Cell Biolabs Catalog WEB

TABLE OF CONTENTS
Ordering Information & Support
Cell-Based Assays
TRANSFORMATION
ADHESION
MIGRATION
INVASION
WOUND HEALING
PHAGOCYTOSIS
VIABILITY/DEATH
SENESCENCE
CONTRACTION
ANGIOGENESIS
Stem Cell Research
25
IPS CELL REPROGRAMMING

RETROVIRAL EXPRESSION SYSTEMS
COLONY FORMATION ASSAYS
ALKALINE PHOSPHATASE ASSAYS
FEEDER CELLS
Viral Expression
33
ADENOVIRUS
ADENO-ASSOCIATED
VIRUS (AAV)
LENTIVIRUS
RETROVIRUS
PREMADE VIRUSES
EXPRESSION SYSTEMS
PURIFICATION KITS
TITER KITS
TRANSDUCTION KITS
microRNA Analysis
65
PRECURSOR CLONE COLLECTION

MAMMALIAN EXPRESSION VECTORS
VIRAL EXPRESSION PLASMIDS
FUNCTIONAL REPORTER SYSTEM
KNOCKDOWN ENHANCER
Phone 1-858-271-6500
USA Toll-Free 1-888-CBL-0505
Fax 1-858-271-6514
TABLE OF CONTENTS
Oxidative Stress / Damage
75
PROTEIN OXIDATION/NITRATION
LIPID PEROXIDATION
DNA/RNA DAMAGE AND REPAIR
REACTIVE OXYGEN SPECIES (ROS) ASSAYS
ANTIOXIDANT ASSAYS
Cell Signaling & Protein Biology
97
SMALL GTPASE / G-PROTEIN SIGNALING

KINASE ASSAYS
REPORTER CELLS AND REAGENTS
EPITOPE TAGS
PROTEIN PHOSPHORYLATION
Metabolism Research
111
CHOLESTEROL ASSAYS
APOLIPOPROTEIN ASSAYS
APOLIPOPROTEINS AND LIPOPROTEINS
ANTIBODIES TO APOLIPOPROTEINS
RENAL FUNCTION ASSAYS
Infectious Disease Research
119
VIRUS CORE ANTIGEN ASSAYS

BACTERIAL RAPID DETECTION KITS
Product Index
123
Worldwide Contacts
130
www.cellbiolabs.com
info@cellbiolabs.com
ORDERING INFORMATION & SUPPORT

Placing an Order within the U.S.
Worldwide Technical Support
Our Customer Service representatives are

available Monday through Friday from 8 am
to 5 pm Pacific Time.
Do you have questions about a particular

product before you buy? Do you need help
with a protocol?
Most orders received by 2 pm Pacific Time

will be shipped the same day. We will notify
you immediately of any backordered item.
Our Technical Service Scientists have been

directly involved in the development and
testing of our products, so you get the benefit of their hands-on experience.
We accept VISA, MasterCard and American Express cards. Net 30 day terms may
be offered upon credit approval.
Phone
1 858 271 6500

1 888 CBL 0505 (Toll-Free)
Phone
1 858 271 6500

1 888 CBL 0505 (US Toll-Free)
Fax
1 858 271 6514
E-mail
tech@cellbiolabs.com
Fax
1 858 271 6514
E-mail
orders@cellbiolabs.com
Pricing
Online
www.cellbiolabs.com
Mail
Attn: Customer Service

7758 Arjons Drive
San Diego, CA 92126
Current U.S. prices are available online at

www.cellbiolabs.com, or you may request a
separate price list by e-mail. Prices are subject to change without notice.
For pricing outside the U.S. please contact
your local distributor, which can be found on
the inside back cover of this catalog.
Placing an Order outside the U.S.
Kit Components
Please see our Worldwide Distributors

section on the inside back cover of this catalog. We have a network of global distributors
serving life science researchers in over 70
countries.
Because our kits are QC tested by lot, individual kit components are generally not
available for purchase separately. However,
certain components may be available in
bulk quantities on a custom basis. For more
information please inquire by sending a
message to sales@cellbiolabs.com.
If you dont see your country listed, please

contact our U.S. office.
Phone 1-858-271-6500
Fax 1-858-271-6514
CELL-BASED ASSAYS
Colony Formation Assays
Tumor Cell / Soft Agar Assays

Transformation of normal cells into neoplastic cells results in a population capable of proliferating independently of internal and external signals that normally restrain growth. The
soft agar colony formation assay has traditionally been used to monitor anchorageindependent growth, employing 3-4 weeks of cell growth followed by manual cell counting.
We have advanced the soft agar assay to eliminate tedious manual cell counting, allow
high-throughput drug screening, and enable recovery of transformed cells for downstream
analysis. These advances have also allowed us to develop a unique kit for the separation
of clonogenic cancer cells from normal cells in heterogeneous solid tumors.
CytoSelect 96-Well Cell Transformation AssayTraditional

Soft Agar Colony Formation
Our CytoSelect 96-Well Cell Transformation Assay
(Soft Agar Colony Formation) is suitable for measuring malignant transformation where no downstream
analysis is required. Transformed cells cannot be recovered; however, no manual cell counting is required.
Fast Results: 6-8 days vs. 21 days

Plate Reader Convenience: Eliminates manual
counting
Versatile Format: Designed for 96-well throughput, but can be adapted for 48, 24, 12 or 6-well
With this assay, cells are incubated in a semisolid

agar medium for 6-8 days, then solubilized, lysed and
detected using CyQuant GR dye in a fluorometric
plate reader.
Recent Product Citations
1. Inami, Y. et al. (2011). Persistent activation of Nrf2 through p62
in hepatocellular carcinoma cells. J. Cell Biol. 193(2):275-284.
2. Carnahan, J. et al. (2010). Selective and potent Raf inhibitors
paradoxically stimulate normal cell proliferation and tumor
growth. Mol. Cancer Ther. 9:2399-2410.
3. Liu, F. et al. (2010). Epigenomic alterations and gene expression profiles in respiratory epithelia exposed to cigarette smoke
condensate. Oncogene 29:3650-3664.
4. Iorns, E. et al. (2010). The role of SATB1 in breast cancer
pathogenesis. J. Natl. Cancer Inst. 102(16):1284-1296.
5. Faoro, L. et al. (2010). EphA2 mutation in lung squamous cell
carcinoma promotes increased cell survival, cell invasion, focal
adhesions, and mammalian target of rapamycin activation. J.
Biol. Chem. 285:18575-18585.
6. Rubio, R. et al. (2010). Deficiency in p53 but not retinoblastoma
induced the transformation of mesenchymal stem cells in vitro
and initiates leiomyosarcoma in vivo. Cancer Res. 70:41854194.
7. Li, H. et al. (2009). Lysophosphatidic acid stimulates cell migration, invasion, and colony formation as well as tumorigenesis/
metastasis of mouse ovarian cancer in immunocompetent
mice. Mol. Cancer Ther. 8:1692-1701.
Cell Transformation Assay Principle.
Product Name
Detection
CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony

Formation)
Phone 1-858-271-6500
Size
Catalog Number
1 Plate*
CBA-130
5 Plates*
CBA-130-5
Fluorometric
Fax 1-858-271-6514
CELL-BASED ASSAYS
CytoSelect 96-Well Cell Transformation AssaysAdvanced

Soft Agar with Post-Incubation Cell Recovery
The CytoSelect 96-Well Cell Transformation Assay
(Cell Recovery Compatible) provides a robust system
for screening oncogenes and cell transformation inhibitors. Transformed cells may be recovered for further downstream analysis following colony formation.
Faster Results: 6-8 days vs. 21 days

Cell Recovery: Transformed cells remain viable
for further analysis
counting of cells
Versatile Format: Designed for 96-well throughput, but can be adapted for 48, 24, 12 or 6-well
500
HeLa
450
NIH3T3
400
RFU
350
300
250
200
150
100
50
0
5000
2500
1250
625
313
Cells Seeded/Well
Easy Fluorescence Detection with the CytoSelect Cell Transformation Assay. HeLa and NIH3T3 cells were seeded at various
concentrations and cultured for 6 days. Transformed colonies were
quantified according to the assay protocol.
Cell Transformation Assay Principle. Cell colonies form after a 6

-8 day incubation with agar matrix. Transformed cells can then be
either lysed and detected with a fluorescent dye or recovered and
re-plated.
Product Name

1. Mathew, B. et al. (2011). The novel role of the mu opioid receptor in lung cancer progression: A laboratory investigation.
Anesth. Analg. 112:558-567. (CBA-135)
2. Hirata, H. et al. (2010). Role of secreted Frizzled-related protein3 in human renal cell carcinoma. Cancer Res. 70:1896-1905.
(CBA-135)
3. Hirata, H. et al. (2009). Wnt antagonist gene DKK2 is epigenetically silenced and inhibits renal cancer progression through
apoptotic and cell cycle pathways. Clin. Cancer Res. 15:56785687. (CBA-135)
4. Xie, G. et al. (2009). Acetylcholine-induced activation of M3
muscarinic receptors stimulates robust matrix metalloproteinase
gene expression in human colon cancer cells. Am. J. Physiol.
Gastrointest. Liver Physiol. 296:G755-G763. (CBA-135)
Detection
Size
Catalog Number
1 Plate*
CBA-135
5 Plates*
CBA-135-5
1 Plate*
CBA-140
5 Plates*
CBA-140-5
1 Plate***
CBA-145
5 Plates***
CBA-145-5
Colorimetric
CytoSelect 96-Well Cell Transformation Assay
(Cell Recovery Compatible)
Fluorometric
CytoSelect 384-Well Cell Transformation Assay**
Fluorometric
*Each kit provides sufficient reagent quantities to perform 96, 48, 24, 12, or 6 tests in a 96, 48, 24, 12, or 6-well plate, respectively.
**The 384-well kit does not allow for cell recovery due to small well size.
***Each kit provides sufficient reagents for one or five 384-well plates respectively.
www.cellbiolabs.com
CELL-BASED ASSAYS
CytoSelect 96-Well In Vitro Tumor Sensitivity Assay

The CytoSelect In Vitro Tumor Sensitivity Assay
provides a stringent, anchorage-independent model
for chemosensitivity testing and possible anticancer
drug screening. The assay uses a soft agar matrix to
promote the colony formation of neoplastic cells in
about a week. Cells are quantified using a standard
ELISA plate reader.
Fast Results: 6-8 days
In Vivo Simulation: Resembles a threedimensional cell environment
counting
1. Itamochi, H. et al. (2011). Inhibiting the mTOR pathway synergistically enhances cytotoxicity in ovarian cancer cells induced
by etoposide through upregulation of c-Jun. Clin. Cancer Res.
17:4742-4750.
2. Kang, D.W. et al. (2010). Phospholipase D1 drives a positive
feedback loop to reinforce the Wnt/-catenin/TCF signaling
axis. Cancer Res. 70:4233-4242.
Tumor Sensitivity Assay Principle.
0.60
OD 570nm
0.50
0.40
0.30
0.20
0.10
0.00
-11
-9
-7
-5
-3
Log [5-FU] (M)

Inhibition of HeLa Cell Anchorage-Independent
Growth by Taxol. HeLa cells were cultured for 7
days in the absence (top) or presence (bottom) of 1
nM Taxol according to the assay protocol.
Product Name
Detection
CytoSelect 96-Well In Vitro Tumor Sensitivity Assay
Phone 1-858-271-6500
Inhibition of HeLa Cell Transformation by 5-Fluorouracil. HeLa

cells were seeded at 5000 cells/well and cultured 7 days at various
5-FU concentrations. Cell transformation was determined according to the assay protocol. IC50 value of 5-Fluorouracil on HeLa cell
anchorage-independent growth was determined to be ~ 1 M.
Size
Catalog Number
96 Assays
CBA-150
5 x 96 Assays
CBA-150-5
Colorimetric
Fax 1-858-271-6514
CELL-BASED ASSAYS
CytoSelect Clonogenic Tumor Cell Isolation Kit

Clean separation of clonogenic tumor cells from normal cells is critical for proper analysis of disease state
progression. Due to the heterogeneity of many tumors, however, isolation of homogenous tumor cell
populations can be difficult.
The CytoSelect Clonogenic Tumor Cell Isolation Kit
uses a proprietary semisolid agar medium to facilitate
colony formation by cells from solid tumors.
Colonies are grown in either a 6-well plate or a 35
mm dish. The colonies are then isolated away from
single cells by size filtration.
Efficient: Easily eliminates single cells from

clonogenic tumor cell population
Versatile: In addition to solid tumors, has
potential use in isolating tumor stem cells
Clonogenic Colony Formation, Isolation and Re-plating. A:

Clonogenic colony formation (red arrows) and single cells (black
arrows) after 7 day incubation. B: Isolation of clonogenic colonies
from single cells. C: Re-plated clonogenic colonies after 3 days (no
trypsinization). D: Re-plated clonogenic colonies 1 day after
trypsinization.
Clonogenic Tumor Cell Isolation Procedure.
Product Name
CytoSelect Clonogenic Tumor Cell Isolation Kit
www.cellbiolabs.com
Size
Catalog Number
5 Preps
CBA-155
25 Preps
CBA-155-5
CELL-BASED ASSAYS
Cell Adhesion
Cell Adhesion Assays

Cell adhesion is a complex mechanism involved in a variety of processes including cell migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells can adhere
to the ECM, forming complexes with cytoskeletal components, or to the endothelium.
Our CytoSelect Cell Adhesion Assays quantify adhesion of cells using a microplate
reader or fluorometer; no manual cell counting is required.
CytoSelect ECM Cell Adhesion Assays

The CytoSelect ECM Cell Adhesion Assays provide
a quantitative method for evaluation of cell adhesion.
The 48-well plate is precoated with your choice of
substrate. Cells are seeded onto the substrate; adherent cells attach, while non-adherent cells are washed
away. Adherent cells can be quantified on a standard
plate reader or fluorometer.
Quantitative: Measure results in a colorimetric or
fluorescence plate reader
Flexible: Uniform substrate layer of your choice of
Collagen I, Collagen IV, Fibrinogen, Fibronectin, or
Laminin; or choose the ECM array which contains
all 5 ECM proteins
1. Cervera, A.M. et al (2008). Cells silenced for SDHB expression display characteristic features of the tumor phenotype.
Cancer Res. 68:4058-4067. (CBA-050 and CBA-070)
2. Miao, H. et al. (2008). Gene expression and functional studies of the optic nerve head astrocyte transcriptome from
normal African Americans and Caucasian Americans donors. PLoS One 3(8):E2847. (CBA-060)
Product Name
CytoSelect 48-Well Cell Adhesion Assay, ECM Array
(Contains one row each of Collagen I, Collagen IV, Fibrinogen,
Fibronectin, and Laminin)
BSA
Vitronectin
Laminin
Fibronectin
Collagen IV
Collagen I
MDA-231 HT-1080 HEK293
CytoSelect 48-well Cell Adhesion Assay. Serum starved cells
from three different cell lines were allowed to attach to the ECMcoated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells
were stained according to the assay protocol.
Detection
Size
Catalog Number
Colorimetric
48 Assays
CBA-070
Fluorometric
48 Assays
CBA-071
Colorimetric
48 Assays
CBA-052
Fluorometric
48 Assays
CBA-053
Colorimetric
48 Assays
CBA-060
Fluorometric
48 Assays
CBA-061
CytoSelect 48-Well Cell Adhesion Assay, Collagen I
CytoSelect 48-Well Cell Adhesion Assay, Collagen IV

Colorimetric
48 Assays
CBA-058
Fluorometric
48 Assays
CBA-059
Colorimetric
48 Assays
CBA-050
Fluorometric
48 Assays
CBA-051
Colorimetric
48 Assays
CBA-056
Fluorometric
48 Assays
CBA-057
CytoSelect 48-Well Cell Adhesion Assay, Fibrinogen
CytoSelect 48-Well Cell Adhesion Assay, Fibronectin
CytoSelect 48-Well Cell Adhesion Assay, Laminin
10
Phone 1-858-271-6500
Fax 1-858-271-6514
Cell Adhesion
CELL-BASED ASSAYS
CytoSelect Leukocyte Endothelium Adhesion Assays

The CytoSelect Leukocyte Endothelium Adhesion
Assays provide a robust system for the quantitative
determination of interactions between leukocytes
and endothelium. Adherent cells can be quantified
on a fluorescence plate reader.

1. Xue, J. et al. (2009). NFkB regulates thrombin-induced ICAM1 gene expression in cooperation with NFAT by binding to the
intronic NFkB site in the ICAM-1 gene. Physiol. Genomics
38:42-53. (CBA-210)
2. Hernandez, L. et al. (2010). Activation of NF-kB signaling by
inhibitor of NF-kB Kinase increases aggressiveness of ovarian cancer. Cancer Res. 70:4005-4014. (CBA-215)
3. Gu, L. et al. (2010). Stat5 promotes metastatic behavior of
human prostate cancer cells in vitro and in vivo. Endocr. Relat. Cancer 17:481-493. (CBA-215)
140
120
RFU
100
80
Ctrl
60
PMA
40
20
0
25,000
50,000
100,000
THP-1 Cells
CytoSelect Leukocyte-endothelium Adhesion Assay

Principle.
Product Name
Human Monocytic THP-1 Adhesion to HUVEC Monolayer

Using the CytoSelect Leukocyte-endothelium Adhesion
Assay. HUVEC monolayer was treated with 1 M PMA for 12 hrs.
LeukoTracker labeled THP-1 cells were allowed to attach to
HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified as described in the assay protocol.
Detection
Size
Catalog Number
CytoSelect Leukocyte-Endothelium Adhesion Assay
Fluorometric
96 Assays
CBA-210
CytoSelect Leukocyte-Epithelium Adhesion Assay
Fluorometric
96 Assays
CBA-211
CytoSelect Tumor-Endothelium Adhesion Assay
Fluorometric
96 Assays
CBA-215
Product Name
Detection
Size
Catalog Number
2 Chips
CBA-003
CytoSelect 8-Channel ECM Microfluidic Biochips
Microscopy
10 Chips
CBA-003-5
CytoSelect Microfluidic Biochips

CytoSelect 8-Channel Microfluidic Biochips provide
an environment that closely mimics in vivo shear
stresses, resulting in more physiologically relevant
cell adhesion data. The Biochips are self-contained
units containing 8 channels with inlet/outlet ports at
both ends of each channel. After adding cell samples,
a syringe pump set to the proper flow rate applies the
appropriate shear stress to the channel.
CytoSelect 8-Channel Endothelial Microfluidic Biochips
2 Chips
CBA-004
10 Chips
CBA-004-5
Microscopy
www.cellbiolabs.com
11
CELL-BASED ASSAYS
Cell Migration / Invasion
Cell Migration & Invasion Assays

Cell migration and invasion are highly integrated, multi-step processes and play important
roles in the progression of various diseases including cancer, atherosclerosis and arthritis.
Our cell migration assays are provided in two formats: 2-Dimensional Gap Closure and
Boyden Chamber. Each format has its own advantages and applications. Use the information below to help choose the best format for your cell migration experimental goals.
Cell invasion assays are provided in the Boyden Chamber format.
Cell Migration Format Selection Guide

2D Gap Closure Assays
(p. 13)
Boyden Chamber Assays

(p. 14-19)
Qualitative or Quantitative
Quantitative
Endpoint or Real Time
Endpoint
Microscopy
Plate Reader
No
Yes
Relative Sensitivity
Good
Fair
Adaptability to Automation
Good
Poor
Universal
Choose membrane pore size

based on cell type/size
Type of Analysis
Detection Time
Detection Method
Chemoattractant Gradient
Cell Compatibility
Example Results using 2D Gap Closure Assay.
12
Phone 1-858-271-6500
Example of Boyden Chamber Assay Principle.
Fax 1-858-271-6514
CELL-BASED ASSAYS
Radius Cell Migration Assays (2D Gap Closure)

Radius Cell Migration Assays provide a unique alternative to the traditional Boyden Chamber migration
assay. Radius assays allow you to measure cell migration at endpoint or in real time, and are ideal for
time course migration studies.
Radius Cell Migration Assays use a cell culture
plate containing a proprietary, carefully-defined biocompatible hydrogel (Radius gel) spot centralized
at the bottom of each well. Cells seeded in the well
will attach everywhere except on the Radius gel spot,
creating a cell-free zone. Once cells attach, the Radius gel is removed and migration of cells across the
cell-free zone begins. The gel removal step allows
synchronization of a zero time point to facilitate wellto-well comparisons.
With Radius Cell Migration Assays, there are no
cell culture inserts; so you dont need to worry about
which pore size to choose for your cell type. Any adherent cell may be used in the assay.
Radius assays are supplied in 24-well, 96-well and
384-well formats. In addition, the 24-well assays are
provided with your choice of coatings for proper cell
attachment:
Uncoated
Collagen I-coated
Fibronectin-coated
Laminin-coated
ECM Array with 6 wells of each of the above
(uncoated, Collagen I, Fibronectin, Laminin); ideal
if you are unsure which ECM protein may provide
the best cell attachment
Product Name
Assay Principle for the Radius Cell Migration Assays.

Detection
Radius 24-Well Cell Migration Assay
Size
Catalog Number
24 Assays
CBA-125
5 x 24 Assays
CBA-125-5
Microscopy
Radius 24-Well Cell Migration Assay (Collagen I Coated)
Microscopy
24 Assays
CBA-125-COL
Radius 24-Well Cell Migration Assay (Fibronectin Coated)
Microscopy
24 Assays
CBA-125-FN
Radius 24-Well Cell Migration Assay (Laminin Coated)
Microscopy
24 Assays
CBA-125-LN
Radius 24-Well Cell Migration Assay (ECM Array Coated)
Microscopy
24 Assays
CBA-125-ECM
96 Assays
CBA-126
Microscopy
5 x 96 Assays
CBA-126-5
384 Assays
CBA-127
5 x 384 Assays
CBA-127-5
Microscopy
www.cellbiolabs.com
13
CELL-BASED ASSAYS
CytoSelect Cell Migration and Invasion Assays (Boyden Chamber)

The Boyden Chamber has been extensively used and
widely published as a tool for measuring cell migration and cell invasion in vitro. Our CytoSelect Cell
Migration and Invasion Assays use this well-cited
method to quantify cell migration and invasion with no
manual cell counting required. Migratory or invasive
cells are quantified using a colorimetric or fluorometric
plate reader.
Cell migration may take on various forms and behaviors depending on the type and location of cells. Such
subclasses of cell migration include chemotaxis, haptotaxis, and transmigration. Use the chart below to
compare the various subclasses of cell migration as
well as cell invasion, which will help you choose the
assay best suited to your experimental goals.
Typical Well Setup for Boyden Chamber Assay.
Boyden Chamber Assay Selection Guide

Assay
Chemotaxis
(p. 15)
Haptotaxis
(p. 16)
Definition
Migration of cells
toward a chemoattractant
(chemical signal) in the cells
surrounding environment
Migration of cells along a

gradient of cellular adhesion
sites or extracellular matrixbound chemoattractants
Migration of cells through the

Transmigration
vascular endothelium toward a
(p. 17)
chemoattractant
Invasion
(p. 18-19)
14
Movement of cells through the

3D extracellular matrix into
neighboring tissues; includes
ECM degradation and
proteolysis
Phone 1-858-271-6500
Cell
Types
Pore
Size
Insert
Coating
Assay Formats
Neutrophils
Leukocytes
3 m
None
24-Well
96-Well
Lymphocytes
Monocytes
Macrophages
5 m
None
24-Well
96-Well
Fibroblasts
Endothelial Cells
Epithelial Cells
Tumor Cells
8 m
None
24-Well
96-Well
Astrocytes
12 m
Slow-moving Cells
None
24-Well
Collagen I
(bottom)
24-Well
Fibronectin
(bottom)
24-Well
Fibroblasts
Endothelial Cells
Epithelial Cells
8 m
Leukocytes
3 m
None
24-Well
Tumor Cells
8 m
None
24-Well
ECM Matrix
(top)
24-Well
96-Well
Collagen I
(top)
24-Well
96-Well
Laminin I
(top)
24-Well
96-Well
Fibroblasts
Endothelial Cells
Epithelial Cells
Tumor Cells
8 m
Fax 1-858-271-6514
CELL-BASED ASSAYS
CytoSelect Cell Migration AssaysChemotaxis

CytoSelect Cell Migration Assays are ideal for
measuring chemotaxis. The kits utilize polycarbonate
membrane inserts in 24-well or 96-well plates. Inserts
are available with 4 different pore sizes to accommodate a variety of cell types.
Fast Results: Visualize chemotaxis in less than
6 hours with most cell types
Flexible: Bottoms of membrane inserts are uncoated to allow use with any chemoattractant
Higher Throughput: 96-well format available for
fluorescence plate readers
Assay Principle for the CytoSelect Cell Migration Assay.

Product Name
CytoSelect 24-Well Cell Migration Assay
0% FBS
10% FBS
Migration of Human Fibrosarcoma HT-1080 Cells. Cells were

seeded at 30,000 cells per well of a 24-well plate and allowed to
migrate toward 10% FBS for 4 hours. Migratory cells were stained
(above) and quantified in a fluorescence plate reader (data not
shown).
1. Tabata, C. et al. (2010). Novel clinical role of angiopoietin-1 in
malignant pleural mesothelioma. Eur. Respir. J. 36(5):1099-1105.
(CBA-100)
2. Awasthi, N. et al. (2009). Endothelial monocyte activating polypeptide II interferes with VEGF-induced proangiogenic signaling.
Laboratory Investigation 89(1):38-46. (CBA-100)
3. Igarashi, J. et al. (2009). Transforming growth factor-1 downregulates caveolin-1 expression and enhances sphingosine 1phosphate signaling in cultured vascular endothelial cells. Am. J.
Physiol. Cell Physiol. 297:C1263-C1274. (CBA-100)
4. Izhak, L. et al. (2010). Predominant expression of CCL2 at the
tumor site of prostate cancer patients directs a selective loss of
immunological tolerance to CCL2 that could be amplified in a
beneficial manner. J. Immunol. 184:1092-1101. (CBA-101)
5. Shynlova, O. et al. (2008). Monocyte chemoattractant protein-1
(CCL-2) integrates mechanical and endocrine signals that mediate term and preterm labor. J. Immunol. 181:1470-1479. (CBA102)
6. Chatterjee, S. et al. (2009). Site-specific carboxypeptidase B1
tyrosine nitration and pathophysiological implications following its
physical association with nitric oxide synthase-3 in experimental
sepsis. J. Immunol. 183:4055-4066. (CBA-104)
7. Christophi, G. et al. (2008). Modulation of macrophage infiltration
and inflammatory activity by the phosphatase SHP-1 in virusinduced demyelinating disease. J. Virol. 83:522-539. (CBA-105)
8. Saher, H. et al. (2010). Red wine consumption improves in vitro
migration of endothelial progenitor cells in young healthy individuals. Am. J. Clinical Nutrition 92:161-169. (CBA-106)
Pore Size
Detection
Size
Catalog Number
3 m
Fluorometric
12 Assays
CBA-103
5 m
Fluorometric
12 Assays
CBA-102
Colorimetric
12 Assays
CBA-100
Fluorometric
12 Assays
CBA-101
Colorimetric
12 Assays
CBA-107
Fluorometric
12 Assays
CBA-108
3 m
Fluorometric
96 Assays
CBA-104
5 m
Fluorometric
96 Assays
CBA-105
8 m
Fluorometric
96 Assays
CBA-106
8 m
12 m
CytoSelect 96-Well Cell Migration Assay
www.cellbiolabs.com
15
CELL-BASED ASSAYS
CytoSelect Cell Migration AssaysHaptotaxis

Haptotaxis describes the migration of cells toward a
gradient of immobilized extracellular matrix. The
CytoSelect Cell Haptotaxis Assays are ideal for
determining the migratory properties of cells. The
kits utilize polycarbonate membrane inserts with an
8 m pore size in a 24-well plate.
The undersides of the inserts are coated with either
Collagen or Fibronectin. The 8 m pore size in the
membrane inserts is ideal for epithelial cells, endothelial cells, fibroblasts, and other cells of similar
size. The membrane serves as a barrier that allows
discrimination of migratory cells from non-migratory
cells.
Fast Results: Visualize cell haptotaxis in

less than 6 hours with most cell types
Convenient: Membrane inserts pre-coated
on the underside with either Collagen I or
Fibronectin
Versatile: Useful with a variety of cell types
including epithelial cells, endothelial cells,
and fibroblasts*
*For leukocyte migration a 3 m pore size is recommended.
See our CytoSelect Cell Migration Assays (previous page)
or the Leukocyte Transmigration Assay (next page).
Recent Product Citation

Kamiya, K. et al. (2007). Protein Kinase C delta activated adhesion
regulates vascular smooth muscle cell migration. J. Surg. Res.
141:91-96. (CBA-100-COL)
BSA
Collagen I
Fibronectin
0%
FBS
0.5%
FBS
CytoSelect 24-well Cell Haptotaxis Assay. MDA-231 cells

were seeded at 150,000 cells/well and allowed to migrate toward
FBS for 4 hrs. Migratory cells, found on the bottom of the migration
membrane, were stained according to the assay protocol.
Assay Principle for the CytoSelect Cell Haptotaxis Assay.
Product Name
Detection
Size
Catalog Number
Colorimetric
12 Assays
CBA-100-COL
Fluorometric
12 Assays
CBA-101-COL
Colorimetric
12 Assays
CBA-100-FN
Fluorometric
12 Assays
CBA-101-FN
CytoSelect 24-Well Cell Haptotaxis Assay, Collagen I-coated
CytoSelect 24-Well Cell Haptotaxis Assay, Fibronectin-coated
16
Phone 1-858-271-6500
Fax 1-858-271-6514
CELL-BASED ASSAYS
CytoSelect Cell Migration AssaysTransmigration

Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. The
CytoSelect Cell Transmigration Assays provide a
robust system for the quantitation of transmigrations
and interactions between endothelium and cancer
cells. Migratory cells are quantified via fluorometer.

1. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell
growth. Cancer Res. 68:6752-6761. (CBA-212)
2. Xu, Z. et al. (2010). Role of pancreatic stellate cells in pancreatic
cancer metastasis. Am. J. Pathol. 177:2585-2596. (CBA-216)
3. Yang, H. and H.E. Grossniklaus (2010). Constitutive overexpression of pigment epithelium derived factor inhibition of ocular
melanoma growth and metastasis. Invest. Ophthalmol. Vis. Sci.
51:28-34. (CBA-216)
The Leukocyte Adhesion and Transmigration Cascade.
200
RFU
160
120
80
40
0
0
10,000
20,000
30,000
40,000
50,000
Cell Number
Quantitation of Human Monocytic THP-1. LeukoTracker
labeled THP-1 cells were titrated in 1X PBS, then lysed with 2X
lysis buffer. Fluorescence was quantified as described in the
assay protocol.
Assay Principle for the CytoSelect Leukocyte

Transmigration Assay.
Product Name
Pore Size
Detection
Size
Catalog Number
CytoSelect Leukocyte Transmigration Assay
3 m
Fluorometric
24 Assays
CBA-212
CytoSelect Tumor Transendothelial Migration Assay
8 m
Fluorometric
24 Assays
CBA-216
www.cellbiolabs.com
17
CELL-BASED ASSAYS
CytoSelect Cell Invasion Assays

Tumor cell invasion into surrounding normal tissue
contributes to the morbidity of cancers. The CytoSelect Cell Invasion Assays use precoated inserts to
assay invasive properties of tumor cells in 24-well or
96-well plates. The coated layer serves to distinguish
invasive cells from non-invasive cells. Plates are precoated with either basement membrane matrix (from
EHS mouse sarcoma cells), Collagen I or Laminin I.
Quantitative: Measure results in a colorimetric or fluorescence plate reader

Flexible: Uniform protein matrix layer of your
choice of basement membrane (from mouse
tumor cells), Collagen I, or Laminin I
Versatile: Characterize both the invasive and
migratory properties of your cells with a Cell
Migration / Invasion Combo Kit (next page)
CytoSelect Cell Invasion Assay Principle.

1. Zhang, L. et al. (2011). MicroRNA-1258 suppresses breast cancer brain metastasis by targeting heparanase. Cancer Res.
71:645-654. (CBA-110)
2. Coon, B. et al. (2010). The epsin family of endocytic adaptors
promotes fibrosarcoma migration and invasion. J. Biol. Chem.
285:33073-33081. (CBA-110)
(CBA-110)
4. Ji, H. et al (2007). LKB1 modulates lung cancer differentiation
and metastasis. Nature 48:807-810. (CBA-110, CBA-111, CBA112)
5. Eckstein, N. et al. (2009). Hyperactivation of the insulin-like
growth factor receptor I signaling pathway is an essential event
for cisplatin resistance of ovarian cancer cells. Cancer Res.
69:2996-3003. (CBA-112)
6. Lam, K.K.W. et al. (2009). Glycodelin-A as a modulator of trophoblast invasion. Hum. Reprod. 24:2093-2103. (CBA-112)
7. Neil, J.R. et al. (2008). Cox-2 inactivates Smad signaling and
enhances EMT stimulated by TGF through a PGE2-dependent
mechanism. Carcinogenesis 29:2227-2235. (CBA-112)
8. Thal, D.R. et al. (2008). Expression of coronin-3 (coronin-1C) in
diffuse gliomas is related to malignancy. J. Pathol. 214:415424. (CBA-112)
18
Phone 1-858-271-6500
Human Fibrosarcoma HT-1080 Laminin I Cell Invasion. HT1080 and NIH3T3 (negative control) were seeded at 200,000 cells/
well and allowed to invade toward FBS for 24 hrs. Invasive cells on
the membrane bottom were stained (top and center) and quantified
at OD 560nm after extraction (data not shown).
Fax 1-858-271-6514
CELL-BASED ASSAYS
CytoSelect Cell Invasion Assays, continued

2
HT-1080
1.6
OD 560nm
NIH3T3
1.2
0.8
0.4
0
NIH3T3
HT-1080
HT-1080 +
Cytochalasin D
Effects of Cytochalasin D on Invading Cells using the CytoSelect 24-well Cell Invasion Assay (CBA-110). HT-1080 and NIH3T3
cells (negative control) were seeded at 300,000 cells/well and allowed to invade toward 10% FBS for 24 hrs, in the presence or absence of 2
M Cytochalasin D. Invasive cells, on the bottom of the invasion membrane, were stained (left) and then quantified at OD 560 nm after extraction using a standard plate reader (right).
Product Name
Detection
Size
Catalog Number
Colorimetric
12 Assays
CBA-110
Fluorometric
12 Assays
CBA-111
Colorimetric
12 Assays
CBA-110-COL
Fluorometric
12 Assays
CBA-111-COL
Colorimetric
12 Assays
CBA-110-LN
Fluorometric
12 Assays
CBA-111-LN
CytoSelect 96-Well Cell Invasion Assay, Basement Membrane
Fluorometric
96 Assays
CBA-112
CytoSelect 96-Well Cell Invasion Assay, Collagen I
Fluorometric
96 Assays
CBA-112-COL
CytoSelect 96-Well Cell Invasion Assay, Laminin I
Fluorometric
96 Assays
CBA-112-LN
CytoSelect 24-Well Cell Invasion Assay, Basement Membrane
CytoSelect 24-Well Cell Invasion Assay, Collagen I
CytoSelect 24-Well Cell Invasion Assay, Laminin I
CytoSelect Cell Migration / Invasion Assay Combo Kits

Our CytoSelect Cell Migration / Invasion Assay Combo Kits allow you to characterize both the migratory and
invasive properties of your cells. Each 24-well combo kit provides sufficient reagents to perform 12 migration
plus 12 invasion assays, while the 96-well combo kit allows you to perform 96 migration plus 96 invasion assays.
The invasion plate provided contains basement membrane-coated inserts.
1. Shin, S.Y. et al. (2010). TNFalpha-exposed bone marrow-derived mesenchymal stem cells promote locomotion of MDA-MB-231 breast
cancer cells through transcriptional activation of CXCR3 ligand chemokines. J. Biol. Chem. 285:30731-30740. (CBA-100-C)
2. Gobeil, S. et al. (2008). A genome-wide shRNA screen identifies GAS1 as a novel melanoma metastasis suppressor gene. Genes Dev.
22(21):2932-2940. (CBA-101-C)
3. Axlund, S.D. et al. (2010). HOXC8 inhibits androgen receptor signaling in human prostate cancer cells by inhibiting SRC-3 recruitement to
direct androgen target genes. Mol. Cancer Res. 8:1643-1655. (CBA-106-C)
4. Alfano, R.W. et al. (2009). Matrix metalloproteinase-activated anthrax lethal toxin inhibits endothelial invasion and neovasculature formation during in vitro morphogenesis. Mol. Cancer Res. 7:452-461. (CBA-106-C)
Product Name
Pore Size
CytoSelect 24-Well Cell Migration / Invasion Combo Kit

CytoSelect 96-Well Cell Migration / Invasion Combo Kit
Detection
Size
Catalog Number
Colorimetric
2 x 12 Assays
CBA-100-C
Fluorometric
2 x 12 Assays
CBA-101-C
Fluorometric
2 x 96 Assays
CBA-106-C
8 m
8 m
www.cellbiolabs.com
19
CELL-BASED ASSAYS
CytoSelect 24-Well Wound Healing / Cell Migration Assay

Wound healing assays are useful for studying tissue
matrix remodeling, regulation of cytoskeletal structure, and cell proliferation and migration rates of different cells and culture conditions. Traditional wound
healing assays are performed by making a scratch
across a confluent cell monolayer to create an open
gap, mimicking a wound. Such scratch assays,
however, lack a consistently defined wound area
and result in high inter-sample variation.
Highly Accurate: More consistent results well-towell compared to homemade scratch assays
Versatile: Measure cell migration, cell proliferation, and wound closure
Inert Material: No residues from inserts to impede
cell migration or proliferation
Percent Wound Closure
Our CytoSelect 24-Well Wound Healing Assay

provides a more consistent method to measure cell
migration across a wound field gap in vitro. Proprietary treated inserts generate a consistently defined 0.9mm gap between the cells. Cells can then
be treated and monitored for proliferation or migration across the wound field by imaging samples at
fixed time points or time-lapse microscopy.
0%

Tao, Y. et al. (2011). Treatment of breast cancer cells with DNA
demethylating agents leads to a release of Pol II stalling at genes
with DNA-hypermethylated regions upstream of TSS. Nucleic Acid
Res. 10.1093/nar/gkr611.
50%
100%
CytoSelect 24-well Wound Healing Assay Principle.
20
Wound Closure of STO Cells. STO cells (mouse MEF) were cultured in the provided plate with inserts in place for 24 hours until a
monolayer formed. Inserts were then removed to begin the assay.
Cells were monitored at various time points and stained according
to the assay protocol.
Product Name
Detection
CytoSelect 24-Well Wound Healing Assay
Microscopy
Phone 1-858-271-6500
Size
Catalog Number
24 Assays
CBA-120
5 x 24 Assays
CBA-120-5
Fax 1-858-271-6514
Phagocytosis
CELL-BASED ASSAYS
CytoSelect 96-Well Phagocytosis Assays

Phagocytosis may be assayed by measuring the engulfing of a cell substrate such as an erythrocyte
(RBC) or Zymosan particle. Traditional phagocytosis
assays involve manually counting the engulfed substrates under a microscope. This process is tedious
and time-consuming, can be somewhat inaccurate,
and is not amenable to high throughput.
CytoSelect 96-Well Phagocytosis Assays are more
accurate, high-throughput alternatives to the standard
phagocytosis assay. The assays may be adapted for
use in 48-well and 24-well plates if desired.
1. Lee, J.K. et al. (2010). Regulator of G-protein signaling-10 negatively regulates NF-kB in microglia and neuroprotects dopaminergic neurons in hemiparkinsonian rats. J. Neurosci.
31:11879-11888. (CBA-220)
2. Winnicka, B. et al. (2010). CD13 is dispensible for normal hematopoiesis and myeloid cell functions in the mouse. J. Leukoc.
Biol. 88(2):347-359. (CBA-220)
Assay Principle for the CytoSelect 96-Well Phagocytosis

Assay (Zymosan).
Assay Principle for the CytoSelect 96-Well Phagocytosis

Assay (Red Blood Cell).
Product Name
Particle Engulfment with the CytoSelect 96-Well

Phagocytosis Assay (Zymosan).
Detection
Size
Catalog Number
CytoSelect 96-Well Phagocytosis Assay (Red Blood Cell)
Colorimetric
96 Assays
CBA-220
CytoSelect 96-Well Phagocytosis Assay (Zymosan)
Colorimetric
96 Assays
CBA-224
www.cellbiolabs.com
21
CELL-BASED ASSAYS
Cell Viability / Death
CytoSelect Cell Viability and Cytotoxicity Assay

Cell viability characteristics include cellular metabolic activity and cell membrane integrity. Our CytoSelect Cell Viability and Cytotoxicity Assay provides both a colorimetric and fluorometric format for
monitoring cell viability via metabolic activity.
Live cells are detected with MTT (colorimetric detection) or Calcein AM (fluorometric); dead cells are
detected with EthD-1 reagent (fluorometric). All 3
detection reagents are included, as well as Saponin,
a cell death initiator. Cells may be treated with compounds or agents that affect cell viability. This kit is
suitable for eukaryotic cells, not bacteria or yeast.
Versatile: Detect live and dead cells by microscopy, colorimetric or fluorescence plate reader, or
flow cytometry
Quantitative: Measure live and dead cells on a
fluorescence plate reader; live cells may also be
quantified on a standard microplate reader
Product Name
CytoSelect Cell Viability and Cytotoxicity Assay Kit
Viability of Human Foreskin Fibroblasts. BJ-TERT cells were

seeded at 50,000 cells/well and allowed to culture for 24 hours.
Cells were then treated with and without Saponin. All cells were
then stained with Calcein AM and EthD-1. Top: Cells without
Saponin treatment. Bottom: Cells with Saponin treatment. Left:
Calcein AM staining. Right: EthD-1 staining.
Detection
Size
Catalog Number
Colorimetric /
Fluorometric
1 plate*
CBA-240
*Each kit provides sufficient reagent quantities to perform 24 or 96 assays in a 24-well or 96-well plate respectively.
CytoSelect Anoikis Assays

This assay allows you to quantify and monitor anoikis
in cells using a Poly-HEMA precoated plate. Live
cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric), both included with the kit.
Dead cells are detected with EthD-1 reagent.
Versatile: Detect live and dead cells by microscopy, fluorescence, or flow cytometry
Quantitative: Measure live and dead cells on a
fluorescence plate reader; live cells may also be
quantified on a standard microplate reader
Anoikis of Human Fibroblast BJ-TERT Cells. 50,000 cells/well

were seeded in a control plate (left) and a Poly-HEMA coated plate
(right) and cultured for 24 hours. Cells on the control plate were
stained with Calcein AM. Cells on the Poly-HEMA coated plate
were stained with EthD-1.

