An investigatory project submitted as an entry to the local

Science Fair S.y 2013-2014.

(Team Category)

Father Saturnino Urios University


PROPONENTS:
Jennicah Rhaiza p. Guzon
Daphne Jane C. Jumonong
Ivana A. Manliguez


Advisers:
Mrs. Maricar A. Haloot
Mrs. Flordeliz A. Villaluz





TABLE OF CONTENTS

Title Page ---------------------------------------------------- i

Abstract --------------------------------------------------- ii

CHAPTER I- Introduction ---------------------------------------------------- 1
A. Background of the Study
B. Statement of the Problem
C. Significance of the Study
D. Scope and Limitations

CHAPTER II- Review of Related Literature -------------------------------- 2

CHAPTER III Methodology ---------------------------------------------------- 3

CHAPTER IV Result and Discussions ----------------------------------------- 4

CHAPTER V Conclusion and Recommendation ---------------------------- 5

Bibliography ------------------------------------------------------------------------- 6













ABSTRACT


Synthetic pesticides are known to be dangerous to ones health because of its
hazardous chemical content. To contribute in maintaining the good health of
vegetable plants and preserving their nutrients, the researchers made use of Radish
extract as pesticide. They believe that Radish extract pesticide provides a safe yet
equally profitable effect and poses no threat to the health of the people.
In this study, the effectiveness of Radish tubers extract as pesticide was
tested. The researchers used one kilogram of radish tubers then pounded it with
mortar and pestle. They got the extract with the use of cheese cloth and prepared
four set- ups. In first set up, radish extract is added with water in a ration of 1:2. In
second set up with 1:1 ratio, in third set up with 2:1 ratio and the fourth set up with
pure extract. All extracts from each set up were used and sprayed in same kind of
pests such as: ants, worms and aphids.
Based on the data gathered it revealed that radish extract can be used as
pesticide and is effective in killing certain pests. It was also found out that pure
radish extract is more effective rather than when water was added to it. It is one
hundred effective in worms and ants and fifty percent effective in aphids.










CHAPTER I
Introduction
A. Background of the Study
Radish is an annual herb widely grown for its edible fleshy, hot tasting roots maybe red,
white or purple. Every part of the root or the plant can be used in different ways. The leaves and
the roots are eaten raw in salads. Likewise, it can be cooked or mix with meat to satisfactory
attain its delicious taste. Aside from food, it is dried for juice extraction.
Radishes were probably native to China. It was developed from a live plant that grows in
the cooler region of Asia. It spread to the Mediterranean region before the Greek era and was
introduced into the new world early in the 16th century. Radishes grow in Asia, Europe and
America. It was the favorite of the ancient Egyptians, Greek and Chinese
There is a little food value in the radish. It is use chiefly as a relish or to decorate salads.
Radishes are the easiest of all garden vegetables to grow, but they should be given in a good rich
soil. The first seed must be sown as soon as the ground is workable and not too cold for the seed
to germinate

B. Statement of the Problem
What is the effect of radish extract as pesticide on certain pests?

C. Significance of the Study
In choosing this project, we wanted to contribute knowledge in maintaining the good health
of vegetable plants by using a pesticide out of radish extract. It maybe helpful in livelihood by
producing good crops. The harvest can be sold to market as an additional income. It can also
minimize the number of planters who used chemical which are found distractive to the
environment.

D. Scope and Limitation

This study focused on the utilization of the extract from radish as pesticide against
different kind of pests like worms, ants and aphids. Experimentation was done in the school
laboratory and at home and was applied in affected green bell pepper plant.
1.

CHAPTER II
Review of Related Literature



Radish, Raphanus sativus, belongs to the cabbage family, rassicaeae. It is grown for its
fleshy root, which varies in sizes from the few grams of popular red American and European
varieties to the one or more kilograms of the Japanese white or draikon.
There are two kinds of radish, one is planted for its crops and the other for its leaves and roots.
Some varieties include Japanese radish, a big and long late harvest radish; and the China radish
which is fine smooth and white. The latter is productive and has a moderate length of time for
harvest. The icicle radish is white, early harvested and its long with small roots. Planters can
harvest the plant in 60 days and other can harvest within 100 days from planting.



















2.

CHAPTER III
Methodology

A. Materials:

The researchers need the following materials: mortar and pestle for pounding, empty
bottle sprayer, clean cheese cloth, knife, bowl, water and one kilogram radish tubers. They need
to get pure radish extract, 1:2 proportion of extract to water, 1:1 proportion and 2:1 proportion.


