THE FEASEBILITY STUDY OF EXTRACTION OF NICOTINE FROM TOBACCO WASTE
BY CHATIKOBO ELISON H1210038C CHIDEMBESE ASSURED H1214231J CHIGWANDA CLOUD H1110735X CHIRAMBA GEORGE H1213013N DAMBANEMWEYA KUPAKWASHE H1210050J DHLAMINI SHAMISO H1210052Q
SUPERVISED BY
MRS TSORAI MR MUTADZA
THIS RESEARCH AND DEVELOPMENT WAS SUBMITTED TO HARARE INSTITUTE OF TECHNOLOGY IN PARTIAL FULFILMENT OF THE BACHELOR OF TECHNOLOGY (HONOURS) DEGREE IN CHEMICAL AND PROCESS SYSTEMS ENGINEERING
2014 i
Copyright
All rights reserved. No part of this project may be reproduced, stored in any retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise for scholarly purpose, without the prior written permission of the author and of the Harare Institute of Technology on behalf of the authors.
ii
Declaration We as a group hereby declare that this work has not previously been accepted in substance for any degree and is not being concurrently submitted in candidature for any degree.
Students Signature: . Date . (Chatikobo Elison) Students Signature: . Date . (Chidembese Assured) Students Signature: . Date . (Chigwanda Cloud) Students Signature: . Date . (Chiramba George) Students Signature: . Date . (Dambanemweya Kupakwashe) Students Signature: . Date . (Dhlamini Shamiso)
Supervisors Signature: .. Date . (Mrs Tsorai) Supervisors Signature: .. Date . (Mr Mutadza)
iii
Dedication Firstly, we would like to dedicate this piece of work to our late group member Mr Tafadzwa J Danda, our families for the love, care and support they have given to us and also to our department and our university for the knowledge we have attained.
iv
Acknowledgements Firstly, we would like to thank the Lord God almighty for his mercies and for seeing us through in this research and throughout our life. Secondly, we would like to thank our supervisors Mrs Tsorai and Mr Mutadza for their guidance and the time they set aside to supervise us, through which we managed to come up with the project. Thirdly we would like to thank our parents for their encouragement and support. Special appreciation goes to all our friends for the encouragement and technical support. Lastly, our sincere gratitude goes to all lecturers in the Chemical and Process Systems Engineering Department, without them it would have been impossible for us to acquire the knowledge and be able to undertake this project.
v
Table of Contents Copyright .................................................................................................................................................. i Declaration .............................................................................................................................................. ii Dedication .............................................................................................................................................. iii Acknowledgements ................................................................................................................................ iv List of Tables ......................................................................................................................................... vii List of Figures ........................................................................................................................................ vii Abstract ................................................................................................................................................ viii CHAPTER 1: INTRODUCTION ................................................................................................................... 1 1.2 Problem Statement ........................................................................................................................ 1 1.2.1 Limitations .............................................................................................................................. 1 1.2.2 Delimitations .......................................................................................................................... 1 1.3 Aim ................................................................................................................................................ 2 1.4 Objectives...................................................................................................................................... 2 CHAPTER 2: LITERATURE REVIEW ........................................................................................................... 3 2.1 Physical and Chemical Properties of Nicotine .............................................................................. 3 2.2 Factors Affecting Nicotine Content in Tobacco ............................................................................ 5 2.3 Uses of Nicotine ............................................................................................................................ 6 2.4 Healthy effects of Tobacco/ Nicotine ........................................................................................... 6 2.5 Toxicology ..................................................................................................................................... 7 2.6 Past Researches ............................................................................................................................ 8 2.7 The cold infusion process .............................................................................................................. 8 CHAPTER 3: RESEARCH METHODOLOGY .............................................................................................. 10 3.1.2 Objectives: ........................................................................................................................... 10 3.1.3 Apparatus: ............................................................................................................................ 10 3.1.4 Chemicals ........................................................................................................................... 11 3.2 Extraction of nicotine .................................................................................................................. 11 3.3 Testing for the presence of nicotine ........................................................................................... 11 3.4 Separation using simple distillation ............................................................................................ 12 3.5 Determining Nicotine Concentration .......................................................................................... 12 3.6 Material and Energy balances ..................................................................................................... 13 CHAPTER 4: RESULTS AND ANALYSIS .................................................................................................... 14 4.1 Extraction of nicotine .................................................................................................................. 14 4.1.1 Conclusion for extraction of nicotine ................................................................................... 14 vi