1. Sisto, M. et al. (2009). Fibulin-6 expression and anoikis in human salivary gland epithelial cells: implications in Sjogren's syndrome.
Int. Immunol. 21:303-311. (CBA-080)
2. Liu, H. et al (2008). Cysteine-rich protein 61 and connective tissue growth factor induce de-adhesion and anoikis of retinal pericytes.
Endocrinology 149:1666-1677. (CBA-080)
Product Name
22
Detection
Size
Catalog Number
CytoSelect 24-Well Anoikis Assay
Colorimetric /
Fluorometric
24 Assays
CBA-080
CytoSelect 96-Well Anoikis Assay
Colorimetric /
Fluorometric
96 Assays
CBA-081
Phone 1-858-271-6500
Fax 1-858-271-6514
Senescence, Cell Contraction
CELL-BASED ASSAYS
Cellular Senescence Assays

Senescence Associated (SA) -galactosidase is a common biochemical marker of cellular senescence. Cells
expressing such markers have been identified in vivo in tissues. SA -Gal catalyzes hydrolysis of X-gal, which
produces a blue color in senescent cells.
The results may be seen using our SA -gal Staining
Kit or by flow cytometry using our Quantitative Cellular Senescence Assay. For higher throughput, quantify cells on a fluorometric plate reader using our 96Well Cellular Senescence Activity Assay.

Malhotra, D. et al. (2010). Global mapping of binding sites for Nrf2
identifies novel targets in cell survival response through ChIP-Seq
profiling and network analysis. Nucleic Acid Res. 10.1093/nar/
gkq212. (CBA-231)
Product Name
Cellular Senescence Assay Kit (SA -gal Staining)
Detection
Size
Catalog Number
Light Microscopy
50 Assays
CBA-230
Fluorometric
Plate Reader
120 Assays
CBA-231
Fluorescence Microscopy / Flow Cytometry
10 Assays
CBA-232
96-Well Cellular Senescence Assay (SA -gal Activity)

Quantitative Cellular Senescence Assay (SA -gal)
Cell Contraction Assay (Collagen-Based)

Wound healing is comprised of epithelialization, connective tissue deposition, and contraction. The contraction
process is believed to be mediated by specialized fibroblasts (myofibroblasts). 3D collagen gels have been
widely used in fibroblast contraction studies.
Our Cell Contraction Assay provides a simple system
to assess cell contractivity and to screen for cell contraction mediators. The system uses a 3D collagen
matrix to measure changes in the collagen gel size.
An optional contraction inhibitor is provided.

Schell, C. et al. (2010). 15-deoxy-delta12-14-prostaglandin-J2
induces hypertrophy and loss of contractility in human testicular
peritubular cells: implications for human male fertility. Endocrinology 151:12571268. (CBA-201)
Contraction (mm)
Control
BDM
5
4
3
2
1
0
0
50
100
150
Time (min)
Collagen-Based Cell Contraction Assay Principle.
Product Name
Cell Contraction Assay
Contraction Inhibition by BDM. 5 x 105 COS-7 cells in 0.5 mL

collagen gel lattice were cultured for 2 days. Before initiation of
contraction, cells were pretreated with 10mM BDM for 1 hour.
The change in gel diameter (mm) was measured with a ruler at
various times following release.
Detection
Size
Catalog Number
Light Microscopy
24 Assays
CBA-201
www.cellbiolabs.com
23
CELL-BASED ASSAYS
Angiogenesis, Autophagy
Endothelial Tube Formation (In Vitro Angiogenesis) Assay

For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor
cells. These cells proliferate to form new blood vessels.
Our Endothelial Tube Formation Assay provides
an easy, robust system to assess angiogenesis
in vitro. The assay uses an ECM gel matrix derived from mouse sarcoma cells; this matrix very
closely resembles an in vivo basement membrane environment.
1. Kimura, T. et al. (2011). P2y5/LPA6 attenuates LPA1-mediated
VE-cadherin translocation and cell-cell dissociation through
G12/13 protein-Src-Rap1. Cardiovasc. Res. 10.1093/cvr/cvr154.
3. Weskamp, G. et al. (2010). Pathological neovascularization is
reduced by inactivation of ADAM17 in endothelial cells but not in
pericytes. Circ. Res. 106:932-940.
4. Alfano, R.W. et al. (2009). Matrix metalloproteinase-activated
anthrax lethal toxin inhibits endothelial invasion and neovasculature formation during in vitro morphogenesis. Mol. Cancer Res.
7:452-461.
5. Nogueras, S. et al. (2008). Coupling of endothelial injury and
repair. An analysis using an in vivo experimental model. Am. J.
Physiol. Heart Circ. Physiol. 294:H708-H713.
Product Name
Endothelial Tube Formation Assay (In Vitro Angiogenesis)
HUVEC Tube Formation on ECM Gel. HUVEC cells from a standard tissue culture plate were incubated on an ECM gel. After several hours tube formation can be visualized under a light microscope.
Detection
Size
Catalog Number
Light Microscopy
50 Assays
CBA-200
GFP-LC3 Expression Vectors

MAP LC3 is the most published autophagosome
marker protein. LC3 associates to the inner and outer
limiting membranes of the autophagosome. Two
forms of LC3 are visible by immunoblot: LC3I found in
the soluble fraction, and LC3II found in the membrane fraction. LC3II increases during autophagy.
Our GFP-LC3 expression vectors are available in
three formats: mammalian, lentiviral, and retroviral
expression. Each vector contains a GFP reporter
gene, and a GFP control plasmid is included at no
additional charge.
Tu, S.P. et al. (2011). IFN-gamma inhibits gastric carcinogenesis
by inducing epithelial cell autophagy and T-cell apoptosis. Cancer
Res. 71:4247-4259. (CBA-401)
pCMV-GFP-LC3 Vector Map.
Product Name
24
Size
Catalog Number
pCMV-GFP-LC3 Expression Vector
100 L
CBA-401
pSMPUW-GFP-LC3 Lentiviral Expression Vector
10 g
LTV-801
pMXs-GFP-LC3 Retroviral Expression Vector
10 g
RTV-801
Phone 1-858-271-6500
Fax 1-858-271-6514
STEM CELL RESEARCH
Induced Pluripotent Stem Cells
iPS Cell Reprogramming

Reprogramming of adult cells into induced pluripotent stem cells (iPS) has provided an important new vehicle to facilitate stem cell research. Recent studies have shown that this
may be accomplished by the introduction of key genes into somatic cells by transduction
with various viral vectors or transfection of plasmids.
Retroviral and lentiviral vectors appear to achieve among the highest levels of efficiency of
iPS cell generation. We offer an extensive collection of vectors for iPS cell reprogramming.
Retroviral Vectors and Packaging Cells for iPS Cell Generation

Our iPS retroviral vectors are constructed from the pMXs vector backbone developed by Dr. Toshio Kitamura at
the University of Tokyo.* Each vector contains one of 6 factors shown to help reprogram adult fibroblasts into
iPS cells. Both human and mouse genes are available individually or in sets. Separate retroviral vectors are
available for p53 shRNA, which has been shown to potentially increase the efficiency of iPS cell generation.
Platinum Retroviral Packaging Cells provide an easy way to produce high-titer retroviruses from these stem cell
plasmids. For additional information on these cell lines please see page 61.
*Kitamura, T. et al. (2003). Exp. Hematol. 31:1007-1014.
Human iPS Vectors
Mouse iPS Vectors

Vector Backbone
Catalog Number
Oct-3/4
pMXs
RTV-705
RTV-702
Sox2
pMXs
RTV-706
pMXs
RTV-703
c-Myc
pMXs
RTV-707
Klf4
pMXs
RTV-704
Klf4
pMXs
RTV-708
NANOG
pMXs
RTV-709
NANOG
pMXs
RTV-711
Lin28
pMXs
RTV-710
Lin28
pMXs
RTV-712
Set of 4 vectors
(Oct-3-4, Sox2,
c-Myc, Klf4)
pMXs
RTV-701-C
Set of 4 vectors
(Oct-3-4, Sox2,
c-Myc, Klf4)
pMXs
RTV-705-C
Set of 6 vectors
(Oct-3-4, Sox2,
c-Myc, Klf4,
NANOG, Lin28)
pMXs
RTV-709-C
Set of 6 vectors
(Oct-3-4, Sox2,
c-Myc, Klf4,
NANOG, Lin28)
pMXs
RTV-711-C
p53 shRNA
pRetro
RTV-410
p53 shRNA
pRetro
RTV-400
Target Name
Vector Backbone
Catalog Number
Oct-3/4
pMXs
RTV-701
Sox2
pMXs
c-Myc
Target Name
Retroviral Packaging Cell Lines

Product Name
Size
Platinum-E Retroviral Packaging Cell Line, Ecotropic
>3 x 10 cells
RV-101
Platinum-A Retroviral Packaging Cell Line, Amphotropic
>3 x 106 cells
RV-102
Platinum-GP Retroviral Packaging Cell Line, Pantropic

pCMV-VSV-G Packaging Vector (for use with Platinum-GP cells)
26
Catalog Number
Phone 1-858-271-6500
>3 x 10 cells
RV-103
10 g
RV-110
Fax 1-858-271-6514
Induced Pluripotent Stem Cells
STEM CELL RESEARCH
Lentiviral Polycistronic Vector for iPS Cell Generation

Our pLentG-KOSM Lentiviral Vector provides a convenient way to generate iPS cells. The defined stem
cells factors Klf4, Oct-3/4, Sox2 and c-Myc are inframe fused into a single open reading frame (ORF)
by self-cleaving 2A peptides. The four genes are controlled by a single CMV promoter. The transcription
factor ORF is followed by IRES-GFP as a reporter to
verify viral transduction into your target cell. Efficiencies of iPS generation are typically higher compared
to transduction of four separate viruses each containing a single gene.
p53 shRNA has recently been shown to potentially
increase efficiency of iPS production. Our p53 shRNA
lentiviral vector may be transduced along with the
pLentG-KOSM vector.
Expression of Stem Cell Factors and GFP. Top: Transient expression of KOSM fusion gene in 293T cells confirmed by Western
blot. Bottom: GFP fluorescence in MEF cells 3 days after infection
with lentivirus containing KOSM fusion.
More Efficient: Up to 10-fold higher efficiency

compared to multi-virus transduction, and 500-fold
compared to non-viral methods
Reporter Convenience: Includes GFP reporter
gene to monitor lentiviral transduction
Open Reading Frame of pLentG-KOSM Lentiviral Vector.
Characterization of iPS Cell Colonies Generated from MEFs

Infected with Lentivirus Containing the KOSM Fusion. Top:
Staining of pluripotency markers in induced cell colonies at 200x
magnification. Bottom: AP staining at 100x magnification and morphology at 40x magnification in induced cell colonies.
Product Name
Size
Catalog Number
pLentG-KOSM Polycistronic Lentiviral Vector (Mouse genes)
100 L
LTV-700
pLenti-p53 shRNA (Mouse) Lentiviral Vector
100 L
LTV-451
For efficient packaging of your KOSM lentivirus, please see our ViraSafe Lentiviral
Packaging Systems on pages 49-50.
www.cellbiolabs.com
27
STEM CELL RESEARCH
Retroviral Expression Systems
Platinum Retroviral Expression Systems for Stem Cells

Retroviral vectors are useful for delivering genes of interest into a host cell where integration into the genome is
desired. However, traditional retroviral expression technologies usually result in low viral titers which make gene
expression studies difficult.
Our Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid
transfection. The Platinum Expression Systems include one of our exclusive Platinum Packaging Cell
Lines which already contain the gag and pol genes;
the Ecotropic and Amphotropic cells also contain an
envelope protein. Simply clone your gene of interest
into the vector provided and transfect into the Platinum cells. If you choose a Pantropic system, simply
co-transfect with the VSV-G plasmid provided.
The Platinum Expression Systems below are specially designed for superior expression with either ES/
EC cells or hematopoietic stem cells. For more information on our Platinum Expression Systems for a
variety of cells, please see page 60.
Ecotropic
Pantropic
Human
+++
N.S.
+++
Mouse
+++
+++
+++
Rat
+++
+++
+++
Monkey
+++
N.S.
+++
Cat
+++
N.S.
+++
Dog
+++
N.S.
+++
N.S.
+++
Bird
N.S.
N.S.
+++
Fish
N.S.
N.S.
+++
Frog
N.S.
N.S.
+++
Insect
N.S.
N.S.
+++
Mollusk
N.S.
N.S.
+++
Hamster
*Virus must be packaged with a pantropic envelope protein such as

VSVG.
Higher Viral Yields: Average titer 107 infectious units/mL with transient transfection
Versatile: 3 Packaging cell lines for use with
nearly any target host species
Optimized for Stem Cell Studies: Specially
designed expression systems for either ES/EC
cells or hematopoietic stem cells
Catalog Number
Amphotropic
N.S. = Not Suitable

Suitability of Platinum Retroviral Expression Systems by Host
Species.
Packaging Cell Line
Transfer Vector
Envelope Vector
Control Vector
VPK-303
Plat-E (Ecotropic)
pMCs-Puro
pMCs-GFP
VPK-304
Plat-A (Amphotropic)
pMCs-Puro
pMCs-GFP
VPK-305
Plat-GP (Pantropic)
pMCs-Puro
pCMV-VSV-G
pMCs-GFP
VPK-306
Plat-E (Ecotropic)
pMYs-Puro
pMYs-GFP
VPK-307
Plat-A (Amphotropic)
pMYs-Puro
pMYs-GFP
VPK-308
Plat-GP (Pantropic)
pMYs-Puro
pCMV-VSV-G
pMYs-GFP
Components of the Platinum Retroviral Expression Systems for Stem Cells.
28
Product Name
Size
Catalog Number
Platinum ES/EC Retroviral Expression System, Ecotropic
1 kit
VPK-303
Platinum ES/EC Retroviral Expression System, Amphotropic
1 kit
VPK-304
Platinum ES/EC Retroviral Expression System, Pantropic
1 kit
VPK-305
Platinum HSC Retroviral Expression System, Ecotropic
1 kit
VPK-306
Platinum HSC Retroviral Expression System, Amphotropic
1 kit
VPK-307
Platinum HSC Retroviral Expression System, Pantropic
1 kit
VPK-308
Phone 1-858-271-6500
Fax 1-858-271-6514
Feeder Cells
STEM CELL RESEARCH
Stem Cell Feeders

Leukemia inhibitory factor (LIF) is useful for maintaining the undifferentiated state of
mouse embryonic stem (mES) cells. However, LIF does not have the same effect on human embryonic stem (hES) cells. Therefore, hES cells require the use of feeder cells for
both derivation and maintenance. We offer a variety of feeder cells for stem cell culture.
All feeder cells must be mitotically inactivated prior to use.
SNL 76/7 Passage-Independent Feeder Cells for iPS Culture

The SNL 76/7 is an immortalized cell line derived
from mouse fibroblast STO cells which have been
transformed with murine LIF and neomycin resistance
genes.
Superior Culture: Transformed with LIF gene
for better maintenance of undifferentiated state
Versatile: Useful for culture of human and
mouse iPS cells and as a feeder for ES cells
Passage-Independent: Immortalized cell line

1. Osakada, F. et al (2009). In vitro differentiation of retinal cells
from human pluripotent stem cells by small-molecule induction.
J. Cell Sci. 132:3169-3179.
2. Liu, Y. et al. (2009). Zeb1 represses Mitf and regulates pigment
synthesis, cell proliferation, and epithelial morphology. Invest.
Ophthalmol. Vis. Sci. 50:5080-5088.
3. Tsubooka, N. et al. (2009). Roles of Sall4 in the generation of
pluripotent stem cells from blastocytes and fibroblasts. Genes
Cells 14:683-694.
4. Lan, Z-J. et al. (2009). Extra-germ cell expression of mouse
nuclear receptor subfamily 6, group A, member 1 (NR6A1).
Biol. Reprod. 80:905-912.
Product Name
Size
6
3 x 10 cells
SNL Feeder Cells
Catalog Number
CBA-316
JK1 Passage-Independent Feeder Cells

JK1 is an immortalized CD34+ stromal cell line that
supports long-term proliferation of stem cells. It has
been shown to maintain capacity for stem cell renewal even after serial passaging for over one year.
JK1 may be used for culture of a variety of cell types
including pluripotent ES cells, germ-line derived stem
cells such as SPCs and MASCs, and primordial germ
cell-derived EG cells.
Product Name
JK1 Cells Support Maintenance

of mES Cells. Immunofluorescence staining of germ cell nuclear antigen (GCNA). Antibody
staining is green; nuclear counterstain is blue.
Size
6
1 x 10 cells
JK1 Feeder Cells
Catalog Number
CBA-315
MEF Feeder Cells

Our murine embryonic fibroblast (MEF) feeder cells are useful for the maintenance of human or mouse ES cells
in their undifferentiated state. Cells must be mitotically inactivated prior to use.
Product Name
Size
CBA-310
CBA-313
5 x 10 cells
MEF Feeder Cells
5 x 10 cells
MEF Feeder Cells, Hygromycin-resistant
Catalog Number
MEF Feeder Cells, Neomycin-resistant
5 x 10 cells
CBA-311
MEF Feeder Cells, Puromycin-resistant
5 x 106 cells
CBA-312
www.cellbiolabs.com
29
STEM CELL RESEARCH
CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay

Hematopoietic stem cells (HSCs), when cultured in a
suitable semisolid matrix such as methylcellulose
supplemented with cytokines & nutrients, proliferate
to form discrete cell clusters or colonies. Such HSCs
or hematopoietic progenitors are known as colonyforming cells (CFCs).
In classic CFC assays, cells are cultured in a 35mm
dish for 14-21 days so the colonies can reach a certain size for manual counting, which can be tedious
and subjective.
The CytoSelect 96-Well Hematopoietic Colony
Forming Cell Assay provides a high-throughput
method to quantify CFCs in just 7-10 days with no
manual cell counting required. Cells are lysed, solubilized, and quantified using a fluorescent dye included in the kit. Alternatively, cells may be recovered for further culture and analysis.
Fast Results: 7-10 days vs. 2-3 weeks
counting
Easier Reagent Handling: Methylcellulose media
can be handled using a pipet instead of a syringe
Assay Principle for the CytoSelect 96-Well Hematopoietic
Colony Forming Cell Assay.
HSC Colony Formation. Human bone marrow derived CD34+ Hematopoietic Progenitor Cells were seeded at 3000 cells/well and cultured
for 7-10 days in the absence or presence of growth factors/cytokines. Colonies were quantified according to the assay protocol. A: After 7
days without cytokines. B: After 7 days in presence of cytokines. C: After 10 days in presence of cytokines (hemoglobin visible).
Product Name
Detection
CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay
30
Phone 1-858-271-6500
Size
Catalog Number
96 Assays
CBA-320
5 x 96 Assays
CBA-320-5
Fluorometric
Fax 1-858-271-6514
STEM CELL RESEARCH
StemTAG 96-Well Stem Cell Colony Formation Assay

Our StemTAG 96-Well Stem Cell Colony Formation
Assay provides a high-throughput method to quantify
ES cells in just 7-10 days with no manual cell counting required.
After colonies are formed, stem cells may be analyzed in 3 ways:
Fast Results: 7-10 days vs. 2-3 weeks using conventional methods
Versatile: Quantify cells using fluorescent dye,
measure alkaline phosphatase activity, or recover
cells for further analysis
Plate Reader Convenience: No manual cell
counting required
1. Lyse cells, then quantify using a fluorescent dye

included in the kit.
2. Lyse cells, then measure alkaline phosphate activity using reagents provided.
3. Recover colonies for further culture and analysis.
This assay may be of particular interest for the study
of tumor stem cells.
StemTAG Stem Cell Colony Formation Assay Principle.

Product Name
Anchorage-Independent Growth of Mouse ES-D3 Cells.

Top: Phase Contrast. Bottom: Alkaline Phosphatase Staining.
Detection
StemTAG 96-Well Stem Cell Colony Formation Assay
Size
Catalog Number
96 Assays
CBA-325
5 x 96 Assays
CBA-325-5
Fluorometric
www.cellbiolabs.com
31
STEM CELL RESEARCH
Alk Phos Assays, Primers, RNA/Protein
StemTAG Alkaline Phosphatase Kits

The StemTAG Alkaline Phosphatase Staining and
Activity Assay Kits monitor AP activity via both immunocytochemistry staining and a colorimetric activity
assay. The staining and activity assay kits are also
sold separately.
StemTAG Alkaline Phosphatase Staining Kit. Murine embryonic stem cells (ES-D3) were maintained in an undifferentiated
state with LIF. To induce differentiation, LIF was withdrawn over
several days. Various differentiation events were observed: cells
became flattened and enlarged with reduced proliferation. On day
5, cells were stained according to the assay protocol.
Product Name
Fast Results: Staining and Activity Assay protocols each take less than 1 hour
Versatile: Useful for human ES, EG and EC cells,
as well as mouse ES and EG cells

1. Lee, J. et al. (2010). Ultraviolet A regulates adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells
via up-regulation of kruppel-like factor 2. J. Biol. Chem.
285:32647-32656. (CBA-300)
2. Izadyar, F. et al. (2008). Generation of multipotent cell lines from
a distinct population of male germ line stem cells. Reproduction
135:771-784. (CBA-300)
3. Kim, Y. et al. (2009). Cyclin-dependent kinase 2-associating
protein 1 commits murine embryonic stem cell differentiation
through retinoblastoma protein regulation. J. Biol. Chem.
284:23405-23414. (CBA-301)
4. Yue, Y. et al. (2008). A single intravenous injection of adenoassociated virus serotype-9 leads to whole body skeletal muscle
transduction in dogs. Mol. Ther. 16(12):1944-1952. (CBA-301)
5. Ghosh, A. et al (2007). Efficient whole-body transduction with
trans-splicing AAV vectors. Mol. Ther. 15(4):750-755. (CBA-301)
Detection
Size
Catalog Number
ICC & Colorimetric
2 x 100 Assays
CBA-302
ICC & Fluorometric
2 x 100 Assays
CBA-308
Colorimetric
100 Assays
CBA-301
Fluorometric
100 Assays
CBA-307
ICC
100 Assays
CBA-300
StemTAG Alkaline Phosphatase Staining and Activity Assay Kit
StemTAG Alkaline Phosphatase Activity Assay Kit

StemTAG Alkaline Phosphatase Staining Kit
StemTAG PCR Primer Set for Stem Cell Characterization

Pluripotent stem cells can differentiate into cells derived from all three embryonic germ layers: endoderm,
mesoderm and ectoderm. Our StemTAG PCR Primer Set provides an efficient system for monitoring ES cell
differentiation/undifferentiation. Seven primer sets are included: primers for two widely studied stem cell markers (Oct-4 and NANOG), one marker for each embryonic germ layer (AFP/Endoderm, Flk-1/Mesoderm and
NCAM/Ectoderm), and two controls (GAPDH and -Actin). Primers are suitable for either end-point or real-time
(quantitative) PCR.
Product Name
StemTAG PCR Primer Set for Stem Cell Characterization
Size
Catalog Number
50 Reactions
CBA-303
Total RNA and Protein from Murine ES-D3 Cell Line
32
Product Name
Size
Catalog Number
Total RNAMurine Embryonic Stem Cell Line D3
50 g
CBA-304
Total ProteinMurine Embryonic Stem Cell Line D3
500 g
CBA-305
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION
Viral Vector Overview
Recombinant Viral Gene Delivery

Recombinant viral vectors provide a powerful means of delivering a gene into a target cell.
There are many viral vectors available, and there are pros and cons to each. Use the following table to select the best viral vector for your research.
Comparison of Viral Vectors for Gene Delivery

Adeno-Associated
Virus (AAV)
p. 35-40
Adenovirus
p. 41-48
Lentivirus
(HIV-1, FIV, SIV)
p. 49-55
Retrovirus
(MMLV)
p. 56-64
Transient
or Stable
Transient
Transient
or Stable
Stable
Will Infect Dividing Cells
Yes
Yes
Yes
Yes
Will Infect Non-Dividing Cells
Yes
Yes
Yes
No
Integrates into Target Cell Genome
No*
No
Yes
Yes
Immune Response in Target Cells
Very Low
High
Low
Moderate
Relative Viral Titer
XXX
XXXX
XXX
XX
Relative Transduction Efficiency
XXX
XXXX
XXX
XX
Gene Expression
*Native AAV can integrate, but recombinant AAV rarely does.
Typical Workflow for Viral Gene Delivery
Clone Gene
of Interest
Viral
Expression
Plasmid
Package
Virus
Packaging
Plasmids
and Cells
Measure
Titer
Viral
Quantitation
Kit
Concentrate
& Purify
Purification &
Concentration
Kit
Infect
Target Cell
Viral
Transduction
Reagents
Cell Biolabs offers kits and reagents for every step in your workflow.
34
Phone 1-858-271-6500
Fax 1-858-271-6514
AAV Expression
VIRAL EXPRESSION
Adeno-Associated Virus Kits & Reagents

Adeno-associated virus (AAV) is less immunogenic than adenovirus or retrovirus. We offer
a comprehensive line of AAV kits and reagents to ensure you get the best expression from
your AAV expression studies:
Helper Free Expression Systems
Premade AAV Controls
Helper Free Packaging Systems
Purification Kits
Expression & Control Vectors
Quantitation / Titer Kit
Viral Packaging Cell Line
Transduction Kits
AAV Helper Free Systems

Production of recombinant AAV requires certain
genes from the adenovirus genome, which means
that an adenovirus usually needs to be present. The
AAV Helper Free System eliminates the need for a
helper adenovirus. Most of the required adenoviral
genes (E2A, E4 and VA RNA) are provided in a
pHelper plasmid, while the required E1 gene is provided by the 293 packaging cells.
Safer: pHelper plasmid eliminates the need for a

helper virus
Flexible: Packaging vectors and expression vectors available separately or as one complete system
Control Vectors: GFP, Cre or LacZ
Transduction of AAV-GFP using the AAV Helper Free System.

GFP expression in 293AD cells 48 hours after transduction (20X).
AAV Helper Free Systems are available

for a variety of serotypes and kit formats.
Ordering information for AAV Helper
Free Expression Systems, Packaging
Systems, Expression Vectors, and
Control Vectors may be found on the
following pages.
Gene Delivery using the AAV Helper Free System.
www.cellbiolabs.com
35
VIRAL EXPRESSION
AAV Expression
AAV Helper Free Complete Expression Systems

AAV Helper Free Complete Expression Systems contain everything you need to produce high-titer recombinant adeno-associated virus:
AAV Expression Vector
pHelper Plasmid
pRep-Cap Plasmid (serotype specific)
GFP Control Vector
AAV Helper Free Expression Systems are available

for the following serotypes:
Native serotypes 1-6
AAV-DJ, engineered by DNA family shuffling to
form a hybrid capsid from 8 different native serotypes; provides significantly higher infectivity
rates in vitro (see table below)
AAV-DJ/8, a mutant of AAV-DJ that exhibits increased uptake in brain and other tissues in vivo,
similar to serotypes 8 and 9
Cell Line
Cell or Tissue Source
AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6
AAV-8 AAV-9
AAVDJ
AAVDJ/8
Huh-7
Hu Liver
13
100
2.5
0.0
0.1
10
0.7
0.0
500
0.2
HEK293
Hu Kidney
25
100
2.5
0.1
0.1
0.7
0.1
500
0.3
HeLa
Hu Cervix
100
2.0
0.1
3.7
1.0
0.2
0.1
667
0.2
HepG2
Hu Liver
100
16.7
0.3
1.7
0.3
ND
1250
0.5
Hep1A
Ms Liver
20
100
0.2
911
Hu Retina
17
100
11.1
1.0
0.1
1.0
0.2
0.0
400
0.1
0.2
0.1
17
0.1
ND
500
0.0
CHO
Hm Ovary
100
100
14.3
1.4
333
50
10.0
1.0
25000
5.0
COS
Si Kidney
33
100
33
3.3
5.0
14
2.0
0.5
500
0.3
MeWo
Hu Skin
10
100
20
0.3
6.7
10
1.0
0.2
2857
1.0
NIH3T3
Ms Fibroblasts
A549
Hu Lung
10
100
2.9
2.9
0.3
10
0.3
ND
500
0.1
14
100
20
ND
0.5
10
0.5
0.1
1000
0.1
HT1180
Hu Fibroblasts
20
100
10.0
0.1
0.3
33
0.5
0.1
333
0.2
Monocytes
Hu Primary Monocytes
1111
100
ND
ND
125
1429
ND
ND
100
ND
Immature DC
Hu Monocyte-derived DC
2500
100
ND
ND
222
2857
ND
ND
200
ND
Mature DC
Hu Monocyte-derived DC
2222
100
ND
ND
333
3333
ND
ND
100
ND
Relative Infectivity Rates of AAV Serotypes. Normalized to AAV-2 = 100. ND = Not determined.
36
Product Name
Size
Catalog Number
AAV-DJ Helper Free Expression System
1 kit
VPK-410-DJ
AAV-DJ Helper Free Bicistronic Expression System (Puro)
1 kit
VPK-415-DJ
AAV-DJ Helper Free Bicistronic Expression System (Neo)
1 kit
VPK-416-DJ
AAV-DJ Helper Free Bicistronic Expression System (Hygro)
1 kit
VPK-417-DJ
AAV-DJ Helper Free Bicistronic Expression System (GFP)
1 kit
VPK-418-DJ
AAV-DJ Helper Free Bicistronic Expression System (Blasticidin)
1 kit
VPK-419-DJ
AAV-DJ/8 Helper Free Expression System
1 kit
VPK-410-DJ-8
AAV-DJ/8 Helper Free Bicistronic Expression System (Puro)
1 kit
VPK-415-DJ-8
AAV-DJ/8 Helper Free Bicistronic Expression System (Neo)
1 kit
VPK-416-DJ-8
AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)
1 kit
VPK-417-DJ-8
AAV-DJ/8 Helper Free Bicistronic Expression System (GFP)
1 kit
VPK-418-DJ-8
AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin)
1 kit
VPK-419-DJ-8
Phone 1-858-271-6500
Fax 1-858-271-6514
AAV Expression
VIRAL EXPRESSION
AAV Helper Free Complete Expression Systems (continued)

Product Name
Size
Catalog Number
AAV-1 Helper Free Expression System
1 kit
VPK-410-SER1
AAV-1 Helper Free Bicistronic Expression System (Puro)
1 kit
VPK-415-SER1
AAV-1 Helper Free Bicistronic Expression System (Neo)
1 kit
VPK-416-SER1
AAV-1 Helper Free Bicistronic Expression System (Hygro)
1 kit
VPK-417-SER1
AAV-1 Helper Free Bicistronic Expression System (GFP)
1 kit
VPK-418-SER1
AAV-1 Helper Free Bicistronic Expression System (Blasticidin)
1 kit
VPK-419-SER1
1 kit
VPK-410-SER2
1 kit
VPK-415-SER2
1 kit
VPK-416-SER2
1 kit
VPK-417-SER2
1 kit
VPK-418-SER2
1 kit
VPK-419-SER2
1 kit
VPK-410-SER3
1 kit
VPK-415-SER3
1 kit
VPK-416-SER3
1 kit
VPK-417-SER3
1 kit
VPK-418-SER3
1 kit
VPK-419-SER3
1 kit
VPK-410-SER4
1 kit
VPK-415-SER4
1 kit
VPK-416-SER4
1 kit
VPK-417-SER4
1 kit
VPK-418-SER4
1 kit
VPK-419-SER4
1 kit
VPK-410-SER5
1 kit
VPK-415-SER5
1 kit
VPK-416-SER5
1 kit
VPK-417-SER5
1 kit
VPK-418-SER5
1 kit
VPK-419-SER5
1 kit
VPK-410-SER6
1 kit
VPK-415-SER6
1 kit
VPK-416-SER6
1 kit
VPK-417-SER6
1 kit
VPK-418-SER6
1 kit
VPK-419-SER6
www.cellbiolabs.com
37
VIRAL EXPRESSION
AAV Expression
AAV Helper Free Packaging Systems

AAV Helper Free Packaging Systems contain everything found in the Complete Expression Systems, with the
exception of the AAV expression vector. This is an ideal choice if you already have an AAV construct containing
your gene of interest. All plasmids are provided individually, not as a packaging mixture.
Product Name
Size
Catalog Number
AAV-DJ Helper Free Packaging System
1 kit
VPK-400-DJ
AAV-DJ/8 Helper Free Packaging System
1 kit
VPK-400-DJ-8
AAV-1 Helper Free Packaging System
1 kit
VPK-401
1 kit
VPK-402
1 kit
VPK-403
1 kit
VPK-404
1 kit
VPK-405
1 kit
VPK-406
Product Name
Size
Catalog Number
pAAV-MCS Expression Vector
10 g
VPK-410
pAAV-MCS Promoterless Expression Vector
10 g
VPK-411
pAAV-IRES-Puro Expression Vector
10 g
VPK-415
pAAV-IRES-Neo Expression Vector
10 g
VPK-416
pAAV-IRES-Hygro Expression Vector
10 g
VPK-417
pAAV-IRES-GFP Expression Vector
10 g
VPK-418
pAAV-IRES-Bsd Expression Vector
10 g
VPK-419
Product Name
Size
Catalog Number
pAAV-GFP Control Vector
10 g
AAV-400
pAAV-Cre Control Vector
10 g
AAV-401
pAAV-LacZ Control Vector
10 g
AAV-402
Product Name
Size
Catalog Number
AAV1-GFP Control Virus
50 L
AAV-301
50 L
AAV-302
50 L
AAV-303
50 L
AAV-305
50 L
AAV-306
AAV Helper Free Expression Vectors
AAV Helper Free Control Plasmids
AAV Premade Control Viruses

All AAV-GFP premade viruses are provided at a concentration of 1 x 1012 GC/mL.
38
Phone 1-858-271-6500
Fax 1-858-271-6514
AAV Expression
VIRAL EXPRESSION
AAV Packaging Cell Line

Our 293AAV cell line is selected from the parental 293 cell line for larger surface area, flattened morphology,
and firmer attachment to culture plates, resulting in production of higher yields of AAV.
Product Name
Size
Catalog Number
1 x 10 cells
293AAV Cell Line
AAV-100
ViraBind AAV Purification Kits

Purification of AAV via ultracentrifugation can be tedious and time-consuming, and may result in low yields.
ViraBind AAV Purification Kits use a one-step proprietary matrix followed by further purification and concentration using a centrifugal concentrator. The result is a higher AAV yield with high purity in a fraction of the time.
Kits are suitable for AAV-2 or AAV-DJ; they will not work with other AAV serotypes.
High Purity: No contamination bands as seen
on SDS gel
Fast Results: Obtain purified virus in about 3
hours
High Yields: Recovery rate >60%

Uchida, S. et al. (2010). Early life stress enhances behavioral
vulnerability to stress through the activation of REST4-mediated
gene transcription in the medial prefrontal cortex of rodents. J.
Neurosci. 30:15007-15018. (VPK-140)
Purification Procedure for the ViraBind AAV Purification Kit.
Electrophoretic Profile of Purified AAV2-GFP.
Product Name
Capacity/Prep
Size
Catalog Number
ViraBind AAV Purification Kit
2 x 10cm dishes
10 Preps
VPK-140
2 Preps
VPK-141
ViraBind AAV Purification Mega Kit
10 x 15cm dishes
10 Preps
VPK-141-5
www.cellbiolabs.com
39
VIRAL EXPRESSION
AAV Expression
QuickTiter AAV Quantitation Kit

Traditional AAV Quantitation by dot blot can be tedious, time consuming, and suffer from high inter-assay
variability. Our QuickTiter AAV Quantitation Kit
uses a proprietary technology to quantify AAV nucleic
acid content of unpurified AAV-2 or AAV-DJ, or from
purified AAV of any serotype.
Fast Results: Obtain purified virus in less than 2

hours
High Sensitivity: Limit of detection 1 x 109 GC/mL
from unpurified supernatant or 5 x 1010 GC/mL from
purified AAV
20
200
175
15
150
RFU
RFU
125
100
10
75
50
25
0
0
25
50
75
100
125
150
10
AAV DNA STD (ng)
AAV DNA STD (ng)
AAV-2 DNA Standard Curve. The QuickTiter AAV-2 DNA Standard was diluted as described in the assay protocol. Fluorescence was
measured on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.
Product Name
QuickTiter AAV Quantitaiton Kit
Capacity/Prep
Size
Catalog Number
Fluorometric
20 Assays
VPK-145
ViraDuctin AAV Transduction Reagent

Successful gene expression studies using AAV depend on high transduction efficiencies into host cells. Infection
rates appear to be highest in S-phase cells, which can account for a very small fraction of a cell population.
Our ViraDuctin AAV Transduction Reagent can
significantly increase the transduction efficiency of
AAV vectors in both dividing and non-dividing cells.
Increases are greatest in non-dividing cells, but even
cells in S-phase show a noticeable increase in transduction efficiencies.
Higher Efficiencies: Significantly increase rate of

infection of host cells
Low Toxicity: No noticeable effect on cell viability
Universal: Suitable for use with both dividing and
non-dividing cells
Product Name
Size*
Catalog Number
10 Transductions
AAV-200
50 Transductions
AAV-201
ViraDuctin AAV Transduction Reagent

*Number of transductions performed in 35mm culture dishes. May be modified for use in culture plates or larger dishes. See product insert.
40
Phone 1-858-271-6500
Fax 1-858-271-6514
Adenoviral Expression
VIRAL EXPRESSION
Adenoviral Expression Kits & Reagents

Recombinant adenoviruses are excellent tools for introducing genetic material into host
cells, since they can infect a variety of mammalian cell types with high efficiency. They remain epichromosal upon infection, so they are only suitable for transient gene expression.
We offer a complete workflow solution to your adenoviral expression studies:
Viral Expression Systems

Viral Packaging Cell Line
Premade Recombinant Adenoviruses
Purification Kits
Quantitation / Titer Kits
Transduction Reagents
RAPAd Adenoviral Expression Systems

Adenoviruses are useful for delivering genes into a
wide variety of cell types, but traditional recombination methods take several months and require tedious
plaque purification. Recent technologies have shortened the time required but still produce relatively high
levels of replication-competent plaques.
RAPAd Adenoviral Expression Systems produce
recombinant adenovirus in about 2 weeks with a
substantial reduction in wild-type adenovirus. The
systems use a backbone vector from which the 5
ITR, packaging signal and E1 sequences have been
removed. Additionally, serial amplification of the recombinant adenovirus does not increase the level of
replication-competent adenovirus.
Virtually No Wild-Type Virus: Backbone vector
engineered to produce <300 wild-type plaques per
109 particles, compared with 104-106 WT plaques
per 109 VP with most other methods
Faster Production: Virus generated in 2 weeks
compared to a few months with traditional methods
5 Complete Systems: Choose CMV or RSV as
your promoter for gene expression, EF-1 for miRNA
expression, or clone your own promoter along with
your gene of interest for maximum versatility
Kothari, H. et al. (2010). Cystine 186-cystine 209 disulfide bond is
not essential for the procoagulant activity of tissue factor or for its
de-encryption. Blood 115:4273-4283. (VPK-252)
Product Name
Adenovirus Production using the RAPAd

Adenoviral Expression System.
Promoter
Size
Catalog Number
None*
1 Kit
VPK-250
RAPAd RSV Adenoviral Expression System
RSV
1 Kit
VPK-251
RAPAd CMV Adenoviral Expression System
CMV
1 Kit
VPK-252
RAPAd Bicistronic Adenoviral Expression System (GFP)
CMV
1 Kit
VPK-254
RAPAd miRNA Adenoviral Expression System
EF-1
1 Kit
VPK-253
RAPAd Universal Adenoviral Expression System
*Allows you the flexibility to clone your own promoter along with your gene of interest.
www.cellbiolabs.com
41
VIRAL EXPRESSION
293AD Cell Line for Adenoviral Packaging and Amplification

The 293AD cell line is derived from the parental 293
cell line, but has been specifically selected for adenovirus applications and offers advantages over conventional 293 cells: flattened morphology, firm attachment to culture plates, and a larger surface area for
superior transfection and greater viral yields.