B. Procedure:

First thing to be done is to prepare all the materials needed. Collect one kilogram
of radish tubers and wash them to remove dirt. Slice into small pieces then pound it by
using a mortar and pestle. Put in a clean cheese cloth and squeeze it to get the extract.
Add enough water to the extract in a ration of 1:1, 1:2, 2:1 respectively. Place
each mixture in an empty sprayer and apply it into aphids, worms and ants in plants. Try
several trials to determine the more effective proportion. Record the observation








3.

CHAPTER IV
Result and Discussion

A. Result
Table 1
Observation on the insecticidal effect of radish extract in certain pests
Trial Proportion Volume Length of Number of Insects
Extraction ML Application Worms Ants Aphids

Mean
1 pure 250 10 10 10 all found in one fruit
2 2:1 250 10 10 10 all found in one fruit 10
3 1:1 250 10 10 10 all found in one fruit 10
4 1:2 250 10 10 10 all found in one fruit 10

Table 2
Insecticidal effect of radish extract
Trial Number of Insects Killed Percentage

Worms Ants Aphids Worm Ants Aphids
1 10 10 half 100 100 50
2 1 2 none 10 20 0
3 0 0 none 0 0 0
4 0 0 none 0 0 0
B. Discussion
It reveals that pure radish extract is more effective rather that when water was added to it. It
is 100% effective in worms and ants, and 50% effective in aphids. This shows that the radish
extract is very effective in killing certain pests.
After several experimentations, we found out that pure 100% radish extract when applied to
aphid, worms, and ants is an effective pesticide. This pesticide can minimize the number of
people who used chemical found and to affect our health and environment.

4.


CHAPTER V
Conclusion and Recommendation



A. Conclusion

Based on the data gathered. We therefore conclude the following:
1. The radish extract can be used as pesticide.
2. Pure radish extract is effective in killing certain pests.


B. Recommendation:

We recommended a more experimentations about radish extract must be done to improve the
quality of the product.















5.



BIBLIOGRAPHY

De Padua, Ludivina S.; Lugod, Gregorio C.; Pancho, Juan V.; Handbook of Philippine Medical
Plants Volume 1; College of Science and Humanities University of Philippines Los Baos
College, Laguna; Documentation and information Section Office of the Director of Research,
University of the Philippine, Los Baos; 1977

Maramba, Nelia P.; Saludez, Jerry D.; Guide Book on the Proper Use of Medicinal Plants;
Bicutan, Taguig Metro Manila; National Science and Technology Authority; 1982
Reyes, Angelica G.; Jovita G.; Santiago, Florenda I.; Philippine Plants; Philippine Christian















6.













FLOWCHART
























Collect and wash Radish tubers
Slice radish tubers into small pieces and
put in mortar and pestle then pound it
Put the extract into an empty spray
bottle
Put pounded radish into clean cheese
cloth. Squeeze it to get the juice
Make mixtures of extract and water and
put it into separate empty spray bottles.
Spray each mixture into pests of the
same species. Observe.

ABSTRACT

Making use of radish extract as an effective alternative method of getting rid of insects and
pests like worms, ants, and aphids was studied in this research project. The purpose of this
experiment is to prove that radish extract can be used as an insecticide.

The researchers first gathered all the materials needed to complete the experimentation.
First washed, sliced, and pounded the radish tubers using the mortar and pestle. Then the
researchers extracted it by using a clean strainer, strained and acquired the juice of the radish.
After completing the three setups, the researchers sprayed it on the insects and observed what
happened. They tried several trials to determine the more effective proportion and gathered all
the recorded observations.

From the experiment conducted, the researchers observed that pure radish extract is more
effective than the one which was added with water. This shows that the radish extract is
effective in killing certain pests. Therefore, the researchers conclude that the pure radish extract
can be used as pesticide and is more effective than the mixture of the combined water and
extract.

STATEMENT OF THE PROBLEM

This research study helps everyone to know whether the radish extract is really an effective
alternative pesticide; thus, not only helping people to save and apply practicality, but also
reduce the number of people using chemicals as pesticide which are distractive to nature. The
study entitled, Radish Extract as a Pesticide is guided by the following questions:

1. What is the effect of radish extract as pesticide on certain pests?
2. Can radish extract be effectively used as a pesticide? [continues]










What chemical substances is in a radish
that can be used as pesticide?
In: Chemistry [Edit categories]
Answer:
The only chemical thumbnail of radish I could find in popular scientific references, is this
one:
"The raw flesh has a crisp texture and a pungent, peppery flavor, caused
byglucosinolates and the enzyme myrosinase which combine when chewed to form allyl
isothiocyanates, also present in mustard, horseradish, and wasabi."


Of which the glucosinolates have possible pesticidal properties according to this citation:
"Besides being bitter tasted in cooking, plants producing large amounts
ofglucosinolates are also desirable, because substances derived from these can serve
asnatural pesticides and are under investigation in the prevention of cancer (with
sulforaphane in broccoli being the best known example).