4.1.2 Testing for the presence of nicotine .................................................................................... 14 4.1.3 Conclusion for testing for the presence if nicotine .............................................................. 14 4.2 Analysis for testing the presence of nicotine .......................................................................... 15 4.3 Conclusion ................................................................................................................................... 15 CHAPTER 5.0 PROCESS DESIGN ............................................................................................................. 16 5.1 Mass balance at laboratory scale ................................................................................................ 18 5.2 Energy Balance For The Extraction Process ................................................................................ 19 CHAPTER 6.0 ECONOMIC ANALYSIS ...................................................................................................... 21 6.1 Equipment ................................................................................................................................... 21 6.2 Scaling to industrial level ............................................................................................................ 21 6.3 Profit Analysis.................................................................................................................................. 23 6.4 Return on investment (ROI) ........................................................................................................ 23 6.5 Break Even Analysis..................................................................................................................... 24 CHAPTER 7: CONCLUSION AND RECOMMENDATIONS ......................................................................... 25 7.1 Conclusion ............................................................................................................................. 25 7.2 Recommendations ...................................................................................................................... 25 APPENDIX .............................................................................................................................................. 26 A1 Gantt Chart .................................................................................................................................. 26 A 2 Cash Flow Forecast ..................................................................................................................... 27
vii
List of Tables
Table 2.1 Zimbabwe tobacco production area, yield and output, 2010-2013..3 Table 2.2 Physical properties of nicotine....4 Table 6.1 Direct costs....22 Table 6.2 Indirect costs..22 Table 6.3 Plant Overheads.22 Table 6.4 General Expenses..23
List of Figures
Figure 2.1 Structure of nicotine compound ..........14 Fig 4.1 Titration experiment results..23 Fig 5.1 Block Flow Diagram for the extraction process...25 Fig 5.2 Process Flow Diagram for the extraction process25 Fig 5.3 Mass Balance Diagram27
viii
Abstract Literature reflects that nicotine is in abundance in tobacco waste. A number of processes have been studied for the removal of toxic or otherwise offending substances from tobacco and stimulants. Unfortunately, the process of extracting nicotine from tobacco with solvents such as ammonia, ethylene oxide and similar processes have not attained general acceptance due to the fact that the extraction solvents are not sufficiently selective and remove not only the nicotine, but varying amounts of the desirable constituents. For a long time effective extraction of nicotine has remained elusive due to complications associated with selectivity. The use of condensed gases has been studied quite extensively because of the known selective dissolving capacity of this substituent .However, this extraction solvent has not attained any appreciable acceptance due to the fact that SO 2 is not sufficiently selective, generates toxic residues and is highly corrosive to the apparatus employed. Based on this scientific understanding, this research was carried out with a view to improvise an innovative a selective extraction of nicotine from tobacco with nontoxic solvents which do not corrode the apparatus employed. An iterative approach was employed in which distilled water was used for the extraction of nicotine from tobacco. Experiments were carried out for the extraction, verification and determination of the percentage purity of nicotine. The percentage purity of nicotine obtained was 95%. The process equipment used included; crusher, mixing column, filtration column, distillation column and condenser. The proposed extraction method was carried out with low-boiling point organic solvents at low temperature resulting in lower consumption of chemicals and shorter time required for the process. This ensured added purity of the target product and elimination of the need for high pressure in the technological process. Economically the project has got a favorable return on investment of 52.6%, a payback to investors of 1.9 years and break-even point is at 6.9 % of the total capacity.
1
CHAPTER 1: INTRODUCTION From harvesting of Tobacco to manufacture of product, large quantities of waste material comprising rejected leaves, broken bits of lamina, midribs stalks & stems accumulate. Nicotine is by far the most important by-product derived from tobacco waste. The process relates to methods of nicotine extraction from tobacco waste. The proposed method for extraction of nicotine from tobacco waste implies a continuous extraction from raw material with distilled water at room temperature (25 0 C) followed by recurrence for further reuse in the process. The technological advantages are the opportunity to use both primary raw materials and production wastes, shorter time required for extraction, cost reduction and process simplification for extraction of nicotine, reduction of concomitant substances in the target product, and no need in using acids. These advantages are achieved by the extraction method with distilled water which results in lower consumption of chemicals, lower temperature, little time required for the process and additional purification of the target product and eliminate needs for high pressure to be used in technological process. 1.2 Problem Statement
Literature reflects that nicotine is in abundance in tobacco waste. When tobacco is harvested there is waste which can be made to good use. Unlike using the waste in making manure; the waste can be used in nicotine extraction. This is a major advantage because the nicotine can be used in making important products such as pharmaceutical drugs. The other advantages are: 1) High concentration of nicotine in the extract. 2) Making use of waste products as raw materials to produce products of higher value. 3) Runaway from the use of chemicals like tartaric acid. 1.2.1 Limitations 1) Takes slightly longer to extract. 2) There is need for safety measures to be undertaken. 1.2.2 Delimitations The project just focuses on the technological and economic feasibility study of extraction of nicotine from tobacco waste using distilled water. 2
1.3 Aim The technical and economic feasibility study for the extraction of nicotine from tobacco waste. 1.4 Objectives 1) To determine the technological process for the extraction of nicotine from tobacco waste. 2) To provide a shorter, less expensive and more efficient process of nicotine extraction. 3) To evaluate the yield, processing time required and solvent usage of different extraction approaches. 4) To determine if it is economically feasible to extract nicotine from tobacco waste.