1. Kothari, H. et al. (2010). Cystine 186-cystine 209 disulfide bond
is not essential for the procoagulant activity of tissue factor or
for its de-encryption. Blood 115:4273-4283. (AD-100)
2. Fang, S. et al (2007). Coordinated recruitment of histone methyltransferase G9a and other chromatin modifying enzymes in
SHP-mediated regulation of hepatic bile acid metabolism. Mol.
Cell Biol. 27:1407-1424. (AD-100)
Product Name
Size
Catalog Number
AD-100
1 x 10 Cells
293AD Cell Line

Angiogenesis
Dont have time to make your own adenovirus? Are

you studying the expression of multiple genes?
Rely on our premade recombinant adenoviruses
that already contain a gene of interest.
All of Cell Biolabs premade recombinant adenoviruses contain 5 x 109 viral particles per vial. They
are provided as 50 l aliquots at a concentration of
1 x 1011 viral particles/mL in TBS with 10% glycerol.
Controls and Reporter Genes

1. Matsushita, T. et al. (2009). FGFR3 promotes synchondosis
closure and fusion of ossification centers through the MAPK
pathway. Hum. Mol. Genet. 18:227-240. (ADV-001)
2. Ackerman, W. et al (2008). Nuclear Factor-kappa B regulates
inducible prostaglandin E synthase expression in human amnion mesenchymal cells. Biol. Reprod. 78:68-76. (ADV-002)
3. Takeda, R. et al (2008). Calcineurin is critical for sodiuminduced neointimal formation in normotensive and hypertensive
rats. Am. J. Physiol. Heart Circ. Physiol. 294:H2871-H2878.
(ADV-002)
4. Jones, S.W. et al. (2009). Mitogen-activated protein kinaseactivated protein kinase (MK2) modulates key biological pathways associated with OA disease pathology. Osteoarthritis and
Cartilage 17:124-131. (ADV-004)
5. Perez-Moreno, M. et al. (2008). Loss of p120 catenin and links
to mitotic alterations, inflammation, and skin cancer. PNAS
105:15399-15404. (ADV-004)
6. Kato, H. et al. (2011). Wnt/-Catenin pathway in podocytes
integrates cell adhesion, differentiation and survival. J. Biol.
Chem. 286:26003-26015. (ADV-005)
Target Name
42
Blood Vessel Formation After 3 Days. Purified Ad-Null or AdVEGF viruses were applied to a 10-day old CAM (chick chorioallanoic membrane). Results were visualized by stereomicroscope.
1. Stoletov, K. et al. (2010). Visualizing extravasation dynamics of
metastatic tumor cells. J. Cell Sci. 123:2332-2341. (ADV-101)
2. Serban, D. et al. (2008). H-ras regulates angiogenesis and
vascular permeability by activation of distinct downstream effectors. Circ. Res. 102(11):1350-1358. (ADV-101)
3. Stoletov, K. et al (2008). High resolution imaging of the dynamic tumor cell-vascular interface in transparent zebrafish.
PNAS 104:17406-17411. (ADV-101)
Target Name
Catalog Number
HIF-1
ADV-100
VEGF
ADV-101
Catalog Number
Null Control (No gene)
ADV-001
-Galactosidase
ADV-002
Cre
ADV-005
Target Name
Firefly Luciferase
ADV-008
Carbonic Anhydrase 9 (CA9)
ADV-602
GFP
ADV-004
Carcinoembryonic Antigen (CEA)
ADV-604
SEAP (Secretory Alkaline Phosphatase)
ADV-003
NY-ESO-1
ADV-601
Phone 1-858-271-6500
Cancer/Tumor Antigens
Catalog Number
Fax 1-858-271-6514
VIRAL EXPRESSION
Premade Recombinant Adenoviruses, continued

Cell Cycle & Transcription Regulation
Target Name
Catalog Number
Target Name
Catalog Number
DCC
ADV-504
p53
ADV-501
MyoD
ADV-508
p53 (Temperature Sensitive Mutant)
ADV-502
Myogenin
ADV-509
p68 RNA Helicase
ADV-505
Cytoskeleton Regulation / Small GTPase
Actin Cytoskeleton Staining. Cos-7 cells were infected with purified Ad-Ras V12 (ADV-146) at 50 MOI (multiplicity of infection).
Membrane ruffling was visualized by staining the actin cytoskeleton
with Rhodamine-coupled Phalloidin.
Target Name
Catalog Number

1. Black, S.A. et al (2008). TGF1 stimulates connective tissue
growth factor (CCN2/CTGF) expression in human gingival
fibroblasts through a RhoA-independent, Rac1/Cdc42dependent mechanism: statins with forskolin block TGF1induced CCN2/CTGF expression. J. Biol. Chem. 283:1083510847. (ADV-145, ADV-150, ADV-153, ADV-156)
2. Thomas, M.A. et al. (2009). E4orf1 limits the oncolytic potential
of the E1B-55K-deleted adenovirus. J. Virol. 83:2406-2416.
(ADV-150)
3. Rendon, B. et al. (2007). Regulation of human lung adenocarcinoma cell migration and invasion by MIF. J. Biol. Chem.
282:29910-29918. (ADV-150)
4. Yu, W.-M. et al. (2009). Laminin is required for Schwann cell
morphogenesis. J. Cell Sci. 122:929-936. (ADV-150, ADV-153,
ADV-154)
5. Cheng, Z.-J. et al. (2010). Co-regulation of caveolar and Cdc42
-dependent fluid phase endocytosis by phosphocaveolin-1. J.
Biol. Chem. 285:15119-15125. (ADV-153)
105:15399-15404. (ADV-156)
7. Vaught, D. et al. (2009). Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol.
Biol. Cell 20:2572-2581. (ADV-157)
8. Brantley-Sieders, D. et al. (2008). The receptor tyrosine kinase
EphA2 promotes mammary adenocarcinoma tumorigenesis
and metastatic progression in mice by amplifying ErbB2 signal.
J. Clin. Invest. 118:64-78. (ADV-157)
9. Fang, W.B. et al. (2008). Overexpression of EPHA2 receptor
destabilizes adherens junctions via a RhoA-dependent mechanism. J. Cell Sci. 121:358-368. (ADV-157)
10.Moldobaeva, A. et al. (2008). MIP-2 causes differential activation of RhoA in mouse aortic versus pulmonary artery endothelial cells. Microvascular Res. 75:53-58. (ADV-157)
Cdc42
ADV-152
Cdc42 L61 (Constitutively Active)
ADV-154
Cdc42 N17 (Dominant Negative)
ADV-153
PAK1
ADV-202
PAK1 (H83L, H86L)
ADV-203
PAK1 (H83L, H86L, K299R)
ADV-205
PAK1 (K299R)
ADV-207
Target Name
PAK1 (L107E, T423E)
ADV-206
Ras N17 (Dominant Negative)
ADV-145
PAK1 (T423E)
ADV-204
Ras V12 (Constitutively Active)
ADV-146
PAK1 (Kinase Domain)
ADV-209
Ras V12C40
ADV-148
PAK1 (Regulatory Domain)
ADV-208
Ras V12S35
ADV-147
Rac1
ADV-149
Rho L63 (Constitutively Active)
ADV-157
Rac1 L61 (Constitutively Active)
ADV-151
Rho N19 (Dominant Negative)
ADV-156
Rac1 N17 (Dominant Negative)
ADV-150
SDF-1
ADV-210
www.cellbiolabs.com
Catalog Number
43
VIRAL EXPRESSION

MAP Kinase Signaling
Immunoblot of Various
Cell Signaling Targets.
HUVEC cells were infected with purified AdNull (ADV-001), Ad-Ras
V12 (ADV-146), Ad-Ras
V12S35 (ADV-147), AdRas V12C40 (ADV-148),
Ad-MEK1 (ADV-119),
and Ad-Rac1 L61 (ADV151) at 10 MOI
(multiplicity of infection).
Cell lysates were analyzed for gene expression and ERK activation.
See following page for recent citations

using these adenoviruses
Target Name
44
Catalog Number
Target Name
Catalog Number
MKK3
ADV-120
MKK3 (Dominant Negative)
ADV-121
MKK3 (Constitutively Active)
ADV-122
ADV-160
ADV-161
MKK6
ADV-123
ADV-124
ADV-125
MKK7
ADV-126
ADV-127
ADV-128
myr-Rac1
ADV-163
Cdc42
ADV-152
p38
ADV-104
ADV-154
p38 (Dominant Negative)
ADV-105
ADV-153
p38
ADV-106
ERK2
ADV-112
ADV-107
ERK2 (Dominant Negative)
ADV-113
p38
ADV-108
ERK5 (BMK1)
ADV-116
ADV-109
ERK5 (Dominant Negative)
ADV-117
ADV-111
Interferon-
ADV-103
PRAK (Dominant Negative)
ADV-141
Interleukin-2
ADV-102
Rac1
ADV-149
JNK1
ADV-114
ADV-151
JNK1 (Dominant Negative)
ADV-115
ADV-150
MAPKAPK2
ADV-137
Raf1
ADV-132
MAPKAPK2 (Dominant Negative)
ADV-138
Raf1 (Dominant Negative)
ADV-133
MAPKAPK2 (Constitutively Active)
ADV-139
Raf1 (Constitutively Active)
ADV-134
MEK1 (Dominant Negative)
ADV-118
ADV-145
MEK1 (Constitutively Active)
ADV-119
ADV-146
MEK5
ADV-129
ADV-157
MEK5 (Dominant Negative)
ADV-130
ADV-156
MEK5 (Constitutively Active)
ADV-131
SOK
ADV-142
MEKK1
ADV-135
SOK (Dominant Negative)
ADV-143
MEKK1 (Dominant Negative)
ADV-136
SOK (Constitutively Active)
ADV-144
MEKK3
ADV-162
Tac-Rac1 (Membrane Targeting)
ADV-164
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION

MAP Kinase Signaling, continued
1. Jones, S.W. et al. (2009). Mitogen-activated protein kinaseactivated protein kinase (MK2) modulates key biological pathways associated with OA disease pathology. Osteoarthritis and
Cartilage 17:124-131. (ADV-105)
2. Kim, J.M. et al. (2008). Inhibition of apoptosis in Bacteroids
fragilis enterotoxin-stimulated intestinal epithelial cells through
the induction of c-IAP-2. Eur. J. Immunol. 38(8):2190-2199.
(ADV-105)
3. Naimi, M. et al (2007). Nuclear forkhead box O1 controls and
integrates key signaling pathways in hepatocytes. Endocrinology 148:2424-2434. (ADV-105)
4. Lee, J.Y. et al. (2007). Effects of transcription factor activator
protein-1 on interleukin-8 expression and enteritis in response
to Clostridium difficile toxin A. J. Mol. Med. 85:1393-1404.
(ADV-105, ADV-115)
5. Monick, M. et al (2008). Constitutive ERK MAPK activity regulates macrophage ATP production and mitochondrial integrity.
J. Immunol. 180:7485-7496. (ADV-112, ADV-113, ADV-118,
ADV-119)
6. Samuel, I. et al. (2008). Enteral exclusion increases MAP
kinase activation and cytokine production in a model of gallstone pancreatitis. Pancreatology 8(1):6-14. (ADV-113)
7. Wang, X. et al (2007). Human immunodeficiency virus protease
inhibitor ritonavir inhibits cholesterol efflux from human macrophage-derived foam cells. Am. J. of Pathology 171:304-314.
(ADV-113)
8. Black, S. et al (2007). Tissue-specific mechanisms for CCN2/
CTGF persistence in fibrotic gingiva. J. Biol. Chem. 282
(21):15416-15429. (ADV-115)
9. Vadysirisack, D. et al (2007). MEK signaling modulates sodium
iodide symporter at multiple levels and in a paradoxical manner. Endocrine-Related Cancer 14:421-432. (ADV-118)
10. Matsushita, T. et al. (2009). FGFR3 promotes synchondosis
closure and fusion of ossification centers through the MAPK
pathway. Hum. Mol. Genet. 18:227-240. (ADV-119)
11. Yoon, C-H. et al. (2009). Activation of p38 mitogen-activated
protein kinase is required for death receptor-independent caspase-8 activation and cell death in response to sphingosine.
Mol. Cancer Res. 7(3):361-370. (ADV-119)
12. Tan, S.H. et al. (2009). Regulation of cell proliferation and migration by TAK1 via transcriptional control of von Hippel-Lindau
tumor suppressor. J. Biol. Chem. 284:18047-18058. (ADV-128)
13. Black, S.A. et al. (2008). TGF1 stimulates connective tissue
morphogenesis. J. Cell Sci. 122:929-936. (ADV-150)
(ADV-150)
J. Clin. Invest. 118:64-78. (ADV-157)
17. Fang, W.B. et al (2008). Overexpression of EPHA2 receptor
18. Taniguchi, C. et al (2007). The p85a regulatory subunit of
phosphoinositide 3-kinase potentiates c-Jun N-terminal kinasemediated insulin resistance. Mol. Cell Biol. 27:2830-2840.
(ADV-161)
NFB Signaling
1. Johnston, R.K. et al. (2009). 3-integrin mediated ubiquitination
activates survival signaling during myocardial hypertrophy.
FASEB J. 23(8):2759-2771. (ADV-302)
2. Martin, A.P. et al. (2008). Lapatinib resistance in HCT116 cells
is mediated by elevated MCL-1 expression and decreased BAK
activation and not by ERBB receptor kinase mutation. Mol.
Pharmacol. 74:807-822. (ADV-302)
3. Richardson, W.M. et al. (2010). Nucleotide-binding oligomerization domain-2 inhibits toll-like receptor-4 signaling in the
intestinal epithelium. Gastroenterology 139(3):904-917. (ADV308, ADV-309)
Target Name
Catalog Number
IB-
ADV-301
IB- S32A (Dominant Negative)
ADV-302
IKK-
ADV-305
IKK- (Dominant Negative)
ADV-303
NOD2
ADV-308
NOD2 (Frame Shift Mutation)
ADV-309
Rel B
ADV-304
Tyrosine Kinases and PKCs

1. Koh, W. et al. (2009). Formation of endothelial lumens requires
a PKC-, Src-, Pak-, and Raf-kinase dependent signaling cascade downstream of Cdc42 activation. J. Cell Sci. 122:18121822. (ADV-401, ADV-405, ADV-406)
2. Lecuone, E. et al. (2009). Ubiquitination participates in the lysosomal degradation of the Na,K-ATPase in steady state conditions. Am. J. Respir. Cell Mol. Biol. 41(6):617-679. (ADV-412)
3. Briva, A. et al. (2007). High CO2 levels impair alveolar epithelial
function independent of pH. PLoS ONE 2(11):e1238. (ADV412)
Target Name
Catalog Number
CSK
ADV-405
CSK (Dominant Negative)
ADV-406
Fyn
ADV-403
Fyn (Dominant Negative)
ADV-404
PKC- (Dominant Negative)
ADV-410
ADV-411
ADV-412
shAkt1
ADV-417
shAkt2
ADV-418
Src
ADV-401
www.cellbiolabs.com
45
VIRAL EXPRESSION
ViraBind Adenovirus Purification Kits

Purification of viruses via cesium chloride (CsCl) ultracentrifugation procedures can be tedious and timeconsuming. ViraBind Adenovirus Purification Kits
use an efficient system for quick adenoviral purification with high recovery. No ultracentrifugation is required. Kits use either a spin column or syringe filter
for high purity adenovirus (see selection guide).
High Viral Yield: >90% recovery
High Quality: Provides quality of CsCl procedures,
but in much less time
Faster Results: 30 minutes (1-2 hrs for Mega kit)
User-Friendly Protocol: No gradient preparation
or ultracentrifugation steps
Relative VP (%)
100
80
60
40
20
El
ut
io
n
2n
W
as
tW
as
h
1s
gh
ro
u
Fl
ow
- th
Vi
ra
l
Su
pe
rn
a
ta
n
Purification of Recombinant Ad--Gal. Ad--Gal was purified

according to the assay protocol. Each purification fraction was
used to infect A549 cells in a 12-well plate. After 48 hr, cells were
scored using our -Galactosidase Staining Kit (p. 92).

1. Kirui, J.K. et al. (2010). Ggamma signaling promotes breast
cancer cell migration and invasion. J. Pharmacol. Exp. Ther.
333:393-403. (VPK-099)
2. Chen, F. et al. (2011). Dynamic regulation of PDX-1 and
FoxO1 expression by FoxA2 in dexamethasone-induced pancreatic -cells dysfunction. Endocrinology 152:1779-1788.
(VPK-100)
3. Prasad, S.S. et al. (2011). Enzymatic activities of the human
AGPAT isoform 3 and isoform 5: localization of AGPAT5 to
mitochondria. J. Lipid Res. 52:451-462. (VPK-100)
4. Agarwal, A.K. et al. (2010). Enyzmatic activity of the human 1acylglycerol-3-phosphate-O-acyltransferase isoform 11:
upregulated in breast and cervical cancers. J. Lipid Res.
51:2143-2152. (VPK-100)
5. Triulzi, C. et al. (2010). Antibody-dependent natural killer cellmediated cytotoxicity engendered by a kinase-inactive HER2
adenovirus-based vaccination mediates resistance to breast
tumors. Cancer Res. 70:7431-7441. (VPK-100)
6. Sen, P. et al. (2010). Zinc modulates the interaction of protein
C and activated protein C with endothelial protein C receptor. J.
Biol. Chem. 285:20410-20420. (VPK-100)
7. Sabbatini, M. E. et al. (2010). CCK activates RhoA and Rac1
differentially through G-alpha-13 and G-alpha-q in mouse pancreatic acini. Am. J. Physiol. Cell Physiol. 298:C592-C605.
(VPK-100)
8. Lam, Y.W. et al. (2010). Proteomics analysis of the nucleolus in
adenovirus-infected cells. Mol. Cell. Proteomics 9:117-130.
(VPK-100)
9. Sukumaran S. et al. (2009). Functional characterization of the
human 1-acylglycerol-3-phosphate-O-acyltransferase isoform
10/glycerol-3-phosphate acyltransferase isoform 3. J. Mol.
Endocrinol. 42:469-478. (VPK-100)
10.Li, H. et al. (2009). Nuclear translocation of Ad/EYFP-hCAR: a
novel tool for screening human CAR activators in human primary hepatocytes. Drug Metab. Dispos. 37:1098-1106. (VPK100)
11.Guan, M. et al. (2008). Adenovirus-mediated restoration of
expression of the tumor suppressor gene DLC1 inhibits the
proliferation and tumorigenicity of aggressive, androgenindependent human prostate cancer cell lines: prospects for
gene therapy. Cancer Gene Ther. 15:371-381. (VPK-100)
Selection Guide for ViraBind Adenovirus Purification Kits
Purification Method
Purification Time
Capacity/Prep (Viral Particles)
Capacity/Prep (Supernatant)
Product Name
ViraBind Adenovirus Miniprep Kit
ViraBind Adenovirus Purification Kit
46
Phone 1-858-271-6500
ViraBind Adenovirus
Miniprep Kit
ViraBind Adenovirus
Purification Kit
Spin column
Syringe filter
30 minutes
30 minutes
11
1 x 10 VP
2.5 x 1012 VP
One T75 flask

or one 10cm dish
Four T75 flasks
Capacity/Prep
1 x 10
11
2.5 x 10
VP
12
VP
Size
Catalog Number
10 Preps
VPK-099
10 Preps
VPK-100
Fax 1-858-271-6514
VIRAL EXPRESSION
QuickTiter Adenoviral Titer & Quantitation Kits

Accurate measurement of virus titer is critical for viral
gene delivery. Traditional plaque-forming unit (PFU)
assays are long and have high inter-assay variability.
The QuickTiter Adenovirus Titer Kits provide a
complete system to functionally titer virus infectivity
with greater accuracy in a fraction of the time. The
assays may be used with any adenovirus system that
can amplify in 293 cells. Assays are available for ICC
staining or 96-well ELISA.
For a quick test of physical titer, our QuickTiter
Adenovirus Quantitation Kit measures the concentration of your adenovirus prep in about one hour.
QuickTiter Adenovirus Titer Immunoassay Kit. 293AD cells

(p. 42) were infected with different dilutions of purified Ad--Gal for
48 hours. Immunostaining was performed according to the assay
protocol. X-gal staining was performed with -Galactosidase Staining Kit (p. 106).
Faster, More Accurate and Precise: Compared to

traditional plaque-forming unit assays
User-Friendly Protocol: No agar overlay steps
Versatile: Recognize all 41 adenovirus serotypes
1. Smith, M. et al. (2010). PRDM1/Blimp-1 controls effector cytokine
production in human NK cells. J. Immunol. 185:6058-6067. (VPK106)
2. Reiter, C.E.N. et al. (2010). Green tea polyphenol epigallocatechin gallate reduces endothelin-1 expression and secretion in
vascular endothelial cells: roles for AMP-activated protein kinase,
Akt, and FOXO1. Endocrinology 151:103-114. (VPK-106)
3. Polling, J. et al. (2011). Induction of smooth muscle cell migration during arteriogenesis is mediated by Rap2. Arterioscler.
Thromb. Vasc. Biol. 31:2297-2305. (VPK-109)
4. Triulzi, C. et al. (2010). Antibody-dependent natural killer cellmediated cytotoxicity engendered by a kinase-inactive HER2
adenovirus-based vaccination mediates resistance to breast
tumors. Cancer Res. 70:7431-7441. (VPK-109)
5. Peled, M. et al. (2009). Systemic administration of a conditionally
replicating adenovirus, targeted to angiogenesis, reduced lung
metastases burden in cotton rats. Clin. Cancer Res. 15:16641673. (VPK-109)
6. Troidl, K. et al. (2009). Actin-binding Rho activating protein (Abra)
is essential for fluid shear stress-induced arteriogenesis. Arterioscler. Thromb. Vasc. Biol. 29(12):2093-2101. (VPK-109)
7. Chang, C-T. et al (2008). TGF-1 decreases epithelial sodium
channel functionality in renal collecting duct cells using a Smad4dependent pathway. Nephrol. Dial. Transplant. 23:1126-1134.
(VPK-109)
8. Muruganandan, S. et al. (2011). Chemerin, a novel peroxisome
proliferator-activated receptor gamma (PPARgamma) target gene
that promotes mesenchymal stem cell adipogenesis. J. Biol.
Chem. 286:23982-23995. (VPK-110)
9. Hoashi, T. et al. (2010). The secreted form of a melanocyte membrane-bound glycoprotein (Pmel17/gp100) is released by ectodomain shedding. FASEB J. 24(3):916-930. (VPK-110)
Selection Guide for QuickTiter Adenoviral Quantitation Kits
Functional or
Physical Titer
QuickTiter Adenovirus
Titer Immunoassay Kit
Titer ELISA Kit
Quantitation Kit
Functional
(Infectious units)
Functional
(Infectious units)
Physical
(Viral particles)
2.5 days
2.5 days
45-60 minutes
Antibody-based
Antibody-based
Total nucleic acid content
Immunocytochemical
staining
Colorimetric (ELISA)
plate reader
Fluorescence
plate reader
Accuracy
Accuracy
Speed
Assay Time
Assay Principle
Detection Method
Key Benefit
Product Name
Detection
Size
Catalog Number
QuickTiter Adenovirus Titer Immunoassay Kit
ICC Staining
100 Assays
VPK-109
QuickTiter Adenovirus Titer ELISA Kit
Colorimetric
2 x 96 Assays
VPK-110
QuickTiter Adenovirus Quantitation Kit
Fluorometric
20 Assays
VPK-106
www.cellbiolabs.com
47
VIRAL EXPRESSION
Rapid Replication Competent Adenovirus (RCA) Assay Kit

This kit uses the assay principles of the QuickTiter
Adenovirus Titer Immunoassay Kit (see page 47), but
is designed specifically to measure the level of replication-competent virus in your adenoviral prep.
Immunostaining of Wild
Type Ad5 using the
Rapid RCA Assay Kit.
Faster Results: 2.5 days vs. 10 days with plaque

assay
Versatile: Recognizes all 41 adenovirus serotypes
Product Name
Detection
Rapid RCA Assay Kit
Size
Catalog Number
30 Assays
VPK-111
5 x 30 Assays
VPK-111-5
ICC Staining
ViraDuctin Adenovirus Transduction Reagent, CAR-Independent

Adenovirus infection of target cells is mediated
largely by the coxsackievirus-adenovirus receptor
(CAR). Generally adenoviral transduction of many
immortalized cell lines proceeds with a high level of
efficiency. However, in many primary cells this receptor is either absent or present at extremely lowlevels. This can reduce the efficiency of adenovirus
transduction into your cell of choice.
Higher Transduction Efficiency: Up to 12-fold

increase in adenoviral uptake
User-Friendly: Short incubation step prior to
host cell infection
Versatile: Ideal for target cells expressing little
or no CAR, but may also improve transduction
efficiency for CAR-expressing cells
1. Ackerman, W. et al (2008). Nuclear Factor-kappa B regulates inducible prostaglandin E synthase expression in human amnion mesenchymal cells. Biol. Reprod. 78:68-76.
(AD-200, AD-201)
2. Monick, M. et al (2008). Constitutive ERK MAPK activity
regulates macrophage ATP production and mitochondrial
integrity. J. Immunol. 180:7485-7496. (AD-200)
1400
X-Gal Staining Positive (%)
ViraDuctin Adenovirus Transduction Reagent is

designed specifically to increase the efficiency of
adenoviral transduction, without regard to the level
of CAR expression on the surface of the target cells.
1200
1000
800
600
400
200
0
Control
ViraDuctin
Enhanced Transduction using ViraDuctin Adenovirus Transduction Reagent. Infection of NIH3T3 cells with recombinant Ad-
-gal (ADV-002). Top: X-gal staining under microscope. Bottom:
scoring of infection with ViraDuctin reagent as a percentage of
infection with control.
Product Name
Size*
Catalog Number
10 Transductions
AD-200
50 Transductions
AD-201
ViraDuctin Adenovirus Transduction Reagent (CAR-Independent)

*Based on using 6-well plates or 35mm culture dishes; may also be used with 96-,24- or 12-well plates or 60mm or 100mm dishes.
48
Phone 1-858-271-6500
Fax 1-858-271-6514
Lentiviral Expression
VIRAL EXPRESSION
Lentiviral Expression Kits & Reagents

As a sub-class of retroviruses, lentiviruses based on HIV-1 have the unique advantage of
being able to infect both proliferating and non-proliferating cells, and they can be used for
both transient and stable gene expression.
We offer a complete workflow solution to your lentiviral expression studies:
Expression Systems & Vectors

Premade Controls
Viral Host Cell Line
Concentration / Purification Kits

ViraSafe Lentiviral Expression Systems

Lentiviruses based on HIV-1 may infect both dividing
and non-dividing cells. Recently developed thirdgeneration lentiviral expression systems have reduced the risk of creating replication-competent virus
upon recombination, but the risk is still present.
Our ViraSafe Lentiviral Expression Systems provide a safer and more flexible method to package
your lentivirus, even compared to other thirdgeneration lentivirus systems.
Safer: 80-90% less sequence homology compared
to other 3rd-generation lentiviral systems; ecotropic
systems provide even more safety*
High Titers: Incorporates elements that provide
titers comparable to other 3rd-generation systems
Flexible: Packaging vectors provided separately for
increased safety and optimization of vector ratios
*Lentiviruses made with a ViraSafe

Ecotropic Expression System will only
readily infect mouse and rat cells. Pantropic lentiviruses are VSVG-pseudotyped
and may infect cells of any species.
ViraSafe Lentiviral Technology is available in three
formats (see next two pages for ordering information):
Complete Expression Systems: Include 3 packaging plasmids, expression vector and control vector
Packaging Systems: Include the 3 individual packaging plasmids; ideal if you already have a 3rdgeneration lentiviral expression construct
Expression Vectors: Nine cloning vectors to choose
from; compatible with any 2nd or 3rd generation
packaging system
Lentivirus Production using the ViraSafe Lentiviral

Expression System.
www.cellbiolabs.com
49
VIRAL EXPRESSION
ViraSafe Lentiviral Expression Systems, continued

Complete ViraSafe Expression Systems include three individual packaging plasmids, an expression vector,
and a control vector. Choose an ecotropic system for infection of mouse or rat cells; VSVG for any species.
Product Name
Envelope
Size
Catalog Number
Ecotropic
1 Kit
VPK-211-ECO
Pantropic (VSVG)
1 Kit
VPK-211-PAN
Ecotropic
1 Kit
VPK-212-ECO
Pantropic (VSVG)
1 Kit
VPK-212-PAN
Ecotropic
1 Kit
VPK-213-ECO
Pantropic (VSVG)
1 Kit
VPK-213-PAN
Ecotropic
1 Kit
VPK-214-ECO
Pantropic (VSVG)
1 Kit
VPK-214-PAN
Ecotropic
1 Kit
VPK-215-ECO
Pantropic (VSVG)
1 Kit
VPK-215-PAN
Ecotropic
1 Kit
VPK-216-ECO
Pantropic (VSVG)
1 Kit
VPK-216-PAN
Ecotropic
1 Kit
VPK-217-ECO
Pantropic (VSVG)
1 Kit
VPK-217-PAN
Ecotropic
1 Kit
VPK-218-ECO
Pantropic (VSVG)
1 Kit
VPK-218-PAN
Ecotropic
1 Kit
VPK-219-ECO
Pantropic (VSVG)
1 Kit
VPK-219-PAN
Ecotropic
1 Kit
VPK-220-ECO
Pantropic (VSVG)
1 Kit
VPK-220-PAN
ViraSafe Universal Lentiviral Expression System (Promoterless)
ViraSafe Lentiviral Expression System (Puro)
ViraSafe Lentiviral Expression System (Neo)
ViraSafe Lentiviral Expression System (Hygro)
ViraSafe Lentiviral Bicistronic Expression System (Puro)
ViraSafe Lentiviral Bicistronic Expression System (Neo)
ViraSafe Lentiviral Bicistronic Expression System (Hygro)
ViraSafe Lentiviral Bicistronic Expression System (GFP)
ViraSafe Lentiviral Bicistronic Expression System (Blasticidin)
ViraSafe miRNA Lentiviral Expression System
ViraSafe Lentiviral Packaging Systems

ViraSafe Packaging Systems contain 3 packaging plasmids for use with any 3rd-generation lentiviral expression vector. These systems are perfect if you already have a lentiviral construct containing your gene of interest.
Product Name
Envelope
Size
Catalog Number
Ecotropic
1 Kit
VPK-205
Pantropic (VSVG)
1 Kit
VPK-206
ViraSafe Lentiviral Packaging System
293LTV Lentiviral Cell Line

Shimamura, T. et al (2008). Hsp90 inhibition suppresses mutant EGFR-T790M signaling and overcomes kinase inhibitor resistance.
Cancer Res. 68:5827-5838. (LTV-100)
Product Name
Phone 1-858-271-6500
Catalog Number
LTV-100
1 x 10 Cells
293LTV Cell Line
50
Size
Fax 1-858-271-6514
VIRAL EXPRESSION
ViraSafe Lentiviral Expression Vectors
These lentiviral expression vectors contain multiple
cloning sites to accommodate your gene of interest.
Each vector may be used with any 2nd or 3rd generation lentiviral packaging system including our
ViraSafe Lentiviral Packaging Systems. Each
vector can accommodate large inserts of up to at
least 7 kb.*

Sankaran, V.G. et al. (2011).
MicroRNA-15a and -16-1 act
via MYB to elevate hemoglobin
expression in human trisomy
13. PNAS 108(4):1519-1524.
(VPK-212)
*Our Universal (Promoterless) vector can accommodate inserts of

up to 10 kb total (including promoter).
Product Name
Size
Catalog Number
pSMPUW Universal Lentiviral Expression Vector (Promoterless)
10 g
VPK-211
pSMPUW-Puro Lentiviral Expression Vector
10 g
VPK-212
pSMPUW-Neo Lentiviral Expression Vector
10 g
VPK-213
pSMPUW-Hygro Lentiviral Expression Vector
10 g
VPK-214
pSMPUW-IRES-Puro Lentiviral Expression Vector
10 g
VPK-215
pSMPUW-IRES-Neo Lentiviral Expression Vector
10 g
VPK-216
pSMPUW-IRES-Hygro Lentiviral Expression Vector
10 g
VPK-217
pSMPUW-IRES-GFP Lentiviral Expression Vector
10 g
VPK-218
pSMPUW-IRES-Bsd Lentiviral Expression Vector
10 g
VPK-219
pSMPUW miRNA Lentiviral Expression Vector
10 g
VPK-220
Product Name
Size
Catalog Number
pLenti-GFP Lentiviral Control Vector
10 g
LTV-400
pSMPUW-GFP-Puro Lentiviral Control Vector
10 g
LTV-401
pSMPUW-MNDnLacZ Lentiviral Control Vector
10 g
LTV-402
pLenti-RFP-Puro Lentiviral Control Vector
100 L
LTV-403
Lentiviral Control Plasmids

These plasmids allow you to package your own reporter lentivirus.
Premade Reporter Lentivirus Controls

Lentiviral Transduction of 293
Cells. Left: GFP
Lentivirus Control
Right: -Gal Lentivirus Control.
These controls provide the convenience of a premade virus packaged with a reporter gene. Each
virus is provided at a predetermined concentration.
Product Name
Concentration
Size
Catalog Number
GFP Lentivirus Control
1 x 106 TU/mL
200 L
LTV-300
RFP Lentivirus Control
1 x 106 TU/mL
200 L
LTV-301
500 L
LTV-302
-Galactosidase Lentivirus Control
1 x 10 TU/mL
www.cellbiolabs.com
51
VIRAL EXPRESSION
QuickTiter Lentivirus Titer / Quantitation Kits

Measuring lentiviral titer is important prior to infection
of your target cells, and one of the most published
methods is the p24 ELISA. Our traditional p24 ELISA
kits provide a quick, convenient way to quantify the
concentration of your lentivirus. Individual kits are
available to measure either HIV-1 or FIV concentrations.
One disadvantage of using a traditional p24 ELISA to
quantify lentivirus is the overexpression of p24 during
lentiviral packaging. Free p24 protein may account for
a substantial portion of total p24 in lentiviral supernatant. The traditional p24 ELISA detects both virusassociated p24 and free p24 generated by 293T cells
during transient transfection. Our QuickTiter Lentivirus Titer Kit minimizes the overestimation of p24 in
lentivirus supernatant. Our proprietary technology
separates the lentivirus-associated p24 from free p24
protein prior to performing the ELISA.
If you need a very quick estimate of your lentiviral
concentration, try the QuickTiter Lentivirus Quantitation Kit. This kit specifically measures the viral nucleic acid content of purified virus or unpurified viral
supernatant. This method is ideal for a quick measurement of viral titer, either before or after purification
of your lentivirus.
More Accurate: Exclusive technology in QuickTiter Lentivirus Titer Kit minimizes overestimation of virus titer
User-Friendly: Read results on a standard
microplate reader
Assay Principle for the QuickTiter Lentivirus Titer Kit. Lentivirus particles are packaged with p24 protein, but additional free
p24 protein is present in viral supernatant. A traditional p24 ELISA
detects both sources of p24 which overestimates viral titer. The
QuickTiter Lentivirus Titer Kit uses technology to pull the virus
out of solution prior to quantitation for a more accurate viral titer.
Selection Guide for QuickTiter Quantitation & Titer Kits

QuickTiter
QuickTiter
Lentivirus Titer Kit
Lentivirus Quantitation Kits
(Lentivirus-Associated p24 ELISA)
(Traditional p24 ELISA)
Assay Principle
p24 ELISA with proprietary

technology to separate
free p24 from viral p24
p24 ELISA
Measures
nucleic acid content
Suitable Viruses
Recombinant HIV-1
Recombinant or native
HIV-1 or FIV
HIV-1, FIV, SIV
Detection Method
plate reader
plate reader
Fluorescence
plate reader
Accuracy
Most Published
Speed (45-60 min.)
Key Benefit
52
QuickTiter
Lentivirus
Quantitation Kit
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION
QuickTiter Lentivirus Titer / Quantitation Kits, continued

1.8
p24 in supernatant
1.6
p24 in pellet
OD 450nm
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0
25
50
75
100
p24 (ng/mL)
Free p24 Does not Complex with ViraBind Reagents. Recombinant p24 was diluted in culture medium and treated with ViraBind Lentivirus Reagents A and B found in the QuickTiter
Lentivirus Titer Kit. The amount of p24 in the supernatant and the
pellet was measured according to the assay protocol.
1.2
OD 450 nm
1
0.8
0.6
0.4
0.2
0
Culture medium
GFP Lentiviral
Supernatant
p24 Titer of GFP Lentiviral Supernatant. GFP lentiviral construct

was cotransfected with a packaging mix into 293 cells. The conditioned medium was harvested 48 hrs after transfection and used to
further infect 293 cells. The p24 level of the diluted lentiviral supernatant (1:10 dilution) was determined as described in the assay
protocol.
Product Name
Detection
QuickTiter Lentivirus Titer Kit (Lentivirus-Associated HIV p24 ELISA)
QuickTiter HIV Lentivirus Quantitation Kit (HIV-1 p24 ELISA)
QuickTiter FIV Lentivirus Quantitation Kit (FIV p24 ELISA)

