Genome Sequence of Acinetobacter sp.
Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.
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Nucleic Acids Res. 2012. The
KEGG resource for deciphering the genome.S. Comput.
This work was supported by grants from the DU/DST PURSE Programme. A. The genome contains 66 tandem
Sci. Eddy SR. A total
of 2.a Anjali Saxena. Indiab
In this study. were found. the presence
of genes coding for esterase enzyme have been observed. and
a GⴙC content of 41%.085 bp with an average G⫹C content of 41%. Nucleic Acids Res. 2012 by guest
.html). Ghogomu RT. Nucleic Acids Res. N. 5156
Received 3 July 2012 Accepted 6 July 2012
Address correspondence to Rup Lal.
Nucleotide sequence accession number.P. Ray: simultaneous assembly of
reads from a mix of high-throughput sequencing technologies.gov/genomes/static/Pipeline. 2... http://www. ruplal@gmail. 511. acknowledge the Council
of Scientific and Industrial Research. 9 genes coding for cobalt-zinc-cadmium resistance and
14 coding for multidrug resistance efflux pumps were found. University of Delhi. Kanehisa M. Pest. representing all amino acids.M.
Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.1128/JB.
Government of India.
5. Laviolette F.
The draft genome of Acinetobacter sp. 35:3100 –3108.b Jitendra P. and the coding density was calculated to be 84. Kajla MK. University of Delhi
South Campus. Here. Also.0. Khurana. 65:1147–1154.com. New Delhi.125. a key
component in carbohydrate metabolism. we report the draft genome sequence of strain
HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. Manag. 2004. Achaleke J. doi:10.
p.D. Sixtyfour predicted tRNA genes. University Grants Commission. were
identified by using tRNAscan-SE (6). 518).
6. and N. HA will further
jb. 2007.ncbi.asm. Acinetobacter sp. 2008.a Naseer Sangwan. The RAST Server: rapid annotations using subsystems
technology. Host plant
induced variation in gut bacteria of Helicoverpa armigera. Boisvert S. Aziz RK.
3.a Udita Mukherjee. All Rights Reserved. strain HA was isolated from the midgut of a
fifth-instar larva of Helicoverpa armigera collected from an
agricultural field in Maharashtra. India (7). 123 predicted
transposase genes but no gene coding for pyruvate kinase. 2010..pone.
2. and the KEGG database (4).
7. Raj A. Kawashima S. Four copies of 5S rRNA. et al. U.
Copyright © 2012.83%. and 2 copies of 16S rRNA were found in the
sequence by using RNAmmer (5)..a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology.911 protein-coding sequences and 932 hypothetical proteins
were predicted on annotation.a Neeti Pandey.1371/journal. Annotations by RAST revealed 383 subsystems and 45 genes relevant to
resistance to antibiotics. 1997. Priya NG. 32:277–
280. which can thus contribute to insect resistance (1). In addition. Isolated from the
Gut of the Polyphagous Insect Pest Helicoverpa armigera
cinetobacter sp. A. Okuno Y.nlm.4 kb). Brévault T. and Acinetobacter baumannii ACICU (score. Detailed
analysis of the genome sequence of Acinetobacter sp.3 set at a k-mer
length of 25 (3).M.555 reads) that led to ⬃45-fold coverage of the
entire genome. The strain exhibited
enhanced esterase activity and facilitated the metabolism of the
insecticide cypermethrin.085 bp) of this strain that consists of 102 contigs. Goto S.911 predicted coding sequences. we
report the draft genome sequence (3. Acinetobacter baumannii AB0057 (score.0030768. strain HA is available in GenBank under accession number AJXD00000000. 3
polyglutamic acid (PGA) synthesis-related protein-coding genes
responsible for biofilm formation were present. NCBI Prokaryotic Genomes
Automatic Annotation Pipeline (PGAAP.
Among these.org/ on September 17. et al. Vaissayre M. for providing research fellowships. and toxic compounds were found. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Lagesen K. BMC Genomics 9:75. Lowe TM.01194-12
September 2012 Volume 194 Number 18
Downloaded from http://jb. American Society for Microbiology.0 (2). The final assembly was validated using paired-end
constraints (N50 contigs.125.asm. J. Corbeil J. Hattori M. 2
copies of 23S rRNA. Ojha A. 518) as the closest neighbors of strain HA. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0. J. The genome sequence of Acinetobacter sp. 25:
. Martin T. Here. RAST
results also indicated Acinetobacter lwoffii SH145 (score.a Ankita Dua. The draft genome was annotated using RAST version 4. PLoS One
7:e30768. 17:1519 –1533. 520). HA represents a genome
size of 3.org
Journal of Bacteriology
provide a comprehensive understanding toward predicting its role in
metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. However.a Raman Rajagopal.GENOME ANNOUNCEMENT
Genome Sequence of Acinetobacter sp.a
Paramjit Khurana. Rajagopal R. 2009. Delhi.. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera.
Biol. India. 68. Strain HA.b and Rup Lala
Department of Zoology. RNAmmer: consistent and rapid annotation of
ribosomal RNA genes.