3
CHAPTER 2: LITERATURE REVIEW
Tobacco waste is composed of mineral matter and organic compounds. Alkaloids in tobacco leaves make it suitable to be used by human beings. Alkaloids are a group of nitrogen- containing bases. (Vernellia R. Randall. 1999. History of Tobacco,) among the alkaloids, nicotine is recognized as an essential component in tobacco cultivation. On the contrary the agricultural sector for Zimbabwe, being the backbone of the economy underpinning economic growth, food security and poverty eradication, continues to experience severe systemic challenges within its entire value chain ranging from lack of agricultural financing to lack of affordable inputs. This has also been exacerbated by prolonged periods of drought caused by climatic changes and yet the waste is not being put into economic use. In the tobacco industry approximately one tenth of the tobacco yield is produced as waste. The relationship between the area harvested, tobacco yield, tobacco production and waste yield is shown in table 2.1. Table 2.1 Zimbabwe tobacco production area, yield and output, 2010-2013 Year Area harvested(ha) Tobacco Yield(kg/ha) Tobacco Production(tone) Waste yield(kg/ha) 2010 99000 2626.3 260004 262.6 2011 88 286 2188.2 193187 218.9 2012 90 769 2508.9 227730 251.0 2013 91 851 2611,5 239869 261.1 Source: TPZ (Tobacco Processors of Zimbabwe) (National Tobacco Enterprise Co. zw, 2012 (1998 E.C.),) 2.1 Physical and Chemical Properties of Nicotine Nicotine is also called 3-(1-methyl-2-pyrrolidinyl) pyridine according to the IUPAC Nomenclature. It is a bicyclic compound with a pyridine cycle and a pyrolidine cycle. The molecule possesses an asymmetric carbon and so exists in two enantiomeric compounds.
4
Figure 2.1: The Structure of nicotine compound In nature, nicotine only exists in the S shape, which is laevogyrate. (Compton Mackenzie, 1957.Sublime Tobacco). Some physical properties of Nicotine are listed in table 2.2.
Table 2.2: Physical properties of nicotine
Chemical structure C 10 H 14 N 2
Molecular weight 126.26g/mol Melting point -79 C Boiling point 247 C Density d = 1.00925g/cm 3 Refractive Index n = 1.530 Vapour Pressure 0.006 kPa at 25 C Viscosity 2.7 mPa s at 25 C 1.6 mPa s at 50 C Surface tension 37.5 dynes/cm at 25.5 C 37.0 dynes/cm at 36.0 C Rotatory Index (S) D = -169.3 at 20 C
Vernellia, R. and Randall, T. (1999). History of Tobacco, Boston: Boston University.
Nicotine is a liquid alkaloid, pale yellow to dark brown oily liquid with a slight fishy when warm or unpleasant pungent odors with a sharp burning persistent bitter taste (Bulletin on Narcotics 1996). As a nitrogenous base, it is strongly alkaline in reaction and forms salts with acids and double salts with many metals and acids that are usually solid and water- soluble. Nicotine is hygroscopic; hence it is miscible with water below 60 C
5
2.2 Factors Affecting Nicotine Content in Tobacco
Nicotine level is a key index for evaluating the quality of tobacco, and is closely related to a lot of factors. Some of the factors affecting nicotine level of tobacco are the following:
Tobacco species or type: is the most determinant factor that affects nicotine level in tobacco. There are different tobacco species in the world which have different nicotine content. For instance, among the type of tobacco Maryland and Turkish (oriental) are low in nicotine; the flue-cured (Virginia), Burley, and Connecticut Cigar wrapper are medium; the Pennsylvania and N. rustica are high in nicotine level. (American Indian Culture and Research Journal, 1995, V. 19, N. 2, pp 143-64).
Soil type: is another factor that affects nicotine content. The quality of tobacco is highly correlated with the nicotine content and good quality tobacco requires fertile, well-drained, moist soil. Soil can affect leaf size, texture, and color. Sandy soils tend to produce a relatively large leaf that is light in color and body, fine in texture, and burns with a weak aroma. Heavier soils, which contain silt and clay, tend to produce a small, dark leaf with a heavy body and a strong aroma when burnt. (Tobacco, Culture, and Health among American Indians: A Historical Review). Moreover, the amount of root development as conditioned by depth of soil influences the total nicotine content of tobacco plant. Deep soil favors development of extensive root system, since nicotine elaboration primarily localized near the root tip then stored in the leaf and plants having the largest root system accordingly elaborate the largest total content of nicotine. On the contrary, damage of the root by any means reduces the nicotine content of the tobacco plant.
Nitrogen nutrition: is also related with soil type since almost all nitrogen consumed by the tobacco plant is obtained from the soil as ammonium and nitrate compounds. According to literature N is 17.3 % of the molecular weight of nicotine hence, the amount of nitrogen (N) supplied has direct relation with nicotine content of the tobacco plant (A Shude Unpublished, July 2006. pp 3) The nitrogen content of the soil also depends on the type of soil.