QuickTiter Lentivirus Quantitation Kit

1. Keck, Z.-Y. et al. (2011). Mapping a region of Hepatitis C virus E2
that is responsible for escape from neutralizing antibodies and a
core CD81-binding region that does not tolerate neutralization
escape mutations. J. Virol. 85:10451-10463. (VPK-107)
2. Sanchez-Antequera, Y. et al. (2011). Magselectofection: an integrated method of nanomagnetic separation and genetic modification of target cells. Blood 117:e171-e181. (VPK-107)
3. Tiedemann, R.E. et al. (2010). Kinome-wide RNAi studies in human multiple myeloma identify vulnerable kinase targets, including a lymphoid restricted kinase, GRK6. Blood 115:1594-1604.
(VPK-107)
4. Lin, R-J. et al. (2009). Distinct antiviral roles for human 25oligoadenylate synthetase family members against dengue virus
infection. J. Immunol. 183:8035-8043. (VPK-107)
5. Sukumaran S. et al. (2009). Functional characterization of the
human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/
glycerol-3-phosphate acyltransferase isoform 3. J. Mol. Endocrinol. 42:469-478. (VPK-107)
6. Joshi, M.B. et al. (2009). Extracellular cadherin repeat domains
EC1 and EC5 of T-cadherin are essential for its ability to stimulate
angiogenic behavior of endothelial cells. FASEB J. 23:4011-4021.
(VPK-107)
7. Keck, Z.-Y. et al. (2009). Mutations in HCV E2 located outside the
CD81 binding sites lead to escape from broadly neutralizing antibodies but compromise virus infectivity. J. Virol. 83:6149-6160.
(VPK-107)
8. Matsumae, H. et al. (2008). CCN1 knockdown suppresses neointimal hyperplasia in a rat artery balloon injury model. Arterioscler
Thromb Vasc Biol. 28(6):1077-83. (VPK-108-F)
9. Iftikhar, M. et al. (2011). Lysyl oxidase-like-2 (LOXL2) is a major
isoform in chondrocytes and is critically required for differentiation. J. Biol. Chem. 286:909-918. (VPK-108-H)
10. Agrawal-Gamse, C. et al. (2010). Yeast-elicited cross-reactive to
HIV Env glycans efficiently neutralize virions expressing exclusively high mannose N-linked glycans. J. Virol. 85(1):470-480.
(VPK-108-H)
11. Young, A.P. and Wagers, A.J. (2010). Pax3 induces differentiation of juvenile skeletal muscle stem cells without transcriptional
upregulation of canonical myogenic regulatory factors. J. Cell Sci.
123:2632-2639. (VPK-108-H)
12. Kaletsky, R. et al. (2009). Tetherin-mediated restriction of filovirus
budding is antagonized by the Ebola glycoprotein. PNAS
106:2886-2891. (VPK-108-H)
13. Veeraraghavalu, K. et al. (2010). Presinilin 1 mutants impair the
self-renewal and differentiation of adult murine subventricular
zone-neuronal progenitors via cell-autonomous mechanisms
involving notch signaling. J. Neurosci. 30:6903-6915. (VPK-112)
14. Niwano, K. et al. (2008). Lentiviral vector-mediated SERCA2
gene transfer protects against heart failure and left ventricular
remodeling after myocardial infarction in rats. Mol. Ther. 16:10261032. (VPK-112)
Size
Catalog Number
96 Assays
VPK-107
5 x 96 Assays
VPK-107-5
96 Assays
VPK-108-H
5 x 96 Assays
VPK-108-H-5
96 Assays
VPK-108-F
5 x 96 Assays
VPK-108-F-5
20 Assays
VPK-112
Colorimetric
Colorimetric
Colorimetric
Fluorometric
www.cellbiolabs.com
53
VIRAL EXPRESSION
ViraBind Lentivirus Concentration & Purification Kits

Ultracentrifugation methods used for lentiviral supernatants are tedious and time-consuming and usually
only partially purify your virus. Alternatively, filterbased methods have low sample capacity and do
not substantially concentrate the virus.
ViraBind Lentivirus Concentration and Purification
Kits produce purified lentivirus with extremely high
titer without the need for ultracentrifugation. The virus
is pelleted from solution using our proprietary isolation technology, then resuspended in a smaller volume. The resuspended lentivirus is then applied to
either a purification column or a dialysis device for
purification. If desired, the purified virus may be further concentrated using a centrifugal concentrator.
Fast: Obtain purified virus in about 4-6 hours

with column-based kits and 10-24 hours with
dialysis-based kits
High Titer: Concentrate up to 500-fold to as
high as 108-1010 TU/ml, sufficient for in vivo
studies
High Yield: Recover >60%
Lentivirus Concentration and Purification Procedure.
Selection Guide for Lentivirus Concentration & Purification Kits

ViraBind
Lentivirus
Concentration and
Purification Kit
Purification
Method
ViraBind PLUS
Lentivirus
Concentration and
Purification Kit
ViraBind PLUS
Lentivirus
Concentration and
Purification Mega Kit
Proprietary Reagent Cocktail Proprietary Reagent Cocktail Proprietary Reagent Cocktail

+ Purification Column
+ Dialysis
+ Dialysis
Total Time
Capacity per Prep
(Supernatant)
6-8 hours
10-24 hours
10-24 hours
100 mL
50 mL
500 mL
Product Name
ViraBind Lentivirus Concentration and Purification Kit (100 ml/prep)
ViraBind PLUS Lentivirus Concentration and Purification Kit (50 ml/prep)
Size
Catalog Number
2 Preps
VPK-090
5 Preps
VPK-091
25 Preps
VPK-091-5
2 Preps
VPK-095
2 Preps
VPK-096
10 Preps
VPK-096-5
ViraBind PLUS Lentivirus Concentration and Purification Mega Kit (500 ml/prep)
54
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION

1. McEachron, T.A. et al. (2010). Protease-activated receptors
mediate crosstalk between coagulation and fibrinolysis. Blood
116:5037-5044.
2. Zemskova, M. et al. (2010). p53-dependent induction of prostate
cancer cell senescence by the PIM1 protein kinase. Mol. Cancer
Res. 8:1126-1141.
Polybrene
500
400
ViraDuctin
300
200
100
H
T10
80
29
3
H
eL
a
Higher Transduction Efficiency: 2-6x higher

in many cell lines compared to Polybrene
More Robust: Useful for transduction of nonpermissive cells, including primary cells and
stem cells
600
Our ViraDuctin Lentivirus Transduction Kit provides

superior transduction efficiencies in a variety of cell
lines, even when compared to transductions in the
presence of Polybrene. The ViraDuctin system is
ideal for primary cells and stem cells.
700
N
IH
3T
Lentivirus transduction efficiency is typically low. Additives such as Polybrene can boost transduction
efficiencies, but even then only a small fraction of lentiviral vectors can transduce many target cell lines.
Normalized GFP Fluorescence
ViraDuctin Lentivirus Transduction Kit
Transduction Efficiencies in Various Cell Lines. NIH3T3

cells, HeLa cells, our own 293AD cells (page 36) and HT-1080
cells were each seeded at 50,000 cells/well in a 24-well plate
overnight. Cells were infected with GFP lentivirus for 48 hours in
the presence of Polybrene or ViraDuctin Lentivirus Transduction Kit. For each cell line, fluorescence levels using the ViraDuctin system are depicted relative to a normalized fluorescence of 100 for Polybrene.
Polybrene is a registered trademark of Abbott Laboratories.
Transduction of 293AD and HT-1080 Cells. 293AD cells (p. 36) and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well
plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of no additive (left), Polybrene (middle) or the ViraDuctin reagent cocktail (right).
Product Name
Size*
Catalog Number
40 Transductions
LTV-200
200 Transductions
LTV-201
ViraDuctin Lentivirus Transduction Kit

*Based on a 24-well plate. Can also be used with 96-well, 12-well or 6-well plates, as well as 60mm or 100mm dishes. See product insert.
www.cellbiolabs.com
55
VIRAL EXPRESSION
Retroviral Expression
Retroviral Expression Kits & Reagents

Traditional retroviral vectors based on MMLV are useful for integrating genetic material
into the host cell genome. However, retrovirus titer tends to be significantly lower than
that of adenovirus, which can lead to a lower infection efficiency.
Our retroviral reagents and kits incorporate technologies that increase your chances of
successful retroviral expression. We offer a comprehensive solution from start to finish:
Gene-Specific Retroviral Vectors
Retroviral Packaging Cell Lines
Retroviral Cloning & Expression
Concentration / Purification Kits
Vectors
Retroviral Expression Systems
Gene-Specific Recombinant Retroviral Vectors

These constructs are based on backbones derived
from the Moloney murine leukemia virus (MMLV). Vectors with GFP or iPS stem cell factors are supplied as
10 g of plasmid in TE buffer. All other vectors are supplied as 100 L of bacterial glycerol stock.
Reporter Genes
Target Name
Vector Backbone
Catalog Number
GFP
pBABE
RTV-002
GFP
pMCs
RTV-051
GFP
pMX
RTV-050
GFP
pMYs
RTV-052
GFP-Puro
pMX
RTV-053
Cell Cycle
Huang, J. et al. (2009). Regulation of the leucocyte chemoattractant receptor FPR in glioblastoma cells by cell differentiation.
Carcinogenesis 30(2):348-355. (RTV-401)
Target Name
Vector Backbone
Catalog Number
c-Abl
pBABEpuro
RTV-402
c-Abl-TM
pBABEpuro
RTV-403
c-Abl (1-565)
pBABEpuro
RTV-404
c-Abl (1-958)
pBABEpuro
RTV-405
pBABEhygro
RTV-007
pBABEneo
RTV-005
pBABEpuro
RTV-006
Target Name
pBABEpuro
RTV-401
GFP-LC3
hTERT
p53
56
Phone 1-858-271-6500
Generic Map of pBABEhygro Retroviral Expression Vector.
Autophagy
This vector is supplied with a separate pMXs-GFP
control vector at no additional cost.
Vector Backbone
Catalog Number
pMXs
RTV-801
Fax 1-858-271-6514
VIRAL EXPRESSION
Gene-Specific Recombinant Retroviral Vectors, continued

Cytoskeleton Regulation
Target Name
Vector Backbone
Mutation State
Catalog Number
pBABEhygro
L61
RTV-203
pBABEpuro
N/A
RTV-220
pWZLhygro
Q61
RTV-221
myr-Rac1
pBABEpuro
N/A
RTV-201
Rac1
pBABEhygro
V12
RTV-202
N-Ras
pBABEpuro
K61
RTV-222
Rac3
pBABEhygro
V12
RTV-205
pBABEpuro
V12
RTV-101
pBABEpuro
V12C40
RTV-104
pBABEpuro
V12G37
RTV-103
pBABEpuro
V12S35
RTV-102
pBABEhygro
L63
RTV-204
Cdc42
K-Ras
Ras
RhoA
iPS / Stem Cell Factors

Human iPS Genes
Mouse iPS Genes
Target Name
Vector Backbone
Catalog Number
Target Name
Vector Backbone
Catalog Number
4-Vector Set*
pMXs
RTV-701-C
4-Vector Set*
pMXs
RTV-705-C
6-Vector Set**
pMXs
RTV-709-C
6-Vector Set**
pMXs
RTV-711-C
c-Myc
pMXs
RTV-703
c-Myc
pMXs
RTV-707
Klf4
pMXs
RTV-704
Klf4
pMXs
RTV-708
Lin-28
pMXs
RTV-710
Lin-28
pMXs
RTV-712
NANOG
pMXs
RTV-709
NANOG
pMXs
RTV-711
Oct-3/4
pMXs
RTV-701
Oct-3/4
pMXs
RTV-705
Sox2
pMXs
RTV-702
Sox2
pMXs
RTV-706
p53 shRNA
pRetro
RTV-410
p53 shRNA
pRetro
RTV-400
*4-Vector sets contain individual constructs with the following genes: c-Myc, Klf4, Oct-3/4 and Sox2.
**6-Vector sets contain individual constructs with the following genes: c-Myc, Klf4, Oct-3/4, Sox2, Lin-28 and NANOG.
Proteases and Related Molecules

Target Name
Vector Backbone
Catalog Number
uPA
pBABEpuro
RTV-501
uPAR
pBABEhygro
RTV-502

Gutova, M. et al (2008). Urokinase plasminogen activator and
urokinase plasminogen activator receptor mediate human stem
cell tropism to malignant solid tumors. Stem Cells 26:1406-1413.
(RTV-501, RTV-502)
www.cellbiolabs.com
57
VIRAL EXPRESSION
Gene-Specific Recombinant Retroviral Vectors, continued

Vector Name
Vector Backbone
Mutation State
Catalog Number
ERK2
pBABEhygro
Dominant Negative
RTV-109
JNK1
pBABEpuro
Dominant Negative
RTV-110
pBABEpuro
Constitutively Active
RTV-118
pBABEpuro
Dominant Negative
RTV-119
pBABEpuro
RTV-120
pBABEpuro
Dominant Negative
RTV-121
pBABEhygro
RTV-112
pBABEhygro
Dominant Negative
RTV-111
pBABEpuro
RTV-114
pBABEhygro
Dominant Negative
RTV-115
pBABEpuro
RTV-116
pBABEhygro
Dominant Negative
RTV-117
pWZLneo
RTV-125
p38
pBABEhygro
Dominant Negative
RTV-105
p38
pBABEhygro
Dominant Negative
RTV-106
p38
pBABEhygro
Dominant Negative
RTV-107
p38
pBABEhygro
Dominant Negative
RTV-108
pWZLneo
RTV-124
pBABEpuro
RTV-122
pBABEpuro
Dominant Negative
RTV-123
pWZLneo
RTV-113
MAPKAPK2
MAPKAPK3
MEK1
MKK3
MKK6
myr-Akt1
PI3K p110-CAAX
PRAK
Raf1-CAAX
Transcription Regulation
Target Name
58
Vector Backbone
Catalog Number
AUF1
pBABEpuro
RTV-305
hnRNPA0
pBABEpuro
RTV-310
hnRNP-A2
pBABEpuro
RTV-340
HuB
pBABEpuro
HuC
Vector Backbone
Catalog Number
Stat5A(1*6)
pMXs
RTV-331
Stat5A-IRES-GFP
pMXs
RTV-332
pMXs
RTV-333
RTV-302
Stat5A(1*6)-IRESGFP
pBABEpuro
RTV-303
Stat5B
pMXs
RTV-334
HuD
pBABEpuro
RTV-301
Stat5B(1*6)
pMXs
RTV-335
HuR
pBABEpuro
RTV-304
TIA-1
pBABEpuro
RTV-309
PABP
pBABEpuro
RTV-307
TIAR
pBABEpuro
RTV-308
Stat5A
pMXs
RTV-330
TTP
pBABEpuro
RTV-306
Phone 1-858-271-6500
Target Name
Fax 1-858-271-6514
VIRAL EXPRESSION
Retroviral Cloning & Expression Vectors

Our Retroviral Expression Vectors contain multiple
cloning sites (MCS) to allow easy introduction of your
gene of interest. The vectors are based on backbones
derived from Moloney murine leukemia virus (MMLV).
We offer the traditional pBABE system and the newer
pMXs system, which has been shown to be useful in
induced pluripotent stem cell (iPS) studies. pMYs vectors are optimal for use with hematopoietic stem cells,
and pMCs vectors are optimal for ES and EC cells.
All cloning vectors are supplied as 10 g in TE buffer.
1. Ding, J. et al. (2010). Bcl-2 and Bax interact via the BH-1-3
groove-BH3 motif interface and a novel interface involving the
BH4 motif. J. Biol. Chem. 285:28749-28763. (RTV-001-HYGRO)
2. Jeong, H. et al. (2010). TAZ as a novel enhancer of MyoDmediated myogenic differentiation. FASEB J. 24(9):3310-3320.
(RTV-001-PURO)
3. Miyoshi, N. et al. (2010). Defined factors induce reprogramming
of gastrointestinal cancer cells. PNAS 107:40-45. (RTV-010)
4. Iwai, A. et al. (2010). Influenza A virus polymerase inhibits type I
interferon induction by binding to interferon promoter stimulator 1. J. Biol. Chem. 285:32064-32074. (RTV-012)
5. Guan, Y. et al. (2010). Human TLRs 10 and 1 share common
mechanisms of innate immune sensing but not signaling. J.
Immunol. 184:5094-5103. (RTV-013)
6. Cui, G. et al. (2009). Berberine differentially modulates the activities of ERK, p38 MAPK, and JNK to suppress Th17 and Th1
cell differentiation in type 1 diabetic mice. J. Biol. Chem.
284:28420-28429. (RTV-013)
7. Sugatani, T. and K. Hruska (2009). Impaired microRNA pathways diminish osteoclast differentiation and function. J. Biol.
Chem. 284:4667-4678. (RTV-014)
8. Sugatani, T. et al. (2011). A microRNA expression signature of
osteoclastogenesis. Blood 117:3648-3657. (RTV-014, RTV-016)
9. Malicet, C. et al. (2011). Distinct properties of human HMGN5
reveal a rapidly evolving but functionally conserved nucleosome
binding protein. Mol. Cell Biol. 31:2742-2755. (RTV-040)
Vector Name
Catalog Number
pBABEhygro
RTV-001-HYGRO
pBABEneo
RTV-003
pBABEpuro
RTV-001-PURO
pBABEzeo
RTV-004
pMCs-IRES-GFP
RTV-040
pMCs-Puro
RTV-041
pMXs
RTV-010
pMXs-miR-GFP/Puro
RTV-017
pMXs-IRES-Blasticidin
RTV-016
pMXs-IRES-GFP
RTV-013
pMXs-IRES-Neo
RTV-015
pMXs-IRES-Puro
RTV-014
pMXs-Neo
RTV-011
pMXs-Puro
RTV-012
pMYs
RTV-020
pMYs-IRES-GFP
RTV-021
pMYs-IRES-Neo
RTV-023
pMYs-IRES-Puro
RTV-022
pMYs-Puro
RTV-024
pMZs
RTV-030
pWZL-Neo
RTV-001-NEO
Retroviral Packaging Vectors and Cells

These constructs are ideal for researchers who prefer
a traditional multi-plasmid transfection of 293 cells for
packaging recombinant retrovirus. Our 293RTV cells
are derived from the 293 parental cell line, but are
selected for firmer attachment to culture plates, faster
growth and higher yields of retrovirus produced.

Oida, T. et al. (2010). Overexpression of TGF-1 gene induces
cell surface localized glucose-regulated protein 78-associated
latency-associated peptide/TGF-. J. Immunol. 185:3529-3535.
(RV-110)
Product Name
Size
Catalog Number
pCMV-10A1 Envelope Vector
100 L
RV-114
pCMV-Ampho Envelope Vector
100 L
RV-113
pCMV-Eco Envelope Vector
100 L
RV-112
pCMV-Gag-Pol Retroviral Vector
10 g
RV-111
pCMV-VSV-G Envelope Vector
10 g
RV-110
>1 x 10 cells
293RTV Cell Line
www.cellbiolabs.com
RV-100
59
VIRAL EXPRESSION
Platinum Retroviral Expression Systems

Retroviral vectors are useful for delivering genes of
interest into a host cell where integration into the genome is desired. However, traditional retroviral expression technologies usually result in low viral titers,
making gene expression studies difficult. This is
mainly due to poor expression of retroviral structure
proteins (e.g. gag, pol, and env) in the cells.
Our Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid
transfection. Each Platinum Expression System includes one of our exclusive Platinum Packaging Cell
Lines which stably express the gag and pol genes. In
the Ecotropic and Amphotropic systems, the packaging cells also express the envelope protein.* Simply
clone your gene of interest into the vector provided
and transfect into the Platinum cells.
*Pantropic systems require co-transfection with VSVG
envelope vector.
Higher Viral Yields: Average titer 107 infectious

units/mL with transient transfection
Longer Stability: Expression up to 4 months in the
presence of drug selection
Optimized Systems: 3 packaging cell lines for infection of various species; 3 vector backbones (two
specifically for infection of stem cells)
Flexible: Order complete systems or cells and vectors separately
Not sure which Platinum Expression System is right
for you? See the table below for a selection guide
based on the host species of your target cell.
Plat-A Cells
(Amphotropic)
Plat-E Cells
(Ecotropic)
Plat-GP Cells
(Pantropic*)
Human
+++
N.S.
+++
Mouse
+++
+++
+++
Rat
+++
+++
+++
Monkey
+++
N.S.
+++
Cat
+++
N.S.
+++
Dog
+++
N.S.
+++
N.S.
+++
Bird
N.S.
N.S.
+++
Fish
N.S.
N.S.
+++
Frog
N.S.
N.S.
+++
Insect
N.S.
N.S.
+++
Mollusk
N.S.
N.S.
+++
Hamster
*Virus must be packaged with a pantropic envelope protein such as

VSV-G.
N.S. = Not Suitable
Suitability of Platinum Retroviral Packaging Cell Lines by Host
Species.
High Viral Yields

with Plat-E Cells.
ProB Ba/F3 cells
were infected with
GFP retrovirus
supernatant produced in Plat-E
cells after transfection with pMX-GFP
vector.
Retrovirus Production Using the Platinum Retroviral

Expression Systems.
60
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION
Platinum Retroviral Expression Systems, continued

Platinum Retroviral Expression Systems contain everything you need to generate your recombinant retrovirus:
packaging cell line, expression vector, and GFP control vector. Our pantropic systems also contain a VSVG envelope vector. Simply clone your gene of interest into the expression vector.
These complete systems offer the greatest value, but you may also order the Platinum Packaging Cell Lines and
Retroviral Expression Vectors separately on this page and the next.
Product Name
Expression Vector
Packaging Cell
Size
Catalog Number
Platinum Retroviral Expression System,

Ecotropic
pMXs-Puro
Plat-E
1 kit
VPK-300

Amphotropic
pMXs-Puro
Plat-A
1 kit
VPK-301

Pantropic
pMXs-Puro
Plat-GP
1 kit
VPK-302
Platinum ES/EC Retroviral Expression System,

Ecotropic
pMCs-Puro
Plat-E
1 kit
VPK-303

Amphotropic
pMCs-Puro
Plat-A
1 kit
VPK-304

Pantropic
pMCs-Puro
Plat-GP
1 kit
VPK-305
Platinum HSC Retroviral Expression System,

Ecotropic
pMYs-Puro
Plat-E
1 kit
VPK-306

Amphotropic
pMYs-Puro
Plat-A
1 kit
VPK-307

Pantropic
pMYs-Puro
Plat-GP
1 kit
VPK-308
Platinum Retroviral Packaging Cell Lines

Generate high titers of recombinant retrovirus with a
single plasmid transfection* using these extremely
powerful, stable cell lines. Platinum Retroviral Packaging Cells are based on the 293T cell line and exhibit
greater stability and produce higher yields of retroviral
structure proteins, resulting in higher retroviral titers.
The Platinum cell lines were invented in the laboratory
of Dr. Toshio Kitamura at the University of Tokyo and
are available exclusively from Cell Biolabs. They were
first described in the following paper:
Morita, S. et al. (2000). Gene Therapy 7:1063-1066.
*Plat-GP cells require co-transfection with VSVG envelope vector
(available separately).

1. Braunstein, M. et al. (2010). HEBALt enhances the T cell potential
of fetal myeloid-biased precursors. Int. Immunol. 22:963-972. (RV
-101)
2. Satpathy, S. et al. (2010). Inhibition of terminal differentiation of B
cells mediated by CD27 and CD40 involved signaling through
JNK. J. Immunol. 185:6499-6507. (RV-101)
3. Qiao, Y. et al. (2011). FOXQ1 regulates epithelial-mesenchymal
transition in human cancers. Cancer Res. 71:3076-3086. (RV102)
4. Hudecek, M. et al. (2010). The B-cell tumor-associated antigen
ROR1 can be targeted with T-cells modified to express a ROR1specific chimeric antigen receptor. Blood 116:4532-4541. (RV102)
5. Oda, Y. et al. (2010). Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. J. Biol. Chem.
285:29270-29278. (RV-102)
6. Cavnar, P.J. et al. (2011). Hax1 regulates neutrophil adhesion
and motility through RhoA. J. Cell Biol. 193:465-473. (RV-103)
Product Name
Size
6
Catalog Number
Platinum-E Retroviral Packaging Cell Line, Ecotropic
>3 x 10 cells
RV-101
Platinum-A Retroviral Packaging Cell Line, Amphotropic
>3 x 106 cells
RV-102
Platinum-GP Retroviral Packaging Cell Line, Pantropic

pVSV-G Packaging Vector
www.cellbiolabs.com
>3 x 10 cells
RV-103
10 g
RV-110
61
VIRAL EXPRESSION
ViraBind Retrovirus Concentration & Purification Kits

Ultracentrifugation methods used for lentiviral supernatants are tedious and time-consuming and usually
only partially purify your virus. Alternatively, filterbased methods have low sample capacity and do
not substantially concentrate the virus.
ViraBind Retrovirus Concentration and Purification
Kits produce purified lentivirus with extremely high
titer without the need for ultracentrifugation. The virus
is pelleted from solution using our proprietary isolation technology, then resuspended in a smaller volume. The resuspended retrovirus is then applied to
either a purification column or a dialysis device for
purification. If desired, the purified virus may be further concentrated using a centrifugal concentrator.
Fast: Obtain purified virus in about 4-6 hours

High Titer: Concentrate 500-fold to 109-1010
TU/ml, sufficient for in vivo studies
High Yield: Recover >60%
High Throughput: Process greater volumes
per prep than filter-based purification methods
Retrovirus Concentration and Purification Procedure.
Selection Guide for Retrovirus Concentration & Purification Kits

ViraBind
Retrovirus
Concentration and
Purification Kit
Purification
Method
ViraBind PLUS
Retrovirus
Concentration and
Purification Kit
ViraBind PLUS
Retrovirus
Concentration and
Purification Mega Kit
Proprietary Reagent Cocktail Proprietary Reagent Cocktail Proprietary Reagent Cocktail

+ Purification Column
+ Dialysis
+ Dialysis
Total Time
Capacity per Prep
(Supernatant)
6-8 hours
10-24 hours
10-24 hours
100 mL
50 mL
500 mL
Product Name
ViraBind Retrovirus Concentration and Purification Kit (100 ml/prep)
ViraBind PLUS Retrovirus Concentration and Purification Kit (50 ml/prep)
Size
Catalog Number
2 Preps
VPK-130
5 Preps
VPK-131
25 Preps
VPK-131-5
2 Preps
VPK-135
2 Preps
VPK-136
10 Preps
VPK-136-5
ViraBind PLUS Retrovirus Concentration and Purification Mega Kit (500 ml/prep)
62
Phone 1-858-271-6500
Fax 1-858-271-6514
VIRAL EXPRESSION
QuickTiter Retrovirus Rapid Quantitation Kit
This kit specifically measures the viral nucleic acid
content of purified virus or unpurified viral supernatant. This method is ideal for a quick measurement
of viral titer, either before or after purification of your
retrovirus.
Ultra-fast Results: 45-60 minute procedure
Convenient: Titer may be measured before purification step
Sensitive: Limit of detection = 1.5 x 109 VP/mL
from 2 mL of retroviral supernatant
500
RFU (520 nm)
400
300
200
100
0
0
400
800
1200
Retroviral RNA (ng)
80
RFU (520 nm)
70
60
50
40
30
20
10
0
0
50
100
150
Retroviral RNA (ng)

Retrovirus RNA Standard Curve. The QuickTiter Retrovirus
RNA Standard was diluted according to the assay protocol. Fluorescence was measured on a SpectraMax Gemini XS Fluorometer
(Molecular Devices) with a 485 / 538 nm filter set and a 530 nm
cutoff.
Product Name
QuickTiter Retrovirus Quantitation Kit
Assay Procedure for the QuickTiter Retrovirus

Quantitation Kit.
Detection
Size
Catalog Number
Fluorometric
20 Assays
VPK-120
www.cellbiolabs.com
63
VIRAL EXPRESSION
ViraDuctin Retrovirus Transduction Kit

The efficiency of retrovirus transduction can be low compared to other viruses. The rate at which retroviral vectors bind to cells is controlled mostly by diffusion. Additionally, the presence of transduction inhibitors such as
proteoglycans and glycosaminoglycans in retroviral supernatants can lead to poor gene transfer. Additives such
as Polybrene can boost transduction efficiencies, but they do not eliminate these transduction inhibitors.
Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies even when
compared to transductions in the presence of Polybrene. A proprietary reagent cocktail forms a supercomplex with the retrovirus which is pelleted away
from the supernatant, removing detrimental transduction inhibitors that decrease infection efficiency.
Polybrene is a registered trademark of Abbott Laboratories.
More Robust: Removes harmful transduction

inhibitors from retroviral supernatant
Higher Transduction Efficiencies: Compared
to infections in the presence of Polybrene or no
additives
Miyoshi, N. et al. (2010). Defined factors induce reprogramming of
gastrointestinal cancer cells. PNAS 107:40-45.
Product Name
Size*
Catalog Number
40 Transductions
RV-200
200 Transductions
RV-201
ViraDuctin Retrovirus Transduction Kit
*Number of transductions shown is based on use in a 24-well plate. This product may also be used with 96-well, 12-well or 6-well plates, as
well as 60mm or 100mm dishes. See product insert for specific details.
64
Phone 1-858-271-6500
Fax 1-858-271-6514
MICRORNA
ANALYSIS
miRNA Clone Collection
miRNASelect Precursor Clone Collection (Expression Vectors)

miRNASelect Expression Vectors
contain full miRNA precursor sequences with constitutive promoter.
Vectors with human (hsa) sequences contain a puromycin
selection marker
Vectors with mouse (mmu)
sequences contain a GFPpuromycin fusion to allow selection by either marker

1. Mallory, M.J. et al. (2011). Signal and developmental-dependent alternative splicing
of LEF1 in T cells is controlled by CELF2.
Mol. Cell. Biol. 31:2184-2195. (MIR-23B)
2. Saus, E. et al. (2010). Genetic variants and
abnormal processing of pre-miR-182, a
circadian clock modulator, in major depression patients with late insomnia. Hum. Mol.
Genet. 19:4017. (MIR-182)
3. Majid, S. et al. (2011). MicroRNA-205 inhibits src-mediated oncogenic pathways in
renal cancer. Cancer Res. 71:2611-2621.
(MIR-205)
Dont see your miRNA of interest? Clone your own sequence

into one of our Expression Vectorssee page 72.
Precursor Name
Precursor Name
Catalog Number
mmu-mir-20a
hsa-mir-20b
mmu-mir-20b
hsa-mir-21
mmu-mir-21
MMU-MIR-20A
MIR-20B
MMU-MIR-20B
MIR-21
MMU-MIR-21
MIR-22
hsa-mir-23b
MIR-23B
mmu-mir-23b
MMU-MIR-23B
mmu-mir-24-1
MMU-MIR-24-1
hsa-mir-24-2
MMU-LET7A-1
mmu-mir-7b
MMU-MIR-7B
hsa-let-7a-2
MIR-LET7A-2
hsa-mir-9-1
MIR-9-1
hsa-let-7a-3
MIR-LET7A-3
mmu-mir-9-1
mmu-let-7b
MMU-LET7B
hsa-mir-9-2
MIR-9-2
mmu-mir-26a-1
hsa-let7c
MIR-LET7C
hsa-mir-10a
MIR-10A
hsa-mir-26a-2
mmu-let-7c-1
MMU-LET7C-1
hsa-mir-10b
MIR-10B
mmu-mir-26a-2
mmu-let-7c-2
MMU-LET7C-2
mmu-mir-10b
MMU-MIR-9-1
Catalog Number
hsa-mir-22
mmu-let-7a-1
mmu-mir-24-2
mmu-mir-25
hsa-mir-26a-1
MIR-24-2
MMU-MIR-24-2
MMU-MIR-25
MIR-26A-1
MMU-MIR-26A-1
MIR-26A-2
MMU-MIR-26A-2
MMU-MIR-10B
hsa-mir-26b
MIR-26B
MIR-15A
hsa-mir-27a
MIR-27A
MIR-27B
hsa-let-7d
MIR-LET7D
hsa-mir-15a
mmu-let-7d
MMU-LET7D
mmu-mir-15a
MMU-MIR-15A
hsa-mir-27b
MIR-LET7E
mmu-mir-15b
MMU-MIR-15B
mmu-mir-27b
hsa-let-7f-1
MIR-LET7F-1
hsa-mir-16-1
MIR-16-1
mmu-let-7f-1
MMU-LET7F-1
mmu-mir-16-1
mmu-let-7f-2
MMU-LET7F-2
hsa-mir-16-2
hsa-let-7e
hsa-mir-28
MMU-MIR-27B
MIR-28
MMU-MIR-16-1
mmu-mir-28
MMU-MIR-28
MIR-16-2
hsa-mir-29a
MIR-29A
hsa-let-7g
MIR-LET7G
hsa-mir-17
MIR-17
mmu-mir-29a
MMU-MIR-29A
mmu-let-7g
MMU-LET7G
hsa-mir-18a
MIR-18A
hsa-mir-29b-1
MIR-29B-1
hsa-let-7i
hsa-mir-1-1
mmu-mir-1-1
hsa-mir-1-2
mmu-mir-1-2
66
Catalog Number
Precursor Name
MIR-LET7I
MIR-1-1
MMU-MIR-1-1
MIR-1-2
mmu-mir-18a
hsa-mir-18b
mmu-mir-18b
hsa-mir-19a
MMU-MIR-18A
mmu-mir-29b-1
MMU-MIR-29B-1
MIR-18B
mmu-mir-29b-2
MMU-MIR-29B-2
MMU-MIR-18B
mmu-mir-29c
MMU-MIR-29C
hsa-mir-30a
MIR-30A
mmu-mir-19a
MMU-MIR-19A
hsa-mir-7-1
MIR-7-1
hsa-mir-19b-1
MIR-19B-1
hsa-mir-7-2
MIR-7-2
mmu-mir-19b-1
hsa-mir-7-3
MIR-7-3
hsa-mir-19b-2
MMU-MIR-7A-1
mmu-mir-19b-2
mmu-mir-7a-2
MMU-MIR-7A-2
hsa-mir-20a
Phone 1-858-271-6500
MIR-29C
MIR-19A
MMU-MIR-1-2
mmu-mir-7a-1
hsa-mir-29c
MMU-MIR-19B-1
MIR-19B-2
MMU-MIR-19B-2
MIR-20A
mmu-mir-30a
hsa-mir-30b
hsa-mir-30c-1
mmu-mir-30c-1
hsa-mir-30c-2
MMU-MIR-30A
MIR-30B
MIR-30C-1
MMU-MIR-30C-1
MIR-30C-2
Fax 1-858-271-6514
MICRORNA
ANALYSIS
miRNASelect Precursor Clone Collection (Expression Vectors), cont.

