Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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2010. Indiab In this study. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0.S. India. Vaissayre M. 68. and Acinetobacter baumannii ACICU (score..125. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin. Nucleotide sequence accession number. 1997. 35:3100 –3108. Ghogomu RT. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. Delhi.asm. Strain HA. REFERENCES 1. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.085 bp) of this strain that consists of 102 contigs. University of Delhi South Campus. Nucleic Acids Res. and the coding density was calculated to be 84. Four copies of 5S rRNA. HA represents a genome size of 3.html).0 (2). strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra.. Lagesen K. Here. were found. The genome contains 66 tandem repeats.nih . ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme. Kajla MK. et al. and N. However. and the KEGG database (4). Eddy SR. Aziz RK. N. Laviolette F.S. a key component in carbohydrate metabolism. Kawashima S.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology. 4. 511.3 set at a k-mer length of 25 (3). Raj A. 2004. Khurana.125. Priya NG. and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). Rajagopal R. The final assembly was validated using paired-end constraints (N50 contigs. doi:10. 123 predicted transposase genes but no gene coding for pyruvate kinase. we report the draft genome sequence (3.0030768. 2012.a Naseer Sangwan. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. 3. Okuno Y.911 predicted coding sequences. http://www.a Paramjit Khurana.a Ankita Dua. Here. Corbeil J. Host plant induced variation in gut bacteria of Helicoverpa armigera. Lowe TM. 520). 518) as the closest neighbors of strain HA. Copyright © 2012. Goto S. A. Ojha A. India (7). In addition. 2012 by guest A . Biol.a Udita protein-coding sequences and 932 hypothetical proteins were predicted on annotation. The draft genome of Acinetobacter sp. were identified by using tRNAscan-SE (6). the presence of genes coding for esterase enzyme have been observed. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. The KEGG resource for deciphering the genome. cinetobacter sp. University Grants Commission. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra. 2008. 2 copies of 23S rRNA. PLoS One 7:e30768. 17:1519 –1533. and toxic compounds were found. et al.a Anjali Saxena. Acinetobacter baumannii AB0057 ( on September 17.4 kb). RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Hattori Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera.1128/JB. Nucleic Acids Res.pone. 5.085 bp with an average G⫹C content of 41%. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. BMC Genomics 9:75. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. 7. All Rights Reserved.M. Detailed analysis of the genome sequence of Acinetobacter sp. Also. which can thus contribute to insect resistance (1).a Neeti Pandey.b and Rup Lala Department of Zoology. Acinetobacter sp. Kanehisa M.a Raman Rajagopal.1371/journal.nlm.D. representing all amino acids. Martin T. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. A total of 2. A. The genome sequence of Acinetobacter sp. University of Delhi. Nucleic Acids Res. Pest.. 6. Government of India. Boisvert S. Achaleke J.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. Comput..0. 2007. 518). ruplal@gmail. 2. The draft genome was annotated using RAST version 4. Sixtyfour predicted tRNA genes. acknowledge the Council of Scientific and Industrial Research. for providing research fellowships.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. New Delhi. J. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics. J. American Society for Microbiology. 2009. doi:10. strain HA is available in GenBank under accession number AJXD00000000. The RAST Server: rapid annotations using subsystems technology.b Jitendra P. Brévault T.M.83%.. and a GⴙC content of 41%. 32:277– 280.ncbi. RAST results also indicated Acinetobacter lwoffii SH145 (score. 2. HA will further 5156 jb. Manag. 65:1147–1154.P. 25: 955–964. p.555 reads) that led to ⬃45-fold coverage of the entire genome.asm. Among these. U.