Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
armigera
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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4 kb). Kajla MK. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. which can thus contribute to insect resistance (1).01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. A. Among these. et al. 2010. ruplal@gmail. U.0 (2). Acinetobacter sp. Nucleic Acids Res.. Strain HA.org/ on September 17. Okuno Y.911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation. and N. Priya NG.83%. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Rajagopal R. 2012 by guest A . Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra. J. 1997. Nucleic Acids Res. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0. 7. However. Laviolette F.125.b and Rup Lala Department of Zoology.085 bp) of this strain that consists of 102 contigs.. 3. BMC Genomics 9:75. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. Government of India. doi:10.asm. 6. N. Detailed analysis of the genome sequence of Acinetobacter sp.a Paramjit Khurana.1128/JB. doi:10. 68. for providing research fellowships. acknowledge the Council of Scientific and Industrial Research. Four copies of 5S rRNA.a Anjali Saxena.a Udita Mukherjee. The draft genome was annotated using RAST version 4.M. Brévault T. Indiab In this study. Acinetobacter baumannii AB0057 (score.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. Biol.ncbi. Boisvert S. The RAST Server: rapid annotations using subsystems technology. 25: 955–964. 2. Vaissayre M. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme.b Jitendra P.085 bp with an average G⫹C content of 41%. HA will further 5156 jb. Aziz RK.P.a Raman Rajagopal. Kanehisa M. Also. In addition.gov/genomes/static/Pipeline. A total of 2. Here. a key component in carbohydrate metabolism. Delhi. A. 2. 2 copies of 23S rRNA. 2012. 2009.0030768. University of Delhi South Campus. University Grants Commission. Corbeil J. and a GⴙC content of 41%. Host plant induced variation in gut bacteria of Helicoverpa armigera.org Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. 2007.. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.3 set at a k-mer length of 25 (3). All Rights Reserved. The genome contains 66 tandem repeats.S.0. The final assembly was validated using paired-end constraints (N50 contigs. American Society for Microbiology.a Ankita Dua. and the coding density was calculated to be 84. The genome sequence of Acinetobacter sp. 5. 32:277– 280. were found.S. Lagesen K.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology.html).pone. The KEGG resource for deciphering the genome. PLoS One 7:e30768. Nucleic Acids Res. Lowe TM. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. Hattori M. India (7). and toxic compounds were found. strain HA is available in GenBank under accession number AJXD00000000. 518) as the closest neighbors of strain HA. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics.D. Achaleke J. 520). Ghogomu RT.911 predicted coding sequences. India. Comput. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin. Goto S. Copyright © 2012. The draft genome of Acinetobacter sp.1371/journal. Khurana. REFERENCES 1. 2004. Kawashima S. Nucleotide sequence accession number.a Neeti Pandey. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. and the KEGG database (4). University of Delhi. http://www. Pest. 65:1147–1154.com. 35:3100 –3108.125. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.M. Sci. p. 4. cinetobacter sp.. 123 predicted transposase genes but no gene coding for pyruvate kinase. Ojha A. RAST results also indicated Acinetobacter lwoffii SH145 (score. et al. representing all amino acids.a Naseer Sangwan. we report the draft genome sequence (3.asm. Here. New Delhi.555 reads) that led to ⬃45-fold coverage of the entire genome. the presence of genes coding for esterase enzyme have been observed. Martin T. Sixtyfour predicted tRNA genes. and Acinetobacter baumannii ACICU (score. Eddy SR. 17:1519 –1533. HA represents a genome size of 3. were identified by using tRNAscan-SE (6).. 511. Manag. Raj A.nlm. J. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. 2008.nih . 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. 518). and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5).

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