Precursor Name
Catalog Number
Precursor Name
mmu-mir-30c-2
MMU-MIR-30C-2
hsa-mir-106b
hsa-mir-30d
mmu-mir-30d
hsa-mir-30e
mmu-mir-30e
hsa-mir-31
mmu-mir-31
MIR-30D
MMU-MIR-30D
mmu-mir-106b
hsa-mir-107
Catalog Number
Precursor Name
Catalog Number
MIR-106B
mmu-mir-135a-2
MMU-MIR-135A-2
MMU-MIR-106B
MIR-107
hsa-mir-135b
mmu-mir-135b
MIR-30E
mmu-mir-107
MMU-MIR-107
hsa-mir-136
MMU-MIR-30E
mmu-mir-122
MMU-MIR-122
mmu-mir-136
MIR-31
MMU-MIR-31
mmu-mir-124-1
hsa-mir-124-2
MMU-MIR-124-1
hsa-mir-137
MIR-136
MMU-MIR-136
MIR-137
mmu-mir-137
MMU-MIR-137
MIR-138-1
mmu-mir-124-2
MMU-MIR-124-2
hsa-mir-138-1
mmu-mir-32
MMU-MIR-32
mmu-mir-124-3
MMU-MIR-124-3
mmu-mir-138-1
hsa-mir-33a
MIR-33A
mmu-mir-125a
MMU-MIR-125A
hsa-mir-138-2
hsa-mir-34c
MIR-34C
hsa-mir-125b-2
MIR-125B-2
mmu-mir-125b-2
MMU-MIR-135B
MIR-124-2
MIR-32
hsa-mir-32
MIR-135B
MMU-MIR-125B-2
mmu-mir-138-2
MIR-138-2
MMU-MIR-138-2
mmu-mir-34c
MMU-MIR-34C
hsa-mir-92a-1
MIR-92A-1
mmu-mir-126
MMU-MIR-126
mmu-mir-139
MMU-MIR-92A-1
mmu-mir-127
MMU-MIR-127
hsa-mir-140
MIR-92A-2
hsa-mir-128-1
MIR-128-1
mmu-mir-140
MMU-MIR-140
MMU-MIR-128-1
mmu-mir-141
MMU-MIR-141
mmu-mir-92a-1
hsa-mir-92a-2
mmu-mir-92a-2
MMU-MIR-92A-2
mmu-mir-92b
MMU-MIR-92B
mmu-mir-93
MMU-MIR-93
mmu-mir-128-1
hsa-mir-128-2
mmu-mir-128-2
hsa-mir-95
MIR-95
hsa-mir-129-1
hsa-mir-96
MIR-96
mmu-mir-129-1
mmu-mir-96
hsa-mir-98
MMU-MIR-96
MIR-98
mmu-mir-98
MMU-MIR-98
hsa-mir-100
MIR-100
hsa-mir-129-2
mmu-mir-129-2
hsa-mir-130a
mmu-mir-130a
hsa-mir-130b
mmu-mir-100
MMU-MIR-100
mmu-mir-101a
MMU-MIR-101A
mmu-mir-130b
mmu-mir-101b
MMU-MIR-101B
hsa-mir-132
hsa-mir-103-1
MIR-103-1
mmu-mir-132
MIR-128-2
MMU-MIR-128-2
MIR-129-1
hsa-mir-139
MMU-MIR-138-1
hsa-mir-142
mmu-mir-142
hsa-mir-143
MIR-139
MMU-MIR-139
MIR-140
MIR-142
MMU-MIR-142
MIR-143
MMU-MIR-129-1
mmu-mir-143
MMU-MIR-143
MIR-129-2
mmu-mir-144
MMU-MIR-144
MMU-MIR-129-2
hsa-mir-145
MIR-145
MIR-130A
mmu-mir-145
MMU-MIR-145
MMU-MIR-130A
mmu-mir-146a
MMU-MIR-146A
MIR-130B
hsa-mir-146b
MIR-146B
MMU-MIR-130B
hsa-mir-147
MIR-147
MIR-132
hsa-mir-147b
MIR-147B
MMU-MIR-132
hsa-mir-148a
MIR-148A
MMU-MIR-103-1
mmu-mir-133a-1
MMU-MIR-133A-1
mmu-mir-148a
MMU-MIR-148A
hsa-mir-103-2
MIR-103-2
mmu-mir-133a-2
MMU-MIR-133A-2
mmu-mir-149
MMU-MIR-149
mmu-mir-105
MMU-MIR-105
mmu-mir-133b
MMU-MIR-133B
hsa-mir-105-1
MIR-105-1
mmu-mir-134
MMU-MIR-134
hsa-mir-105-2
MIR-105-2
hsa-mir-135a-1
hsa-mir-106a
MIR-106A
mmu-mir-135a-1
mmu-mir-103-1
mmu-mir-106a
MMU-MIR-106A
hsa-mir-135a-2
MIR-135A-1
MMU-MIR-135A-1
MIR-135A-2
www.cellbiolabs.com
hsa-mir-150
mmu-mir-150
hsa-mir-151
mmu-mir-151
hsa-mir-152
MIR-150
MMU-MIR-150
MIR-151
MMU-MIR-151
MIR-152
67
MICRORNA
ANALYSIS

Precursor Name
Catalog Number
Precursor Name
Catalog Number
Precursor Name
mmu-mir-152
MMU-MIR-152
mmu-mir-196a-2
MMU-MIR-196A-2
mmu-mir-217
mmu-mir-153
MMU-MIR-153
mmu-mir-196b
hsa-mir-154
MMU-MIR-218-1
MMU-MIR-218-2
MIR-197
mmu-mir-218-2
mmu-mir-154
MMU-MIR-154
hsa-mir-198
MIR-198
hsa-mir-219-1
mmu-mir-155
MMU-MIR-155
hsa-mir-199a-1
mmu-mir-199a-1
mmu-mir-181a-1
MMU-MIR-181A-1
hsa-mir-199a-2
mmu-mir-181a-2
MMU-MIR-181A-2
hsa-mir-200a
mmu-mir-181b-1
MMU-MIR-181B-1
mmu-mir-181b-2
MMU-MIR-181B-2
mmu-mir-181c
MMU-MIR-181C
hsa-mir-181d
MIR-181D
mmu-mir-182
MIR-219-1
MIR-199A-1
mmu-mir-219-1
MMU-MIR-219-1
MMU-MIR-199A-1
mmu-mir-219-2
MMU-MIR-219-2
MIR-199A-2
hsa-mir-222
MIR-222
MIR-200A
mmu-mir-222
MMU-MIR-222
mmu-mir-200a
MMU-MIR-200A
mmu-mir-223
MMU-MIR-223
mmu-mir-200b
MMU-MIR-200B
hsa-mir-224
mmu-mir-224
MMU-MIR-224
mmu-mir-200c
MMU-MIR-200C
mmu-mir-290
MMU-MIR-290
MMU-MIR-182
mmu-mir-201
MMU-MIR-201
mmu-mir-291a
MMU-MIR-291A
mmu-mir-183
MMU-MIR-183
hsa-mir-202
MIR-202
mmu-mir-291b
MMU-MIR-291B
mmu-mir-184
MMU-MIR-184
mmu-mir-202
MMU-MIR-202
mmu-mir-292
MMU-MIR-292
MIR-204
mmu-mir-293
MMU-MIR-293
MMU-MIR-204
mmu-mir-294
MMU-MIR-294
MIR-205
mmu-mir-295
MMU-MIR-295
MMU-MIR-205
mmu-mir-296
MMU-MIR-296
mmu-mir-185
MIR-185
MMU-MIR-185
hsa-mir-200c
MIR-224
MIR-200C
hsa-mir-185
hsa-mir-204
mmu-mir-204
hsa-mir-186
MIR-186
hsa-mir-205
hsa-mir-187
MIR-187
mmu-mir-205
mmu-mir-187
MMU-MIR-187
hsa-mir-206
mmu-mir-188
MMU-MIR-188
mmu-mir-206
MMU-MIR-206
mmu-mir-297a-1
MMU-MIR-297A-1
MIR-190
hsa-mir-208a
MIR-208A
mmu-mir-297a-3
MMU-MIR-297A-3
hsa-mir-190
MIR-206
hsa-mir-297
MIR-297
mmu-mir-190
MMU-MIR-190
mmu-mir-208a
MMU-MIR-208A
mmu-mir-297a-4
MMU-MIR-297A-4
mmu-mir-190b
MMU-MIR-190B
mmu-mir-208b
MMU-MIR-208B
mmu-mir-297a-5
MMU-MIR-297A-5
mmu-mir-191
MMU-MIR-191
mmu-mir-210
MMU-MIR-210
mmu-mir-297a-6
MMU-MIR-297A-6
mmu-mir-192
MMU-MIR-192
hsa-mir-211
mmu-mir-193
MMU-MIR-193
mmu-mir-211
mmu-mir-193b
MMU-MIR-193B
hsa-mir-194-1
MIR-194-1
hsa-mir-212
mmu-mir-212
MIR-211
mmu-mir-297b
MMU-MIR-297B
MMU-MIR-211
mmu-mir-297c
MMU-MIR-297C
MIR-212
hsa-mir-298
MIR-298
MMU-MIR-212
mmu-mir-298
MMU-MIR-298
MIR-214
mmu-mir-299
MMU-MIR-299
mmu-mir-194-1
MMU-MIR-194-1
hsa-mir-214
mmu-mir-194-2
MMU-MIR-194-2
mmu-mir-214
MMU-MIR-214
hsa-mir-301a
MIR-301A
MIR-195
mmu-mir-215
MMU-MIR-215
hsa-mir-301b
MIR-301B
MIR-196A-1
hsa-mir-216a
MIR-216A
hsa-mir-302a
MIR-302A
hsa-mir-195
hsa-mir-196a-1
mmu-mir-196a-1
hsa-mir-196a-2
68
mmu-mir-218-1
hsa-mir-197
MIR-181A-1
MMU-MIR-217
MMU-MIR-196B
MIR-154
hsa-mir-181a-1
Catalog Number
MMU-MIR-196A-1
mmu-mir-216a
MMU-MIR-216A
mmu-mir-302a
MIR-196A-2
mmu-mir-216b
MMU-MIR-216B
hsa-mir-302b
Phone 1-858-271-6500
MMU-MIR-302A
MIR-302B
Fax 1-858-271-6514
MICRORNA
ANALYSIS

Precursor Name
Catalog Number
Precursor Name
Catalog Number
Precursor Name
Catalog Number
mmu-mir-302b
MMU-MIR-302B
mmu-mir-376a
MMU-MIR-376A
mmu-mir-465c-1
MMU-MIR-465C-1
MIR-302C
mmu-mir-376c
MMU-MIR-376C
mmu-mir-465c-2
MMU-MIR-465C-2
mmu-mir-302c
MMU-MIR-302C
mmu-mir-377
MMU-MIR-377
mmu-mir-466a
mmu-mir-302d
MMU-MIR-302D
mmu-mir-378
MMU-MIR-378
mmu-mir-466b-2
MMU-MIR-466B-2
mmu-mir-320
MMU-MIR-320
mmu-mir-379
MMU-MIR-379
mmu-mir-466b-3
MMU-MIR-466B-3
mmu-mir-322
MMU-MIR-322
mmu-mir-380
MMU-MIR-380
mmu-mir-466d
MIR-323
mmu-mir-381
MMU-MIR-381
mmu-mir-466f-1
MMU-MIR-466F-1
mmu-mir-324
MMU-MIR-324
mmu-mir-382
MMU-MIR-382
mmu-mir-466f-4
MMU-MIR-466F-4
mmu-mir-325
MMU-MIR-325
mmu-mir-383
MMU-MIR-383
mmu-mir-466g
MMU-MIR-466G
mmu-mir-328
MMU-MIR-328
mmu-mir-384
MMU-MIR-384
mmu-mir-466h
MMU-MIR-466H
hsa-mir-329-1
MIR-329-1
mmu-mir-409
MMU-MIR-409
mmu-mir-466i
MMU-MIR-466I
mmu-mir-330
MMU-MIR-330
mmu-mir-410
MMU-MIR-410
mmu-mir-466j
MMU-MIR-466J
MIR-331
mmu-mir-411
MMU-MIR-411
mmu-mir-466k
MMU-MIR-466K
MMU-MIR-331
mmu-mir-412
MMU-MIR-412
mmu-mir-466l
MMU-MIR-466L
MIR-335
mmu-mir-421
MMU-MIR-421
mmu-mir-467b
MMU-MIR-467B
mmu-mir-337
MMU-MIR-337
mmu-mir-423
MMU-MIR-423
mmu-mir-467d
MMU-MIR-467D
mmu-mir-338
MMU-MIR-338
mmu-mir-425
MMU-MIR-425
mmu-mir-467e
MMU-MIR-467E
mmu-mir-341
MMU-MIR-341
mmu-mir-429
MMU-MIR-429
mmu-mir-467f
MMU-MIR-467F
MIR-342
mmu-mir-431
MMU-MIR-431
mmu-mir-467g
MMU-MIR-467G
MIR-433
mmu-mir-469
MMU-MIR-469
hsa-mir-302c
hsa-mir-323
hsa-mir-331
mmu-mir-331
hsa-mir-335
hsa-mir-342
MMU-MIR-466A
MMU-MIR-466D
mmu-mir-342
MMU-MIR-342
hsa-mir-433
mmu-mir-343
MMU-MIR-343
mmu-mir-433
MMU-MIR-433
mmu-mir-483
MMU-MIR-483
mmu-mir-344-1
MMU-MIR-344-1
mmu-mir-434
MMU-MIR-434
mmu-mir-484
MMU-MIR-484
mmu-mir-344-2
MMU-MIR-344-2
mmu-mir-448
MMU-MIR-448
mmu-mir-485
MMU-MIR-485
mmu-mir-345
MMU-MIR-345
mmu-mir-449a
MMU-MIR-449A
mmu-mir-486
MMU-MIR-486
mmu-mir-346
MMU-MIR-346
mmu-mir-449b
MMU-MIR-449B
mmu-mir-487b
MMU-MIR-487B
mmu-mir-351
MMU-MIR-351
mmu-mir-449c
MMU-MIR-449C
mmu-mir-488
MMU-MIR-488
mmu-mir-361
MMU-MIR-361
mmu-mir-450a-2
MMU-MIR-450A-2
mmu-mir-489
MMU-MIR-489
mmu-mir-363
MMU-MIR-363
hsa-mir-450b
MIR-450B
mmu-mir-490
MMU-MIR-490
mmu-mir-365-1
MMU-MIR-365-1
mmu-mir-451
MMU-MIR-451
hsa-mir-491
mmu-mir-365-2
MMU-MIR-365-2
mmu-mir-452
MMU-MIR-452
mmu-mir-491
MMU-MIR-491
mmu-mir-367
MMU-MIR-367
mmu-mir-455
MMU-MIR-455
mmu-mir-493
MMU-MIR-493
mmu-mir-370
MMU-MIR-370
mmu-mir-464
MMU-MIR-464
mmu-mir-494
MMU-MIR-494
mmu-mir-374
MMU-MIR-374
mmu-mir-465a
MMU-MIR-465A
mmu-mir-495
MMU-MIR-495
hsa-mir-374b
MIR-374B
mmu-mir-465b-1
MMU-MIR-465B-1
mmu-mir-496
MMU-MIR-496
mmu-mir-375
MMU-MIR-375
mmu-mir-465b-2
MMU-MIR-465B-2
mmu-mir-497
MMU-MIR-497
www.cellbiolabs.com
MIR-491
69
MICRORNA
ANALYSIS

Precursor Name
hsa-mir-499
MIR-499
Precursor Name
hsa-mir-568
Catalog Number
Product Name
Catalog Number
MIR-568
hsa-mir-633
MIR-633
MMU-MIR-568
hsa-mir-635
MIR-635
mmu-mir-499
MMU-MIR-499
mmu-mir-568
mmu-mir-501
MMU-MIR-501
hsa-mir-569
MIR-569
hsa-mir-636
MIR-636
MIR-503
hsa-mir-577
MIR-577
hsa-mir-637
MIR-637
MMU-MIR-503
hsa-mir-579
MIR-579
hsa-mir-641
MIR-641
MIR-504
hsa-mir-580
MIR-580
hsa-mir-643
MIR-643
MMU-MIR-504
hsa-mir-581
MIR-581
hsa-mir-645
MIR-645
MIR-505
hsa-mir-582
MIR-582
hsa-mir-649
MIR-649
MMU-MIR-582
hsa-mir-651
MIR-651
MIR-652
hsa-mir-503
mmu-mir-503
hsa-mir-504
mmu-mir-504
hsa-mir-505
mmu-mir-505
MMU-MIR-505
mmu-mir-582
hsa-mir-506
MIR-506
hsa-mir-584
MIR-584
hsa-mir-652
hsa-mir-508
MIR-508
hsa-mir-585
MIR-585
mmu-mir-652
hsa-mir-509-1
MIR-509-1
hsa-mir-591
MIR-591
hsa-mir-653
hsa-mir-514-1
MIR-514-1
hsa-mir-592
MIR-592
mmu-mir-653
hsa-mir-514-2
MIR-514-2
mmu-mir-592
hsa-mir-520f
MIR-520F
hsa-mir-525
MMU-MIR-652
MIR-653
MMU-MIR-653
MMU-MIR-592
hsa-mir-654
MIR-654
hsa-mir-595
MIR-595
hsa-mir-658
MIR-658
MIR-525
hsa-mir-596
MIR-596
hsa-mir-659
MIR-659
MIR-526A-2
hsa-mir-597
MIR-597
hsa-mir-665
MIR-665
mmu-mir-539
MMU-MIR-539
hsa-mir-598
MIR-598
mmu-mir-665
MMU-MIR-665
mmu-mir-540
MMU-MIR-540
mmu-mir-598
MMU-MIR-598
mmu-mir-666
MMU-MIR-666
hsa-mir-526a-2
MIR-541
hsa-mir-599
MIR-599
mmu-mir-667
MMU-MIR-667
MMU-MIR-541
hsa-mir-600
MIR-600
mmu-mir-668
MMU-MIR-668
MIR-542
hsa-mir-601
MIR-601
mmu-mir-669a-3
MMU-MIR-542
hsa-mir-602
MIR-602
mmu-mir-669b
MMU-MIR-669B
MIR-543
hsa-mir-603
MIR-603
mmu-mir-669c
MMU-MIR-669C
mmu-mir-546
MMU-MIR-546
hsa-mir-605
MIR-605
mmu-mir-669d
MMU-MIR-669D
mmu-mir-547
MMU-MIR-547
hsa-mir-606
MIR-606
mmu-mir-669e
MMU-MIR-669E
hsa-mir-548a-1
MIR-548A-1
hsa-mir-608
MIR-608
mmu-mir-669g
MMU-MIR-669G
hsa-mir-548a-3
MIR-548A-3
hsa-mir-609
MIR-609
mmu-mir-669h
MMU-MIR-669H
hsa-mir-548b
MIR-548B
hsa-mir-610
MIR-610
mmu-mir-669j
MMU-MIR-669J
hsa-mir-548c
MIR-548C
hsa-mir-613
MIR-613
mmu-mir-669k
MMU-MIR-669K
hsa-mir-548d-1
MIR-548D-1
hsa-mir-616
MIR-616
mmu-mir-670
MMU-MIR-670
hsa-mir-548d-2
MIR-548D-2
hsa-mir-619
MIR-619
hsa-mir-671
hsa-mir-551b
MIR-551B
hsa-mir-620
MIR-620
mmu-mir-672
MMU-MIR-672
hsa-mir-554
MIR-554
hsa-mir-628
MIR-628
mmu-mir-674
MMU-MIR-674
hsa-mir-555
MIR-555
hsa-mir-630
MIR-630
mmu-mir-675
MMU-MIR-675
hsa-mir-541
mmu-mir-541
hsa-mir-542
mmu-mir-542
hsa-mir-543
70
Catalog Number
Phone 1-858-271-6500
MMU-MIR-669A-3
MIR-671
Fax 1-858-271-6514
MICRORNA
ANALYSIS

Product Name
Catalog Number
Product Name
Catalog Number
Product Name
Catalog Number
MMU-MIR-744
hsa-mir-921
MIR-921
MIR-758
hsa-mir-922
MIR-922
mmu-mir-758
MMU-MIR-758
hsa-mir-923
MIR-923
MMU-MIR-681
mmu-mir-759
MMU-MIR-759
hsa-mir-924
MIR-924
MMU-MIR-682
mmu-mir-761
MMU-MIR-761
hsa-mir-933
MIR-933
mmu-mir-684-1
MMU-MIR-684-1
mmu-mir-763
MMU-MIR-763
hsa-mir-934
MIR-934
mmu-mir-684-2
MMU-MIR-684-2
mmu-mir-764
MMU-MIR-764
hsa-mir-935
MIR-935
mmu-mir-676
MMU-MIR-676
mmu-mir-744
mmu-mir-677
MMU-MIR-677
hsa-mir-758
mmu-mir-679
MMU-MIR-679
mmu-mir-681
mmu-mir-682
mmu-mir-686
MMU-MIR-686
hsa-mir-766
MIR-766
hsa-mir-936
MIR-936
mmu-mir-688
MMU-MIR-688
hsa-mir-767
MIR-767
hsa-mir-937
MIR-937
mmu-mir-690
MMU-MIR-690
hsa-mir-770
MIR-770
hsa-mir-938
MIR-938
mmu-mir-694
MMU-MIR-694
mmu-mir-770
MMU-MIR-770
hsa-mir-940
MIR-940
mmu-mir-695
MMU-MIR-695
hsa-mir-802
MIR-802
hsa-mir-941
MIR-941
mmu-mir-697
MMU-MIR-697
mmu-mir-802
MMU-MIR-802
hsa-mir-942
MIR-942
mmu-mir-698
MMU-MIR-698
mmu-mir-804
MMU-MIR-804
mmu-mir-1187
MMU-MIR-1187
mmu-mir-699
MMU-MIR-699
mmu-mir-871
MMU-MIR-871
mmu-mir-1188
MMU-MIR-1188
mmu-mir-700
MMU-MIR-700
mmu-mir-872
MMU-MIR-872
mmu-mir-1191
MMU-MIR-1191
mmu-mir-701
MMU-MIR-701
mmu-mir-873
MMU-MIR-873
mmu-mir-1192
MMU-MIR-1192
mmu-mir-702
MMU-MIR-702
hsa-mir-874
MIR-874
mmu-mir-1193
MMU-MIR-1193
mmu-mir-703
MMU-MIR-703
mmu-mir-874
MMU-MIR-874
mmu-mir-1195
MMU-MIR-1195
mmu-mir-704
MMU-MIR-704
mmu-mir-875
MMU-MIR-875
mmu-mir-1197
MMU-MIR-1197
mmu-mir-705
MMU-MIR-705
hsa-mir-876
MIR-876
mmu-mir-1198
MMU-MIR-1198
MMU-MIR-876
mmu-mir-1224
MMU-MIR-1224
MIR-877
mmu-mir-1892
MMU-MIR-1892
hsa-mir-708
MIR-708
mmu-mir-876
mmu-mir-708
MMU-MIR-708
hsa-mir-877
mmu-mir-711
MMU-MIR-711
mmu-mir-877
MMU-MIR-877
mmu-mir-1894
MMU-MIR-1894
mmu-mir-713
MMU-MIR-713
mmu-mir-878
MMU-MIR-878
mmu-mir-1895
MMU-MIR-1895
mmu-mir-715
MMU-MIR-715
mmu-mir-879
MMU-MIR-879
mmu-mir-1896
MMU-MIR-1896
mmu-mir-717
MMU-MIR-717
mmu-mir-880
MMU-MIR-880
mmu-mir-1897
MMU-MIR-1897
mmu-mir-719
MMU-MIR-719
mmu-mir-881
MMU-MIR-881
mmu-mir-1898
MMU-MIR-1898
mmu-mir-720
MMU-MIR-720
mmu-mir-883A
MMU-MIR-883A
mmu-mir-1899
MMU-MIR-1899
mmu-mir-721
MMU-MIR-721
mmu-mir-883B
MMU-MIR-883B
mmu-mir-1900
MMU-MIR-1900
mmu-mir-741
MMU-MIR-741
hsa-mir-885
MIR-885
mmu-mir-1902
MMU-MIR-1902
mmu-mir-742
MMU-MIR-742
hsa-mir-889
MIR-889
mmu-mir-1903
MMU-MIR-1903
mmu-mir-743a
MMU-MIR-743A
hsa-mir-891a
MIR-891A
mmu-mir-1904
MMU-MIR-1904
mmu-mir-743b
MMU-MIR-743B
hsa-mir-892b
MIR-892B
mmu-mir-1905
MMU-MIR-1905
MIR-744
hsa-mir-920
MIR-920
mmu-mir-1907
MMU-MIR-1907
hsa-mir-744
www.cellbiolabs.com
71
MICRORNA
ANALYSIS
Expression, Control, Reporter Vectors
miRNASelect Expression and Control Vectors

Our miRNASelect Mammalian Expression Vectors
provide an easy, efficient method to clone a miRNA
precursor from any species. The desired miRNA sequence is cloned into a human -globin intron contained within the vector. Two vector formats are available:
The pEP vector contains a puromycin selection

marker
The pEGP vector contains a GFP-puromycin fusion to allow selection by either marker
Each expression vector is provided with a null

(empty) control vector at no extra charge. Null control
vectors are also sold separately for use with vectors
from our miRNASelect Precursor Clone Collection.

Dar, A. et al. (2010). miRNA-205 suppresses melanoma cell proliferation and induces senescence via regulation of E2F1 protein. J.
Biol. Chem. 286:16606-16614. (MIR-NULL)
Product Name
Size
Catalog Number
miRNASelect pEGP-mir Cloning and Expression Vector
100 L
MIR-EXP-GP-C
miRNASelect pEP-mir Cloning and Expression Vector
100 L
MIR-EXP-C
miRNASelect pEGP-mir Null Control Vector
100 L
MIR-NULL-GP
miRNASelect pEP-mir Null Control Vector
100 L
MIR-NULL
miRNASelect Functional Analysis Reporter System

The miRNASelect Functional Analysis Reporter
System provides a simple method for the evaluation of potential targets of miRNA. Binding of
miRNA sequences to their suspected targets results in repression of translation.
In this system the miRNA target sequence, such
as a 3 UTR, is cloned into the provided pMIRGFP vector in the multiple cloning sites immediately downstream of the GFP gene. If the miRNA
is present and binds to the target sequence, translation is repressed and no green fluorescence appears. If the miRNA does not bind the target sequence, GFP translation occurs normally and
green fluorescence may be seen.
72
Assay Principle.
If miRNA is present and binds to
the 3 UTR, translation of the GFP
gene is repressed,
resulting in loss of
fluorescence.
Product Name
Size
Catalog Number
miRNASelect pMIR-GFP Reporter System
1 Kit
MIR-GFP
Phone 1-858-271-6500
Fax 1-858-271-6514
MICRORNA
ANALYSIS
ViraSafe miRNA Lentivirus Expression System

Our ViraSafe Lentiviral Expression Systems provide a much safer method even compared to thirdgeneration lentivirus systems. Sequence homology
has been reduced an additional 80-90%, thereby substantially reducing the risk of replication-competent
lentivirus. Additionally, each vector is provided separately to allow for optimization of vector ratios.
For more information on our complete selection of ViraSafe Lentivirus Expression

Systems for gene expression studies,
please see p. 49-50.
The ViraSafe miRNA Lentivirus Expression System

is specifically designed to express microRNA in your
target cell.
Safer: 80-90% less sequence homology than
3rd-generation lentiviral expression systems;
ecotropic system provides even more safety*
High Titer: Incorporates elements that provide
titers comparable to 3rd-generation systems
Flexible: Vectors provided separately for increased safety and to allow optimization of vector ratios
*Lentiviruses made with a ViraSafe Ecotropic Expression System will only readily infect
mouse and rat cells. Pantropic lentiviruses are VSVG-pseudotyped and may infect cells of
any species.
Product Name
Envelope
Size
Catalog Number
Ecotropic
1 Kit
VPK-220-ECO
Pantropic (VSVG)
1 Kit
VPK-220-PAN
N/A
10 g
VPK-220
ViraSafe miRNA Lentiviral Expression System

pSMPUW-miR-GFP-Puro Lentiviral Expression Vector

RAPAd Adenoviral Expression Systems produce
recombinant adenovirus with substantial reduction
in wild-type adenovirus, while doing so in a much
shorter 2 week time frame. The systems use a
backbone vector from which the 5 ITR, packaging
signal and E1 sequences have been removed.
Additionally, serial amplification of the recombinant
adenovirus does not increase the level of replication-competent adenovirus.
The RAPAd miRNA Adenoviral Expression System

is specifically designed to deliver miRNA sequences
into your target cell.
Virtually No Wild-Type Virus: Backbone vector
engineered to produce <300 wild-type plaques per
109 particles, compared with 104-106 WT plaques
per 109 VP with most other methods
Faster Virus Production: Virus generated in 2
weeks compared to a few months with traditional
methods
For more information on our complete selection of RAPAd Adenovirus Expression

Systems for gene expression studies, please see page 41.
Product Name
Size
Catalog Number
1 Kit
VPK-253
www.cellbiolabs.com
73
MICRORNA
ANALYSIS
Viral Vectors, Knockdown Enhancer
miRNA Retroviral Expression Vector

Our pMXs-miR-GFP/Puro Retroviral Vector allows
you to clone a miRNA sequence of interest for packaging into a recombinant retrovirus for delivery into a
target cell.
For efficient packaging of your miRNA into

an MMLV-based retrovirus, use one of our
Platinum Retroviral Packaging Cell Lines
found on page 61.
Product Name
Size
Catalog Number
pMXs-miR-GFP/Puro Retroviral Vector
10 g
RTV-017
RNAiBoost Reagent Kit for miRNA and siRNA enhancement

RNA interference can occur in the presence of either siRNA or the mature form of miRNA. The RNABoost
Reagent Kit increases the level of interference in the presence of siRNA. It also increases the rate of processing
of pre-miRNA into mature miRNA.
Product Name
Size
Catalog Number
20 reactions
RNAI-200
100 reactions
RNAI-201
RNAiBoost Reagent Kit
74
Phone 1-858-271-6500
Fax 1-858-271-6514
OXIDATIVE STRESS / DAMAGE
Oxidative Stress Overview
Measuring Oxidative Stress

Oxidative stress may be measured using one of three primary methods:
Measure the reactive oxygen species (ROS) directly

Measure the presence of antioxidants
Measure the resulting damage to proteins, lipids, DNA or RNA (most reliable)
Use the following table to determine the best oxidative stress assays for your samples.
Marker or
Type of Damage
Oxidative
Protein Damage
(p. 77-81)
Lipid Peroxidation
(p. 82-85)
Sample Type
Cells Tissues
Protein carbonyl content (PCC)
3-Nitrotyrosine
BPDE Protein Adduct
Advanced Glycation End Products (AGE)
Advanced Oxidation Protein Products (AOPP)
4-Hydroxynonenal (4-HNE)
Malondialdehyde (MDA)
8-iso-Prostaglandin F2 (8-Isoprostane)
Other
8-hydroxyguanosine (8-OHG)
8-hydroxydeoxyguanosine (8-OHdG)
Abasic (AP) sites
BPDE DNA Adduct
Double-strand DNA breaks
UV DNA Damage (CPD and 6-4PP)
Comet Assay
Superoxide Dismutase
Catalase
Glutathione
Total Antioxidant Capacity (TAC)
Oxygen Radical Antioxidant Capacity (ORAC)
Food
Hydroxyl Radical Antioxidant Capacity (HORAC)
Food
Universal ROS (intracellular or in vitro)
Hydrogen Peroxide
DNA / RNA Damage

and Repair
(p. 86-91)
Antioxidants
& Antioxidant
Capacity
(p. 94-96)
76
Urine
Kidney Injury Molecule-1 (KIM-1)
Oxidized Low Density Lipoprotein (OxLDL)
Reactive Oxygen
Species
(p. 92-93)
Blood
Phone 1-858-271-6500
CS fluid
Food
Fax 1-858-271-6514
Protein Oxidation
Assays and Reagents for Oxidative Protein Damage

Cellular proteins are subject to damage in the presence of reactive oxygen species (ROS).
The resulting protein damage may take the form of nitration or oxidation of various amino
acid residues, or may result in formation of advanced glycation end products (AGE) or advanced oxidation protein products (AOPP). We have developed unique assays to detect
protein damage with higher sensitivity and more user-friendly protocols.
OxiSelect Nitrotyrosine Assay Kits and Antibodies

Our OxiSelect Nitrotyrosine Assay Kits provide a
simple method to measure the formation of 3nitrotyrosine in proteins. This assay is available in two
formats: a 96-well competitive ELISA and an immunoblot kit. The ELISA format can detect the presence of 3-nitrotyrosine as low as 10 nM.

1. Drel, V.R. et al. (2010). New therapeutic and biomarker discovery for peripheral diabetic retinopathy: PARP inhibitor, nitrotyrosine, and tumor necrosis factor-alpha. Endocrinology 151:2547
-2555. (STA-305)
2. Cheah, F.-C. et al. (2009). Airway inflammatory cell responses
to intra-amniotic lipopolysaccharide in a sheep model of
chorioamnionitis. Am. J. Physiol. Lung Cell Mol. Physiol.
296:L384-L393. (STA-305)
3. Drel, V.R. et al. (2009). Poly(adenosine 5'-diphosphate-ribose)
polymerase inhibition counteracts multiple manifestations of
experimental type 1 diabetic nephropathy. Endocrinology
150:5273-5283. (STA-305)
4. Li, X. et al. (2008). Lipoamide protects retinal pigment epithelial
cells from oxidative stress and mitochondrial dysfunction. Free
Radic. Biol. Med. 44(7):1465-1474. (STA-305)
OD 450 nm
1.6
1.2
0.8
0.4
0
Control
Formation of 3-Nitrotyrosine During Oxidative Stress.

Product Name
TNM
Protein Nitration by Tetranitromethane using the OxiSelect

Nitrotyrosine ELISA Kit. STO (MEF) cells were lysed and nitrated
with tetranitromethane. The protein 3-nitrotyrosine levels were
measured as described in the assay protocol.
Size
Catalog Number
96 Assays
STA-305
5 x 96 Assays
STA-305-5
Immunoblot/ECL
10 Blots
STA-303
Goat Anti-Nitrotyrosine Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-003
Rabbit Anti-Nitrotyrosine Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-004
Immunoblot/ECL
10 g
STA-304
Nitrotyrosine ELISA Kit

Nitrotyrosine Immunoblot Kit
Protein Tyrosine Nitration Control (Nitrotyrosine-BSA)
Detection
Colorimetric
www.cellbiolabs.com
77
Protein Oxidation
OxiSelect Protein Carbonyl Assay Kits

The most common products of protein oxidation in
biological samples are the carbonyl derivatives of
Pro, Arg, Lys and Thr residues. Such derivatives are
chemically stable and serve as markers for oxidative
stress in most types of reactive oxygen species.
Our OxiSelect Protein Carbonyl Assay Kits provide rapid, efficient methods for detection of protein
carbonyls. Four assay formats are available: immunoblot, ELISA, fluorometric and spectrophotometric. All formats are suitable for use with purified protein, plasma, serum, or cell lysate samples.
Protein Carbonyl ELISA Kit
Sensitive: Detects samples as low as 10
g/ml
Greater Sample Retention: No concentration
or TCA precipitation steps that contribute to
sample loss
Protein Carbonyl Immunoblot Kit
No Molecular Weight Shift: DNPH derivatization
after immunoblotting allows direct comparison of
oxidized and non-oxidized protein fingerprints
2
1.75

1. Maity, P. et al. (2008). Indomethacin, a non-steroidal antiinflammatory drug, develops gastropathy by inducing reactive
oxygen species-mediated mitochondrial pathology and associated
apoptosis in gastric mucosa: A novel role of mitochondrial aconitase oxidation. J. Biol. Chem. 284:3058-3068. (STA-308)
2. Jia, L. et al (2007). Acrolein, a toxicant in cigarette smoke, causes
oxidative damage and mitochondrial dysfunction in RPE cells:
protection by (R)-alpha-lipoic acid. Invest. Ophthalmol. Vis. Sci.
48:339-348. (STA-308)
3. Liu, Z. et al. (2007). Hydroxytyrosol protects retinal pigment
epithelial cells from acrolein-induced oxidative stress and mitochondrial dysfunction. J. Neurochem. 103:2690-2700. (STA-308)
4. Ungvari, Z. et al. (2010). Extreme longevity is associated with
increased resistance to oxidative stress in Arctica islandica, the
longest living non-colonial animal. J. Gerontol. A. Biol. Sci. Med.
Sci. 66:741-750. (STA-310)
5. Labbe, A. et al. (2010). Resveratrol improves insulin resistance
hyperglycemia and hepatosteatosis but not hypertriglyercidemia,
inflammation, and life span in a mouse model for Werner syndrome. J. Gerontol. A. Biol. Sci. Med. Sci. 66:741-750. (STA-310)
6. Nolin, T.D. et al. (2010). Multiple-dose pharmacokinetics and
pharmacodynamics of N-acetylcysteine in patients with end-stage
renal disease. Clin. J. Am. Soc. Nephrol. 5:1588-1594. (STA-310)
7. Kang, K.A. et al. (2010). KIOM-4 protects against oxidative stress
-induced mitochondrial damage in pancreatic -cells via its antioxidant effects. Evid. Based Complement. Altern. Med. 10.1093/
ecam/neq007. (STA-310)
8. Cho, H-Y. et al. (2009). Antiviral activity of Nrf2 in a murine model
of respiratory syncytial virus (RSV) disease. Am. J. Respir. Crit.
Care Med. 179:138-150. (STA-310)
9. Neretti, N. et al. (2009). Long lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage. PNAS 106:2277-2282. (STA-310)
OD 450nm
1.5
1.25
1
0.75
0.5
0.25
0
0
Protein Carbonyl (nmol/mg)

Standard Curve Generated with the OxiSelect Protein Carbonyl ELISA Kit (STA-310).
Product Name
Detection
Size
Catalog Number
96 Assays
STA-310
5 x 96 Assays
STA-310-5
Fluorometric
100 Assays
STA-307
Spectrophotometric
40 Assays
STA-315
OxiSelect Protein Carbonyl Immunoblot Kit
Immunoblot/ECL
10 Blots
STA-308
Oxidized Protein Immunoblot Control (Carbonyl-BSA)
Immunoblot/ECL
10 g
STA-309
OxiSelect Protein Carbonyl ELISA Kit
Colorimetric
OxiSelect Protein Carbonyl Fluorometric Assay

OxiSelect Protein Carbonyl Spectrophotometric Assay
78
Assay Principle for the OxiSelect Protein Oxidation

Immunoblot Kit (STA-308).
Phone 1-858-271-6500
Fax 1-858-271-6514
Protein Oxidation
OxiSelect Advanced Glycation End Product (AGE) Kits & Antibodies

Advanced glycation end products are formed during
the Maillard reaction where reducing carbohydrates
react with lysine side chains and N-terminal amino
groups of various macromolecules, particularly proteins. These AGE products can adversely affect the
function of the affected proteins and play a role in
atherosclerosis, diabetes, aging and renal disease.
Our OxiSelect AGE ELISA Kit is designed for the
rapid detection of generic advanced glycation end
product protein adducts. For more specific detection,
we offer assays for N-epsilon-carboxymethyl lysine
(CML), N-epsilon-carboxyethyl lysine (CEL), and
methylglyoxal (MG).
Sensitive: Detect levels of CML as low as 35
pg/mL, CEL as low as 50 ng/mL, or MG as low
as 10 ng/mL
Versatile: Compatible with cell lysates, blood
samples, or purified proteins
Advanced Glycation End Products (AGE) Pathways.
3
Serum
Plasma
1.4
2.5
1.2
OD 450nm
OD 450 nm
0.8
1.5
0.6
0.4
0.5
0.2
0
0
0
Undiluted *
AGE-BSA (g/mL)
Standard Curve Generated with AGE-BSA Provided in the

OxiSelect AGE ELISA Kit.
Product Name
1:10 Dilution
CML Levels in Human Serum and Plasma as Measured by the

OxiSelect CML ELISA Kit.
Detection
Size
Catalog Number
OxiSelect Advanced Glycation End Product (AGE) ELISA Kit
Colorimetric
96 Assays
STA-317
OxiSelect Methylglyoxal (MG) ELISA Kit
Colorimetric
96 Assays
STA-311
OxiSelect N-(Carboxyethyl) Lysine (CEL) ELISA Kit
Colorimetric
96 Assays
STA-300
OxiSelect N-(Carboxymethyl) Lysine (CML) ELISA Kit
Colorimetric
96 Assays
STA-316
Immunoblot
10 Blots
STA-313
Immunoblot/
Immunohistochemistry
100 g
STA-011
Goat Anti-N-CML Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-013
Rabbit Anti-N-CML Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-014
N/A
100 g
STA-314
OxiSelect N -(Carboxymethyl) Lysine (CML) Immunoblot Kit

Mouse Anti-Methylglyoxal Monoclonal Antibody
CML-BSA Control
www.cellbiolabs.com
79
Protein Oxidation
OxiSelect AOPP Assay Kit

Fast: Obtain results in <30 minutes
Sensitive: Detect concentrations as low as 5 M
Anderson, D. et al. (2010). Albumin-based microbubbles bind upregulated scavenger receptors following vascular injury. J. Biol.
Chem. 285:40645-40653. (STA-318)
1.8
1.8
1.6
1.6
1.4
1.4
1.2
OD 340nm
OD 340nm
Advanced oxidation protein products are toxins created during oxidative stress in patients with diabetes
mellitus, atherosclerosis, renal complications, and
HIV. Our OxiSelect AOPP Assay Kit provides a
quick, easy method for assessing AOPP levels.
1
0.8
0.6
0.4
1.2
1
0.8
0.6
0.4
0.2
0.2
0
0
20
40
60
80
100
120
Free HSA
Chloramine-T (M)
Chloramine Standard Curve Generated with the OxiSelect

AOPP Assay Kit.
Product Name
OxiSelect AOPP Assay Kit
AOPP-Human Serum Albumin
AOPP-HSA
Untreated Human Serum Albumin and AOPP-HSA Positive

Control Tested with the OxiSelect AOPP Assay Kit.
Detection
Size
Catalog Number
Colorimetric
200 Assays
STA-318
N/A
50 L
STA-319
OxiSelect BPDE Protein Adduct ELISA Kit

Polycyclic aromatic hydrocarbons (PAH) are potent
carcinogenic pollutants commonly associated with oil,
cigarette smoke, and automotive exhaust. They may
also be found in some cooked foods. One PAH,
benzo(a)pyrene, was the first chemical carcinogen to
be discovered. Through a series of enzymatic reactions, benzo(a)pyrene is converted to benzo(a)pyrene
7,8 diol-9,10 epoxide (BPDE) which attacks both proteins and DNA.
Our OxiSelect BPDE Protein Adduct ELISA Kit
provides a convenient method to measure the
modification of proteins by BPDE.
Sensitive: Detect concentrations as low as 60
ng/mL
Convenient: Quantify on a standard microplate
reader
Versatile: Suitable for use with cell lysates, tissue homogenates, plasma or serum
Product Name
OxiSelect BPDE Protein Adduct ELISA Kit
80
Phone 1-858-271-6500
BPDE-BSA Standard Curve Generated Using the OxiSelect

BPDE Protein Adduct ELISA Kit.
For information on our BPDE DNA Adduct

ELISA Kit, please see page 90.
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-301
Fax 1-858-271-6514
Protein Oxidation
OxiSelect Kidney Injury Molecule-1 (KIM-1) Assays

KIM-1 is an excellent marker of kidney tubular toxicity. It also has been shown to associate with spatial
expression of nitrotyrosine and inducible nitric oxide
synthase. Our OxiSelect KIM-1 Assays are useful
for detecting this marker in urine. For quick results
choose one of our lateral flow detection (dipstick)
kits. Our KIM-1 ELISA kits provide unsurpassed results when sensitivity is critical.
Sensitive: Detect concentrations as low 300 pg/mL

using the ELISA Kit format
Fast: Obtain results in 15 minutes using the Lateral
Flow Detection Kit format
Color Comparison Chart for the OxiSelect Human KIM-1

Lateral Flow Detection Kit.
Standard Curve Generated with the OxiSelect Human

KIM-1 ELISA Kit. Concentrations are expressed in ng/mL.
Product Name
Detection
Size
Catalog Number
OxiSelect Human KIM-1 ELISA Kit
Colorimetric
96 Assays
STA-374
10 Assays
STA-370
OxiSelect Human KIM-1 Lateral Flow Detection Kit
Lateral Flow
50 Assays
STA-371
96 Assays
STA-376
10 Assays
STA-372
50 Assays
STA-373
Size
Catalog Number
Copper (Cu++) Oxidized Human Low Density Lipoprotein (LDL)
100 g
STA-214
Malondialdehyde (MDA) Modified Human Albumin
100 g
STA-210
Malondialdehyde (MDA) Modified Human Apolipoprotein B-100
100 g
STA-211
Malondialdehyde (MDA) Modified Human Low Density Lipoprotein (LDL)
100 g
STA-212
Nitrated Human Low Density Lipoprotein (LDL)
100 g
STA-213
OxiSelect Rat Kim-1 ELISA Kit
Colorimetric
OxiSelect Rat Kim-1 Lateral Flow Detection Kit
Lateral Flow
Oxidized / Nitrated Proteins

All proteins are provided at a concentration of 1.0 mg/mL.
Product Name
www.cellbiolabs.com
81
Lipid Peroxidation
Assays and Reagents for Lipid Peroxidation

Lipid peroxidation is a well-defined mechanism of cellular damage in both animals and
plants that occurs during aging and in some disease states. Our OxiSelect Lipid Peroxidation Assays allow you to quickly and easily quantify the most common markers and byproducts of lipid peroxidation.
OxiSelect HNE ELISA Kits and Antibodies

4-hydroxynonenal (4-HNE) is a well-known byproduct of lipid peroxidation and is widely accepted
as a stable marker for oxidative stress. HNE protein
adducts are typically stable when frozen for up to 6
months or more.
Our OxiSelect HNE Adduct ELISA Kit provides a
simple, user-friendly way to assess HNE adduct formation on lysine, histidine and/or cystein. The OxiSelect HNE-His Adduct ELISA Kit specifically recognizes HNE modification of histidine residues.
Our polyclonal antibodies against HNE recognize
HNE adducts to lysine, histidine, and/or cysteine.
Sensitive: ELISA kit detects protein adducts as

low as 150 ng/mL
Versatile: Suitable for use with serum, plasma,
cell lysates or tissue homogenates

1. Radu, R.A. et al. (2011). Complement system dysregulation
and inflammation in the retinal pigment epithelium of a mouse
model for Stargardt macular degeneration. J. Biol. Chem.
286:18593-18601. (STA-334)
2. Sinha-Hikim, I. et al. (2010). Effects of a novel cysteine-based
glutathione precursor on oxidative stress in vascular smooth
muscle cells. Am. J. Physiol. Cell Physiol. 299:C638-C624.
(STA-334)
3. Mukhopadhyay, P. et al. (2010). CB1 cannabinoid receptors
promote oxidative stress and cell death in murine models of
doxorubicin-induced cardiomyopathy in human cardiomyocytes. Cardiovasc. Res. 85:773-784. (STA-334)
4. Maki, R.A. et al. (2009). Aberrant expression of myeloperoxidase in astrocytes promotes phospholipid oxidation and memory deficits in a mouse model of Alzheimers disease. J. Biol.
Chem. 284:3158-3169. (STA-334)
5. Singh, R. et al. (2009). Chronic oxidative stress sensitizes
hepatocytes to death from 4-hydroxynonenal by JNK/c-Jun
overactivation. Am. J. Physiol. Gastrointest. Liver Physiol.
297:G907-G917. (STA-334)
6. Resende, R. et al. (2008). Brain oxidative stress in a tripletransgenic mouse model of Alzheimers disease. Free Radic.
Biol. Med. 44(12):2051-2057. (STA-334)
HNE Adduct ELISA Standard Curve.