Topping height and time: also affect nicotine content in the tobacco plant, which is also related to nitrogen supply of tobacco plant. The reason is nitrogen absorbed after topping is effectively incorporated in to nicotine compared with nitrogen absorbed before topping. The 6
more nitrogen supply after removing the apex, results the higher the nicotine concentration in the leaves (Compton Mackenzie, 1957.Sublime Tobacco, London, Chatom and Windaus).Similarly, as the height of the position of topping increase, meaning large apex removed the thicker, darker and larger leaf produced as a result nicotine content of the tobacco plant become high.
Degree of maturity or ripening of tobacco: has also great effect on nicotine content of tobacco plant. As the tobacco plant maturity increases, it turns to yellow quicker, and quality index, with higher alkaloid (nicotine) and sugar content and less total nitrogen (National Tobacco Enterprise Sh.Co., brochure (Amharic version), 2005 (1998 E.C.)
Curing and yellowing period of tobacco: have also slight effect on the level of nicotine of the tobacco leaf that is extended yellowing period will increase level of nicotine of the tobacco leaf. But during aging and curing process of tobacco there is a marked decrease of nicotine with a concomitant increase of nicotine transformation products such as nicotinic acid, nicotine amide, oxy nicotine, cotinine and the like (A Shude Unpublished, July 2006. pp 3). 2.3 Uses of Nicotine Nicotine is most frequently encountered in tobacco products for smoking, chewing and sniffing and also as an adjunct to smoking cessation programs (gums, patches). Moreover, nicotine has found wide use as pesticides and insecticides. Dilute solutions are used on fruit and vegetables to control insects such as aphides, plant lice, whiteflies, leaf hoppers, etc. It may also be used to control certain animal skin parasites. For spray application, nicotine must be incorporated in a solution of soap or with emulsiable oil or must be mixed with hydrated lime or another powdered carrier to use as a dust (Research (EIAR) team visit report on tobacco production in Ethiopia and the constraints it has been facing). 2.4 Healthy effects of Tobacco/ Nicotine Tobacco is the leading cause of drug abuse related to mortality world over. Besides nicotine, tobacco consists of nearly 4000 substances including particles and gases, 1000 of them are produced during smoking. These include the known carcinogens and carbon monoxide which impair the oxygen carrying capacity of blood. Cigarette smoking is associated with coronary heart disease, stroke, ulcers, and an increased incidence of respiratory infections. Smoking is the major cause of lung cancer and is also associated with cancers of the larynx, esophagus, 7
bladder, kidney, pancreas, stomach, and uterine cervix. Smoking is also the major cause of chronic bronchitis and emphysema. Today, with universal awareness of the health risks, a billion adults worldwide make the choice to smoke. Nicotine is the major addictive substance in cigarette tobacco, given off by burning tobacco and carried into the respiratory tract on tar droplets and in the vapor phase. As a weak base, nicotine may exist in an ionized or a non- ionized form. The relative proportions of these two forms, determined by the pH of the smoke, affect where nicotine is most readily absorbed into the body. At the acidic pH of most cigarette smoke, absorption occurs predominantly in the lungs, but with the alkaline smoke produced by cigars and pipe tobacco, nicotine being predominantly non-ionized, is absorbed mainly in the mouth. Absorption into the blood stream is rapid, and concentrations of nicotine in the blood rise during smoking (Community Outreach Health Information System). Nicotine is metabolized, mainly in the liver and is a powerful psychoactive agent that has a variety of central and peripheral nervous system effects, as well as effects on the cardiovascular, endocrine, gastrointestinal, and skeletal motor systems. Self-administration of tobacco leads to physical nicotine dependence and psychological dependence on smoking behavior, with withdrawal symptoms associated with smoking cessation. The action of nicotine on the brain occurs rapidly after smoking, and this is believed to provide optimal reinforcement for nicotine dependence (S. Gebre Selassie, A. Gebre, Rapid assessment of drug abuse in Ethiopia, UNODC). The direct contribution of nicotine to tobacco-associated diseases is unclear, as it is inhaled along with many other substances in tobacco smoke. The role of nicotine is to maintain the addiction and other substances in tobacco smoke, particularly tar and some of the gaseous components such as carbon monoxide, are the main direct causes of diseases. 2.5 Toxicology Nicotine is a very toxic substance, and serious or fatal poisoning may occur as a result of the ingestion, inhalation or skin absorption of only small amounts. The lethal dosage of nicotine is 50 mg/kg for rats and 3 mg/kg for mice. 4060 mg can be a lethal dosage for adult person. This makes it an extremely deadly poison. It is more toxic than many other alkaloids such as cocaine, which has a lethal dose of 1000 mg (Bulletin on Narcotics 1996, Issue 1 - 004, pp 53 63). However the purpose of nicotine extraction is not for abuse.
