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ISSN: 2229-3701
___________________________________________________________Research Paper
3Dhanvanthari
___________________________________________________________________________
ABSTRACT
The present study aimed at evaluating the in vitro antimicrobial effect of crude extract of locally available
plants, Musa paradisiaca and Cocos nucifera on bacteria (Escherichia coli, Staphylococcus aureus, Bacillus
subtilis, Pseudomonas aeruginosa) and fungi (Candida albicans, Candida tropicalis, Aspergillus niger). The agar
disc diffusion method was used to determine the inhibitory effect of both the test plants. Both the plants
extract showed inhibitory effect on test organisms. The extract of Musa paradisiaca produced wider zones of
inhibition against Candida spp. than the crude extract of Cocos nucifera. The minimum inhibitory
concentration was also evaluated for the extracts
Key Words: Antimicrobial activity, Musa paradisiaca, Cocos nucifera, Minimum inhibitory concentration
INTRODUCTION
Even though pharmacological industries have
produced a number of new antibiotics in the last
three decades, resistance to these drugs by
microorganisms has increased. The problem of
microbial resistance is growing and the outlook for
the use of antimicrobial drugs in the future is still
uncertain. Therefore, actions must be taken to
reduce this problem, for example, to control the use
of antibiotic, develop research to better understand
the genetic mechanisms of resistance, and to
continue studies to develop new drugs, either
synthetic or natural. The ultimate goal is to offer
appropriate and efficient antimicrobial drugs to the
patient.
For a long period of time, plants have been a
valuable source of natural products for maintaining
human health, especially in the last decade, with
more intensive studies for natural therapies. About
80% of individuals from developed countries use
traditional medicine, which has compounds derived
from medicinal plants. Therefore, such plants
should be investigated to better understand their
properties, safety and efficiency1. The use of plant
extracts and phytochemicals, both with known
antimicrobial properties, can be of great
________________________________________
*Address for correspondence:
E-mail: arpan84shah@yahoo.co.in
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264
Extraction procedure
Grounded plant materials (40 gm) were
extracted
in
soxhlet
apparatus
with
dichloromethane and methanol (1:1) at 70oC till
exhaustion. Solvent was recovered; extracts were
concentrated and allowed to dry at room
temperature.
Test Organisms
Cultures of the microorganism, used in these
studies were obtained from Department of
Biotechnology, K.L.E.Ss College of Pharmacy
are: Escherichia coli, Staphylococcus aureus,
Bacillus subtilis, Pseudomonas aeruginosa,
Candida albicans, Candida tropicalis, and
Aspergillus niger. Stock cultures of Bacteria and
fungi were maintained on Nutrient agar and Potato
Dextrose Agar slants, respectively. All cultures
were subcultured monthly and subsequently stored
at 40C.
Antimicrobial activity
It was performed by agar diffusion method
using a paper disc. Nutrient agar (purchased from
Hi- Media) and sabourauds dextrose agar
(purchased from Hi- Media) was used for bacterial
and fungal strains respectively. The sterilized
(autoclaved at 1210C for 20 mins) medium (40500C) was inoculated (1ml/100ml of medium) with
the suspension of microorganism. The paper discs
impregnated with extracts (10, 30, 50 and 100
g/ml in dimethyl sulphoxide) was placed on the
solidified medium. The plates were preincubated
for 1 hr at room temperature and then incubated at
370C for 24 and 300C at 48 hrs for antibacterial and
antifungal activities respectively. Cephalexin (30
g/disc, purchased from Hi-media and Fluconazole
(10 g/disc, marketed product of cipla was used
i.e.Fluka tablet) was used as standard for
antibacterial and antifungal activity respectively.
Minimum inhibitory concentration
The MIC was determined by the micro dilution
method using liquid nutrient media with different
aliquots of the test materials. 10 ml of sterilized
double strength nutrient media was poured into
sterilized test tube. From the stock solution (100
g/ ml in DMSO) different concentrations of
extracts were added to double strength nutrient
media, to all tubes and 0.1 ml of bacterial
suspension was added and tube were incubated at
required temperature. The growth was observed for
turbidity and inhibition was determined by absence
of growth. MIC was determined by the lowest
concentration of sample that inhibits the
development of turbidity.
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265
ISSN: 2229-3701
Family
Local name
Areas of collection
Fruit Peel
Belgaum
Musa paradisiaca
Musaceae
Balle hannu
Cocus nucifera
Arecaceae
Thenginakai
Roots
Sambra (Belgaum)
Musa
paradisia
ca
Cocos
nucifera
Std
Conc.
(g/ml)
Fungal strain
B. subtilis
S. aureus
E. coli
P. aeruginosa
C. albicans
C .tropicalis
A. niger
10
12.2 0.1
11 0.1
9.5 0.2
13 0.4
12.5 0.1
10.4 0.1
30
16 0.5
12 0.3
10.8 0.4
11.2 0.2
15.7 0.2
16 0.5
13.2 0.5
50
17
15.4 0.2
13
14.1 0.3
17.2 0.3
17.8 0.6
15.7 0.3
100
21 0.1
18
17.9 0.1
18
24 0.3
23
22.3 0.1
10
8.5 0.7
9 0.3
10 0.2
8.5 0.6
7.4 0.1
30
11.1 0.3
10
13 0.3
10
10.2 0.3
9.1 0.3
50
15.9 0.2
14.1 0.1
14.1 0.3
11.4 0.4
13 0.7
11.9 0.1
12.4 0.2
100
20. 0.1
19.3 0.4
16.3 0.5
15.7 0.1
16.8 0.2
15
17 0.6
25
21
17 0.1
17.6 0.4
23
21
22 0.5
Cephalexin and Fluconazole was used as standard for antibacterial and antifungal activity respectively.
Each zone of inhibition is an average of three independent determination and solvent (DMSO) did not show any inhibition.
Cocos nucifera
B. subtilis
55
75
S. aureus
65
80
E. coli
85
60
P. aeruginosa
75
65
C. albicans
50
65
C .tropicalis
50
70
A. niger
55
60
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266
REFERENCES
1. Ellof JN. Which extractant should be
used for the screening and isolation of
antimicrobial components from plants. J
Ethnopharmacol. 1998;60:1-6.
2. Kubo L, Muroi H and Himejima M,
Structure-antibacterial
activity
relationships of anacardic acids. J Agri
Food Chem. 1993;41:1016-1019.
3. Shapoval EES, Silveira SM, Miranda
ML, Alice CB and Henriques AT.
Evaluation of some pharmacological
activities of Eugenia uniflora. J
Ethnopharmacol. 1994;44:136-142.
4. Jansen AM, Cheffer JJC and Svendsen
AB. Antimicrobial activity of essencial
oils: a 1976-1986 literature review.
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