Product Name
Detection
OxiSelect HNE Adduct ELISA Kit
Catalog Number
96 Assays
STA-338
5 x 96 Assays
STA-338-5
96 Assays
STA-334
5 x 96 Assays
STA-334-5
Colorimetric
OxiSelect HNE-His Adduct ELISA Kit
Colorimetric
Goat Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-034
Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-035
N/A
100 g
STA-335
HNE-BSA Control
82
Size
Phone 1-858-271-6500
Fax 1-858-271-6514
Lipid Peroxidation
OxiSelect 8-iso-Prostaglandin F2 ELISA Kit (8-isoprostane)

8-iso-Prostaglandin F2 is produced in membrane
phospholipids and has been implicated in atherogenesis, rheumatoid arthritis and carcinogenesis.
The OxiSelect 8-iso-Prostaglandin F2 ELISA Kit
provides rapid and sensitive detection of 8-iso-PGF2
as low as 50 pg/mL.
Product Name

Ungvari, Z. et al. (2011). Age-associated vascular oxidative stress,
Nrf2 dysfunction, and NFkB activation in the nonhuman primate
Macaca mulatta. J. Gerontol A Biol. Sci. Med. Sci. 10.1093/gerona/
glr092. (STA-337)
Detection
Size
Catalog Number
96 Assays
STA-337
5 x 96 Assays
STA-337-5
Colorimetric
OxiSelect 8-iso-Prostaglandin F2 ELISA Kit
OxiSelect TBARS Assay Kit

The TBARS assay is a well-established method for
screening and monitoring lipid peroxidation via the
by-product malondialdehyde (MDA). MDA forms a
1:2 adduct with thiobarbituric acid.
Our OxiSelect TBARS Assay Kit provides a more
user-friendly protocol for quantitation of the MDATBA adduct compared to other commercial assays.
Colorimetric Assay
0.45
0.4
OD 540nm
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
0
10
20
30
40
MDA (M)
MDA-TBA Standard Curve Using a Standard Plate Reader.
Product Name
OxiSelect TBARS Assay Kit (MDA Quantitation)
Fast: Obtain results in 30 minutes

Sensitive: Smaller reaction volumes require less
sample; detect as little as 2 M
Convenient: No marbles or glass tubes required
as with other TBARS assays
1. Kasaikina, M.V. et al. (2011). Roles of the 15-kDa Selenoprotein (Sep15) in redox homeostasis and cataract development
revealed by the analysis of Sep15 knockout mice. J. Biol.
Chem. 286:33203-33212. (STA-330)
2. Fedeles, B.I. et al. (2011). Chemical genetics analysis of an
aniline mustard anticancer reveals complex I of the electron
transport chain as a target. J. Biol. Chem. 286:33910-33920.
(STA-330)
3. Kwon, H. et al. (2010). Vitamin D3 upregulated protein 1 suppresses TNF-alpha induced NFkB activation in hepatocarcinogenesis. J. Immunol. 185:3980-3989. (STA-330)
4. Wojciechowski, P. et al. (2010). Resveratrol arrests and regresses the development of pressure overload- but not volume
overload-induced cardiac hypertrophy in rats. J. Nutr. 140:962968. (STA-330)
5. Song, Y.R. et al. (2010). Activation of hypoxia-inducible factor
attenuates renal injury in rat remnant kidney. Nephrol. Dial.
Transplant. 25:77-85. (STA-330)
6. Fomenko, D.E. et al. (2009). Methionine-R-sulfoxide reductase
1 (MsrB1) knockout mice: Roles of MsrB1 in redox regulation
and identification of a novel selenoprotein form. J. Biol. Chem.
284:5986-5993. (STA-330)
7. Fujita, K. et al. (2008). Effectiveness of antiplatelet drugs
against experimental non-alcoholic fatty liver disease. Gut
57:1583-1591. (STA-330)
Detection
Size
Colorimetric or
Fluorometric
200 Assays
STA-330
5 x 200 Assays
STA-330-5
www.cellbiolabs.com
Catalog Number
83
Lipid Peroxidation
OxiSelect MDA (Malondialdehyde) Assays and Antibodies
Our OxiSelect MDA Immunoblot Kit provides a

more direct method to quantify MDA formation compared to the traditional TBARS Assay. A positive immunoblot control is provided in the kit. The OxiSelect MDA ELISA Kit offers a higher throughput format to accommodate a larger number of samples.
Note: MDA is most reliably detected on fresh samples, or samples that have been frozen for a maximum of 60 days. For samples stored for longer periods, consider testing other markers of lipid peroxidation such as 4-HNE or 8-isoprostane.
Immunoblot of
MDA-BSA Control Using the
OxiSelect MDA
Immunoblot Kit.
Immunoblot control was electroblotted onto a nitrocellulose membrane, followed by
detection with the
provided antiMDA antibody.
Sensitive: ELISA kit detects MDA protein adducts as low as 2 pmol/mg

Versatile: Suitable for use with serum, plasma,
cell lysates or tissue homogenates
1. Lazrak, A. et al. (2011). Regulation of alveolar epithelial Na+
channels by ERK1/2 in chlorine breathing mice. Am. J. Respir.
Cell Mol. Biol. 10.1165/rcmb.2011-0309OC. (STA-331)
2. Zarogiannis, S.G. et al. (2011). Ascorbate and deferoxamine
administration post chlorine exposure decrease mortality and
lung injury in mice. Am. J. Respir. Cell Mol. Biol. 45:386-392.
(STA-331)
3. Hall, J.A. et al. (2010). Absence of thyroid hormone activation
during development underlies a permanent defect in adaptive
thermogenesis. Endrocrinology 151:4573-4582. (STA-331)
4. Barabutis, N. et al. (2008). Antioxidant activity of growth hormone-releasing hormone antagonists in LNCaP human prostate cancer cell line. PNAS 105:20470-20475. (STA-331)
5. Schinzari, F. et al. (2010). Generalized impairment of vasodilator reactivity during hyperinsulinemia in patients with obesityrelated metabolic syndrome. Am. J. Physiol. Endocrinol. Metab.
299:E947-E952. (STA-332)
6. Labbe, A. et al. (2010). Resveratrol improves insulin resistance
hyperglycemia and hepatosteatosis but not hypertriglyercidemia, inflammation, and life span in a mouse model for Werner
syndrome. J. Gerontol. A. Biol. Sci. Med. Sci. 66:741-750.
(STA-332)
2
1.8
1.6
1.4
OD 450 nm
As a common by-product of lipid peroxidation,

malondialdehyde (MDA) is a well-accepted marker of
oxidative stress. Modification of proteins by MDA can
cause structural and functional changes in oxidized
proteins.
1.2
1
0.8
0.6
0.4
0.2
0
0
20
40
60
80
100
120
140
MDA Adduct (pmol/mg)
MDA-BSA Standard Curve Generated with the OxiSelect

MDA Adduct ELISA Kit.
Product Name
Detection
Size
Catalog Number
OxiSelect MDA Immunoblot Kit
Immunoblot
10 Blots
STA-331
96 Assays
STA-332
OxiSelect MDA Adduct ELISA Kit
Colorimetric
5 x 96 Assays
STA-332-5
Goat Anti-Malondialdehyde (MDA) Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-031
Rabbit Anti-Malondialdehyde (MDA) Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-032
N/A
100 g
STA-333
MDA-BSA Control
84
Phone 1-858-271-6500
Fax 1-858-271-6514
Oxidized LDL
OxiSelect Human Oxidized LDL ELISA

Low density lipoprotein (LDL) contains a hydrophobic
core of various lipids surrounded by one molecule of
Apolipoprotein B-100 (ApoB-100), which promotes
solubility of the LDL in blood. LDL is often described
as bad cholesterol, but it is even more dangerous in
the human body when it becomes oxidized. Oxidized
LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner lining of arteries.
The majority of oxidized residues in LDL are
malondialdehyde (MDA) adducts.
Sensitive: Detect as little as 50 ng/mL

Quantitative: Compare unknown samples with
provided copper oxidized LDL standard
Our OxiSelect Human Oxidized LDL ELISA Kit is

designed for the detection and quantitation of MDAmodified LDL in human plasma or serum.

Oxidized LDL ELISA Kit.
OxiSelect Human Oxidized LDL ELISA Assay Principle.

Product Name
OxiSelect Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation)
Quantitation of OxLDL in Serum and Plasma Samples. Serum

and plasma samples were treated with LDL Precipitation Solution.
Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further dilution 1:160 in Assay Diluent according to the Assay
Protocol.
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-369
www.cellbiolabs.com
85
DNA / RNA Damage & Repair
Assays for DNA & RNA Damage and Repair

DNA is arguably the most biologically significant target of oxidative and cellular stress.
Continuous DNA damage has been implicated in age-related development of various cancers. More recently, RNA damage has been described in conjunction with various neurological diseases including Alzheimers and Parkinsons diseases. We offer a wide range of
assays to measure the most common types of DNA and RNA damage in cells.
OxiSelect Oxidative DNA / RNA Damage ELISA Kits

(8-OHdG or 8-OHG Quantitation)
Among numerous types of oxidative DNA damage, 8
-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous
marker of oxidative stress. 8-OHdG, one of the byproducts of DNA oxidative damage, is physiologically formed and enhanced by chemical carcinogens.
Our OxiSelect Oxidative DNA Damage ELISA Kit
provides a powerful method for rapid quantitation of
8-OHdG in DNA samples. The OxiSelect Oxidative RNA Damage ELISA Kit uses the same assay
principle, but is useful for measuring 8-OHG formation, the primary marker for RNA oxidation.
Highly Sensitive: Detect as little as 100 pg/

mL of 8-OHdG or 150 pg/mL of 8-OHG
Versatile: Suitable for use with urine, serum,
cerebrospinal fluid, and cell or tissue samples
1. Barsony, J. et al. (2011). Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone
loss. J. Biol. Chem. 286:10864-10875. (STA-320)
2. Li, M. et al. (2010). The ATM-p53 pathway suppresses aneuploidy-induced tumorigenesis. PNAS 107:14188-14193. (STA320)
3. Hasegawa, T. et al. (2009). Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. Am.J.
Physiol. Hear Circ. Physiol. 296:H1007-H1016. (STA-320)
4. Pialoux, V. et al. (2009). Effects of exposure to intermittent
hypoxia on oxidative stress and acute hypoxic ventilatory
response in humans. Am. J. Respir. Crit. Care Med. 180:1002
-1009. (STA-320)
2.5
2.5
2
OD 450 nm
1.5
1
1.5
1
0.5
0
on
tr
ol
di
lu
t io
n
di
lu
t io
n
di
lu
t io
n
di
lu
t io
n
di
lu
t io
n
di
lu
t io
n
0.5
Standard Curve Generated with the OxiSelect Oxidative

DNA Damage ELISA Kit.
Product Name
OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation)
Phone 1-858-271-6500
0
1/
64
1/
32
8-OHdG Levels in a Human Urine Sample.

Detection
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation)
86
1/
16
8-OHdG (ng/mL)
1/
80
100
1/
40
10
tiv
0.1
eg
a
0.01
1/
20
OD 450 nm
Size
Catalog Number
96 Assays
STA-320
5 x 96 Assays
STA-320-5
96 Assays
STA-325
5 x 96 Assays
STA-325-5
Colorimetric
Colorimetric
Fax 1-858-271-6514
OxiSelect Comet Assays (Single Cell Gel Electrophoresis)

DNA damage can result from a variety of intracellular and extracellular stimuli, and can manifest in a
variety of mutations to the DNA including base modifications, missing bases and single-stranded or double-stranded breaks.
Versatile: High-level screening tool for DNA

damage from a wide variety of sources
User Friendly: Simple protocol with easy visualization by epifluorescence microscopy
Traditionally the comet assay, or single cell gel electrophoresis (SCGE), has been used as a wellpublished, high-level screening tool to measure DNA
damage in single cells.
Our OxiSelect Comet Assay Kits provide a quick,
easy method to screen for DNA damage at a macro
level. Our OxiSelect Comet Assay Slides have
been specially treated for adhesion of low-melting
agarose used in the assay. Damaged DNA moves
farther in electrophoresis than intact DNA, causing a
tail to form upon visualization under a fluorescence
microscope.
Tyagi, A. et al. (2011). Resveratrol selectively induces DNA
damage, independent of Smad4 expression, in its efficacy
against human head and neck squamous cell carcinoma. Clin.
Cancer Res. 17:5402-5411. (STA-355)
Etoposide Treatment of Jurkat Cells. Jurkat cells were either

untreated (left) or treated with etoposide (right) prior to performing the OxiSelect Comet Assay. Assay was run under alkaline
conditions at 33 V / 300 mA for 15 minutes.
Product Name
OxiSelect 3-Well Comet Assay Kit
OxiSelect 3-Well Comet Assay Slides
OxiSelect 96-Well Comet Assay Kit
OxiSelect 96-Well Comet Assay Slides

OxiSelect Comet Assay Control Cells
(includes positive and negative controls)
Assay Principle for the OxiSelect Comet Assay Kit.
Detection
Light Microscopy
Light Microscopy
Size
Catalog Number
15 Wells
STA-350
75 Wells
STA-351
5 x 75 Wells
STA-351-5
5 Slides
STA-352
25 Slides
STA-353
125 Slides
STA-353-5
96 Wells
STA-355
5 x 96 Wells
STA-355-5
1 Slide
STA-356
5 Slides
STA-356-5
1 Set
STA-354
Light Microscopy
Light Microscopy
N/A
www.cellbiolabs.com
87
OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites)
Our OxiSelect Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for
measuring AP sites in DNA. The assay uses an aldehyde reactive probe (ARP) which specifically reacts with an aldehyde group on the open ring of the
AP site, followed by labeling with Biotin and subsequent detection by Streptavidin-enzyme conjugate.
1.8
1.6
1.4
OD 450 nm
Oxidative DNA Damage can manifest in the formation of apurinic or apyrimidinic (AP) sites, also
known as loss of bases. Spontaneous base loss, if
unrepaired, can inhibit transcription and may be
mutagenic.
1.2
1
0.8
0.6
0.4
0.2
0
0
Highly Sensitive: Detect as few as 4-40 AP

sites in 105 bp of DNA
Versatile: Suitable for use with genomic DNA
from cells or tissues
Quantitative: Kit includes both oxidized and
reduced DNA standards for absolute quantitation
Product Name
OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites)
10
20
30
40
50
AP Sites per 100,000bp

Standard Curve Generated with the OxiSelect Oxidative
DNA Damage Quantitation Kit (STA-324).
Detection
Size
Catalog Number
Colorimetric
50 Assays
STA-324
OxiSelect DNA Double-Strand Break Assay

Double-strand breaks (DSB) are among the most
dangerous types of DNA damage within cells. An
early cellular response is phosphorylation of the histone variant H2AX at the site of the DSB. This triggers a cascade of events and appears to play a role
in recruitment of repair factors to the damaged sites.
Our OxiSelect DNA Double-Strand Break Staining
Kit provides an easy-to-use method for detecting
DNA breaks. The kit utilizes simple immunofluorescence staining of the phosphorylated histone H2AX.
Fast: See staining results in about 3 hours

Positive Control: DNA Double-strand break
inducer included in kit
Product Name
OxiSelect DNA Double-Strand Break Staining Kit
88
Phone 1-858-271-6500
DNA Double-Strand Break Formation in A549 Cells. A549

cells were seeded at 50,000 cells/well overnight. Immunofluorescence staining was then performed according to the assay protocol. (A) Untreated cells. (B) Cells treated with 100 M etoposide for one hour.
Detection
Size
Catalog Number
Immunofluorescence
100 Assays
STA-321
Fax 1-858-271-6514
OxiSelect Cellular UV-Induced DNA Damage Assays

Absorption of ultraviolet radiation can damage DNA
by the formation of pyrimidine dimers. The two main
forms of pyrimidine dimers are cyclobutane
pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP).
Our OxiSelect Cellular UV-Induced DNA Damage
Assays conveniently measure the formation of either
CPD or 6-4PP in intact cells. Kits for each marker are
available in two formats: ELISA and Immunostaining.
UV-Induced DNA Damage in HeLa Cells Treated with Ultraviolet Light for 30 Minutes and Visualized with the OxiSelect
Cellular UV-Induced DNA Damage Staining Kit (CPD).
1.6
1.4
OD 450nm
1.2
1.0
0.8
0.6
0.4
0.2
0.0
Secondary
Antibody Alone
Formation of Pyrimidine Dimers CPD and 6-4PP from

Exposure to Ultraviolet Radiation.
Product Name
UV Treated
Untreated
UV-Induced DNA Damage in HeLa Cells as Detected with the

OxiSelect Cellular UV-Induced DNA Damage ELISA (CPD).
Detection
Size
Catalog Number
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD)
Colorimetric
96 Assays
STA-326
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (6-4PP)
Colorimetric
96 Assays
STA-328
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)
Fluorescence
Microscopy
96 Assays
STA-327
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP)
Fluorescence
Microscopy
96 Assays
STA-329
OxiSelect UV-Induced DNA Damage ELISA Kits

These kits are similar to the Cellular UV-Induced
Damage Assays above, except they are designed for
use with DNA extracted from cells or tissues. The
Combo Kit allows detection of both CPD and 6-4PP
on a single plate, but in different wells.
Product Name

Burgess, H.M. et al. (2011). Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to
UV irradiation reveals mRNA-dependent export of metazoan
PABPs. J. Cell Sci. 124:3344-3355. (STA-322)
Detection
Size
Catalog Number
OxiSelect UV-Induced DNA Damage ELISA Combo Kit (CPD/6-4PP)
Colorimetric
96 Assays
STA-322-C
96 Assays
STA-322
OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation)
Colorimetric
5 x 96 Assays
STA-322-5
96 Assays
STA-323
OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation)
Colorimetric
www.cellbiolabs.com
89
Checkpoint Kinase Activity Assays

Checkpoint kinases, specifically CHK1 and CHK2,
are activated in response to DNA damage to subsequently phosphorylate Cdc25C prior to mitosis, which
prompts cell cycle arrest. Mutation of these checkpoint kinases can ultimately lead to decreased DNA
repair.
CHK1 Activity Using the

Checkpoint Kinase Activity Immunoblot Kit. Lane 1:
Negative Control; Lane 2:
10 ng of active CHK1.
Our Checkpoint Kinase Activity Assays allow you to

conveniently measure the activity of CHK1 and
CHK2. The assays use recombinant Cdc25C as a
checkpoint kinase substrate. Phosphorylated
Cdc25C (Ser216) is detected using a phosphospecific antibody. Checkpoint Kinase Activitiy Assays are available in two formats: a Western blot
assay and a 96-well plate-based activity assay.
Product Name
Detection
Size
Catalog Number
Checkpoint Kinase Activity Immunoblot Kit
Immunoblot
20 Assays
STA-413
96 Assays
STA-414
96-Well Checkpoint Kinase Activity Assay Kit
Colorimetric
5 x 96 Assays
STA-414-5
OxiSelect BPDE DNA Adduct ELISA Kit

Polycyclic aromatic hydrocarbons (PAH) are potent
carcinogenic pollutants commonly associated with oil,
cigarette smoke, and automotive exhaust. They may
also be found in some cooked foods. One PAH,
benzo(a)pyrene, was the first chemical carcinogen to
be discovered. Through a series of enzymatic reactions, benzo(a)pyrene is converted to benzo(a)pyrene
7,8 diol-9,10 epoxide (BPDE) which attacks both proteins and DNA.
Our OxiSelect BPDE DNA Adduct ELISA Kit provides a convenient method to measure BPDE adducts in DNA extracted from cells or tissues.
Sensitive: Detect concentrations as low as 30
ng/mL
reader
Product Name
OxiSelect BPDE DNA Adduct ELISA Kit
90
Phone 1-858-271-6500
BPDE-DNA Standard Curve Generated Using the OxiSelect

BPDE DNA Adduct ELISA Kit.
For information on our BPDE Protein

Adduct ELISA Kit, please see page 80.
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-357
Fax 1-858-271-6514
Global Methylation and Hydroxymethylation ELISA Kits

DNA methylation is an epigenetic change shown to
be associated with nearly every biological process. In
mammalian cells, DNA methylation is found predominantly at CpG dinucleotides; however, in certain
cases such as embryonic stem cells it may also be
found in non-CpG contexts. Due to the important role
of DNA methylation in maintaining genomic stability,
deregulation of DNA methylation is associated with
various diseases including cancer.
Sensitive: Detect as little as 15 nM of 5MedCyd

or 5 ng of hydroxymethylated DNA
Versatile: Suitable for use with any isolated
DNA; 5MedCyd may also be used with urine
samples
reader
5-hydroxymethylcytosine (5-hmC) results from enzymatic conversion of 5-methylcytosine (5-mC); though

its epigenetic functions are still being elucidated, 5hmC has been found to be more abundant in brain
and stem cells. Bisulfite sequencing is unable to distinguish 5-hmC from 5-mC; other methods such as
HPLC or LC/MS can distinguish the two, but such
methods are labor intensive and not always practical.
Our Global DNA Methylation and Hydroxymethylation Assays provide a convenient, accurate way to
quantify 5-methyl-2-deoxycytidine (5MedCyd) and
5-hydroxymethylcytosine respectively. Unknown
samples are compared with a standard provided
with each kit.
Methylated DNA Standard Curve Generated with the Global

DNA Methylation ELISA Kit.
Product Name
Hydroxymethylated DNA Standard Curve Generated with the

Global DNA Hydroxymethylation ELISA Kit.
5MedCyd Levels in Human Urine Sample as Measured with

the Global DNA Methylation ELISA Kit.
Detection
Global DNA Methylation ELISA Kit

(5-methyl-2-deoxycytidine Quantitation)
Colorimetric
Global DNA Hydroxymethylation ELISA Kit

(5-Hydroxymethylcytosine Quantitation)
Colorimetric
www.cellbiolabs.com
Size
Catalog Number
96 Assays
STA-380
5 x 96 Assays
STA-380-5
96 Assays
STA-381
91
ROS Assays
Reactive Oxygen Species Assays

Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide are continually
produced during metabolic processes. Excess ROS can lead to cellular injury in the form of
damaged DNA, lipids and proteins. We offer assays for quantitation of various reactive oxygen species, in both in vitro and intracellular formats.
OxiSelect Intracellular ROS Assay Kit

The OxiSelect Intracellular ROS Assay Kit measures
the activity of hydroxyl, peroxyl, and other reactive oxygen species. The assay uses the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is
deacetylated into the non-fluorescent DCFH. In the
presence of ROS, the DCFH is oxidized into highly
fluorescent DCF. Fluorescence is quantified on a
fluorometric plate reader.
Sensitive: Detect concentrations as little as 10 pM
Fast: Entire protocol takes about one hour
2000
1800
1600
1400
RFU
1200
1000
800
600
400
200
0
0 M
100 M
1000 M
H2O2 Concentration
Measurement of ROS in HeLa Cells. 50,000 HeLa cells in a 96well plate were pretreated with 1mM DCFH-DA for 60 minutes at
37C. Cells were then treated with hydrogen peroxide for 20 min.
1. Batova, A. et al. (2010). The synthetic caged garcinia xanthone
cluvenone induces cell stress and apoptosis and has immune
modularity activity. Mol. Cancer Ther. 9:2869-2878. (STA-342)
2. Zhang, Y. et al. (2010). The mitochondrial pathway of anesthetic
isofluorane-induced apoptosis. J. Biol. Chem. 285:4025-4037.
(STA-342)
3. Tanaka, N. et al. (2010). Cis-dichlorodiammineplatinum upregulates angiotensis II type 1 receptors through reactive oxygen
species generation and enhances VEGF production in bladder
cancer. Mol. Cancer Ther. 9:2982-2992. (STA-342)
Product Name
OxiSelect Intracellular ROS Assay Kit
92
Phone 1-858-271-6500
Assay Principle for the OxiSelect ROS Assay Kit.

Detection
Size
Catalog Number
Fluorometric
96 Assays
STA-342
Fax 1-858-271-6514
ROS Assays
OxiSelect In Vitro ROS/RNS Assay Kit

The OxiSelect In Vitro ROS/RNS Assay Kit measures the activity of hydrogen peroxide, nitric oxide,
peroxynitrite, peroxyl radicals, and other reactive oxygen and reactive nitrogen species. The assay principle is similar to our Intracellular ROS Assay (previous
page), except that the chemistry is modified to allow
detection of ROS outside the cell in various sample
types. Fluorescence is quantified on a fluorometric
plate reader.
Sensitive: Detect concentrations as little as 10 pM
for DCF or 40 nM for hydrogen peroxide
Fast: Entire protocol takes about one hour
Versatile: Suitable for a wide variety of sample
types including urine, serum, plasma, cell lysates,
tissue homogenates and cell culture supernatants
Detection of Peroxyl Radicals Using the OxiSelect In Vitro

ROS/RNS Assay Kit.
Product Name
OxiSelect In Vitro ROS/RNS Assay Kit
Detection
Size
Catalog Number
Fluorometric
96 Assays
STA-347
OxiSelect Hydrogen Peroxide Assays

Our OxiSelect Peroxide Assay Kits provide a simple method for quantitation of hydrogen peroxide and/
or peroxidases.
Our colorimetric assay measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions in the presence of
peroxides. The ferric ions form a complex with a provided dye which may be read on a standard microplate reader. The assay may be run with either
aqueous phase or lipid phase samples.
The fluorometric assay uses a probe which is converted from a non-fluorescent to a fluorescent state in
the presence of peroxides and catalyzed by peroxidases.
Sensitive: Detect as little as 50 nM (fluorometric
format) or 1 M (colorimetric format)
Fast: Easy 30 minute incubation
Versatile: Suitable for plasma, cell fractions, tissue
lysates, solid and aqueous nutrition samples
Product Name
Standard Curve Generated with the OxiSelect Peroxide

Detection Assay (Fluorometric).
Detection
Size
Catalog Number
OxiSelect Hydrogen Peroxide Assay Kit
Colorimetric
500 Assays
STA-343
OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit
Fluorometric
500 Assays
STA-344
www.cellbiolabs.com
93
Antioxidant Assays
Antioxidant Assays
ROS generation is normally counterbalanced by the action of antioxidant enzymes and
other redox molecules. We offer two types of assays for antioxidant quantitation:
Assays to quantify the presence of antioxidant molecules

Assays to determine the antioxidant capacity of biomolecules
OxiSelect Superoxide Dismutase Activity Assay

Superoxide dismutase (SOD), which catalyzes the
dismutation of the superoxide anion into hydrogen
peroxide and molecular oxygen, is one of the most
important antioxidant enzymes.
0.8
OD 490nm
The OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system
to generate superoxide anions and a chromagen to
produce a water-soluble dye upon reduction by the
superoxide anions. SOD activity is determined as
the inhibition or reduction of chromagen.
1
0.9
0.7
0.6
0.5
0.4
0.3
0.2
0.1
Sensitive: Detect as little as 0.6 units/mL

Fast: Obtain results in about 2 hours
Quantitative: SOD standard included as positive
control
Versatile: Suitable for use with urine, serum, cells
or tissue samples
Product Name
OxiSelect Superoxide Dismutase Activity Assay
0
0.001
0.01
0.1
10
100
SOD (Units)
Standard Curve Using the OxiSelect Superoxide Dismutase
Activity Assay.
Detection
Size
Catalog Number
Colorimetric
100 Assays
STA-340
OxiSelect Catalase Activity Assay Kits

Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress.
Our OxiSelect Catalase Activity Assay Kits provide
a quick, user-friendly protocol to monitor catalase
activity from a variety of sample types. Kits are available with either colorimetric or fluorometric detection.
Product Name
Sensitive: Detect as little as 1.25 units/mL

(colorimetric) or 50 mU/mL (fluorometric)
Fast: Obtain results in less than 30 minutes
Versatile: Suitable for use with whole blood,
plasma, serum, cell lysates or tissue homogenates
High Throughput: 96-well format
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-341
Fluorometric
96 Assays
STA-339
OxiSelect Catalase Activity Assay Kit
94
Phone 1-858-271-6500
Fax 1-858-271-6514
Antioxidant Assays
OxiSelect Total Glutathione (GSSG/GSH) Assay Kit

Glutathione is an intracellular tripeptide thiol that
helps protect cells from free radical damage by acting as an antioxidant. Glutathione exists within cells
in both reduced (GSH) and oxidized (GSSG) forms;
it is involved in the breakdown of peroxides and also
helps maintain exogenous antioxidants such as vitamins C and E.
The OxiSelect Total Glutathione Assay Kit is a
quantitative assay for measuring total GSH/GSSG
content in a variety of sample types.
Sensitive: Detect as little as 8 nM total glutathione
Fast: Obtain results in less than 30 minutes
Versatile: Suitable for use with saliva, urine,
serum, plasma, cells or tissue extracts
Product Name
OxiSelect Total Glutathione (GSSG/GSH) Assay Kit
Total Glutathione Content in NIH3T3 Cell Lysate.
Detection
Size
Catalog Number
Colorimetric
100 Assays
STA-312
OxiSelect Total Antioxidant Capacity (TAC) Assay Kit

The OxiSelect Total Antioxidant Capacity (TAC)
Assay Kit measures the total antioxicant capacity of
biomolecules from a variety of sample types via a
Single Electron Transfer (SET) mechanism. In the
presence of antioxidants, copper(II) is reduced to
copper(I). In turn, the copper(I) ions react with a
chromogen to produce a color with a maximum absorbance at 490 nm. The assay works with a variety
of antioxidants and is suitable for testing plasma,
serum, urine, cell lysates, tissue homogenates and
food extracts.
Measurement of Various Antioxidant Molecules using the

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit.
Product Name
OxiSelect Total Antioxidant Capacity (TAC) Assay Kit
TAC Assay Principle.
Detection
Size
Catalog Number
Colorimetric
200 Assays
STA-360
www.cellbiolabs.com
95
Antioxidant Assays
OxiSelect ORAC Activity Assay Kit

The assay known as ORAC (Oxygen Radical Antioxidant Capacity) is a powerful tool to measure the
antioxidant capacity of biomolecules. The assay
measures antioxidant capacity against peroxyl radicals. Our OxiSelect ORAC Activity Assay Kit
measures antioxidant capacity quickly and easily in
a variety of sample types.
Sensitive: Detect as little as 2.5 M
Versatile: Suitable for use with plasma, cell
fractions, tissue lysates, solid and aqueous
nutrition samples

1. Bailey-Downs, L.C. et al. (2011). Liver-specific knockdown of
IGF-1 decreases vascular oxidative stress resistance by impairing the Nrf2-dependent antioxidant response: a novel
model of vascular aging. J. Gerontol. A. Biol. Sci. Med. Sci.
10.1093/gerona/glr164.
longest living non-colonial animal. J. Gerontol. A. Biol. Sci.
Med. Sci. 10.1093/gerona/glr044.
3. Ungvari, Z. et al. (2011). Free radical production, antioxidant
capacity, and oxidative stress response signatures in fibroblasts from Lewis dwarf rats: effects of life span-extending
peripubertal GH treatment. J. Gerontol. A. Biol. Sci. Med. Sci.
66:501-505.
Assay Principle for the OxiSelect Oxygen Radical Antioxidant Capacity (ORAC) Assay.
Product Name
Detection
OxiSelect ORAC Activity Assay Kit
Size
Catalog Number
192 Assays
STA-345
5 x 192 Assays
STA-345-5
Fluorometric
OxiSelect HORAC Activity Assay Kit

The HORAC (Hydroxyl Radical Antioxidant Capacity)
assay, like the ORAC assay above, is a powerful tool
to measure the antioxidant capacity of biomolecules.
This assay specifically measures antioxidant capacity
against hydroxyl radicals. Our OxiSelect HORAC
Activity Assay Kit measures antioxidant capacity
quickly and easily in a variety of sample types.
Fast: Obtain results in less than 2 hours on a
fluorometric plate reader
Versatile: Suitable for plasma, cell fractions, tissue
lysates, solid and aqueous nutrition samples
Product Name
OxiSelect HORAC Activity Assay Kit
96
Phone 1-858-271-6500

1. Bailey-Downs, L.C. et al. (2011). Liver-specific knockdown of
IGF-1 decreases vascular oxidative stress resistance by impairing the Nrf2-dependent antioxidant response: a novel
model of vascular aging. J. Gerontol. A. Biol. Sci. Med. Sci.
10.1093/gerona/glr164.
longest living non-colonial animal. J. Gerontol. A. Biol. Sci.
Med. Sci. 10.1093/gerona/glr044.
3. Ungvari, Z. et al. (2011). Free radical production, antioxidant
capacity, and oxidative stress response signatures in fibroblasts from Lewis dwarf rats: effects of life span-extending
peripubertal GH treatment. J. Gerontol. A. Biol. Sci. Med. Sci.
66:501-505.
Detection
Size
Catalog Number
192 Assays
STA-346
5 x 192 Assays
STA-346-5
Fluorometric
Fax 1-858-271-6514
CELL SIGNALING & PROTEIN BIOLOGY Small GTPase/G-Protein

Small GTPase / G-Protein Signaling
Small GTP-binding proteins (GTPases) regulate a variety of cell signaling pathways and
are therefore involved in a wide range of cell functions, processes, and morphology. The
most studied small GTPases include Ras, Rac, Rho and Cdc42. We offer a variety of
tools to enable the study of these small GTPase family members:
Small GTPase Activation Assays

Small GTPase Assay Beads
Active Rac-GEF Assay
Small GTPase Expression Vectors

Small GTPase Premade Adenoviruses
Small GTPase Retroviral Constructs
In addition, we offer sensitive assays to detect cyclic AMP and cyclic GMP, both of which
are important regulators in the G-Protein signaling cascade.
Small GTPase Activation Assays

Our Small GTPase Activation Assays use visible agarose beads to selectively pull down the active form of
the target of interest. The precipitated GTPase is then
detected by Western blot using a target specific antibody included in the kit.
Safe: Non-radioactive assay format

Visual Check: Agarose beads can be easily seen
Fast Results: 1 hour plus electrophoresis/blotting
If you are studying more than one small GTPase target, consider one of our Small GTPase Activation
Assay Combo Kits. These combo kits allow you to
measure the following targets at a savings compared
to buying separate kits for each target:
Rac1 and Cdc42

RhoA, Rac1 and Cdc42
Visible Agarose Beads Provided

in the Small GTPase Activation
Assays. Beads are easy to visualize, making it easier to avoid potential loss during washes and aspirations.
Small GTPase Activation Assay Principle.
98
Phone 1-858-271-6500
Fax 1-858-271-6514
Small GTPase/G-Protein CELL SIGNALING & PROTEIN BIOLOGY

Small GTPase Activation Assays (continued)
1. Geryk-Hall, M. et al. (2010). Driven to death: inhibition of farnesylation increases Ras activity in osteosarcoma and promotes growth arrest
and cell death. Mol. Cancer Ther. 9:1111-1119. (STA-400)
2. Camalier, C.E. et al. (2010). Elevated phosphate activates N-ras and promotes cell transformation and skin tumorigenesis. Cancer Prev.
Res. 3:359-370. (STA-400)
3. Harmon, B. et al (2008). Induction of the Gq signaling cascade by the human immunodeficiency virus envelope is required for virus entry.
J. Virol. 82:9191-9205. (STA-400)
4. Richier, L. et al. (2010). NOS1AP associates with scribble and regulates dendritic spine development. J. Neurosci. 30:4796-4805. (STA401-1)
5. Lise, M-F. et al. (2009). Myosin-Va-interacting protein, RILPL2, controls cell shape and neuronal morphogenesis via Rac signaling. J. Cell
Sci. 122:3810-3821. (STA-401-1)
6. Shen, E. et al. (2009). Rac1 is required for cardiomyocyte apoptosis during hyperglycemia. Diabetes 58:2386-2395. (STA-401-1)
7. Takano, A. et al. (2009). Identification of nectin-4 oncoprotein as a diagnostic and therapeutic target for lung cancer. Cancer Res. 69:66946703. (STA-401-1)
8. Zhang, S. et al (2008). The tumor suppressor LKB1 regulates lung cancer cell polarity by mediating Cdc42 recruitment and activity. Cancer
Res. 68:740-748. (STA-402)
9. Hayes, N.V.L. et al. (2010). Expression of neuroregulin 4 splice variants in normal human tissues and prostate cancer and their effects on
cell motility. Endocr. Relat. Cancer 18:39-49. (STA-403-A)
10. Godin, C.M. et al. (2010). The angiotensin II type 1 receptor induces membrane blebbing by coupling to RhoA, Rho kinase, and myosin
light chain kinase. Mol. Pharmacol. 77:903-912. (STA-403-A)
11. Chen, H. et al. (2010). Integrity of SOS1/EPS8/ABI1 tri-complex determines ovarian cancer metastasis. Cancer Res. 70:9979-9990. (STA403-A, STA-404)
12. Sultana, H. et al. (2010). Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes
scapularis ticks. J. Exp. Med. 10.1084/jem.20100276. (STA-404)
13. Pandey, D. et al. (2009). Unraveling a novel Rac1-mediated signaling pathway that regulates cofilin dephosphorylation and secretion in
thrombin stimulated platelets. Blood 114:415-424. (STA-404)
14. Baranwai, S. et al. (2011). Molecular characterization of the tumor-suppressive function of nischarin in breast cancer. J. Natl. Cancer Inst.
10.1093/jnci/djr350. (STA-405)
15. Tian, D. et al. (2010). Antagonistic regulation of actin dynamics and cell motility by TRPC5 and TRPC6 signals. Sci. Sig. 3:ra77. (STA-405)
16. Xu, Y. et al. (2010). Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. J. Cell Biol. 188:115-130. (STA405)
Product Name
Detection
Size
Catalog Number
Arf1 Activation Assay
Immunoblot/ECL
20 Assays
STA-407-1
Immunoblot/ECL
20 Assays
STA-407-6
Cdc42 Activation Assay
Immunoblot/ECL
20 Assays
STA-402
Rac1 Activation Assay
Immunoblot/ECL
20 Assays
STA-401-1
Rac2 Activation Assay
Immunoblot/ECL
20 Assays
STA-401-2
Ral Activation Assay
Immunoblot/ECL
20 Assays
STA-408
Ran Activation Assay
Immunoblot/ECL
20 Assays
STA-409
Rap1 Activation Assay
Immunoblot/ECL
20 Assays
STA-406-1
Immunoblot/ECL
20 Assays
STA-406-2
Pan-Ras Activation Assay
Immunoblot/ECL
20 Assays
STA-400
H-Ras Activation Assay
Immunoblot/ECL
20 Assays
STA-400-H
K-Ras Activation Assay
Immunoblot/ECL
20 Assays
STA-400-K
N-Ras Activation Assay
Immunoblot/ECL
20 Assays
STA-400-N
RhoA Activation Assay
Immunoblot/ECL
20 Assays
STA-403-A
RhoB Activation Assay
Immunoblot/ECL
20 Assays
STA-403-B
RhoC Activation Assay
Immunoblot/ECL
20 Assays
STA-403-C
Rac1/Cdc42 Activation Assay Combo Kit
Immunoblot/ECL
20 Assays/Target
STA-404
RhoA/Rac1/Cdc42 Activation Assay Combo Kit
Immunoblot/ECL
10 Assays/Target
STA-405
www.cellbiolabs.com
99

Active Rac-GEF Assay Kit (Tiam1)
Guanine nucleotide exchange factors (GEFs) activate small GTPases by catalyzing the exchange of GDP for
GTP.
Our Active Rac-GEF Assay Kit uses the agarose bead technology of our Small GTPase Activation Assays
(see previous page) to pull down the active form of Rac-GEFs from endogenous lysates or purified samples.
The specific GEF known as Tiam1 is then specifically detected with a polyclonal antibody.
Product Name
Active Rac-GEF Assay Kit (Tiam1)
Detection
Size
Catalog Number
Immunoblot/ECL
20 Assays
STA-422
Small GTPase and GEF Assay Beads

Our agarose beads are useful for selectively pulling
down only the active form of small GTPases. The
beads are colored for easily visualization. These
are the same beads used in our Small GTPase Activation Assays and Active Rac-GEF Assay Kit.
Visible Agarose Beads.