8
2.6 Past Researches There are many methods used to extract nicotine from tobacco waste these include (a) supercritical extraction, (b) using an alkali (c) using distilled water. (a) Supercritical extraction When using supercritical extraction carbon dioxide pressurized to 7MPa is used as a solvent. Its advantages are that it is nontoxic and non-flammable and it has a low volatility. Carbon dioxide is fed through a valve then the solvent and feed are pressurized in a cell until pressure in the cell reaches 350atm and the temperature 50 degrees celcius.C0 2 is fed out at the top and nicotine is then collected. (b) Using an alkali Tobacco is mixed with an aqueous solution of soda; or is treated in any of the well-known methods with an alkali, such as hydrated lime, to free the nicotine from the other constituents of the tobacco waste (Vernellia R. Randall. 1999. History of Tobacco, Boston University Medical Center).The saturated alkaline mixture is then charged into a drier, which may be a horizontal cylinder provided with the usual dry blender agitator. Heat is applied to the drier by a steam jacket to the mixture of the saturated alkaline wastes which then vaporizes the nicotine freed from such wastes. After heating the nicotine-saturated, vapor is withdrawn from the drier through a dust filter to a condenser by means of a vacuum pump. (c) Using distilled water There is also a process of extracting nicotine using distilled water (Negarit Gazeta (Ethiopian journal), 1943 (1935 E.C.), Addis Ababa, Ethiopia, Article).Crushed tobacco is mixed with distilled water .Raw material: distilled water ratio is 1:3. The mixture is left for 24 hours in the dark after 24 hours a separator is used to separate nicotine from the tobacco waste. According to literature the test for nicotine is carried out by titration of the nicotine solution with sulphuric acid using bromothymol blue as an indicator and a colour change from pale blue to pale/ golden yellow serves as a positive test for the presence of nicotine (Nicotine Test Kit.htm). 2.7 The cold infusion process The use of high temperatures has complications in the dynamic industry climate. With increasing energy costs, people have resorted to use of low temperature systems that still make it possible to extract large amounts of nicotine. There are several reasons: 1) Low energy costs. 2) Simple techniques and apparatus are used. 9
3) No complex harmful chemicals used. 4) Low operating costs. 5) Cheap and readily available resources (water and tobacco waste). 6) High purity of nicotine achieved. 10
CHAPTER 3: RESEARCH METHODOLOGY
When tobacco is harvested there is accumulation of waste which can be made use of .Before using the waste in making manure, the waste can be used in the extraction of nicotine. This is a major advantage because the nicotine can be used as a raw material in the production of insecticides and pharmaceutical products.
3.1 Experiments The following experiments were carried out in order to extract nicotine and determine its content in tobacco waste using distilled water. Therefore, most of the apparatus, equipments and procedures were similar with the method of using an alkaline. Nevertheless, different modifications were made based on the objective, conditions and availability of equipments for this study. Below is a list of the experiments that were carried out. 1) Extraction of nicotine and testing for its presence. 2) Determination of nicotine concentration in the extract. 3) Separation of the nicotine in the solution from water.
3.1.1 Aim: To extract nicotine from tobacco waste using distilled water. 3.1.2 Objectives: 1) Extracting of the nicotine and testing for its presence. 2) Determining the nicotine concentration in the extract. 3) Separating nicotine from the extract using simple distillation. 3.1.3 Apparatus: 1) 2L and 200ml Beakers 2) Digital balance 3) Volumetric flask 4) Measuring cylinder 5) Filter paper 6) Dropper 7) Funnel 8) Burette 9) Pipette 11
10) Mixing cup 11) Safety goggles 12) Safety gloves 13) Simple distillation apparatus 3.1.4 Chemicals 1) 0,1M Hydrochloric acid 2) Distilled water 3) Bromothymol blue indicator solution 4) 0,1M sulphuric acid 3.2 Extraction of nicotine i) The tobacco waste was downsized by crushing. ii) 200g of tobacco waste was weighed and put into a beaker. iii) 600ml of distilled water was weighed and mixed with the tobacco waste. iv) The beaker was covered and left for about 24hours for solvent extraction. v) The mixture from the solvent extraction was filtered vi) The weight and volume of the residue and filtrate were measured. 3.3 Testing for the presence of nicotine i) 0,1M Sulphuric acid was prepared ii) A 3ml pipette was used to accurately measure exactly 3ml sample of nicotine solution to be tested, and dispensed into mixing cup. iii) Approximately 15ml to 20ml of distilled water, 6-8 drops of Bromothymol Blue pH indicator were added to mixing beaker and swirled to combine. iv) The second 3ml pipette was used to accurately measure exactly 3ml of 0.1M sulphuric acid. v) Titration with acid in small amounts using a burette to mixing cup and the solution was swirled until it started to turn green to greenish-yellow an indication of end point of the titration. vi) The amount of acid added was reduced to single drops and swirled after each drop until the solution turned from a pale greenish-yellow to all yellow. A yellow end point would be a positive test for the presence for nicotine in the solution. 12
