Académique Documents
Professionnel Documents
Culture Documents
QUESTION
NUMBERS
RECALL
COMPRE
HENSION
APPLICA
TION
Bioenergetic, Biological
Oxidation, TCA Cycle, ETC
1 10
1, 5, 8, 9, 10
2, 3, 4, 6, 7
11 30
31 45
46 60
48
Chemistry of Nucleotides
Metabolism of Nucleotides
Chemistry of Nucleic Acid
Molecular Genetics
61 78
70
79 90
83
Other Topics
91 100
99
8. Which of the following enzymes protects aerobic organism against oxygen toxicity?
A. Catalase
C. Homogentisate dioxygenase
B. Cytochrome P 450
D. Superoxide dismutase
(Answer: D / Reference A: p. 90 / MPL 90)
9. The energy from oxidation of components of respiratory chain is coupled to:
A. translocation of protons
C. flow of electrons from NAD
B. chemical potential difference
D. electrical potential difference
(Answer: A / Reference A: p. 95 / MPL 90)
10. Carbon monoxide and cyanide poisoning affect the respiratory chain and specifically both
inhibit:
A. NADH dehydrogenase
C. cytochrome oxidase
B. ubiquinone oxidoreducatase
D. ferricytochrome c oxidoreductase
(Answer: C / Reference A: p. 95 / MPL 90)
11. This substituent attached to @ carbon of amino acids distinguishes amino acids from
each other:
A. Amino group
C. Hydrogen atom
B. Carboxylic group
D. R group
(Answer: D / Reference A: p. 18 / MPL 90)
12. Which of the following amino acyl residues are likely to be found in the interior of a
globular protein?
A. Aspartyl, histidyl, and tyrosinyl residues
B. Alanyl, isoleucyl, and methionly residues
C. Glutamyl, arginyl and asparaginyl residues
D. Cysteinyl, threonyl, and lysyl residues
(Answer: B / Reference A: p. 15-16 / MPL 60)
13. Which of the following interactions is possible between distant amino acyl residues in a
protein?
A. Peptide bond formation between alanine and glutamic residues
B. Disulfice bond formation between two cysteine residues
C. Hydrophobic interaction between asparagine and histidine residues
D. Ionic interaction between tyrosine and glycine residues
(Answer: B / Reference A: p. 15-16, 19, 35. / MPL 60)
14. During protein folding when regions of amino acyl residues have similar psi and phi bond
angles, the protein is said to assume this structural conformation:
A. Primary structure
C. Tertiary structure
B. Secondary structure
D. Quaternary structure
(Answer: B / Reference A: p. 31 / MPL 90)
15. The highest protein structural organization assumed by both functional hemoglobin and Ig
molecules:
A. Primary structure
C. Tertiary structure
B. Secondary structure
D. Quaternary structure
(Answer: D / Reference: p. 40, 591 / MPL 60)
16. Which of the following is CORRECT as regards amino acid and/or protein separation?
A. In electrophoresis of amino acid mixture, both valine and isoleucine move toward the
cathode end.
B. In paper chromatography involving tryptophan, leucine, lysine and threonine, the
amino acid that travels the farthest distance is lysine.
C. In isoelectric focusing of proteins, the protein becomes stationary at pH = its pI and it
has no net charge.
D. The principle of SDS-PAGE in protein subunits determination is based on number of
charge groups in the subunit.
(Answer: C / Reference A: p. 24; B: p 33-35 / MPL 33)
17. Which of the following proteins involve in protein folding or organization causes diseases in
man and animal?
A. Molecular chaperones facilitate appropriate interactions between complementary
groups.
B. Prolyl cis-trans isomerase catalyzes isomerization between cis and trans configuration
of X-Pro peptide bonds.
C. Protein disulfide isomerase facilitates `shuffling of disulfide bonds.
D. PrP facilitates the interconversion of @ helical structure with B pleated sheet.
(Answer: D / Reference A: p. 36-37 / MPL 60)
18. The common biochemical pathology seen in Creutzfeldt-Jakob and Alzheimers
Diseases?
