A Novel Multiple Hypothesis Based

Particle Tracking Method for Clathrin

Mediated Endocytosis Analysis Using
Fluorescence Microscopy

In order to quantitatively analyze biological
cellular and subcellular processes
involved in theseprocesses. Manual tracking can be performed by the biologists, but the
workload is very heavy. In this paper, we present an automatic particle tracking method for
analyzing an essential subcellular
framework of the tracking method is an extension of the classical multiple hypothesis
tracking (MHT), and it is designed to manage trajectories, solve data association problems,
and handle pseudo-splitting/merging events. In the extended MHT framework, particle
tracking becomes evaluating two types of hypotheses. The first one is the trajectory
hypothesis, to test whether a recovered trajectory is correct, and the second
observation-related hypothesis, to test whether an observation from an
real particle. Here, an observation refers to a detected particle and its feature vector. To
detect the particles in 2D fluorescence
microscopy, the images are segmented into regions, and the features of the particles are
obtained by fitting Gaussian mixture models into each of the
are developed according to the properties of the par
demonstrated on synthetic data under different scenarios and applied to real data.
A Novel Multiple Hypothesis Based
Particle Tracking Method for Clathrin
Mediated Endocytosis Analysis Using
Fluorescence Microscopy
In order to quantitatively analyze biological images and study underlying mechanisms of the
processes, it is often required to track a large number of particles
. Manual tracking can be performed by the biologists, but the
workload is very heavy. In this paper, we present an automatic particle tracking method for
ng an essential subcellular process, namely clathrin mediated endocytosis. The
framework of the tracking method is an extension of the classical multiple hypothesis
tracking (MHT), and it is designed to manage trajectories, solve data association problems,
splitting/merging events. In the extended MHT framework, particle
tracking becomes evaluating two types of hypotheses. The first one is the trajectory
hypothesis, to test whether a recovered trajectory is correct, and the second
related hypothesis, to test whether an observation from an
real particle. Here, an observation refers to a detected particle and its feature vector. To
detect the particles in 2D fluorescence images taken using total internal reflection
are segmented into regions, and the features of the particles are
obtained by fitting Gaussian mixture models into each of the image regions. Specific models
are developed according to the properties of the particles. The proposed tracking method is
demonstrated on synthetic data under different scenarios and applied to real data.
A Novel Multiple Hypothesis Based
Particle Tracking Method for Clathrin
Mediated Endocytosis Analysis Using

and study underlying mechanisms of the
, it is often required to track a large number of particles
. Manual tracking can be performed by the biologists, but the
workload is very heavy. In this paper, we present an automatic particle tracking method for
, namely clathrin mediated endocytosis. The
framework of the tracking method is an extension of the classical multiple hypothesis
tracking (MHT), and it is designed to manage trajectories, solve data association problems,
splitting/merging events. In the extended MHT framework, particle
tracking becomes evaluating two types of hypotheses. The first one is the trajectory-related
hypothesis, to test whether a recovered trajectory is correct, and the second one is the
related hypothesis, to test whether an observation from an image belongs to a
real particle. Here, an observation refers to a detected particle and its feature vector. To
al internal reflection
are segmented into regions, and the features of the particles are
regions. Specific models
ticles. The proposed tracking method is
demonstrated on synthetic data under different scenarios and applied to real data.