EPA PROBIT ANALYSIS PROGRAM

USED FOR CALCULATING LC/EC VALUES

Version 1.5



LC50_24_Dhapnia_Fe

Proportion
Observed Responding Predicted
Number Number Proportion Adjusted for Proportion
Conc. Exposed Resp. Responding Controls Responding

50.0000 30 9 0.3000 0.3000 0.2857
100.0000 30 17 0.5667 0.5667 0.6067
150.0000 30 24 0.8000 0.8000 0.7764


Chi - Square for Heterogeneity (calculated) = 0.328
Chi - Square for Heterogeneity
(tabular value at 0.05 level) = 3.841

Mu = 1.902617
Sigma = 0.359834

Parameter Estimate Std. Err. 95% Confidence Limits
---------------------------------------------------------------------
Intercept -0.287479 1.421857 ( -3.074318, 2.499361)
Slope 2.779056 0.724478 ( 1.359080, 4.199032)

Theoretical Spontaneous Response Rate = 0.0000


LC50_24_Dhapnia_Fe

Estimated LC/EC Values and Confidence Limits

Exposure 95% Confidence Limits
Point Conc. Lower Upper

LC/EC 1.00 11.629 1.341 23.786
LC/EC 5.00 20.451 4.217 34.878
LC/EC 10.00 27.635 7.740 42.918
LC/EC 15.00 33.860 11.631 49.498
LC/EC 50.00 79.913 58.176 101.122
LC/EC 85.00 188.603 137.609 436.855
LC/EC 90.00 231.090 159.418 653.517
LC/EC 95.00 312.254 196.871 1195.211
LC/EC 99.00 549.161 289.525 3746.143

LC50_24_Dhapnia_Fe

PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE

Probit
10+
-
-
-
-
9+
-
-
-
-
8+
-
-
- .
-
7+ .
- ..
- ..
- ...
- ....
6+ ....
- .o..
- ...
- ....
- ...o
5+ ...
- ....
- ....
- ..o
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