A. Improper folding of affected proteins secondary to absence of chaperonins.
B. The secondary helical structure of affected proteins are transformed into B sheet.
C. The so-called infective agent is the mutated apolipoprotein E.
D. Aggregation of infected proteins forming neurofibrillary tangles.
(Answer: B / Reference A: p. 37 / MPL 60)
19. Synthesis of nutritionally nonessential amino acid involves the:
A. formation of aspartate from a glycolytic intermediate
B. hydroxylation of phenylalanine to form tyrosine
C. interconversion of proline and glutamate via tetrahydrofolate.
D. transamination of @ketoisovalerate to valine
(Answer: B / Reference A: p. 237, 239, 328 / MPL 60)
20. Degradation of which of the following proteins requires ATP and ubiquitin?
A. Elastin
C. Membrane asialoglycoprotein
B. Adenylosuccinase
D. PRPP amidotransferase
(Answer: D / Reference A: p. 242 / MPL 33)
21. The chief mechanism for the removal @ amino group from amino acids is:
A. hydrolytic deamination
C. transamination
B. reductive deamination
D. transamidination
(Answer: C / Reference A: p. 243 / MPL 60)
22. In the urea cycle:
A. the carbons of aspartate are partially incorporated into arginine
B. N-acetylglutamate serves as the allosteric activator of carbamoyl phosphate
synthetase I
C. arginine is the immediate precursor of citrulline
D. energy in the form of 3 moles of GTP is required.
(Answer: B / Reference A: p. 246 / MPL 60)
23. The major mechanism of detoxification of ammonia in the brain tissue is the formation of:
A. @ ketoglutarate
C. succinate
B. glutamine
D. urea
(Answer: B / Reference A: p. 244-245 / MPL 60)
24. Oxidative decarboxylation of which of the following amino acids via a mitochondrial
multienzyme complex that is analogous to pyruvate dehydrogenase complex?
A. histidine, tryptophan, phenylalanine
B. valine, leucine, isoleucine
C. threonine, serine, tyrosine
D. alanine, glycine, asparagine
(Answer: B / Reference A: p. 259 / MPL 60)
25. In amino acid catabolism, which of the following is a CORRECT PAIR as regards clinical
disorder and its associated deficient enzyme?
A. Absence of @ branched chain keto acid decarboxylase Richner Hanhart Syndrome
B. Ornithine transcarbamoylase deficiency Hyperammonemia type 1
C. Hydroxyphenylpyruvate hydroxylase deficiency Classic phenylketonuria
D. Lack of homogentisate oxidase Alkaptonuria
(Answer: D / Reference A: p. 247, 255, 259 / MPL 60)
26. Deficiency of B complex vitamins can lead to decreased enzymatic activity because
derivative of B complex:
A. are allosteric effectors for some enzymes.
B. are important component of active sites.
C. act as co-enzymes of some enzymes.
D. are needed to form ribozymes.
(Answer: C / Reference A: p. 50 / MPL 60)
27. Multiple factors affect the rates of enzyme-catalyzed reactions. The effect of pH is to:
A. increase the kinetic energy of the reacting molecules.
B. mediate the formation of bridges complexes between enzyme and substrate.
C. alter the charged state of the enzyme and substrate.
D. enhance local concentration
(Answer: C / Reference A: p. 64 / MPL 60)
28. Enzymes as biological catalysts speed up chemical reactions because they:
A. alter the delta G0 of the overall reaction.
B. allow substrate binding and catalysis to occur at the active site.
C. lower the activation energy for the formation of transition state.
D. enhance the effect of positive modulators.
(Answer: C / Reference A: p. 61 / MPL 60)
29. Which of the following CORRECTLY characterizes an Ig molecule?
A. Maternal Ig A can readily cross the placenta to give protection to the fetus.
B. Specific Ag binding sites are found in the hypervariable regions of Ig M.
C. Ig G plays an important role in hypersensitivity reactions to allergens.
D. Ig E can activate both the classical and alternate complement systems for
bacteriolysis.
(Answer: B / Reference: p. 591, 594 / MPL 60)
30. Collagen fibers are stabilized by formation of intra and interchain cross links via:
A. S-S bonds and ionic interactions
B. peptide bonds and salt bridges
C. desmosines
D. Schiff bases and aldol condenstion products
C. Polyol pathway
D. Uronic acid pathway
51. The ATP formation in brown adipose tissue is mainly due to:
A. substrate level phosphorylation
C. creatine kinase reaction
B. oxidative phosphorylation
D. adenylyl kinase reaction
(Answer: A / Reference A: p. 217 / MPL 90)
52. B oxidation is inhibited in the fed state because malonyl CoA which arises from acetyl CoA
inhibits:
A. Acetyl CoA carboxylase
C. Carnitine palmitoyl transferase I
B. Acyl CoA dehydrogenase
D. Thiolase
(Answer: C / Reference A: p. 187 / MPL 60)
53. Several drugs block the formation of endogenous cholesterol at various stages in its
biosynthetic pathway. Which of the following acts by inhibiting HMG CoA reductase?
A. choleystyramine resins
C. Sitosterol
B. lovastatin
D. gemfibrozil
(Answer: B / Reference A: p. 229 / MPL 90)
54. TRUE of the regulation of cholesterol synthesis:
A. Transcription of HMG CoA reductase is repressed by cholesterol
B. Insulin inhibits the activity of HMG CoA reductase
C. Dietary cholesterol inhibits the synthesis of cholesterol in most cells.
D. Mevalonate kinase is regulated via covalent modification.
(Answer: A / Reference: p. 220 / MPL 60)
63. Which of the following is responsible for the concerted regulation between purine and
pyrimidine nucleotide de novo pathways?