3.4 Separation using simple distillation i) Nicotine solution was poured into a bottomed flask connected by means of a distillation adapter to a water-cooled condenser. ii) A thermometer was placed in the vertical arm of the distillation adapter by a special rubber connector at a height adjusted so that the top of the thermometer bulb would be 5 10 mm below the opening of the side-arm. iii) Whilst monitoring the temperature on the thermometer, the nicotine solution was heated using a burner iv) Distilled liquid (water) was collected in a clean, dry receiver. *An essential precaution to be taken into account was ensuring that the distilling assembly had an opening to the atmosphere to avoid developing a dangerously high pressure within the system during heat application. 3.5 Determining Nicotine Concentration Each 1ml of 0.1M Sulphuric Acid used during titration neutralized approximately 16.2mg of nicotine (Nicotine Test Kit.htm). To find the total amount of nicotine present in our sample, we multiply the amount of acid used by 16.226. Divide the total amount of nicotine from above by the sample size to determine the nicotine concentration in mg/ml. If starting with a 1ml sample of solution, divide total amount of nicotine by 1. If starting with a 2ml sample of solution, divide by 2. Calculations 11.5ml sample of nicotine solution was added to the mixing cup. During titration, 14.8ml of 0.1M acid was used to reach the yellow endpoint. Multiply 14.8 by 16.226, this gives us a total nicotine content of 240 (rounded) Dividing 240 by the 11.5ml starting sample size gives us a final test result of 20.87mg/ml (rounded)
13
Internet The information for the extraction of nicotine from tobacco waste using cold distilled water was obtained from for example (Wikipedia, the free on line Encyclopedia, URL:http://en.wikipedia.org/wiki/Nicotine). Text books The research team intends to visit a number of libraries including that of Harare Institute of Technology and University Of Zimbabwe as part of gathering pertinent information for the project where textbooks, magazines, journals and any relevant sources will be accessed. 3.6 Material and Energy balances Material and energy balances of the processes were conducted to help analyze the feasibility of the process of nicotine extraction.
14
CHAPTER 4: RESULTS AND ANALYSIS 4.1 Extraction of nicotine A thick dark brown colour was observed after adding distilled water to tobacco waste for 24 hrs. Addition of drops of Bromothymol blue the colour changed from dark brown to pale greenish-yellow and to bright yellow. 4.1.1 Conclusion for extraction of nicotine The thick dark brown colour observed confirmed that something had dissolved in the distilled water and as (Beyeh, M. and Abuhay, T. (2006.) tobacco production in Ethiopia and the constraints it has been facing.Addis Ababa: Ethiopian Institute of Agricultural Research), this was concluded that there was nicotine in the distilled water.
4.1.2 Testing for the presence of nicotine Yellow colour observed hence confirmed the presence of nicotine as (M.Gebre.Turkish- Tobacco. Rapid assessment of drug abuse in Ethopia, 2006.760, 20026-8071) suggested.
Figure 4.1 Experimental results showing presence of nicotine. 4.1.3 Conclusion for testing for the presence if nicotine The yellow colour confirmed the presence of nicotine as an alkaloid. Nicotine solution was successfully extracted from the tobacco waste materials used. Addition of bromothymol blue indicator to the nicotine solution reflected a light blue colour. 15
Titration of the solution with sulphuric acid resulted in a colour change from pale blue to pale/ golden yellow. The solvent used in extraction was recovered and recycled. 4.2 Analysis for testing the presence of nicotine 10ml of nicotine solution reacted with14.8ml of sulphuric acid using bromothymol blue indicator to reach the end point. Calculations 11.5ml sample of nicotine solution was added to the mixing cup. During titration, 14.8ml of 0.1M acid was used to reach the yellow endpoint. Multiply 14.8 by 16.226, this gives us a total nicotine content of 240 (rounded) Dividing 240 by the 11.5ml starting sample size gives us a final test result of 20.87mg/ml (rounded). 4.3 Conclusion This colour showed a positive test for nicotine presence.
