Lactobacillus MRS Agar

M641
Lactobacillus MRS Agar is recommended for cultivation of all Lactobacillus species.
Composition**
Ingredients Gms /
Litre Proteose peptone 10.00
0 Beef extract 10.00
0 Yeast extract .00
0 !extrose "0.00
0 Pol#sorbate $0 1.00
0 Ammonium citrate ".00
0 Sodium acetate .00
0 Magnesium sulp%ate 0.10
0 Manganese sulp%ate 0.0
0 !ipotassium p%osp%ate ".00
0 Agar 1".00
0 &inal p' ( at ")*+ ,.-0
."
..&ormula ad/usted0 standardi1ed to suit performance parameters
Directions
Suspend ,2.1 grams in 1000 ml distilled 3ater. 'eat to boiling to dissolve t%e medium
completel#. Sterili1e b# autoclaving at 1 lbs pressure (1"1)*+ for 1 minutes. Mix 3ell and pour
into sterile Petri plates.
Principle And Interpretation
Lactobacilli MRS medium is based on t%e formulation of deMan0 Rogosa and S%arpe (1+ 3it% slig%t
modi4cation. 5t supports luxuriant gro3t% of all Lactobacilli from oral cavit# (1+0 dair# products ("+0
foods (6+0 faeces (7+ and ot%er sources (+.
Proteose peptone and beef extract suppl# nitrogenous and carbonaceous compounds. Yeast
extract provides vitamin B complex and dextrose is t%e fermentable carbo%#drate and energ#
source. Pol#sorbate $0 supplies fatt# acids re8uired for t%e metabolism of Lactobacilli. Sodium
acetate and ammonium citrate in%ibit Streptococci0 moulds and man# ot%er microorganisms.
Magnesium sulp%ate and manganese sulp%ate provide essential ions for multiplication of
lactobacilli. P%osp%ates provide good bu9ering action in t%e media.
Lactobacilli are microaerop%illic and generall# re8uire la#er plates for aerobic cultivation on solid
media. :%en t%e medium is set0 anot%er la#er of un;inoculated MRS Agar is poured over t%e
surface to produce a la#er plate (+. Lactobacilli isolated on MRS Agar s%ould be furt%er con4rmed
bioc%emicall#.
ualit!
Control
Appearance
*ream to lig%t #ello3 %omogeneous free <o3ing po3der
Gelling
&irm0 comparable 3it% 1."= Agar gel.
Colour and Clarit! o" prepared medium
Medium to dar> amber coloured0 clear to slig%tl# opalescent gel forms in Petri plates
Reaction
Reaction of ,.21= 3?v a8ueous solution at ")*. p' @ ,.-0."
p#
,.60;,.20
Cultural Response
*ultural c%aracteristics observed after an incubation at 6;62)* for 1$;"7 %ours or longer.(3it% =
*A"+
Please re"er disclaimer $%erlea"&
#iMedia Laboratories 'ec(nical Data
Cultural Response
$rganism Inoculu
m
)C*+,
Gro-t( Reco%er!
Lactobacillus casei
ATCC 9595
0;100 luxuriant BC0=
Lactobacillus
fermentum ATCC
9338
0;100 luxuriant BC0=
Lactobacillus
leichmannii ATCC
7830
0;100 luxuriant BC0=
Lactobacillus
plantarum ATCC
8014
0;100 luxuriant BC0=
Storage and S(el" Li"e
Store belo3 60)* in tig%tl# closed container and t%e prepared medium at " ; $)*. Dse before expir#
date on t%e label.
Re"erence
1.deMan E.0 Rogosa M. and S%arpe M.0 1F,00 E. Appl. Bacteriol.0 "6@160.
".Mars%all R.G. (Hd.+0 1FF"0 Standard Met%ods for t%e Hxamination of !air# Products0 1,t% ed.0
AP'A0 :as%ington0!.*. 6.!o3nes &. P. and 5to I.0 (Hds.+0 "0010 *ompendium of Met%ods
&or t%e Microbiological Hxamination of &oods0 7t% Hd.0 AP'A0
:as%ington0 !.*. 7.Sabine and Jasele>os0 1F,0 Kature0 "0,@F,0.
.Mac&addin E.01F$0 Media for 5solation;*ultivation;5denti4cation;Maintenance of Medical Bacteria0
Jol.10 :illiams and :il>ins0 Baltimore.
Revision @ 1 ? "011
!isclaimer @
Dser must ensure suitabilit# of t%e product(s+ in t%eir application prior to use. Products conform solel# to t%e
information contained in t%is and ot%er related 'iMediaL publications. G%e information contained in t%is publication is
based on our researc% and development 3or> and is to t%e best of our >no3ledge true and accurate. 'iMediaL
Laboratories Pvt Ltd reserves t%e rig%t to ma>e c%anges to specifications and information related to t%e products at
an# time. Products are not intended for %uman or animal or t%erapeutic use but for laborator#0diagnostic0 researc% or
furt%er manufacturing use onl#0 unless ot%er3ise specified. Statements contained %erein s%ould not be considered as a
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Prinsip dan interpretasi
MRS la>tobasili menenga% didasar>an pada perumusan deMan0 Rogosa dan
S%arpe (1+ dengan sedi>it modi4>asi. Mendu>ung pertumbu%an lebat semua
la>tobasili dari rongga mulut (1+0 produ> susu ("+0 ma>anan (6+0 tin/a (7+ dan
sumber;sumber lain (+.
Proteose peptone dan daging sapi e>stra> paso>an sen#a3a nitrogen dan
mengimbu%>an. H>stra> ragi men#edia>an vitamin B >omple>s dan de>strosa
adala% Beragi sumber >arbo%idrat dan energi. Pol#sorbate $0 paso>an asam
lema> #ang dibutu%>an untu> metabolisme Lactobacillus. Katrium asetat dan
amonium sitrat meng%ambat strepto>o>us0 ceta>an0 dan ban#a>
mi>roorganisme lainn#a. Magnesium sulfat dan mangan sulfat men#edia>an
esensial ion per>alian dari Lactobacillus. &osfat memberi>an tinda>an bu9er
#ang bai> di media.
Lactobacillus microaerop%illic dan umumn#a memerlu>an lapisan piring untu>
aerobi> budida#a pada media padat. Ieti>a media diatur0 lapisan lain un
terlepas MRS Agar dituang>an di atas surfa