A. The concerted catalytic activities of the multifunctional catalysts present in both
pathways.
B. The ability of the products in both pathway to inhibit the formation of PRPP.
C. The involvement of common amino acid precursors and carbon dioxide in both
pathways.
D. The allosteric enzyme control mechanisms in both pathways.
(Answer B: A / Reference A: p. 299 / MPL 60)
64. One of the following RNA functions as an enzyme:
A. Small cytoplasmic tRNA catalyzes the attachement of amino acid to its cognate
tRNA.
B. 5.8 s RNA is a peptidyl transferase and catalyzes the formation of peptide bond
during elongation step of translation
C. Small nuclear RNA has catalytic splicing activity and converts primary transcript to
its mature form
D. Only B and C are correct
(Answer: C / Reference A: p. 311 / MPL 60)
65. Common characteristic between B DNA and mRNA:
A. Doublestrandedness with helical grooves
B. The nucleotide units are connected via phosphodiester linkages.
C. 3 and 5 ends of the strand are highly altered and modified.
D. Both have adenine, guanine, cytosine and thymine bases.
(Answer: B / Reference A: p. 303, 309 / MPL 60)
66. Which of the following DNA sequences is most important for translation?
A. Highly repetitive sequence
C. Microsatellite repeat sequence
B. Moderately repetitive sequence
D. Unique sequence
(Answer: D / Reference A: p. 320 / MPL 60)
67. The three important properties of DNA polymerases are:
A. unwinding, chain initiation and elongation
B. chain initiation, elongation and processivity
C. elongation, processivity and proofreading
D. processivity, proofreading and ligation
(Answer: C / Reference A: p. 328 / MPL 90)
68. A common characteristic of replication and transcription:
A. Enzyme involved has proof reading activity.
B. Both strands serve as template simultaneously.
C. Formation of Okazaki fragments or elements
D. Adherence to the Watson-Crick base pairing rules
(Answer: D / Reference A: p. 328, 341 / MPL 60)
69. TRUE of transcription and translation, but not of replication:
A. Both are nuclear events
B. Both need initiator protein factors.
C. Both require DNA as template
D. Both undergo post-synthetic modifications.
(Answer: D / Reference A: p. 326, 341, 364, 365, 371 / MPL 60)
85. Inadequate intake of which vitamins/minerals in the maternal diet may result in
spina bifida in the newborn:
A. Calcium
C. Folic acid
B. Iron
D. Vitamin K
(Answer: C / Reference: p. 494 / MPL 90)
86. One of the following is CORRECT of ascorbic acid:
A. It is a 6-carbon compound closely related to galactose
B. It is a reducing agent and oxygen radical quencher.
C. Megadosing of vitamin C has curative effect against common colds.
D. Its deficiency state is attributed to deficient elastin formation..
(Answer: B / Reference A: p. 495, 496 / MPL 60)
87. Individual suffereing from carcinoid syndrome will also develop pellagra because of:
A. overproduction of 5-hydroxytryptamine
B. defective intestinal transport mechanism for tryptophan
C. lack of enzyme involved in formation of niacin from tryptophan
D. end organ resistance to niacin cellular uptake.
(Answer: A / Reference A: p. 490 / MPL 60)
88. Inhibitors of folate metabolism provide cancer chemotherapy and antibacterial and antimalarial drugs because folic acid as tetrahydrofolate participates:
A. in the transamination and decarboxylation of amino acids.
B. as a one carbon group carrier in purine nucleotide de novo pathway.
C. in the interconversion of serine to glycine
D. in the methylation of dUMP to TMP.
(Answer: D / Reference A: p. 493, 494 / MPL 33)
89. Which vitamin and mineral is involved in blood coagulation?
A. Calcitriol and magnesium
C. Retinoic acid and potassium
B. Menaquinone and calcium
D. @ Tocopherol and sodium
(Answer: B / Reference A: p. 486, 600 / MPL 90)
90. Hyperhomocysteinemia, a risk factor for cardiovascular disease, is fairly common in the
elderly because of inadequate intake or under utilization of:
A. B1 and B3
C. B5 and B7
B. B2 and B5
D. B9 and B12
(Answer: D / Reference A: p. 492 / MPL 60)
91. Which of the following best describes the plasma proteins?
A. They are principally derived from the diet.
B. Almost all plasma proteins are simple proteins like albumin.
C. Their half-lives are unaltered by certain diseases.
D. Many plasma proteins exhibit polymorphism.
(Answer: D / Reference A: p. 582 / MPL 60)
92. Which of the following pathways is the source of fuel for the red blood cells?
A. oxidation of fatty acids
C. Transamination of glycine
B. Glycolysis of glucose
D. Ketone bodies degradation
(Answer: B / Reference A: p. 612 / MPL 90)
93. Which of the following neutrophilic enzymes catalyzes the formation of chlorinated oxidants
to kill bacteria?