Beads are easy to visualize,
making it easier to avoid potential loss during washes
and aspirations.
Product Name
100

1. Moniz, S. et al (2007). Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2.
Oncogene 26(41):6071-6081. (STA-410)
2. Zhang, Q-G. et al. (2009). Estrogen attenuates ischemic oxidative damage via an estrogen receptor alpha-mediated inhibition
of NADPH oxidase activation. J. Neurosci. 29:13823-13836.
(STA-411)
3. Levy-Adam, F. et al. (2008). Heparanase facilitates cell adhesion and spreading by clustering of cell surface heparan sulfate
proteoglycans. PLoS ONE 3(6):e2319. (STA-411)
4. Sabbatini, M. E. et al. (2010). CCK activates RhoA and Rac1
differentially through G-alpha-13 and G-alpha-q in mouse pancreatic acini. Am. J. Physiol. Cell Physiol. 298:C592-C605. (STA
-411, STA-412)
5. Sabbatini, M. et al (2008). Rap1 activation plays a regulatory
role in pancreatic amylase secretion. J. Biol. Chem. 283:2388423894. (STA-412)
6. Colacios, C. et al. (2011). The p.Arg63Trp polymorphism controls Vav1 functions and Fox3p regulatory T cell development. J.
Exp. Med. 208:2183-2191. (STA-432)
Target
Size
Catalog Number
GGA3 PBD Agarose Beads
Arf
400 g
STA-419
PAK1 PBD Agarose Beads
Cdc42, Rac
400 g
STA-411
Raf1 RBD Agarose Beads
Ras
400 g
STA-410
RalBP1 PBD Agarose Beads
Ral
400 g
STA-420
RalGDS RBD Agarose Beads
Rap
400 g
STA-418
RanBP1 Agarose Beads
Ran
400 g
STA-421
Rhotekin RBD Agarose Beads
Rho
400 g
STA-412
Cdc42 G15A Agarose Beads
Cdc42-GEF
800 g
STA-433
Rac1 G15A Agarose Beads
Rac1-GEF
800 g
STA-432
RhoA G17A Agarose Beads
RhoA-GEF
400 g
STA-431
Phone 1-858-271-6500
Fax 1-858-271-6514

All of Cell Biolabs premade recombinant adenoviruses contain 5 x 109 viral particles per vial. They
are provided as 50 l aliquots at a concentration of
1 x 1011 viral particles/mL in TBS with 10% glycerol.
1. Black, S.A. et al (2008). TGF1 stimulates connective tissue
(ADV-150)
3. Rendon, B. et al. (2007). Regulation of human lung adenocarcinoma cell migration and invasion by MIF. J. Biol. Chem.
282:29910-29918. (ADV-150)
morphogenesis. J. Cell Sci. 122:929-936. (ADV-150, ADV-153,
ADV-154)
5. Cheng, Z.-J. et al. (2010). Co-regulation of caveolar and Cdc42
-dependent fluid phase endocytosis by phosphocaveolin-1. J.
Biol. Chem. 285:15119-15125. (ADV-153)
105:15399-15404. (ADV-156)
7. Vaught, D. et al. (2009). Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol.
Biol. Cell 20:2572-2581. (ADV-157)
J. Clin. Invest. 118:64-78. (ADV-157)
9. Fang, W.B. et al. (2008). Overexpression of EPHA2 receptor
Actin Cytoskeleton Staining.

Cos-7 cells were infected with
purified Ad-Ras V12 (ADV146) at 50 MOI (multiplicity of
infection). Membrane ruffling
was visualized by staining the
actin cytoskeleton with Rhodamine-coupled Phalloidin.
Target Name
Catalog Number
Cdc42
ADV-152
ADV-154
ADV-153
Rac1
ADV-149
ADV-151
ADV-150
ADV-145
ADV-146
Ras V12C40
ADV-148
Ras V12S35
ADV-147
ADV-157
ADV-156
Gene-Specific Recombinant Retroviral Vectors

Each vector is supplied as 100 L of bacterial
glycerol stock.
Generic Map of
pBABEhygro
Retroviral
Expression
Vector.
Target Name
Vector Backbone
Catalog Number
Cdc42 L61
pBABEhygro
RTV-203
K-Ras
pBABEpuro
RTV-220
K-Ras Q61
pWZLhygro
RTV-221
myr-Rac1
pBABEpuro
RTV-201
Rac1 V12
pBABEhygro
RTV-202
N-Ras K61
pBABEpuro
RTV-222
Rac3 V12
pBABEhygro
RTV-205
Ras V12
pBABEpuro
RTV-101
Ras V12C40
pBABEpuro
RTV-104
Ras V12G37
pBABEpuro
RTV-103
Ras V12S35
pBABEpuro
RTV-102
RhoA L63
pBABEhygro
RTV-204
www.cellbiolabs.com
101

Small GTPase Expression Vector Sets
Our Small GTPase Expression Vectors are ideal
tools for the study of the most commonly researched small GTPase targets. Each set contains
3 mammalian expression vectors:
Wild type
Dominant negative
Constitutively active
Vectors are supplied with or without a GFP reporter

gene.
Product Name
Vectors
Size
Catalog Number
Cdc42 Expression Vector Set
Wild Type, T17N (Dom. Neg.), Q61L (Active)
3 x 100 L
STA-455
GFP-Cdc42 Expression Vector Set
3 x 100 L
STA-451
Rac1 Expression Vector Set
Wild Type, T17N (Dom. Neg.), G12V (Active)
3 x 100 L
STA-454
GFP-Rac1 Expression Vector Set
3 x 100 L
STA-450
H-Ras Expression Vector Set
3 x 100 L
STA-457
RhoA Expression Vector Set
3 x 100 L
STA-456
GFP-RhoA Expression Vector Set
3 x 100 L
STA-452
Active Small GTPase Expression Vector Sets

Our Active Small GTPase Expression Vectors are similar to the expression vectors above, except that they are
provided as sets of 3 different active mutants for a single small GTPase target. Choose from Rac1 or H-Ras.
Product Name
Vectors
Size
Catalog Number
Active Rac1 Expression Vector Set
Q61L, Q61L/F37A, Q61L/Y40C
3 x 10 g
STA-458
Active H-Ras Expression Vector Set
V12, V12/S35, V12/C40
3 x 10 g
STA-459
Product Name
Size
Catalog Number
Exoenzyme C3 Expression Vector
10 g
STA-460
Exoenzyme C3 (Rho Inhibitor) Expression Vector
102
Phone 1-858-271-6500
Fax 1-858-271-6514

cAMP and cGMP ELISA Kits
Cyclic AMP and cyclic GMP are important regulatory
molecules in the GPCR signaling cascade. Our cAMP
and cGMP ELISA Kits provide a highly sensitive
method to measure low levels of cAMP or cGMP in a
variety of sample types.
2.5
OD 450nm
Sensitive: Detect as little as 1 pmol/mL

Versatile: Suitable for use with cell and tissue lysates, urine, plasma, or culture medium
Convenient: Strip-well plate format with either
colorimetric or chemiluminescent detection
1.5
0.5

1. Chen, M. et al (2010). Involvement of cAMP in nerve growth
factor-triggered p35/Cdk5 activation and differentiation in PC12
cells. Am. J. Physiol. Cell Physiol. 299:C516-C527. (STA-500)
2. McCoy, K.L. et al. (2010). PAR1 and PAR2 couple to overlapping and distinct sets of G proteins and linked signaling pathways to differentially regulate cell physiology. Mol. Pharmacol.
77:1005-1015. (STA-500)
0.0
0.1
1.0
10.0
100.0
1000.0
10000.0 100000.0
cAMP (pmol/mL)
Standard Curve Created with the cAMP ELISA Kit, Colorimetric

Format.
Product Name
Detection
Size
Catalog Number
96 Assays
STA-500
5 x 96 Assays
STA-500-5
96 Assays
STA-501
5 x 96 Assays
STA-501-5
96 Assays
STA-505
5 x 96 Assays
STA-505-5
96 Assays
STA-506
5 x 96 Assays
STA-506-5
Colorimetric
cAMP ELISA Kit
Chemiluminescent
Colorimetric
cGMP ELISA Kit
Chemiluminescent
Recombinant GRP-PH Domain
This recombinant protein is expressed and purified

from E. coli as a fusion protein, and is provided at
1.0 mg/ml in 1X PBS.
2.5
PIP2
PIP3
2
OD 450nm
The GRP1 (general receptor for phosphoinositide)

protein binds phosphatidylinositol-3,4,5triphosphate (PIP3) through a pleckstrin homology
(PH) domain and displays a region of high sequence similarity to the yeast Sec7 protein.
1.5
PIP3 Binding
to GRP-PH
Domain.
1
0.5
0
0
20
40
Amount (ng)
Product Name
Size
Catalog Number
100 g
STA-200
1 mg
STA-200-1MG
Recombinant GRP-PH Domain
www.cellbiolabs.com
103
CELL SIGNALING & PROTEIN BIOLOGY Kinase Assays

Rho Kinase (ROCK) Activity Assays
Rho Kinase (ROCK) is a serine/threonine kinase which is a target of Rho. ROCK mediates Rho signaling and
reorganizes the actin cytoskeleton via the phosphorylation of several substrates that contribute to contractility
and the assembly of actin filaments.
Our ROCK Activity Assays provide a non-radioactive
format to measure the level of active ROCK in cell or
tissue lysates. The immunoblot kit provides a convenient format for measuring ROCK activity in a few samples, while the 96-well Activity Assay contains a stripwell plate precoated with MYPT1 for higher throughput.
Results Using the ROCK Activity Immunoblot Kit. Lanes 1, 3,

5, 7: Without ROCK (negative control). Lanes 2, 4, 6, 8: With
ROCK. Lanes 1 & 2: 200 ng MYPT1; Lanes 3 & 4: 100 ng;
Lanes 5 & 6: 50 ng; Lanes 7 & 8: 25 ng. Phosphorylation of
MYPT1 substrate was detected by anti-phospho-MYPT1 as described in the protocol.
1. Wang, J.N. et al. (2011). Response gene to complement 32 promotes vascular lesion formation through stimulation of smooth
muscle cell proliferation and migration. Arterioscler. Thromb. Vasc.
Biol. 31:e19-e26. (STA-415)
2. Li, Z. et al. (2009). TrkBT1 induces liver metastasis of pancreatic
cancer cells by sequestering Rho GDP dissociation inhibitor and
promoting RhoA activation. Cancer Res. 69:7851-7859. (STA-415)
3. Xiao, L. et al. (2009). ROCK mediates phorbol ester-induced
apoptosis in prostate cancer cells via p21-Cip1 upregulation and
JNK. J. Biol. Chem. 284:29365-29375. (STA-415)
4. Burger, D. et al. (2011). Endothelial microparticle formation by
angiotensin II is mediated via ang II receptor type I/NADPH oxidase/Rho kinase pathways targeted to lipid rafts. Arterioscler.
Thromb. Vasc. Biol. 31:1898-1907. (STA-416)
5. Haas, B. et al. (2009). Protein kinase G controls brown fat cell
differentiation and mitochondrial biogenesis. Sci. Signal. 2:ra78.
(STA-416)
Product Name
104
96-Well ROCK Activity Assay Principle.

Detection
Size
Catalog Number
ROCK Activity Immunoblot Kit
Immunoblot
20 Assays
STA-415
96 Assays
STA-416
96-Well ROCK Activity Assay
Colorimetric
5 x 96 Assays
STA-416-5
Phone 1-858-271-6500
Fax 1-858-271-6514
Kinase Assays CELL SIGNALING & PROTEIN BIOLOGY

Checkpoint Kinase Activity Assays
Checkpoint kinases, including CHK1 and CHK2, can
be activated in response to DNA damage prior to mitosis. These kinases phosphorylate Cdc25C, a protein phosphatase, at Ser-216. This phosphorylation
ultimately leads to cell cycle arrest, preventing mitosis
and avoiding the passage of DNA damage to daughter cells.
Our Checkpoint Kinase Activity Assays allow you to
conveniently measure the activity of CHK1 and
CHK2. The assays use recombinant Cdc25C as a
checkpoint kinase substrate. Phosphorylated
Cdc25C (Ser216) is detected using a phosphospecific antibody. Checkpoint Kinase Activitiy Assays are available in two formats: a Western blot
assay and a 96-well plate-based activity assay.
CHK1 Activity Using the Checkpoint Kinase Activity

Immunoblot Kit. Lane 1: Negative Control; Lane 2: 10
ng of active CHK1.
Product Name
96-Well Checkpoint Kinase Activity Assay Principle.

Detection
Size
Catalog Number
Checkpoint Kinase Activity Immunoblot Kit
Immunoblot
20 Assays
STA-413
96 Assays
STA-414
96-Well Checkpoint Kinase Activity Assay Kit
Colorimetric
5 x 96 Assays
STA-414-5
www.cellbiolabs.com
105
CELL SIGNALING & PROTEIN BIOLOGY Reporter Molecules

Reporter Assays, Cell Lines and Reagents
We offer a variety of tools for various reporter molecules:
Reporter Assays
Reporter Stable Cell Lines
Antibodies to Reporter Molecules

Reporter Viral Vectors
-Galactosidase Staining Kit

LacZ is a commonly used reporter gene in transfection experiments because its gene product, -galactosidase,
is extremely stable and resistant to proteolytic degradation, making it easy to assay.
Our -Galactosidase Staining Kit provides an efficient, easy-to-use method to determine the transfection efficiency of the LacZ gene. Each kit provides sufficient reagents to perform 75 assays in 35mm culture dishes.
Black, S.A. et al. (2008). TGF1 stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through
a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGF1-induced CCN2/CTGF expression. J. Biol.
Chem. 283:10835-10847. (AKR-100)
Product Name
-Galactosidase Staining Kit
Size
Catalog Number
75 Assays
AKR-100
GFP Quantitation Kits

Most imaging studies of rGFP are qualitative, and
quantitation by FACS is time consuming and expensive. Our GFP Quantitation Kit measures GFP fluorescence in a fluorometer, while our GFP ELISA kit
uses a standard microplate reader.
Sensitive: Detection limit of 30 pg/ml with
ELISA format
Easy Quantitation: Measure GFP levels in a
fluorometer or a standard microplate reader
1. Wamboldt, Y. et al. (2009). Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation
in higher plants. Plant Cell 21:157-167. (AKR-120)
2. Coghill, J.M. et al. (2010). Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CCchemokine receptor 7 on donor T cells. Blood 115:4914-4922.
(AKR-121)
3. Rajan, S. et al. (2010). In vitro processing and secretion of mutant insulin proteins that cause permanent neonatal diabetes.
Am. J. Physiol. Endocrinol. Metab. 298:E403-E410. (AKR-121)
4. Glenn, S.T. et al. (2008). In vivo analysis of key elements within
the renin regulatory region. Physiol. Genomics 35:243-253.
(AKR-121)
106
Standard Curve Generated with the GFP ELISA Kit.
Product Name
Detection
Size
Catalog Number
GFP ELISA Kit
Colorimetric
96 Assays
AKR-121
GFP Quantitation Kit
Fluorometric
100 Assays
AKR-120
Phone 1-858-271-6500
Fax 1-858-271-6514
Reporter Molecules CELL SIGNALING & PROTEIN BIOLOGY

Reporter Stable Cell Lines
These cells stably express one or more fluorescent proteins. All cell lines are provided as 1 x 106 cells in 1 mL.
Cell Line
Catalog Number
Cell Line
Catalog Number
Cell Line
Catalog Number
293/CFP
AKR-270
HEY/GFP
AKR-205
MDA-MB-468/GFP
AKR-204
293/GFP
AKR-200
MCF-7/GFP
AKR-211
NIH3T3/GFP
AKR-214
293/Luc
AKR-230
MCF-7/Luc
AKR-234
OVCA429/GFP
AKR-212
293/YFP
AKR-280
MDA-MB-231/GFP
AKR-201
OVCAR-5/RFP
AKR-254
293T/GFP-Puro
AKR-202
MDA-MB-231/GFP-RFP
AKR-221
SKOV-3/GFP-Luc
AKR-225
A549/GFP
AKR-209
MDA-MB-231/Luc
AKR-231
SKOV-3/Luc
AKR-232
BT-549/GFP
AKR-255
MDA-MB-231/RFP
AKR-251
SKOV-3/RFP
AKR-253
ES-2/GFP
AKR-206
MDA-MB-436/GFP
AKR-203
T47D/GFP
AKR-208
HeLa/GFP
AKR-213
MDA-MB-436/RFP
AKR-252
ZR-75-1/GFP
AKR-210
Recombinant EGFP
Recombinant EGFP is provided at a concentration of 1 mg/mL and includes a 6xHis-tag at the C-terminus.
Product Name
Size
Catalog Number
100 g
STA-201
5 x 100 g
STA-201-5
100 g
STA-202
5 x 100 g
STA-202-5
Recombinant EGFP
Recombinant RFP
Monoclonal Antibodies to Reporter Molecules

Antibodies are provided at a concentration of 1 mg/mL. GFP antibody also recognizes EGFP, YFP, EYFP
and CFP. RFP antibody recognizes Tag-RFP, Turbo-RFP, DeRed, mCherry and mOrange. Antibodies
are suitable for Western blot, Immunostaining, ELISA and Dot blot.
Product Name
Size
Catalog Number
Mouse Anti-GFP Monoclonal Antibody (clone GF28R)
100 g
AKR-020
Mouse Anti-RFP Monoclonal Antibody (clone RF5R)
100 g
AKR-021
Reporter Viral Vectors

Premade viruses and viral constructs with reporter molecules make ideal controls for viral gene delivery experiments. Please see the following pages for these reporter viral vectors:
Adeno-Associated Virus, p. 38
Adenovirus, p. 42
Lentivirus, p. 51
Retroviral Constructs, p. 56
www.cellbiolabs.com
107
CELL SIGNALING & PROTEIN BIOLOGY Epitope Tags

Monoclonal Antibodies to Epitope Tags
Antibodies are provided at a concentration of 1 mg/mL. All are suitable for Western blot, Immunostaining,
ELISA, Immunoprecipitation, and Dot blot.
Product Name
Size
Catalog Number
Mouse Anti-FLAG Tag Monoclonal Antibody (clone FG4R)
100 g
AKR-004
Mouse Anti-GST Tag Monoclonal Antibody (clone GST.B6)
100 g
AKR-005
Mouse Anti-HA Tag Monoclonal Antibody (clone HA.C5)
100 g
AKR-006
Mouse Anti-His Tag Monoclonal Antibody (clone HIS.H8)
100 g
AKR-003
Mouse Anti-Myc Tag Monoclonal Antibody (clone Myc.A7)
100 g
AKR-007
Mouse Anti-V5 Tag Monoclonal Antibody (clone E10)
100 g
AKR-008
His-Tag Protein ELISA Kit
Sensitive: Detect as little as 1 ng/mL protein or 50

pM of 6xHis-tag residues
Versatile: Use with proteins containing His-tag at
either N- or C-terminus
2.000
1.800
1.600
1.400
OD 450nm
Our His-Tag Protein ELISA Kit allows you to detect

and quantify His-tagged protein samples simply and
reliably by comparing unknown samples with a recombinant standard. The kit is suitable for use with
cell and tissue lysates.
1.200
1.000
0.800
0.600
0.400
0.200
0.000
0.00
0.01
0.10
1.00
10.00
100.00
N-Terminal His-Tag Protein Standard (g/mL)
Quantitation of N-Terminal His-Tag Protein.

Product Name
His-Tag Protein ELISA Kit
Size
Catalog Number
96 assays
AKR-130
Rapid GST Inclusion Body Solubilization and Renaturation Kit

Pre Beads Post Beads
Recombinant proteins expressed in bacteria often form

inclusion bodies, especially when expressed at high
levels. The Rapid GST Inclusion Body Solubilization
and Renaturation Kit is designed to retrieve expressed
GST fusion proteins in soluble form after lysis and extraction. Each kit contains sufficient reagents to solubilize and renature up to 5-10 liters of bacterial culture.
GS Beads
1 2 3 4 5 6 7 8 9 10 11 12 13 14
Fast Results: No lengthy dialysis or dilution steps

Easy-to-Use: No pH variation or redox pair
Optimized: Designed specifically to solubilize and
renature GST inclusion bodies
Sabbah, M. et al. (2011). CCN5, a novel transcriptional repressor
of transforming growth factor- signaling pathway. Mol. Cell Biol.
31:1459-1469.
108
Solubilization and Renaturation of GST-RTK Fusion Protein.

Lane 1: MW STD; Lane 2: Whole E.Coli lysate; Lane 3, 7, 11: No
detergent; Lane 4, 8, 12: 32-fold dilution; Lane 5, 9, 13: 8-fold dilution; Lane 6, 10, 14: 2-fold dilution.
Product Name
Size
Catalog Number
Rapid GST Inclusion Body Solubilization and Renaturation Kit
1 Kit
AKR-110
Phone 1-858-271-6500
Fax 1-858-271-6514
Protein Phosphorylation CELL SIGNALING & PROTEIN BIOLOGY

PhosphoBLOCKER Western Blot Blocking Reagent
Western blot blockers such as dry milk or serum are
sufficient to block unreactive sites on the membrane.
However, they are not designed to preserve phosphoprotein antigens during blotting.
1. Kasuboski, J.M. et al. (2011). Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on
kinetochores. Mol. Cell Biol. 22:3318.3330.
2. Ishii, K. et al. (2010). Insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm
Bombyx mori. J. Biol. Chem. 285:28635-28642.
3. Song, A. et al. (2010). A C. elegans elF4E-family member
upregulates translation at elevated temperatures of mRNAs
coding MSH-5 and other meiotic crossover proteins. J. Cell Sci.
123:2228-2237.
4. Ramakrishnan, R. et al. (2009). Characterization of Cdk9 T-loop
phosphorylation in resting and activated CD4+ T lymphocytes. J.
Leukoc. Biol. 86:1345-1350.
High Sensitivity: Enhances low level phosphoprotein signal without increasing background
Easy-to-use: Premixed dry blend
Dry Milk
PhosphoBLOCKER Reagent
Superior Blocking with PhosphoBLOCKER Reagent. A549
cell lysate was blocked with dry milk or PhosphoBLOCKER before
detection with anti-Phospho-p38 antibody.
Product Name
Size
Catalog Number
1L
AKR-103
4L
AKR-104
PhosphoBLOCKER Western Blot Blocking Reagent
Phospho Antibody Stripping Solution

This solution removes anti-phosphoantibodies selectively from blots without significantly affecting the
immobilized proteins, allowing re-probing of the blot
with the same or a different antibody. Stripping of
antibodies is done at room temperature, so no heating of blots is required.
Product Name
Phospho Antibody Stripping Solution
Multiple Blotting
and Stripping of
4G10 Phosphotyrosine Antibody.
Size
Catalog Number
10 mL
AKR-102
Phosphoprotein Purification Kit

Our Phosphoprotein Purification Kit allows you to
enrich your phosphoprotein samples quickly and
easily. Phosphorylated proteins are affinity purified
from lysates with a single-step purification / enrichment matrix. The entire procedure takes about 4
hours. Each prep can process 2.5 mg of total lysate
protein, or approximately one confluent 10 cm dish.
Product Name
Enrichment of p-ERK.
HeLa cell lysate was
incubated with the Phosphoprotein Enrichment
Matrix from the Phosphoprotein Purification
Kit.
Size
Catalog Number
2 preps
AKR-105
5 preps
AKR-106
Phosphoprotein Purification Kit
www.cellbiolabs.com
109
CELL SIGNALING & PROTEIN BIOLOGY Antibody Production

Antibody Isotyping Kits
Our Antibody Isotyping Kits provide a quick, convenient method to determine the class and/or subclass
of your monoclonal antibody. Kits use a simple lateral flow format and contain two or three cassettes:
IgG1, IgG2a and IgG2b

IgG3, IgA and IgM
(optional) Kappa and Lambda light chains
Mouse Antibody Isotyping

Results from Two Different
Samples. Left: IgA isotype.
Right: IgG2a isotype.
Kits combine the speed and convenience of the lateral flow format with the sensitivity of an ELISA.
Fast: Read results in 5 to 10 minutes
Versatile: Suitable for use with both mouse ascites
fluid and tissue culture supernatants
Product Name
Detection
Mouse Antibody Isotyping Kit
Size
Catalog Number
5 Assays
AKR-150
10 Assays
AKR-151
5 Assays
AKR-152
10 Assays
AKR-153
Lateral Flow
Mouse Antibody Isotyping Kit, with Kappa & Lambda Light Chains
Lateral Flow
Rapid Antibody Purification Kit

Our Rapid Antibody Purification Kit is designed for
fast, single-step purification of high-quality IgG from
ascites, serum, tissue culture media or hybridoma
supernatants. IgG-containing samples are incubated
with immobilized Protein A in the presence of a binding buffer. Non-IgG components are washed and IgG
is subsequently eluted. The supplied Protein A column is suitable for 10 purification preps. The capacity per prep depends on the species of IgG; examples are listed in the column at right.
Species
mg of IgG per Prep
Bovine
15-20
Goat
6-12
Human
20-30
Mouse
6-12
Rabbit
15-20
Capacity per Prep for the Rapid Antibody Purification Kit.
Product Name
Rapid Antibody Purification Kit
Size
Catalog Number
10 Preps
AKR-160
Loading Control Antibodies

Antibodies are provided at a concentration of 1 mg/mL and are suitable for Western blot, Immunostaining,
ELISA, Immunoprecipitation, and Dot blot.
Product Name
110
Size
Catalog Number
Mouse Anti-GAPDH Monoclonal Antibody
100 g
AKR-001
Mouse Anti--Actin Monoclonal Antibody
100 g
AKR-002
Phone 1-858-271-6500
Fax 1-858-271-6514
METABOLISM RESEARCH
Lipoprotein Metabolism
Lipoprotein Metabolism Research

Lipoproteins are important assemblies of proteins and lipids that have implications in cardiovascular and related diseases. We offer a variety of kits and reagents for the study of
this important area of metabolism research:
Apolipoprotein ELISA Kits

ApoAI / ApoB Duplex Assay
OxLDL ELISA Kit
Cholesterol Assays
Lipoproteins and Apolipoproteins
Antibodies to Apolipoproteins
Human Apolipoprotein ELISA Kits

Apolipoproteins comprise the protein component of
lipoprotein assemblies. Apolipoproteins fall into two
classes: non-exchangeable (ApoB-100 and ApoB-48)
and exchangeable (AI, AII, CI, CII, CIII, and E).
Apo
AII
Apo
B
Apo
CI
Apo
CII
Apo
CIII
Apo
E
50
pg/mL
0.3
ng/mL
1
ng/mL
200
pg/mL
1
ng/mL
50
pg/mL
200
pg/mL
Our Human Apo ELISA Kits provide a convenient and

sensitive method for quantifying specific apolipoproteins in serum, plasma, or other biological fluids.
Detection Limits of Cell Biolabs Human Apo ELISA Kits.
Structure of LDL or HDL.
Human ApoB ELISA Standard Curve.
Product Name
112
Apo
AI
Detection
Size
Catalog Number
Human ApoAI ELISA Kit
Colorimetric
96 Assays
STA-362
Human ApoAII ELISA Kit
Colorimetric
96 Assays
STA-363
Human ApoB ELISA Kit
Colorimetric
96 Assays
STA-368
Human ApoCI ELISA Kit
Colorimetric
96 Assays
STA-364
Human ApoCII ELISA Kit
Colorimetric
96 Assays
STA-365
Human ApoCIII ELISA Kit
Colorimetric
96 Assays
STA-366
Human ApoE ELISA Kit
Colorimetric
96 Assays
STA-367
Phone 1-858-271-6500
Fax 1-858-271-6514
METABOLISM RESEARCH
Human ApoAI and ApoB Duplex ELISA Kit

As the primary protein components of HDL and LDL
respectively, ApoAI and ApoB are arguably the most
significant apolipoproteins in lipid metabolism research.
Our Human ApoAI and ApoB Duplex ELISA Kit provides a convenient tool to quantify both proteins in a
single serum or plasma sample. Unlike other multiplex assays, our ApoAI and ApoB Duplex ELISA
does not require a luminometer for detection. Simply
quantify both proteins sequentially using a standard
colorimetric ELISA plate reader.
Human ApoAI and ApoB Duplex ELISA Kit Assay Principle.

Both ApoAI and ApoB are captured individually from a single serum
or plasma sample. A mixture of primary antibodies is added, followed by a mixture of enzyme conjugates. AP Substrate is added
to quantify ApoAI; followed by the addition of HRP Substrate to
quantify ApoB.
Product Name
Human ApoAI and ApoB Duplex ELISA Kit
Efficient: Quantify two apolipoproteins from the

same sample in just a few hours
Sensitive: Detect as little as 0.1 ng/mL of ApoAI
and 1 ng/mL of ApoB from serum or plasma
Quantitative: Compare results to known ApoAI and
ApoB standards
ApoAI and ApoB Standard Curves Generated Using the

Human ApoAI and ApoB Duplex ELISA Kit.
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-361
www.cellbiolabs.com
113
METABOLISM RESEARCH
OxiSelect Human Oxidized LDL ELISA

Low density lipoprotein (LDL) contains a hydrophobic
core of various lipids surrounded by one molecule of
Apolipoprotein B-100 (ApoB-100), which promotes
solubility of the LDL in blood. LDL is often described
as bad cholesterol, but it is even more dangerous in
the human body when it becomes oxidized. Oxidized
LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner lining of arteries.
The majority of oxidized residues in LDL are
malondialdehyde (MDA) adducts.

Quantitative: Compare unknown samples with
provided copper oxidized LDL standard
Our OxiSelect Human Oxidized LDL ELISA Kit is

designed for the detection and quantitation of MDAmodified LDL in human plasma or serum.

Oxidized LDL ELISA Kit.
OxiSelect Human Oxidized LDL ELISA Assay Principle.

Product Name
OxiSelect Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation)
114
Phone 1-858-271-6500
Quantitation of OxLDL in Serum and Plasma Samples. Serum

and plasma samples were treated with LDL Precipitation Solution.
Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further dilution 1:160 in Assay Diluent according to the Assay
Protocol.
Detection
Size
Catalog Number
Colorimetric
96 Assays
STA-369
Fax 1-858-271-6514
METABOLISM RESEARCH
Total Cholesterol Assay Kit
Cholesterol exists within lipoproteins in two forms: a
free alcohol and a fatty cholesteryl ester, which is the
predominant form of cholesterol transport and storage. Our Total Cholesterol Assay Kit is a simple
fluorometric assay that measures the amount of cholesterol in serum, plasma, cell lysates or tissue homogenates. In the presence of cholesterol esterase,
the assay will measure total cholesterol in both forms.
In the absence of the esterase, the assay will measure only free cholesterol.
5000
Sensitive: Detect as little as 100 nM

Fast: Simple 30 minute protocol
Plasma
4500
Serum
4000
RFUs
3500
3000
2500
2000
1500
1000
500
0
1 to 10
1 to 100
1 to 1000
Serum and Plasma Dilutions
1 to 10000
Cholesterol Values from Normal Human Serum and Plasma

Samples. Relative Fluorescence Unit (RFU) values are then compared against a Cholesterol Standard Curve (not shown).
Assay Principle for the Total Cholesterol Assay Kit.
Product Name
Total Cholesterol Assay Kit
Detection
Size
Catalog Number
Fluorometric
192 Assays
STA-390
HDL and LDL/VLDL Cholesterol Assay Kit
In the presence of cholesterol esterase, the assay will

measure total cholesterol in both free cholesterol and
cholesteryl ester forms. In the absence of the esterase, the assay will measure only free cholesterol.
Quantitation of cholesteryl ester only may be calculated by subtracting free cholesterol from total cholesterol levels.
Product Name
HDL and LDL/VLDL Cholesterol Assay Kit
8000
7037
6000
RFU
Our HDL and LDL/VLDL Cholesterol Assay Kit is

similar in principle to our Total Cholesterol Assay Kit,
but allows you to quantify HDL and LDL/VLDL separately in serum samples. After separating samples
into HDL and LDL/VLDL fractions, the fluorometric
assay is run according to the Assay Principle for the
Total Cholesterol Assay Kit shown above.
TotalCholesterol
(HDL+LDL/VLDL)
5011
LDL/VLDL
HDL
4000
1769
2000
0
1
Quantitation of Total Cholesterol, LDL/VLDL, and HDL.
Detection
Size
Catalog Number
Fluorometric
192 Assays
STA-391
www.cellbiolabs.com
115
METABOLISM RESEARCH
Apolipoproteins and Lipoproteins

Product Name
Size
Catalog Number
Human Albumin
100 g
STA-230
Human Albumin, Malondialdehyde Modified
100 g
STA-210
Human Apolipoprotein AI
100 g
STA-232
Human Apolipoprotein AII
100 g
STA-233
Human Apolipoprotein B-100
100 g
STA-234
Human Apolipoprotein B-100, Malondialdehyde Modified
100 g
STA-211
Human Apolipoprotein CI
100 g
STA-235
Human Apolipoprotein CIII
100 g
STA-237
Human C-Reactive Protein
100 g
STA-240
Human High Density Lipoprotein (HDL)
100 g
STA-243
Human High Density Lipoprotein-2 (HDL-2)
100 g
STA-244
Human High Density Lipoprotein-3 (HDL-3)
100 g
STA-245
Human Low Density Lipoprotein (LDL)
100 g
STA-241
Human Low Density Lipoprotein (LDL), Copper (Cu++) Oxidized
100 g
STA-214
Human Low Density Lipoprotein (LDL), Malondialdehyde Modified
100 g
STA-212
Human Low Density Lipoprotein (LDL), Nitrated
100 g
STA-213
Human Plasminogen
100 g
STA-239
Human Very Low Density Lipoprotein (VLDL)
100 g
STA-242
Detection
Size
Catalog Number
Goat Anti-Human Albumin Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-130
Sheep Anti-Human Apolipoprotein (a) Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-131
Goat Anti-Human Apolipoprotein AI Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-132
Rabbit Anti-Human Apolipoprotein AII Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-133
Goat Anti-Human Apolipoprotein B-100/48 Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-134
Rabbit Anti-Human Apolipoprotein CI Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-135
Rabbit Anti-Human Apolipoprotein CII Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-136
Rabbit Anti-Human Apolipoprotein CIII Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-137
Goat Anti-Human Apolipoprotein E Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-138
Rabbit Anti-Human C-Reactive Protein Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-140
Goat Anti-Human Plasminogen Polyclonal Antibody
Immunoblot/ELISA
100 g
STA-139
Affinity Purified Antibodies to Apolipoproteins

Product Name
116
Phone 1-858-271-6500
Fax 1-858-271-6514
Renal Function Assays
METABOLISM RESEARCH

Kidney Injury Molecule-1 (KIM-1) is a well-published marker for kidney tubular toxicity.
Creatinine is well-known as an indicator of glomerular filtration rate. Our Renal Function
Assays provide a simple, sensitive method to test for these markers of kidney function.
OxiSelect Kidney Injury Molecule-1 (KIM-1) Assays

KIM-1 is an excellent marker of kidney tubular toxicity. It also has been shown to associate with spatial
expression of nitrotyrosine and inducible nitric oxide
synthase.
Our OxiSelect KIM-1 Assays are useful for detecting this marker in urine. For quick results choose
one of our lateral flow detection (dipstick) kits. Our
KIM-1 ELISA kits provide unsurpassed results when
sensitivity is critical.
Color Comparison Chart for the OxiSelect Human KIM-1

Lateral Flow Detection Kit.
Product Name
OxiSelect KIM-1 Assays are available for either

human or rat urine samples in two formats:
Highly Sensitive ELISA Format: Detect concentrations as low 300 pg/mL
Ultra-Fast Lateral Flow Format: Obtain results in
15 minutes

KIM-1 ELISA Kit. Concentrations are expressed in ng/mL.
Detection
Size
Catalog Number
OxiSelect Human KIM-1 ELISA Kit
Colorimetric
96 Assays
STA-374
10 Assays
STA-370
OxiSelect Human KIM-1 Lateral Flow Detection Kit
Lateral Flow
50 Assays
STA-371
96 Assays
STA-376
10 Assays
STA-372
50 Assays
STA-373
OxiSelect Rat Kim-1 ELISA Kit
Colorimetric
OxiSelect Rat Kim-1 Lateral Flow Detection Kit
Lateral Flow
www.cellbiolabs.com
117
METABOLISM RESEARCH
Creatinine Assay Kit
2.5
2
1.5
1
0.5
0
0
10
15
Creatinine(mg/dl)
1
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
Product Name
Creatinine Assay Kit
118
Phone 1-858-271-6500
25
Without
Glucose
With
Glucose
Serum
Mechanism of Creatinine Production from Creatine and
Phosphocreatine.
20
Standard Curve Generated with the Creatinine Assay Kit.
OD490nm
Our Creatinine Assay Kit is based on the Jaffe reaction between creatinine and alkaline picrate, which
produces an orange-red color complex that can be
easily read by a standard microplate reader at 490
nm. A creatinine standard is provided to allow quantitative measurements of creatinine levels in urine,
serum, cell lysates, and tissue homogenates. The
assay is extremely simple and takes less than one
hour to perform.
OD490nm
Creatinine is a metabolite formed from creatine and

phosphocreatine (p-creatine). Subsequently
creatinine enters the blood and is then excreted by
the kidneys via glomerular filtration. Intra-individual
variation of creatinine levels is <15% daily, making it
a useful marker for normalizing levels of other molecules found in the urine.
Urine
Creatinine Levels in Urine and Serum Samples in the Presence and Absence of Glucose.
Detection
Size
Catalog Number
Colorimetric
200 Assays
STA-378
Fax 1-858-271-6514
INFECTIOUS DISEASE RESEARCH
Viral Core Antigens
Virus Core Antigen Detection

Core antigens provide a convenient way to detect and quantify certain infectious viruses.
We offer a variety of ELISA kits to measure the core antigen levels in plasma, serum, or
purified virus preps.
Note: All assays are for research use only; they are not to be used for diagnostic purposes.
QuickTiter p24 ELISA Kits (HIV-1 and FIV)

Fast: Read results in a few hours
Versatile: Suitable for use with native virus or recombinant lentiviral supernatants
3
2.5
OD 450 nm
A popular method of quantifying HIV-1 or FIV is the

p24 ELISA. Our p24 ELISA kits provide a quick, convenient way to quantify the concentration of your lentivirus. Individual kits are available to measure either
HIV-1 or FIV concentrations.
2
1.5
1
0.5
0
0
25
50
75
100
FIV p24 (ng/mL)

Standard Curve Generated with the QuickTiter FIV Lentivirus
Quantitation Kit.
Product Name
Detection
QuickTiter HIV Lentivirus Quantitation Kit (HIV-1 p24 ELISA)
QuickTiter FIV Lentivirus Quantitation Kit (FIV p24 ELISA)
Size
Catalog Number
96 Assays
VPK-108-H
5 x 96 Assays
VPK-108-H-5
96 Assays
VPK-108-F
5 x 96 Assays
VPK-108-F-5
Colorimetric
Colorimetric
QuickTiter FeLV Core Antigen ELISA Kit

Feline leukemia virus (FeLV) is a retrovirus or oncornavirus. Our QuickTiter FeLV Core Antigen ELISA
Kit specifically measures the level of the p27 core
antigen in blood or other samples.
Product Name
QuickTiter FeLV Core Antigen ELISA Kit (FeLV p27)
120
Phone 1-858-271-6500
Sensitive: Detect as little as 100 pg/mL

Fast: Read results in a few hours
Fully quantitative: Recombinant core antigen included as positive control
Detection
Size
Catalog Number
Colorimetric
96 Assays
VPK-155
Fax 1-858-271-6514
Viral Core Antigens
QuickTiter HBV and HCV Core Antigen ELISA Kits

These QuickTiter kits specifically quantify the core
antigens of Hepatitis B and Hepatitis C viruses, respectively. A mouse monoclonal antibody is coated
onto an 8 x 12 strip-well plate which allows the flexibility to save some of the wells for later use.

