmeet Singh, Balraj Saini, Gulshan Bansal
Department of Pharmaceutical Sciences and Drug Research,
Punjabi University, Patiala 147002, INDIA
Abstract
The present study reports the degradation behavior of hydroxychloroquine (HCQ) under the
conditions of hydrolysis (acid, alkali and water), oxidation (30% H
2
O
2
), dry heat and photolysis
(UV-VIS light) in accordance with the ICH guidelines. HCQ and all six degradation products
formed under alkaline photolytic conditions were optimally resolved on an XTerra
RP18
column with mobile phase composed of methanol, acetonitrile and ammonium formate buffer
(20mM, pH 4.5) in an isocratic mode. The drug was stable in all other forced condition. The
products were characterized through +ESI-MS
n
and LC-ESI-MS-TOF and LC-PDA data of the
drug as well as degradation products. The products I-IV were characterized as N-oxide HCQ,
dechlorinated HCQ, N-desethylated HCQ and N-dealkylated HCQ. The product I and VI were not
characterized due to limited data. This library of HCQ degradation products provides a foundation
for further product development and stability monitoring.

PCEU-43

NANOPARTICLES FOR DRUG DELIVERY TO THE BRAIN

Gourav*, Rakesh pahwa
Institute of pharmaceutical sciences KUK-136009
Email: payalgoel.bch@gmail.com

Abatract
There is extensive research currently being done in the field of nanoparticle drug delivery systm
for brain. One popular diseases being studied in neuroscience today is Alzheimers disease. A
August 22, 2014


few Alzheimer s drugs that have been studied in particular are rivastigmine ,tacrine, piperine
and circumin.PBCA and PLGA nanoparticles were used as delivery system for these drugs. This
possibly suggest that nanoparticles could provide a promising solution to how these drugs could
cross the BBB. One factor though that still must be considered and accounted for is nanoparticle
accumulation in the body.with long term and frequent injections that are often required to treat
chronic diseases such as Alzheimers disease, with the aid of nanoparticle delivery system
certain drugs now cross BBB and exhibit lower toxicity and decrease adverse effects throughout
the body.

PCEU-44

FORMULATION AND EVALUATION OF ENTERIC COATED
MULTIPARTICULATE SYSTEMS OF ESOMEPRAZOLE MAGNESIUM

S.K.Gupta*
1
and I.J.Singhvi
2
1
Vidyasthali Inst. of Tech. Science and Management, Jaipur (Raj.)
2
Pacific College of Pharmacy, Udaipur (Raj.)
E-mail: garg_s.kumar@yahoo.com

Abstract:
Esomeprazole Magnesium was used as a model drug in the present investigation. It is a
benzoimidazole derivative belonging to a group of proton pump inhibitors. It inhibits gastric acid
secretion at the final step of the acid secretary pathway and thus reduces basal and stimulated
gastric acid secretion irrespective of stimulus. Premature drug release from enteric coated dosage
forms in the stomach potentially results in the degradation of the drug or may cause irritation of
the gastric mucosa. This can be reduced with coated pellets because of a more rapid transit time
compared to enteric coated tablets. The Objective of present work is to develop, formulate and
evaluate the deleyed release pellets of Esomeprazole Magnesium by using different coating
processes for the treatment of dyspepsia, peptic ulcer disease (PUD), gastro esophageal reflux
disease (GORD/GERD) and Zollinger-Ellison syndrome. The Delayed release Pellets was
formulated using Esomeprazole Magnesium & MCC in 30:70 ratios. Microcrystalline cellulose
(Avicel) pH101, Kollicoat Protect and Eudragit L30D55 were taken as the formulation variables
for optimizing to keep round shape of pellets and percentage release of drug. The pellets were
evaluated for Physical characterization, Assay, Sizing, Aspect ratio, density, SEM, In-vitro drug
release. Thus, we can conclude that delayed release multi unit particulate system of
Esomeprazole Magnesium by pelletization techniques will serve as a promising approach for the
delivery of the drugs.

PCEU-45

QUALITY AND PERFORMANCE ENHANCEMENT OF NSAIDS ANTIPLATELET
AGENT IN GASTRORESISTANT FORMULATION

August 22, 2014


S.K.Gupta*
1
and I.J.Singhvi
2

1
Vidyasthali Inst. of Tech. Science and Management, Jaipur (Raj.)
2
Pacific College of Pharmacy, Udaipur (Raj.)
E-mail: garg_s.kumar@yahoo.com

Abstract:
The tablet dosage form accounts for approximately 70% of all dosage forms on the market. A
polymer coating is often applied to make the tablet smoother and easier to swallow, to control
the release rate of the active ingredient, to make it more resistant to the environment (extending
its shelf life) or to enhance the tablet's appearance. The main aim of this work is to formulate a
delayed release stable enteric coated tablet of Aspirin to prevent the clot formation in the blood
and used in the disease like Heart attack, Atherosclerosis and increase the stability of tablet by
formulating various batches. Under Preformulation study the organoleptic properties were
complied with British pharmacopoeia (BP) specification. Identification of aspirin carried out by
Infra Red (IR) spectral analysis. Drug excipients compatibility study performed at 40
o
C /75%
RH of 15 days. Micro crystalline cellulose, Pregelatinized starch and Sodium lauryl sulphate
were used to prepare a blend for direct compression method. To protect the drug form
degradation in acidic environment, final tablet formulation F6 coated with pH dependant
solubility polymer Insta Moist Shield White (IC-G-M-10007). Drug content was found to be
between 90 % to 110 % and it was within the limits. Finally it is concluded that, Aspirin Enteric
Coated 75 mg tablets were prepared by direct compression techniques, showed promising results
when compared with marketed drug.

PCEU-46

NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF
PARACETAMOL AND PROMETHAZINE IN SYRUP FORMULATIONS.

Akash Jain
1
, Jasmine
1*
, Vipin Saini
1

M. M. College of Pharmacy, M.M.University, Mullana-Ambala, Haryana-133207, India
e-mail: akash2911@gmail.com

ABSTRACT:
Background: Paracetamol with Promethazine HCl is one of the most popular over the counter
drug which is used as antipyretic and antiemetic. A novel, simple, precise, less time consuming,
economical and accurate reverse phase HPLC method has been proposed for their simultaneous
estimation in which chromatographic separation was achieved on Kromasil C
18
column with
mobile phase consisting mixture of Water: Methanol: Acetic acid (79:20:1 v/v) at flow rate of 1
mL/min and detector wavelength 249 nm.
Results: Retention time was found to be 3.564 and 5.620 minutes for Paracetamol and
Promethazine respectively and linearity was found to be in concentration range of 10-50 g/ml
for both drugs. Tailing factor is within the range (< 1) and number of theoretical plates is more
than 2500. The proposed method was validated for specificity, linearity, accuracy and precision
August 22, 2014


according to ICH guidelines. % RSD was found to be less than 2 indicating the method to be
precise and accurate.
Conclusion: The results indicate that the proposed method can be used for the routine analysis
of Paracetamol and Promethazine in combined dosage form without interference of the
excipients present in formulation.

PCEU-47

NANOPARTICLES: NOVEL DRUG DELIVERY SYSTEM FOR CANCER
Komal*, Rakesh Pahwa
Institute Of Pharmaceutical Sciences, Kurukshetra University Kurukshetra
E- Mail: komaltaya508@gmail.com

Abstract
A nanoparticle is a small object that behaves as a whole unit in terms of its transport properties.
Nanoparticles size ranges from 100 and 2500 nanometer. Technological advantages of
nanoparticles are targeting drugs to specific sites in the body, reduced toxicity, and enhanced
uptake of antitumour agents. Compared to direct drug delivery through a carrier can increase the
efficacy of a drug, but decrease the side effects and tumor specific targeting. The small colloidal
carriers like biodegradable and drug substances can be incorporated normally by a process of
surface adsorption. These biocompatible nanoparticles, with an enlarged surface area volume
ratio can overcome cellular and non cellular based mechanisms of resistance and increase the
selectivity of drug towards cancer cells, while reducing their toxicity towards normal tissues. The
polyalkylcyanoacrylate nanoparticles have been studied in recent years as a possible means of
targeting drugs to specific sites in the body, with particular emphasis to cancer chemotherapy.
Some anticancer drugs those are either entrapped or adsorbed onto polyalkylcyanoacrylate
nanoparticles. Other biodegradable polymers like polyvinylpyrrolidine are also investigated for
drug delivery in cancer therapy. These include dexamethasone in poly(lacitde-co-glycolide)
nanoparticles and taxol in polyvinyl pyrrolidone nanoparticles.

PCEU-48

TRANSDERMAL DRUG DELIVERY SYSTEM

Rajni Devi*, Mr. Tarun Kumar Sharma, Priya Sharma
LR College of Pharmacy, Solan.
Email:PHARMA.RAJNI06@gmail.com

Abstract
Transdermal drug delivery system defined as the administration of therapeutic drugs through the
skin. Transdermal delivery not only provides controlled, constant administration of the drug, but
also allows continuous input of drugs with short biological half lives, and eliminates pulsed entry
August 22, 2014


into systemic circulation, which often causes undesirable side effects. The common ingredients
which are used for transdermal drug delivery are as drug, liners, adhesive, permeation enhancers
and backing layer. There are different types of transdermal patches which are generally used in
transdermal drug delivery such as single layer drug in adhesive, multilayer drug in adhesive,
vapour patch, reservoir system, matrix system, microreservoir system. Transdermal drug delivery
provides a means to sustain drug release as well as reduce the intensity of action and thus reduce
the side effects associated with its oral therapy. The drug delivery is designed to support the
passage of drug substances from the surface of skin, through its various layers and into the
systemic circulation. Taking into account the advantages of transdermal drug delivery system, it
can be considered a perfect alternative for drugs whose enteral and parenteral dosage forms
having drawbacks in performances and also in patient compliance. Many drugs with a
hydrophilic structure permeate the skin too slowly to be of therapeutic benefit. Drugs with a
lipophillic character, however, are better suited for transdermal delivery.

PCEU-49

NATURAL THERAPY BASED ON NATURAL POLYMERS USED FOR COLONIC
DISEASES
Mohini Sharma*, Surender Verma
Email id: leomanu1992@gmail.com

Abstract
Colonic drug delivery has gained increased importance for the delivery of the drugs for the
treatment of local diseases associated with the colon. To achieve successful colonic delivery,
colon targeting is of prime importance. Various natural therapies are available for the treatment
of colonic diseases like ulcerative colitis, IBS, colon cancer, diverticulitis, etc based on natural
polymers such as guar gum, pectin, dextran, chitosan, inulin, amylose, etc. Natural polymers are
used as excipients in formulation of various dosage forms and are obtained from various sources.
Interest in these biodegradable polymers is increasing day by day because these are safe, non-
toxic, and economic and are chemically compatible with the other excipients in the formulation.
Non- starch, linear polysaccharides remain intact in the physiological environment of the
stomach and the small intestine, but are degraded by the bacterial inhabitants of human colon
which make them potentially successful in targeted delivery systems to the colon. Certain single
herbs used in the treatment of colonic diseases are Aloe Vera, Curcuma longa, Curcuma
xanthorrhiza, Cynara scolymus, Slippery elm, Tormentil extracts, Bromelain, Psyllium, etc.
There are certain preparations such as Boswellia serrata nanoparticles, curcumin microspheres
available in the market to treat colon cancer. Various approaches that can be exploited to target
the release of drug to colon include prodrug formation, coating with ph sensitive polymers and
biodegradable polymers, embedding in biodegradable matrices, hydrogel, time release systems,
osmotic and bioadhesive systems. However, recently continues efforts have been taken on
designing colon-specific delivery systems with improved site specificity and versatile drug
release kinetics.

August 22, 2014


PCEU-50

Development & Characterization of Losartan loaded Floating Gastroretentive
Microspheres

Ashish single
1
, S. K. Singh
2
, D. N. Mishra
2
1
JCDM College of Pharmacy, Sirsa-125055 (Haryana)
2
Deptt. of Pharmaceutics, G.J.U.S. & T., Hisar-125001(Haryana)
E-mail ID- ashishsingla2009@yahoo.co.in

Abstract
The objective of present research work was to formulate and evaluate in-vitro performances of
floating gastroretentive microspheres of Losartan for potential use of treating hypertension by
blocking Angiotensin receptors. Losartan floating microspheres containing Eudragit RS 100 &
RL 100 polymer were prepared by solvent evaporation technique with different concentration of
these polymers. The formulation factors like: drug: polymer ratio, concentration of emulsifier,
aqueous: oil phase ratio, viscosity of aqueous phase, stirring speed and stirring time on particle
size, drug encapsulation, process yield and drug release behaviour were studied. Microspheres
were found spherical, free flowing, high percentage drug entrapment efficiency and floating
property. Particle size was determined by optical micrometer and average particle size was found
in the range of 80-120 micrometer for all batches. The optimized batch exhibited a high drug
entrapment efficiency of 65 %, high percentage yield i.e. 85 % and high floating duration of 11
hrs. The drug was also showing sustained drug release pattern for more than 24 hours. The
release profiles of all Microspheres formulations best fitted into Higuchi model which described
drug release from homogeneous & granular matrix system. In conclusion, the results of the
present investigation showed that floating microsphere can be promising delivery system for
improvement of bioavailability of low bioavailable drug like Losartan which have narrow
absorption window in upper part of GIT.

PCEU-51

AN OVERVIEW ON SELF-ASSEMBLED NANOPARTICULATE
CARRIER SYSTEM: AQUASOMES

Swati Aggarwal*
1
, Deepa Batra
1
, Kunwar Singh
1
, Vaibhav Gupta
1

1
Doon Valley Institute of Pharmacy & Medicine, Karnal, Haryana

ABSTRACT
In the past few years many approaches were tried for the improvement of the drug delivery.
Nanoparticulate carrier system contributes one of the self assembling approaches for the delivery
of pharmaceutical agents. Aquasomes are one of the most recently developed nanoparticulate
carrier system for the delivery of bioactive molecules like peptide, protein, hormones, antigens
and genes to specific sites. Instead of being simple nanoparticles these are three layered self
August 22, 2014


assembled structures, comprised of a solid phase nanocrystalline core coated with oligomeric
film to which biochemically active molecules are adsorbed with or without modification. They
contain nanocrystalline calcium phosphate or ceramic diamond covered by a glassy
polyhydroxyl oligomeric film. Three types of core materials are mainly used for producing
aquasomes: tin oxide, nanocrystalline carbon ceramics (diamonds) and brushite (calcium
phosphate dihydrate). Aquasomes are spherical (5-925nm) particles and are called as bodies of
water". Their water like properties protects and preserves fragile biological molecules. Properties
like protection and preservation of fragile biological molecules, conformational integrity, and
surface exposure made it as a successful carrier system for bioactive molecules like peptide,
protein, hormones, antigens and genes to specific sites. Enzyme activity and sensitivity towards
molecular conformation made aquasome as a novel carrier for enzymes like DNA and pigment/
dyes. This report reviews the principles of self assembly, the challenges of maintaining both the
conformational integrity and biochemical activity of immobilized surface pairs, and the
convergence of these principles into a single functional composition.

PCEU-52

SCREENING OF DIFFERENT GROWTH MEDIUM FOR EXTRACELLULAR
BACTERIAL SYNTHESIS OF SILVER NANOPARTICLES: NEW METHOD

Divya Bhatia and Deepak Kumar Malik
Department of Biotechnology Engineering,UIET, Kurukshetra University, Kurukshetra-
136119,Haryana, India, Email id: divyabhatia228@gmail.com

Abstract
In the recent past, paramount importance is given to research in the field of nanotechnology
owing to its applications in various fields. Nanoparticles have been known to be used for
numerous physical, biological and pharmaceutical applications. The synthesis of silver
nanoparticles (AgNPs) has received considerable attention with their potential applications in
various life sciences related applications. Silver nanoparticles (SNPs) have extensive
applications in civil, therapeutic and industrial areas as catalyst, cryogenic superconductor,
biosensor, microelectronic and bacteriostatic materials. Currently, there is a growing need to
develop environmentally benevolent nanoparticle synthesis processes that do not use toxic
chemicals in the synthesis protocol. Among the various known synthesis methods, biosynthesis
of silver nanoparticles is preferred as it is environmentally safe, low cost and less toxic.In the
present study, we have demonstrated the extracellular biosynthesis of silver nanoparticles by
bacterial strains isolated from a soil sample collected from the metal contaminated site in India.
Biosynthesis of silver nanoparticles was observed with in only few minutes under bright
conditions. Nanoparticles formation was not observed under dark conditions even after 4 days.
Some other studies have reported the extracellular biosynthesis of AgNPs by using nutrient broth
medium, but formation of AgNPs was observed even when only the nutrient broth was added to
silver nitrate solution. In the present study, BSM was used as a culture medium and synthesis of
silver nanoparticles was not observed when only BSM was added to silver nitrate solution. So,
this is the first study that reports the real biosynthesis of AgNPs. The nanoparticles of silver were
August 22, 2014


characterized based on UV-Visible spectroscopy. The silver nanoparticles exhibited maximum
absorbance at 420 nm in UVVisible spectroscopy. Further characterization of AgNPs will be
done using TEM, SEM and XRD.
.

PCEU-53

DENDRIMERS: PROMISING NANODEVICES FOR TARGETED DRUG DELIVERY

Anshul Garg
*1
and Rakesh Pahwa
1
1
*
E-mail:- anshulgarg369@gmail.com

Abstract
Dendrimers are a family of nanosized, three-dimensional polymers characterized by unique tree-
like branching architecture and compact spherical geometry in solution. Attractive features such
as narrow polydispersity index, excellent control over molecular structure, availability of
multiple functional groups at the periphery and cavities in the interior distinguish them amongst
the polymers. The biological fate of dendrimer mediated drugs can be significantly altered based
on their intrinsic physicochemical properties including the hydrophilicity of the unit molecules,
particle size, surface charge and modification. This approach certainly promises a reliable, safe,
selective and precise method of targeted drug delivery. These are being adopted as tool for
targeted drug delivery in many avenues including drug delivery, immunology and the
development of vaccines, antimicrobials and antivirals. Hence, the present endeavour focuses on
various fundamental of dendrimers and their utilization as drug delivery in the treatment of
various disorders.
Keywords:-Dendrimers, Drug Delivery, Nano Carriers

PCEU-54

NIOSOMES: A POTENTIAL VESICULAR DRUG DELIVERY SYSTEM

Yugam Taneja
*1
and Rakesh Pahwa
1

1
*
E-mail:-yugamtaneja29@gmail.com

ABSTRACT
Niosomes are non-ionic surfactant vesicles obtained on hydration of synthetic nonionic
surfactants with or without incorporation of cholesterol or their lipids. These are vesicular drug
delivery systems that can be utilized as carriers of both amphiphilic and lipophilic drugs. They
are biodegradable, biocompatible nonimmunogenic and exhibit flexibility in their structural
characterization. Niosomes are preferred over liposomes because the former exhibit high
chemical stability and economy. Niosomes have been widely evaluated for controlled release and
August 22, 2014


targeted delivery for the treatment of cancer, viral infections and other microbial diseases.
Niosomes can prolong the circulation of the entrapped drug in body. Encapsulation of drug in
vesicular system can be predicted to prolong the existence of drug in the systemic circulation and
enhance penetration into target tissue, perhaps reduce toxicity if selective uptake can be
achieved. Niosomes also serve as better aid in diagnostic imaging and vaccine adjuvant.The
present endeavour focuses on the advantages, disadvantages, preparation methods, factors
affecting, characterization aspects, in-vitro methods, drug release kinetics and applications of
niosomes.
Keywords: Niosomes, Pharmaceutical applications, Methods, Characterization aspects

PCEU-55

ANTIMICROBIAL ACTIVITY OF GREEN SYNTHESIS SILVER NANOPARTICLES
AGAINST HUMAN PATHOGENS

Swati Kodan and Deepak Kumar Malik
Department of Biotechnology Engineering,UIET, Kurukshetra University, Kurukshetra-
136119,Haryana, India, Email id: divyabhatia228@gmail.com

Abstract
Synthesis of nanoparticles has been the subject of a lot of studies due to its commercial demands
and wide applicability in various areas. Nanotechnology is an emerging field, nanoparticles is
helpful in investigation and regulation at cell level interaction between synthetic and biological
materials. In many areas of human science these materials are superior and indispensable due to
its unique size dependent.The chemical and physical methods of silver nanoparticles synthesis
were being followed over the decades, but they are found to be expensive and the use of various
toxic chemicals for their synthesis makes the biological synthesis more preferred option. The
easy availability, nontoxic nature and quicker synthesis make plant extracts ideal choice for
nanosilver synthesis. The bacterial, fungal, plant extract sources can be used for nanosilver
synthesis. The plants (Lawsonia inermis stem, Andrographis paniculata leaf, Ocimum
tenuiflorum root, Piper longum stem) play an important role in the reduction and stabilization of
silver. The green synthesized AgNPs showed antibacterial activity on both bacteria and fungus
i.e. Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Bacillus subtilis,
Candida albicans and Candida glabrata. The use of silver nanoparticles in drug delivery
systems might be the future thrust in the field of medicine.

August 22, 2014


PCEU-56

NEW HORIZONS IN THE VISTAS OF MICROEMULSIONS FOR IMPROVED DRUG
DELIVERY

Parveen Kumar
*1
andRakesh Pahwa
1
1
Institute of Pharmaceutical Sciences, KurukshetraUniversity, Kurukshetra
*
E-mail:-puniaparveen92@gmail.com

ABSTRACT
Micro-emulsions are excellent candidate as a potential drug delivery system because of their
improved drug solubilization,longshelf life, ease of preparation and administration. Association
of drugs with carriers is normally non-covalent basedon collective strength of weak binding
forces. They are clear, stable, isotropic mixtures of oil, water and surfactant in combination with
a co-surfactant.Micro-emulsions are readily distinguished from normal emulsions by their
transparency, low viscosityand more fundamentally their thermodynamic stability.These systems
are currently ofinterest to the scientific community worldwide because of their considerable
potential to actas drug delivery vehicles by incorporating a wide range of drug molecules. There
are pharmaceutically acceptable dosage form with clearunderstanding of the micro-emulsion
structure, phase behavior, factors leading toits thermodynamic stability, factors influencing drug
release, the potential uses andlimitations of the micro-emulsion system.
Key-words: Micro-emulsions, Surfactants, Co-surfactants, Thermodynamic stability

PCEU-57

NANOPARTICLES-IN-MICROPARTICLES SYSTEMS: An Overview

Parijat Pandey and Harish Dureja
Department of Pharmaceutical Sciences
Maharshi Dayanand University, Rohtak-124001
parijatpandey98@gmail.com

Abstract:
Nanoparticles-in-Microparticles System (NiMS) can be defined as a novel drug delivery system
that combines particles of nano- and micro- ranges for drug and gene delivery in specific regions
of the body. The drug is dissolved, entrapped, encapsulated or attached to a nanoparticles matrix.
These NiMS can be further useful for the development of new drug-loaded ODTs. The major
goals in designing NiMS as delivery system are to control particles size, surface properties and
release of pharmacologically active agents in order to achieve the site-specific action of the drug
at the therapeutically optimal rate and dose regimen. There are various methods used for
preparation of NiMS such as ionic gelation, emulsion cross-linked method, coacervation/
precipitation, nanoprecipitation, spray-drying, emulsion-droplet coalescence, salting-out method,
sieving method, emulsification diffusion method. The aim of the present study is to highlight the
applications of NiMS in pharmaceutical drug delivery.

August 22, 2014


PCEU-59

RELEVANCE OF VARIOUS TECHNIQUES OF TASTE MASKING FOR BITTER
DRUGS

Himanshu*
1
, Abhinav Singh Rana and Rakesh Pahwa
1
1
*E-mail:- himanshu.kamboj34@gmail.com

Abstract
Acceptability of any drug dosage form generally depends over its taste i.e. mouth sensation.
Drug molecule interacts with taste receptor on the tongue to provide bitter, sweet or other taste
sensation, when they dissolve in saliva. This sensation of taste is the result of signal transduction
from the receptor organs for taste commonly known as taste buds. Taste is one of the most
important aspects of formulation development of oral liquids, disintegrating tablets, chewing
tablets or mouth dissolving tablets in terms of patient compliance. Now, these days most of the
potent drugs that may be cardiac, analgesics, anti-inflammatory, anti-tubercular, antibacterial,
anticoagulant, antiepileptic, diuretics, anti-thyroids, histamine receptor antagonists, oral
vaccines, nutritional agents etc. are bitter in taste. Therefore, it becomes necessary to develop
such dosage form that must be acceptable in taste of patient especially in case of paediatrics and
geriatrics patients as taste masking of bitterness becomes essential. To overcome problem of
bitterness, many techniques have been developed worldwide. These techniques are not only
masking the bitter taste of drug but also enhance the bioavailability and performance of drug
dosage form. It includes adding sugar, use of lipoproteins, numbing of taste buds, granulation,
coating drugs, solid dispersions, viscosity modifier, microencapsulation, multiple emulsion and
salt formation, inclusion and molecular complexes and ion exchange resins which have been
explored by the scientific community to mask the unpleasant taste of the bitter drugs.
Keywords: Mouth sensation, Disintegrating tablets, Microencapsulation, Taste masking

PCEU-60

FORMULATION AND EVALUATION OF LEVOFLOXACIN SUSTAINED RELEASE
TABLET

Komal Goswami*, Vaneeta Chaudhary, Gaurav Khurana, Priti Mehndiratta, Jyoti Dahiya,
Minakshi Gupta,
Shri Baba Mast Nath Institute of Pharmaceutical Sciences and Research, Asthal Bohar.

Abstract
Suitable analytical method based on UV-Visible spectrophotometer was developed for
levofloxacin. Wavelength of 290nm in 0.1N HCl and pH 6.8 buffer was identified. Levofloxacin
sustained release tablets were prepared by using ethyl cellulose, HPMC and HPC as excipients
by wet granulation method. The manufacturing process was standardised and found to be
reproducible. The prepared formulation may reduce the dosing intervals, side effects and
August 22, 2014


increase the efficacy of drug for treating bacterial infections. Evaluation parameters showed
satisfactory results. Formulation containing HPMC showed better release and finalised. It was
found to exhibit satisfactory physico-chemical characteristics and followed zero order kinetics.
Accelerated stability studies for three months showed that tablets were stable. The sustained
release tablets of levofloxacin were developed successfully.

PCEU-61

Preparation and characterization of nanostructured lipid carriers based topical delivery of
diclofenac

Neelam Poonia, Anil Kumar, Deepti Pandita, Viney Lather
JCDM College of Pharmacy, Sirsa-125055, Haryana, India
Email: neelampoonia123@gmail.com

Abstract
Diclofenac, an NSAID, has been recommended orally for the treatment of rheumatoid arthritis
and osteoarthritis. It also has anti-inflammatory, antipyretic and analgesic activity. The oral
administration of diclofenac causes gastrointestinal ulcers and bleeding in chronic use.
Transdermal delivery of diclofenac may avoid these side effects, may help in the better patient
compliance and bypasses first pass metabolism. However, several problems have been reported
with the conventional topical preparations e.g. low uptake due to the barrier function of stratum
corneum. Therefore, an improved diclofenac formulation is desirable which gives high degree of
permeation. Nanostructured lipid carriers (NLCs) have gained more attention for minimizing the
side effects and enhance dermal penetration. In the present research work, Diclofenac sodium
loaded NLCs were prepared by modified solvent injection method for enhancing the dermal
penetration and minimizing side effects associated with its oral delivery. Various variables were
optimized i.e liquid lipid content, amount of surfactant, time of stirring, homogenization speed
and drug content. Diclofenac sodium loaded NLCs having mean particle size of 88.21.6 nm
with lower PDI value 0.1890.043 and high zeta potential value (-28.182.10) were obtained.
Also, DSC analysis confirmed that diclofenac sodium was successfully encapsulated and present
in amorphous state in NLCs. Ex vivo permeation study of Diclofenac sodium loaded NLCs gel
showed biphasic drug release pattern with initial burst release followed by sustained release. The
NLCs subjected to stability studies at 252C/605 RH and 42C for 45 days were found to be
stable. Overall it can be concluded that the developed NLCs have enormous potential for topical
delivery of bioactives.

August 22, 2014


PCEU-62

TARGETED DRUG DELIVERY SYSTEM

Atul Attri
*1
, Priti Mehndiratta
1
, Upma
1
, Jyoti Dahiya
1
, Komal Goswami
1
, Minakshi Gupta
1

1

Abstract
Targeted drug delivery is a method of delivering medication to a patient in a manner that
increases the concentration of the medication in some parts of the body relative to others.
Targeted delivery of drugs, as the name suggests, is to assist the drug molecule to reach
preferably to the desired site. The inherent advantage of this technique has been the reduction in
dose & side effect of the drug. There are different types of drug delivery vehicles, such as
polymeric micelles, liposomes, lipoprotein-based drug carriers, nano-particle drug carriers,
dendrimers, etc. The advantages to the targeted release system is the reduction in the frequency
of the dosages taken by the patient, having a more uniform effect of the drug and reduced
fluctuation in circulating drug levels. The system is based on a method that delivers a certain
amount of a therapeutic agent for a prolonged period of time to a targeted diseased area within
the body. This helps maintain the required plasma and tissue drug levels in the body, thereby
preventing any damage to the healthy tissue via the drug. The most important application of
targeted drug delivery is to treat cancerous tumors. Future of targeted drug delivery is promising
and going to set the new trend in pharmaceutical world.

PCEU-63

FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY
SYSTEM FOR DOMPERIDONE

Pokali Sarabaiah*
1
, Upma
1
, Komal Goswami
1
, Rakesh Kumar Marwaha
2
, Minakshi Gupta
1

1
2
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak

ABSTRACT
Floating drug delivery system for domperidone was prepared to improve the bioavailability of
the drug, reduce the dosing frequency of drug and to increase the residence time in the stomach.
Floating tablets of Domperidone were prepared by employing HPMC polymers, ethyl cellulose
as a hydrophobic floating enhancer, Sodium bicarbonate as a gas-generating agent in
formulations by wet granulation method. The prepared tablets were characterized for physical
properties like bulk density, tapped density, angle of repose, Carrs index, Hausners ratio. All
the blends showed satisfactory properties with formulation F5 showing the best results for all
these parameters. Tablets were evaluated for uniformity of weight, thickness, hardness,
percentage (%) friability and in vitro \release studies. The formulation F5 containing HPMC:
August 22, 2014


ethyl cellulose ratio (1:2) has been found to be the best formulation on the basis of above
evaluation parameters.

PCEU-64

FORMULATION AND EVALUATION OF IMMEDIATE RELEASE TABLET OF
AMLODIPINE

Priti Mehndiratta
*1
, Sunil Chawla
1
, Upma
1
2
, Minakshi Gupta
1

1
2

Abstract
An immediate release dosage form has emerged as an alternative oral dosage form because it
disintegrate rapidly after oral administration with enhanced rate of dissolution. In this study
immediate release tablets of Amlodipine were formulated by direct compression method. Nine
formutions S1-S3, C1-C3, P1-P3 of immediate release oral tablets were prepared by using
different disintegrants ( sodium starch glycolate, croscarmellose sodium, crospovidone) to get
desired release profile. Various evaluation parameters like weight variation, hardness, friability,
thickness, disintegration test and in-vitro drug release studies were performed. The optimized
formulation P3 showed satisfactory hardness (3.40 Kg/cm
2
), friability (0.60), weight (200.77
mg), disintegration time (58 sec.) and stability. The comparison of all the batches prepared by
different concentration of different superdisntegrants showed that batch P3 (1.5 % crospovidone)
is the best batch among all the batches due to maximum drug release (98.83 %).

PCEU-65

MICROPARTICULATE DRUG DELIVERY SYSTEM

Rahul Rathee
*1
, Priti Mehndiratta
1
, Upma
1
, Gaurav Khurana
1
, Komal Goswami
1
, Jyoti Dahiya
1

1

Abstract
Particulate drug delivery systems have become important in experimental pharmaceutics and
clinical medicine. Carrier technology offers an intelligent approach for drug delivery by coupling
the drug to a carrier particle cuch as microsphere, nanoparticles etc. which modulate the release
and absorption characteristics of the drug. Microparticles constitute an important part of this
particulate drug delivery system by virtue of their small size and efficient carrier characteristics.
Microparticles are proving to be a boon in overcoming the difficulties associated with traditional
method of administration. They have been used in vivo to protect the drug entity in the systemic
circulation, restrict access of the drug to the chosen sites and to deliver the drug at a controlled
and sustained rate to the site of action. Various polymers have been used in the formulation of
August 22, 2014


microparticles for drug delivery research to increase therapeutic benefit, while minimizing side
effects. Microparticles were initially developed as a carrier for vaccines and anti-cancer drugs.
Microparticles have been proven to be useful in this manner for the delivery of various active
pharmaceutical ingredients.

PCEU-66

FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY SYSTEM
FOR FAMOTIDINE

Upma
*1
, Sombir Singh
1
, Priti Mehndiratta
1
2
, Minakshi Marwaha
1

1
2

Abstract
Floating drug delivery system of Famotidine was developed for local action in stomach, to
prolong gastric residence time and increase drug bioavailability. The floating tablets of
Famotidine were prepared by direct compression technique using polymers such as
hydroxypropyl methylcellulose (HPMC K4M) and polyvinyl pyrrolidone (PVP K-30). Sodium
bicarbonate was used as a gas releasing agent. The effect of various concentration of HPMC
K4M and sodium bicarbonate was studied. The formulations were optimized on the basis of
floating lag time, swelling behaviour and in-vitro drug release. The floating lag time ranged from
30 to 130 sec and swelling index value ranged from 97.71 % to 98.40 % for all the five
formulations prepared. The cumulative drug release ranged from 72.40 % to 82.40 % for all the
batches according to HPMC K4M used in the formula. The mechanism of famotidine release
from the floating tablets of F4 containing HPMC K4M (15%) and sodium bicarbonate (10%)
followed Korsemeyer peppas model with highest r
2
value of 0.997. Thus F4 batch was
considered as the optimized batch from all the five batches prepared. The results also showed
that there is increase in swelling with increase in polymer concentration.

PCEU-67

TARGETING IMMUNE SYSTEM BY NANOPARTICLES TO COMBAT CANCER

Sandeep Kumar*, Viney Lather, Deepti Pandita
Email: san_rs@ymail.com

Abstract
Immune system safeguards the host from malignancies and able to mount responses against
tumors. The cancer immunotherapy approaches are more effective and better tolerated than more
conventional treatments. Efforts to harness the immune system and to restore immune
surveillance for cancer treatment are not novel, but progress has been slow for decades.
August 22, 2014


However, recent encouraging clinical success of several anticancer immunotherapies as well as
synergistic effects of immunotherapy and chemotherapy, may herald a new era for anticancer
immunotherapy. The triumph concept of therapeutic cancer vaccines utilize the activation of the
immune system against tumor cells without collateral damage and has revolutionized the cancer
therapy, but the major hurdle for their clinical use is the unspecific delivery to the multiple sites.
Advances in protein engineering and materials science have facilitated the novel nanoscale
targeting approaches that could provide new insights to cancer immunotherapy. The efficiency of
a vaccine mainly depends on the efficient targeting of the innate immune system. Engineered
nanoparticles have shown great promise in vaccine delivery mainly due to the prolonged as well
as targeted delivery of tumor-associated antigens and immunomodulatory substances. A large
number of promising nano-sized tools have provided a boost to the field. We discuss recent
advances in the study and use of selectively immune system targeted nanoparticles in cancer
therapy.

PCEU-68

TRANSFERSOME: A NOVEL TRANSDERMAL CARRIER SYSTEM

Rajesh Kumar*, Viney Lather, Deepti Pandita
Email: rajeshkaswan4@gmail.com

Abstract
Transfersome egresses as a challenging carrier for drug delivery via transdermal route. It is
readily prepared by dissolving the lipid and surfactant in the minimum amount of acceptable
solvent and the least amount of aqueous phase. Transfersomes consist of both hydrophobic and
hydrophilic moieties, which can facilitate entrapment of wide range of therapeutics having
diverse solubility profile. The high deformability of transfersomes enhances penetration of intact
vesicles through narrow constriction. Various factors such as cost, penetration, stability etc.
make the carrier more efficient than liposomes in drug delivery. The carrier could be highly
valuable in the delivery of drugs through ophthalmic, topical, parental routes etc. Moreover
peripheral drug targeting as well as transdermal immunization can also be possible using this
system. Extensive research has been to be made on this novel drug delivery system to explore its
potential in the delivery of low as well as high molecular weight drugs e.g. corticosteroids,
anaesthetic agents, hormones, insulin, anticancer agents etc. Thus, this novel carrier has a great
potential to overcome several problems faced by the conventional techniques.