16
CHAPTER 5.0 PROCESS DESIGN
Figure 5.1: Block flow diagram for the nicotine extraction process
This process is suitable for the extraction of nicotine from tobacco waste because the cost of the equipment is comparative low, simple and efficient in the extraction. The process also recycles the water, (condensed from the distillation column) for use in the mixing unit, therefore minimizing wastages .The waste is milled and reduced in size in the crushing unit to increase the surface area to dissolve the nicotine in the water. A filter press is to be used to ensure effective and quick filtration. A batch distiller is to be set since it is generally cheap than other types of distillation and also because nicotine and water have a very big difference in boiling points hence a batch distiller is the most ideal. 17
KEY: E-9----filter E-17----crusher E-18----conveyor belt E-19----mixer E-21----Distillation column E-24----compressor E-23----Condenser E-25----Storage tank P (1, 2, 4, 5, 9, 10, 32, 34, 35) ---pipelines 18
5.1 Mass balance at laboratory scale
595g water (recycle) 595g water
200g Tobacco 615g solution 600g water 20g
Figure 5.3: Mass balance diagrams
Mixer
Filter Distillation Column
Condenser 19
Overall Total Balance According to the data from the experiment of extraction of nicotine: I nput; Basis: 200g of tobacco waste (feed) 200g tobacco waste +600g distilled water =800g
Output: 185 g tobacco waste+595g water+20 g nicotine 800g 800g=800g 5.2 Energy Balance For The Extraction Process Extraction column (Open system) Input; Tobacco waste(s) + water (l) @ 25 o C Output; Water -nicotine solution (l) + residue (g) @ 25 o C H+K+P=Q-W S Q= 0 (since there is no change in temperature)
20
Energy balance on the distillation column The energy balances below illustrate quantity of heat energy which must be used to extract the nicotine. H (enthalpy) of water at 25 o C = 0.00KJ/mol H (enthalpy) of water at 100 o C = 2.54KJ/mol H = 2.54(for water) H=mCpT H= (0.02kg) (5.5940)(373
K -298
K) =8.39KJ. H+K+P=Q-W S W S Q=8.391KJ. H is positive hence our system used external energy .Higher enthalpy value corresponds to low pressures and this reduces costs and increases the performance of the equipment hence this shows that the project is economically feasible.
Heat required by the reboiler
Q = (mcpt +mL) water + (mcpt +mL) nicotine
=(0.595kg)(4.186KJ/Kg o C )(100 o C-25 o C)+(2257KJ/kg)(0.595kg)+( 0.02kg)(100 o C -25 o C) ( 5.594KJ/kg o C +(7.619KJ/Kg)(0.02kg) =1.538kJ
Heat rejected by condenser Q = mLwater +mL nicotine
H is positive hence our system used external energy .Higher enthalpy value corresponds to low pressures and this reduces costs and increases the performance of the equipment hence this shows that the project is economically feasible. Also our process requires exterior energy of 8.391 KJ to be operational. 21
CHAPTER 6.0 ECONOMIC ANALYSIS
Cost of tobacco wastes=US$0.10/kg (ZTA. www.irinews.com and Boka Tobacco Auction Floor) Cost of water=US$0.0260/ltr Selling price for nicotine= US$500/kg The raw material constitutes 7.4% of nicotine by mass Ratio nicotine: raw material 0.074kg : 1kg 6.1 Equipment 1. 1 Crusher= US$ 20 000 2 . 2 Distiller =US$ 44 000 3. 1Soaking vessel =US$ 7000 4. 1Separator =US$7 000 5. Piping =US$3 000 Demirbas,E.and Nas, M. (2009). Batch kinetic and equilibrium studies of , Newdeli: Gilberts.) 6.2 Scaling to industrial level Nicotine produced /tonne (0.02 x 1000kg )/0.2= 100kg nicotine/tonne raw material Water /tonne of raw material = 3000 liters Cost of water/tonne = 3000ltr x US$0.0260/liter = US$78/tonne (raw material) Cost of raw material/tonne = 1000kgxUS$0.1kg = US$100 22
Total cost /tonne=US$100/tonne
Table 6.1 Direct Costs Raw materials (tobacco waste, water) US$322000 Operating labor US$24000 Power US$44000 Equipment US$81000 Total US$471000
Table 6.2 Indirect Costs Construction expenses US$32000 Contingency US$9000 Taxes US$7000 Depreciation US$9000 Total US$57000
Table 6.3 Plants Overheads Warehouse US$30000 Security US$4000 Medical and Emergence services US$9000 Total US$43000
23
Table 6.4 General Expenses Administrative expenses US$11000 Distribution expenses US$50000 Research and Development US$42000 Total US$103000 6.3 Profit Analysis Income 1tonnes waste/day =100kgxUS$500=US$50000/day Income per month =US$50000x26 days a month=US$1300000 Totals Total Product Cost = US$674000 Profit=Sales-Total Product Cost Profit=US$1300000-US$674000=US$626000 6.4 Return on investment (ROI)
ROI = YEARLY PROFIT/ TOTAL INITIAL INVESTMENT Yearly profit= US$ 626 000 Total initial investment=US$1190000 ROI = US$626000/ US$1190000 X 100% = 52.6% Analysis The Return on Investment of 52.6% shows that the extraction project is worth investing in and hence equity investors will be interested in it.
The Payback Period=Initial investment Net Profit =US$1190 000/US$626 000 24
=1.9years Analysis At this proposed production rate all the capital will be returned approximately within two years and this shows that our investment is a better investment since it has a short payback period.
6.5 Break Even Analysis From calculations, BE=[F.C/SALES-V.C]*100 =US$57000/ (1300000-471000)*100 =6.9% Economically, this project is feasible and has got a reasonable profit hence it worth going for.
