Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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5. REFERENCES 1.asm. Kajla MK. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. All Rights Reserved. N. A.085 bp with an average G⫹C content of 41%. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP.911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation. American Society for Microbiology.125. 2012. doi:10.a Naseer Sangwan. The RAST Server: rapid annotations using subsystems technology. A. 2. and the coding density was calculated to be 84. The KEGG resource for deciphering the genome.nlm. The final assembly was validated using paired-end constraints (N50 contigs. Sci. Nucleic Acids Res. p. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme. and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). Nucleotide sequence accession number. we report the draft genome sequence (3. BMC Genomics 9:75. RNAmmer: consistent and rapid annotation of ribosomal RNA genes.S. Hattori M. Government of India. and Acinetobacter baumannii ACICU (score.3 set at a k-mer length of 25 (3).D.1128/JB.555 reads) that led to ⬃45-fold coverage of the entire genome. Copyright © 2012. Four copies of 5S rRNA.. and N..b Jitendra P. Goto S. 4. 65:1147–1154. Host plant induced variation in gut bacteria of Helicoverpa armigera. Aziz RK. Rajagopal R. RAST results also indicated Acinetobacter lwoffii SH145 (score. India (7). Manag. 35:3100 –3108. Nucleic Acids Res. India. Ojha A. HA will further 5156 jb.M.a Ankita Dua. 2 copies of 23S rRNA. Okuno Y. A total of 2.a Udita Mukherjee. Khurana. 6.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology. Ghogomu RT. The draft genome was annotated using RAST version 4.P.M. doi:10. Among these.125.4 kb). Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra. 2. J. Achaleke J.0 (2). In addition. Sixtyfour predicted tRNA genes. ruplal@gmail. acknowledge the Council of Scientific and Industrial Research. Kanehisa M.pone. Raj A.0. J.. et al. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. 2012 by guest A . 511. 518).S. strain HA is available in GenBank under accession number AJXD00000000. and the KEGG database (4). Brévault T. et al. Also.a Neeti Pandey.asm. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin. Here. University Grants Commission. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. 1997. Acinetobacter baumannii AB0057 (score. 518) as the closest neighbors of strain HA. Pest.nih . 2008. Biol. 17:1519 –1533. 2007. Strain HA. and toxic compounds were found. http://www.83%. Lowe TM. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0.a Paramjit Khurana. Boisvert S. 3. University of Delhi. and a GⴙC content of Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. Vaissayre M. Nucleic Acids Res. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. 68. Eddy SR. The genome contains 66 tandem repeats. Comput.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp.0030768.085 bp) of this strain that consists of 102 contigs. 7. the presence of genes coding for esterase enzyme have been observed. New Delhi. U. However. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library.. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra. 2009.html). Lagesen K. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. HA represents a genome size of 3.a Anjali Saxena. 2010.b and Rup Lala Department of Zoology.911 predicted coding sequences. Kawashima S. Martin T. 520). Corbeil J.ncbi.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. cinetobacter sp. were identified by using tRNAscan-SE (6). Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics. a key component in carbohydrate University of Delhi South Campus.1371/journal. Detailed analysis of the genome sequence of Acinetobacter sp. The genome sequence of Acinetobacter sp. Acinetobacter on September 17.. Here. Laviolette F. Indiab In this study. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. were found. PLoS One 7:e30768. The draft genome of Acinetobacter sp. which can thus contribute to insect resistance (1).a Raman Rajagopal. 32:277– 280. for providing research fellowships. Delhi. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. Priya NG. 25: 955–964. representing all amino 123 predicted transposase genes but no gene coding for pyruvate kinase. 2004.

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