August 22, 2014


PCEU-69

GASTRIC RETENTION: AN APPROACH TO ORAL CONTROLLED DRUG
DELIVERY SYSTEM

Jyoti Dahiya*, Upma, Priti Mehndiratta, Komal Goswami, Minakshi Gupta
Shri Baba Mast Nath Institute of Pharmaceutical Sciences and Research, Asthal Bohar

Abstract
The purpose to study this approach is to develop a long -term oral controlled-release dosage form
which has been difficult mainly because of the transit of the dosage form through the
gastrointestinal (GI) tract. Gastroretentive dosage forms have many advantages and enhance the
bioavailability of drug that are characterized by a narrow absorption window. Various scientific
and technological advancements have been made in the research and development of rate-
controlled oral drug delivery systems, such as short gastric residence time (GRT) and
unpredictable gastric emptying time (GET). Several approaches have been used these days to
enhance gastric residence time. Most common of these approaches are floating drug delivery
system, high-density system, mucoadhesive system, magnetic system and swellable systems.

PCEU-70

GASTRORETENTIVE DRUG DELIVERY SYSTEM: A POTENTIAL APPROACH FOR
GASTRIC RETENTION

H. S. Chawda
1
, Y. S. Tanwar
2
, S. Pathak
1
, Deepika Deopa
1
& Garima

1. Gyan Vihar School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur, Rajasthan.
2. Department of Pharmaceutics, Bhupal Nobles College of Pharmacy, Udaipur Rajasthan.
E-mail: singhhschawda.himmat@gmail.com

Abstract:
Oral drug delivery system is the preferred route of administration of drugs because of low cost of
therapy, ease of administration and high levels of patient compliance. But the issue of poor
bioavailability of orally administered drugs is still a challenging one, though extensive
advancements in drug discovery process are made. Drugs with narrow absorption window in the
gastrointestinal tract have poor absorption. Gastric emptying of dosage forms is an extremely
variable process and ability to prolong and control the emptying time is a valuable asset for
dosage forms, which reside in the stomach for a longer period of time than conventional dosage
forms. Therefore, gastroretentive drug delivery systems (GRDDS) have been developed, which
prolong the gastric emptying time. Several techniques such as floating drug delivery system, low
density systems, raft systems, mucoadhesive systems, high density systems, superporous
hydrogels and magnetic systems, have been employed. These forms are expected to remain
buoyant on gastric content without affecting intrinsic rate of emptying. This results in prolonged
gastric retention time of floating forms which improve bioavailability of drug and also improve
August 22, 2014


clinical situations. Prolonged gastric retention not only improves the bioavailability and reduces
drug waste but also improves solubility for drugs that are less soluble in a high pH environment.
It has applications also for local drug delivery to the stomach and proximal small intestines.
Gastro retention helps to provide better availability of new products with new therapeutic
possibilities and substantial benefits for patients. Hence it can be concluded that GRDDS
promises to be a potential approach for gastric retention.
Key words: Bioavailability, Gastric Emptying, Gastro Retentive Drug Delivery Systems.

PCEU-71

TARGETED PRODRUG STRATEGY FOR OPTIMIZED DRUG DELIVERY

*Mona Piplani and Prabodh Chander Sharma
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119

Abstract
Prodrugs, the bioreversible derivatives of drug molecules, are designed to maximize the amount
of active pharmaceutical agent that reaches its site of action, through manipulation of its
physicochemical, biopharmaceutical or pharmacokinetic parameters. Conventional prodrug
design often represents a nonspecific chemical approach to masquerade detrimental drug
properties such as limited bioavailability, lack of site specificity, and chemical instability. On the
other hand, targeted prodrug design represents an innovative approach for directed and
competent drug delivery. Prodrugs can be designed to target specific enzymes or carriers by
considering enzyme-substrate specificity or carrier-substrate specificity in order to prevail over
various undesirable drug properties. This type of "targeted-prodrug" design requires considerable
knowledge of particular enzymes or carrier systems, including their molecular and functional
characteristics. Recently, advances in gene cloning and controlled gene expression techniques in
mammalian cells have allowed the elucidation of the molecular nature of enzymes and carrier
proteins and make possible more rational design of "targeted-prodrugs." 5-(Aziridine-1-yl)-2,4-
nitrobenzamide (CB 1954) for DT diaphorase enzyme, peptidyl-p-phenylenediamine-mustard for
plasmin enzyme, 5-fluorocytosine for cytosine deaminase enzyme and amygdalin for glucosidase
enzyme, etc. are few examples of enzyme targeted prodrugs. The targeted prodrug approach,
which can be pooled with gene delivery and controlled expression of enzymes and carrier
proteins, will optimistically be a promising strategy for precise as well as efficient drug delivery
and consequently would be supportive for treatment of various diseases.

August 22, 2014


PCEU-72
FORMULATION AND EVALUATION OF SI LYBUM MARI ANUM LOADED
MICROSPONGES

Archana Kaushik, Sanju Nanda, Anju Dhiman
Department of Pharmaceutical Sciences, MDU (ROHTAK)
Email: archana.kaushik92@gmail.com

Abstract
Aim: In the present research study, microsponges of S.marianum extract have been prepared and
evaluated.
Materials & Method
The sample of S.marianum seeds and eudragit RS100 were obtained as a gift from Himalaya
Drug Company, Saharanpur and Pharma Polymers (Degussa), respectively. All other polymers
and chemicals used in formulation and evaluation were obtained from Loba chemie and were of
analytical grade. Microsponges of S.marianum extract were prepared by quasi-emulsion solvent
diffusion method using ethyl cellulose and eudragit RS 100 as polymers. The external phase
comprised of PVA and Distilled Water, and internal phase comprised of S.marianum extract,
solvent, polymers. The internal phase containing S.marianum extract and eudragit polymer, ethyl
cellulose was added drop wise to the external phase with stirring at about 1500 rpm. After 10
hours of stirring, microsponges were formed which were collected on a filter paper, washed with
water and dried overnight at room temperature. The microsponges were collected, passed
through sieve followed by particle size analysis and study of flow properties.
Result: Microsponges of S.marianum extract were prepared by quasi-emulsion solvent diffusion
method. The method seems to be promising for the preparation of S.marianum extract
microsponges. Flow properties of pure drug and formulation were also good.
Conclusion:
Quasi emulsion solvent diffusion seems to be a promising method for the preparation of
S.marianum extract microsponges as it is a rapid, easy, reproducible method and has an
advantage of avoiding solvent toxicity.

PCEU-73

A REVIEW: FLOATING DRUG DELIVERY SYSTEM

Binkatesh Kumar*, Meenali Mishra and Dr. Ritu Gilhotra
GyanVihar School of Pharmacy, Suresh Gyan Vihar University, Jaipur

Abstract
The Purpose of this paper is focus on principle mechanism of floatation to achieve gastric
retention. In recent Years, scientific and technological advancement have been made in research
and development of oral drug delivery system. Oral drug delivery system is complicated by
limited gastric residence time and unpredictable gastric emptying time. To overcome these
limitations, various approaches have been proposed to increased gastric residence of drug
August 22, 2014


delivery systems, in upper part of the gastrointestinal tract includes floating drug delivery system
(FDDS), swelling or expanding systems, mucoadhesive systems, magnetic systems, modified-
shape systems, high density system and other gastric emptying devices. Floating drug delivery
system have been most commonly used to overcome these limitations and prolong gastric
retention for more than 12 hours. It enhances bioavailability, reduces drug waste, enhance
solubility of drug that less soluble in high PH environment. Floating drug delivery systems have
a bulk density less than gastric fluid and so remain buoyant in the stomach for a prolonged
period of time, releasing the drug slowly at the desired rate from the system. Dosage form
available as gastric floating system includes tablets, capsules, granules and microspheres.
Key Words: Prolong gastric residence time, buoyant system, Oral drug delivery system,

PCEU-74

NOVEL TRENDS IN PULSATILE DRUG DELIVERY TECHNOLOGY

Sarit Dhiman
*1
, Rakesh Pahwa
1

1
*
E-mail:-cdlcp.sarit@gmail.com

Abstract
Pulsatile drug delivery systems have attracted attention of large number of investigators globally
because of their multiple benefits over conventional dosage forms. Pulsatile drug delivery
systems are basically time controlled drug delivery systems that deliver the drug at specific time
as per the pathophysiological need of the disease resulting in improved patient compliance and
therapeutic efficacy. A pulsatile drug release where the drug is released rapidly after a well
defined lag-time could be advantageous for many drugs and therapies. These systems are design
according to the circadian rhythm of the body. Diseases where in these systems are promising
include asthma, peptic ulcer, cardiovascular diseases, arthritis etc. These systems can be
classified into time controlled systems wherein the drug release is controlled primarily by the
delivery system; stimuli induced pulsatile drug delivery system in which release is controlled by
the stimuli such as pH or enzymes present in the intestinal tract or enzymes present in the drug
delivery system and externally regulated systems where release is programmed by external
stimuli such as magnetism, ultrasound, electrical effect and irradiation. In conclusion, pulsatile
drug delivery system holds good promises of benefits to the patients suffering from various
chronic diseases.

August 22, 2014


PCEU-76

FORMULATION & EVALUATION OF NIMESULIDE ENTRAPPED NIOSOMES

Himmat Singh*, Ritu M Gilhotra, Meenali Mishra & Binketsh
Gyan Vihar School Of Pharmacy,
Suresh Gyan Vihar University, Jaipur, Rajasthan, India-302017
E-mail: himmat_chawda78@yahoo.com

Abstract
The delivery of drugs by vesicular drug delivery system such as niosomes or non-ionic
surfactants vesicles provides several important advantages over conventional drug therapy.
Niosomes are microscopic lamellar structures formed on the admixture of a non-ionic surfactant,
cholesterol and phosphate with subsequent hydration in aqueous media.
The aim of the study was to design suitable niosome-encapsulated drug delivery for anti-
inflammatory drugs like nimesulide and evaluate the vesicle size, encapsulation efficiency, in
vitro release and physical stability of the system. Non-ionic surfactants used were span 20, 40, 60
and cholesterol was used in different molar ratios. The niosomes prepared by lipid film hydration
method were multilamellar vesicles (MLV
S
) and niosomes prepared by ether injection technique
were unilamellar vesicles (ULV
S
) or oligolamellar vesicles. The higher entrapment efficiency
was observed with MLV
S
prepared from span 60 and cholesterol in an 80:70 molar ratio. The
invitro diffusion study suggests that higher entrapment efficiency was related with slow release
comparatively. The release pattern shown by these formulations were zero order & higuchi
diffusion controlled mechanism. The physical stability study show that niosomal preparation
stored at refrigerated temperature for 60 days show maximum drug retained for all the
formulation compare to room temperature and elevated temperature conditions. Finding of all
this investigation conclusively demonstrate prolongation of drug release at a constant and
controlled rate after niosomal encapsulation of nimesulide.
KEYWORDS: Niosome, Cholesterol, Nimesulide, Span.

PCEU-78

FORMULATION OF SUSTAINED RELEASE MATRIX TABLET
USINGHYDROPLILIC AND HYDROPHOBIC POLYMERS

Vipul Gogar*, Piyush Khandelwal, Himmat Singh, Ritu M Gilhotra
[Gyan Vihar School of Pharmacy, Suresh Gyan Vihar University, Jaipur (Raj.) 302017]

ABSTRACT
The aim of present study was designed to develop novel sustained-release (SR) matrix tablet
formulation of naproxen a non- steroidal anti inflammatory drug. The release of naproxen from
sustained release tablets based on hydrophilic matrices of hydroxyl propyl methyl cellulose
(HPMC). Hydroxy ethyl cellulose (HEC), sodium carboxy methyl cellulose (Sod. CMC) and
August 22, 2014


hydrophobic matrix polymer cetostearyl alcohol for controlled release. The fatty alcohol and
cellulose derivatives were used in the ratio of 3:1 along with usual tablets additives like lactose
& talc. The compressed matrix tablets were evaluated for various parameters like hardness,
friability, weight variation, drug content uniformity which shows the drug content was uniform
in all the formulation of the tablet prepared. IR studies indicate that the drug is compatible with
the polymers and stabilities study also performed was no appreciable difference was observed.
The in-vitro release of drug showed that tablet of HEC and Sod. CMC containing tablet (90.85
%) at the end of 8
th
hour and was found to release the drug by anomalous (non- fickian)
transport.
KEYWORDS: hydrophilic & hydrophobic matrix tablets, sustained release, naproxen,
formulation.

PCEU-79

RECENT ADVANCES AND NOVEL STRATEGIES IN DEVELOPMENT OF
NANOPARTICLE BASED THERANOSTICS

Kiran Yadav*, Dr. Sunil Kumar Yadav
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana

Abstract
When we say Theranostics, it applies to Therapeutics and Diagnostics. In recent years,
nanotechnology has been increasingly applied to the area of both therapeutics as well as
diagnostics. Nanoparticulate technology is of particular use in developing a new generation of
more effective theranostics capable of overcoming the many biological, biophysical, and
biomedical barriers that the body stages against a standard intervention. Major advances in the
use of carrier vehicles delivering pharmacologic agents, enzymes and diagnostics to sites of
disease have occurred over the past 10 years. However, despite these potential advances, only a
relatively small number of nanoparticle-based medicines have been approved for clinical use,
with numerous challenges and hurdles at different stages of development. The complexity of
nanoparticles as multi-component three dimensional constructs requires careful design and
engineering and reproducible scale-up and manufacturing process to achieve a consistent product
with the intended physicochemical characteristics, biological behaviors, and pharmacological
profiles. Overall, nanomedicines may present additional development and regulatory
considerations compared with conventional medicines, and while there is generally a lack of
regulatory standards in the examination of nanoparticle-based medicines as a unique category of
therapeutic and diagnostic agents. This work summarizes challenges likely to be encountered
during the development and approval of nanoparticle-based theranostics.

August 22, 2014


PCEU-80

COMPARATIVE BIOAVAILABILITY STUDY OF KETOPROFEN PREPARED BY
SOLID DISPERSION, BETA-CYCLODEXTRIN COPLEXATION AND SOLID
SMEDDS

Ramnaresh Uniyal, Dr.Richa Puri Ohri
Himalayan Institute of Pharmacy, Kala-amb

Abstract
Improving rate of dissolution and thus achieving effective plasma drug concentrationpossibly by
single dose administration. Thus providing a more effective control on pain associated with The
present study focuses on enhancement of dissolution of Ketoprofen (BCS II drug) and hence
bioavailability of the same by formulating Beta-cyclodextrin complex, solid dispersion and solid
SMEDDS for oral drug delivery.The ability of -cyclodextrin complex, solid dispersion and
solid SMEDDS to improve oral delivery of several therapeutic agents has been established by
various in vitro and/or in vivo methodologies. Cyclodextrins (CDs) are a family of compounds
consists of glucose monomers arranged in a donut shape ring. They are non-reducing, crystalline
cyclicoligosaccharides which proximate a truncated core generating a hydrophilic outer surface
and a lipophillic interior cavity that offers interaction withappropriately sized molecules to result
in the formation of inclusion complex.CDs help in improving the aqueous solubility of many
poorly soluble drugs, enhances the dissolution thus helps in enhancing bioavailability of drugs.
Moreover Cyclodextrin complexes improve the chemical, physical and thermal stability of drugs
(Aliet al.,2012 and Szejtliet al., 1988). Solid dispersion technology is the science ofdispersing
one or more active ingredients in aninert matrix in the solid stage in order to achieveincreased
dissolution rate, sustained release ofdrugs, altered solid state properties and enhancedrelease of
drugs from different dosage forms and improved solubility and stability. Solid dispersion is
generallyprepared with drug which is having poor aqueous solubility and hydrophilic carrier.
In solid dispersion particle size of drug is reduced or a crystalline pure drug is converted into
amorphous form andhence the solubility of drug is increased. Self Micro-emulsifying Drug
Delivery System (SMEDDS) is a novelapproach to improve water solubility and ultimate
bioavailability of drugs.Self-micro emulsifying drug delivery system is isotropic(one phase
system) mixture of oil or modified oils andsurfactants and co-surfactants, which form the fine oil
in-water micro emulsion when introduced into aqueousphase under condition of gentle agitation .

August 22, 2014


PCEU-81

FORMULATION AND I N-VI TRO EVALUATION OF ORALLY ADMINISTERED
GASTRO RETENTIVE FLOATING TABLETS OF SIMVASTATIN

Sandeep Kumar*, Peeyush Kaushik

Abstract
Gastro retentive effervescent floating tablet of simvastatin were formulated using different
grades of hydroxypropyl methylcellulose (HPMC K4M, HPMC K15M, HPMC K100M).In vitro
release, floating lag time and duration of floating of the fabricated tablets were investigated.
Gastro retentive effervescent floating tablets containing 20 mg of simvastatin were developed
using HPMC K4M, HPMC K15M, and HPMC K100M with different drug to polymer ratio,
mixture of 10% sodium bicarbonate, 2.5% citric acid anhydrous as gas generating agents,
dicalcium phosphate and lactose as fillers. Citric acid was also used as an antioxidant. Tablets
were prepared by direct compression method. The formulation was optimized to get 85 % drug
release at the end of 12 hrs and to get optimum floating lag time and buoyancy. The resulting
formulations produced robust tablets with optimum hardness, consistent uniformity in weight
and low friability. The formulation with HPMC K4M in the drug polymer ratio of 1:3 showed
85.830% drug release at the end of 12hours, maintained integrity of tablets and also has optimum
floating lag time. Tablets of all the batches floated for more than 12hrs.
The results of dissolution studies indicated that the formulation F2 (HPMC K4M 1:3 ratio) is the
most successful of the study. A decrease in release rate of the drug was observed on increasing
polymer ratio and also by increasing viscosity grades of the polymer (HPMC). The optimized
formula F2 was fitted to various kinetic models and the result showed that F2 batch followed
Zero order kinetics. The mechanism of drug release from F2 batch was anamolous non-fickian
diffusion pattern.

PCEU-82

FORMULATION AND EVALUATION OF IMMEDIATE REALEASE TABLET OF
OLMESARTAN HYDROCHLOROTHIAZIDE USING WET GRANULATION
METHOD

Nikhil Singh, Dr.Richa Puri Ohri

Abstract
The most popular solid dosage form are being tablets and capsules; one important drawback for
patients that sometime it is not disintegrates rapidly. Immediate release tablets offers less
disintegration time so rapid dissolution and great bioavailability. Olmesartan blocks;
vasoconstrictor effects of angiotensin II by selectively blocking the binding of angiotensin II to
the AT1 receptor in vascular smooth muscle(VSM). Therefore; independent of the pathways
August 22, 2014


for the angiotensin II synthesis. AT2 receptor; is found also in many tissues; but this receptor is
not known to be associated with cardiovascular homeostasis; Olmesartan more than the 12,500-
fold greater affinity for the AT1 receptor than for the AT2 receptor;
Blockade of the renin-angiotensin; system with ACE inhibitors; which inhibit the biosynthes is
of angiotensin II from angiotensin I; is a MOA of many drugs used to treatment of
hypertension; ACE inhibitors also inhibit the degradation of bradykinin; it also react catalyzed
by ACE. Becoz olmesartan medoxomil does not inhibit ACE (kininase II), it has not affected
the response to bradykinin. Blockade of the angiotensin II receptor inhibits the negative
regulatory feedback of angiotensin II on renin secretion; but the resulting improving plasma
renin activity and circulating angiotensin II levels do not overcome the effect of olmesartan on
blood pressure. Hydrochlorothiazide is a thiazide diuretic. Thiazides affect the renal tubular
mechanisms of electrolyte reabsorption, directly increasing excretion of sodium and chloride in
approximately equivalent amounts. Indirectly, the diuretic action of hydrochlorothiazide
reduces plasma volume, with consequent increases in plasma renin activity, increases in
aldosterone secretion, increases in urinary potassium loss, and decreases in serum
potassium.The renin-aldosterone link is mediated by angiotensin II, so co administration of an
angiotensin II receptor antagonist tends to reverse the potassium loss associated with these
diuretics. The mechanism of the antihypertensive effect of thiazides is not fully understood
without combine study of Olmesartan HCTZ. Immediate release tablets of Olmesartan
Hydrochlorthiazide was prepared using superdisintegrat like croscarmellose sodium.Prepared
tablets were subjected to different evaluation parameters such as hardness,friability,weight
variation,disintegration and dissolution studies and results are acceptable.

PCEU-83

FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL TABLETS
CONTAINING ANTI MIGRANT AGENT

Sanjay Kumar Pandey*, Dr. Richa Puri Ohri
Himalyan Institute of Pharmacy, Kala-amb

Abstract
The prepared tablets were evaluated for various parameters such as compatibility studies, drug
content, weight variation, hardness, thickness, friability, and swelling studies, microenvironment
pH, in vitro drug release studies, in vitro mucoadhesion strength and release rate kinetics. Further
the analysis of release mechanism was carried out by fitting the drug release data to various
kinetic equations like, zero order, first order, Krosmeyer-Peppas, Higuchi (matrix) and Hixson
Crowell equations and from the values so obtained, the best t model was arrived at. The results
obtained from FT-IR revealed that there was no chemical interaction between the drug and the
polymer used. The prepared tablets had good mucoadhesiveness. The release pattern of the
formulation was observed to be non-fickian and released drug by combination of both diffusion
and chain relaxation. Stability studies of the selected formulation was carried out to determine
the effect of formulation additives on the stability of the drug and also to determine the physical
stability of the formulation. The stability studies were carried out at 25 C/60%RH, 30 C/65%
August 22, 2014


RH and 40 C/75% RH for 90 days. There was no significant change in the physical property
and drug content during the study period. From the above the results formulation F2 was found
to be best formulation for the muccoadhesive buccal delivery of Sumatriptan Succinate that
complied with all the parameters. However, in - vivo experiments need to be carried out to know
the absorption pattern and bioavailability of drug from the muccoadhesive buccal tablets and
thus enabling us to establish in vitro - in vivo correlation.

PCEU-84

SENSITIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR
THE DETERMINATION OF TAXOL CATEGORY DRUG IN PLASMA.

Naresh Kalra
*
, Suresh Choudhary, Dr. G. Jeyabalan
Department of Pharmaceutics, Alwar Pharmacy College, Alwar. Department of Pharmaceutical
Sciences.

ABSTRACT:
The present study describes the rapid, sensitive, simple and cost-effective analytical method for
the estimation of docetaxel from rat serum. The estimation was carried out on a Lichrosphere
Lichrocart C18 column (250mm, 4mm, 5mm) using a mobile phase a mixture of Triple distilied
water and acetonitrile (50:50 v/v). The eluent was monitored on 227nm. The results have been
validated statistically and recovery studies confirmed the accuracy of proposed method. Method
was validated as per ICH guidelines.

PCEU-85

FORMULATION AND EVALUATION OF RESPERIDONE LOADED
NANOPARTICLES

Shashikant Bhatt, Dr. Richa Puri Ohri

Abstract
The objective of this study was to prepare Risperidone-loaded nanoparticles for controlled
delivery through the intravenous (i.v.) route to reduce the frequency of administration, dose and
adverse effects during the short-term management of manifestation of psychotic disorders.
Risperidone nanoparticles were prepared by Nan precipitation method and characterized for drug
content, particle size and size distribution, zeta potential, and in vitro drug-release study. In this
method, the polymer is dissolved in water-miscible solvent (acetone). Then, solution is poured
under mechanical stirring into a non solvent (usually water containing a surfactant), which leads
to precipitation of nanospheres. Reserpidone (RSP), 3-[2-[4-(6-"uoro-1,2-benzisoxazol-3-yl)-1-
piperidinyl[ethyl]-6,7,8,9-tetra-hydro-2methyl4Hpyrido[1,2a]pyrimidin-4-one, A typical
August 22, 2014


antipsychotic agent, has been approved for the treatment of psychotic disorders due to its lower
Extra-Pyramidal Side effects (EPS) compared with the conventional antipsychotics. A low-dose
RSP therapy is required to control the psychotic symptoms and long-term treatment is needed to
treat schizophrenia. All formulations were further checked for evaluation parameters. Particle
size of all formulations was found in the range of 373 to 746 nm. Zeta potential for all
formulated nanoparticles was in the range -1.70 to -29.4 which indicates they are moderately
stable. The maximum percentage yield was found to be 88.98 % for the formulation F5. The
maximum drug content was found to be 69.45 for the formulation F5 the nanoparticles exhibited
an increase in drug content with an increase in the polymer ratio, upto particular concentration
1:5. The drug entrapment efficiencies was found to be in the range of 26.43 to 65.56%.
Comparison of drug content for the formulation F1 to F7 is done. The maximum entrapment
efficiency was found to be 65.56 % for the formulation F5. The cumulative percentage release
after 12 h was found to be 28.01 to 76.68. From results it was observed that as we increase
polymer concentration, drug release from the nanoparticles decreases. So we have optimized
maximum concentration of polymer and drug in case of formulation F4 and F5 50% of the drug
get released in 12 hours. The results showed that the in vitro specifications of nanoparticles of
Risperidone are suitable for administration.

PCEU-86

COMPARATIVE STUDY AS FORMULATION AND EVALUATION OF VARIOUS
TASTE MASKING COMPLEXES OF ROXITHROMYCIN DISPERSIBLE TABLET

Uday Prakash Mishra, Dr. Richa Puri Ohri

Abstract
In the present work, Comparative study of complexation a bitter taste of Roxithromycin was
masked by complexation technique and bitterless complexes formulated into dispersible tablet.
weak cation exchange resins (INDION 214, AMBERLITE IRP64), -Cyclodextrin, and
Carbopol 934P were used in formulation of complexes with the drug. Complexation technique
was chosen for taste masking because it is simple and economic. Studies were proceeded
gradually to prepare bitter less complexes of drug and to develop dispersible tablets using the
complex with best taste masking and drug loading. Evaluation of physical properties showed that
all complexes possess good packing ability and flow properties. Dissolution study of complexes
showed that the drug was bio available from all the complexes but Drug-AMBERLITE IRP64
complex showed better release than rest of two complexes. More than 80 % of drug was released
within 30 minutes from this complex. Comparing % drug loading, physical properties and in-
vitro drug release amongst the complexes Drug-AMBERLITE IRP64 complex was selected for
formulation as this complex showed higher loading, good flow and packing ability and better
release. Also, formulated tablets were compared with marketed tablets with respect to
appearance, hardness, uniformity of weight, content uniformity and taste. The results showed
that formulated tablets completely masked the bitter taste of drug whereas marketed tablets failed
to mask the bitter taste of drug completely. Stability studies at temperature 40C for 1 month on
August 22, 2014


batch tablets confirmed that the formulated tablets possess good stability, as there was no
significant effect on physical properties, drug content and release profile of tablets. In
conclusion, results obtained in this work show that drug-resin complexes and drug-carbopol
complexes effectively masked bitter taste of Roxithromycin. formulated dispersible tablets
showed comparable release profile with that of the marketed dispersible tablets and having the
additional advantage of complete taste masking. Thus, complexation of Roxithromycin with
AMBERLITE IRP64 increases acceptability and palatability of formulated dispersible tablets.

PCEU-87

A REVIEW ON INSOLUBLE DRUG DELIVERY TECHNOLOGY

Abhinav Singh Rana
Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra
rana.kuk@rediffmail.com

Abstract
Insoluble Drug Delivery technology,which has been sucessfully,addressed the problem of water-
insoluble drug delivery.Water insoluble drug pose intricate problems in their formulation and
delivery.Poorly watersoluble drugs traditionally have been formulated for oral administration
through their micronization.Micronization increases their in-vivo dissolution rates by reducing
particle size and increasing surface area with a concomitant gain in bioavailability.New
approaches in formulating poorly soluble drugs such as the use of surface stabilized nano or
microparticles,inclusion in polymer or lipophilic matrices such as nanospheres,hydrophobic
carriers systems,self dispersable systems and molecular complexation with agents suitable for
lipophilic drugs,have demonstrated significant
improvments.Hydrophobic carrier system or self-dispersable system can be employed only for
those drugs that are significantly soluble in carrier.Similarly,a matrix-inclusion system can be
employed if the extent of drugs loading and the drug release profile within the gastrointestinal
tract are accceptable.Insoluble drug delivery technology formulations have displayed high drug
payload,low amount of free drug in the continuous phase,almost all of the drug present in the
particulate phase,no chemical change in the drug caused by the formulation process,absence of
drugs- vehicle interaction ,narrow particle size distribution with well-defined particle phase
morphology,a variety of suspensions and solid dosage form,excellent bioavailability when
raquired,and long formulations shelf-lives.

August 22, 2014


PCEU-88

APPLICATIONS OF MICROWAVE TECHNOLOGY FOR POLYMER GRAFTING

Priya Rani *, Inderbir Singh and Sandeep Arora
Department of Pharmaceutics, Chitkara College of Pharmacy, Chitkara University, Rajpura-
140401, Patiala, Punjab
E mail: pnangru@gmail.com

Abstract
The use of microwave irradiation has become a common heat source in organic chemistry. The
microwave irradiation is also increasingly studied for polymerization reactions. Microwave
technique offer a number of advantages like rapid reactions, high purity of products, less side-
products, improved yields, simplified and improved synthetic procedure, wider usable range of
temperature, higher energy efficiency, sophisticated measurement and safety technology,
modular systems enable changing from mg to kg scale. However certain disadvantages like heat
force control is difficult, water evaporation and closed container is dangerous because it could be
burst, are also associated with this technique. The present review discusses the technique and
principle involved in microwave assisted technique for grafting of the polymers. Various
pharmaceutical applications like release retardant, tablet superdisintegrant, flocculant,
mucoadhesion of the microwave assisted grafted polymers have been discussed. Microwave
assisted extraction of various compounds from materials of natural origin is also gaining
importance among researchers. In conclusion, microwave assisted grafting of polymers for
specific application is a useful technique modifying important pharmaceutical properties of the
polymers.

PCEU-89

SUSTAINED RELEASE MATRIX TECHNOLOGY AND RECENT ADVANCE IN
MATRIX DRUG DELIVERY SYSTEM

Surendra Bhambu*, Dr. Shiv Kumar Garg
Maharishi Arvind College of Pharmacy, Ambabari, Jaipur, Rajasthan, India
E.Mail: bhambusurendra803@gmail.com
Abstract
The oral route is the most popular route used for administration of drugs, which is due in part to
the ease of administration and to the fact that gastrointestinal physiology offers more flexibility
in dosage form design than most other routes. Sustained release, sustained action, prolonged
action and extended action are the terms used to identify drug delivery system that are designed
to achieve a prolog therapeutic effect by continuously releasing medication over an extended
period of time after administration of a single dose. sustained release (matrix) drug delivery over
conventional dosage forms like improved patient compliance due to less frequent drug
August 22, 2014


administration, reduction of fluctuation in steady-state drug levels, maximum utilization of the
drug, increased safety margin of potent drug, reduction in healthcare costs through improved
therapy and shorter treatment period. The basic goal of sustained release is provide promising
way to decrease the side effect of drug by preventing the fluctuation of the therapeutic
concentration of the drug in the body and increase patient compliance by reducing frequency of
dose.

Keywords:- Extended release, Sustained-release, Matrix tablet.

PCEU-90

CONTROLLED RELEASE ION EXCHANGE RESIN DRUG DELIVERY SYSTEM
AND RECENT DEVELOPMENT IN ION EXCHANGE RESIN DELIVERY SYSTEM

Bhaskar Barsar
*
, Dr. Shiv Kumar Garg
Maharishi Arvind College Of Pharmacy, Ambabari, Jaipur,Rajasthan, INDIA
E-Mail: bhasu.barsar@gmail.com, gargshiv81@yahoo.co.in

Abstract
The oral route is the most popular route used for administration of drug Controlled drug delivery
systems aim to maintain plasma concentration of drugs within the therapeutic window for a
longer period of time, thereby to ensure sustained therapeutic action and for that reason an
increasing interest in their development exist. Ion exchange resins are cross-linked water
insoluble polymer-carrying, ionizable functional groups. IER have received considerable
attention from pharmaceutical scientists because of their versatile properties as drug delivery
vehicles. Research over the last few years has revealed that IER are equally suitable for drug
delivery technologies, including controlled release, transdermal, nasal, topical and taste masking.
The use of IER has occupied an important place in the development of controlled- or sustained-
release systems because of their better drug-retaining properties and prevention of dose dumping.
Synthetic ion exchange resins have been used in pharmacy and medicine for taste masking or
controlled release of drug. Drug resin complexation converts drug to amorphous form leading to
improved drug dissolution. Several studies have reported the use of IER for drug delivery at the
desired site of action. Sulfonated and carboxylic resins with a polystyrene backbone are most
widely used in clinical medicine.
Key words Ion exchange resins, taste masking, resin drug complex, controlled release.

August 22, 2014


PCEU-91

DEVELOPMENT AND CHARACTERIZATION OF ANTIFUNGAL GEL OF
CLOTRIMAZOLE

Shreeram Bangarwa
Maharishi Arvind college of Pharmacy, Ambabari, jaipur

Abstract:
Fungal infection of skin is now-a-days one of the common dermatological problem. The
physicians have a wide choice for treatment from solid dosage to semisolid dosage form and to
liquid dosage formulation. Among the topical formulation clear transparent gels have widely
accepted in both cosmetics and pharmaceuticals. Clotrimazole can be formulated by cold
mechanical method by using Mucilage, Sodium alginate(natural polymer), Hydroxy propyl
methyl cellulose, Hydroxy propyl cellulose (Synthic polymer gelling agent), Dimethyl Sulfoxide
(Permeation enhancer), Triethanolamine (neutraling agent), Methyl or Water methanol
mixture(Solvent) etc.

PCEU-92

MATRIX TABLETS: AN APPROACH TOWARDS ORAL EXTENDED RELEASE
DRUG DELIVERY

Sohan Lal*, Dr. Shiv Kr.Garg, Ajay Pareek
Maharishi Arvind College of Pharmacy, Amba bari, Jaipur, Rajasthan, India
E-mail: sohan2182@gmail.com
Abstract
If one were to imagine the ideal drug delivery system, two prerequisites would be required.
First, it would be a single dose for the duration of treatment, whether it is for days or weeks,
as with infection, or for the lifetime of the patient, as in hypertension or diabetes. Second, it
should deliver the active entity directly to the site of action, thereby minimizing or
eliminating side effects. This may necessitate delivery to specific receptors or to
localization to cells or to specific areas of the body.
In the past decade great interest got generated on replacing conventional administration
of drugs by delivery systems which would release effective quantities from a protected
supply at a controlled rate over a long period of time. Ideally a drug to provide desired
therapeutic action should arrive rapidly at the site of action (receptor) in optimum
concentration, remain there for desired time, spare other sites and get removed from the site.
One of the interesting results of pharmaceutical research is the fact that absorption rate of a
drug can be decreased by reducing its rate of release from the dosage form. The products so
formulated are designated as sustained action, sustained release, delayed action, prolonged
action, depot, repository, retarded release and timed release medication.
August 22, 2014


Oral ingestion has been the most convenient and commonly employed route of drug delivery.
Indeed, for sustained-release systems, the oral route of administration has by far received the
most attention with respect to research on physiological and drug constraints as well as
design and testing of products. This is because there is more flexibility in dosage form
design for the oral route than there is for the parenteral route
Keywords: Extended release, Therapeutic concentration, Patient convenience and compliance.
Controlled release; Hydrophilic matrix; hydrophobic matrix; Matrix tablets;

PCEU-93

MITOCHONDRIA TARGETTED ANTIOXIDANTS

Shubhangi Chauhan*, Kuldeep Vyas, Dinesh Kumawat
Email: angelshubh@gmail.com

Abstract
Mitochondria are central to oxidative phosphorylation and much of metabolism, and are also
involved in many aspects of cell death. Consequently, mitochondrial dysfunction contributes to a
wide range of human pathologies. In many of these, excessive oxidative damage is a major factor
because the mitochondrial respiratory chain is a significant source of the damaging reactive
oxygen species superoxide and hydrogen peroxide. However, despite the clinical importance of
mitochondrial oxidative damage, antioxidants have been of limited therapeutic success. This may
be because the antioxidants are not selectively taken up by mitochondria, but instead are
dispersed throughout the body. To address this unmet need, a series of mitochondria-targeted
antioxidants have been developed over the past few years that are selectively concentrated within
mitochondria in vivo. The accumulation of an antioxidant at the site where it is needed most has
been shown to improve the outcome in a large number of animal models of diseases that involve
mitochondrial oxidative damage. Mitochondria-targeted antioxidants have also been developed
as pharmaceuticals and have been shown to be safe and effective in human clinical trial phase II
studies. Therefore the mitochondria-targeted antioxidants are a new class of pharmaceuticals that
can be used in a wide range of human pathologies for which current therapies are of limited
efficacy.

