RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA BANGALORE
ANNEXURE-II
PROFORMA FOR REGISTRATION OF SUBJECT FOR
DISSERTATION
1. Name ! "#e $a%&'&a"e a%& a&&(e))
*'% +,$- ,e""e().
D(. RASHMI SINHA
Dept of Biochemistry,
M.R Medical College,
Gulbarga 58515
/. Name ! "#e I%)"'"0"'% !.".# $ociety%s
M.R Medical college,
Gulbarga&58515
1. C0()e ! S"0&2 a%& )0+3e$"
M.D. 'Biochemistry(
4. Da"e ! A&m'))'% ! C0()e
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5. T'",e ! "#e "6'$
7STUDY OF SERUM 8 URINE
PROTEIN THIOLS IN ESSENTIAL
HYPERTENSION9
:. B('e! Re)0me ! "#e I%"e%&e& ;(-
:.1. Nee& !( "#e S"0&2<
#sse,tial hyperte,sio, is commo, i, de-eloped
societies . is a ma/or ris0 factor for
cardio-ascular disease. 1t is li0ely to be the o,e of
the co,se2ue,ces of a, i,teractio, bet3ee,
e,-iro,me,tal . ge,etic factors.
1
$e-eral pre-ious studies ha-e i,dicated that
esse,tial hyperte,sio, is a state of i,creased
o4idati-e stress 3ith i,creased reacti-e o4yge,
species 'R5$( . imbala,ce of R5$ . the
a,tio4ida,t defe,se mecha,ism.
),6

7he thiol group o, protei,s has a, importa,t
role i, a,tio4ida,t status of the body. 7his group
of serum protei,s especially albumi, has bee,
suggested to play a, importa,t role as a,tio4ida,t
i, plasma . e4tra-ascular spaces
*
. $e-eral
i,-estigators suggested the e,ha,ced thiol group%s
o4idatio, i,-ol-ed i, the pathoge,esis of
re,o-ascular hyperte,sio,.
5,8,9
!o3e-er, the
i,formatio, regardi,g o4idati-e protei, damage
i, patie,ts 3ith esse,tial hyperte,sio, ha-e ,ot
bee, studied e4clusi-ely . there is paucity of
i,formatio, i, the literature i, this regard.
:s it is 3ell 0,o3, fact that about 8; to *; of
u,co,trolled hyperte,sio, is associated 3ith
protei,uria . albumi, is a ma/or protei, e4creted
i, uri,e of patie,t.
8,+,1
7he curre,t study is
desig,ed to establish the relatio,ship bet3ee,
serum albumi, . protei, thiols 3ith protei,
bou,d thiols i, the uri,e of esse,tial hyperte,si-e
patie,ts.
:./. Re='e; ! L'"e(a"0(e< 1, huma,s, hyperte,sio, is also co,sidered as a
state of o4idati-e stress that ca, co,tribute to the
de-elopme,t of atherosclerosis.
11
<re-ious studies promoted the -ie3 that esse,tial
hyperte,sio, is a state of i,creased o4idati-e
stress a,d the data o, o4idati-e protei,
modificatio, is lac0i,g.
)
:-ailable e-ide,ces supports a role of protei,
thiol groups i, this process as they represe,t a
3ell 0,o3, target reacti-e species
1),16
1,dra/it $i,ha et al
1*
fou,d that mar0ers of
o4idati-e stress correlated sig,ifica,tly 3ith
protei,uria i, ,ephrotic sy,drome patie,ts, 3hich
is i, accorda,ce to the pre-ious studies.
:mo,g hyperte,si-e sub/ects, patie,ts 3ith
microalbumi,uria ha-e i,creased ris0 of
cardio-ascular complicatio,s.
15,18

