Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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gov/genomes/static/Pipeline.b and Rup Lala Department of Zoology.0030768. However. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. Sci.a Udita Mukherjee. and a GⴙC content of 41%. and toxic compounds were found. RAST results also indicated Acinetobacter lwoffii SH145 (score. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. Acinetobacter baumannii AB0057 (score. 518) as the closest neighbors of strain HA. Boisvert S. 7. University Grants Commission. Lowe TM. PLoS One 7:e30768. ruplal@gmail. et al. Laviolette F. Biol. Kawashima S. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra. Indiab In this study. In addition. All Rights Reserved.a Raman Rajagopal. Manag. Lagesen K. Delhi.3 set at a k-mer length of 25 (3). Raj A. India. A total of 2. Also. 2012. 518).085 bp) of this strain that consists of 102 contigs. and N. Priya NG. REFERENCES 1. et al. Government of India. Achaleke J.125. Goto S. The draft genome of Acinetobacter sp.0. Kajla MK. Nucleic Acids Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. for providing research fellowships. Acinetobacter sp.1128/JB. were found. Here. India (7). Among these. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. Copyright © 2012. The KEGG resource for deciphering the genome. The final assembly was validated using paired-end constraints (N50 contigs. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. Comput. Nucleotide sequence accession number. A. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. N. Sixtyfour predicted tRNA genes. strain HA is available in GenBank under accession number AJXD00000000. HA will further 5156 on September 17. Okuno Y. Here.125. 520). 3.0 (2). 65:1147–1154. Eddy SR.a Neeti Pandey.nih . J. Kanehisa M.83%.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. p. we report the draft genome sequence (3. American Society for Microbiology. U.P. BMC Genomics 9:75. HA represents a genome size of 3. were identified by using tRNAscan-SE (6). 6. 2007.D. The draft genome was annotated using RAST version 4.pone. Nucleic Acids Res. The genome sequence of Acinetobacter sp. 17:1519 –1533. Hattori M. and the coding density was calculated to be 84. 32:277– 280. 2012 by guest A . University of Delhi. Brévault T.S. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. 5.a Naseer Sangwan. 2. The genome contains 66 tandem repeats. 25: 955–964. http://www. doi:10. A.ncbi. 123 predicted transposase genes but no gene coding for pyruvate kinase. and Acinetobacter baumannii ACICU (score. The RAST Server: rapid annotations using subsystems technology. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics.. a key component in carbohydrate metabolism.911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation. 2009. Host plant induced variation in gut bacteria of Helicoverpa armigera. Four copies of 5S rRNA.085 bp with an average G⫹C content of 41%..a Anjali Saxena. Vaissayre M.a Paramjit Khurana. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme.911 predicted coding sequences. doi:10.S.. Pest. Strain HA.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. 2 copies of 23S rRNA. representing all amino acids. Aziz RK. the presence of genes coding for esterase enzyme have been J.asm..4 kb). and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra.asm. University of Delhi South Campus.555 reads) that led to ⬃45-fold coverage of the entire genome.b Jitendra P. 35:3100 –3108. Ghogomu RT. 4. Detailed analysis of the genome sequence of Acinetobacter sp. RNAmmer: consistent and rapid annotation of ribosomal RNA genes.M. Corbeil J. and the KEGG database (4). The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0.nlm. cinetobacter sp. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. Ojha A. 2008. 1997.a Ankita Dua. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. Khurana. Nucleic Acids Res. 68. Martin T. Rajagopal R. 2004.. 2.1371/journal.html). 2010. which can thus contribute to insect resistance (1). acknowledge the Council of Scientific and Industrial Research.M. 511. New Delhi.

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