Académique Documents
Professionnel Documents
Culture Documents
1335-1338, 1997
~ Pergamon © 1997 Elsevier Science Ltd. All rights reserved
Printed in Great Britain
PH:S0031-9422(97)00470-6 0031-9422/97 $17.00+0.00
Abstract--Unknown proteins isolated from mutant tissues of rice (Oryza sativa L.) recovered from inhibitor
selections were subsequently peptide microsequenced. Database searches putatively identified one peptide as
fructose 1,6-bisphosphate aldolase (EC 4.1.2.13). Tissues of mutant rice, PI564784, and wild type (cv Calrose
76) tissues were evaluated for aldolase activity. Total enzyme activities were slightly lower in the mutant than
the control but the differences were not significant. Although the mutant phenotype is for enhanced lysine and
protein, we ascribe the small aldolase differences to physiological adjustments, rather than to D N A modi-
fications of the aldolase gene(s). Homologies of rice peptides with aldolases from a range Of species, as well as
rice cell culture expressed sequence tags (ESTs) are presented. Some amino acids sequences are highly conserved.
The mutant phenotype expressing stress proteins is not likely to be defined by a change in rice aldolases.
© 1997 Elsevier Science Ltd
12
J
a major change in rice aldolases. However, enzyme
activities do not provide information on protein
aggregation and post translational modifications such
43-- as phosphorylation and glycosylation which could
produce modified electrophoretic profiles.
-41 EXPERIMENTAL
Table 1. Fructose-1,6 bisphosphate aldolase activity in crude extracts of seedlings and expanded (Epd) leaves of an in vitro-
derived mutant (PI564784) and Calrose 76 (Cal-76), the source cultivar
CaI-76 Epd Leaf 12.7 0.213 2.86 13.4 0.225 0.256 3.26 0.055
Mnt Epd Leaf 10.6 0.157 1.8 11.4 0.17 0.292 3.10 0.046
Cal-76 Seedling 32.3 0.447 8.73 18.3 0.27 0.389 12.54 0.185
Mut Seedling 26.1 0.383 5.4 15.0 0.22 0.394 10.28 0.151
Fructose 1,6-bisphosphate aldolase in rice 1337
k
I 2 3 4 6 8 7 8 ¢ 10 I 11 12 I5 14 15 18 17 18 19 ~ I
Mutant
1 64
l (MS
$ O6
4 O4
I|
6 62
e 95
? 64
8 4 ,• -/ ~ .. .
. . . . . . . ~ . ~ /
6 95
10 M • / ~ o • v • • K ~ ' ~ /
11 66
12 4 S ~ A " / ~ . . . . . . ~ ' ~ /
1t 6 8 K E " I ~ * Y * " T " ~ * ~ 1
14 6 K K N I ~ A Y * O T " ~ K ~ I
16 47 ~ s A • / ~ - • v * • - ~ ' ~ /
16 6 S O K I I ~ L R * V A " ~ K ~ I
17 10 ~ E O K ! I I ~ L R " V T * ~ K ~ I
18 10 E Q K I /~H R * V A " ~ K ~ I
19 9 P E O K I I~Q R " V A S ~ K ~ I
20 21 22 23 24 25 23 27 28 29 30 / 31 32 33 34 35 36 37 38 39 40 I 41 42 43 44
i
1 84
/
2 85
6 85
4 84
6 82
6 85 I
7 84
6 24
j
6 85
10 74
/il
11 86
1:l 24
I$ 26 T i N V I
14 26 N V /
mC:M
18 67
17 V G S I
T G S I
19 29 V G~I