PCEU-94

SUSTAINED RELEASE MATRIX TECHNOLOGY AND RECENT ADVANCE IN
MATRIX DRUG DELIVERY SYSTEM

Deepu Puri*, Dr. Shiv Kr.Garg, Ajay Pareek
Maharishi Arvind College of Pharmacy, Amba bari, Jaipur, Rajasthan, INDIA
E-mail: goswamideepupuri@gmail.com
August 22, 2014


Abstract
Of all drug delivery systems, oral drug delivery remains the most preferred option for
administration for various drugs. Through this route tablet, capsule, suspensions, solutions,
syrups are administered. As very few drugs are coming out of research and development and
already existing drugs are suffering the problem of resistance due to their irrational use. Hence,
change in the operation is a suitable and optimized way to make the some drug more effective by
slight alteration in the drug delivery. Wide variety of polymers is available for retarding the
release rate of drug hence sustains the action of drug. Sustained Release is also providing
promising way to decrease the side effect of drug by preventing the fluctuation of the therapeutic
concentration of the drug in the body. Oral sustained release (SR) or controlled release(CR)
products provide an advantage over conventional dosage forms by optimizing bio pharmaceutics,
pharmacokinetic and pharmacodynamic properties of drugs in such a way that it reduces dosing
frequency to an extent that once daily dose is sufficient for therapeutic management through
uniform plasma concentration providing maximum utility of drug with reduction in local and
systemic side effects and cure or control condition in shortest possible time by smallest quantity
of drug to assure greater patient compliance. This review describes the various types of sustained
release or controlled release dosage forms, along with these factors influencing the design and
performance of sustained/controlled release products are also discussed.
Keywords: Absorption window, Controlled release, Half-life, Sustained release

PCEU-95

SYNTHESIS AND CHARACTERIZATION OF SILVER AND GUAR GUM
NANOPARTICLES

Deepika Aggarwal*, Swati Malik
Himalayan Institute of Pharmacy,Kala-amb

Abstract
Nanoparticles are drug carriers for targeted delivery, with a size range about 10 and 1000 nm
which are made of non-biodegradable and biodegradable polymers. They diffuse in the body
very well but can be recognized by the human body as foreigner intruders, so easily opsonized
and removed from the blood circulation. Nanoparticulate delivery systems have some advantages
including to control drug release profiles, prolonging the presence of drugs in blood circulation,
and to target drugs to a specific site. Depending upon the method of preparation, nanoparticles,
nanospheres or nanocapsules can be obtained. Nanocapsules are systems in which the drug is
confined to a cavity surrounded by a unique polymer membrane, while nanospheres are matrix
systems in which the drug is physically and uniformly dispersed. Silver nanoparticles have
distinctive physico-chemical properties, including a high electrical and thermal conductivity,
surface-enhanced Raman scattering, chemical stability, catalytic activity and non linear optical
behavior. Silver nanoparticles also exhibit broad spectrum bactericidal and fungicidal activity.
Scanning and transmission electron microscopy (SEM and TEM) were used to study the biocidal
action of this nanoscale material. The silver nanoparticles have been demonstrated as an effective
August 22, 2014


biocide against broad-spectrum bacteria including both Gram-negative and Gram-positive
bacteria, in which there are many highly pathogenic bacterial strains. Silver nanoparticles using
guar gum as native gum which can be used as powerful disinfectant for control and prevention of
microbial infections. Guar gum is natural and biodegradable polymer, which is non-toxic vehicle
as well as stabilize the nanoparticles and thus these silver- based nanocomposites will be
environmentally.

PCEU-96

STUDY OF DISSOLUTION RATE PROFILE OF MODIFIED RELEASE PELLETS
CONTAINING VENLAFAXINE HYDROCHLORIDE

Sudhir Kumar*, Dr. Richa Puri Ohri

Abstract
In past decade great interest got generated on replacing conventional administration of drugs by
delivery system which would release effective quantities form a protected supply at a controlled
rate over a long period of time. Immediate release dosage form results repaid rise in plasma
concentration within a short period after administration. Subsequently due to metabolism and
elimination the plasma drug concentration falls below the therapeutic level.
Dissolution is defined as the process by which solid substance enters solvent to yield a solution.
Simply, dissolution is a mass transfer from a solid surface to liquid phase. It clearly stated that
dissolution is dynamic property. Dissolution testing has almost had a century of development. It
expanded over years beyond the ordinary tablets and capsules, first to Extended-release and
delayedrelease (enteric-coated) articles, then to transdermals, multivitamin and minerals
products, and to class monographs for non-prescription drug combinations. It was in the year
1897 that Noyes and Whitney published a paper on Rate of solution of solid substance in their
own solution which gives the first known reference dissolution testing.

PCEU-97

CHRONOPHARMACEUTICS - A NOVEL APPROACH FOR DRUG DELIVERY

Priyanka Kriplani
Department of pharmaceutical sciences, Guru Gobind Singh College of Pharmacy
E-mail- priyanka15n@gmail.com

Abstract
All functions in human body are highly organized in time as biological rhythms of diverse periods, both
in health and in disease. This represents a challenge for those involved in the development of drug-
delivery systems to make possible the treatment of illness according to these physiological biological
August 22, 2014


rhythms as a means of improving therapeutic outcomes. Chronopharmaceutics is an emerging discipline
combining the traditional goal of pharmaceutics (sciences of drug delivery systems) with recent
knowledge in different disciplines derived from advances in chronobiology. Basically, the advances in
chronobiology and related disciplines have led to a plethora of data demonstrating the extent of generality
and precision of biological rhythms that may be used intelligently for the development of novel drug
delivery systems, as well as drugs, to optimize their efficacy and safety. However, pharmaceutical
companies are experiencing obstacles in discovering new medications that represent significant advances
in the treatment of disease.

August 22, 2014


PHARMACOLOGY
ABSTRACTS

August 22, 2014


PCL-1

ISOLATION, CHARACTERIZATION AND -AMYLASE ACTIVITY OF QUERCITIN
FROM PI NUS ROXBURGHI I

Dhirender Kaushik, Pawan Kaushik*, Gulshan Singh
1
, Sukhbir Lal Khokra, A.C Rana,
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra 136119.
1
Department of Chemistry, Kurukshetra University, Kurukshetra 136119.

Abstract
Pinus roxburghii Sarg. is a traditional herb used in the treatment of diabetes mellitus
ethnopharmacology in India and Africa. In this study, we investigated the antidiabetic activity
using -amylase inhibitory assay on extracts and isolated compound from the bark of Pinus
roxburghii Sarg. The isolated compound demonstrated the -amylase inhibitory from Pinus
roxburghii Sarg. bark. This is reported, from this plant, for the first time. The ethanol extract, n-
butanol fraction and the isolated compound exhibited significant enzyme inhibitory activity
against -amylase. Our study revealed, for the first time, the isolation and -amylase inhibitory
activity of quercetin from Pinus roxburghii Sarg. bark (PRB).

PCL-2

ANTIMICROBIAL POTENTIAL OF VARIOUS LEAF EXTRACTS OF BLUMEA
SPECIES

Amanjot Kaur and Ritu Mahajan*
Department of Biotechnology, Kurukshetra University, Kurukshetra (Haryana)
*Correspondence: ritupanipat@rediffmail.com

Abstract
A need is always felt in the pharma sector to search for new antimicrobial compounds due to
increased cases of development of resistance by microorganisms to the currently used antibiotics.
Scientific evaluation of the antimicrobial activity of widely distributed plants against various
types of microbes still remains an area of intensive investigation. The antimicrobial potential of
various extracts of Leaves of Blumea plant was evaluated against bacterial and fungal species.
For assaying antimicrobial activity, the agar well diffusion method of Perez et al. and Rojas et al.
was used with minor modifications. Ampicillin was used as positive control. Similarly, a
negative control was also tested using the different solvents. The test was carried out in
triplicates. The plates were incubated at 32.5 2.5
0
C for 24 48 hrs. The antimicrobial activity
in terms of percentage relative inhibition zone diameter was measured. The MIC value was
determined with various extracts by agar well diffusion technique using serial dilutions. The least
concentration of each extract showing a clear zone of inhibition was taken as the MIC. Results of
our studies indicate the fair antimicrobial potential of Blumea against Bacillus subtilis,
Staphylococcus aureus, Serratia marcescens, Candida albicans. Percentage of Relative
August 22, 2014


Inhibition Zone Diameter for Bacillus subtilis, Staphylococcus aureus, Serratia marcescens,
Candida albicans were 50,75,50 and 50 % respectively with MIC value of 5mg/ml.

PCL-3

DRUG-RESISTANT MALARIA IN SOUTH ASIAN COUNTRIES: A REVIEW OF
EVIDENCE AND FUTURE PROSPECTS OF NANOMEDICINE BASED STRATEGIES
FOR PROPHYLAXIS AND TREATMENT

Mohit*, Bindu garg, Arvind sharma, Sandeep arora
University, Chandigarh-Patiala National Highway (NH-64) Punjab 140401, India,
Email mohit.bhagrath@gmail.com

Abstract
International experts raised the alarm over the spread of drug-resistant malaria in several
Southeast Asian countries, saying it endangers major global gains in fighting the mosquito-borne
disease that kills more than 600,000 people each year. The availability of therapies using the
drug artemisinin has helped cut global malaria deaths by a quarter in the past decade. But over
the same period, resistance to the drug emerged on Thailands borders with Myanmar and
Cambodia and has spread tremendously. It has been detected in southern Vietnam and probably
exists in southern Laos. . Once it reaches a higher level of resistance where the drugs dont work,
we are technically stuffed, Scientists have been working for decades to develop a malaria
vaccine, but none is yet available. To counteract this trend, research has been done in
nanotechnology and nanomedicine, for the development of new biocompatible systems capable
of incorporating drugs, lowering the resistance progress, contributing for diagnosis, control and
treatment of malaria by target delivery. In this review, we discussed the main problems
associated with the spread of malaria and the most recent developments in nanomedicine for
anti-malarial drug delivery.

PCL-4

SODIUM PHENYLBUTYRATE, A HISTONE DEACETYLASE INHIBITOR,
PROTECTS AGAINST CHRONIC ETHANOL-INDUCED COGNITIVE
DYSFUNCTION AND ALTERATION IN HIPPOCAMPAL BNDF EXPRESSION
LEVEL

Ashok Jangra
1
, Satendra Singh Gurjar
2
, Chandra Shekhar Sriram
1
, Md. Iftikar Hussain
3
, Probodh
Borah
3
, Mangala Lahkar
1, 4

1
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education
and Research, Guwahati, Assam-781032
2
Department of Biotechnology, National Institute of Pharmaceutical Education and Research,
Guwahati, Assam-781032
3
State Biotech Hub, College of Veterinary Science, Assam-781022
August 22, 2014


4
Department of Pharmacology, Gauhati Medical College, Guwahati, Assam-781032

Abstract
Chronic alcohol consumption is associated with an increased risk of alcoholism, malnutrition,
chronic pancreatitis, alcoholic liver disease and cancer. Chronic alcohol abuse is well known to
cause neurodegeneration in both human and rodent brains, leading to severe cognitive
impairment.Histone deacetylation is an epigenetic mechanism which leads totranscriptional
repression of various genes. Our studyattempted todemonstrate that histone deacetylation plays a
crucial role in ethanol-induced cognitive impairment and changes in hippocampal BDNF
level.Ethanol was made continuously available in 0.2% saccharin for 8 weeks to male Sprague
Dawley rats. The histone deacetylase inhibitor, sodium phenylbutyrate was given
intraperitoneally at 40 mg/kg for last 14 days of the study. Cognitive impairment induced by
chronic alcoholism was assessed by Morris water maze test on 56
th
day.Animals were sacrificed
on the same day by decapitation, and hippocampus was isolated quickly from the brain for
estimation of BDNF protein and BDNF mRNA level. BDNF gene expression level in
hippocampus was measured by Quantitative Real-time PCR. It was observed that chronic
alcoholism resulted in significant cognitive impairment assessed by Morris water maze test.
Furthermore, BDNF protein as well as its mRNA level in hippocampus was found to be reduced
by chronic alcoholism. It was found that histone deacetylase inhibition by Sodium
phenylbutyrate (40 mg/kg) significantly improved the cognitive functionand up-regulate
thehippocampal BDNF level as compared to alcoholic group. Theresults emphasize the
beneficial effect of targeting histone deacetylase by sodium phenylbutyrate against chronic
alcoholism-induced cognitive impairment.

PCL-6

INDIAN MUSTARD (BRASSICA J UNCEA) REMNANT AFTER OIL EXTRACTION
PROTECT BIOMOLECULES AGAINST I NVI TRO OXIDATION

Anita Dua
1
*, Gaurav Garg
2
, Ritu Mahajan
3

1
Biochemistry Department, University College, Kurukshetra University, Kurukshetra
2
Department of Biotechnology, Maharishi Markandeshwar University, Mullana
3
Department of Biotechnology, Kurukshetra University, Kurukshetra
Email: anitadua2012@gmail.com

Abstract
Indian mustard seeds were defatted by distillation with hexane and the residue extracted with
methanol was analyzed for potential antioxidants; ascorbate, riboflavin and polyphenols. Gallic
acid (129.796g), caffeic acid (753.455g), quercetin (478.352g) and kaempferol (48.060
g)/g dry seeds were identified by HPLC analysis of the extract. DPPH free radical scavenging
activity and protection of lipids, proteins and DNA against metal induced oxidation was
examined. Defatted mustard seed residue had excellent free radical scavenging activity and
protects biomolecules with IC
50
value 2.0- 2.25 mg dry seed weight. Significant content of
polyphenols in methanol extract of defatted mustard seeds including gallic acid, caffeic acid,
August 22, 2014


quercetin and kaempferol accounts for high antioxidant activity. We are the first to report the
detailed analysis of antioxidant composition and protection of biomolecules against oxidative
damage by methanol extract of mustard seed remnant after oil extraction.

PCL-7

ANTI-ULCER ACTIVITY OF DEGLYCYRRHIZINIZED LIQUORICE

Kartik Sharma*, Vibhu Kumar, Kanav Midha, Vandana Saini and Anju Goyal
Chitkara College of Pharmacy, Chitkara University, Rajpura
E-mail: kkartik431@gmail.com

Abstract
Many attempts have been made to improve the treatment of gastriculcers for the previous years.
In the past years evidence has accumulatedthat liquorice and related compounds accelerate
therate of healing of gastric ulcers.Liquorice is a plant of ancient origin that occurs as roots and
stolons of Glycyrrhiza glabra belonging to family Leguminosae. Liquorice extract and its
principal component, glycyrrhizin has been used in food and herbal, traditional medicines for
thousands of years. It is also called as sweet root as it contains a compound that is about 50 times
sweeter than sugar.. Liquorice is sometimes suggested for cough, asthma, and other breathing
problems while the topical preparations are used for eczema and other skin problems. On
removing glycyrrhizin from liquorice, deglycyrrhizined liquorice is obtained. Deglycyrrhizinated
liquorice, (DGL) is a herbal supplement that is used in the treatment
of gastric and duodenal ulcers. Biochemical studies indicate that glycyrrhizinate inhibits 11-
hydrox ysteroid dehydrogenase, the enzyme responsible for inactivating cortisol. As a result, the
continuous, high level exposure to glycyrrhizin compounds can produce hypermineralocorticoid-
like effects in both animals and humans.

PCL-8

POSSIBLE PROTECTIVE EFFECT OF PPAR-ALPHA AGONIST AND CCBS
AGAINST 3-NP INDUCED HD

Parul*
1
, Prabhsharan Kaur
1
, Puneet Kumar
2
, Vijender Kumar
1
, Arun Kaura
1
,
Sandeep Kumar Goyal
1

1
University Institute of Pharmaceutical Sciences and Research, Baba Farid University of Health
Sciences, Faridkot- 151 203
2
ISF College of Pharmacy, Moga

Abstract:
The present study has been designed to investigate the the protective effect of PPAR-alpha
agonist and CCBs against 3-nitropropionic acid-induced Huntington's disease-like symptoms in
August 22, 2014


rats. The present experimental protocol design includes systemic 3-nitropropionic acid (10 mg/kg
i.p) treatment for 14 days. PPAR-alpha agonist and CCBs were given orally, once a day, 1 h
before 3-nitropropionic acid treatment for 14 days. Body weight and behavioral parameters
(locomotor and rotarod activity) were assessed on 1st, 5th, 10th and 15th day post-3-
nitropropionic acid administration. Malondialdehyde, nitrite concentration, superoxide dismutase
and catalase levels were measured on the 15th day in the striatum. Systemic 3-nitropropionic
acid treatment significantly reduced body weight, locomotor activity and oxidative defense. The
mitochondrial enzyme activities were also significantly impaired in the examined brain regions
in 3-nitropropionic acid-treated animals. PPAR-alpha agonist and CCBs treatments significantly
attenuated the impairment in behavioral and biochemical parameters as compared to the 3-
nitropropionic acid-treated group. The results of the present study suggest that the combination
of PPAR-alpha agonist and CCBs produced a synergistic protective effect against 3-NP-induced
behavioral and biochemical alterations in rats.

PCL-9

EFFECT OF GINSENG AGAINST HALOPERIDOL INDUCED OROFACIAL
DYSKINESIA

Rupinder Kaur*
1
, Narmeen Kaur
1
, Puneet Kumar
2
, Vijender Kumar
1
, Arun Kaura
1
,
Sandeep Kumar Goyal
1

1
2
ISF College of Pharmacy, Moga

Abstract:
Chronic treatment with neuroleptic agents produce the development of abnormal orofacial
movements called vacuous chewing movements (VCMs) in rats. Vacuous chewing movements
in rodents are widely accepted as one of the animal models of tardive dyskinesia. Oxidative
stress & lipid peroxidation products are implicated in the pathophysiology of many neurological
disorders including tardive dyskinesia. In the present study chronic haloperidol for 21 days
treatment induced vacuous chewing movements and tongue protrusions in rats. Administration of
ginseng, dose dependently reduced haloperidol-induced vacuous chewing movements and tongue
protrusions. Biochemical analysis revealed that chronic haloperidol treatment induces lipid
peroxidation and decreases the glutathione (GSH) levels in the forebrains of rats. The antioxidant
defense enzymes, superoxide dismutase (SOD) and catalase were also decreased due to chronic
haloperidol treatment. Administration of ginseng significantly reduced the lipid peroxidation and
restored the decreased glutathione levels in these rats. Further, ginseng also reversed the
haloperidol-induced decrease in forebrain SOD and catalase levels in rats. The major findings of
the present study suggested that oxidative stress plays a significant role in neuroleptic-induced
orofacial dyskinesia and ginseng administration reverses these behavioral and biochemical
changes. So, the study suggests that ginseng may be a useful drug in neuroleptic-induced
orofacial dyskinesia.

August 22, 2014


PCL-10

EFFECT OF DPP-IV INHIBITOR ON EXPERIMENTAL ENDOTHELIAL
DYSFUNCTION

Uma Jyoti*, Samridhi Sharma, Sunil Kumar Kansal, Vijender Kumar, Arun Kaura,
Sandeep Kumar Goyal
University Institute of Pharmaceutical Sciences and Research,Baba Farid University of Health

Abstract:
Vascular endothelial dysfunction (VED) is defined as imbalance between vasoconstriction and
vasodilatory substances, thrombosis and thrombolysis, growth promotion and growth regulation.
The present study has been designed to investigate the effect of linagliptin, a dipeptidyl peptidase
IV (DPP-IV) inhibitor in sodium arsenite-induced vascular endothelial dysfunction (VED) in
rats. The rats were administered sodium arsenite (1.5 mg/kg/day, i.p., 2 weeks) to induce VED.
The development of VED was assessed by employing isolated aortic ring preparation and
estimating serum nitrite/nitrate concentration. Further, the integrity of the aortic endothelium was
assessed histologically using haematoxylin-eosin staining. Moreover, the oxidative stress was
assessed by estimating serum thiobarbituric acid reactive substances. The administration of
sodium arsenite produced VED by impairing acetylcholine-induced endothelium dependent
relaxations, diminishing the integrity of vascular endothelium and decreasing the serum
nitrite/nitrate concentration. In addition, sodium arsenite was noted to produce oxidative stress as
it increased serum thiobarbituric acid reactive substances. Treatment with linagliptin
significantly prevented sodium arsenite-induced VED by enhancing acetylcholine-induced
endothelium dependent relaxation, improving the integrity of vascular endothelium, increasing
the nitrite/nitrate concentration and decreasing the oxidative stress. However, the vascular
protective effect of linagliptin was markedly abolished by co-administration of nitric oxide
synthase inhibitor, N-Omega-Nitro-L-Arginine Methyl Ester (L-NAME) (25 mg/kg/day, i.p.).
Thus, it may be concluded that linagliptin reduces oxidative stress, activates eNOS and enhances
the generation of nitric oxide to prevent sodium arsenite-induced VED in rats.

PCL-11

EFFECT OF PROGESTERONE AGAINST EXPERIMENTAL PARKINSONS
DISEASE

Gurmeet Kaur*
1
, Rajesh Kumar
1
, Puneet Kumar
2
, Rahul Deshmukh
2
, Vijender Kumar
1
, Arun
Kaura
1
, Sandeep Kumar Goyal
1

1
2
ISF College of Pharmacy, Moga.

August 22, 2014


Abstract:
The present study has been designed to evaluate the neuroprotective effect of the progesterone
against rotenone-induced Parkinsonism in rats. Parkinson's disease (PD) is a neurodegenerative
disorder, for which no effective treatment approaches are available. Rotenone, a potent specific
inhibitor of mitochondrial complex-1, appears to produce the behavioral features of Parkinson's
disease in rats by inducing oxidative stress and thereby neurotoxicity. Rats were treated with
rotenone 1.5 mg/kg (s.c.) for 4 weeks. Behavioural assessment was done using open field test,
narrow-beam walk and rotarod apparatus. Biochemical estimations of LPO, catalase, SOD,
Nitrite, reduced glutathione and total protein content was done for evaluation of PD. Both
behavioural and biochemical estimations confirmed the establishment of PD symptoms. Data
showed impaired motor function, significant increase in catalepsy, decrease in locomotor activity
and decrease in muscle activity. Lipid peroxidation was found to increase significantly in
rotenone treated animals when compared with co-treatment of progesterone. Co-treatment with
progesterone significantly attenuated the extent of motor dysfunction. Thus, the present study
suggests that progesterone co-treatment attenuates rotenone induced motor dysfunction by its
antioxidant action.

PCL-12

EFFECT OF ETHYL ACETATE STEM BARKFRACTION OF BETULAALNOI DES AGAINST
MPTP- INDUCED EXPERIMENTAL PARKINSON'S DISEASE IN RATS

ArshvirKaur*, Vishavdeep Sharma, Rahul DeshmukhDepartment of Pharmacology, I.S.F
College of Pharmacy, Ferozepur Road, GhalKalan, Moga 142001, Punjab, India,
E-mail-arshvirbedi@yahoo.com

Abstract:
Parkinson disease (PD) is a hypokinetic movement disorder characterized by degeneration
ofdopaminergic neurons in nigral region of the brain. Impairment in striatal cyclic nucleotide
signalinghas been reported to occur in experimental as well as human PD. Various
Phosphodiesterase (PDE's)inhibitor have been reported to enhance striatal cyclic nucleotide level
& restore motor functions inexperimental PD. Recently, ethanolic extract of stem bark of Betula
alnoides has been reported toinhibit PDE's with IC50 of 3.79g/ml. In the present study we have
investigated the effect of stem barkethyl acetate fraction of B. alnoides (EASBA) against 1-
methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) induced behavioral motor deficit &
biochemical alterations in rats. MPTP was repeatedly administered intranigrally (bilaterally) at
an interval of three days (day 0, 4 and 8) to produce stablemotor deficit. Animals were treated
with EASBA at different doses (30 and 60 mg/kg p.o.) from day 1-15. Motor deficit in MPTP
treated rats was assessed by set of behavioral parameters narrow beamwalk, foot slip counts, grip
strength, rota rod & open field tests in rats. Biochemical stress of oxidative(MDA, Nitrite &
GSH) and inflammatory (TNF- & IL-) mediators in striatal brain tissue were assessedby
measuring the levels in striatal brain homogenate. Chronic administration of EASBA
significantlyand dose dependently improved motor behavior, attenuated oxidative-inflammatory
August 22, 2014


stress in MPTPtreated rats. Results from current study have supported neuroprotective effect as
outcome of PDEinhibition by EASBA in MPTP assaulted rats.

PCL-13

HEPATOPROTECTIVE EFFECT HERBAL DRUGS AGAINST HIGH FAT DIET
AND ALCOHOL INDUCE HEPATOTOXICITY IN RATS: POSSIBLE SYNERGISTIC
EFFECT

Ganesh Singh Bhakuni*, Onkar Bedi,Vinod Gauttam,Krishna Reddy V. Bijjem,Puneet Kumar
Department of Pharmacology, I.S.F College of Pharmacy, Moga-142001, Punjab, India
Email-bhakuniganesh17@yahoo.com

Abstract:
There are various liver disorders including both non-alcoholic and alcoholic fatty liver disorder
which might be due to changing diet styles, leading to apoptosis, necrosis, ischemia and oxidative
stress.The present study was designed to elucidate the hepatoprotective potential of three herbs and
their possible HO-1 modulation against HFD and alcohol induced hepatotoxicity. The
hepatoprotective activity of Phyllanthusniruri (PN), Andrographispaniculata (AP) and Piper longum
(PL) of different combinations was evaluated both in-vivo and in-vitro against40% Alcohol
2ml/100g with HFD and ethanol (100mM) in Wistarrats and HepG-2 cell lines respectively. The
extracts combinations were first studied in invitro on HepG-2 and then studied on rats. The different
combinations of three extracts were evaluated against liver toxicity induce by 40% Alcohol
2ml/100g with HFD in Wistar rats (21 days). Oxidative stress parameters, liver enzymes, Lipid
profile and histopathological and mechanistic study were assessed in liver homogenate. The body
weight and urine analysis was done on 7
th
, 14
th
, 21
st
day. The treatment with different combinations
of herbs (AP: PN: PL, 166:166:166 mg/kg, 200:200:100mg/kg,
300:100:100mg/kg,100:300:100mg/kg) proved to be hepatoprotective further combination of equal
parts of all herbs ratio showed significant effect as compared to other ratios. There was significant
reduction in LPO, nitrite, increase in antioxidant level and significantly improved in both lipid
profile and liver enzymes level. The mechanism of hepatoprotective effect of three herbal is
proposed to be by normalize ROSs and stimulating HO-1 level. The conclusion of the present study
suggests that a HO-1 and antioxidant is the mechanism involved in the protective effect of best
reported herbal combination (AP: PN: PL, 1:1:1,166:166:166 mg/kg) against HFD and alcohol
induce hepatotoxicity.

August 22, 2014


PCL-14

ROLE OF HEMEOXYGENASE-1/GLYCOGEN SYNTHASE KINASE-3 PATHWAY
IN 3-NITROPROPIONIC ACID INDUCED NEUROTOXICITY IN RATS

NavneetKaur*, Aamir Khan, Krishna Reddy V. Bijjem, Atish Prakash, Puneet Kumar
Department of Pharmacology, I.S.F College of Pharmacy, Ferozepur Road, GhalKalan, Moga-
142001, Punjab, India
E-mail: nav_neet@hotmail.co.uk

Abstract:
HD is a neurodegenerative disorder, characterized by degenerative process with behavioral and
cognitive deficits. Recently GSK-3 and HO-1 are implicated in the pathophysiology of
neurodegeneration. The present study was designed to explore the possible interaction between HO-
1 and GSK-3 pathway in 3-NP induced neurotoxicity in rats. Systemic administration of 3-NP (10
mg/kg, i.p) treatment for 14 days. Hemin (10 and 30 mg/kg, i.p,) and Lithium (25 and 50mg/kg i.p,)
treatment was given once a day, 1 hour before 3-NP treatment for 14 days. Tin (IV) protoporphyrin
(SnPP), HO-1 inhibitor was also administered with both hemin and lithium chloride. Behavioral
parameters were assessed on 1
st
, 5
th
, 10
th
and 15
th
day post-3-NP administration. Oxidative stress and
endogenous antioxidant enzymes, proinflammatory [TNF-, IL-1], HO-1 and GSK-3 activity
were measured in the striatum. 3-NP treatment produced significant behavioral abnormalities and
increase in oxidative stress in striatal brain. Administration of hemin (10 and 30 mg/kg, i.p), Lithium
(50mg/kg i.p,) prevent the alteration in body weight, motor impairments, oxido-nitrsoative stress,
and cellular markers. Further, combination of hemin (10mg/kg) and lithium chloride (25mg/kg) were
showed synergistic effect as compared to their effect alone on 3-NP treated rats. Pretreatment of Tin
(IV) protoporphyrin(40 M/kg) reversed the beneficial effect of lithium chloride and hemin. The
outcomes of the present study suggest that HO-1 and GSK-3 enzyme are involved in the
pathophysiology of HD. The various modulators of both the enzymes like hemin and LiCl might be
adjuvants or addition or prophylactic therapy for HD patients.

PCL-15

FRACTIONS OF BUTEA MONOSPERMA LEAF ATTENUATE MPTP-INDUCED
BEHAVIORAL MOTOR DEFICIT AND OXIDATIVE STRESS IN RATS

PriyaJaswal*, PriyankaChhabra, Rahul Deshmukh
Department of Pharmacology, I.S.F College of Pharmacy, Ferozepur Road, GhalKalan, Moga
142001, Punjab, India
Email : priyajaswal138@gmail.com

Abstract:
Extracts of Butea monosperma has been reported to possess neuroprotective as well as
phosphodiesterase (PDE) inhibitory activity. In the present study, we have investigated PDE
inhibitory activity of petroleum ether and ethyl acetate fraction of leaves of Butea monosperma
August 22, 2014


and their neuroprotective potential against MPTP induced experimental Parkinsons disease in
rats. The MPTP was infused bilaterally (100mg/l) into substantia nigra pars compacta and the
test compound PEBM/EABMor L-DOPA was administered from1
st
day following MPTP
infusion up to 15
th
dayand the same treatment was done in standard treatment group of L-DOPA
(10mg/kg i.p). Behavior motor deficits in rats were evaluated by open field test, rota rod, grip
strength and narrow beam walk tests. Biochemically, markers of oxidative-nitrosative stress were
evaluated in striatal brain homogenate. Both fractions (PEBM/EABM) produced significant
cAMP/cGMP specific PDE inhibitory activity in in-vitro assay. MPTP infusioned rats showed
significant deterioration in behavior motor functions and increase in striatal oxidative stress in
rats. However, the fractions (PEBM/EABM) of Butea monosperma significantly and dose
dependently attenuated MPTP- induced behavior motor deficits and oxidative stress and results
were comparable to that of L-DOPA. The observed beneficial effects of PEBM/EABM may be
due to their antioxidant potential and may be due to their ability to inhibit cAMP/cGMP specific
PD.

PCL-16

POSSIBLE BENEFICIAL EFFECT OF PEROXISOME PROLIFERATOR-ACTIVATED
RECEPTOR (PPAR) - AND AGONIST AGAINST A RAT MODEL OF ORAL
DYSKINESIA

Swati Datta* , Sania Grover, Puneet Kumar, Vir Vikram, R.D. Budhiraja
Department of Pharmacology, I.S.F. College of Pharmacy, Moga-142001, Punjab, India
Email- dutta.swati90@gmail.com

Abstract:
Tardive dyskinesia is a type of hyperkinetic movement disorder which consists of abnormal
involuntary movements, characterized by orofacial movements. Previous studies suggest that
oxidative stress and neuro-inflammation play important role in the pathogenesis of TD. Recently,
PPAR- and PPAR- have been reported as neuroprotective agent in various animal models.
The present study investigated the neuroprotective effect of PPAR- agonist, pioglitazone (20
and 40 mg/kg, p.o.) and PPAR- agonist, fenofibrate (100 and 200 mg/kg, p.o.) in an animal
model of oral dyskinesia. Oral dyskinesia was induced by chronic administration of haloperidol
(1 mg/kg i.p.) for 21 days. Chronic administration of haloperidol significantly increased vacuous
chewing movements, tongue protrusions, facial jerking, sniffing and grooming in rats which was
dose-dependently inhibited by pioglitazone and fenofibrate. Further, it also decreased %
retention of memory in elevated plus maze test on day 22. Chronic administration of haloperidol
induced oxidative damage and neuroinflammation (TNF- and IL-1) in brain regions. The
fenofibrate and pioglitazone were able to reverse the behavioral and biochemical changes
induced by haloperidol. Further the study proposed the antioxidant and antiinflammatory effects
of both PPAR agonists in this model. We concluded that administration of pioglitazone and
fenofibrate individually or in combination along with antipsychotic in the treatment of
schizophrenia, prevent or delay the symptoms of oral dyskinesia.

August 22, 2014


PCL-17

ROLE OF FXR/H2S PATHWAY IN DINITRO BENZENE SULFONIC ACID (DNBS) -
INDUCED ULCERATIVE COLITIS IN RATS

TavleenKaur*, NidhiGoyal, Krishna Reddy V. Bijjem, Puneet Kumar,
Department of Pharmacology, I.S.F College of Pharmacy, Moga-142001, Punjab,India,
Email:prtavleen@gmail.com

Abstract:
UC is a chronic inflammatory condition in which the inflammatory response and morphologic
changes remain confined to the colon. There is a need to explore the new targets for UC such as
Farnesoid X receptor and hydrogen sulfide pathway. Wistar rats of either sex (200-250 gm) were
used. DNBS (25 mg/rat) was dissolved in 50% ethanol (total volume 0.8 ml) and given by rectal
route into the colon to induced symptoms of UC. CDCA (10 and 20 mg/kg) and NaHS (10 and
30 mol/kg) and a inhibitor of CSE enzyme i.e PAG (10mg/kg) treatment given along with
DNBS. The disease activity index was assessed by daily change in body weight and rectal bleed
score and change in length of colon. Oxidative stress markers (reduced glutathione, MDA,
nitrite, and catalase and myeloperoxidase enzyme activity), SGOT and SGPT levels in blood
serum, and cardiac haemodynamic were performed on last day. The administration of DNBS
intra-rectally in rats produced significant loss of body weight and bloody diarrhoea with
significant increase in oxidative stress markers in the colon. CDCA (10 and 20 mg/kg) and NaHS
(10 and 30 mol/kg) significantly attenuated DNBS-induced UC in rats. The combination of
CDCA (10 mg/kg) and NaHS (10 mol/kg) showed synergystic effect whereas; DL-propargyl
glycine reversed the protective effect of CDCA. CDCA and NaHS showed beneficial effects
dose-dependently against DNBS-induced UC in rats. The observed beneficial effects following
CDCA may be due its action through activation of CSE enzyme which leads H
2
S generation.

PCL-18

BENEFICIAL EFFECT OF SPHINGOSINE-1-PHOSPHATE RECEPTOR ANALOG AS
ANTI-ARTHRITIC AND ANTI-OSTEOPOROTIC IN CFA INDUCED
OVARIECTOMIZED RATS

Vandana*,Shilpi Sachdeva, Krishna Reddy V. Bijjem, Puneet Kumar
Department of Pharmacology, ISF College of Pharmacy, Moga-142001, Punjab,India,
Email: vandana2556459@gmail.com

Abstract:
Rheumatoid arthritis (RA) and osteoporosisare chronic diseases characterized by fibroblastic
proliferation, infiltration of the synovial lining by inflammatory cells and increase bone
resorption respectively.Sphingosine -1-phosphate (S1P) receptor analoghas unique pathogenic
mechanistic role which can be beneficial to reduce the side effects and improve the efficiency for
the treatment of rheumatoid arthritis and osteoporosis. Osteoporosis and rheumatoid arthritis are
August 22, 2014


induced by ovariectomy and single injection of CFA in rats. On 16
th
, 23
rd
, 30
th
, 37
th
day after
ovariectomymechanical allodynia, thermal hyperalgesia, paw volume, joint stiffness and
mobility were observed. On 37
th
day animal sacrificed for rheumatoid factor, calcium and
phosphate and haemoglobin count in serum were determined. Ovariectomy and CFA
significantly induced symptoms of osteoporosis and RA in rats like joint stiffness, decrease in
mobility, paw oedema, mechanical allodynia and thermal hyperalgesia. Increase in rheumatoid
factor and decline of hemoglobin, calcium and phosphate count was also observed after
sacrificing the animals. FTY720 (0.1 and 0.3 mg/kg), methotrexate (0.3 mg/kg) and diclofenac(5
mg/kg) attenuated all the behavioural and haematological parameters alone and/or combination
as well as alone. In the present set of experiments FTY720 has shown significant anti-arthritic
and anti-osteoporotic effect in rats. Further, when FTY720 low dose was combined with standard
drugs like methotrexate and diclofenac showed the synergistic effect.

PCL-19

EVALUATION OF THE ANTI-INFLAMMATORY PROPERTY OF COMBINED
THERAPY OF CHOLINERGIC ANTAGONIST SCOPOLAMINE AND VENLAFAXINE
IN RATS.

Paramdeep Singh*, Raghav Gupta
Chitkara College of Pharmacy, Chitkara University, Chandigarh-Patiala National Highway,
Rajpura 140401, Patiala, Punjab, India.
E. Mail: paramdeep.singh@chitkara.edu.in

Abstract:
The present study has been designed to investigate the effect of combined therapy of cholinergic
muscarinic receptor antagonist scopolamine and venlafaxine using carrageenan induced
inflammation model in rats. Our experimental protocol consisted of administration of 0.1 mL of
2% w/v carrageenan in normal saline for inducing inflammation in rat paw. Ibuprofen (30
mg/kg) used as standard was given orally 30 minutes prior injecting carrageenan. Treatment
consisted of venlafaxine per se (20 mg/kg, p.o.) and scopolamine (0.2 mg/kg, i.p.) in
combination with venlafaxine. Inflammation of the paw was measured using plethysmometer
after 2 and 24 hrs of injecting carrageenan into the paw. Combined treatment was found to
decrease the paw volume compared to ibuprofen and venlafaxine per se. This decrease in
inflammation was significant (p<0.05) compared to vehicle control in rats. The present study
suggested that combined therapy of scopolamine and venlafaxine, may serve as a viable future
pharmacological target to tackle the problem of inflammation. More research on this topic is
needed to pinpoint the type of receptors involved in showing ameliorative action on
inflammation.

