Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
armigera
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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A. ruplal@gmail. et al. Nucleic Acids Res. Lagesen K. Lowe TM. India (7). J. a key component in carbohydrate metabolism.nlm. Acinetobacter sp. However. RAST results also indicated Acinetobacter lwoffii SH145 (score. 4. Rajagopal R. acknowledge the Council of Scientific and Industrial Research. and toxic compounds were found. Also. and Acinetobacter baumannii ACICU (score. 2010. Among these. 518). Martin T. for providing research fellowships. the presence of genes coding for esterase enzyme have been observed. PLoS One 7:e30768. A. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0. REFERENCES 1. Indiab In this study. 2008. and the coding density was calculated to be 84. All Rights Reserved.a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics. 520). 25: 955–964.1371/journal. University of Delhi South Campus. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin. 7. Copyright © 2012. Achaleke J. 3. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera.125.1128/JB.a Raman Rajagopal.a Paramjit Khurana. representing all amino acids.M.a Anjali Saxena.911 predicted coding sequences. 2. Detailed analysis of the genome sequence of Acinetobacter sp. 35:3100 –3108. Raj A.0 (2). Ghogomu RT. N..0030768. Aziz RK. cinetobacter sp.b Jitendra P. were found. et al. Sci. Acinetobacter baumannii AB0057 (score. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. J. Host plant induced variation in gut bacteria of Helicoverpa armigera. and N. Khurana. Boisvert S.M.0.com.pone. Ojha A. 68. which can thus contribute to insect resistance (1).html). Strain HA. The final assembly was validated using paired-end constraints (N50 contigs. A total of 2. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. New Delhi. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. Four copies of 5S rRNA. The genome sequence of Acinetobacter sp.P. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra. 1997. Government of India.asm. HA represents a genome size of 3.org Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. 2007.b and Rup Lala Department of Zoology..a Neeti Pandey. Okuno Y. we report the draft genome sequence (3.asm.085 bp with an average G⫹C content of 41%.org/ on September 17. 511. 17:1519 –1533.4 kb). Hattori M. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.a Ankita Dua.S. In addition.125.. University of Delhi. doi:10.3 set at a k-mer length of 25 (3). 2012 by guest A . strain HA is available in GenBank under accession number AJXD00000000. 518) as the closest neighbors of strain HA. 2009. 32:277– 280. Kawashima S. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme. and a GⴙC content of 41%.D. The KEGG resource for deciphering the genome. U. India. Nucleic Acids Res. The genome contains 66 tandem repeats.555 reads) that led to ⬃45-fold coverage of the entire genome. and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). Biol. Priya NG. Delhi. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. doi:10. http://www.S. The draft genome was annotated using RAST version 4. Nucleic Acids Res. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. and the KEGG database (4). Eddy SR. 65:1147–1154.ncbi. were identified by using tRNAscan-SE (6).911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation. Corbeil J. Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra. University Grants Commission. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75.a Udita Mukherjee.. Kajla MK. 2004. Kanehisa M. 2012. American Society for Microbiology. Nucleotide sequence accession number. 5. HA will further 5156 jb. Manag.gov/genomes/static/Pipeline. Vaissayre M. Comput.83%. Here.. Pest.nih . 6. Here. Laviolette F. p. 123 predicted transposase genes but no gene coding for pyruvate kinase. Goto S.085 bp) of this strain that consists of 102 contigs. 2 copies of 23S rRNA. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. Sixtyfour predicted tRNA genes.a Naseer Sangwan. 2. The draft genome of Acinetobacter sp.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. Brévault T.

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