25
CHAPTER 7: CONCLUSION AND RECOMMENDATIONS
7.1 Conclusion The extraction of nicotine is a technically, economically and environmentally feasible process. The process on an industrial scale is profitable. The extraction process is safe for the workers at the plant. The most effective way of extracting nicotine from tobacco waste is solvent extraction using distilled water. 7.2 Recommendations
The best method for nicotine extraction with the best and competitive quality for the agricultural and pharmaceutical industries is the method of solvent extraction using distilled water. At present a bulk of the waste is utilized by the cigarette manufacturer themselves for manufacture of tobacco. All identified nicotine in waste materials should be utilized for manufacture of nicotine.
26
APPENDIX A1 Gantt Chart
Goals Actual progress
wk1 wk2 wk3 wk4wk5 wk6 wk7wk8 wk9 wk10wk11 wk12 wk13 wk14 wk15wk16 Project title selection Idea screening and brainstorming Document printing submission Project proposal and oral presentation Research and methodology Lab Experiments Results and Analysis Progress Report submission and presentation Mass and Energy Balance Economic Balance and Break Even Analysis Cash Flow Analysis Progress report 2 submission and presentation Model and Prototype construction Conclusion and recomentations Final submission and presentation 27
A 2 Cash Flow Forecast
Beginning Cash Balance $ Cash Inflows Beginning Cash Balance $ 100 000 Acct. rec. collections 183 000 Cash and cash equivalent sales 679 000 Total Cash Inflows $ 962 000 Cash Outflows Operations $ Raw materials 322 000 Operating Labour 24 000 Power 44 000 Equipments - purchase 81 000 Research & Development 42 000 Distributive Experiences 52 000 Total Operations $ 565 000 Finance & Administration Administrative Expenses $ 11 000 Warehouse 28 000 Security 4 000 Medical & Emergence Services 9 000 Taxes 7 000 Depreciation 9 000 Construction expences 30 000 contingency 9 000 Total Finance & Administration $ 109 000 Total Cash Outflows $ 674 000 Net Increase (Decrease) in cash $ 288 000 28
References 1) Hailu, W. and Kassa, W. (1979) Handbook of cultural practice for flue-cured Tobacco; london: Pan book. 2) Wikipedia, the free on line Encyclopedia, URL:http://en.wikipedia.org/wiki/Nicotine, accessed on March 12, 2014. 3) Gebre, S. and Selassie, A. (1996) Rapid assessment of drug abuse in Ethiopia; London: Chatto and Windus. 4) Brockley, M. (2004) Tobacco, TED Case Studies an Online Journal, . 5) Vernellia, R. and Randall, T. (1999). History of Tobacco, Boston: Boston University. 6) Compton, M. (1957) Sublime Tobacco, London: Chatto and Windus. 7) Negarit, G.(1943) Ethiopian journal, Addis Ababa: Ethiopian Articles. 8) Amharic, V. (2005)NationalTobacco Enterprise Sh.Co., Addis Ababa: Ethiopia. 9) Beyeh, M. and Abuhay, T. (2006.) tobacco production in Ethiopia and the constraints it has been facing.Addis Ababa: Ethiopian Institute of Agricultural Research 10) Christina, M. and Robert, F.(2001) tobacoo processing, Toronto: Hill Glenn. 11) Marsella, A. and Jiping, H. (1997) Tobacco, Culture, and Health among American Indians: A Historical Review, Journal Of Chemical Education, Washington: Academic Search Premier. 12) Demirbas, E. and Nas, M. (2009). Batch kinetic and equilibrium studies of , Newdeli: Gilberts. 13) Coilino, L. Wobick, K. (2001) The relative buffering capacities of saliva & moist snuff implications for nicotine absoption: Journal of Analytical Toxicology, Toronto: Hill Glenn. 14) Lockman, P. and Van der Schyf, C. (2005) Chrommic nicotine exposure alters blood brain barrier permeability and diminished brain uptake of methyllycaconitine: Journal of Neurochemistry, Washington: McGraHill. 15) Stepanov, I. Jensen, J. (2006) Tobacco- specific nitrosaminesin new tobacco products: Nicotine & Tobacco Research, London: London Press. 16) Lyon, H. (2007). International Agency for Research on cancer IAAC Monographs on the evaluations of caranogenic risk to humans,Washington: McGraHill. 17) Hawkins, B. etal (2002) Smoking and inschemic stock: a role for nicotine, Newdeli: Gilberts. 18) Herbet, R. (2001) What is new in nicotine & tobacco reservoir: Nicotine tobacco. Addis Ababa: Ethiopian Institute of Agricultural Research 29
20) Liu, X. and Wang, J. (2003) Molecularity Imprinted Solid- Phase Extraction Sorbent for removal of nicotine from Tobacco Smoke:Analytical Letters, Tokyo:Academic Search Premier. 21) Aksu, Z. Tezer, S. (2000).Equilibrium and kinetic modeling of biosorption of Remazol Black B by Rhizopus arrhizus in a batch system: effect of temperature, Beijing: Premier publishers.