August 22, 2014


PCL-20

REVIVAL OF THALIDOMIDE TRANSFORMS IT INTO A VERSATILE NOVEL
THERAPEUTIC AGENT

Ankur Gera
1*
,Chander Mohan
2
and Sandeep Arora
1
1
Chitkara University , Chitkara College of Pharmacy, Rajpura, Patiala-140401, Punjab, India.
2
Rayat-Bahra Institute of Pharmacy, Hoshairpur, Punjab, India.
E-mail: ankur112@gmail.com

Abstract
Thalidomide was withdrawn from the market in 1961, because of its teratogenic effects. Insights
gained from the development of thalidomide has helped to pave the way for the development of
other novel therapeutic agents. Structural and functional modifications led to promising new
analogues that modulate the immune system in various ways, so the immunomodulatory effects
can be used for the treatment of various inflammatory, autoimmune, and neoplastic diseases. In
clinical studies, the IMiDs appear to have reduced sedative and neurotoxicity effects, which are
often associated with long-term thalidomide dosing. The future of this class of compounds is in
the more-potent IMIDs, particularly lenalidomide, where the significantly increased
immunomodulatory and anti-angiogenic potency and apparent lack of some, or a decreased
amount of, thalidomides dose-limiting side effects have made them potentially important
therapeutics in cancer and inflammatory diseases. This arena can be explored to get safer and
selective drugs.

PCL-21

SLEEP APNOEA: LIFE THREATENING BUT LIMITED AWARENESS

Kapil S
*
, Singhal R, Arora S
Chitkara College of Pharmacy, Chitkara University

Abstract:
Sleep Apnoea (Obstructive Sleep Apnoea and Central Sleep Apnoea) is sleep disordered
breathing caused due to obstruction in airway at night. This leads to serious drop in blood
oxygen level thus causing cardiovascular disorders and nocturnal death. The objective of this
work is to spread awareness about this disease as many people fail to recognize the symptoms
and are unaware of the fact that they are suffering from this disorder. If one encounters loud
snoring followed by gasping, consult a sleep specialist immediately. OSA suffering person will
also suffer with sleepiness during day, lack of concentration, mood swings, depression, less
learning ability, weak memory. Motor sleepiness might also occur! Diagnosis includes physical
examination by checking presence of extra tissue in throat. PSG (Polysomnography) records
blood oxygen level, brain activity, heart activity like BP, heart rate, etc during sleep at night for
each second. Treatment includes CPAP (Continous Positive Airway Pressure), VPAP (Variable
Positive Airway Pressure), APAP(Auto Adjusting Airway Pressure) machinesand surgery in
August 22, 2014


some cases. There is no medicinal therapy for OSA. Since OSAis life threatening disorder,
require very expensive therapy, and have limited awareness among people as well as physicians.
Thus, research in this field should be encouraged so that people can take precautions in their
daily life and mortality rate will also be reduced.

PCL-22

DIABETIC WOUND CARE AND MANAGEMENT

Vipul gogar, Minali Mishra, Piyush Khandelwal, Deepika Deopa
*
Suresh Gyan Vihar School of Pharmacy Jaipur Rajasthan

Abstract:
Diabetes is an increasingly common problem and often results in severe morbidity and economic
burden. Foot infection is a common problem in diabetic patients. Hyperglycemic condition
impairs wound healing in diabetic foot ulcers which is the leading cause of wound amputation.
The abnormality in local blood flow, apoptosis, regeneration and defense mechanism contribute
to lack of healing. A series of mechanism are involved in wound healing. The pathophysiological
changes were studied in case of normal and diabetic wounds. Herein we delineate several
approaches that are under standard care practices and technological breakthroughs in the field of
diabetic wound care.

PCL-23

AUTOIMMUNE DISORDERS AND ALTERNATE THERAPIES

Rakesh K Sindhu, Sandeep Arora and Jasmina Kapur*

1
Chitkara College of Pharmacy, Chitkara University, Rajpura, Pataiala, Punjab - 140401

Abstract:
Autoimmune disorders occur when the body's immune system turns against the body itself,
attacking as if it were a foreign pathogen. They comprise more than 50 distinct diseases and
syndromes, and affect about 5% of the population in Europe and North America, with two thirds
of the patients being female
.
Autoimmune disorders include rheumatoid arthritis, multiple
sclerosis, juvenile diabetes, cardiomyopathy, antiphospholipid syndrome, Guillain-Barr
syndrome, Crohn's disease, Graves' disease, Sjogren's syndrome, alopecia, myasthenia gravis,
lupus erythematosus, and psoriasis. Arthritis alone is estimated to cause a $65 billion disease
burden , so autoimmune disorders as a group are among the most expensive diseases faced by
society today. As a result, they are the subject of significant research in both academic and
industrial laboratories. The treatment of autoimmune diseases is the prescription of the western
medicines which are strongest medication available that kills the immune system without
investigating the causes of its malfunction. Not surprisingly, the effectiveness of such a primitive
approach is usually minimal and maximum side effects. So, alternate therapies play important
August 22, 2014


role in the successful treatment of autoimmune disorders. This includes, the fight against stress,
toxicosis and microbes, vitamins, minerals, hormones, individualized diet and enzymes etc.

PCL-24

SIGMA RECEPTORS- A MILESTONE IN THE TREATMENT OF NEUROPSYCHIATRIC
DISORDERS

Siddharth Sharma*and Ritchu Babbar

Chitkara College of Pharmacy, Chitkara University, Rajpura (PB.)
Email: - simpho.sharma@gmail.com

Abstract
Sigma receptors, initially proposed to be of subtype of opioid receptors are now confirmed to be non-
opioid receptors that bind diverse classes of psychotropic drugs. Sigma receptors have been classified into
two subtypes namely sigma 1 and sigma 2. Ligands, which bind to sigma receptors, exert their action
through activation of multiple neurotransmitter system. The sigma 1 receptor, one of two sigma receptor
subtypes is a chaperone protein at the endoplasmic reticulum that modulates calcium signaling through
the IP3 receptor.Sigma 1 receptor is predominantly expressed in deeper lamina of the cortex,
hypothalamus, olfactory bulbs and purkinje cells of the brain. Sigma receptors play an important role in
various neuropsychiatric illness like- depression, schizophrenia, anxiety disorders, substance use
disorders, somatic pain, and dementia..A variety of specific physiological functions have been attributed
to the sigma 1 receptor. Chief among these are modulation of Ca
2+
release and inhibition of voltage gated
K
+
channels. Activation of sigma 2 receptors has also been hypothesized to show anti cancer activity
though its apoptotic activity. Activation of sigma 1 and sigma 2 receptors can cause strong antidepressant
and anxiolytic actions. Many psychoactive drugs have shown affinity towards sigma receptors including
various anti depressant and anxiolytic drugs
(fluvoxamine,opipramol,sertraline,citalopram),antipsychotics(haloperidol),anticonvulsants(lamotrigine,ph
enytoin) and psychostimulants (amphetamine, methylphenidate).

PCL-25

ETHNO-PHARMACOLOGICALLY USED PLANTS FOR MALARIA TREATMENT IN
NORTHERN INDIA

Sumit Sachdeva*, Shiv Kant Sharma, Dhirender Kaushik
Email: sachdeva.sumit33@gmail.com

Abstract
Malaria, the killer disease, caused by single-celled protozoan parasites called Plasmodium and
transmitted to man through the Anopheles mosquito. Malaria continues to cause morbidity and
mortality on a large scale especially in the tropics, and is endemic in some 102 countries, with
more than half of the world population at risk with fatality rates being extremely high among
young children below 5 years of age. The World Health Organization estimates that there are
August 22, 2014


between 300 and 500 million new cases of malaria worldwide, every year, mostly in Africa,
Asia, South Pacific Islands and South America, which causes at least 1 million deaths annually.
Spread of multidrug-resistant strains of Plasmodium and the adverse side effects of the existing
anti-malarial drugs have necessitated the search for novel, well-tolerated and more efficient anti-
malarial drugs that kill either the vector or the parasite. Since plants are considered valuable
resources for obtaining the lead for the development of new anti-malarials. As a matter of fact,
quinine and artemisinin, the two most effective anti-malarials against drug-resistant P.
falciparum, were originally derived from the plants. 80% of people in developing countries rely
on traditional medicines. In India alone, about 2,500 species of plants belonging to 1,000 genera
presenting 250 families are used in traditional medicine. Present study explores the major plants
used ethno-pharmacologically for the treatment of malaria.

PCL-26

NUTRACEUTICALS ANTIOXIDANTS EFFECTS ON PESTICIDE TOXICITY
STUDIES

Dhirender Kaushik, Rahul Gupta*, Ajay Aggarwal, A.C Rana,

Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra 136119, Haryana.

Email- pr.rahulgupta146@gmail.com

Abstract
Pesticides are chemical substances that are used to kill, repel, or control the growth of natural
organisms. Pesticides occupy a unique position among the many hazardous chemicals that men
and animals encounter daily. In the present study Malathion is one of the most widely used
organophosphate pesticide for agriculture and community health programs for controlling pest
was taken to study its toxic effects on different organs of male wistar rats. In this study, we
investigated to evaluate different parameter such as physical parameter, weight parameter, blood
parameter, sperm count method and In-vitro method and histopathology of different organs. Two
different fruits were taken for the evaluating the antioxidant studies such as Punica granatum
and Citrus limonjuice. Thirty six rats were used in this study and classifiedinto 6 groups(6
animals per group). One dose was selected for the study, 400 mg/kg (1/3.8
th
of LD
50
). Standard
dose of 200 mg/kg of Ellagic acid and 100 mg/kg Vitamin C was taken to study the ameliorative
effect of Punica garantum andCitrus limonjuice on Malathion generated toxicity in different
organs, blood and sperm. Some of variations observed in different organs concluded that
malathion significantly affected the organs like liver, kidney, brain, testis and antioxidants
ameliorated the toxicity produced by the pesticide significantly.

August 22, 2014


PCL-27

DIETING IN OBESITY: IS IT REALLY EFFECTIVE?

Ankur Garg*, Dhirender Kaushik, Manjusha Chaudhary, A. C. Rana
E- mail: ankurgarg4306@gmail.com

Abstract
Obesity is a medical condition in which excess body fat has accumulated to the extent that it may
have a negative effect on health, leading to reduced life expectancy. People are considered obese
when their body mass index (BMI) exceeds 30 kg/ m
2
. Obesity increase the likelihood of various
diseases, particularly heart diseases, type 2 diabetes, obstructive sleep apnea, certain cancer and
osteoarthritis. Dieting and exercising are the main treatments for obesity. However, anti-obesity
drugs may be taken to reduce appetite or decrease fat absorption. But the most popular means of
all used widely is dieting. The FAD diets and very low calorie are most popular among social
media nowadays. But research shows that dieting when entertained irrationally generates various
negative physical and psychological behavioral patterns like binge eating, preoccupations with
food and hunger, depressed mood, irritability, decrease social ability and apathy. Moreover,
various pathological consequences are hair loss, fatigue, constipation, nausea, diarrhea and
increased risk of gallstones.

PCL-28

CHEMOTHERAPY AND IMMUNOTHERAPY FOR TUBERCULOSIS

Amandeep Thakur*, Priya Sharma
LR Institute of pharmacy, Solan
Email:- thakur.amandeep95@gmail.com

Abstract
Tuberculosis (TB) is a mycobacterium infection which occurs due to Mycobacterium
tuberculosis. It is the major infectious diseases with a mortality rate of nearly two million mostly
in developing countries every year. This increases occurrence of resistance of Mycobacterium
tuberculosis strains to the most effective antibiotics which serves as a major factor contributing
to the current TB epidemic. This situation has lead to a rise in the need of the development of
chemotherapy and immunotherapy. Chemotherapy is unique two phase therapy which consist of
first line and second line drugs. The drugs like Isoniazid, Ethumbutol etc. (First line ) and
ofloxacin, kanaycin etc.(Second line) are commonly used. These drugs used more effective and
easily available. The first line drugs are more effective than the second line drugs. Combination
drug therapy is desirable as a single drug is ineffective in the cases of MDR-TB. The commonly
known treatment of TB is DOTS. The immunotherapy show good result in MDR-TB. The use of
immunotherapy with Interlukin-2, Interferon and Interlukin-7 as an adjacent to drug treatment
may improve success rate of MDR- TB shortens the treatment time for drug sensitive TB and
August 22, 2014


improves the immunity by enhancing Mycobacterium tuberculosis elimination to prevent the
recurrence of disease.

PCL-29

PARKINSONISM- A NEURODEGENERATIVE DISORDER

Priya Jaswal*, Mrs. Anita Rani Shiksharthi
LR College of Pharmacy, Solan.
Email: priya21jaswal@gmailcom

Abstract
Parkinsonism is an umbrella term that describe many conditions which share some of the
symptoms of Parkinsons.So the main leading symptoms of parkinsonism are : Tremors,
Bradykinesia, Rigidity,Postural instability and also including paresthesia and Orthostatic
hypotension etc. The basic cause behind the parkinsonism is brain cell death which occurs due to
the deficiency of dopamine in striatum which control muscle tone and co-ordinate body
movements. Parkinsonism is the second most common neurodegenrative disorder after
Alzheimers disease. The prevalence of parkinsonism is about 0.3% of the whole population in
industrialized countries.Caffeine consumption appear to be protective against parkinson disease
with a great decrease in this occuring with a large intake of caffienated bevarages such as coffee.
Belladonna alkaloids had been emperically used in parkinsonism. Mainly the drugs used in
parkinsonism are classified into two systems and the systems are: Dopaminergic system and
cholinerguc system. The dopaminergic system includes levodopa, bromocriptine, tolcapone and
amantidine etc and the cholinergic system includes procyclidine, promethazine etc. There are
some advanced future plans for treating and defeating parkinsons disease and they are: gene
therapy, neural transplantation, advance in deep brain stimulation ,medicine that provide
sustained effect throughout the day. According to WHO classification of drugs, antiparkinsonism
drugs have been assigned ATC code No.4.

PCL-30

GOUT: A COMMON FORM OF ARTHRITIS

Riya Thakur*, Mrs. Anita Rani Shiksharthi
LR College of Pharmacy, Solan
Riyathakur876@gmail.com

Abstract
Gout is a form of acute arthritis that causes severe pain and swelling in the joints. It most
commonly affects the big toe, but it may also affect the heel, ankle, hand, wrist and elbow. Gout
is different from other forms of arthritis because it occurs when there are high levels of uric acid
circulating in the blood, which can cause urate crystals to settle in the tissues of the joints. Gout
is a metabolic disorder characterized by hyperuricaemia. Gout patients are advised to restrict
August 22, 2014


proteinaceous diet as it leads to increased uric acid level in the body and they are advised to take
low fat dairy products, high vitamin C food and foods containing essential fatty acids etc. An
excess of uric acid in the blood brings causes gout. Most common factor that increases the
chances of developing gout is excess consumption of alcohol, particularly beer because it is high
in purines. A gout attack may lasts several days but usually goes completely within 7 to 10 days.
It is more common in men than in women. Gout has been classified into two main types: Acute
gout and Chronic Gout. Further to be discussed in the poster is the medication for gout and the
lifestyle changes that a gout patient should make to lead a healthy life.

PCL-31

HISTOLOGICAL STUDY OF MALATHION TREATED INTESTINE IN WISTAR
RATS

Shiv Kant Sharma
1
*, Rajnesh Kumar Sharma
2
, Dhirender Kaushik
1

1
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119, India
2
Department of Zoology, Kurukshetra University, Kurukshetra-136119, India
Email: shivkantvats@gmail.com

Abstract:
Malathion [O,O-dimethyl-S-(1,2-dicarcethoxyethyl) phosphorodithioate] is an organophosphate
pesticide which is extensively used worldwide, especially in developing countries, in agriculture
and household products for pest control. Malathion is known to inhibit acetylcholinesterase
activity in the target tissues and has been associated with the dysfunction of several organ systems.
The objective of the study was to determine the effect of Malathion on histology of intestinal
system of Wistar rat. Sexually mature Wistar rats were exposed to two doses (100 mg/kg) and (500
mg/kg) dose of Malathion orally for 5 days. Intestine was removed and fixed in aqueous Bouins
fixative for 48 hours and washed for 1-2 hours in running tap water. After dehydration through
series of alcoholic grades, the tissues were embedded in paraffin wax (melting point 60-62
0
C).
The sections were cut at 5 m thickness. Dewaxing was carried by xylene for 15-20 min. After
removal of wax, the slides were transferred to series of alcoholic grades and then stained with
haemotoxylene and eosin. Finally sections were mounted with DPX studied for histopathological
changes. Light microscopy study revealed Malathion produces severe histological changes in
intestinal tissue in a dose dependent manner.

August 22, 2014


PCL-32

ELEVATED TUNNEL MAZE: INDIAS OWN MAZE FOR MEASUREMENT OF
ANXIETY

Neeraj Gilhotra
1
* and Ritu Gilhotra
2
1
Pharmacology Laboratory, Department of Pharmaceutical Sciences,
Maharshi Dayanand University, Rohtak 124 001, Haryana, India
2
School of Pharmacy, Gyan Vihar University, Jaipur - 302 025, Rajasthan, India
Email: neerajmdu@rediffmail.com

Abstract
Elevated tunnel maze is a novel maze that may be employed to measure anxiety- like behavior in
mice. The design of tunnel maze offers a defined aversion for mice to exhibit differentiated
anxiety- like behaviors; (a) motor transition to different arm(s) (open or covered) of maze, (b)
unprotected head dip(s) from open arm of maze (uHDIPS), (c) protected head dip(s) from closed
arm(s) of maze (pHDIPS), and (d) stretched attend postures (SAPs) on the maze. Physically,
tunnel maze comprises a white straight platform containing a middle arm (16 5 16 cm)
covered with a roof (covered arm), and two terminal open arms (16 5 cm) on both sides of
covered arm. A 6 mm high lip is positioned on the boundaries of open arms that act as a tactile
clue for mice. A significant anxiety- like behavior was exhibited by mice during its stay on
elevated tunnel maze. A significant potentiation of anxiety- like behavior was observed in mouse
on tunnel maze after forced immobilization. These observations indicate the possible usefulness
of elevated tunnel maze as a novel and potentially reliable animal maze for measurement of
anxiety- like as well as antianxiety- like behavior of mice.

PCL-33

DIMINISHED ANTI-ANXIETY EFFECT OF DIAZEPAM IN STRESSED MICE
UNDER INFLUENCE OF P38MAPKINASE

Vipin Sharma
1
, Sezal
*2
, Ritu Gilhotra
3
and Neeraj Gilhotra
4
1
2
Pharmacology Laboratory, Department of Pharmaceutical Sciences, Maharshi
Dayanand University, Rohtak 124 001, Haryana, India
3
4
Dayanand University, Rohtak 124 001, Haryana, India.

Abstract
The present study aimed to explore the possible nitriergic influence and role of p38MAPK (p38
mitogen activated protein kinase) in the diminished anti-anxiety effect of diazepam in stressed
mice, using the elevated plus maze and light/dark box. Immobilization stress for 6h enhanced an
anxiety- like behavior and increased plasma nitrite levels in mice. Diazepam (2 mg/kg, i.p.)
August 22, 2014


produced an anti-anxiety effect in unstressed mice, but could not produce any change in anxiety
levels of stressed mice. SB-203580 (2 mg/kg, i.p.), a specific inhibitor of p38MAPK, per se
produced a significant anti-anxiety like activity in stressed mice. Administration of a
combination of SB-203580 (2 mg/kg, i.p.) and diazepam (2 mg/kg) in stressed mice produced a
significantly higher anti-anxiety like activity than that produced by SB-203580 alone. Diazepam
could not produce any change in plasma nitrite levels in both unstressed and stressed mice. SB-
203580 (2 mg/kg, i.p.) significantly decreased plasma nitrite levels in stressed mice. The
observations indicate that the diminished anti-anxiety effect of diazepam in stressed mice may
involve strong nitriergic influence and may further be p38MAPK- dependent.

PCL-34

ALDOSE REDUCTASE INHIBITORS FOR MANAGEMENT OF DIABETIC
COMPLICATIONS

Ajmer Singh Grewal
Department of Pharmaceutical Chemistry, JCDM College of Pharmacy, Sirsa, Haryana
E-mail: ajmergrewal2007@gmail.com

Abstract
Diabetes is a metabolic disorder characterized by hyperglycemia, which has become global
health burden because of the complications associated with it. The polyol pathway is of prime
importance in the pathogenesis of diabetic complications. Aldose reductase, the rate-controlling
enzyme in the polyol pathway, is the potential target for the treatment of diabetic complications.
Therefore, inhibition of aldose reductase is an attractive approach in the management of diabetic
complications. The inhibitors of aldose reductase developed vary structurally, carboxylic acid
derivatives are the largest class. Examples include Alrestatin, Epalrestat, Tolrestat, Zopalrestat,
Zenarestat, Ponalrestat, and Lidorestat. The aldose reductase inhibitor, Epalrestat is marketed in
Japan, China and India. In addition, some other aldose reductase inhibitors had been advanced
into late stage of clinical trials. The present paper article will give brief overview of the role of
aldose reductase in the diabetic complications along with discussion on aldose reductase
inhibitors developed recently and their potential use in the treatment and management of the
major diabetic complications such as cataract, retinopathy, neuropathy and nephropathy.

PCL-35

DOTS THERAPY FOR TUBERCULOSIS

Bhawna Vaish*, Dhirender kaushik, A. C. Rana, Manjusha Chaudhary
Email bhawnavaish99@gmail.com

Abstract
August 22, 2014


Tuberculosis is an infectious disease caused by bacteria Mycobacterium tuberculosis. It is spread
through air by infected person. A single patient can infect 10 or more people in a year. M.
tuberculosis is responsible for over 98% of infections. T.B. is a serious public health problem in
India. 1/5
th
of T.B. patients across the globe are in India and estimated 4 lacks deaths occur from
t.b. every year in India. But these deaths can be prevented with proper care treatment. Poor
adherence to therapy is a major factor responsible for prolonged illness, relapse and emergence
of resistance. To overcome these drawbacks, DOTS( Directly Observed Treatment Short course)
program has been started in which drugs are administered under the direct supervision of a
member of health team. It ensures that the patient has received the right drug in the right dose, at
the right interval and for the right duration. WHO DOTS strategies employing standardized
treatment for 6 months produces highest cure rates for drug sensitive T.B. The DOTS strategy
along with other component of stop T.B. strategy, implemented under the Revised National T.B.
control program (RNTCP) in India is a comprehensive package for T.B. control. The RNTCP,
based on DOTS strategy, began as a pilot in 1993 and was launched as national program in 1997.

PCL-36

ADVANCED THERAPIES IN CANCER TREATMENT: AN OVERVIEW

Minakshi Gupta*
2
, Jyoti Dahiya
1
2
, Harish Dureja
2
1
2

Abstract
Cancer is one of the most frequent and distressing diseases. The mortality caused by cancer and
its prevalance have increased during the last 50 years. Cancer is still one of the most destructive
disease for human beings due to its complexity and progressive nature and the clinical
management of this deadly disease continues to be a challenge for the 21
st
century. Various types
of cancer are reported in literature such as Carcinoma, Sarcoma, Lymphoma, Leukaemia,
Blastoma and Germ cell tumour. There is continuous need for new and better cancer therapies.
This review aims to present the various types, stages of cancer and also focuses on various
therapies used for the treatment of cancer. A few years ago, surgery and radiotherapy were the
only effective way to fight tumour growth. Now various therapies for the treatment of cancer
have been developed including chemotherapy, radiation therapy, proton therapy, thermotherapy,
photodynamic therapy, laser therapy, sentinel lymph node biopsy, cryotherapy and
differentiation therapy. These therapies have dramatically changed the scenerio of cancer
treatment. Further research is also needed to discriminate the various genes and signaling
pathways in the process of the carcinogenesis from cancer stem cells for development of novel
therapies, with the ultimate goal of eliminating the residual disease and recurrence.

August 22, 2014


PCL-37

POSSIBLE UNDERLYING INFLUENCE OF NF-B IN THE DIMINISHED
ANTI-ANXIETY EFFECT OF DIAZEPAM IN STRESSED MICE

Vipin Sharma
1
, Seema Chokar
2
, Ritu Gilhotra
3
and Neeraj Gilhotra
4
*

1
2
3
*
4

Abstract
The present study was designed to explore the possible nitriergic influence and role of NF-B in
the diminished anti-anxiety effect of diazepam in stressed mice, using the elevated plus maze and
light/dark box to assess anxiety. Immobilization stress for 6 h enhanced an anxiety-like behavior
and increased plasma nitrite levels in mice. Diazepam (2 mg/kg, i.p.) produced an anti-anxiety
effect in unstressed mice, but could not produce any change in anxiety levels of stressed mice.
Pyrrolidine dithiocarba- mate (PDTC), an inhibitor of the activation of NF-B, per se produced a
significant anti-anxiety like activity in stressed mice. Combination of PDTC and diazepam also
served to produce a higher significant anti-anxiety like activity in stressed mice than that
produced by PDTC alone. Diazepam could not produce any change in plasma nitrite levels in
both unstressed and stressed mice. PDTC (100 mg/kg, i.p.) significantly decreased plasma nitrite
levels in stressed mice. The observations indicate that the diminished anti-anxiety effect of
diazepam in stressed mice may involve strong nitriergic influence and may further be NF-B
dependent

August 22, 2014


PCL-38

DIFFERENTIAL MODULATION BY NEUROCHEMICALS OF ANXIETY
DISORDERS

Sant Lal
1
, Ritu Gilhotra
2
and Neeraj Gilhotra
3

1
2
*
3
Email-neerajmdu@rediffmail.com

Abstract:
Condition of anxiety affects the occurrence of even normal things in life. Chemical modalities
development requires specific role of biochemical(s) that may modulate the brain processes
leading to change in behaviour. The objective of the presentation is to project biochemical
involvement of different neurochemicals in control of expression of symptoms of anxiety. In
addition to established role of GABA, agents with possible anxiolytic activity have also been
found to affect the serotonin and norepinephrine systems. This paper suggests the potential
usefulness of different neurochemicals, their mechanism of biochemical disturbance and current
potential therapeutic targets in anxiety disorders.

PCL-39

DRUG INDUCED HEPATOXICITY

Sandeep kaur*,Dhirender kaushik, Manjusha chaudhary
Institute of Pharmaceutical Sciences,Kurukshetra university,Kurukshetra
Email:- sandykaur30.sk@gmail.com

Abstract:
Liver is the principle organ for maintaining the bodys internal environment. There is currently
no way to reimburse for the absence of liver function. Its major influence is on the flow of
nutrients and controls the metabolism of carbohydrate, protein and fats. Drugs are an important
cause of liver injury. More than 900 drugs, toxins, and herbs have been reported to cause liver
injury. Approximately 75% of the idiosyncratic drug reactions result in liver transplantation or
death. Various types of drug induced liver diseases are acute-dose dependent liver damage, acute
fatty infiltration, cholestatic jaundice, liver granulomas, active chronic hepatitis, liver cirrhosis,
liver tumors etc. In INDIA, approximately 2000 cases of acute liver failure occur annually and
August 22, 2014


drugs account for over 50% of them (37% are due to acetaminophen, 13% are idiosyncratic
reactions due to other medications). Drugs account for 2- 5% of cases of patients hospitalized
with jaundice and approximately 10% of all cases of acute hepatitis. Chronic liver disease and
cirrhosis account for some 2% of mean in 17 countries with nearly 40,000 deaths per year.
Considering the importance of drug-induced hepatotoxicity as a major cause of liver damage,
this review throws light on various drugs which induce hepatotoxicity, with their mechanism of
liver damage and clinical scenario.

PCL-40

RHEUMATOID ARTHRITIS CAUSES AND TREATMENT: REVIEW
Ojashvi Sharma*, Manjusha Choudhary
E-mail: ojashvi.1@gmail.com

Abstract:
Rheumatoid arthritis is an autoimmune disorder, characterised by chronic and erosive destruction
and deformity of the peripheral joints. About 3 million people in US have RA and mostly in
womens. Etiology of RA is still unknown but its known to b caused by Environmental factors,
Viruses, bacteria deposition and genetic predisposition, targeting the potential infections
involving the activation of CD
4
and T-cells which releases cytokines (TNF ,IL-1) which damage
the synovium vesicles and causes bone fibrosis and then joint deformities. It concluded as RA is
not a discrete clinical entity with a single etiological resource. Treg cells and anti-CD20 antibody
(rituximab) normally suppress inflammation by inhibiting proliferation and cytokine
production.HSV-6 and mycoplasma speices are also involved with some genetic linkages.This
represent various starting points which guides for the future aspects to treat RA and provide
future consequences for research and development of new meaningful therapeutic intervention
for this burdensome condition and this will not be an easy task.

PCL-41

PATHOGENESIS OF PEPTIC ULCER: A REVIEW

Abhishek dabra*, Manjusha choudhary
Institute of pharmaceutical science, kurukshetra university, kurukshetra
E-mail:- abhishekdabra87@gmail.com

Abstract
A peptic ulcer is a sore on the lining of the stomach or duodenum. The two most common type of
peptic ulcer are called gastric ulcer and Duodenal ulcerpeptic ulcer are found due to an
imbalance between aggressive factor such as hydrochloric acid (HCl) , pepsin, refluxed bile,
August 22, 2014


leukotrienes, reactive oxygen species and defensive factors, which include the function of
mucous bicarbonate barrier, prostaglandins (PGs)mucosal blood flow cell renewal and migration,
nonenzymatic and enzymatic antioxidants and some growth factors. H. Pylori infection and the
use of NSAIDs are the predominant cause of peptic ulcer disease. A number of factors are also
implicated in the pathogenesis of gastric ulcer. Among which major factor involved are bacterial
infection (Helicobacter pylori), certain medication (NSAIDs), chemicals (HCl/ethanol), gastric
cancer and minor factor are stress, smoking, spicy food and nutritional deficiencies. The idea
behind treating ulcer is to lower the amount of acid that your stomach make to neutralize the acid
that is made and to protect the injured area so it can have time to heal. The main of this review
has to summarize the ulcerogenic mechanism of various mediators involved in peptic ulcer
disease. Peptic ulcer disease remains a frequent clinical problem in our environment
predominantly affecting all age of people. As the prevalence of peptic ulcer disease increases
with advancing age it is expected that this common disease will continue to have a significant
global impact on health care delivery, health economics and the quality of life of patients.

PCL-42

MEMORY ENHANCING POTENTIAL OF POLYHERBAL FORMULATION:
PLAUSIBLE ROLE OF OXIDATIVE BIOMARKERS

Priyanka Pahwa
1*
, Rajesh Kumar Goel
1
, Dhirender Kaushik
2
1
2
Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra

Abstract:
The Present study was designed to investigate the memory enhancing potential of Polyherbal
Formulation and the role of oxidative biomarkers. Different groups of animals were administered
with repeated doses of polyherbal formulation (100 and 500 mg/kg, p.o.), Piracetam (400 mg/kg,
p.o.), Diazepam (1 mg/kg, i.p.), Mentat (200 mg/kg, p.o.) and vehicle for 15 days. The elevated
plus maze and passive avoidance apparatus served as exteroceptive behavioral models while
diazepam induced amnesia served as interoceptive behavioral models. On the 16
th
day, the
animals were sacrificed and brain homogenates were used for the estimation of acetyl
cholinesterase, TBARS and glutathione. In addition, certain serum estimation and oxidative
biomarkers were estimated. Treatment with the polyherbal formulation significantly (p<0.05)
reduced the transfer latency and increased step down latency as compared to vehicle control, in a
dose dependent manner. Moreover, remarkable changes were observed in acetyl cholinesterase,
TBARS, glutathione and oxidative biomarkers. The observed memory enhancing potential of
Polyherbal Formulation makes it a promising candidate for cognitive impairment.

August 22, 2014


PCL-43

STUDIES TO EXPLORE A COMPREHENSIVE POTENTIAL OF AGMATINE FOR
THE TREATMENT EPILEPSY AND ASSOCIATED COMORBIDITIES
Neetu*, Dinesh Gawande, Rajesh Kumar Goel
Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Punjab,
India.

Abstract:
Chronic anticonvulsant effect of agmatine using chemoconvulsant model was carried out using
Swiss albino mice (22-28 g). For ameliorative effect of agmatine treatment, fully kindled animals
were divided in different groups and challenged with PTZ shot intermittently (on day 5, 10 and
15) during the treatment period to mimic the full blown of grand mall epilepsy, After the each
challenged the seizure severity score was measured as well as after 3 hr the behavioural
evaluations were carried out to asses depression, anxiety and memory deficit.The chronic
treatment with agmatine resulted in significant reduction in seizure severity score in PTZ kindled
animals as compared to PTZ control animals. PTZ kindling is significantly associated with
depression, memory deficit and anxiety. Sodium valproate treatment reduced the seizure severity
score but could not improve the depressive behaviour and memory deficit in mice. Treatment
with agmatine 5mg/kg, 10mg/kg and 20mg/kg significantly reduced seizure severity score along
with the improvement in depressive behaviour and memory deficit. However, treatment with the
lower dose (10 mg/kg) appears to be more effective. The ameliorative effect of agmatine on
seizure severity score and associated comorbidities may be due to its inhibitory effect on NMDA
receptor and The present study concludes that agmatine effective in the management of epilepsy
as well as associated psychiatric comorbidities.

PCL-44

COMPARATIVE ANALYSIS OF EPILEPTIC BEHAVIOURAL CO-MORBIDITIES IN
PENTYLENETETRAZOLE KINDLED AND KINDLING RESISTANT MICE

Navjot Kaur, Tanveer Singh, Rajesh Kumar Goel
Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Punjab,
India.

Abstract:
45 Swiss Albino mice were kindled using PTZ (35 mg/kg i.p.), treated for 42 days on alternative
days. After chronic kindling animals were divided into two groups i.e. kindled group and PTZ-
resistant group (comprises of animals exhibiting no seizure after kindling period) along with
naive group. After last PTZ injection animals were evaluated for seizure severity score,
depressive behavior, memory deficit and anxiety. Resistance was found in 20% of animals. In
PTZ-kindled animals, significantly worsen learning abilities and depression were found as
compared to naive and PTZ-resistant animals. Whereas in case of anxiety, both kindled and PTZ-
August 22, 2014


resistant mice were found to be anxious as compared to naive. PTZ-kindling is associated with
resistance and kindling-resistant animals not showing psychiatric co-morbid depression and
memory loss.
PCL-45

MULTIPLE EXPOSURE TO DICHLORVOS AND MONOCROTOPHOS ON THE
BREATHING PATTERN AND RESPIRATORY VARIABLES IN RATS

Vinesh Choudhary
L.B.S. College of Pharmacy, Jaipur
Abstract
In the present study toxicity arising due to multiple exposures to dichlorvos and was analyzed.
Toxicity was studied by examining the breathing pattern using a computer generated program
that measures changes in the various respiratory variables at a time. Rats weighing around 200
gm were divided in different groups each of which was exposed to dichlorvos and
monocrotophos at different LD50 doses individually and in combination. AChE activity in brain
and serum for different group animals was also determined. There was a dose dependent
decrease in normal breath, suggesting involvement of toxicity in terms of broncho-constriction,
bronchorrhea, increased bronchial secretions with both dichlorvos and monocrotophos. However
it was more marked with monocrotophos than dichlorvos indicating that monocrotophos is more
toxic. In the present study monocrotophos exerts all effects maximally and therefore maximum
suppression of normal respiration was observed. Though co-exposure of DDVP and MCP was
expected to have more effect on AChE inhibition and respiratory failure, but un- anonymously it
was found that co-exposure of two showed antagonistic effect.

PCL-46

BOTTLE GOURD IS A NAURAL GUARD

Satbir Kaur*
Pharmacology Division, Institute of Pharmaceutical Sciences, Kurukshetra University,
Kurukshetra

Abstract
Lagenaria siceraria (Cucurbitaceae), popularly known as bottle gourd, louki or ghiya, is a
climbing plant, which bears hard-shelled and bottle-shaped gourds as fruits. Being rich in
vitamins, iron and minerals, it is forms an excellent diet for people having digestive problems.
Since it contains low calories, bottle gourd is an awesome foodstuff for shedding extra calories.
The fruit possesses diuretic, emetic, and refrigerant properties. Extract of the seeds show
antibiotic activity. The juice is helpful in constipation, premature graying hair, urinary disorders
and insomnia. Lagenaria siceraria juice were screened for its anti-nociceptive property using
both chemical and thermal methods of nociception in mice. Lagenaria siceraria juice was
August 22, 2014


administered at various concentrations ranging from 4%-16% v/v orally to Swiss mice (30g),
once daily for 15 successive days. The analgesic activity was measured using Tail Flick Method,
Tail Immersion Method and Acetic acid induced Writhing Test. Lagenaria siceraria juice
inhibited the abdominal constrictions induced by acetic acid and also increased the pain
threshold of mice towards the thermal source in a dose dependent manner. The activity exhibited
by the Lagenaria siceraria juice was comparable to that of the standard drug diclofenac (100
mg/kg/p.o). From the results it was concluded that Lagenaria siceraria juice exhibited anti-
nociceptive activity by central and peripheral mechanism(s). These findings reveal the analgesic
potential of ghiya.