Fast: Results in about 5-6 hours
Fully Quantitative: Recombinant core antigen
included as positive control
3.5
2.5
2.5
OD 450nm
OD 450nm
1.5
2
1.5
1
1
0.5
0.5
0
0
20
40
60
80
100
120
HBVcAg p24 (ng/mL)
20
40
60
80
100
120
HCVcAg p24 (ng/mL)
Standard Curve Generated with the QuickTiter HBV Core

Antigen ELISA Kit.
Product Name
Standard Curve Generated with the QuickTiter HCV Core

Antigen ELISA Kit.
Detection
Size
Catalog Number
QuickTiter HBV Core Antigen ELISA Kit
Colorimetric
96 Assays
VPK-150
QuickTiter HCV Core Antigen ELISA Kit
Colorimetric
96 Assays
VPK-151
QuickTiter MuLV Core Antigen ELISA Kit

Murine leukemia virus (MuLV) is a retrovirus capable of causing cancer in mice and related vertebrates. Recently discovered in humans, xenotropic murine leukemia virus-related virus (XMRV) is closely related to MuLV;
the p30 core antigens share 96% identity.
Our QuickTiter FeLV Core Antigen ELISA Kit specifically measures the level of the p30 core antigen in
blood or other samples.
Product Name
QuickTiter MuLV Core Antigen ELISA Kit (MuLV p30)
Sensitive: Detect as little as 300 pg/mL

Fully quantitative: Recombinant core antigen included as positive control
Detection
Size
Catalog Number
Colorimetric
96 Assays
VPK-156
www.cellbiolabs.com
121
Bacterial Rapid Detection
Rapid Qualitative E. Coli and Salmonella Tests

Our Rapid Tests for E. coli and Salmonella provide a
quick and easy method for detection of these pathogens in contaminated food samples.
The kits use a convenient strip method that gives a
clear positive or negative result. Samples are enriched overnight in the presence of enrichment medium, then incubated for 10-20 minutes with the test
strip. A control line is provided to ensure the test is
valid.
Example of Positive and Negative Results Using the E. coli or
Salmonella Rapid Test Kit.
122
Product Name
Detection
Size
Catalog Number
Rapid Qualitative E. coli O157:H7 Test Kit
Test Strip
20 Assays
AKR-301
Rapid Qualitative Salmonella Test Kit
Test Strip
20 Assays
AKR-302
Phone 1-858-271-6500
Fax 1-858-271-6514
PRODUCT INDEXALPHABETICAL
Product
293 Cell Lines
AAV
Adenovirus
GFP Stable Expression
Lentivirus
Retrovirus
4-HNE
Antibodies
ELISA Kit
6-4PP Quantitation Kits
8-Iso-Prostaglandin F2
ELISA
8-Isoprostane ELISA Kit
8-OHdG ELISA Kit
8-OHG ELISA Kit
A549/GFP Cell Line
AAV (Adeno-Assoc. Virus)
Cell Line
Expression Systems
Expression Vectors
Helper Free Systems
Packaging Systems
Premade Control Viruses
Purification Kit
Quantitation Kit
Titer Kit
Transduction Reagent
Active Rac-GEF Assay
Adenovirus
Cell Line
Expression Systems
Premade Recombinant
Purification Kits
Quantitation Kits
RCA Assay
Titer Kits
Transduction Reagent
Adhesion Assays
Advanced Glycation End
Products Assay
Advanced Oxidation Protein
Products Assay
AGE Assays
Albumin
Antibody
Protein
Alkaline Phosphatase Assays
Angiogenesis
Recombinant Adenovirus
Tube Formation Assay
Anoikis Assays
Antibodies
Albumin
Apolipoproteins
Beta-Actin
Carboxymethyl Lysine (CML)
C-Reactive Protein
Page
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Product
Antibodies (contd)
Flag Tag
Fluorescent Proteins
GAPDH
GFP
GST Tag
HA Tag
His Tag
HNE (4-Hydroxynonenal)
MDA (Malondialdehyde)
Methylglyoxal (MG)
Myc Tag
Nitrotyrosine
Plasminogen
RFP
Antibody Tools
Blocking Reagent
Isotyping Kits
Production Tools
Purification Kit
Western Stripping Solution
Antioxidant Assays
Catalase Activity Assay
HORAC Assay
ORAC Assay
Superoxide Dismutase
Activity Assay
AOPP Assay
AP Sites Quantitation Kit
Apo AI ELISA
Apo AI/Apo B Duplex ELISA
Apo AII ELISA
Apo B ELISA
Apo CI ELISA
Apo CII ELISA
Apo CIII ELISA
Apo E ELISA
Apolipoproteins
Antibodies
ELISA Kits
Proteins
AUF1 Retroviral Vector
Autophagy Expression
Vectors
-Actin Antibody
-Galactosidase
Recombinant Lentivirus
Reporter Assays
Bacterial Rapid Test Kits
Biochips for Cell Adhesion
Blocking Reagent
BPDE
DNA Adduct ELISA
Protein Adduct ELISA
BT-549/GFP Cell Line
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98-99
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80
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Product
C3 Expression Vector
CA9 Recomb. Adenovirus
c-Abl Retroviral Vector
cAMP ELISA Kits
Cancer Cell Assays
Angiogenesis
Anoikis
Cell Adhesion
Cell Invasion
Cell Migration
Cell Transformation
Colony Formation
Soft Agar
Tumor Cell Isolation Kit
Tumor Sensitivity
Carbonyl Assays
Carboxyethyl Lysine ELISA
Carboxymethyl Lysine
Assays
Catalase Activity Assays
Cdc42
Activation Assay
Agarose Beads
Retroviral Vector
CEA Recomb. Adenovirus
CEL ELISA Kit
Cell-Based Assays
Adhesion
Angiogenesis
Anoikis
Cell Contraction
Cell Viability
Chemotaxis
Colony Formation
Cytotoxicity
Haptotaxis
Invasion
Migration
Phagocytosis
Senescence
Soft Agar
Transformation
Transmigration
Tumor Sensitivity
Wound Healing
Cell Cycle
Adenoviruses
Anoikis Assay
Cell Viability Assay
Cytotoxicity Assay
Retroviral Vectors
Senescence Assays
Cell Invasion Assays
Cell Lines
293AAV
293AD
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10-11
18-19
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Product
Cell Lines (contd)
293LTV
293RTV
293/CFP
293/GFP
293/Luc
293/YFP
293T/GFP-Puro
A549/GFP
BT-549/GFP
ES-2/GFP
HeLa/GFP
HEY/GFP
JK1 Feeder Cells
MCF-7/GFP
MCF-7/Luc
MDA-MB-231/GFP
MDA-MB-231/GFP-RFP
MDA-MB-231/Luc
MDA-MB-231/RFP
MDA-MB-436/GFP
MDA-MB-436/RFP
MDA-MB-468/GFP
MEF Feeder Cells
NIH3T3/GFP
OVCA429/GFP
OVCAR-5/RFP
Plat-A Retroviral Packaging
Cells
Plat-E Retroviral Packaging
Cells
Plat-GP Retroviral Packaging
Cells
SKOV-3/GFP-Luc
SKOV-3/Luc
SKOV-3/RFP
SNL Feeder Cells
T47D/GFP
ZR-75-1/GFP
Cell Migration Assays
Cell Transformation Assays
Cell Viability Assay
cGMP ELISA Kits
Checkpoint Kinase Assays
Chemotaxis Assays
Cholesterol Assays
Clonogenic Tumor Cell
Isolation Kit
CML (Carboxymethyl Lysine)
Antibodies
Assays
c-Myc Retroviral Vectors
Hematopoietic Colony
Forming Cell Assay
Stem Cell Colony Assay
124
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107
60-61
60-61
60-61
107
107
107
29
107
107
12-19
6-7
22
103
105
15, 19
115
9
79
79
57
6-7
Phone 1-858-271-6500
30
31
Product
(contd)
Tumor Sensitivity Assay
Comet Assay Kits & Slides
Core Antigen Assays
CPD Quantitation Kits
C-Reactive Protein
Antibody
Protein
Cre Recombinant Adenovirus
Creatinine Assay
CSK Recombinant
Adenovirus
Cyclic AMP ELISA Kits
Cyclic GMP ELISA Kits
CytoSelect Cell-Based
Assays
Anoikis
Cell Adhesion
Cell Invasion
Cell Migration
Cell Transformation
Cell Viability
Chemotaxis
Colony Formation
Cytotoxicity
Haptotaxis
Phagocytosis
Soft Agar
Transmigration
Tumor Sensitivity
Wound Healing
Cytoskeleton Regulation
Activation Assays
Adenoviruses
Expression Vectors
Retroviral Vectors
Cytotoxicity Assay
DCC Recombinant
Adenovirus
DNA Damage Assays
8-OHdG ELISA Kit
AP Sites Quantitation Kit
BPDE Adduct
Comet Assays
Double-Strand Break Assay
UV Damage
DNA Hydroxymethylation
Assay
DNA Methylation Assays
E. coli Rapid Test Kit
ECM (Extracellular Matrix)
Kits
Endothelial Tube Assay
Page
9
8
87
120-1
89
116
116
42
118
45
103
103
22
10-11
18-19
12-19
6-7
22
15, 19
6-9
22
16
21
6-9
17
8
20
98-99
43
102
57
22
43
86
88
90
87
88
89
91
91
122
10
18-19
24
24
Product
ERK2
Retroviral Vector
ERK5 Recombinant
Adenovirus
ES/EC Cells
Alkaline Phosphatase Assays
Systems
ES-2/GFP Cell Line
Exoenzyme C3 Expression
Vector
Extracellular Matrix Kits
Feeder Cells
FeLV p27 Core Antigen ELISA
Firefly Luciferase Recombinant Adenovirus
FIV p24 ELISA
Flag Tag Antibody
Fyn Recombinant Adenovirus
Gap Closure Migration
Assays
GAPDH Antibody
GEF (Guanine Exchange
Factors)
Activation Assays
Agarose Beads
GFP
Antibody
ELISA Kit
Lentiviral Vectors
Quantitation Kits
Recombinant Protein
Retroviral Vectors
Stable Cell Lines
GGA3 Agarose Beads
Global DNA Methylation
Assays
Glutathione Assay
GPCR Signaling Products
GST
Antibody
Inclusion Body Solubilization
and Renaturation Kit
GTPase Assay Kits
HA Tag Antibody
Haptotaxis Assays
HBV Core Antigen ELISA
HCV Core Antigen ELISA
HDL
Assay
Lipoprotein, Human
Page
44
58
44
32
31
28
107
102
10
18-19
24
29
120
42
120
108
45
13
110
100
100
107
106
51
106
42
51
107
56
107
100
91
95
103
108
108
98-99
108
14
121
121
115
116
Fax 1-858-271-6514
Product
HEK 293 Cell Lines
AAV
Adenovirus
GFP Stable Expression
Lentivirus
Retrovirus
HeLa/GFP Cell Line
Forming Cell Assay
Hepatitis B Core Antigen
ELISA
Hepatitis C Core Antigen
ELISA
HEY/GFP Cell Line
HIF-1 Recomb. Adenovirus
High Density Lipoprotein
Assay
Protein
His Tag
Antibody
Protein ELISA
HIV-1 p24 ELISA Kits
HNE
Antibodies
Assays
hnRNPA0 Retroviral Vector
HORAC Assay Kit
H-Ras Activation Assay
HuB Retroviral Vector
HuC Retroviral Vector
HuD Retroviral Vector
HuR Retroviral Vector
Hydrogen Peroxide Assays
Hydroxyl Radical Antioxidant Capacity Assay
Hydroxymethylation Assay
IFN Recombinant Adenovirus
IB Recombinant Adenovirus
IKK Recombinant Adenovirus
IL-2 Recombinant Adenovirus
Immunoblot Blocking
Reagent
In Vitro Angiogenesis Assay
In Vitro Tumor Sensitivity
Assay
Inclusion Body Solubilization
Induced Pluripotent Stem
Cells
Lentiviral Vectors
Retroviral Packaging Cells
Retroviral Vectors
Invasion Assays
Lentiviral Vectors
Retroviral Packaging Cells
Retroviral Vectors
JK1 Feeder Cells
Page
39
42
107
50
59
107
30
121
121
107
42
115
116
108
108
52-53,
120
82
82
58
96
98-99
58
58
58
58
93
96
91
44
45
45
44
109
24
8
108
27
26
26
18-19
27
26
26
29
Product
JNK1
Retroviral Vector
Klf4 Retroviral Vectors
Kidney Injury Molecule-1
(KIM-1) Assays
KOSM Lentiviral Vector
K-Ras Activation Assay
LC3 Expression Vectors
LDL
Assay
Lipoprotein, Human
Oxidized
Lentivirus
Cell Line
Concentration & Purification
Kits
Control Plasmids
Expression Systems
Expression Vectors
Packaging Systems
Premade Control Viruses
Purification Kits
Quantitation Kits
Titer Kits
Transduction Kits
Leukocyte Assays
Adhesion
Transmigration
Lin-28 Retroviral Vectors
Lipid Peroxidation Assays
8-Isoprostane ELISA
HNE Adduct ELISA
Malondialdehyde (MDA)
Assays
TBARS Assay
Lipoproteins
Antibodies
Assays
Human
Oxidized
Luciferase
Reporter Cell Lines
Malondialdehyde
Antibodies
Assays
Retroviral Vectors
MAPKAPK2
Retroviral Vector
MCF-7/GFP Cell Line
MCF-7/Luc Cell Line
MDA (Malondialdehyde)
Antibodies
Assays
Page
44
58
57
81,
117
27
98-99
24
115
116
116
50
54
51
49-50
51
50
51
54
52-53
52-53
55
11
17
57
83
82
84
83
116
112-5
116
116
42
107
84
83-84
44
58
44
58
107
107
84
83-84
www.cellbiolabs.com
Product
MDA-MB-231/GFP Cell Line
MDA-MB-231/Luc Cell Line
MDA-MB-231/RFP Cell Line
MDA-MB-436/RFP Cell Line
MEF Feeder Cells
MEK1
Retroviral Vector
MEK5 Recomb. Adenovirus
MEKK1 Recomb. Adenovirus
MEKK3 Recomb. Adenovirus
Methylglyoxal (MG)
Antibody
ELISA Kit
Microfluidic Biochips
microRNA Analysis
System
Clone Collection
Control Vectors
Expression Vectors
Functional Reporter System
Lentiviral Expression System
Precursor Clone Collection
Retroviral Expression Vector
Transduction Enhancer
Migration Assays
miRNA Analysis
System
Clone Collection
Control Vectors
Expression Vectors
Functional Reporter System
Lentiviral Expression System
Precursor Clone Collection
Retroviral Expression Vector
Transduction Enhancer
MKK3
Retroviral Vector
MKK4 Recomb. Adenovirus
MKK6
Retroviral Vector
MKK7 Recomb. Adenovirus
MuLV p30 Core Antigen
ELISA
Myc Tag Antibody
MyoD Recomb. Adenovirus
Myogenin Adenovirus
myr-Akt Retroviral Vectors
myr-Rac1
Retroviral Vector
NANOG Retroviral Vectors
Page
107
107
107
107
107
107
29
44
58
44
44
44
79
79
11
73
66-71
72
66-72
72
73
66-71
74
74
12-19
73
66-71
72
66-72
72
73
66-71
74
74
44
58
44
44
58
44
121
108
43
43
58
44
57
57
125
Product
N-Carboxyethyl Lysine
ELISA
N-Carboxymethyl Lysine
Antibody
Assay Kits
NFB Adenoviruses
NIH3T3/GFP Cell Line
Nitrated LDL
Nitrotyrosine
Antibodies
Assay Kits
NOD2 Recombinant
Adenovirus
N-Ras Activation Assay
NY-ESO-1 Adenovirus
Oct-3/4 Retroviral Vectors
ORAC Assay Kit
OVCA429/GFP Cell Line
OVCAR-5/RFP Cell Line
Oxidized LDL
Assay Kit
Lipoprotein, Human
Oxidized Proteins
OxiSelect Oxidative
Stress / Damage Assays
Antioxidant Assays
DNA / RNA Damage Kits
Lipid Peroxidation Assays
Protein Oxidation Assays
ROS Assays
OxLDL Assay
Oxygen Radical Antioxidant
Capacity (ORAC) Assay
p24 ELISA Kits
p38
Retroviral Vectors
p53
Lentiviral Vectors (shRNA)
Retroviral Vectors
p68 RNA Helicase Adenovirus
PABP Retroviral Vector
PAK1
PBD Agarose Beads
Peroxide Detection Assays
Phagocytosis Assays
Phospho Antibody Stripping
Solution
PhosphoBLOCKER
Western Blot Blocking
Reagent
Phosphoproteins
Antibody Stripping Solution
126
Page
79
79
79
45
107
81
77
77
45
98-99
42
57
96
107
107
85,
114
81
81
94-96
86-91
82-85
76-81
92-93
85,
114
96
52-53,
120
44
58
43
27
56
43
58
100
43
93
21
109
109
109
Phone 1-858-271-6500
Product
Phosphoproteins (contd)
Blocking Reagent
Purification Kit
PI3K Retroviral Vector
PKC Recombinant
Adenovirus
Plasminogen
Antibody
Protein, Human
Plat-A Retroviral Packaging
Cells
Plat-E Retroviral Packaging
Cells
Plat-GP Retroviral Packaging
Cells
Platinum Retroviral
Expression
Expression Systems
Packaging Cell Lines
PRAK
Retroviral Vector
Protease Retroviral Vectors
Protein Oxidation Assays
Advanced Glycation End
Products (AGE)
Advanced Oxidation Protein
Products (AOPP)
BPDE Adduct
Carbonyl
CEL (Carboxyethyl Lysine)
CML (Carboxymethyl Lysine)
MG (Methylglyoxal)
Nitrotyrosine
Protein Phosphorylation
Antibody Stripping Solution
Blocking Reagent
Purification Kit
Proteins
Albumin
Apolipoproteins
EGFP
GRP-PH Domain
Oxidized/Nitrated
Purification Kits
AAV
Adenovirus
Antibodies
Lentivirus
Phosphoproteins
Retrovirus
QuickTiter Viral Quantitation & Titer Kits
AAV
Adenovirus
FeLV p27
FIV p24
HBV Core Antigen
Page
109
109
58
45
116
116
60-61
60-61
60-61
60-61
61
44
58
57
79
80
80
78
79
79
79
77
109
109
109
116
116
107
103
81
39
46
110
54
109
62
40
47
120
120
121
Product
QuickTiter Viral Quantitation & Titer Kits (contd)
HCV Core Antigen
HIV p24
Lentivirus, Recombinant
MuLV p30
Retrovirus, Recombinant
Rac
Activation Assay
Agarose Beads
GEF Assay
Retroviral Vectors
Radius Cell Migration
Assays
Raf1
Retroviral Vectors
Ral
Activation Assay
Agarose Beads
Ran
Activation Assay
Agarose Beads
RAPAd Adenoviral
Expression Systems
Rapid GST Inclusion Body
Solubilization and Renaturation Kit
Rapid RCA Assay
Ras Superfamily
Activation Assays
Agarose Beads
Expression Vectors
Retroviral Vectors
RCA Assay Kit
Reactive Oxygen Species
(ROS) Assays
Recombinant Adenoviruses
Recombinant Proteins
Fluorescent Proteins
GRP-PH Domain
Rel B Recombinant
Adenovirus
Reporter Genes
Lentiviral Vectors
Quantitation Assays
Retroviral Vectors
Stable Cell Lines
Retrovirus
Concentration & Purification
Kits
Page
121
120
52-53
121
63
98-99
100
100
43
57
13
44
58
98-99
100
98-99
100
98-99
98-99
41, 73
108
48
98-99
100
102
43
57
48
92-93
42-45
107
103
45
117-8
51
106
42
51
56
107
62
Fax 1-858-271-6514
Product
Retrovirus (contd)
Expression Systems
Expression Vectors
Gene-Specific Vectors
Packaging Cell Lines
Purification Kits
Quantitation Kits
Transduction Kits
RFP
Antibody
Stable Cell Lines
Rho
Activation Assays
Agarose Beads
Retroviral Vector
RhoA Activation Assay
RhoB Activation Assay
RhoC Activation Assay
Rho Kinase Activity Assays
RNA Damage ELISA Kit
RNAi Enhancer Reagent
ROCK Activity Assay Kits
ROS Assays
Salmonella Rapid Test Kit
SCGE Assay Kits
SEAP Recombinant
Adenovirus
Senescence Assays
shAkt Recombinant
Adenovirus
Single Cell Gel Electrophoresis Assays
SKOV-3/GFP-Luc Cell Line
SKOV-3/Luc Cell Line
SKOV-3/RFP Cell Line
Small GTPase
Activation Assays
Active GEF Assays
Agarose Beads
Expression Vectors
Premade Adenoviruses
Retroviral Vectors
SNL Feeder Cells
SOD Activity Assay Kit
Soft Agar Colony Assay Kits
Hematopoietic Colony Forming Cell Assay
Stem Cell Colony Formation
Assay
Tumor Sensitivity Assay
SOK Recombinant
Adenovirus
Sox2 Retroviral Vector
Page
60-61
59
56-58
59-61
62
63
64
107
107
98-99
100
43
57
98-99
98-99
98-99
104
86
74
104
92-93
122
87
42
23
45
87
107
107
107
98-99
100
100
102
43
57
29
94
6-7
30
31
9
8
44
57
Product
Src Recombinant Adenovirus
Stat5 Retroviral Vectors
Stem Cell Research
Alkaline Phosphatase
Detection Kits
Feeder Cells
Forming Cell Assay
PCR Primers
Systems
Stem Cell Colony Assay
Total Protein: ES Cell Line D3
Total RNA: ES Cell Line D3
Superoxide Dismutase Assay
T47D/GFP Cell Line
TAC Assay
Tac-Rac1 Adenovirus
TBARS Assay Kit
TIA1 Retroviral Vector
Tiam1 Assay Kit
TIAR Retroviral Vector
Total Antioxidant Capacity
Assay
Total Cholesterol Assay
Total Protein: ES Cell Line D3
Total RNA: ES Cell Line D3
Transcription Regulation
Retroviral Vectors
Transformation Assays
Transmigration Assays
TTP Retroviral Vector
Tumor Antigen Adenoviruses
Tumor Cell Assays
Cell Adhesion
Cell Invasion
Cell Migration
Cell Transformation
Chemosensitivity
Soft Agar Colony Formation
Transmigration
Tumor Cell Isolation
Tyrosine Kinase
Adenoviruses
uPA / uPAR Retroviral
Vectors
UV DNA Damage Assays
V5 Tag Antibody
VEGF Recombinant
Adenovirus
Very Low Density Lipoprotein
Assay
Lipoprotein, Human
ViraBind Purification Kits
AAV
Adenovirus
Page
45
58
32
29
30
26-27
32
28
31
32
32
94
107
95
44
83
58
100
58
95
115
32
32
58
6-7
17
58
24
42
10-11
18-19
12-19
6-7
8
6-9
17
9
www.cellbiolabs.com
45
57
89
108
42
115
116
39
46
Product
Page
ViraBind Purification Kits
(contd)
Lentivirus
54
Retrovirus
62
ViraDuctin Transduction
Kits & Reagents
AAV
40
Adenovirus
48
Lentivirus
55
Retrovirus
64
AAV
35-37
Adenovirus
41
Lentivirus
49-50
Retrovirus
60-61
Viral Packaging Cells
AAV
39
Adenovirus
42
Lentivirus
50
Retrovirus
60-61
Viral Titer Kits
AAV
40
Adenovirus
47
Lentivirus
52-53
Retrovirus
63
Viral Transduction Reagents
AAV
40
Adenovirus
48
Lentivirus
55
Retrovirus
64
ViraSafe Lentivirus
49-51,
Expression Systems
73
Virus Core Antigen Assays
FeLV p27
120
FIV p24
120
HBVcAg
121
HCVcAg
121
HIV-1 p24
120
MuLV p30
121
Virus Purification Kits
AAV
39
Adenovirus
46
Lentivirus
54
Retrovirus
62
Virus Quantitation Kits
AAV
40
Adenovirus
47
Lentivirus
52-53
Retrovirus
63
VLDL
Assay
115
Lipoprotein, Human
116
VSV-G Retroviral Vector
59
Western Blot Blocking
109
Reagent
Wound Healing Assay
20
ZR-75-1/GFP Cell Line
107
127
PRODUCT INDEXCATALOG NO.

Catalog No.
AAV-100
AAV-200
AAV-201
AAV-301
through
AAV-402
AD-100
AD-200
AD-201
ADV-001
through
ADV-008
ADV-100
ADV-101
ADV-102
through
ADV-164
ADV-202
through
ADV-210
ADV-301
through
ADV-418
ADV-501
through
ADV-509
ADV-601
through
ADV-604
AKR-001
AKR-002
AKR-003
through
AKR-008
AKR-020
AKR-021
AKR-100
AKR-102
through
AKR-106
AKR-110
AKR-120
AKR-121
AKR-130
AKR-150
through
AKR-160
AKR-200
through
AKR-280
AKR-301
AKR-302
CBA-003
through
CBA-004-5
CBA-050
through
CBA-071
CBA-080
128
Page
39
40
40
38
42
48
48
42
42
42
44
43
45
43
42
110
110
108
107
107
106
109
108
106
106
108
110
107
122
122
11
10
22
Catalog No.
Page
CBA-081
22
CBA-100
15
CBA-100-C
19
CBA-100-COL 16
CBA-100-FN
16
CBA-101
15
CBA-101-C
19
CBA-101-COL 16
CBA-101-FN
16
CBA-102
through
15
CBA-106
CBA-106-C
19
CBA-120
20
CBA-120-5
20
CBA-125
through
13
CBA-127-5
CBA-130
6
CBA-130-5
6
CBA-135
7
CBA-135-5
7
CBA-140
7
CBA-140-5
7
CBA-145
7
CBA-145-5
7
CBA-150
8
CBA-150-5
8
CBA-155
9
CBA-155-5
9
CBA-200
24
CBA-201
23
CBA-210
11
CBA-211
11
CBA-212
17
CBA-215
11
CBA-216
17
CBA-220
21
CBA-224
21
CBA-230
23
CBA-231
23
CBA-232
23
CBA-240
22
CBA-300
32
CBA-301
32
CBA-302
32
CBA-303
32
CBA-304
32
CBA-305
32
CBA-307
32
CBA-308
32
CBA-310
29
CBA-311
29
CBA-312
29
CBA-313
29
CBA-315
29
CBA-316
29
Phone 1-858-271-6500
Catalog No.
Page
CBA-320
30
CBA-320-5
30
CBA-325
31
CBA-325-5
31
CBA-401
24
LTV-100
50
LTV-200
55
LTV-201
55
LTV-300
51
LTV-301
51
LTV-302
51
LTV-400
51
LTV-401
51
LTV-402
51
LTV-403
51
LTV-451
27
LTV-700
27
LTV-801
24
MIR-1-1
through
66
MIR-30C-2
MIR-30D
through
67
MIR-152
MIR-154
through
68
MIR-302B
MIR-302C
through
69
MIR-491
MIR-499
through
70
MIR-671
MIR-708
through
71
MIR-942
MIR-EXP-C
72
MIR-EXP-GP-C 72
MIR-GFP
72
MIR-LET7A-2
through
66
MIR-LET7I
MIR-NULL
72
MIR-NULL-GP
72
MMU-LET7A-1
through
66
MMU-LET7G
MMU-MIR-1-1
through MMU66
MIR-30C-1
MMU-MIR-30C
-2 through
67
MMU-MIR-151
MMU-MIR-152
through MMU68
MIR-302A
MMU-MIR302B through
69
MMU-MIR-497
Catalog No. Page

MMU-MIR-499
through
70
MMU-MIR-675
MMU-MIR-676
through
71
MMU-MIR1907
RNAI-200
74
RNAI-201
74
RTV-001
59
RTV-002
56
RTV-003
59
RTV-004
59
RTV-005
56
RTV-006
56
RTV-007
56
RTV-010
through
59
RTV-041
RTV-050
through
56
RTV-053
RTV-101
through
57
RTV-104
RTV-105
through
58
RTV-125
RTV-201
through
57
RTV-222
RTV-301
through
58
RTV-340
RTV-400
26
RTV-401
through
56
RTV-405
RTV-410
26
RTV-501
through
57
RTV-712
RTV-801
24
RV-100
59
RV-101
61
RV-102
61
RV-103
61
RV-110
through
59
RV-114
RV-200
64
RV-201
64
STA-003
77
STA-004
77
STA-011
79
STA-013
79
STA-014
79
Catalog No.
STA-031
STA-032
STA-034
STA-035
STA-130
STA-131
STA-132
STA-133
STA-134
STA-135
STA-136
STA-137
STA-138
STA-139
STA-140
STA-200
STA-2001MG
STA-201
STA-201-5
STA-202
STA-202-5
STA-210
STA-211
STA-212
STA-213
STA-214
STA-230
STA-232
STA-233
STA-234
STA-235
STA-237
STA-239
STA-240
STA-241
STA-242
STA-243
STA-244
STA-245
STA-300
STA-301
STA-303
STA-304
STA-305
STA-305-5
STA-307
STA-308
STA-309
STA-310
STA-310-5
STA-311
STA-312
STA-313
STA-314
STA-315
STA-316
STA-317
Page
84
84
82
82
116
116
116
116
116
116
116
116
116
116
116
103
103
107
107
107
107
116
116
116
116
116
116
116
116
116
116
116
116
116
116
116
116
116
116
79
80
77
77
77
77
78
78
78
78
78
79
95
79
79
78
79
79
Fax 1-858-271-6514
PRODUCT INDEXCATALOG NO.

Catalog No.
STA-318
STA-319
STA-320
STA-320-5
STA-321
STA-322
STA-322-5
STA-322-C
STA-323
STA-324
STA-325
STA-325-5
STA-326
STA-327
STA-328
STA-329
STA-330
STA-330-5
STA-331
STA-332
STA-332-5
STA-333
STA-334
STA-334-5
STA-335
STA-337
STA-337-5
STA-338
STA-338-5
STA-339
STA-340
STA-341
STA-342
STA-343
STA-344
STA-345
STA-345-5
STA-346
STA-346-5
STA-347
STA-350
STA-351
STA-351-5
STA-352
STA-353
STA-353-5
STA-354
STA-355
STA-355-5
STA-356
STA-356-5
STA-357
STA-360
STA-361
STA-362
STA-363
STA-364
Page
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Catalog No.
STA-365
STA-366
STA-367
STA-368
STA-369
STA-370
STA-371
STA-372
STA-373
STA-374
STA-376
STA-378
STA-380
STA-380-5
STA-381
STA-390
STA-391
STA-400
STA-400-H
STA-400-K
STA-400-N
STA-401-1
STA-401-2
STA-402
STA-403-A
STA-403-B
STA-403-C
STA-404
STA-405
STA-406-1
STA-406-2
STA-407-1
STA-407-6
STA-408
STA-409
STA-410
STA-411
STA-412
STA-413
STA-414
STA-414-5
STA-415
STA-416
STA-416-5
STA-418
STA-419
STA-420
STA-421
STA-422
STA-431
STA-432
STA-433
STA-450
STA-451
STA-452
STA-454
STA-455
STA-456
Page
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102
102
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102
Catalog No. Page

STA-457
102
STA-458
102
STA-459
102
STA-460
102
STA-500
103
STA-500-5
103
STA-501
103
STA-501-5
103
STA-505
103
STA-505-5
103
STA-506
103
STA-506-5
103
VPK-090
54
VPK-091
54
VPK-091-5
54
VPK-095
54
VPK-096
54
VPK-096-5
54
VPK-099
46
VPK-100
46
VPK-106
47
VPK-107
53
VPK-107-5
53
VPK-108-F
53
VPK-108-F-5
53
VPK-108-H
53
VPK-108-H-5 53
VPK-109
47
VPK-110
47
VPK-111
48
VPK-111-5
48
VPK-112
53
VPK-120
63
VPK-130
62
VPK-131
62
VPK-131-5
62
VPK-135
62
VPK-136
62
VPK-136-5
62
VPK-140
39
VPK-141
39
VPK-141-5
39
VPK-145
40
VPK-150
121
VPK-151
121
VPK-155
120
VPK-156
121
VPK-205
50
VPK-206
50
VPK-211
51
VPK-21150
ECO
VPK-21150
PAN
VPK-212
51
VPK-21250
ECO
www.cellbiolabs.com
Catalog No.
VPK-212PAN
VPK-213
VPK-213ECO
VPK-213PAN
VPK-214
VPK-214ECO
VPK-214PAN
VPK-215
VPK-215ECO
VPK-215PAN
VPK-216
VPK-216ECO
VPK-216PAN
VPK-217
VPK-217ECO
VPK-217PAN
VPK-218
VPK-218ECO
VPK-218PAN
VPK-219
VPK-219ECO
VPK-219PAN
VPK-220
VPK-220ECO
VPK-220PAN
VPK-250
VPK-251
VPK-252
VPK-253
VPK-254
VPK-300
VPK-301
VPK-302
VPK-303
VPK-304
VPK-305
VPK-306
VPK-307
VPK-308
VPK-400-DJ
VPK-400DJ-8
VPK-401
Page
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50
51
50
50
41
41
41
41
41
61
61
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61
61
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61
38
38
Catalog No.
VPK-402
VPK-403
VPK-404
VPK-405
VPK-406
VPK-410
VPK-410-DJ
VPK-410DJ-8
VPK-410SER1
through
VPK-410SER6
VPK-415
VPK-415-DJ
VPK-415DJ-8
VPK-415SER1
through
VPK-415SER6
VPK-416
VPK-416-DJ
VPK-416DJ-8
VPK-416SER1
through
VPK-416SER6
VPK-417
VPK-417-DJ
VPK-417DJ-8
VPK-417SER1
through
VPK-417SER6
VPK-418
VPK-418-DJ
VPK-418DJ-8
VPK-418SER1
through
VPK-418SER6
VPK-419
VPK-419-DJ
VPK-419DJ-8
VPK-419SER1
through
VPK-419SER6
Page
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129
WORLDWIDE CONTACTS
North America
UNITED STATES
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Cell Biolabs, Inc.

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JAPAN
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Genbiotech S.R.L.
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130
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Tel: 82-2-579-8787
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WORLDWIDE CONTACTS
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BELGIUM
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BULGARIA
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CROATIA
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Upstream OU
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LITHUANIA
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Tel: +233-54-349-5200
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NIGERIA
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LUXEMBOURG
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SPAIN
bioNova Cientifica S.L.
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SOUTH AFRICA
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MACEDONIA
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Tel: 0030 6974711158
jng@otenet.gr
SWEDEN
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TABLE OF CONTENTS

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Colony Formation Assays

Tumor Cell / Soft Agar Assays

CytoSelect 96-Well Cell Transformation AssayTraditional

Fast Results: 6-8 days vs. 21 days

With this assay, cells are incubated in a semisolid

CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony

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Colony Formation Assays

CytoSelect 96-Well Cell Transformation AssaysAdvanced

Faster Results: 6-8 days vs. 21 days

Cell Transformation Assay Principle. Cell colonies form after a 6

Recent Product Citations

CytoSelect 384-Well Cell Transformation Assay**

Colony Formation Assays

CytoSelect 96-Well In Vitro Tumor Sensitivity Assay

Tumor Sensitivity Assay Principle.

Log [5-FU] (M)

CytoSelect 96-Well In Vitro Tumor Sensitivity Assay

Inhibition of HeLa Cell Transformation by 5-Fluorouracil. HeLa

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Colony Formation Assays

CytoSelect Clonogenic Tumor Cell Isolation Kit

Efficient: Easily eliminates single cells from

Clonogenic Colony Formation, Isolation and Re-plating. A:

Clonogenic Tumor Cell Isolation Procedure.

Cell Adhesion Assays

CytoSelect ECM Cell Adhesion Assays

CytoSelect 48-Well Cell Adhesion Assay, Collagen I

CytoSelect 48-Well Cell Adhesion Assay, Collagen IV

CytoSelect 48-Well Cell Adhesion Assay, Fibrinogen

CytoSelect 48-Well Cell Adhesion Assay, Fibronectin

CytoSelect 48-Well Cell Adhesion Assay, Laminin

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CytoSelect Leukocyte Endothelium Adhesion Assays

Recent Product Citations

CytoSelect Leukocyte-endothelium Adhesion Assay