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Original Article
Correspondence:
Mohammad Ali Takhshid PhD,
Diagnostic Laboratory Sciences and
Technology Research Center,
School of Paramedical Sciences,
Meshkinfam street,
P.O.Box: 71455,
Shiraz, Iran.
Tel/Fax: +98 711 2289113
Email: takhshid2001@yahoo.co.uk
Received: 13 October 2011
Revised: 17 November 2011
Accepted: 4 December 2011
Iran J Med Sci September 2012; Vol 37 No 3
Abstract
Introduction
Endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,4,3-benza-dioxathiepin-3-oxide) is a polycyclic chlorinated
hydrocarbon insecticide. It has been classified as a moderately
hazardous (class II) pesticide. However, it still continues to be
used in agriculture and public health.1 Endosulfan toxicity has been
demonstrated in various organs such as the brain,2 kidney,3 liver,4
heart,5 and reproductive system.
Reproductive toxicity of endosulfan has been shown in some
studies. Endosulfan reduces circulating follicle stimulating hormone
(FSH) and luteinising hormone (LH).6 It has also been associated with
173
Table 1: The values (meanSEM, n=10 each) of body and testes weights of control rats, and rats treated with endosulfan,
endosulfan+vitamin C, endosulfan+vitamin E, or endosulfan+vitamin C and vitamin E
Initial body weight (g) Final body weight (g) Testis weight. (g) Testis wt / Body weight (%)
Control
2649
2738
1.50.1
0.510.01
Endo
2677
27612
1.50.1
0.540.02
Endo+vit C
26310
2729
1.40.2
0.48.02
Endo+vit E
28311
29511
1.40.1
0.53.02
Endo+vit C +vit E
2989
29812
1.40.1
0.49.01
Values represent meanSEM; n=10
Table 2: The values (meanSEM, n=10 each) of sperm parameters of control rats, and rats treated with endosulfan,
endosulfan+vitamin C, endosulfan+vitamin E, or endosulfan+vitamin C and vitamin E
AB+sperm (%)
Treatment
Viability (%)
Sperm motility (%)
DSP (No.106/gr testis)
Control
83.01.5
71.91.9
14.81.2
11.74.4
Endo
26.83.0*
18.01.45*
7.50.8*
42.47.9*
78.12.1
11.40.9*
27.69.2*
Endo+vit C
77.81.5
66.42.7
12.90.5
12.03.4
Endo+vit E
75.42.2
Endo+vit C & vit E
86.21.1
64.32.5
18.31.6
14.45.6
Endo: endosulfan; vit C: vitamin C; vit E: vitamin E; DSP: daily sperm production (DSP); AB+: Aniline Blue positive; *Indicate
significant difference from the control group; Indicate significant difference from treated with Endo alone
175
Figure 1: Concentrations (meanSEM, n=10) of Malondialdehyde (MDA) (A), serum testosterone (B), serum lactate dehydrogenase
(LDH) levels (C) in the control rats and rats treated with endosulfan (Endo), endosulfan+vitamin C (vit C), edosulfan+vitamin E (vit
E) or endosulfan+vitamin C and vitamin E. *indicate significant difference from the control group; #indicate significant difference
from the group receiving endosulfan alone
176
10
11
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