PCL-47

RNA INTERFERENCE AND PERSONALIZED CANCER THERAPY

Kuldeep Vyas*, Shubhangi Chauhan, Dinesh Kumawat
Email: manudeep25@gmail.com

Abstract:-
Cancer is a complex disease both intrinsically and in relation to its host environment. From a
molecular standpoint no two cancers are the same despite histolytic similarity. As evidenced by
the recent advances in molecular biology, treatment for advanced cancer is headed towards
specific targeting of vulnerable signaling nodes within the reconfigured pathways created by
"omic" rewiring. With advancements in proteo-genomics and the capacity of bioinformatics,
complex tumor biology can now be more effectively and rapidly analyzed to discover the
vulnerable high information transfer nodes within individual tumors. RNA interference (RNAi)
technology, with its capability to knock down the expression of targeted genes (the vulnerable
nodes), is moving into the clinic to target these nodes, which are integral to tumor maintenance,
with a low risk of side-effects and to block intrinsic immunosuppressors thereby priming the
tumor for immune attack. An RNAi based sequential approach, a so called "one-two punch," is
being advocated comprising tumor volume reduction (ideally to minimal residual disease status)
effected by integrated multi-target knockdown followed by immune activation. Examples and
recent developments are provided to illustrate this highly powerful approach heralding the future
of personalized cancer therapy.

August 22, 2014


PHARMACHEMISTRY
ABSTRACTS

August 22, 2014


PCHEM-1

SYNTHESIS AND BIOLOGICAL EVALUATION OF 3-(4-(2-(ARYL)-4-
OXOTHIAZOLIDIN-3-YL)PHENYL-2-PHENYLQUINAZOLIN-4(3H)-ONES.

Samridhi*, Sandeep Jain
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and
Technology, Hisar, Haryana

Abstract
A number of 3-(4-(2-(aryl)-4-oxothiazolidin-3-yl)phenyl-2-phenylquinazolin-4(3H)-one
derivatives were synthesized by using anthranilic acid as a starting material. The structure of
entitled compounds (QT1-QT10) have been confirmed on the basis of various spectroscopical
techniques (IR,
1
HNMR) and analytical methods (R
f
, Melting point) as well as, all the
synthesized compounds were subjected to in vitro anticancer and antimicrobial activities.
ForIn vitro anticancer studies, two cell lines (MCF-7, Hep-G2) were used for evaluating
cytotoxic potential of synthesized derivatives by MTT assay method. QT4showed significant
cytotoxic activity against Hep-G2 cell line with IC
50
value 1.79 while other compounds did not
exhibit anticancer profile.Compound QT6-QT8 showed moderate cytotoxic activity against
MCF-7 cell line with IC
50
value 8.57, 8.54, 8.78 while QT5 showed significant cytotoxic activity
with IC
50
1.94. For In vitroantimicrobial studies, all compounds QT1-QT10 were evaluated
against two bacterial strains (B. subtilis, E. coli) and two fungal strains (C. albicans, A. niger)by
tube dilution method. QT3 has broad spectrum of antimicrobial activity. QT2-QT10 showed
remarkable activity against gram positive bacterial strain
B. subtilis but QT1 did not show any significant activity against B. subtilis. QT1-QT10 showed
very good activity against gram negative bacterial strain E. coli. QT3, QT7, QT9 QT10 showed
good activity against both fungal strains (C. albicans, A. niger) while QT4, QT5, QT6 were
totally inactive.

PCHEM-4

DESIGN, SYNTHESIS AND EVALUATION OF CHALCONE-THIAZOLIDINONE
HYBRIDS FOR ANTICANCER ACTIVITY

RuchikaGoyal*, Sunil Kumar, Ashwani Kumar, Sandeep Jain
Drug Discovery and Research Laboratory,Department of Pharmaceutical Sciences, Guru
Jambheshwar University of Science and Technology, Hisar, India

Abstract
A series of 3-(4-cinnamoylphenyl)-2-phenylthiazolin-4-one (chalcone-thiazolidinone hybrids)
has been designed, docked, synthesized in good yield and evaluated for anticancer activity on the
three cell lines A-549, U-87 and COLO-205. The designed compounds were first of all docked
into various receptors: Topoisomerase II (PDB: 1ZXM), JNKs [PDB: 1UKI (JNK1), 1JNK
(JNK3)], ER (3ERT) and ER (1X78), BChE (1EHO), COX-1 (20YE), COX-2 (3LN1), P-Gp
August 22, 2014


(3G60) involved in anticancer activity using in-silico tools (iGEMDOCK, AutoDockVina and
AutoDock), and then synthesized by the process involving green chemistry. All the compounds
showed significant cytotoxic effect in micro-molar range on the three cell lines. Compounds CT8
and CT6 were found to be the most potent on A-549 and COLO-205 (IC
50
= 38.89 M and 52.23
M) respectively. Observed IC
50
values on COLO-205 were found to be in good agreement with
theoretical binding affinity on COX-1 enzyme.

PCHEM-5

CHROMONEDERIVATIVES : AS ANTIDEPRESSANT AGENTS

Sonia Kohli*,Sukhbir L Khokra,DhirenderKaushik
Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra-136119
E-mail:- soniakohli19@yahoo.com

Abstract
Continuing our efforts on synthesis of some new chromone derivatives of potential biological
interest, we became interested in the synthesis of some new chromone derivatives for their
antidepressant activity. Chromone is a derivative of benzopyran with a substituted keto group on
the pyran ring. It is an isomer of coumarin.A literature revealed that substituted chromone have
received much attention during recent years on account of their prominent potential as
cardiotonic, analgesic and anti-inflammatory effects, antimicrobial, antibacterial, herbicidal
activities. Depression is a common neurological condition affecting 0.5 to 1% of the population
worldwide. The present review enumerates the results of different studies on chromone with
antidepressant properties and describes potential role of chromone nucleus in the development of
antidepressant agents.

PCHEM-6

SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF NEW SUBSTITUTED 2-(4-(5-
ARYLISOXAZOL-3-YL)PHENOXY)ACETIC ACID DERIVATIVES

Navidha*, Sandeep Jain
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science & Technology,
Hisar-125001, Haryana, India

Abstract
A number of substituted 2-(4-(5-arylisoxazol-3-yl)phenoxy)acetic acid derivatives were
synthesized by three step reaction: (i) Synthesis of chalcone derivatives from p-
hydroxyactophenone and substituted benzaldehyde (ii) the condensation of the Chalcone with
hydroxylamine hydrochloride (iii) the reaction of 3-(4-hydroxyphenyl)-5-aryl-isoxazole with
chloroacetic acid and sodium hydroxide. All compounds were obtained in appreciable yield
August 22, 2014


which were characterized by determination of various physicochemical parameters, IR and
1
H
NMR analysis. All the synthesized compounds were evaluated for in vitroantimicrobial activity
against two Gram negative StrainsEscherichia coli and Psuedomonasaeruginosa and Gram
positive strainBacillus subtilisand fungal strainCandida albicans and Aspergillusniger.All
synthesized compounds have a significant antimicrobial activity against the tested
microorganisms. Among the synthesized compounds IA
6
(2-(4-(5-(4-nitrophenyl) isoxazol-3-
yl)phenoxy)acetic acid ) were found to be the most active antimicrobial compound.

PCHEM-7

SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 2-(CHLOROMETHYL)-4-
PHENYLQUINOLINE-3-CARBOHYDRAZIDEDERIVATIVES

ManishaBishnoi, Rakesh K. Marwaha, SaloniKakkar and BalasubramanianNarasimhan
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak-124001
E-mail: mani.bishnoi10@gmail.com, naru2000us@yahoo.com

Abstract
Microbial infections are increasing at an alarming rate. Clinicians have to become reliant on few
antimicrobial drugs available in the market but that is not sufficient as microbial species are
getting resistant very fastly. In order to meet these challenges there is need for the development
of novel antimicrobial drugs to which the microbes have never been presented before So we have
synthesized a series of 2-(chloromethyl)-4-phenylquinoline-3-carbohydrazidederivatives and
evaluated them for their antimicrobial activity by tube dilution method against Gram positive
bacteria (Staphylococcus aureus, Bacillus subtilis), Gram negative bacterium (Escherichia coli)
and against fungus (Candida albicans and Aspergillusniger). The synthesized compounds having
electron donating substituents showed appreciable antimicrobial potentials. Antimicrobial
activity results indicated that Compound 6 (MIC
sa
= 0.61 x 10
-2
M/ml) was found to be most
potent antibacterial agent against S. aureus. Compound 1 (MIC
bs
= 0.68 x 10
-2
M/ml) was
found to be active againstB. subtilis. Compound 9 (MIC
ca
= 0.68 x 10
-2
M/ml) displayed most
potent antifungal activity against C. albicansand may be taken as lead compounds for the
development of novel antimicrobial agents.

PCHEM-8

3D QSAR AND DOCKING STUDY OF 2-((PYRIDIN-3-YLOXY) METHYL)PIPERAZINES
AS 7 NICOTINIC ACETYLCHOLINE RECEPTOR MODULATORS FOR THE
TREATMENT OF INFLAMMATORY DISORDERS

DeepikaPurohit
a
,Sanjiv Kumar
a
, AjitKumar
b#
andBalasubramanianNarasimhan
a$
a
Facultyof Pharmaceutical Sciences, MaharshiDayanand University, Rohtak-124001
b
Centre For Bioinformatics Centre, MaharshiDayanand University, Rohtak-124001
August 22, 2014


E-mail:
#
akumar.cbt.mdu@gmail.com,
$
naru2000us@yahoo.com

Abstract
Comparative Molecular Field Analysis (CoMFA) of 27 analogues of
2-((Pyridin-3-yloxy) methyl) piperazine derivatives was carried out using software Tripos
SYBYL X. Optimal r
2
(0.854) and q
2
(0.541) values were obtained for 3D QSAR model. The
contour plots obtained from CoMFA analysis have shown 13.84% steric contribution and
66.14% electrostatic contribution towards anti-inflammatory activity. 7Nicotinic Acetylcholine
Receptor is the reported target protein for anti-inflammatory activity. The homology model of
the receptor protein was generated in SWISS MODELLER using auto template mode and was
analysed for the quality using Procheck, QMEAN Z-score, Anolea and GROMOS plots. The
QMEAN score for the model was observed to be -3.862. The generated model of 7 Nicotinic
Acetylcholine Receptor was used for docking study of all 27 piperazine analogues using Auto-
Dock 4.2.5.1. The dock score obtained from docking analysis was then correlated with
experimental pIC
50
values for in-silico validation of the developed CoMFA model and a good
correlation was obtained with correlation coefficient (r
2
) value of -0.7378. The negative r
2
value
signifies that more is the pIC
50
, lesser is the dock score and thus better is the binding of ligand
with the receptor. The present investigation suggests an optimal 3D-QSAR with CoMFA model
for further evaluating new chemical entities based on piperazine skeleton.

PCHEM-9

SYNTHESIS AND ANTIMICROBIAL SCREENING OF N-(2-(ARYL)-4-
OXOTHIAZOLIDIN-3-YL)-2-(NAPTHALEN-2-YLOXY)PROPANAMIDES

Mukilkumar*, Sandeep Jain

Abstract
A series of someN-(2-(aryl)-4-oxothiazolidin-3-yl)-2-(napthalen-2-yloxy)propanamides were
synthesized by using -naphthol and 2-chloropropionic acid as a starting material. Firstly, 2-
(napthalen-2-yloxy) propanoic acid was prepared from -naphthol and 2-chloropropionic acid
which is after esterification with ethanol reacted with hydrazine hydrate to give rise to
corresponding hydrazides. Hydrazides were converted to corresponding Schiff bases which on
reaction with thioglycholic acid in presence of anhydrous zinc chloride and DMF to yield the
title compounds. The structure of title compounds (NT01-NT10) have been confirmed on the
basis of various spectral techniques (IR,
1
HNMR) and physicochemical methods (R
f
, Melting
point). All the synthesized derivatives were evaluated in vitro against two bacterial strains (B.
subtilis, E. coli) and two fungal strains (C. albicans, A. niger) by tube dilution method. NT01,
NT02, NT04 and NT05 showed remarkable ctivity against both bacterial (B. subtilis, E. coli.)
and fungal strains (C. albicans, A. niger). NT03, NT07 showed good activity against A. niger
strain only.

August 22, 2014


PCHEM-10

SYNTHESIS AND EVALUATION OF SOME 5-(4-((2-SUBSTITUTED-4-
OXOQUINAZOLIN-3(4H)-YL)IMINO)METHYLBENZYLIDENE)-1,3-
THIAZOLIDINE-2,4-DIONE DERIVATIVES FOR POTENTIAL ANTIMICROBIAL
ACTIVITY

AnshuPrabha Singh*, Sandeep Jain
Department of Pharmaceutical Sciences, GJUS&T, Hisar

Abstract
A series of 5-(4-((2-substituted-4-oxoquinazolin-3(4H)-yl)imino)methylbenzylidene)-1,3-
thiazolidine-2,4-dione derivativeshave been synthesized by the reaction of anthranilic acid and
different acyl chlorides or benzoyl chlorides and screened for potential antimicrobial activity.
All the derivatives were obtained in good yield and the purity of the compounds was confirmed
by R
f
value and melting point. The structures of the synthesized derivatives were confirmed on
the basis of their spectral data such as IR and
1
HNMR.
The antimicrobial activity of the synthesized compounds was tested
in vitro using serial dilution method against gram negative (Escherichia coli) and gram positive
(Bacillus subtilis, Staphylococcus aureus) bacterial strains and fungal strains (Aspergillusniger,
Candida albicans). All the compounds have displayed moderate to good antimicrobial activity.
The results revealed that the derivatives QA-11, QA-12,
QA-14 and QA-18 have shown good activity against B.subtilisand S.aureusand the derivatives
QA-14 and QA-18 exhibited remarkable activity against E.coli.
The derivatives QA-11 and QA-14 have displayed good antifungal activity against
A. nigerandC.albicans.

PCHEM-11

SYNTHESIS AND ANTIMICROBIAL EVALUATION OF N-(2-(ARYL)-4-
OXOTHIAZOLIDIN-3-YL)-2-(2-(2-METHYL-5-NITRO-1H-IMIDAZOL-1-
YL)ETHOXY)ACETAMIDE

Abhishek*, Sandeep Jain

Abstract
A series of new N-(2-(aryl)-4-oxothiazolidin-3-yl)-2-(2-(2-methyl-5-nitro-1H-imidazol-1-
yl)ethoxy)acetamide derivatives were synthesized by using metronidazole as a starting material.
Firstly 5-(2-methyl-5-nitro-1H-imidazole-1-yl)-3-oxopentanoic acid was prepared from
metronidazole and chloroacetic acid which is after esterification with ethanol reacted with
hydrazine hydrate to give rise to corresponding hydrazides. Hydrazides were converted to
corresponding Schiff bases which on reaction with thioglycholic acid in presence of anhydrous
August 22, 2014


zinc chloride and dry DMF to yield the title compounds. The purity of compounds was
ascertained by their physicochemical data (R
f
, Melting point) and structure of synthesized
compounds (MT01-MT10) were confirmed on the basis of their spectral data (IR,
1
HNMR). All
the compounds were evaluated against both bacterial and fungal strains. MT02, MT05
compounds showed remarkable activity against both bacterial (Bacillus subtilis, Escherichia
coli) and fungal strains (Aspergillusniger, Candida albicans). MT01, MT04, MT07, MT10
showed good activity against all the microbial strains.

PCHEM-12

SYNTHESIS AND PHARMACOLOGICAL EVALAUTION OF BENZOXAZOLE-
COUMARIN DERIVATIVES AS SAFE ANTI-INLFAMMATORY AGENTS

SwarandeepKohli
*
, SonaliSandhu, YogitaBansal, Gulshan Bansal
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002,
Punjab, India

ABSTRACT
Taking into consideration the importance of naturally existing coumarins as strong anti-oxidants,
the present study is undertaken to couple this nucleus with benzoxazole to develop safe anti-
inflammatory agents. Benzoxazole is core nucleus present in anti-inflammatory drug,
Benoxaprofen, which was withdrawn from clinics because of phototoxicity. The two series (4a-
e) and (5a-e) were designed and synthesized to obtain varied substituted benzoxazolecoumarin
derivatives. The series was evaluated for anti-oxidant, anti-inflammatory, ulcerogenic potential
and oxidative stress induction. Results of anti-oxidant activity study revealed compound 5e to be
most potent in comparison to test compound and standard drug, BHT. Anti-inflammmatory
activity was evaluated through in-vitro HRBC model and in-vivo Paw-edema model which
revealed that compound 5c exhibits maximum activity with 92.5% prevention of lysis and 52.7%
inhibition of edema. Compound 5c also possess significant anti-oxidant activity. Various
biochemical estimations and evaluation of ulcerogenic potential indicated compound 5c to be
maximally safe on gastric mucosa as well as induced negligible oxidative stress. Thus compound
5c may be taken as lead benzoxazole derivative for the development of safe anti-inflammatory
agents as they may not cause phototoxicity. This property may be attributed to the radical
scavenging ability of coumarin nucleus.

August 22, 2014


PCHEM-13

HYDROGEN SULFIDE RELEASING HYBRIDS A NOVEL APPROACH

Babita Devi*, PreetiRawat, Bhartendu Sharma.
L R College of pharmacy Solan
Babitanalwa47@gmail.com

ABSTRACT
The concept of hybrids in medicinal chemistry is not new and has been applied in several
therapeutic areas. In recent years, physiological roles of hydrogen sulfide have been recognized,
and there is emerging evidence that this endogenous gaseous substance can modulate
physiological processes. Moreover, hydrogen sulfide donors can increase the resistance of the
gastric mucosa to injury and accelerate repair. They are also known to produce cardioprotective
action and plays an important role in brain functions, probably acting as a neuromodulator as
well as an intracellular messenger. Furthermore, hydrogen sulphide inhibit smooth muscle
proliferation and platelet aggregation. Hydrogen sulphide is emerging as an important
endogenous modulator,because it exihibit the beneficial effects of nitric oxide on the
cardiovascular system without producing toxic metabolites. So taking into consideration the vast
application of hydrogen sulphide in the field of medicine, Its hybrids have been prepared and are
found to exhibit better activity as compared to conventional drugs. Few examples are
cardiovascular, H
2
S donating aspirin ; urology, H
2
S donating sildenafil ; and neurodegenerative,
H
2
S donating latanoprost for glaucoma treatment and H
2
S donating levodopa for Parkinson
disease. The method consists of combining appropriate chemical moeities carrying the hydrogen
sulphide group with the native drugs in order to obtain the best pharmacodynamic and
pharmacokinetic profile. The connecting linkers may range from aliphatic chains to
heteroaromatic rings.

PCHEM-14

DESIGN, MOLECULAR DOCKING AND SYNTHESIS OF 2-(2-
HYDRAZINYL)THIAZOLE DERIVATIVES AS POTENTIAL ANTI-MALARIAL
AGENTS

Dhirender Kaushik
1
, DeepikaPaliwal
*1
, Shilpy Aggarwal
2

1
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, 136119, Haryana
2
R.K.S.D. (PG) College Kaithal, 136 027, Haryana
Email: deepika14paliwal@gmail.com

Abstract
Malaria is one of the eminent threats and challenges facing our mankind presently. Malaria
parasite having developed multiple drug resistance to clinically established drugs, there is a
compelling need to introduce new chemical entities that can overcome the resistance. In present
August 22, 2014


work, we have designed2-(2-hydrazinyl)thiazole derivatives as anti-malarial agents. Lipnskis
Rule of five parameter and toxicity parameters were predicted through online servers like
Molinspiration and Osiris property explorer. To understand the mechanism, the designed
compounds were first of all docked into various receptors involved in anti-malarial activity using
in-silico tools like Autodock 4.2. Docking parameters such as binding free energy values of the
designed analoques were compared with standard drugs and those compounds having high
affinity towards receptor were synthesized using Hantzschthiazole synthesis method. This
involves reaction betweenthiosemicarbazoneand , -dibromoacetophenone. The chemical
structures of all the synthesized compounds were confirmed by IR and
1
H NMR spectral data.
All synthesized compounds were screened for their anti-malarial activity using in-vitro method.

PCHEM-15

HANSCH ANALYSIS OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES
OF SUBSTITUTED 1-ALKYL/ARYL-3-ETHOXY CARBONYL-5-HYDROXY-2-
METHYL INDOLES

Pradeep Kumar
1
*, Balasubramanian Narasimhan
2
and Ruma Saharan
3

1
Doon Valley Institute of Pharmacy and Medicine, Karnal, Haryana 132001, India.

2
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak - 124 001, India.
3
Advanced Centre for Biotechnolgy, MaharshiDayanand University, Rohtak 124 001, India.
Email: pradeepyadav27@gmail.com

Abstract
The anti-inflammatory and analgesic activities of substituted 1-alkyl/aryl-3-ethoxy carbonyl-5-
hydroxy-2-methyl indoles were correlated with their physicochemical parameters using Hansch
analysis. The multiple linear regression (MLR) model developed for anti-inflammatory activity
indicated the importance of lipophilic parameter, log of octanol/water partition co-efficient (log
P) and electronic parameter, energy of lowest unoccupied molecular orbital (LUMO) in
describing the anti-inflammatory activity. The QSAR model developed for the analgesic activity
demonstrated the importance of topological parameter, Kiers alpha shape index of first order
(
1
) in describing the analgesic activity. The developed QSAR models were cross-validated by
leave-one-out technique.

August 22, 2014


PCHEM-16

DESIGN AND SYNTHESIS OF QUINOLINYL PYRAZOLES AS DUAL INHIBITOR OF
FALCIPAIN-2 & DIHYDROFOLATE REDUCTASE FOR ANTIMALARIAL
POTENTIAL

Sandeep Jain
1
, Deepika Saini
1
*, Dhirender Kaushik
2
, Ajay Kumar
2
1
Department of Pharmaceutical Sciences, GJU S&T, Hisar
2
Institute of Pharmaceutical Sciences, K.U. Kurukshetra
E-mail- deepika.saini.666@gmail.com

Abstract
Resistance of malaria parasites has quickly developed to almost all used antimalarial drugs.
Accordingly,the discovery of new effective drugs to counter the spread of malaria parasites that
are resistant to existing agents, especially acting on multi-targets, is an urgent need. The cysteine
protease falcipain-2 (FP-2)and dihydrofolatereductase (DHFR) play crucial roles in the
Plasmodium life cycle. In this study, a seriesof quinolinylpyrazoles as dual inhibitor of FP-2 and
DHFR have been designed using Molecular modelling techniques like docking simulations,
pharmacophore modelling etc. with the aid of Autodockvina and LigandScout.
Quinolinylpyrazoles were synthesised by VilsmeierHaack reaction and further characterized by
IR and NMR spectroscopy. Compound 4d (4-Cl substituted) was found to possess best dual
activity against both the receptors in docking study.

PCHEM-17

THE DISCOVERY OF LESS TOXIC NOVEL DPP-IV INHIBITORS:
PHARMACOPHORE MODELING, VIRTUAL SCREENING, MOLECULAR
DOCKING AND I N SI LI CO ADMET STUDIES

Ajay Kumar, Aman Thakur*, Dhirender Kaushik
Email: amanthakur5052@gmail.com

Abstract
The identification of important 3D spatial chemical features of DPP-IV inhibitors will be helpful
to discover the potential of anti-diabetic activity. The best ligand based pharmacophore
hypothesis, one hydrogen bond donor (HBD), two hydrogen bond acceptor (HBA), two positive
ionizable areas (PIA) and one hydrophobic region (HY) and structure based pharmacophore
model one HBD, one HBA, one PIA and one HY region were generated using LigandScout. Test
and Decoy sets were used for the validation of generated pharmacophore models and used
further for virtual screening of the Zinc library. Subsequently, molecular docking and ADMET
studies were employed to evaluate the drug-likeness properties of the hit compounds. Finally,
twelve compounds were obtained as novel leads to inhibit the DPP-IV for anti-diabetic activity.

August 22, 2014


PCHEM-18

Molecular Modeling and ADMET Prediction Studies of Novel Butenolides as Potential
Inhibitor of Plasmodium falciparumL-Lactate Dehydrogenase

DeepikaChoudhary ,SukhbirLalKhokra
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana

Abstract
Malaria, in particular that caused by Plasmodium falciparum, is prevalent across the tropics, and
its medicinal control is limited by widespread drug resistance. Complications associated with
drug resistance necessitate the discovery of effective new anti-malarial agents. L-Lactate
dehydrogenase of Plasmodium falciparum reveals a new potential target for anti-malarial design.
In present study, a combined approach of virtual screening including docking study and ADMET
prediction is applied to evaluate a set of designed hypothetical butenolides for anti-malarial
potential. Docking studies were performed against an X-ray crystal structure of Plasmodium
falciparum L-Lactate dehydrogenase (PDB ID- 1LDG), using Glide docking program. Poses
were evaluated by counting the no. of favorable interactions, glide score and glidemodel score.
Out of eighty eight hypothetical compounds, twenty eight compounds (1k, 2d, 2i, 2j,4d, 5b, 5d,
6a, 6b, 6c, 6d, 6e, 6i, 6j, 7a, 7b, 7c, 7d, 7e, 7f, 7h, 7i, 8i, 8b, 8c, 8d, 8i) were having better glide
score and ten compounds (4a, 4b, 4c, 4e, 4h, 4i, 4k, 5d, 6j, 8e) shown good no. of interactions as
compared to the standard. Thereafter, all designed butenolides were filtered using in silico
calculation of physiochemical properties by software QikProp 3.6, such as molarweight (MW),
number of rotatable bonds (NRB), lipophilicityparameter (log p), number of hydrogen bond
acceptors(HBA), number of hydrogen bond donors (HBD), solubility (log s), percentage human
oral absorption, Lipinski violations etc. Twenty four analogues (1a, 1b, 1c, 1d, 1e, 1f, 1g, 1h, 1i,
1j, 2d, 3c, 3d, 3i, 3j, 4a, 4c, 4d, 4i, 4j, 5i, 8c, 8d, 8j) lied in the specified range of the
pharmacokinetic properties. Results of the in silico virtual screening and docking studies
revealed that compounds 2d, 4a, 4c, 4d, 4i, 8c and 8d have good binding affinity as well as
ADMET properties to become successful drugs.

PCHEM-19

SYNTHESIS, ANTIMICROBIAL AND ANTICANCER EVALUATION OF N-[2-(4-
CHLORO-PHENYL)-4-OXO-4H-QUINAZOLIN-3-YL]-2-{4-[2-(SUBSTITUTED
PHENYLAMINO)-ACETYL]-PIPERAZIN-1-YL}-ACETAMIDE DERIVATIVES

Sanjiv Kumar, Shinky Mehta, Pradeep Kumar, Rakesh K. Marwaha and
BalasubramanianNarasimhan
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak 124001, India.
E-mail ID: sanjiv.pharmchem@gmail.com, naru2000us@yahoo.com

August 22, 2014


Abstract
In the present study, a series of N-[2-(4-chloro-phenyl)-4-oxo-4H-quinazolin-3-yl]-2-{4-[2-
(substituted phenylamino)-acetyl]-piperazin-1-yl}-acetamide derivatives (1-17) were synthesized
and characterized by physicochemical means. The synthesized compounds were screened for
their antimicrobial and anticancer potentials. The in vitro antimicrobial activity results indicated
that the synthesized compounds were more active against fungal strains as compared to bacterial
strains. 2-{4-[2-(4-amino-2-methyl-phenylamino)-acetyl]-piperazin-1-yl}-N-[2-(4-chloro-
phenyl)-4-oxo-4H-quinazolin-3-yl]-acetamide (3) was found to be the most potent antifungal
agent, having more antifungal activity than standard drug, fluconazole. The in vitro anticancer
activity results indicated that N-[2-(4-chloro-phenyl)-4-oxo-4H-quinazolin-3-yl]-2-{4-[2-(2,3-
dichloro-phenylamino)-acetyl]-piperazin-1-yl} acetamide (5) (having anticancer activity
comparable to standard drug 5-fluorouracil (5-FU)) was found to be the most active anticancer
agents

PCHEM-20

DESIGN, SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 1-AMINO-1, 8a-
DIHYDROQUINOLINE-2(4ah)-ONE DERIVATIVE

Savitri ,Sanjiv Kumar, Rakesh K. MarwahaandBalasubramanianNarasimhan
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak-124001
E-mailID: savitribharti@gmail.com, naru2000us@yahoo.com

Abstract
Antibiotic resistance is recognized as a major global health security issue that threatens a return
to the pre-antibiotic era, with potentially catastrophic economic, social and political
ramifications. Coumarins have attracted intense interest in recent years because of their diverse
pharmacological properties such as anticancer, antibacterial, antiviral,
anti-inflammatory, antidepressant and antitumor potentials. In the present study, a series of
1-amino-1,8a-dihydroquinoline-2(4aH)-one derivatives were synthesized and characterized by
physiochemical means.All the newly synthesized compounds were investigated for their
in vitro antimicrobial potential against Gram positive bacteria (S. aureus and B. subtilis), Gram
negative bacterium (E. coli) and fungal strains (C. albicans and A. niger) by tube dilution
method. Results of antimicrobial study indicated thatcompound 13 (MIC
sa
= 1.68 x 10
-2
M/ml
and compound 20 MIC
ca
= 1.01 x 10
-2
M/ml) were found to be most potent antibacterial and
antifungal agent comparable to norfloxacin and fluconazole against
S. aureusas well as C. albicans, respectively and may be taken as lead compound for the
development of novel antimicrobial agents.

August 22, 2014


PCHEM-21

DESIGN, SYNTHESIS AND ANTIMICROBIAL EVALUATION OF
N-SUBSTITUED-2-(1H-BENZOTRIAZOL-1-YL)ACETOHYDRAZIDEDERIVATIVES

Manisha,SaloniKakkarandBalasubramanianNarasimhan
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak 124001, India.
E-mail ID: nimbhal2111@gmail.com, naru2000us@yahoo.com

Abstract
Although number of drugs is available in the market, but the need of discovering the new
antimicrobial drugs with better pharmacokinetic profile and lesser toxicity has become the main
objective in the field of medicinal chemistry, it is also due to the fast microbial resistance to the
existing molecules. Paradoxically, some pharmaceutical companies have in dicated that they are
curtailing anti-infective research program. Heterocyclic compounds containing nitrogen atoms
are considered to be one of the most effective antimicrobial drugs used as either single agents or
in combination for cancer therapy. 1,2,3-benzotriazole derivatives became the most important
compounds with the advantage of low toxicity, high oral bioavailability and broad spectrum
activity. A series of N-substitued-2-(1H-benzotriazol-1-yl) acetohydrazidederivativeswas
synthesized and evaluated for its antimicrobial potentials.Results of antimicrobial study indicated
thatcompound 5 (MIC
ec
= 0.81x 10
-2
M/ml) was found to be most potent antibacterial agent.
Antifungal activity results indicated that compounds 11, 17 (MIC
an
= 0.96 x 10
-2
M/ml and
MIC
ca
= 1.60 x 10
-2
M/ml) were found to be most potent antifungal agents against A. nigerand
C. albicans respectively and may be taken as lead compound for the development of novel
antimicrobial agents

PCHEM-22

Thiazolidinones: the versatile pharmacophore of medicinal significance

Sonakshi Seth*, SukhbirLal
Laureate Institute of Pharmacy, Jawalaji, Himachal Pradesh

Abstract
A wide variety of heterocyclic systems have been explored for developing pharmaceutically
important molecules. 2,4-Thiazolidinone and its derivatives represented the most promising
group of compounds having a variety of pharmacological features. Owing to the wide range of
pharmacological activities, the synthesis of compounds having Thiazolidine-4-one moiety has
become an important target during last few decades as they are endowed with variety of
biological activities and have wide range of therapeutic properties. Thiazolidine-4-one has been
reported to possess antibacterial, antifungal, antiviral, analgesic, anti-inflammatory, anti-
tubercular, anti-oxidant, anti-cancer and anti-convulsant activities. A number of Marketed
compounds possessing thiazolidinone ring are Ralitoline(Anticonvulsant),
August 22, 2014


Spiclomazine(Psychotropic),Piprozolin(Choleretic),Etozolin(Antihypertensive),Mezolidon(Antiu
lcer),Nitrodan(Antihelmintic), Epatrestat(Aldose reductaseinhibtor).Traditionally, searching for
lead drug compound was done by screening existing synthetic compound but now days,
combinatorial chemistry has evolved as an emerging technology with the potential to rapidly
expand aroundidentified hits for lead progression.Combinatorial chemistry and multiple parallel
synthesis have transformed the eld of medicinal chemistry for the better. It helps not only in
optimizing the activity but also aid in molecular recognition and in understanding the mechanism
of action.The anti-cancer and anti-HIV activities are the most encouraging activities for the
pharmacists so by the present scenario it can be concluded that 2,4- thiazolidinones have a great
potential which remain to be disclosed till date.

PCHEM-23

SYNTHESIS OF NOVEL IMIDAZOLE CLUBBED TRIAZOLE DERIVATIVES

Archana Sharma
1
, Vipin Kumar
2
, Sunil Kumar
1
, Dharam Pal Pathak
3
1
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119, India
2
School of Chemical Sciences and Pharmacy, Central University of Rajasthan, Bandarsindri, Kishangarh-
305801 India
3
Delhi Institute of Pharmaceutical Sciences and Research, New Delhi-110017, India
E-mail: archanakuk@gmail.com

Abstract
The imidazole scaffold is extremely versatile and has been featured in a number of clinically
used drugs. The triazole scaffold is also known as biologically active compound present in
number of drugs. During recent years, there has been a large investigation on different classess
of substituted imidazole bearing triazole compounds. Many of the compounds have been found
to possess an extensive spectrum pharmacological activities such as antibacterial and antifungal,
analgesic and antiinflammatory, anticancer, antitubercular, antiviral, antimalarial, antidiabetic
etc. The present work includes synthesis of five new imidazole clubbed triazole derivatives.
Compounds have been synthesized employing benzil as a starting material which was reacted
with different aromatic aldehydes, ammonium acetate and sulphanilic acid catalyst in basic
medium composed of sodium bicarbonate and N,N-dimethylformamide to yield 2,4,5-
trisubstituted imidazoles. The imidazoles were then reacted with 3-amino-1,2,4-triazole and
carbon disulphide to yield imidazole bearing triazole derivatives. The chemical structures of all
the synthesized compounds were confirmed by IR and
1
HNMR spectral data.

August 22, 2014


PCHEM-24

SYNTHESIS AND ANTIMICROBIAL EVALUATION OF AMINO-BIPHENYL-3-
CARBALDEHYDE DERIVATIVES

Ritika Bhatia. Sanjiv Kumar. BalasubramanianNarasimhan*
Faculty of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak-124001, India.
*Corresponding author E-mail: naru2000us@yahoo.com

Abstract
Multidrug resistance is a condition which enables the disease causing microorganisms to resist
distinct antimicrobial viz. antibiotic, antifungal, antiviral, and anti-parasitic drugs. This creates a
serious problem as the emergence and spread multi drug resistant gram positive bacteria such as
methicillin resistant Staphylococcus aureus (MRSA), penicillin resistant
Streptococcuspneumonia (PRSP), and vancomycin resistant enterococci (VRE) have made
treatment of infectious diseases difficult.The current scenario highlights the need for the
discovery and development of new lead compounds of simple structure, exhibiting optimal
antimicrobial potency and new mechanisms of action with minimum side effects. The search for
new drugs which can selectively target the tumour cells is todays goal of chemotherapy and is a
never ending process, till the goal is reached.Benzylidene aniline derivatives are a very important
class of pharmacologically active compounds. They have been extensively studies and are
proved to possess a plethora of pharmacological activity such as antimicrobial, anticancer, anti-
inflammatory, antioxidant, anti-mycobacterial, antimelanogenesis activities.A series of amino-
biphenyl-3-carbaldehyde (benzylidene aniline) derivatives were synthesized (1-11) and were
evaluated for its antimicrobial potential. All the synthesised compounds showed comparative
antimicrobial properties,however compound 6 (MIC=3.44 x 10
-2
M/ml) and compound 1
(MIC=4.38 x 10
-2
M/ml) were found to be most potent antibacterial and antifungal respectively.

