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1 April 2003
Product note from the Spectroscopy Products Division
Raman scattering
Raman spectroscopy involves illuminating
a sample with intense monochromatic
light, and analysing the resulting Raman
scattered light. The Raman scattered light
carries information about the identity of
the material, and its chemical and
physical state.
Excitation sources
Lasers are the ideal excitation source for
Raman spectroscopy as they emit intense,
highly-collimated, monochromatic light.
inVia Raman microscopes can employ a wide
range of lasers, offering excitation
wavelengths ranging from the near-IR to the
deep ultraviolet (see Table 1, on back page).
The most appropriate excitation wavelength
depends on the application involved.
If the sample contains a strong chromophore
(for example a paint pigment or dye), near-IR
or UV excitation may be preferable to visible
excitation as the latters Raman signal may
be obscured by strong fluorescence (Figs. 1
and 2).
visible excitation
Multiple lasers
Laboratories studying a wide range of
sample types often wish to employ Raman
systems with varied excitation
wavelengths from several lasers.
Fluorescence
dominates
Raman
dominates
514 nm
(Ar+)
1500
2000
Figure 1
Spectra from dentine (calcified tissue from a
tooth). The near-IR spectrum has greatly
reduced fluorescence and shows discernible
Raman features not seen in the visible
spectrum.
2500
near-infrared excitation
1000
It is important to choose
the best excitation
wavelengths for your
application when
configuring your inVia
Raman microscope.
Fluorescence
dominates
500
633 nm
(HeNe)
18000
16000
785 nm
(diode)
14000
12000
Figure 2
Spectra from a conducting polymer. The 785 nm
excitation spectrum clearly shows Raman
bands, whereas the visible excitation spectra
(514 nm and 633 nm) are dominated by strong
fluorescence.
(Vertical axes scaled for clarity)
Easy to use
The fully automated inVia
Reflex enables you to
switch excitation
wavelengths under
software control, with the
minimum of fuss.
Rayleigh scattering
The vast majority of the light scattered
from the sample is elastically scattered
(Rayleigh scattered) and carries very
little useful information.
Filter technologies
x0.2
(offset for clarity)
transmission
five monolayer
notch filter
edge filter
C16H33O
OH
one monolayer
1200
1400
1600
1800
Figure 3
UV resonance Raman spectra of molecular
monolayers.
Data courtesy of Dr. Zhou Bing, Institute of
Theoretical Chemistry, Jilin University,
Peoples Republic of China.
0
Raman shift (cm-1)
Figure 4
Schematic diagram of notch and edge filters.
Easy to upgrade
25
50
75
100
125
Raman shift / cm-1
150
175
10
15
20
25
30
35
Figure 5
Spectra of cadmium (II) iodide (CdI2) taken
using holographic notch filters and with a NExT
filter. Note the two low wavenumber bands that
are only visible in the NExT spectrum.
Figure 6
NExT filter spectrum of an InAs/GaAs
superlattice showing the folded longitudinal
acoustic modes.
40
Renishaw plc
Spectroscopy Products Division
Old Town, Wotton-under-Edge,
Gloucestershire GL12 7DW
United Kingdom
Common excitation
wavelengthsa
Laser type
diode
diode
Visible spectroscopy
and 2-D imaging
gas
YAGb
gas
gas
gas
UV spectroscopy
Notes
a
b
633
532
514
488
442
nm
nm
nm
nm
nm
325 nm
244 nm
gas
gasb
laser
excitation
wavelengths
325 nm
244 nm
wavenumber / cm-1
40k
energy / eV
30k
frequency / THz
830nm
785 nm
633 nm
532 nm
514 nm
488 nm
442 nm
1200
20k
4
1000
3
800
10k
2
600
1
400
200
Suitability
Filter type
Low limitc
Near-IR excitation
holographicd
dielectric
NExT filtere
100 cm-1
200 cm-1
10 cm-1
Visible excitation
holographic (special)
holographic (standard)
dielectric
NExT filter
50 cm-1
100 cm-1
200 cm-1
12 cm-1
UV excitation
(325 nm)
dielectric
NExT filter
UV excitation
(244 nm)
dielectric
NExT filter
Notes
c
d
e
holographicd
dielectric
NExTe
near-IR
excitation
visible
excitation
holographic (special)
holographic (standard)
dielectric
NExT
350 cm-1
40 cm-1
UV excitation
325 nm
dielectric
NExT
500 cm-1
100 cm-1
UV excitation
244 nm
dielectric
NExT
Raman shift / cm-1 0
100
200
300
400
500
Acknowledgements
Laser safety
A class 1 enclosure is
available as an option.