:.1. O+3e$"'=e ! "#e )"0&2<
7he prese,t study aims to assess the cha,ges i,
the serum . uri,e protei, thiol le-el i, esse,tial
hyperte,si-e patie,ts.
>. Ma"e('a, a%& me"#&)
>.1 S0($e ! Da"a<
7he study 3ould be u,derta0e, at Basa-esh3ar
7eachi,g a,d Ge,eral !ospital attached to
M.R.Medical College, Gulbarga.
<rese,t study co,sists of 5 cli,ically diag,osed
cases of esse,tial hyperte,sio, i, the departme,t
of Medici,e duri,g the period of Dec )+ to
=u,e )11.
Co,trols 3ould co,sist of ,o,&hyperte,si-e
perso,s 3ho 3ill be carefully selected .
e4ami,ed i, detail 3ith age . se4 matched.
>./ Me"#&) ! $,,e$"'% &a"a<
'i,cludi,g sampli,g procedure if
a,y(
>,der aseptic co,ditio,s, blood samples 3ill be
dra3, i,to plai, -acutai,ers from the a,tecubital
-ei, .7he collected blood 3ill be allo3ed to clot
. the, ce,trifuge at )rpm for 15 mi,ute for
clear separatio, of serum.
?rom the same cases . co,trols, )*hr uri,e
samples 3ill be collected i, bottles co,tai,i,g
tolue,e . 3ill be the, assayed for uri,ary
protei,s . protei, thiols.
Me"#&)& $erum . >ri,e protei, thiols 3ill be
measured by $pectrophotometric method usi,g
5,5@dithio&bis)&,itrobe,Aoicacid'D7BB(,$erum
creati,i,e, serum albumi,, uri,ary total protei,s
a,d creati,i,e le-els 3ill be estimated by
spectrophotometric methods usi,g a,
$emiautoa,alyser '#RB:&C!#M 9(.
I%$,0)'% C('"e('a<
Cases cli,ically diag,osed as esse,tial
hyperte,sio, i, departme,t of Medici,e at
Basa-esh3ar 7eachi,g . Ge,eral !ospital,
attached to M.R.Medical College, Gulbarga.
E?$,0)'% C('"e('a<
Cases of esse,tial hyperte,sio, associated 3ith
Diabetes, obesity, $mo0i,g a,d other types of
!yperte,sio,.
>.1 DDe) "#e )"0&2 (e@0'(e& a%2
'%=e)"'Aa"'% 8 '%"e(=e%"'% "
+e $%&0$"e& % 6a"'e%") (
"#e( #0ma%) ( a%'ma,)B I! ),
6,ea)e &e)$('+e +('e!,2.
7he prese,t study re2uires i,-estigatio, to
co,duct o, huma,s as per ethical guideli,es.
Colu,teer 3ritte, i,formed co,se,t 3ill be
obtai,ed from all the patie,ts . co,trols of the
study.
>.4 Ha) e"#'$a, $,ea(a%$e +ee%
+"a'%e& !(m 20( '%)"'"0"'%
'% $a)e ! >.1
Des, #thical cleara,ce has bee, obtai,ed from the
ethical committee of the i,stitutio, for the study.
C. L')" ! Re!e(e%$e)
1.!arriso,%s <ri,ciples of 1,ter,al Medici,eE 19
th
editio, pg.,o.& 155*.
).$imicDC,Mimic&50a=,<l/esa&#rcego-ac,$a-ic&ado/e-ic,5pacic M,MaticD,1-a,o-ic B a,d
$imic. =our,al of huma, hyperte,sio,.8 Dec )5FdoiE1.168Gs/./hh.11+*5.
6.$toc0er."ea,ey.Role of o4idati-e modificatio,s i, :therosclerosis.<hysiolRe- )6F
8*E1681& 1*98.
*.!alli3ellBF:,tio4ida,ts.huma, diseaseF a ge,eral i,troductio,,Butr Re-1++9,55,5**&55).

5.1,agi R, Miyata 7 .54idati-e protei, damage 3ith carbohydrates . lipids i, uremiaE%Carbo,yl
stress%Blood <urif 1+++F 19E+5&+8.
8.!immelfarb =,McMo,agle #,McMe,ami, # .<lasma protei, thiol o4idatio, . carbo,yl
formatio, i, chro,ic re,al failure. "id,ey 1,t )F 58E)591&)598.
9. Mimic 50a = ,$imic 7, <l/esa M, $tupar B, 7ur0o-ic $. 54idati-e modificatio,s of plasma
protei,s i, differe,t stages of chro,ic re,al failure.?acta >,i-arsitalis )1F8E1&5.
8.G.Crippa.Microalbumi,uria i, esse,tial hyperte,sio,E =our,alof !uma, !yperte,sio, )) F
18 E$9* $99.

+.Rosa,7orres 7a,iaF<alati,i,<aolo.Cli,ical -alue of microalbumi,aria i, hyperte,tio,.=our,al
of huma, !yperte,tio,, =u,e&)&Colume18&1ssue8 p 8*5&85*.

1.Barry M Bre,,er,7he "id,ey,8
th
editio,, page ,o. 1*96&1*9*.

11. Romero =C, Rec0elhoff =?. Role of a,giote,si, a,d o4idati-e stress i, esse,tial
hyperte,sio,. !yperte,sio,. 1+++F 6*E+*6+*+.

1).Hohose DH, De,u =M, $a,toro B a,d Di4o, =1E Role of aspartic acid&181 a,d $eri,e
&))) i, i,termediate formatio, a,d hydrolysis of mammalia, protei,&tyrosi,e&phosphatase
<7<1.Biochemistry68E*588&*595, 1++9.
16.Iha,g IDa,d Di4o, =#E:cti-e site labeli,g of the Dersi,ia protei, tyrosi,e phosphatase the
determi,atio, of the p"a of the acti-e site cystei,e a,d the fu,ctio, of the co,ser-ed histidi,e
*)FBiochemistry 6)E+6*&+6*5.
1*.$i,ha1,dra/it,Ghosh$a,dip,<rase,/it Dey,=ose =acob a,dDibya/yoti Ba,er/ee. Reductio, of
uri,ary thiols i, ,ephrotic sy,drome& a possible effect of free iro,E Cli,ica chimica :ctaF
Colume 655, issues 1 )E May )5.
15.Redo, = et al.?actors related to the prese,ce of microalbumi,uria i, esse,tial
hyperte,tio,.:m = !yperte,s 1++*F 81&89.
18. <edri,elli < et al.Microalbumi,uria a,d e,dothelial dysfu,ctio, i, esse,tial
hyperte,tio,.Ha,cet 1++*F6**E1*&18.
D. S'A%a"0(e ! "#e Ca%&'&a"e
1E. Rema(-) ! "#e G0'&e
#sse,tial hyperte,sio, is a state of o4idati-e stress.
Because of such o4idati-e e,-iro,me,t, it could
lead to decrease i, serum a,d uri,e protei,
thiols.7he prese,t study is ta0e, up to establish the
cha,ges duri,g esse,tial hyperte,sio,.
Recomme,ded . ?or3arded for 0i,d accepta,ce.
11.
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