PCHEM-25

QUINOLINE AS ANTIMALARIAL AGENTS: A REVIEW

Ajay Kumar, Manju Sharma*
Institute of Pharmaceutical Sciences, K.U. Kurukshetra
E-mail-sharmamanju134@gmail.com

Abstract
Malaria, a life threatening parasitic disease, which mainly thrives in the underdeveloped and
developing countries, is a major issue of research for its eradication.As per WHO report,
approximately 3.3 billion people across the world remain susceptible to this lethal disease.
Quinoline, a basic antimalarial pharmacophore, has been modified to a great extent which has
now led to the development of diverse antimalarial drugs such as mefloquine, primaquine,
ferrocene, amidiaquine etc. The present review comprises of the important aspects of these
August 22, 2014


structural modifications including the study of various series like 4-aminoquinolines, 8-
aminoquinolines, quininylchalcones, pyrimidinylquinolines, bisquinolines, metal based
quinolines, anilinoquinolines and quinolines from nature along with their reported potent
antimalarial agents. This work also presents the future strategies for chemotherapy of malaria,
which may open up a new gateway for further research.

PCHEM-26

PYRAZOLE AND ITS DERIVATIVES OF ANTI-DIABETIC POTENTIAL: A REVIEW

Ajay Kumar, Usha Rani*
Institute of Pharmaceutical sciences, Kurukshetra
e-mail: urani3117@gmail.com

Abstract
Pyrazole is 5-membered aromatic ring, containing three carbon atoms and two nitrogen atoms at
adjacent positions. Studies showed that these compounds have antitumor, antibacterial,
antifungal, antiviral, anti-parasitic, anti-tubercular, anesthetic, anti-diabetic, analgesic and potent
selective activity such as Nitric oxide synthase (NOS) inhibitor and Cannabinoid CB1 receptor
antagonist activity. Due to wide diversity of pharmacological activity, pyrazole derivatives are of
keen interest for the medicinal chemists. Current study is focused on the pyrazole and
structurally related heterocyclic of anti-diabetic interest. From the literature, pyrazole has been
proved to be a successful candidate possessing anti-diabetic potential by in-vitro and in-vivo
pharmacological models. Current review includes ample study of synthetic procedures along
with the anti-diabetic potential of the pyrazoleanalogs.

PCHEM-27

PYRIDOACRIDINES AS NATURAL ANTICANCER LEAD COMPOUNDS: A REVIEW

Vikas Sharma
1
*, Vipin Kumar
1,2
, PrabodhChander Sharma
1

1
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana-136118
2
Department of Pharmacy, School of Chemical Sciences and Pharmacy, Central University of
Rajasthan, Ajmer-305801, Rajasthan.

Abstract
Pyridoacridine alkaloids are natural products, isolated from different biological sources like
sponges, ascidians, mollusk, coelenterate etc. The first pyridoacridine i.e.amphimedine, was
reported as a cytotoxic component of the sponge Amphimedon. Since 1983, over 50
pyridoacridines like sampaginine, eilatin, kuanoniamine,ascidideminehave been discovered and
their pharmacological activities have been studied. Almost all pyridoacridines reported are
cytotoxic to mammalian cells in culture. Pyridoacridine from a variety of sources have the ability
August 22, 2014


to display different biological activities viz. anti-viral (e.g. dercitin), fungicidal (e.g. meridine),
anti-protozoaletc. Furthermore, from these natural pyridoacidines; researchers around the world
are synthesizing medicinally active synthetic derivatives; some of them displayed activity better
than well known standard compounds. For example: Kuanoniamine analogues displayed anti-
protozoal activity better than standard compounds i.e. pentamidine, pyrimethamine,
sulfadiazine and spiramycin.So, as pyridoacridines and its analogues displaying different
activities therefore they can be considered as ProspectiveLead compounds of future.
Structural activity relationship points will further prove their candidature as lead compounds for
different ailments.

PCHEM-28

Synthesis and discovery of novel Dispiro compounds as inhibitors of
acetylcholinesterase.

PankajSaraswat*, Dr. G. Jeyabalan, Gurpreet Singh
New Drug Discovery Research, Department of Medicinal Chemistry, Alwar Pharmacy College,
Alwar 301030, Rajasthan, India
Email- pankaj.mph09@gmail.com

Abstract
In present investigation, a series of spiro-[2.3'']-oxindole-spiro[3.3]-1-Ethylphenyl-5-
(substitutedarylmethylene)-tetrahydro-4-(1H)-pyridinone-4-arylhexahydro-1H-
pyrrolizineanalogues were synthesized and were evaluated for their inhibitory activities toward
AChE(Acetylcholinesterase) in vitro. Among the synthesized compounds, compound spiro-
[2.3'']-5-nitro-oxindole-spiro[3.3']-1'-Ethylphenyl-5'-(4-piperidine-1-yl-arylidine)-tetrahydro-4'-
(1H)-pyridinone-4-(4-piperidine-phenyl)hexahydro-(1H)-pyrrolizine(C2) produced significant
activities with 0.09 0.2 mol/L.

PCHEM-29

Synthesis and Biological Evaluation of Pyrimidine Analogues as
Anticonvulsant Agents

Mohd.ZaheenHassan
a
, Mohammad Amir
b
, G. Jeyabalan
a
, and NarendraNyola
a

a
Department of Pharmaceutical Chemistry, Alwar Pharmacy College, Alwar, Rajasthan-301030
b
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hamdard University, New Delhi-110
062, India.

Abstract
Epilepsy is a serious chronic CNS disorder characterized by the periodic and unpredictable
occurrence of seizures arising due to paroxysmal abnormality of brain functions affecting around
August 22, 2014


50 million people globally. Around 30% epileptic patients are pharmacoresistant to the newer
AEDs, therefore the development of novel anticonvulsants with safer therapeutic index will
broaden treatment options for epileptic patients. Pyrimidine nucleus represents a potential moiety
possessing diverse pharmacological properties like antihypertensive, antiviral, anticancer and
anticonvulsants. Recently, some pyrimidine derivatives have also been reported to have good
anticonvulsant activity against the various seizure models. Encouraged by these observations and
in continuation of our ongoing research program on anticonvulsants, herein we report the
synthesis of some new pyrimidine derivatives. The synthesized compounds were found to have
significant anticonvulsant and GABAergic activity without any neurotoxicity. The titled
compounds were also devoid of any CNS depressant effects.

PCHEM-30

DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF NEWER PYRAZOLINE
ANALOGUE

Veerendra Saini
Department of Pharmaceutical Chemistry, Maharishi Arvind College of Pharmacy, Ambabari,
Jaipur, Rajasthan

Abstract:-
Medicinal chemistry and pharmaceutical chemistry are disciplines at the intersection
of chemistry, especially synthetic organic chemistry, and pharmacology and various other
biological specialties, where they are involved with design, chemical synthesis and development
for market of pharmaceutical agents, or bio-active molecules (drugs). The main aim is to design
and synthesize newer Pyrazoline derivatives and evaluate these for Anti- Tubercular activity.
The literature reveals that pyrazolines have also shown a wide range of pharmacological and
biological activities as well as they have been found to possess antiproliferative , antimalarial,
antibacterial and antitubercular activity.Thus, activity profile inspired us to synthesize new
molecular frame work of pyrazoline derivatives with the objective of minimizing the adverse
effects of current drugs. Synthesized intermediate and final molecules will be characterized using
IR,
1
H NMR and mass spectroscopy. By IR spectral analysis all synthesized molecules will be
characterized to determine the characteristic functional group in the molecule. By
1
H NMR
spectral analysis one of the representative molecule will be characterized to determine the
number & type of hydrogen atoms in the molecule. By Mass spectral analysis molecule will be
characterized to determine peak and molecular fragmentation.

August 22, 2014


PCHEM-31

DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF NEWER PYRAZOLE
ANALOGUE

Kavita choudhary
Department of Pharmaceutical Chemistry, Maharishi Arvind College of Pharmacy, Ambabari,
Jaipur, Rajasthan

Abstract
Medicinal chemistry is the chemistry discipline concerned with the design, development and
synthesis of pharmaceutical drugs. The discipline combines expertise from chemistry and
pharmacology to identify, develop and synthesize chemical agents that have a therapeutic use
and to evaluate the properties of existing drugs. The main aim is to design and synthesize novel
pyrazole analogues and evaluate them for anti-cancer activity. pyrazole and its analogues have
emerged with various biological activities such as anticancer, antibacterial, antioxidant,
antimalarial, anti-mitotic,anti-inflammatory activities. Thus, pyrazole analogues afford us to
synthesize newer pyrazole derivatives with minimal adverse effects of current anti-cancer drugs,
targeting cancerous cells with minimum effect on normal cell lines and to decrease the
development of resistance caused due to anti-cancer agents. Synthesized intermediate and final
molecules will be characterized using IR,
1
H NMR and mass spectroscopy. By IR spectral
analysis all synthesized molecules will be characterized to determine the characteristic functional
group in the molecule. By
1
H NMR spectral analysis one of the representative molecule will be
characterized to determine the number & type of hydrogen atoms in the molecule. By Mass
spectral analysis molecule will be characterized to determine peak and molecular fragmentation.

August 22, 2014


PHARMACOGNOSY
ABSTRACTS

August 22, 2014


PCOG-1

STUDIES OF ANXIOLYTIC EFFECTS OF AN AYURVEDIC NANOMEDICINE AKIK
BHASMA

Vijender Kumar, Gaurav Gupta*, Sandeep Kumar Goyal, Arun Kaura, V. Gupta
University Institute of Pharmaceutical Sciences & Research, BFUHS, Faridkot-151203

Abstract
Anxiety is one of most common CNS related disorders affecting about 450 million people
worldwide. Furthermore, it is difficult to predict which patient will respond to any given
treatment. In the traditional systems of medicine, many formulations have been used to treat
anxiety like disorders for thousands of years. The present study was designed to first time
evaluate the antianxiety effects of the Akik bhasma (50mg/kg and 100mg/kg) and compared with
diazepam (2mg/kg) as standard, further with untreated control group of experimental induced
anxiety in rats. Antianxiety activity was tested using different methods like elevated plus maze
(EPM) and light-dark test (LDT). The result indicate that Akik bhasma 100mg/kg most
significantly (p< 0.01) increase the no. of entry and time in EPM, whereas increase time in light
box in LDT with reference to standard drug. Present research work was concluded that Akik
bhasma have anxiolytic potential.

PCOG-2

EFFECT OF SUCCESSIVE LEAF EXTRACTS OF BASELLA ALBA IN GASTRIC
ULCER

Vijender Kumar, Lovedeep Kumar*, Sandeep Kumar Goyal, Arun Kaura
University Institute of Pharmaceutical Sciences & Research, BFUHS, Faridkot-151203

Abstract
Successive leaf extracts of Basella alba var. alba were investigated for antiulcer activity on rats
employing the aspirin induced ulcer and pylorus ligation models. The various biochemical
parameters such as total acidity, free acidity, gastric acid volume, p
H
, ulcer index, percent
protection and histopathological sections were comparatively examined between control, test and
standard groups (n=6). Predetermined selected bioactive extracts like ethyl acetate extract of
Basella alba (EEBA) and methanol extract of Basella alba (MEBA) were studied in rats. This
study was evaluated with the doses of 1ml/kg of absolute ethanol, 50 mg and 100 mg of each test
extracts of individually and 20 mg/kg of ranitidine for control test and standard groups
respectively. Histopathological and pre-treated with EEBA rats showed most significant (p<0.5)
reduction in gastric mucosal damage as compared to control and MEBA. The results of this study
indicated that Basella alba have antisecretory and gastro protective effect.

August 22, 2014


PCOG-3

MICROWAVE- ASSISTED EXTRACTION: NOVEL TECHNIQUE FOR ISOLATION
AND EXTRACTION OF PHYTOCONSTITUENTS

Dhruv Sabharwal*, Reecha Madaan, Sandeep arora
Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab

Abstract
Microwave-assisted extraction (MAE) is widely employed in the analysis and extraction of
active compound from plant. MAE offers a rapid delivery of energy to total volume of solvent
and solid plant matrix with the subsequent heating of solvent and solid matrix, efficiently and
homogeneously. The effect of microwave energy is strongly dependent on nature of both solvent
and solid matrix. The main advantage of MAE over the conventional extraction is that it reduces
solvent consumption, it has shorter operational time, it posses moderately high recoveries, has a
good reproducibility and minimal sample manipulation for extraction process. MAE is also
comparable to other modern extraction techniques such as supercritical fluid extraction due to its
process simplicity and low cost. MAE can be practised in two different modes- one is closed
vessel operation that is controlled elevated pressure and temperature, another is open vessel
operation performed at atmospheric pressure, named as pressurized microwave-assisted
extraction (PMAE) and focused microwave-assisted extraction (FMAE) respectively. Solvent
free MAE (SFMAE) has been designed, where the moisture content within the plant matrix itself
serves extraction and no solvent are used. Various modified MAE such as vacuum microwave
assisted extraction (VMAE), nitrogen protected microwave- assisted extraction (NPMAE)
,ultrasonic microwave-assisted extraction (UMAE) ,dynamic microwave-assisted extraction
(DMAE) are also employed.

PCOG-4

FLORASOLS/PHYTONIC EXTRACTION: AN ADVANCED TECHNIQUE.

Jaskaran Singh*, Reecha Madaan, Paramdeep singh
Chitkara College of Pharmacy, Chitkara University, Rajpura.

ABSTRACT
The florasols or phytonic extraction is one of the newest extraction method using 1,1,2,2-
tetrafluroethane better known as hydro fluorocarbon- 134a (HFC-134a). It is perhaps one of the
best extraction methods due to the amount of product produced and high purity of the oils
produced by this method. It is much gentler and feasible than CO
2
extraction, another advantage
is that it is performed under low temperatures thus prevent degradation of oils.
It is used for extracting an organic component, including fragrant, flavorsome or
pharmacologically active components, from an organic material of natural origin using
tetrafluoroethane in the liquid phase as the solvent. The process essentially includes contacting
the organic material with the tetrafluoroethane in its liquid phase, charging thereby the
August 22, 2014


tetrafluoroethane with the organic component, collecting the mixture thereof, and removing the
tetrafluoroethane there from so as to isolate the organic component.

PCOG-5

SEPERATION AND QUALITATIVE DETERMINATION OF FLAVONOIDS FROM
TETLEY TEA

Andleeb kaur*, Jasmina Kapur, Surya Karan Seth, Vaishali Arora, Paramdeep Singh
Chitkara College of Pharmacy, Chitkara University, Chandigarh-Patiala National Highway,
Rajpura 140401, Patiala, Punjab, India.
E. Mail: paramdeepsond87@gmail.com,

Abstract
Tetley is one of the biggest-selling tea brands in the western world. It is biggest-selling brand in
Canada and the second biggest-selling in the United Kingdom and the United States. The
company claims the presence of antioxidants and flavonoids in tea. The present study aims to
develop a novel technique for separation of flavonoids and check the presence of flavonoids
qualitatively using various chemical tests, including thin layer chromatography (TLC) in Tetley
tea. Firstly, methanolic extract of the tea was prepared using soxhlet apparatus and then
concentrated extract was consecutively extracted with petroleum ether, chloroform and ethyl
acetate. Alkali test, ferric chloride test, lead acetate test, zinc hydrochloric acid reduction test,
shinoda test and thin layer chromatography were employed in the study which showed the
presence of flavonoids in ethyl acetate fraction. The present study suggested above mentioned
separating procedure and ethyl acetate fraction for the separation of flavonoids and confirms the
presence of flavonoids in Tetley tea. More research is needed to confirm proclaimed claims by
various food and beverage industries for their products.

PCOG-6

MAHANIMBIN: ANTIANXIETY AND ANTIDEPRESSANT CONSTITUENT OF
MURRAYA KOENI GI I L. LEAVES

Jyoti Dahiya, Jitender Singh, Sonali Batra* and Anupam Sharma
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160 014

Abstract
Previous studies have revealed significant antianxiety and antidepressant activities of
hydroalcoholic extract of M. koenigii leaves at 500 mg/kg. Present study was undertaken to
isolate antianxiety and antidepressant constituent of M. koenigii leaves. The ethyl acetate fraction
exhibited significant anxiolytic and antidepressant activities at the dose of 125 mg/kg, p.o. using
elevated plus-maze and Porsolts despair swim test respectively. Column chromatography of
bioactive ethyl acetate fraction yielded 5 sub-fractions (F
1
-F
5
). F
2
showed significant antianxiety
August 22, 2014


and antidepressant activities at the dose of 3 mg/kg p.o. The activities were comparable to the
standard drugs diazepam (2 mg/kg) and imipramine (10 mg/kg). A compound was separated
from the F
2
fraction, and structure was elucidated by IR and NMR analysis. The compound was
characterized as Mahanimbin. The content of Mahanimbin was estimated in M. koenigii leaves
by a validated TLC densitometric method, and found to be 0.104% w/w.
Keywords: Murraya koenigii, antianxiety, antidepressant, Mahanimbin.

PCOG-7

COMPARATIVE ANTIANXIETY EVALUATION OF ARGYREI A SPECI OSA LINN.
(ROOTS), CAESALPI NI A DI GYNA ROTTLER (ROOTS) AND SPHAERANTHUS
I NDI CUS LINN. (FLOWERS)

Jitender Singh*, Ashwani Kumar, Anupam Sharma
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014

ABSTRACT
Aim of the present study was to evaluate the antianxiety activity of various extracts (petroleum
ether, chloroform, ethanol and water) of three traditionally used plants viz. Argyreia speciosa
(roots), Caesalpinia digyna (roots) and Sphaeranthus indicus (flowers). All biological studies
were conducted on laca mice, and the test materials were administered per oral route. Acute oral
toxicity studies were conducted using OECD guidelines 423. Results indicate that all the extracts
were safe upto 2000 mg/kg. The present study revealed that out of all the 12 extracts, ethanol
extract of C. digyna showed maximum anxiolytic effect at 400 mg/kg on elevated plus-maze, and
the results were comparable to the standard anxiolytic drug diazepam (2 mg/kg). Phytochemical
investigation of the most active extract, i.e., ethanol extract of C. digyna roots revealed the
presence of phenols, phytosterols, terpenoids, tannins, amino acids and carbohydrates.

PCOG-8

IONIC LIQUIDS BASED MICROWAVE ASSISTED EXTRACTION- THE FUTURE OF
HERBAL EXTRACTION

Meenu Bhan*, Munish Garg
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001

Abstract
Ionic liquids are room temperature or near room temperature liquids composed entirely of ions.
They have unique physical properties like low melting point, negligible vapour pressure,
excellent thermal stability, high conductivity, reusability and are ecofriendly in nature. These are
also called as green solvents because they are the source of green extraction as they do not
evaporate volatile organic compounds (VOC) which are hazardous in nature. Ionic liquids when
coupled with recent extraction techniques, especially Microwave Assisted Extraction (MAE)
August 22, 2014


technique gives much better results with high percentage yield as reviewed from the literature.
This is due to the best absorbing capacity of microwaves by the ionic liquids and the structural
changes occurred in cell wall of the herbal drugs. Present work explains the significance and
future prospects of this novel extraction technique.

PCOG-9

MEDICINAL POTENTIAL OF THUJ A ORI ENTALI S (LINN.) FRANCHO

Preeti bansal and Sumitra singh
Technology, Hisar-125001, Haryana, India

Abstract
Thuja orientalis (Arbor Vitae means tree of life, Family- Cupressaceae) also known as
morpankh in Hindi. It is an evergreen, graceful and highly aromatic narrow pyramidal tree or
shrub that is widely cultivated in gardens of temperate and semi-temperate areas. It is used in
various forms of traditional medicines. Traditionally plant has been used internally in the
treatment of coughs, haemorrhages, excessive menstruation, delayed menstruation bronchitis,
asthma, skin infections, mumps, bacterial dysentery, arthritic pains and premature blandness,
enuresis, cystitis, psoriasis and uterine carcinomas. In Chinese system of medicine the plant is
used as anticancer, anti-haemorrhagic, tonic, dysentry, hair loss, expectorant, antitussive,
insomnia and for gout treatment. The plant is rich in pharmacologically active phytoconstituents
such as flavonoids, tannin, essential oils, lignans, phlobaphenes, bisnorlabdane and
trisnorlabdane type mono and diterpenoids etc. Essential oil in the plant is a complex blend of
sesquiterpene hydrocarbons (cuparenes), alcohols (cedrol, widrol, cuparenols), monoterpenic
acid and ketones. This plant oil has tremendous pharmacological activites such as antibacterial,
antifungal, antiviral, larvicidal and nematicidal activity against different pests. The reported
biological property are antiphlogistic and antiallergic, platelet activating factor inhibition,
antielastase activities and anticancer. Thuja orientalis ointment preparations can be efficiently
used against microbial/worm infection. This plant has a lot of medicinal potential and has a
incredible future for research and formulation development.

PCOG-10

A Medicinal Mushroom Phellinus An Overview

Uzma Azeem*
1
, Gurpaul Singh Dhingra
1
and Richa Shri
2
1
Department of Botany, Punjabi University, Patiala,

2
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-
147002.
Presenting author*: uzmaazeem2@gmail.com

Abstract
August 22, 2014


The genus Phellinus was established by Qulet in 1886 with the type species Phellinus igniarius.
It is one of the largest basidiomycete genus belonging to family Hymenochaetaceae, order
Hymenochaetales class Agaricomycetes, sub-phylum Agaricomycotina and phylum
Basidiomycotina. The genus is characterized by resupinate to pileate, single or imbricate
basidiocarp, brownish hymenial surface with isodiametric pores; yellowish, rusty brown, grey to
black tomentose, hispid, glabrous or deeply cracked abhymenial surface; dimitic hyphal system
with simple septate generative hypahae and thck walled skeletal hyphae; presence of or rarely
absence of setae, four spored basidia and globose to cylindrical, smooth hyaline to rusty brown,
thin to thick walled, dextrinoid to inamyloid basidiospores. According to the work of Kirk et al.,
2008, there are 180 species of Phellinus known worldwide. The studies done by various workers
from time to time in India 86 species of Phellinus have been reported so far. These days various
species of Phellinus have been receiving a great attention because of their effective role on a
diversity of diseases. Most of the species are lignicolous causing white rot. Various Phellinus
species have been recognised as therapeutic agents in traditional Chinese medicine and have
been used traditionally for the treatment of stomachaches, inflammation, arithritis,
hepatoprotective, enhancing detoxification, combating allergy and diabetes, improving blood
circulation, gastroenteric disorders, tumors and lymphatic disorders. Many species of Phellinus
have been investigated for their chemical constituents and therapeutic activities. Some species of
Phellinus are found to exhibit antitumor, antioxidant, antiarithritic, antidiabetic and antimicrobial
activities. All these effects of Phellinus species are attributed to various bioactive constituents
isolated from them such as polysaccharides, hispidin, -glucans, Pyrano[4,3c][2]benzopyram-
1,6-dione derivative, phelligridime and proteoglycans. However, the genus needs much scientific
work to explore its therapeutic and medicinal uses so that the fungus can be used in food and
drug formulations.

PCOG-11

MALTOL: THE ANALGESIC CONSTITUENT OF ABI ES PI NDROW ROYLE

Deepak Kumar
*
, Suresh Kumar
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147002,
Punjab, India

ABSTRACT
Abies pindrow Royle (Himalayan Silver Fir; family Pinaceae) has been traditionally used as
analgesic, anxiolytic, antidiabetic and anti-inflammatory drug. Despite a long tradition of use, the
plant has not been systematically evaluated for analgesic activity. Thus, it was planned to isolate
analgesic constituent(s) from A. pindrow aerial parts using bioactivity-guided fractionation.
Properly identified A. pindrow aerial parts were successively extracted using solvents in
increasing order of polarity viz., petroleum ether (60-80C), chloroform, methanol and water. All
extracts were evaluated for analgesic activity at the doses of 200 or 400 mg/kg, p.o. in mice
using tail immersion test. The efficacy of A. pindrow was statistically compared with the
standard analgesic drug, morphine (5 mg/kg, i.p.). Among various extract of A. pindrow aerial
parts, only chloroform extract exhibited significant analgesic activity equivalent to the standard
August 22, 2014


drug. Column chromatography of chloroform extract yielded six fractions (F
1
-F
6
). F
2
and F
3
were
found to possess significant analgesic activity. Column chromatography of F
2
and F
3
led to
isolation of two constituents (DS-1 and DS-2). Further, DS-1 exhibited significant analgesic
activity, and its structure was elucidated as maltol. Traditional claims of A. pindrow aerial parts
for analgesic activity are validated, and the activity is attributed to maltol, which was isolated
from plant following bioactivity-guided fractionation. It is concluded that maltol is responsible
for analgesic effects of this traditionally used plant.

PCOG-12

PHARMACOGNOSTIC INVESTIGATIONS AND PHARMACOLOGICAL
EVALUATION OF ALSTONI A SCHOLARIS R. BR. LEAVES IN NEUROPATHIC PAIN

Hasandeep Singh* and Balbir Singh
Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar-143005 Punjab
email- balbir_gndu@yahoo.com

Abstract
Authentication of plant material is prerequisite before using it as research material. Therefore it
was planned to establish pharmacognostic standards. A. scholaris was subjected to macroscopic
and microscopic studies. The color of leaf is green and the leaf blade is elliptic-lanceolate or
elliptic-obovate. Lateral veins are about 25-40 on each side of the midrib. Intramarginal vein is
close to the edge of the leaf blade. The size of leaf was measured about 11 to 23 cm long and 4 to
7.5 cm wide. The leaves in powdered form exhibits pale green color. It is odorless and taste is
slightly bitter. Thin transverse section of leaf reveals the presence of epidermal layer. Below the
upper epidermial layer, compactly arranged round spongy parenchymatous cells were observed.
Powder microscopy showed the presence of fibers, glandular trichomes, cork cells. The
physicochemical parameters like ash values, loss on drying and fluorescence analysis were also
determined following WHO guidelines and their results were noted. The soxhlet extraction of
powdered leaves was carried out for preparation of various extracts with the solvents in
increasing order of polarity viz pet ether, chloroform, methanol and water.Phytochemical study
shows the presence of steroids, triterpenoids and lipids in petroleum ether extract and alkaloids,
carbohydrates and glycosides in chloroform extract. Methanol extract gave positive tests for
saponins, carbohydrates, glycosides, flavonoids and triterpenoids while aqueous extract shows
the presence of saponins, flavonoids and carbohydrates. Chronic constriction injury (CCI)
resulted in the significant development of mechanical hyperalgesia, heat hyperalgesia and cold
allodynia indicating the induction of painful peripheral neuropathy. CCI induced neuropathy was
associated with significant inflammation as assessed by marked increase in MPO and TNF-
levels and also rise in calcium levels and oxidative stress (decrease in GSH and increase in
TBARS). The activity of chloroform and methanol extracts was evaluated in CCI model of
neuropathic pain. Both the extracts were evaluated at the two dose levels i.e.
100 mg/kg and 200 mg/kg, orally for 14 days. Methanol extract at a dose of 200 mg/kg oral
showed higher protection against decrease in nociceptive threshold and rise in oxidative stress
markers (decreased GSH levels and increased TBARS levels), rise in calcium levels and also rise
August 22, 2014


in inflammatory markers MPO and TNF- levels. So, it is concluded that A. scholaris possesses
significant anti nociceptive activity and protection against oxidative stress and inflammatory
markers.

PCOG-13

STABILITY STUDIES ON CENTELLA ASIATICA EXTRACTS

Ishtdeep Kaur,* Nancy and Gulshan Bansal*
147002, India.

Abstract
Herbal plants have been widely used in the treatment of varied disorders of central nervous
system and autonomic nervous system throughout the world. The lead molecules in these
medicinal plants exert their effects on the nervous system in a mild and safer way, without
causing any physical or psychological dependence, unlike chemically synthesized medicines.
However, the herbal raw material is prone to lot of variation due to several factors such as
identity of plant and seasonal variation at the time of collection, the ecotypic, genotypic and
chemotypic variations, drying and storage conditions and the presence of xenobiotics which
greatly affects the quality, safety and efficacy (QSE) of the herbal preparation. Drugs Regulatory
Agencies like WHO, ICH and EMEA have laid down various guidelines for estabilishing
quality, safety and efficacy of herbal products through stability studies. The present study reveals
the systematic stability testing done on different types of dried extracts (with and without
preservative) of Centella asiatica, a CNS active herb. The dried extracts of Centella asiatica
were studied for comparison of physical, chemical and pharmacological activities under long
term stability conditions (302C/655%RH) and accelerated stability conditions
(402C/755%RH) as per ICH guidelines. A HPLC-UV method for quantitative determinations
of markers compounds as well as for generating chromatographic fingerprint was developed and
validated. Each stability sample was also evaluated for therapeutic activities like free radical
scavenging activity and anticholinestrase activity using in-vitro methods.

PCOG-14

FRUIT PEELS AS POTENTIAL ANTICANCER AGENTS

Kusum Lata*, Vandana Garg, Munish Garg
Email : latakusum22@gmail.com

ABSTRACT
Fruits are widely consumed and considered to have various health benefits and generally their
peels are discarded as waste materials. But some recent studies have proved that fruit peels
August 22, 2014


contain many bioactive components concentrated in them which are very beneficial and have
medicinal properties. The present research compares the anticancer potential of five methanolic
fruit peel extracts (banana, guava, lemon, orange and papaya) on HepG2 cell lines by MTT ([3-
[4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide]) assay. The results showed that
among these fruit extracts, papaya peel extract is most potent anticancer agent with IC
50
value of
18.5g/ml.

PCOG-15

PHARMACOGNOSTIC AND ANALGESIC ACTIVITY SCREENING STUDIES OF
ADVENTITIOUS ROOTS OF FI CUS RELI GI OSA LINN

Mamta Wadhawan, Lavi Rajput* and Balbir Singh
Email- balbir_gndu@yahoo.com

Abstract
Authentication of plant material is prerequisite before using it as research material. Therefore it
was planned to establish pharmacognostic standards. Ficus religiosa was subjected to
macroscopic and microscopic studies. The adventitious roots were dark brown in colour,
aromatic in odour and tasteless. Roots were long and slender. Thin transverse section of
adventitious roots revealed the presence of epiblema, endodermis and epidermis. Differentiation
of protoxylem, metaxylem and pith was clearly noticed. Powder microscopy showed the
presence of thick walled cork cells, cells with brownish content, starch grains, rhomboidal
calcium oxalate crystals and fibres. The soxhlet extraction of powdered adventitious roots was
carried out for preparation of various extracts with the solvents in increasing order of polarity viz.
petroleum ether, chloroform, methanol and water. The phytochemical screening of extracts
revealed the presence of alkaloids, carbohydrates, phytosterols and terpenoids in chloroform
extract. Methanol extract gave positive tests for terpenoids, phenolic compounds, phytosterols,
glycosides, alkaloids, saponins and carbohydrates. Aqueous extract gave positive results for
carbohydrates, proteins, glycosides, saponins, phenolic compounds while flavanoids were found
only in methanol extract. Petroleum ether extract was reported to contain phytosterols and
terpenoids. The analgesic properties were studied using central models i.e. hot plate model and
tail flick model and peripheral model i.e. acetic acid induced writhing model. All the extracts
were evaluated at the two dose levels i.e. 100 mg/kg and 200 mg/kg. The methanol extract at the
dose of 200 mg/kg showed significant analgesic effect in mice in comparison to control and
standard. On the basis of the significant analgesic effect of methanol extract, it was further
subjected to column chromatography using hexane and hexane:chloroform as eluents. At the
10% polarity of the column, the compound MA-6 was isolated. Compound MA-6 had been
characterized with the help of
1
H NMR. The compound obtained was
a pentacyclic triterpenoid (tetrahydro-4,5-dihydroxy-2-(hydroxymethyl)6(icosahydro-
3a,5a,5b,8,8,11a hexamethyl-1-(prop-1-en-2yl) 1H cyclopenta[a]chrysen-9-yloxy)-2H-pyran-3-
yl benzoate) So, it is concluded that adventitious roots of F. religiosa possesses significant
analgesic activity which may be due to the presence of triterpenoids.
August 22, 2014


PCOG-16

EVALUATION OF ANTIANXIETY ACTIVITY OF CALOTROPI S GI GANTEA L.
ROOTS

Nittya K. Dogra
*
, Deepak Kumar, Suresh Kumar
Punjab, India

ABSTRACT
The present study was planned to investigate Calotropis gigantea (L.) Dryand roots (Milkweed;
family Asclepiadaceae) for antianxiety activity studies with a view to validate its traditional
claims. Properly identified powdered plant material was extracted successively using solvents in
increasing order of polarity viz., petroleum ether (60-80C), chloroform, methanol and water. All
the crude extracts were evaluated for antianxiety activity using elevated plus maze model at the
dose levels of 100, 200 or 400 mg/kg, p.o. The bioactive extract was partitioned successively
using solvents in increasing order of polarity viz., n-hexane, ethyl acetate and n-butanol. All
fractions were also subjected to antianxiety activity at the doses of 25 or 50 mg/kg, p.o. Amongst
various extracts, only methanol extract exhibited significant antianxiety activity with respect to
control at the dose of 200 mg/kg. A slight decrease in anxiolytic activity at higher dose (400
mg/kg) of methanol extract was observed. This observation suggests mild sedative activity at
higher dose of methanol extract. Amongst various fractions, only ethyl acetate fraction exhibited
significant antianxiety activity at the dose of 50 mg/kg with respect to control and equivalent to
the standard drug, diazepam (2 mg/kg, i.p.). Preliminary phytochemical screening of methanol
extract and ethyl acetate fraction showed presence of flavonoids as major class of
phytoconstituents. As flavonoids play a pivotal role in treating brain disorders, it is suggested
that these constituents may be responsible for antianxiety activity. It is finally concluded that the
present studies scientifically validated traditional claims of C. gigantea for antianxiety activity.

PCOG-17

ANTIANXIETY ACTIVITY INVESTIGATIONS ON A. PI NDROW ROYLE AERIAL
PARTS

Priyanka Mittal
*
, Deepak Kumar, Suresh Kumar
Punjab, India

ABSTRACT
Abies pindrow Royle (Himalayan Silver Fir; family Pinaceae) has been traditionally used for
the treatment for anxiety but the plant has not been systematically investigated to validate its
traditional claims. Thus, it was planned to investigate antianxiety activity of various extracts and
fractions of A. pindrow aerial parts using elevated plus maze model (EPM). Properly identified
A. pindrow aerial parts were successively and exhaustively extracted using solvents in increasing
August 22, 2014


order of polarity viz., petroleum ether (60-80C), chloroform, methanol and water. Various crude
extracts were subjected to antianxiety activity at the doses of 100, 200 or 400 mg/kg, p.o. in
mice. The efficacy of A. pindrow was statistically compared with the standard anxiolytic drug,
diazepam (2 mg/kg, i.p.). Amongst various extracts, chloroform and methanol extract exhibited
significant antianxiety activity with respect to control and equivalent to the standard drug at the
dose level of 200 and 400 mg/kg, respectively. The methanol extract was partitioned
successively using solvents in increasing order of polarity viz., n-hexane, ethyl acetate and n-
butanol. All fractions were also subjected to antianxiety activity at the doses of 25 or 50 mg/kg,
p.o. in mice. Only ethyl acetate fraction (EAF) exhibited significant antianxiety activity
equivalent to the standard drug at the dose 50 mg/kg. The present studies scientifically validated
traditional claims of A. pindrow for antianxiety activity. Further, authors are involved in isolating
anxiolytic constituent(s) from bioactive extract / fraction of A. pindrow following antianxiety
activity-guided fractionation.

PCOG-18

COMPARATIVE ANTI-ANXIETY EVALUATION OF TWO OCI MUM SPECIES

Ravinder Kaur*
1
, Hemlata Verma
1
, Nidhi Aggarwal
1
, Richa Shri
1

1
Department of Pharmaceutical Sciences and Drug Research
Punjabi University, Patiala-147002
Email- bal.ravinder89@gmail.com

Abstract
Anxiety disorders are among the most common mental disorders above and beyond depressive
disorders with approximately one-eighth of the world population affected at some point in their
life. In a modern society, these disorders have relatively high prevalence affecting between 10
and 30% of the general population with considerable financial resources. Excessive anxiety can
hamper and damage the quality of life. Benzodiazepines, GABA
A
receptor agonist, buspirone, 5-
HT
1
A receptor agonist, are the clinical therapies chiefly prescribed as foremost medication. All
drug classes currently used are associated with side effects that vary in occurrence and severity.
This consideration implicates the search for new anxiolytic compounds that have a fast onset of
action present with less side effects and a wider safety margin. About 43% of anxiety sufferers
use complementary therapy among which herbal treatment is the most popular one. In this
context two species of Ocimum namely O. kilimandscharicum Guerke and O. gratissimum were
evaluated for anxiolytic effect using Elevated Plus maze model. Extracts were prepared for both
the plants using successive solvent extraction. Results showed that aqueous extract of
O.gratissimum was most active at 100mg/kg whereas for O. kilimandscharicum Guerke
methanol extract was most active at same dose. Further studies are under consideration for
identification of phytoconstituents responsible for anti-anxiety effect.

August 22, 2014


PCOG-19

BIOACTIVITY DIRECTED ISOLATION OF ANTIANXIETY CONSTITUENT OF
PI NUS ROXBURGHI I SARG.

