Genome Sequence of Acinetobacter sp.

Strain HA, Isolated from the Gut of the
Polyphagous Insect Pest Helicoverpa
Jaya Malhotra, Ankita Dua, Anjali Saxena, Naseer Sangwan,
Udita Mukherjee, Neeti Pandey, Raman Rajagopal, Paramjit
Khurana, Jitendra P. Khurana and Rup Lal
J. Bacteriol. 2012, 194(18):5156. DOI: 10.1128/JB.01194-12.

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Lowe TM. we report the draft genome sequence of strain HA using the Roche 454 GS (FLX Titanium) system (3-kb pairedend library. Nucleic Acids Res. HA will further 5156 jb.GENOME ANNOUNCEMENT Genome Sequence of Acinetobacter sp. 2012. The draft genome of Acinetobacter sp. Vaissayre M.pone. we report the draft genome sequence (3. 2 copies of 23S rRNA. The genome contains 66 tandem repeats. Pest. Boisvert S. 35:3100 –3108. Host plant induced variation in gut bacteria of Helicoverpa armigera.a Naseer Sangwan. Achaleke J. Aziz RK.D.nlm. University of Delhi. 2012 by guest A .com. 2009.asm.a Ankita Dua. HA represents a genome size of 3. The reads generated were assembled into 102 contigs by using Ray de novo assembler version 0. 25: 955–964. Brévault T. Biol.0 (2).html). Lagesen K. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera collected from an agricultural field in Maharashtra.b Jitendra P. Manag.085 bp) of this strain that consists of 102 contigs. which can thus contribute to insect resistance (1).org Journal of Bacteriology provide a comprehensive understanding toward predicting its role in metabolizing insecticides and hence conferring resistance to Helicoverpa armigera. Eddy SR. A. 2008. 511. Here. 17:1519 –1533. BMC Genomics 9:75. Annotations by RAST revealed 383 subsystems and 45 genes relevant to resistance to antibiotics. Also.0. NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP. 5156 Received 3 July 2012 Accepted 6 July 2012 Address correspondence to Rup Lal. Here.1128/JB. India (7). The RAST Server: rapid annotations using subsystems technology. Government of India. New Delhi. Hattori M. 7. 2004. 6. The draft genome was annotated using RAST version 4. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Kawashima S. REFERENCES 1. Khurana. Acinetobacter baumannii AB0057 (score.3 set at a k-mer length of 25 (3). and the coding density was calculated to be 84. 2. Ray: simultaneous assembly of reads from a mix of high-throughput sequencing technologies. acknowledge the Council of Scientific and Industrial Research. Sci. Copyright © 2012. Sixtyfour predicted tRNA genes. and toxic compounds were found. University of Delhi South Campus. Indiab In this study.a Udita Mukherjee. Ojha A. 518).555 reads) that led to ⬃45-fold coverage of the entire genome.a Paramjit Khurana. Kanehisa M.4 kb). Rajagopal R. were found. Among these. et al. Raj A.b and Rup Lala Department of Zoology.ncbi. Isolated from the Gut of the Polyphagous Insect Pest Helicoverpa armigera Jaya Malhotra.. and Acinetobacter baumannii ACICU (score. U. Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. the presence of genes coding for esterase enzyme have been observed.M.01194-12 September 2012 Volume 194 Number 18 Downloaded from http://jb. Nucleic Acids Res. However. ruplal@gmail. 2007. doi:10.0030768. Acinetobacter sp. RAST results also indicated Acinetobacter lwoffii SH145 (score.a Neeti Pandey. J. 4.S. J. 5. p. PLoS One 7:e30768. and N. 123 predicted transposase genes but no gene coding for pyruvate kinase. 65:1147–1154. Ghogomu RT.. 3 polyglutamic acid (PGA) synthesis-related protein-coding genes responsible for biofilm formation were present. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.asm. 2010. 3.a Anjali Saxena.125. 9 genes coding for cobalt-zinc-cadmium resistance and 14 coding for multidrug resistance efflux pumps were found. University Grants Commission. representing all amino acids.nih .a and Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology. http://www. 32:277– 280. Four copies of 5S rRNA. Comput. Martin T. Nucleic Acids Res. Corbeil J.. In addition.P. ACKNOWLEDGMENTS This work was supported by grants from the DU/DST PURSE Programme.125. Okuno Y.911 protein-coding sequences and 932 hypothetical proteins were predicted on annotation. The genome sequence of Acinetobacter sp. All Rights Reserved. for providing research fellowships.085 bp with an average G⫹C content of 41%. The strain exhibited enhanced esterase activity and facilitated the metabolism of the insecticide cypermethrin. strain HA was isolated from the midgut of a fifth-instar larva of Helicoverpa armigera. et al. doi:10. The KEGG resource for deciphering the genome. American Society for Microbiology.. Priya NG. 1997.1371/journal. Kajla MK. A total of Laviolette F. N. strain HA is available in GenBank under accession number AJXD00000000. 520). cinetobacter sp. a key component in carbohydrate metabolism.911 predicted coding sequences. 518) as the closest neighbors of strain HA.. and the KEGG database (4). and 2 copies of 16S rRNA were found in the sequence by using RNAmmer (5). 2. 68. Strain HA.M. Nucleotide sequence accession number. The final assembly was validated using paired-end constraints (N50 contigs.S. India. Goto S. were identified by using tRNAscan-SE (6). Delhi. and a GⴙC content of 41%. on September 17. Detailed analysis of the genome sequence of Acinetobacter sp.83%.a Raman Rajagopal.