2/4/2015

LabBench

LabQuizII
1.Whichgraphwouldbethemostlikelyresultofperformingthephotosynthesisexperiment
usingfreshchloroplastsplacedinlightandDPIP?

PlantPigmentsand
Photosynthesis
Introduction
4IChromatography
4IIPhotosynthesis
KeyConceptsII

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Concept1:Using
DPIPasanElectron
Acceptor
Concept2:The
Spectrophometer

Designofthe
ExperimentII
AnalysisofResults
II
LabQuizII

a. a
b. b
c. c
d. d
Correct!Answerciscorrectbecauseitshowsanincreaseinthe%transmittance,whichwould
occurasDPIPgoesfrombluetocolorless.WhilegraphAalsoshowsanincreasein
transmittance,itoccursveryslowlyandthereislittletotalincrease.GraphAismoretypicalof
theresultsyoumightgetfromthetubekeptinthedark.
2.WhatisthebestexplanationforgraphB?

a. TheDPIPwastoopaleatthebeginningoftheexperiment.
b. Thechloroplastsolutionwastooconcentrated.
c. Theexperimenterusedchloroplaststhatweredamagedandcouldnotrespondto
light.
d. Theblankwasnotproperlyusedtocalibratethespectrophotometer.
Correct!AnswerciscorrectbecausethiscurveisflatandshowsthatnoDPIPisbeingreduced.
Thismustmeanthechloroplastsarenotfunctioning.
3.WhateffectwouldaddingmoreDPIPtoeachexperimentaltubehaveontheseresults?

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LabBench

a. Eachcurvewouldbeshifteddownwardbutwouldkeepthesamegeneralshape.
b. ThecurveingraphCwouldrisemoresteeplyandleveloffsooner.
c. ThecurveingraphAwouldhavethesamegeneralshapeasthecurveingraphC.
d. Thechloroplastswouldabsorbmorelightenergy,sotherewouldbenochange.
Correct!Answeraiscorrectthesolutionwouldbeadeeperblueinitially,sotransmittance
wouldbeless.
4.WhatistheroleofDPIPinthisexperiment?

a. Itmimicstheactionofchlorophyllbyabsorbinglightenergy.
b. ItservesasanelectrondonorandblockstheformationofNADPH.
c. Itisanelectronacceptorandisreducedbyelectronsfromchlorophyll.
d. Itisbleachedinthepresenceoflight,andcanbeusedtomeasurelightlevels.
Correct!AnswerciscorrectDPIPisreducedinthisexperimentasitreceiveshighenergy
electronsfromchlorophyll.
5.Somestudentswerenotabletogetmanydatapointsinthisexperimentbecausethe
solutionwentfrombluetocolorlessinonly5minutesfortheunboiledchloroplastsexposedto
light.Whatmodificationtotheexperimentdoyouthinkwouldbemostlikelytoprovidebetter
results?

a. Increasethenumberofdropsofchloroplastsusedfrom3to5.
b. DoublethevolumeofDPIPsothatthesolutionhasalowerinitialtransmittance.
c. Modifytheblanksothattheinitialtransmittanceishigher.
d. Usefresherspinachandpreparethechloroplastsolutionduringthelaboratory
procedure.
e. Changethewavelengthatwhichreadingsaretaken.
Correct!AnswerbiscorrectwhenDPIPbecomescolorlessveryrapidlyeithertherearetoo
manycholorplastsortheconcentrationofDPIPissodilutethatthequantitycanbereduced
rapidly.

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