Neelam Kumari, Shivani Thakur* and Balbir Singh
email- balbir_gndu@yahoo.com

Abstract
Objectives: The present study was undertaken with an objective to isolate anxiolytic constituent.
Materials and methods: Various crude extracts of the dried bark were prepared using petroleum
ether, chloroform, methanol and water. The methanol extract was evaluated for antianxiety
activity in mice using elevated plus maze (EPM apparatus) at doses of 100, 200 and 400 mg/kg
and further it was loaded onto a column packed with silica, and eluted with chloroform,
chloroform-ethylacetate as the mobile phases.
Results: Phytochemical screening of the methanol extract revealed the presence of alkaloids,
glycosides, saponins, tannins and flavonoids. A total of 210 fractions, each of 250 ml were
collected and distilled to 5-6 ml, were pooled based on similar thin layer chromatograms and
obtained 6 fractions (F
1
-F
6
). The maximum bioactive fraction F
5
(40 mg/kg), when subjected to
qualitative phytochemical screening, tested positive only for flavonoids. TLC of F
5
using mobile
phase toluene:ethylacetate:formic acid::6:2:1 showed two spots. Repeated preparative thin layer
chromatography of F
5
using the solvent system toluene:ethylacetate:formic acid::6:2:1, yielded
two pure isolates NK
1
and NK
2
.These were evaluated for antianxiety activity at doses 10 and 20
mg/kg, NK
1
exhibited antianxiety activity in EPM assay, light/dark chamber and double mirrored
chamber model of anxiety at 20 mg/kg, which was further characterized by spectroscopic
techniques as 2-(3,4-Dihydroxy-phenyl)-5,7-dihydroxy-3-(3,4,5-trihydroxy-6-methyl-tetrahydro-
pyran-2-yloxy)-chromen-4-one, (Quercetrin) and responsible for antianxiety activity of P.
roxburghii.

PCOG-20

EVALUATION OF ANTI-ANXIETY AND ANTI-EPILEPTIC ACTIVITIES OF
GREWI A ASI ATI CA LEAVES

Shabnampreet Kaur, Harpreet Kaur* and Sarabjit Kaur

Abstract
The present study was an endeavor to evaluate anti-anxiety and anti-epileptic activity of ethyl
acetate and methanolic fraction of Grewia asiatica leaves. The shade dried leaves were coarsely
powdered and subjected to methanolic fraction and ethylacetate fraction by using column. Two
doses 100 mg/kg and 200 mg/kg of methanolic and ethylacetate fraction of Grewia asiatica
leaves were used to evaluate anti-epileptic activity. Anti-epileptic activity was evaluated in rats
August 22, 2014


by using maximal electro-shock model and anti-anxiety activity was evaluated using elevated
plus maze model,light and dark model and mirror chambered model. In anxiety model the
standard drug used as diazepam 2 mg/kg. Grewia asiatica leaves fraction significantly decrease
epilepsy and anxiety in all models.

PCOG-21

NATURAL IMMUNE MODULATORS

Shanti devi*, Priya sharma
Babitanalwa47@gmail.com

Abstract
The immune system is said to be a necessary evil as on one hand it protects our body against a
variety of diseases when acting normally, while on the other hand it can create havoc if it gets
distorted from its normal functions. This review is an attempt to highlight the various plants and
their phytoconstituents possessing immunomodulatory activity (in-vitro & in-vivo). Literature
claims that traditional systems of medicine have ample examples of plants possessing
immunomodulatory activity and both immunostimulants and immunosuppressants can be
obtained from natural sources as evident by in-vitro and in-vivo models. Further, various plant
constituents like polysaccharides, tannins, flavonoids etc have been pharmacologically proved to
be potential for immunomodulation. Most five powerful natural immune modulators are :
Magnesium, Vitamin D3, Curcumin, Blackseed oil, Oleander Extract. We conclude that the
various plants and their phytoconstituents can be considered as a valuable source of unique
natural products for development of medicines for immunomodulation. These plants based
products can be incorporated in the main streamline of medicinal treatments. In addition, these
products have always proved to be advantageous over their synthetic counterparts thus the future
of plant derived immunomodulators seems to be very bright.

PCOG-22

EFFECT OF ALCOHOLIC EXTRACT OF ADI ANTUM CAPI LLUS-VENERI S ON
COLD IMMOBILIZATION STRESS INDUCED LIPID PEROXIDATION IN RATS

Nitin Verma
Dean, School of Pharmacy and Emerging Science, Baddi University of Emerging Science and
Technology, Makhunmajara, Baddi Distt. Solan, H. P. 173205, India.
nitin.verma@baddiuniv.ac.in

Abstract:
Global search is on, for the development of an effective antistress drug from natural source
which could effectively tone up the disturbed physiological functioning of the subjects affected
August 22, 2014


by stress problem. Many marketed formulations claim to possess antistress action, but still many
herbs which have claims to be general tonics need to be investigated and their claims be
authenticated. In recent era there is great thrust on screening of herbs for their antistress activity.
Adiantum capillus-veneris belonging to the Adiantaceae family is one of the most common and
widely distributed species and is commonly known as Hansraj. Adiantum species have been used
in traditional Chinese medicine to cure human and animal diseases and a rich source of
triterpenes with various structural skeletons. Flavonoids, phenyl propanoids and sterols have
been isolated from the genus Adiantum. These compounds have been reported to show various
bioactivities, such as analgesic, antinociceptive, anti-implantation, and antimicrobial activities.
Ethanolic extract of the Adiantum capillus-veneris was studied on cold immobilization induced
lipid peroxidation in albino rats. Administration of its extract at a dose of 500 mg/kg b. w.
significantly inhibited cold immobilization stress induced increases in lipid peroxidation in the
liver and brain of albino rat. The results of the present investigations suggest the potential use of
the plant for decreasing anxiety and stress in many emotional and physical disorders.

PCOG-24

ANTIOXIDANT ACTIVITY OF HYDROETHANOLIC EXTRACT OF FRUIT PEEL OF
CI TRUS LI METTA VAR.MI THA

A. Pandurangan, Amandeep Kaur, Diksha Sharma*
M.M. College of Pharmacy, M.M. University, Mullana, Ambala, Haryana, India-133207

Abstract
Citrus limetta is a species of citrus, commonly known as sweet lime, sweet lemon, and sweet
limetta. The present paper aims to evaluate antioxidant activity of hydroethanolic extract of
Citrus limetta fruit peel. The genus citrus (Rutaceae) comprises of trees, shrubs and herbs of
various sizes and uses. They are the most widespread arboreal plants in the world and represent
one of the most important crops. Citrus fruits belong to six genera (Fortunella, Eremocitrus,
Clymendia, Poncirus, Microcitrus and Citrus), which are native to the tropical and subtropical
regions of Asia, but the major commercial fruits belong to genus Citrus. The genus citrus is
represented by 10 species in Pakistan. Citrus limetta var. Mitha (sweet lime) is one of them. It is
popular indigenous citrus fruit relished for its cooling and therapeutic effects and also as a
culinary delight in some parts of the subcontinent. In the traditional indigenous medicinal
system, sweet lime juice is valued for curing fever, malaria and jaundice. The antioxidant
activities of the hydroethanolic extracts have been evaluated by using different in vitro assays
and the results were compared with the standard antioxidants such as butylated hydroxytoluene
(BHT), ascorbic acid, curcumin, quercetin, etc. The overall results of this study indicates that the
extract from stems have potential free radical scavenging activity for treatment of diseases.

August 22, 2014


PCOG-25

HYPOLIPIDEMIC, HYPOGLYCEMIC AND ANTIOXIDANT POTENTIAL OF
J ASMI NUM GRANDI FLORUM ETHANOLIC LEAVES EXTRACT IN
STREPTOZOTOCIN INDUCED- EXPERIMENTAL DIABETES

Akash Jain
1*
, Jasmine
1
, Vipin Saini
1
and Sunil Sharma
2

1 M. M. College of Pharmacy, M.M.University, Mullana-Ambala, Haryana-133207, India
2 Department of Pharmaceutical Science, G. J. University, Hissar, Haryana-125001, India
akash2911@gmail.com

Abstract
Jasminum grandiflorum is scrambling sub erect twining evergreen shrub, native to India, France,
Italy, China, Japan, Morocco and Egypt belonging to family oleaceae, used for ulcerative
stomatitis, leprosy, skin diseases, ottorhoea, otalgia, strangury, dysmenorrhoea, ulcers etc. The
present study was designed to evaluate the hypolipidemic, hypoglycemic and antioxidant
potential of Jasminum grandiflorum leaves extract in streptozotocin induced-diabetic rats.
Experimental hyperglycemia was produced in rats by single dose of Streptozotocin (55 mg/kg
i.p.). Treatment with Jasminum grandiflorum ethanolic extract normalizes the altered lipid
profile and significantly improves the elevated glucose level, glycosylated hemoglobin and
antioxidant parameters (Malondialdehyde and Glutathione level) in dose dependent manner.
Moreover, extract has also shown significant blood glucose lowering effect in oral glucose
tolerance test (OGTT).
Keywords Jasminum grandiflorum, Hyperglycemia, Diabetes, Antioxidant, Hypolipidemia

PCOG-26

SUPERCRITICAL FLUID EXTRACTION

Kusum Kaushik* , Priya Sharma
LR Institute of Pharmacy, Solan
email:kaushikkusum081994@gmail.com

Abstract
Extraction involves the separation of medicinally active components from the crude samples.
The principle methods of extraction are maceration, percolation, digestion, infusion and
decoction. Supercritical Fluid Extraction (SFE) is the process of separating one component (the
extractant) from another (the matrix) using supercritical fluids. Supercritical fluid extraction is
the most effective and efficient way to extract valuable chemical constituents. Supercritical
fluids have unique property which differentiates it from the conventional solvents that it exists in
a fluid phase and processes characteristics between those of gases and liquids at a temperature
and pressure above its critical point. A supercritical fluid can be separated from analyte by
simply releasing pressure, leaving almost no trace and yields a pure residue. Commonly used
supercritical fluids include supercritical carbon dioxide, water and ethanol. It utilizes non-toxic,
August 22, 2014


inexpensive solvents. CO
2
is the king of extraction solvents for botanicals. Extraction conditions
for supercritical CO
2
are above the critical temperature of 31C and critical pressure of 74 bar. It
is a fast process completed in 10 to 60 minutes. Due to its low critical temperature 31C, CO
2
is
known to be perfectly adapted in food, aromas, essential oils and nutraceutical industries. It can
diffuse through solids like a gas, and dissolve materials like a liquid. Supercritical fluids are
suitable as a substitute for organic solvents in a range of industrial and laboratory processes.

PCOG-27

VALUE OF DIGITALIS IN HEART FAILURE

Manmohan Chauhan*, Dr. D.S. Rathore, Priya Sharma
pharmakon.1506@gmail.com

Abstract
Digitalis is a drug containing cardiac glycoside and is obtained from the dried leaves of Digitalis
purpurea family Scrophulriaceae. Digitalis is reported by another name i.e. Foxglove leaves. It is
cultivated in England, Europe , United States and in India. Digitalis purpurea was used in the
18th century and it was considered as a blessing for people suffering from dropsy and in an
attempt to cure illness such as asthma and epilepsy. It requires acidic, sandy soil with traces of
manganese and the temperature needed is 20-30C. Digitalis seeds are sown in nursery beds and
young seedlings are transplanted into the soil. The activity of leaves is due to glycosides.
Digitalis leaves are dark greyish green and slight odour and bitter taste. Digitalis contains
primary glycosides like purpurea A and purpurea B and secondary glycosides. Digitalis
glycosides have five membered ring are C
23
glycosides called as cardenolides and these are
identified with the help of legal test. Digitalis is used in congestive heart failure, asthma and
epilepsy. It shows various side-effects like irregular heart function, stomach upset and small eye
pupil. the allied drugs of Digitalis are D.lanata, D. lutea and D. thapsi and its adulterants are
mullelin leaves[Verbascum thapus] and comfrey leaves [Syphytum officinale]. The poster will
include the toxicity profile, dozing and drug interaction profile.

PCOG-28

MICROWAVE ASSISTED EXTRACTION A NOVEL PROMISING TECHNIQUE
FOR EXTRACTION OF MEDICINAL PLANTS

Shivani Dhiman, Sunil Kumar, Vineet Mittal
Department of Pharmaceutical Sciences, MDU, Rohtak-124001

Abstract
Microwave energy is a non-ionizing electromagnetic radiation having frequency 0.3-300 GHz.
Microwaves penetrate biomaterials and interact with polar molecules such as water in the
August 22, 2014


biomaterials to create heat. The microwave heating leads to the expansion and ruptures of cell
walls and is followed by the liberation of chemicals into the solvent. As MAE depends on the
dielectric susceptibility of solvent and matrix, polar solvents like water and alcohol may give
efficient extraction. In common practice binary mixture of hexane and acetone is used, in which
one solvent will absorb the microwave energy. MAE has a number of advantages like shorter
extraction time, less solvent consumption, higher extraction rate and lower cost as compare to
conventional extraction methods. The use of MAE in natural products extraction started in the
late 1980s, and through the technological developments, it has now become one of the popular
and cost-effective extraction method for the herbs.

PCOG-29

A SYSTEMATIC REVIEW OF THE DRUG :-PANAX GI NSENG

Navneet Sharma* , Priya Sharma
LR Institute of Pharmacy, Solan
Email : sharmanavneet435@gmail.com

Abstract
P.ginseng, P.japonica, P.notoginseng, P.quinquefolium belonging to family Araliaceae. It is also
known by the other names like ninjin, pannag, panax. It grow widely in Korea, China and Russia
and has been used as a herbal remedy in eastern asia for thousands of the years. The propagation
of ginseng is done by means of seeds. The seeds are sown in nursery beds. There are three types
of nursery beds i.e. Yang-jik, To-jik and Ban-yang-jik. Ginseng roots are tuberous corpulent and
are yellowish-brown, white or red. Ginseng is the mixture of the several saponin glycosides. The
main active component of Panax ginseng are ginsenosides, panaxosides. Ginseng is used to
increase stamina, for better performance, better focus, as a stimulant and sedative. Ginseng has
been used for the treatment of various cancers like breast, liver, lung and skin cancer. Various
side effects of the ginseng are to be reported like insomnia , increase heart rate, increase or
decreased blood pressure, headache, loss of appetite, diarrhoea, itching, rashes, mood change.
According to the American dosing for reducing the blood sugar after a meal in people with type
2 diabetes: 3 gram up to 2 hrs before a meal orally : for treating type 2 diabetes : 200 mg daily.
For erectile dysfunction : Panax ginseng upto 900 mg three times a day. The poster will also
include the marketed formulation, dosing and toxic effects of the drug.

August 22, 2014


PCOG-30

INCORPORATING HERBAL MEDICINE INTO CLINICAL PRACTICE

Supriya Verma*, Dr. DS Rathore, Priya Sharma
E Mail: kk081994@gmail.com
Abstract
Herbal medicine has been used worldwide throughout history. The World Health Organization
estimated that perhaps 80% of the worlds people relied on herbs for their primary healthcare
needs. Due to such an extensive use of herbal drugs throughout the world there is a need to
incorporate herbal medicine into clinical practice. There are various systems of medicine which
rely on herbs as their basic chemical entity for example ayurveda, Chinese system of medicine,
unani and siddha system of medicine. Some countries consider drugs from herbal origin as drugs
while other consider them as dietry supplements and therefore they are regulated accordingly. In
India herbal medicines are regulated just like other conventional drugs and the department of
AYUSH (Ayurveda Yoga and Naturopathy, Unani, Siddha and Homeopathy) gives these
regulations. The toxicity and efficacy of herbal medicines needs to be studied intensively and
more research has to be done in this field. Further to be discussed are the various interactions of
herbs their safety and efficacy profile. Herbalism is the use of plants for medicinal purposes and
the study of such use. To maximize the use of natural drugs their must be an International co
ordination and hamonization on the regulatory requirnments.when this harmonization would
actually be acchieved botanicals would be explored more and it will boost there usage globally.

PCOG-31

MICROWAVE ASSISTED EXTRACTION

Shalini Kanwar* Dr. DS Rathore, Priya Sharma
Email: pharmakon.1506@gmail.com

Abstract
Extraction is the process of removal of active chemical constituents from solid or liquid mixture
with a liquid solvent. Microwave is defined as the portion of the electromagnetic speed spectrum
that is situated between radio waves and infrared radiations. Its frequency ranges from 300MHz
to 300GHz. They are the principle carriers of television and telephone signals. Microwaves are
absorbed by water in foodstuffs. Materials such as glass and ceramics do not absorb microwaves
and instead, they metals reflect them. Microwave Assisted Extraction (MAE) is the process of
heating solvents in contact with a sample with microwave energy to partition compounds of
analytical interest from sample matrix into the solvent. It has certain advantages over other
conventional techniques such as it has shorter extraction time, allows to reduce solvent
consumption with shorter cooling time. MAE has been shown to be cost effective when
compared to other extraction methods. MAE has a wide range of applications in the extraction of
August 22, 2014


phytoconstituents such as the extraction of polyphenols from Green tea, Embelin from Embelia
ribes, Cocain from coca leaves and glycerrhizic acid from Liquorice. Hence the development of
new extraction techniques will give rise to the discovery of new effective compounds from
phytopharmaceutical source. MAE has risen rapidly in the last decade and for most applications
it has proven to be much more effective in all aspects as compared to the traditional extraction
techniques.

PCOG-32

VOLATILE OILS; A PRESICIOUS GIFT OF NATURE

Sunil Saharan, Sandeep Kumar, Pradeep Kamboj
JCDM College of Pharmacy,
Barnala Road, Sirsa, Haryana 125055
Email: saharansunil5444@gmail.com

Abstract
Volatile oils or Etheral oils are extremely complex and possibly the most interesting herbal
elements that provide the herbalists with a potent aid for their treatments. These oils are actually
the combinations of oxygenated compounds and hydrocarbons derived from the herbs. The
volatile oil is composed of various active phytoconstituents and extensively used in the
formulation of many types of medicines in the modern system of medicine. It is used in the
flavouring industries, as perfuming agents in the pharmaceutical preparations, foods & beverages
and also in cosmetics industries. Waste of this oil is also used as additives in the powder
formulations, paper industries, formulating churana, bhasma and leh preparations. The volatile
oils are one of the major class of secondary metabolites and used extensively as an important
ingredient in preparation of wide range of medicines. Hence, the present review is undertaken to
explore and report the physical and chemical nature, distillation methods and future prospective
of volatile oils.

PCOG-33
CRATAEVA NURVALA: A VALUABLE MEDICINAL PLANT

Vandana Valecha
Doon Valley Institute of Pharmacy and Medicine, Karnal

ABSTRACT
The discovery of a novel chemical component from a medicinal plant may form the basis of
development of various therapeutic agents with better activity. More than 500 medicinal plants
have been reported to possess medicinal properties. Crataeva nurvala Buch Ham, (family:
Capparidaceae) is one of the most common species among them. The whole plant posses high
medicinal value and traditionally used in treating various ailments for human beings. The plant is
used internally as well as externally. Externally, the paste or its leaves or skin of bark is applied
August 22, 2014


in cervical adenitis, abscess and edematous wounds. The same paste is salutary in rheumatic joint
for relief of pain. The pulp of leaves is applied on abdomen in splenic enlargement, with great
benefit. Internally, varuna is used in vast range of diseases. The decoction of leaves given along
with ghee relieves flatulence and abdominal pain. It also works well as a laxative, cholegogue,
appetizer and vermicide, hence useful in anorexia, tumors, liver disorders, flatulent dyspepsia
and helminthiasis. Phytochemically the plant has been investigated for flavonoids,
glucosinolates, plant sterols, including lupeol, saponins, tannins, cardenolides, alkaloids,
triterpenes and saponins. The plant has been demonstrated to possess multiple pharmacological
activities such as antiinflammatory, urolithiatic, antidiabetic, antibacterial, analgesic,
antiinfertility, antidiarrhoeal, antinociceptive and cardioprotective activity.

PCOG-34

EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF
GLYCYRRHI ZA GLABRA LINN. ROOTS EXTRACTS

Chetan Sharma* and K.R. Aneja
*Department of Microbiology, Guru Nanak Khalsa College, Yamuna Nagar- 135 001
Department of Microbiology, Kurukshetra University, Kurukshetra136119
Email: Chetanmicro147@gmail.com

ABSTRACT
Glycyrrhiza glabra, commonly called as Licorice, is one of the important traditonal medicinal
plants grow in the various part of the world and roots of this plant has been used for several
medicinal purposes. Antimicrobial activity of G. glabra root extracts was studied by agar well
diffusion method against the six ear pathogens namely, Staphylococcus aureus, Proteus
mirabilis, Escherchia coli, Pseudomonas aeruginosa, Acinetobacter sp. and Candida albicans.
The antibacterial and antiyeast activity of G. glabra root extracts on the agar plates varied in
different organic (methanol, ethanol and acetone) and aqueous (hot and cold) extracts. All the
fruit extracts of T. belerica showed antibacterial activity against all the tested bacterial isolates
with zone of inhibition ranging between 26.3mm and 14.6mm, maximum against S.aureus
(26.3mm in methanolic extract) whereas in case of yeast, it ranges from 23.6mm and
15.3mm.However aqueous extract displayed activity against three tested pathogens with zone of
inhibition ranging between 20.3mm and 14.6mm.The MIC value for G. glabra root extracts
ranged between 3.12mg/ml and 25mg/ml and MBC value ranged between 6.25mg/ml and
25mg/ml. The lowest MIC of 3.12mg/ml and MBC of 6.25mg/ml was observed against S.aureus.
Methanolic extract of G. glabra was found best among all the tested solvents. The methanolic G.
glabra extract was screened for free radical scavenging effects at various concentrations (10, 30
and 50g/ml) by DPPH free radical scavenging method, Reducing power activity and Nitric
oxide radical scavenging activity. All these antioxidant activities were concentration dependent
which were compared with standard antioxidants such as BHT and ascorbic acid.

August 22, 2014


PCOG-35

ANTIDIABETIC HERBAL FORMULATIONS AVAILABLE IN MARKET

Kunwar,
*
Maninder Kaur
Assistant Professor, Department of Pharmacology, Doon Valley Institute of Pharmacy &
Medicine, Karnal.
E-Mail Maninder3Maninder@gmail.com

Abstract
The present study was based on Diabetes, its cure & herbal products available in market.
Diabetes mellitus is the most common endocrine disorder, affecting 16 million individuals in the
United States and 200 million worldwide. Despite the use of advanced synthetic drugs for the
treatment, use of herbal remedies is gaining higher importance because of synthetic drugs have
drawbacks and limitations. The herbal drugs with antidiabetic activity are extensively formulated
commercially because of easy availability, affordability and less side effects as compared to the
synthetic antidiabetic drugs. Antidiabetic herbal formulations (AHF) are considered to be more
effective for the management of diabetes. There are around 600 herbal drug manufacturers in
India of which almost all manufacturers are developing AHF in addition to others. Till date no
article is published to give detailed information of the herbal preparations on diabetes available
in market. Thus, this review article undertake the attempt for providing updated information on
the type of diabetes and herbal formulations which will enhance the existing knowledge of the
researchers.

PCOG-36

PHARMACOGNOSTICAL AND PHYSICO-CHEMICAL EVALUATION OF THE
LEAVES OF CRYPTOLEPI S DUBI A (BURM.F.) M.R. ALMEDIA

Rajinder Mann
*
, Pradeep Kumar, Asha Rani, Ram Kumar Roy
Doon Valley Institute of Pharmacy and Medicine, Karnal
*
E-mail: rajindermannhcp@gmail.com

Abstract
Cryptolepis dubia (Burm.f.) M.R. Almedia (Asclepiadaceae), commonly known as jambupatra
sariva in Sanskrit and as Dudhi or Karanta in Hindi, it is a large evergreen laticiferous, woody
climbing, perennial shrub common especially in deciduous forest of sub-himalayan tracts, Bihar,
Orisa, East Uttar Pradesh in Varanasi region. Cryptolepis dubia is a very useful plant because of
its multiple uses as a traditional medicine, such as anti-diarrhoeal, anti-bacterial, antiulcerative,
anti-inflammatory, blood purifier and for lactation in women. The leaves were collected during
month of January 2011 from Karnal (Haryana). The dried leaves were powdered, passed through
a 40 mesh sieve and stored in closed vessel for further use. The powder 180 gm was extracted
successively with petroleum ether (60-80
0
C), chloroform, methanol and distilled water in
Soxhlet extractor for 18 hrs. The macroscopic & microscopic characters of leaves were observed.
August 22, 2014


The extractive values of an alcohol and water were observed. The preliminary phytochemical
screening of various extract revealed the presence of alkaloids, glycosides, flavonoids, terpenoids
and steroids present in the different prepared extract. The present study on Cryptolepis dubia will
be useful to supplement information with regards to its identification and authentication.

PCOG-37

CHEMOMETRIC ANALYSIS: A NOVEL TOOL FOR HERBAL DRUGS ANALYSIS

Prerna Sarup
M.M. College of Pharmacy, Maharishi Markandeshwar University, Mullana, Ambala
E-mail- psarup_pu@hotmail.com

Abstract
High performance chromatographic techniques are the most commonly used methods in the
standardization of herbal drugs. But the intricacy involved in the herbal matrices is difficult to
analyze because of their complexity of chemical composition. Chemometric analysis provides a
good opportunity for extracting out more useful chemical information from the original statistics.
Comprehensive methods and hyphenated techniques associated with chemometrics used for
extracting useful information and supplying various methods of data processing are now more
and more widely used in medicinal plants, among which similarity analysis, hierarchical
clustering analysis (HCA) and principal component analysis (PCA) are most commonly used
techniques. This study focuses on the various chromatographic techniques, chemometric tools
and interpretation of results by HCA & PCA in various guggulu samples. The results of HCA
and PCA producing same inference as that of analytical techniques validated and provided proof
to the results of analysis of these samples in their similarity and dissimilarity assessment. In
order to evaluate the discrimination ability of the different constituents, PCA was employed
using the peak areas of all peaks as input data.

PCOG-38

HERBAL MEDICINES FOR THE MANAGEMENT OF DIABETES

Shashikant Bhardwaj
*
Department of Pharmacology, JCDM College of Pharmacy, Sirsa, Haryana
E-mail: shashipharma10@gmail.com

Abstract
Diabetes mellitus a serious chronic disease, and is caused by the abnormality of carbohydrate
metabolism which is linked to low blood insulin level or insensitivity of target organs to insulin.
Diabetes mellitus is a group of metabolic diseases characterized by high blood sugar (glucose)
levels. Diabetes mellitus is known to be associated with obesity, hypertension, hyperlipidemia,
August 22, 2014


neuropathy and cardiovascular diseases. It was reported that in 2010 diabetes affected as many as
285 million people worldwide and resulted in heavy personal and national economic burdens.
Currently available treatment options for this disease are limited and have several adverse
effects. Herbal medicines have been highly esteemed source of medicine throughout the human
history. They are widely used today indicating that herbs are a growing part of modern high-tech
medicine. Plants provide the best option for search of desired safe and effective medications due
to presence of wide variety of compounds. Therefore, systematic and intensive search for new
drugs to treat diabetes mellitus from plants seems to be of great utility to develop novel drug
prototypes. These include Allium sativum, Eugenia jambolana, Momordicacharantia, Ocimum
sanctum, Phyllanthusamarus, Pterocarpusmarsupium, Tinosporacordifolia,
Trigonellafoenumgraecum and Withaniasomnifera. To date, more than 1200 flowering plants
have been claimed to have anti-diabetic properties. Among them, one-third have been
scientifically studied and documented in around 460 publications. The hypoglycemic effect of
some herbal extracts has been confirmed in human and animal models of diabetes.

PCOG-39

HERBAL COSMETICS: AN OVERVIEW

Deepak Dhankhar
*1
, Upma
1
, Priti Mehndiratta
1
, Gaurav Khurana
1
, Jyoti Dahiya,
Minakshi Gupta
1

1
2

Abstract
The pouplarity of herbal cosmetics in society and technological advances in the manufacturing
process has resulted in the flooding of the market with herbal formulations. Recently herbal
cosmetics have gained much recognition and became popular among people. These products
claimed to have efficacy and intrinsic acceptability due to routine use in daily life and devoid of
side effects commonly seen with synthetic product. The herbs used in preparation of these skin
cosmetics are multifuctional with antioxidant, antiinflammatory,antiseptic and antimicrobial
properties. Chemically synthesized, highly concentrated drugs may produce many side
effects.Some of extracts that are used in herbal cosmetics are Amla, Brahmi, Neem, Aloe vera,
Tulsi, Reetha powder. Mainly formulations which are used as herbal Cosmetics are eyeliners,
lipsticks, foundations, perfume. Various applications of Herbal Prouducts in Cosmetics are
Herbal skin care products ( lotions, creams and body powder), herbal hair care cosmetics ( heena,
amla, brahmi, bhringraj, guar gum), Herbal Lip care and eye care cosmetics.

August 22, 2014


PCOG-40

WI THANI A SOMNI FERA AND EVALUATE THE EFFECT OF EXCIPIENTS ON ITS
DISSOLUTION PROFILE

Praveen Kumar Goyal
1
, G.K. Singh
2
, B.P. Nagori
2

1
Alwar Pharmacy College, Alwar-301030 (Raj.)
2
Lachoo Memorial College of Science & Technology (Pharmacy Wing)
Jodhpur-342003, Rajasthan, India
Email: goyalprvn09@gmail.com

Abstract:
Medicinal plants have curative properties due to the presence of various complex chemical
substances of different composition, which are found as secondary plant metabolites in one or
more parts of these plants. The present paper deals with formulation and evaluation of anti-stress
activity of tablets prepared from alcoholic root extract of Withania somnifera and evaluate the
effect of excipients on its dissolution profile. Since withanolides belong to alkaloidal category
and they have less solubility in water, there is need to select appropriate excipients in
formulation to enhance its dissolution in biological fluid. A solid pharmaceutical dosage
formulation using a novel dry plant extract (tuberous roots) using various Excipients viz.,starch,
magnesium stearate, MCC, dibasic calcium phosphate, talc and PEG-4000 by direct compression
was reported to be statically significant as anti-stress formulation. From these four batches (P-1
to P-4); the batch P-2 was found to be the best formulation in terms of better dissolution profile.
And therefore, this batch P-2 was selected for anti-stress activity screening. Finally the anti-stress
activity was reported to be significant. This communication also deals with the evaluation of
formulated tablets (weight variation, friability, hardness and disintegration time).
Keywords:Withania somnifera, tablets, PEG, MCC, Dicalcium Phosphate, Herbal formulation.

PCOG-41

EVALUATION OF ANTIMICROBIAL ACTIVITY OF CORI ANDRUM SATI VUM
FRUIT EXTRACTS AGAINST MICROBES ASSOCIATED WITH FRUIT JUICES

Romika
1
, Neeraj Kumar Aggarwal
2*
, K.R. Aneja
3

1
Research scholar, Department of Microbiology, Kurukshetra university Kurukshetra, India
2
Assistant professor, Department of Microbiology, Kurukshetra university Kurukshetra, India
3
Vaidyanath Research, Training and Diagnostic Centre, Kurukshetra, India
Email neerajkuk26@gmail.com

Abstract
Spices have been used for centuries in Asian countries such as China and India as a food
ingredient. Instead of their flavouring effect, spices also possess antimicrobial effects on plant
and human pathogens. Food processing technologies such as chemical preservatives cannot
August 22, 2014


eliminate food pathogens or delay microbial spoilage totally. The present study was designed to
evaluate the antimicrobial activity of Coriandrum sativum against microbes associated with
juices. Organic (Methanol, ethanol, acetone) and aqueous (hot and cold) extracts from the fruits
of C. sativum were tested for their antimicrobial activity through agar well diffusion method and
minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC) values
were determined through the macrodilution broth method against three different microorganism,
two bacteria (one gram positive and one gram negative) and one yeast. Among the organic
extracts, acetonic extract exhibit antimicrobial activity against all three tested microbes with a
maximum zone of inhibition of 35.48mm against Bacillus cereus, followed by Serratia
marcescens (28.75mm). Alcoholic fruit extracts was found to be active against bacteria and cold
aqueous extract displayed activity only against B. cereus. Hot aqueous extract did not show
antimicrobial activity. Acetonic fruit extract was found to be best against all tested microbes
with lowest MIC of 1.56 mg/ml and MBC of 3.12 mg/ml and showed better antimicrobial
activity than sodium benzoate. Therefore, acetonic extract of C. sativum has a biopreservative
potential in fruit juices.
Key Words:-Coriandrum sativum, biopreservative, MIC, MBC

PCOG-42

Leaf-spot disease of Trianthema portulacastrum a new record from world

Vikas Kumar
1
, Neeraj Aggarwal
2*
1
Research Scholar, Department of Microbiology, Kurukshetra University, Kurukshetra
2
Assistant Professor, Department of Microbiology, Kurukshetra University, Kurukshetra
*email: neerajkuk26@rediffmail.com

Abstract:
Trianthema portulacastrum L., an indigenous plant to South Africa, is a very common weed of
tropical and subtropical areas throughout the world. In the years 2011-13 a series of surveys for
natural enemies of horse pursane were conducted in Haryana, Punjab and Uttar Pradesh. A leaf
spot disease was found on horse purslane at Kurukshetra. A species of Fusarium was isolated on
PDA and TEDA media from infected leaves. On the basis of cultural, morphological and
molecular characteristics, it was identified as Fusarium chlamydosporum Wollenw. & Reinking.
In vitro inoculation on Trianthema leaves, the pathogen showed similar symptoms as occurred in
nature. Thus, proving pathogenicity and Kochs posulates. This is the first report of occurrence
of F. chlamydosporum causing leaf spot on horse purslane from the world.

August 22, 2014


PCOG-43

Alternariamacrospora: A potential fungal pathogen for biocontrol of partheniumweed

M. Kaur
1
, N. K. Aggarwal
2
*
1
Research Scholar, Department of Microbiology, Kurukshetra University, Kurukshetra
2
Assistant Professor, Department of Microbiology, Kurukshetra University, Kurukshetra
*E-mail:neerajkuk26@gmail.com

Abstract
Congress grass, Parthenium hysterophorus L. (Family: Asteraceae; Tribe: Heliantheae), is
known for its notorious role as environmental, medical and agricultural hazards around the
globe. In the years 2012-14, a series of surveys for natural enemies of parthenium were
conducted in Haryana. During this survey, a leaf spot disease has been regularly reported on
congress grass at different parts of the Kurukshetra and its adjoining areas.The pathogenwas
isolated on PDA and PeDA media from infected leaves and preliminary identification showed
that the pathogen belongs to the genus Alternaria.The culture has been deposited to the CABI,
International Mycological Institute (IMI) UK for species identification. The molecular
identification showed that the pathogen is Alternariamacrospora(IMI no. 503549).Literature
study showed that this pathogenhas been reported first time onPartheniumhysterophorusfrom the
world.In vitroand in vivo inoculation on partheniumleaves, the pathogen showed similar
symptoms as occurred in nature, proving pathogenicity and Kochs postulates.
Thephytotoxicityoffungal cultural filtratehas been confirmed on parthenium leaves in lab
conditions. Due to the highly virulent nature of this pathogen, it has been selected for the further
study to develop mycoherbicide to control this devastating weed.

PCOG-44

HERBS & DRUG INTERACTION

Priya Yadav* A.C.Rana, Dhirender Kaushik, Manjusha Chaudhary
Institute Of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra
(priyadav2812@gmail.com)

ABSTRACT
The use of medicinal herbal products as primary, complementary or alternative therapy is
popular globally due to cultural, safety, cost and factors. Advances in western medicine have
dramatically increased health conditions and life expectancy of people. In spite of the latest
developments in allopathic system of medicine people still seek alternative and complimentary
system. The use of herbal medicine to treat a wide range of conditions is rising rapidly, leading
to increased intake of phytochemicals. Recent studies reveal potential fatal interactions between
herbal medicines and drugs. Herbal medicine is amongst the 16 alternative systems of medicine,
which is used to treat existing illness and also for preventive and health conditions. Herbs like
Aloe vera, St Johns wort, Ginkgo biloba, Feverfew, Ginger and Kava make most of the market.
August 22, 2014


The increasing popularity of alternative therapies, including herbal remedies is a new challenge
for health care providers because the evidence on safety for herbal remedies is incomplete,
complex and confusing. The herbal medicines are certainly associated with risks and benefits.
Interaction between herbal medicines and drugs are based on the same pharmacokinetic and
pharmacodynamic principles as like drug-drug interactions. Nowadays patients taking both
ayurvedic and allopathic treatment remain unaware of potential herb-drug interaction.
Pharmacist can change the present scenario and utilize their knowledge in providing healthy
information about herb-drug interaction.

