Bio-Rad Molecular Imager® VersaDoc™ MP 5000

Sole Source Specifications

The Molecular Imager VersaDoc MP 5000 System is manufactured solely by Bio-Rad Laboratories, Inc.
It is a unique multi-imager for visualizing non-isotopic samples such as fluorescent, chromogenic and
chemiluminescent labeled samples. This versatile system is easily customized to accommodate today’s
applications and tommorow’s research. Unique LED epi-illumination and UV transillumination sources
are both supported for optimal imaging and uniformity. The flexibility of the VersaDoc MP 5000 is the
cornerstone of this system. Red, green, and blue LED-based illumination enables versatility for multi-
fluorescence and multiplex imaging. Broadband UV excitation allows for larger Stokes shifts and better
discrimination between excitation and emission wavelengths. The Windows and Mac compatible
software included with the VersaDoc MP 5000 allows for complete acquisition control and analysis of
gels, blots and films.

The features below and their benefits together combine to make the VersaDoc MP 5000 System a unique
offering to researchers.

1. Only system with patented true dynamic flat fielding using external reference plate. This eliminates
imaging non-uniformities caused by variations in the optical path due to zoom setting, illumination,
and/or lens.
2. Flat fielding correction results in a CV of ≤5% over the entire imaging area.
3. Only system with filters positioned between camera lens and camera, resulting in no mis-registration
between multi-channel images.
4. Only system that uses F-mount lenses, which are much higher optical quality than c-mount lenses.
5. Modular design allows upgrades to higher end cameras or future cameras, eliminating future
constraints.
6. Custom filter positions allow flexibility for future techniques.
7. True 16-bit system with 24 x 24 µm pixels (in order to be a true 16-bit system, pixels must be at least
16 x 16 µm).

Feature Benefit

♦ Cooled CCD Camera (Sole Source Specifications for VersaDoc MP 5000)

- Large Pixel Size (24 x 24 micron)
- Large well capacity (330,000 e- per pixel)
- Neglible read noise (8 e- per pixel)
- Blue Sensitive CCD
- Cooled to -25°C
- ~90% Quantum Efficiency
- Thermoelectric Cooling System
-True 16-bit Data Acquisition
-512 x 512 Pixel Array
- Improved general sensitivity
- Unsurpassed dynamic range among CCD imagers
- Further enhances signal-to-noise ratio
- Improved chemi sensitivity
- Reduced background and greater signal-to-
noise ratio with longer integration times for chemi
applications
- Highly photon sensitive for rapid detection of low
luminescent signals
- Very stable temperature and rapid adjustment
- Highly quantitative, 4.8 orders of linear
dynamic range
- High quality images

♦ Red, Green, and Blue LED (Sole Source Specifications for VersaDoc MP 5000)
Epi-Illumination - Versatile multiplex imaging and analysis of gels, blots,
and arrays
- Visible excitation reduces damage to precious
samples
- Highly uniform, CV of ≤5%, for quantitative illumination

Page 1 of 3

♦ UV and White Light Trans-Illumination
♦ Broad Bandwidth UV (290-365nm)
Illumination
♦ White Light Excitation
♦ Customized Image Acquisition
- Dark Current Subtraction
- Dead Pixel Correction
- Hot Pixel Correction
♦ Lenses
- 28-80 mm Zoom Lens
- Aspheric Optics
- 50mm, f1.4 Fixed Lens
- Optional 105 mm Lens
- Supports Custom Lenses
♦ Large Imaging Area
♦ Visible, UV and White Light
Illumination
♦ Filters
- Eight (8) Position Filter Wheel
- Custom Filters
♦ Sample tray, highly absorbent
Feature
♦ Multi-Channel Imaging
Page 2 of 3
- Class 2 laser excitation efficiency, without the cost of a
laser scanner
- Fully integrated into hardware and software design,
promoting ease-of-use
(Sole Source Specifications for VersaDoc MP 5000)
- Analysis of blots, film, and opaque samples
with UV and white light
(Sole Source Specifications for VersaDoc MP 5000)
- Excites a large variety of fluorophores
- Greater stokes shift for better spectral
discrimination and higher signal-to-noise
- Analysis of colorimetric and densitometry
samples
(Sole Source Specifications for VersaDoc MP 5000)
- Removes electronic background noise from
system
- Corrects for all dead pixels on CCD
- Corrects for all hot pixels on CCD
(Sole Source Specifications for VersaDoc MP 5000)
- Allows variable spatial resolution to
approximately 195 µm.
- Enhanced image uniformity
- Allows high light collection efficiency, ideal
chemiluminescence imaging
- Allows spatial resolution to 150 µm.
- Customers can use any Nikon F-mount lens
with a minimum focus distance less than or
equal to 0.65m for greatest flexibility
- Imaging area of 25 cm x 25 cm
(Sole Source Specifications for VersaDoc MP 5000)
- Faster scan times than laser based point
scanners
(Sole Source Specifications for VersaDoc 5000)
- Accommodates a large number of fluorescent,
chemiluminescent, chemifluorescent, and
colorimetric techniques
- Allows flexibility for future imaging techniques &
applications.
(Sole Source Specifications for VersaDoc MP 5000)
- Reduces background fluorescence close to zero
counts, enhancing signal-to-noise ratio
Benefit
(Sole Source Specifications for VersaDoc MP 5000)
- Acquisition multiple fluorophore colors
- Multiplexing of standards and unknowns
allows greater sample throughput and MW
accuracy
Quantity One® 1-D Analysis
Acquisition Software
Feature
♦ Unlimited copies of
Quantity One Basic
♦ Quantity One Analysis
Software
Benefit
(Sole Source Specifications for VersaDoc MP 5000)
♦ Provided in addition to the full license copy provided with
each instrument. Users can now take advantage of a
number of useful analysis features without the need to
purchase additional licenses (See Bulletin 2908)
♦ One free full version license provided with each system
sold in addition to unlimited copies of Quantity One Basic.
Quantity One provides the user with tools to analyze and
quantitate 1D gels and blots, arrays, and colonies by use
of volume analysis or band analysis. A number of
annotation and display features are available. Quantity
One is compatible with PC and Mac platforms.
♦ Provides greater ease-of-use and automation
♦ Application based software requires no user
knowledge of instrument settings.

♦ Multi-Channel Viewer ♦ Allows users to overlay colorimetric standards and

chemiluminescent samples

♦ 3-D Viewer ♦ Enables better assessment of band or spot features

♦ Quick guides for ♦ Promotes ease-of-use workflow

Printing, Volume, Band,
Colony Counting,
Differential Display,
Phylogenetic Tree, and
VNTR Analyses
♦ Band, Lane, Matching, ♦ Complete menu of reports to fit needs of different users
Image, Phylogenetic,
Similarity Matrix, and
VNTR Reports
♦ Archiving of Band ♦ Saves time on analysis of similar samples
Detection Parameters
and Standards
♦ 17 Pre-saved Bio-Rad ♦ Convenience for faster MW determination
Standards
♦ Mac and PC ♦ The VersaDoc MP 5000 and Quantity One are compatible
compatibility with both Mac OSX and PC platforms
♦ Network versions ♦ Network versions of the Quantity One software is available
as 1, 2, 3, 4, 5, and 10-user network license configurations
♦ CFR compliance ♦ An additional Quantity One software module is available to
assist users with meeting CFR Part 11 compliance
requirements

Page 3 of 3