A. Lysozyme
C. NADH oxidase
B. Myeloperoxidase
D. Superoxide dismutase
87. One of the following is INCORRECT as regards the importance of vitamin on the nervous
tissues:
A. Vitamin C acts as cofactor in the formation of catecholamines.
B. Thiamine triphosphate acts as phosphate donor for phosphorylation of the neural
membrane sodium transport channels.
C. Cobalamine acts as carrier of one-carbon group in the formation of gangliosides.
D. Pyridoxal phosphate plays a role in the synthesis of sphingolipids necessary for
myelin formation.
89. What is the type of hyperbilirubinemia seen in a 35 y.o. woman with sickle cell disease who
had hemolytic crisis?
A. Cholestatic jaundice
C. Kernicterus
B. Direct-reacting hyperbilirubinemia
D. Retention hyperbilirubinemia
90. The cause of jaundice in the above patient:
A. Decreased activity of hepatic UDP-glucuronosyl transferase
B. Decreased albumin binding to bilirubin
C. Decreased hepatic content of MOAT proteins.
D. None of the above
91. One of the laboratory findings revealed an increased urine urobilinogen. What is
responsible for this laboratory result?
A. Increased activity of the renal B glucuronidase activity.
B. The hydrophilic characterisitic of the direct-reacting bilirubin.
C. Increaed enterohepatic urobilinogen cycle
D. All of the above
92. Which of the following xenobiotic phase II conjugating substrate is also involved in
transport of certain amino acids into the cells?
A. Adenosine 3 phosho 5 phosphosulfate
B. Glutathione
C. S-adenosylmethionine
D. UDP-glucuronic acid
93. True of the cytochrome P450 systems, EXCEPT:
A. Catalyze reactions involving introduction of one atom of oxygen into the substrate
and one into water.
B. Exhibit broad substrates specificity
C. In some cases, their products are mutagenic and carcinogenic
D. All of the above
94. One of the following enzymes DOES NOT occupy a central position in amino acid
synthesis:
A. Amino oxidases
C. Glu dehydrogenase
B. Aminotransferases
D. Gln synthetase
95. One of the following best describes the biosynthesis of nutritionally nonessential amino
acids:
A. The coenzymes of biotin and cobalamin are involved in the pathways.
B. Nutritionally nonessential amino acids have short biosynthetic pathways.
C. Intermediates of glycogenlytic pathway serve as substrates.
D. Ammonia serves as the amido group for the formation of glutamine and
asparagine.
96. Which of the following amino acids can be synthesized from choline, glyoxylate or
serine?
A. Alanine
C. Glycine
B. Cysteine
D. Proline
97. Arginine is considered as a nutritionally semi-essential amino acid because:
A. it is derived from an essential amino acid.
B. it is synthesized at rates inadequate to support growth in children.
C. it is not commonly found in food.
D. Its intestinal transport system is not as efficient as the other amino acids.
98. Alcoholics are expected to develop any of the following, EXCEPT:
A. Fatty liver cirrhosis
C. Menkes syndrome
B. Hyperuricemia
D. Wernicke Korsakoffs syndrome
99. The biochemical reason for the increased deposition of triacylglycerol in hepatic tissues
of alcoholics:
A. Increased mobilization of fat from adipose tissues
B. Increased hydrolysis of lipoprotein triacylglycerol by lipoprotein lipase
C. Increased NADH/NAD ratio
D. A and C only
100. A metabolic block in the production of plasma VLDL resulting to fatty liver is seen in one
of the following conditions:
A. Alcoholism
C. Starvation
B. Chloroform poisoning
D. Uncontrolled diabetes mellitus
18. A common sugar in N-linked glycoproteins:
A. Fucose
B. Galactose
C. Glucose
D. Mannose
60. Which of the ff. is used to join the two molecules at the expense of high energy
phosphate bond of ATP?
A. Isomerases
C. Lyases
B. Ligases
D. Transferases
61. Characteristic feature of enzyme as a catalyst:
A. Raises the energy barrier of a reaction
B. Provides alternate transition state
C. Affects overall free-energy change for reaction
D. Involves transition states of high energy levels
62. Changes in the optimal pH value affect acidity of an enzyme by:
A. altering the conformation of catalytic site
B. a change in the allosteric site
SUBJECT
MATTER
QUESTION
NUMBERS