PCOG-45

APPLICATION OF NOVEL ULTRASOUND ASSISTED EXTRACTION TECHNIQUE
FOR THE HERBS

Sunil Kumar*, Vineet Mittal and Shivani Dhiman
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124 001

Abstract
For ultrasound assisted extraction (USAE) sound waves of frequency >20 kHz are used. The
ultrasonic waves are produced by an apparatus equipped with ultrasonic probe, transducer and
digital temperature and frequency control unit. The ultrasound waves generate the expansion and
compression cycle within the solvent which help in diffusion of solvent into the crude drug.
Moreover due to energy produced by sound waves the cells of the drug are also broken as
evident from scanning electron microscopy of sample. The disruption of cell membrane also
helps in washing out of solubilised cell content. For successful application of USAE several
process variables has to be kept in mind like ultrasonic frequency, extraction temperature and
sample solvent ratio. These variables can be changed sensitively to achieve more yield in less
time. Keeping in view the advantage of this novel technique, it can be applied for the extraction
of herbs.

August 22, 2014


PCOG-46

WI THANI ASOMNI FERA AND EVALUATE THE EFFECT OF EXCIPIENTS ON ITS
DISSOLUTION PROFILE

Praveen Kumar Goyal
1
, G.K. Singh
2
, B.P. Nagori
2
Department of Pharmacognosy
1
Alwar Pharmacy College, Alwar-301030 (Raj.)
2
Lachoo Memorial College of Science & Technology (Pharmacy Wing)
Jodhpur-342003, Rajasthan, India
Email: goyalprvn09@gmail.com

Abstract:
Medicinal plants have curative properties due to the presence of various complex chemical
substances of different composition, which are found as secondary plant metabolites in one or
more parts of these plants. The present paper deals with formulation and evaluation of anti-stress
activity of tablets prepared from alcoholic root extract of Withania somnifera and evaluate the
effect of excipients on its dissolution profile. Since withanolides belong to alkaloidal category
and they have less solubility in water, there is need to select appropriate excipients in
formulation to enhance its dissolution in biological fluid. Asolid pharmaceutical dosage
formulation using a novel dry plant extract (tuberous roots) using various Excipientsviz.,
starch,magnesium stearate, MCC, dibasic calcium phosphate, talc and PEG-4000 by direct
compression was reported to be statically significant as anti-stressformulation.From these
fourbatches (P-1 to P-4);thebatch P-2 (Starch 30mg and MCC 35mg) was found to be the best
formulation in terms of better dissolution profile. And therefore, this batch P-2 was selectedfor
anti-stressactivity screening. Finallytheanti-stressactivitywasreportedto be significant. This
communication also deals with the evaluation of formulated tablets (weight variation, friability,
hardness and disintegration time).
Keywords:Withaniasomnifera, tablets, PEG, MCC, Dicalcium Phosphate, Herbalformulation.

PCOG-47

MICROSCOPIC STUDY AND PRELIMINARY PHYTOCHEMICAL INVESTIGATION
OF CHENOPODI UM ALBUM

Pushpander Kumar
*
and Sunil Kumar
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra -136119, Haryana,

Abstract
Chenopodium album (Chenopodiaceae) is commonly known as Bathu. It is used as anti-ulcer,
anti-nociceptive and hepatoprotective, antioxidant anti-helminthic, antipruritic etc. The present
work is done to study microscopy and preliminary phytochemical investigation of Chenopodium
album. Fresh leaf and dried power of the plant were studies macroscopically and
August 22, 2014


microscopically. Preliminary phytochemical investigation of plant material was done. The study
revealed that T.S of leaves showed the presences of xylem covered with phloem.
Parenchymatous cells were observed in epidermis. Preliminary phytochemical screening showed
the presence of alkaloids, flavonoids, phenolic compounds, saponins and glycosides. The
microscopy and physiochemical studies of the Chenopodium album assist in its standardization
for quality, purity and identification.
Keywords: Chenopodium album, fibre, microscopy, Phytochemical, xylem

PCOG-48

MOUTH ULCER PROTECTION BY J ASMI NUM GRANDI FLORUM

1
Shashikant Agrawal,
2
Shakti Goel,
3
Neeraj Gilhotra
1
School of Pharmacy, Suresh Gyan Vihar University, Jaipur
2, 3
Pharmacology Laboratory, Department of Pharmaceutical Sciences, Maharshi Dayanand
University, Rohtak 124 001, Haryana, India

Abstract
The present study aimed to evaluate ulcer protective activity of ethanolic leaf extract of
Jasminum grandiflorum in rat cheek pouch. Cheek pouch ulcers were induced by standard
method of local injection of 50 microlitre of acetic acid. The extract (125, 250 and 500 mg/kg)
was administered topically once a day for 10 days after development of ulcers. Ulcers were
visually recorded by an optical camera and an Ulcer Index was determined. Simultaneously,
urine samples of rats were collected on day 4, 8, 12 in control and treated rats separately.
Positive Control rats were observed to contain higher levels of nitrite (expressed as 24h output)
in their urine. Administration of Ethanolic extract (250 mg/kg) of Jasminum grandiflorum
showed a statistically significant decrease in ulcer index, which was accompanied by a decrease
in urinary nitrite levels. The results served to indicate the possibility of ulcer protective- like role
of Jasminum grandiflorum and a possible nitriergic mechanism as one of the biochemical
mediators of the noted activity.

PCOG-49

A REVIEW- TEAK

Geeta Deswal
Guru Gobind Singh College of Pharmacy, Yamunanagar
Email: - deswalgeeta@gmail.com

Abstract
Traditional system of medicinal consists of large number of plants with various medicinal and
pharmacological importance and hence represents a priceless tank of new bioactive molecules.
August 22, 2014


Tectona grandis (Linn.) tropical hardwood tree is one amongst these, found to south and
southeast Asia, mainly India, Indonesia, Malaysia, and Myanmar, but is naturalized and
cultivated in many countries, including those in Africa and the Caribbean. Myanmar accounts for
nearly one third of the world's total teak production. It is commonly known as Sagun or
Sagwan, and has been recognized in different traditional system of medicines for the treatment
of various diseases of human beings. Different parts of this plant are traditionally claimed to be
used as anti-fungal, anti-anemic, antioxidant, anti-fertility, anti-ulcerative, nitric oxide
scavenging activity, anti-viral activity, wound healing activity, laxative, scabies, bronchitis and
hair promoter to list of few. Therefore, the present review aimed to compile up to date and
comprehensive information of teak with special emphasis on its photochemistry, various
scientifically documented pharmacological activities, traditional and folk medicine uses.

August 22, 2014


MISCELLANEOUS
ABSTRACTS

August 22, 2014


MIS-1

STANDARDIZATION OF MISWAK: AN ORAL HYGIENE TOOL

*a
Manu Arora,
b
Anees A Siddiqui,
c
SarveshPaliwal,
a
AnkitaKapoor,
a
Ramit Kapoor
*
a
School of Pharmacy & Emerging Sciences, Baddi University of Emerging Science &
Technology, Makhnumajra, Baddi, Distt.Solan, Himachal Pradesh-173205, India.
b
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, JamiaHamdard, New Delhi,
110062, India.
c
Department of Pharmacy, Banasthali University, P.O. Banasthali Vidyapith,Distt. Tonk,
Rajasthan, 304022, India.

Abstract:
Miswak is a natural oral hygiene tool belongs to the family Salvadoraceae. It has been proven
multipurpose tree. It is also known as toothbrush tree because of its oral hygiene properties. It
contains number of chemical constituents of different categories. Miswak is well known for its
therapeutic activities like anti-microbial, antibacterial, carminative, anti-fungal etc. The
objective of the research was to study pharmacognostical and physicochemical parameters to
confirm the identity of plant. Extraction was done by maceration process by using chloroform,
methanol and water as solvents. Its chemical evaluation was done by using different chemical
tests. Other parameters like ash value, extractive value, moisture content and foreign matter were
also studied and recorded. The extracts Salvadorapersicaaerial parts showed the presence of
phytoconstituents such as alkaloids, glycosides, terpenoids and flavonoids. From the above
studies it can be concluded that the various parameters such as pharmacognostical and
phytochemical parameters of the aerial parts of Salvadora persica may be utilized for its
identification and differentiation from other species. Alkaloids, terpenoids, glycosides, steroids
and polyphenolic like secondary plant metabolites which are present in this plant may be
responsible for its pharmacological activities. The road ahead is to establish specific bioactive
molecules, which might be responsible for these actions. Therefore the cultivation, collection,
and further exploration of S.persicaare essential.

MIS-3

ANTIMICROBIAL RESISTANCE - A GLOBAL CONCERN
NO ACTION TODAY, NO CURE TOMORROW.

UmanshuDhingra*, VandanaSaini and AnjuGoyal
Chitkara College of Pharmacy, Chitkara University, Rajpura-140 401 (Punjab)
anju_goyal2003@rediffmail.com

Abstract
The World Health Organization has selected combat antimicrobial resistance as the theme for
World Health Day 2011. Antimicrobial resistance i.e. (AMR) is resistance of a microorganism
August 22, 2014


(bacteria, viruses and some parasites) to an antimicrobial medicine to which it was previously
sensitive, has developed into a major public health crisis from the overuse and misuse of
antibiotic drugs. Inappropriate and irrational use of medicines provides favourable conditions for
resistant microorganisms to emerge and spread. A high percentage of hospital-acquired
infections are caused by highly resistant bacteria such as Methicillin-Resistant Staphylococcus
aureus (MRSA) and vancomycin-resistant enterococci. Ciprofloxacin is the only antibiotic
currently recommended by WHO for the management of diarrhoea due to Shigella organisms. As
per Centre for Diseases Control and Preventions (CDC) trend in AMR, reports of methicillin-
resistant Staphylococcus aureus (MRSA), a potentially dangerous type of staph bacteria that is
resistant to certain antibiotics and may cause skin and other infections. AMR has also been noted
with some of the drugs used to treat human immunodeficiency virus (HIV) infections and
influenza. National Institute of Health (NIH) has given various contributions in the previous
year towards AMR, some of them include NIH funded 4 clinical trial to fight against AMR
NIAID ON Oct 19 2010. Role and efforts are made by WHO towards the cause to draft Global
action plan on antimicrobial resistance. WHO is engaged in guiding the response to AMR
through: policy guidance, support for surveillance, technical assistance, knowledge generation
and partnerships, including through disease prevention and control programmes; essential
medicines quality, supply and rational use.

MIS-4

PARAGLIDING THROUGH SWOT ANALYSIS ON USA'S CURRENT REGULATORY
ENVIRONMENT

Manisha
1
, Harish Dureja
1
, Swagat Tripathy
2
1
Department of Pharmaceutical Sciences, MaharshiDayanand University, Rohtak
2
Apotex Res. Pvt. Ltd., Bangalore
Manishasaini1461@gmail.com

Abstract
To sustain in competitive environment, a great deal of self-reflection, analysis and strategic
planning is required. SWOT analysis is a tool that identifies the strengths, weaknesses,
opportunities and threats. USA pharmaceutical regulatory environment is very clear to industries
and having clear guidelines for drugs regulation. And USAs market is very favorable for market
because it promotes the research and very profitable for industries. There are a number of recent
regulatory challenges of regulatory agency USFDA for e.g. DMF completeness, GDUFA
(Generic Drug User Fee), change in Stability Requirement, Batch requirement , QbD (Quality by
Design) concept, SPL , QOS (Quality overall Summary) , Review clock concept, Stringent
regulation of RTF/Screening. Attempts have been made in this article to study the positive and
negative impact of these challenges on pharmaceuticals. So by analyzing this organization can
decide on tailor made their organization strategy to get success in USA.

August 22, 2014


MIS-5

ANTIOXIDANTS: THE AGING DETERMINANTS

Babita Saroha
1
, Dinesh Kumar
1
, Usha Bhocal
1
, Sunita
2
, Veer Bhan
Research Scholar, Drosophila Research Laboratory, University Institute of Engineering and
Technology, MDU Rohtak-124001
Assistant Professor, Pt NRS Govt. College Rohtak, MDU Rohtak-124001
Assistant Professor, University Institute of Engineering and Technology, MDU Rohtak-124001
Email: saroha83@gmail.com

Abstract
Aging is characterized by a progressive decline in the efficiency of physiological function and by
the increased susceptibility to disease and ultimately death. Damage to cells caused by free
radicals is believed toplay a central role in the aging process and in disease progression.
Antioxidants are our first line of defense against free radical damage, and are critical for
maintaining optimum health and comfort. The need for antioxidants becomes even more
significant with increased exposure to free radicals. Pollution, cigarette smoke, drugs, illness,
stress, and even exercise can increase free radical exposure. Because so many factors can
contribute to oxidative stress, individual estimation of susceptibility becomes important. Many
experts believe that the diet with specific antioxidants may be significant in some instances and
the need of them may give valuable results. As part of a healthy lifestyle and a well-balanced,
wholesome diet with antioxidant supplementation is now being recognized as an important
means of improving free radical protection and ultimately healthy life-span.

MIS-7

REGULATION OF MEDICAL DEVICES IN INDIA

Dimple & Harish Dureja
Department of Pharmaceutical Sciences, MDU, Rohtak
Email:dimple.chaudhary009@gmail.com

Abstract
Medical devices are now a pervasive part of modern medical care. They are in many cases
associated with quality of care. In some cases, the use of devices has certainly improved quality.
In other cases, devices have been associated with many problems. The approach to quality of
devices has depended largely on regulation .Recently introduced guidelines and the amendment
in the Drug & Cosmetic Act will provide adequate guidance for both the manufacturers and
competent authorities to manage cases efficiently and appropriately. While these regulations and
reforms promise to clarify, unify, and expedite the process of manufacturing and importing
medical devices into India. Understanding the regulatory reforms imminent in India will be
crucial for foreign companies looking to enter or expand their business in India's medical
August 22, 2014


markets. It is hoped that the guidelines are implemented and regulated properly with effective
outcome.

MIS-8

INDIA- GRAPPLING WITH CONTROL OF DRUG PRICES

Prerna Kaushik &Dr. Harish Dureja
Deptt. Of Pharmaceutical sciences ,M.D. University, Rohtak
Email :- Pkaushik184@gmail.com

Abstract:
The prices of drugs in India are controlled by the DPCO 1955 which empowers the fixation and
regulations of prices of essential bulk drugs & their formulations that contribute to the need of a
mass of population. Price regulation started from 1970 where price regulation depends on the
fact that a companys pre-tax profit from its pharma business should not exceed 15%of its
pharma sales. In 1974, Hathi committee report was submitted to enquire into the conditions
prevailing in the pharmaceutical sphere of the country. Most recently, in 2013 DPCO came
which focuses on various key principles :-Essentiality of drugs ,Control of formulation prices
only ,Market based pricing (data available with IMS health),Previously only 74 drugs were under
the price control but at present all the 348 drugs under NLEM-2011 are under price control so
that the life saving drugs can be available at affordable equitable basis.

MIS-9

INNOVATIONS FROM NATURE A NEW COMMENCE

Kaushik Vichitra
1
, Chaudhary G.D.
2
,Ahmad Shoaib
2
and SainiVipin
1
1
MM College of Pharmacy, MM University, Mullana, Ambala, Haryana
2
Rayat&Bahra Institute of Pharmacy, Sahauran, Mohali, Punjab
Email: vichitrakaushik@rediffmail.com

Abstract
Natural Products are very valuable pharmaceutical leads due to their potency against often
difficult targets, but they can suffer from issues such as poor Pharmacokinetics, and their
intractability to synthetic chemistry has hindered their use.There is a current trend of discovery
and applications of bioactive agents from natural sources. Natural products can have very high
affinity to the target that they are naturally evolved to bind, very distinct from low-affinity
synthetic fragments. Furthermore, Natural products seem to be able to address a different range
of targets from combinatorial chemistry-derived small molecules. At present, compound libraries
from combinatorial chemistry are the major source for high throughput screening programs in
drug discovery. On the other hand, nature has been proven to be an outstanding source for new
and innovative drugs. Recent advances in the applications of various hyphenated techniques,
August 22, 2014


e.g., GCMS, LC-PDA, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, and CE-MS, in the context
of preisolation analyses of crude extracts or fraction from various natural sources, isolation and
on-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality
control of herbal products, dereplication of natural products, and metabolomic studies were used.
Nature represents an extraordinary reservoir of novel molecules and there is currently a
resurgence of interest in natural products as a possible source of new lead compounds for
introduction into therapeutical screening programmes.To realize that utility and to share in the
benefits, organizations should be prepared not only to participate actively in the discovery
process, but also to share the financial risks.

MIS-10

DOCTOR OF PHARMACY EDUCATION IN INDIA- A CRI TI CAL STUDY

A. Pandurangan, Amandeep Kaur*, Diksha Sharma, Vipin Saini
M.M. College of Pharmacy, M.M. University, Mullana, Ambala, Haryana, India-133207

Abstract:
The past few decades witnessed many scientific developments and achievements in the areas of
Hospital, Clinical and Community pharmacy services throughout the world. The Doctor of
Pharmacy (Pharm. D) degree is a professional doctor degree in Pharmacy focusing on hospital,
community and clinical pharmacy activities. It is a global program in pharmacy education and
is very much similar to professional degrees like Doctor of Medicine (MD) in USA. The
Pharmacy council of India (PCI) managed to introduce a six year regular Pharm. D and the
three year post baccalaureate Pharm. D in 2008 in the country through a Gazette notification of
Government of India dated 16th May 2008. By 2013 November, the PCI had given approval to
over 140 institutions covering states like Kerala, Tamil Nadu, Karnataka, Andhra Pradesh,
Maharashtra, Gujarat, Rajasthan, UP and Punjab for starting Pharm. D in India. The first batch
of regular Pharm. D will graduate by the end of 2014.The 6 year Pharm. D program in India
should help to establish an effective and trustworthy relationship between the pharmacy practice
department and the health care professionals in the hospitals. The Pharm. D students have to
exhibit their caliber, competence and capabilities in making the drug therapy and health care
safer, cost-effective and user friendly. The strengths, weakness, opportunities and threats
(SWOT) of Indian Pharm. D are critically and scientifically analyzed and evaluated in this study
paper.

August 22, 2014


MIS-11

BIOMARKERS: AN EMERGING TOOL IN MEDICAL PRACTICE

Deepa Batra
*1
, Swati Aggarwal
1
, Vaibhav Gupta
1
, Kunwar Singh
1
1. Doon Valley Institute of Pharmacy & Medicine, Karnal, Haryana

Abstract
Biomarkers have gained immense scientic outlook and clinical value in the practice of modern
medicine. Biomarker indicates potentially useful signals along the whole spectrum of the disease.
Before diagnosis, these markers could be used for screening and risk assessment. During
diagnosis, markers can determine staging, grading, and selection of initial therapy. During
treatment, they can be used to monitor therapy, select additional therapy, or monitor recurrent
diseases. Biomarkers have many potential applications in oncology, Alzheimer disease and
cardio vascular diseases whose clinical diagnosis is inaccurate even among experienced
investigators. They are becoming an essential part of clinical development because they offer a
faster alternative to the conventional drug development approach and the promise of safer drugs
in greater numbers, approved more quickly. Advances in genomics, proteomics and molecular
pathology have generated many candidate biomarkers with potential clinical value. In the future,
integration of biomarkers, identied using emerging high-throughput technologies into medical
practice will be necessary to achieve personalization of treatment and disease prevention

.MIS-12

QUALITY BY DESIGN

Monika Punia
1
, Kapil Joshi
2
, Harish Dureja
1
1 Faculty of Pharmaceutical Sciences, M. D University, Rohtak-124001
2Quality Assurance, Dr. Reddys Laboratories Limited, Baddi-173205

Absract
Quality by design is modern approach to obtain the high level of assurance of the product, for
increasing the efficiency of manufacturing product by reducing manufacturing cost and product
rejection, the ultimate goal of this is to design robust process. QbD is a scientific, risk based,
holistic and proactive approach to pharmaceutical development. The concept promote industrys
understanding of the product and manufacturing process starting with product development,
basically building quality in, not testing it. Testing of Products confirms the product quality.
Implementation of QbD will enable transformation of the chemistry, manufacturing, and controls
(CMC) review of abbreviated new drug applications (ANDAs) into a science-based
pharmaceutical quality assessment. Under this concept of QbD during designing and
development of a product, a company needs to define desire product performance Target product
Profile (TPP), and identify critical quality attributed (CQA).It is a new approach to drug
development could increase efficiencies, provide regulatory relief and flexibility, and offer
important business benefits throughout the products life cycle.
August 22, 2014


MIS-13

EBOLA VIRUS: A DEADLY MENACE

Mohit Madan *A.C.Rana * Dhirender Kaushik *Manjusha Chaudhary
Institute Of Pharmaceutical Sciences Kurukshetra University, Kurukshetra

ABSTRACT
Ebola virus disease (EVD), formerly known as Ebola haemorrhagic fever, is a severe, often
fatal illness in humans. EVD outbreak have a case fatality rate of up to 90%.Ebola first
appeared in 1976 in 2 simultaneous outbreaks, in Nzara, Sudan. The latter was in a village
situated near the Ebola River, from which the disease takes its name. Genus Ebolavirus is 1 of
the 3 members of the Filoviridae family.GenusEbolavirus comprises of 5 distinct speciesin
which 3 are deadly virus and 2 are not. Ebola is introduced into the human body through close
contact with the blood, secretions, organs or other bodily fluids of infected animals. In Africa,
infection has been documented through the handling of infected chimpanzees, gorillas, fruit
bats, monkeys. EVD is a severe acute viral illness often characterized by the sudden onset of
fever, intense weakness, muscle pain, headache and sore throat. This is followed by vomiting,
diarrhoea, rash, impaired kidney and liver function, and in some cases, both internal and
external bleeding. Ebola virus infections can be diagnosed effectively in a clinical or
pathological laboratory through several tests: Antibody-capture enzyme-linked
immunosorbent assay (ELISA), Antigen detection tests, Serum neutralization test, Electron
microscopy, Virusisolation by cell culture. No licensed vaccine for EVD is still available.
Several vaccines are being tested, but not any vaccine is available for clinical use.

MIS-15

PARAMOUNT PART OF LIFE: SLEEP

Garima*, Dhirender Kaushik, Manjusha Chaudhary
Institute of Pharmaceutical Sciences Kurukshetra University, Kurukshetra

Abstract
Sleep and wakefulness are linked in part to the activity of the circadian clock. Loss of sleep
creates an overwhelming and uncontrollable need to sleep and effects virtually all physiological
functions .Sleep is not something to fill time when person is inactive. Sleep is required activity
not an option. Sleep is not being seen as potential risk factor of obesity along with the two most
commonly identified risk factors: lack of exercise and overeating.Lack of sleep also may lead to
microsleep. Microsleep refers to brief moments of sleep that occur when you're normally awake.
Lack of sleep leads type 2 diabetes. There is also growing evidence of a connection between
obstructive sleep and apnoea and heart disease .Some other side effects like obstructive sleep
restless leg syndrome, narcolepsy parasomanias are also observed.

August 22, 2014


MIS-16

PHARMACOVIGILANCE: A WAY FOR BETTER TOMARROW

Karishma*, Vipul, SachchidanandPathak and Himmat Singh
Suresh GyanVihar School of Pharmacy - Jaipur

Abstract
Pharmacoepidemiology is the study of the use and effects of drug in human populations and
Pharmacovigilance is the branch of pharmacoepidemiology. Pharmacovigilance is defined by the
WHO as the post approval scientific and data gathering activities related to the detection,
assessment, understanding and prevention of adverse events or other drug-related problems.
The thalidomide tragedy of 1960s opened the eyes of drug regulators as well as other concern
body to establish a way to ensure drug safety. Appropriate and effective monitoring of ADRs,
i.e. pharmacovigilance, is the only best way to safeguard the public health. Spontaneous
reporting system (SRS) is the first and most widely used method to report ADRs in spite of
under-reporting as a major limitation. It is enable to early detection of new, rear and serious
ADRs. All drugs have side effects and to monitor their side effect, and collect data in a
systematic way and analyse it to reach a meaningful conclusion, is the basis of the
pharmacovigilance programme in India. The National Pharmacovigilance Programme (NPVP)
was launched in India in 2004 with World Bank funding till 2009. Hence the need for
pharmacovigilance arose since man started using medicines. The ultimate goal of
pharmacovigilance is to faster the rational and safe use of medicines and to identify new
information about hazards associated with medicine and Prevent harm to patient and to improve
patient care and safety in relation to the use of medicines and all medical and paramedical
intervention.

MIS-17

WHAT, WHEN AND HOW OF THE PROMOTION OF RATIONAL USE OF
MEDICINE

SweetyHooda
1
, Ritu Gilhotra
2
and Neeraj Gilhotra
3
*

1
Pharmacology Laboratory,Department of Pharmaceutical Sciences, MaharshiDayanand
University, Rohtak 124 001, Haryana, India
2
School of Pharmacy, GyanVihar University, Jaipur-302 025, Rajasthan, India
*
3
University, Rohtak 124001, Haryana, India

Abstract
A growing number of pharmaceuticals are available on the world market. There has ever
increasing consumption of drugs and thus out of pocket expenditure on them. In spite of this,
almost every medicine consumer is equally hesitant and ignorant regarding aspects of rational
medicine use such as economic purchase of medicine as well as rational medicine consumption.
The paper aims at disseminating the historical perspectives, need, rise of demand, practices, key
August 22, 2014


elements and possible role players in South East Asian Countries. Different qualitative and
quantitative approaches to promote practice of rational use of medicines and information,
education and intervention methodologies are presented.

MIS-18

AVAILABILITY AND RELATIVE PRICE PAID FOR BRANDED MEDICINES FOR
COMMON AILMENTS IN TEN AREAS (100 PHARMACY OUTLETS) OF SONEPAT
DISTRICT

Anjali Goyal
1
, Ritu Gilhotra
2
and Neeraj Gilhotra
3
*

1
2
School of Pharmacy, GyanVihar University, Jaipur-302 025, Rajasthan, India
*
3
Abstract
Essential medicines satisfy the priority health care needs, selected with regard to public health
relevance and comparative cost-effectiveness, intended to be available at all times in adequate
amounts and at a price the individual and the community can afford. Up to 90% of the population
of developing countries purchases medicines through out-of-pocket payments, making medicines
the largest family expenditure item after food. The present study aimed to collect basal data on
availability, relative price paid for these medicines in selected areas of Sonepat District. Data
collector visited these medicine outlets and recorded medicine availability and price using a
standardized format. For each medicine, data are collected for brand name (s), available for
common ailments. Price of these medicines was compared with lowest as well as highest brand
price sold for respective ailment in the market. The results were interesting and projected a
positive picture of availability of almost all selected medicines at selected tenareas and one
hundred retail medicine outlets of Sonepat District.The study helped to provide baseline data on
medicine sales practices of selected retail pharmacy outlets of Sonepat District. A significantly
lower medicine price was observed in area 7 out of all the ten areas chosen for the present study.

August 22, 2014


MIS-19

DRUG SAFETY RISK MANAGEMENT PLAN

Vipin Saini*, Akash Jain and Jasmine
M.M. College of Pharmacy, Mullana, Ambala
Vipinsaini31@rediffmail.com

Abstract
Drug Safety Risk Management Plan (DSRMP) is a regulatory document submitted to health
authorities with an application for a new marketing authorization, with Periodic Safety Update
Reports(PSUR), as a standalone document. Once the DSRMP is accepted by the Health
Authorities, the Market Authorization Holder (MAH) has a legal obligation to perform the
activities described in the DSRMP.DSRMP is prepared at the time of a request for approval of a
new drug, new indication, new patient population, etc. or it has to be submitted with submission
dossier and upon identifying a significant new safety concern at the request of health
authorities.The objectives of DSRMPis to specify what is and is not known about safety of a
drug at the time of submission (Safety Specification Safety Specification), to further characterize
the safety risks post authorization (Pharmacovigilance Plan) and where necessary, to define
appropriate measures to minimize known risks to patients.

MIS-20

RISK OF SELF MEDICATION

Garima Kumari,* DR RituGilhotra *DeepikaDeopa
Email : garimakumari477@gmail.com
(Suresh GyanVihar University) jaipur,

Abstract
Self-Medication is The Selection And Use Of Medicines By Individuals (Or A Member Of The
Individuals' Family) To Treat Self-Recognized Or Self-Diagnosed Conditions Or Symptoms
Like Fever, Pain Etc. Some Of The Several Benefits Have Been Linked To Appropriate Self-
Medication, Some Of Them: Increased Or Access To Medication And Relief For The Patient,
The Main And Active Role Of The Patient In His Or Her Own Health Care, Better Use Of
Physicians And Pharmacists Skills And Reduced (Or At Least Optimized) Burden Of
Governments Due To Health Expenditure Linked To The Treatment Of Minor Health Conditions
However, In Many Cases Self-Medication Is Far From Being A Completely Safe Practice, In
Particular In The Case Of Non-Responsible Self-Medication. Some Potential Risks Of Self-
Medication Practices Include: Incorrect Self-Diagnosis, Delays In Taking Medical Advice When
Needed, Infrequent But Severe Adverse Reactions, Most Dangerous Drug Interactions, Wrong
Manner Of Administration, Incorrect Dosage, Improper Therapy, Masking Of A Severe Disease
And Risk Of Dependence And Abuse. In This Short Review We Can Analyze The Problem
Related To Self Medication Practises Particularly: Polypharmacy And Drug Interactions,
August 22, 2014


Medications Abuse Or Dependence (Tolerance Level Inc), Misdiagnosis And Incorrect Choice
Of Treatment.

MIS-21

PHARMACOGENOMICS- A NEED OF THE HOUR

Aditya Delu
1
, Reeta Sethi
2
, Jagtar Singh
1
and Yash Paul
1

Lord Shiva College of Pharmacy, Sirsa
1

DDR college of Pharmacy, Khandewla, Gurgaon
2

Abstract
Pharmacogenomics correlates the Pharmacokinetics and Pharmacodynamics of administered
drug molecules with genetic inheritance of individuals, which is possibly thought to be one of the
important factors leading to variability in response of drug irrespective of its bioavailability. The
term has its origins in Pharmacology and genomics, incorporating genetically monitored
regulation of Pharmaceuticals on administration to patients.
Every Year, a large number of patients die of Adverse Reactions to drugs and more than twice
these are Hospitalized. Application of Pharmacogenomics will predict who's likely to have a
negative or positive reaction to a drug and thus it improves drug choices. Testing of genomic
variations improves determination of correct dose for each individual thus leading to safer dosing
options. Pharmacogenomics leads to improvements in drug development, thus permitting
Pharmaceutical companies to determine effectiveness of new drugs in different groups of
populations. Utilizing the concept of Pharmacogenomics would reduce the number of deaths as
well as hospitalization cases owing to adverse drug reactions. This would avoid purchase of
ineffective drugs for individuals on the basis of genetic variation. Moreover, use of this concept
would speed up the clinical trials for new drugs.
At present there is a strong need of spreading the concept of Pharmacogenomics among
Pharmaceutical teachers through changes in syllabi they teach and also Pharmaceutical scientists
through research project allotments focused on Pharmacogenomics. This integrated step and its
outcomes would help in eliminating chances of adverse effects on human body, ensuring the
safety and effectiveness of personalized medicines.

August 22, 2014


MIS-22

ANTIDOTE FOR OPIUM POISONING

Anjali , Dr. A.C.Rana , Dr. Dhirender Kaushik , Mrs. Manjusha Chaudhary
Institute Of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra
arbhardwajpr@gmail.com

Abstract
Opoids are the commonly misused substances by drug abusers around the world. Opoids come in
various forms like; Morphine, Codeine, Pethidine, Dihydrocodeine. Opids are a class of drugs
derived from the extracts of plantOpium poppy (from the dried latex of plant Papever
somniferum). Opium contains approximately 12% of analgesic alkaloid Morphine, which is
processed chemically to produce Heroin and other synthetic opoids for medical use and for
illegal drug trade. Pharmacologically they are used as analgesics but they give a feeling of
euphoria and a degree of sedation. These side effects are the cause of Abuse of opoids . An opoid
overdose is an acute condition due to excessive use of narcotics. The Administration routes are
Oral, Inhalational (smoking), Intramuscular, Intravenous ,Subcutaneous ,I ntrathecal . Sign and
symptoms of Opium Poisoning is i.e. Decrease level of consciousness (calm the patient),
Decrease pinpoint pupil, Respiratory depression, Decrease in heart rate, Blue lips and nails are
caused by insufficient oxygen in the blood, Seizures, Muscle spasms. Antidote for Opium
Poisoning are, Potassium Permanganate was discovered by Dr. Moor as an antidote for opium
poisoning. Gastric Lavage treatment is used to remove unabsorbed drug (poison) by using
antidote. Naloxone and its variant Naltrexone was used for treating opium poisoning. It primarily
meant to treat opoid and alcohol dependence. Naloxone dose was given 0.4-0.8 mg IV
(intravenously) for adult and 0.01 mg for children .This antidote was repeated in two minutes
until pupil dilates and respiration picks up. In earlier times Coffee was used as only antidote for
opium poisoning.

MIS-23

EVOLUTION OF PHARMACY PRACTICE

Gaganpreet Kaur
Guru Gobind Singh College Of Pharmacy, Yamuna Nagar
E-mail: gaganpreet_pharmacy@yahoo.com

Abstract
The Practice of Pharmacy has been undergoing dramatic changes in recent years, in a number of
areas. A main trend has been the need to modernize and automate the production, packaging,
distribution, and provision of drugs, particularly of prescription drugs, which have to be
monitored and secured at all times. Pharmacy Practice concern the proliferation of new and
exciting areas of practice, the effects of e-commerce on the profession and the emergence of
virtual pharmacies, the use of automated technology and technicians for dispensing functions and
August 22, 2014


shifts in the pharmacist labor supply.Thedynamic evolution of pharmacy practice, as seen
through the eyes of new professional students, encompasses the need for improvements in
medication safety access to pharmaceutical care, reducing the incidence of adverse drug events,
enhancing cultural competence in the deliver of care, incorporating the prudent use of technology
and supportive personnel, the acquisition of advanced training for delivering pharmaceutical
care, involvement in disease state management, and the delivery of medication therapy
management services.

MIS-23

NOSOCOMIAL DISEASES OR HOSPITAL ACQUIRED DISEASES

Bhumika Gupta
Guru Gobind Singh College of Pharmacy, Yamuna Nagar
Email id: bhumika.gupta30@gmail.com

Abstract:
The Nosocomial diseases may be regarded as the ones which originate from the hospital,
acquired in a hospital, especially in reference to infection. The term "nosocomial" comes from
two Greek words: "nosus" meaning "disease" + "komeion" meaning "to take care of." Hence,
"nosocomial" should apply to any disease contracted by a patient while under medical care.
However, common usage of the term "nosocomial" is now synonymous with hospital-acquired.
Nosocomial infections are infections that have been caught in a hospital and are potentially
caused by organisms that are resistant to antibiotics. A bacterium named Clostridium difficile is
now recognized as the chief cause of nosocomial diarrohea in the US and Europe. Methicillin-
resistant Staphylococcus aureus (MRSA) is a type of bacteria that is resistant to certain
antibiotics and may be
acquired during hospitalization. In the United States, the Centers for Disease Control and
Prevention estimated roughly 1.7million hospital-associated infections, from all types of
microorganisms, including bacteria, cause or contribute to 99,000 deaths each year. In hospitals
staff themselves act as a vector in transferring the infections to either the in patients and out
patients who come for treatment as well. Various modes of transmission of infection may
include: contact transmission, droplet transmission, airbone transmission and common vehicle
transmission. The nosocomial diseases may be prevented by various methods such as:
Monitoring the proper hygiene of the hospital premises as well the quality air inlet, Proper
sanitation as well the clean uniforms of the staff including gloves, aprons etc., Alcohol rubs may
also be used, the waste of the hospital must be disposed off properly, Isolation of the severely
wounded patients must be maintained such as burnt cases and severely infected patients.

August 22, 2014


MIS-25

SPECIFIC ABSORPTION RATE VARIATION IN BRAIN PHANTOM DUE TO
EXPOSURE BY A 3G MOBILE PHONE

Vandana
Guru Gobind Singh College of Pharmacy, Yamunanagar
Email: - vandanarajak61@gmail.com

Abstract:
3G mobile phone frequency (1718.5MHz) and the emitted radiation directed toward brain
phantom. The induced fields in the phantom material are measured. Set up to lift the plane
carrying the mobile phone is run by a pulley whose motion is controlled by a stepper motor.
Binaural beats may influence functions of the brain in ways of those related to hearing. This
phenomenon is called "frequency following response". The concept is that if one receives a
stimulus with a frequency in the range of brain waves. Human hearing is limited to the range of
frequencies from 20 Hz to 20,000 Hz, but the frequencies of human brain waves are below about
40 Hz.Beat frequencies of 40 Hz have been produced in the brain with binaural sound and
measured experimentally. International Agency for Research on Cancer(IARC) classified mobile
phone radiation as Group 2B - possibly carcinogenic (not Group 2A - probably carcinogenic)
that means that there "could be some risk" of carcinogenicity. The WHO added that "to date, no
adverse health effects have been established as being caused by mobile phone use. Mobile phone
causes many effect shows on brain .It found a leakage of albumin into the brain via a
permeated bloodbrain barrier. Glioma or meningioma was observed with use of mobile phones.
There were suggestions of an increased risk of glioma at the highest exposure levels, but biases
and error prevent a causal interpretation. It is concluded that SAR values are sensitive to the
angular position of the moving platform and are well below the safety criteria prescribed for
human exposure.

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