Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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com.83%. et al. Laviolette F. 520). 25: 955–964. 518) as the closest neighbors of strain HA. 5. 2009. 2.asm. strain HA is available in GenBank under accession number AJXD00000000. 65:1147–1154.asm. Acinetobacter sp. 32:277– 280. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. which can thus contribute to insect resistance (1). Lowe TM. and a GⴙC content of 41%. HA represents a genome size of 3. 518). A. 2010.555 reads) that led to ⬃45-fold coverage of the entire genome. Kajla MK. However. Corbeil J. Nucleic Acids Res.085 bp) of this strain that consists of 102 contigs. Nucleic Acids Res. 1997. 4. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. the presence of genes coding for esterase enzyme have been observed. 3. U. Four copies of 5S rRNA. Biol. Acinetobacter baumannii AB0057 (score. Hattori M. acknowledge the Council of Scientific and Industrial Research. 7.a Udita Mukherjee. A. 17:1519 –1533.. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. Brévault T. Lagesen K.b and Rup Lala Department of Zoology.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. A total of 2.pone. http://www. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. 2004.1371/journal. 2. Sixtyfour predicted tRNA genes. Here. New Delhi. The draft genome of Acinetobacter sp. India (7). Ojha A. doi:10. Manag. 2 copies of 23S rRNA.1128/JB. Khurana.. and N. we report the draft genome sequence (3.nih . and toxic compounds were found. HA will further 5156 jb. In addition. 2012.a Naseer Sangwan. 35:3100 –3108. The final assembly was validated using paired-end constraints (N50 contigs. for providing research fellowships. BMC Genomics 9:75. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. University of Delhi South Campus.a Raman Rajagopal.a Ankita Dua. The draft genome was annotated using RAST version 4. cinetobacter sp.ncbi. Here. a key component in carbohydrate metabolism. were identified by using tRNAscan-SE (6)..911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation.html).S. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0. University of Delhi. RAST results also indicated Acinetobacter lwoffii SH145 (score. PLoS One 7:e30768. Ghogomu RT.0. J.nlm. 511. on September Nucleotide sequence accession number. 2007. Detailed analysis of the genome sequence of Acinetobacter sp. Priya NG.125. and the KEGG database (4).. Comput.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology..4 kb).GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. Nucleic Acids Res.M. Strain HA.085 bp with an average G⫹C content of 41%. Boisvert S. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. Martin T. p. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal.D.0030768. 6.911 predicted coding sequences.S.a Paramjit Khurana. Achaleke J. Government of India. J. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. Raj A. N. 2012 by guest A . Aziz RK. Eddy SR. Pest.a Neeti Pandey. were found. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin.3 set at a k-mer length of 25 (3).a Anjali Saxena. The genome sequence of Acinetobacter sp. University Grants Commission. Also. Host plant induced variation in gut bacteria of Helicoverpa armigera. Kanehisa M. India. Copyright © 2012. The KEGG resource for deciphering the genome. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. Sci. ruplal@gmail.M. 68. Rajagopal R.125. and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). Vaissayre M. et al. 123 predicted transposase genes but no gene coding for pyruvate kinase. The genome contains 66 tandem repeats. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra. Okuno Y. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme. All Rights Reserved. 2008. representing all amino acids.b Jitendra P. REFERENCES 1.P. and the coding density was calculated to be 84. Delhi. Indiab In this study. Goto S. and Acinetobacter baumannii ACICU (score.0 (2). American Society for Microbiology. The RAST Server: rapid annotations using subsystems technology. Among these. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. Kawashima S. Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra.

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