Académique Documents
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Procedure
Inoculate tubes of Litmus Milk with 18- to 24-hour pure
cultures. For the study of anaerobic organisms, sterile mineral
oil can be layered over the medium following inoculation. Incubate tubes at 35 2C for up to 14 days and record
reactions at various intervals during the incubation process.
Expected Results
Consult an appropriate reference for the expected reactions
for specific microbial species.2
References
Formula
BBL Litmus Milk
g
g
g
Availability
BBL Litmus Milk
Cat. No.
211343
221657
Dehydrated 500 g
Prepared Tubes Pkg. of 10*
*Store at 2-8C.
Formula
Difco Littman Oxgall Agar
Approximate Formula* Per Liter
Peptone ................................................................... 10.0
Dextrose ................................................................... 10.0
Oxgall ....................................................................... 15.0
Agar ......................................................................... 20.0
Crystal Violet .............................................................. 0.01
g
g
g
g
g
Saccharomyces cerevisiae
ATCC 9763
Identity Specifications
Difco Littman Oxgall Agar
Dehydrated Appearance:
Solution:
Prepared Appearance:
Reaction of 5.5%
Solution at 25C:
pH 7.0 0.2
Cultural Response
Difco Littman Oxgall Agar
Prepare the medium per label directions without (plain) and with the
addition of streptomycin. Inoculate and incubate at 25-30C for up to 72
hours.
ORGANISM
ATCC
INOCULUM RECOVERY
RECOVERY
CFU
PLAIN w/STREPTOMYCIN
Candida albicans
Escherichia coli
Saccharomyces cerevisiae
Saccharomyces cerevisiae
Trichophyton mentagrophytes
10231
25922
9763
9080
9533
102-103
Good
Good
102-103 Fair to good Inhibition
Good
Good
102-103
102-103
Good
Good
102-103 Fair to good Fair to good
Trichophyton
mentagrophytes
ATCC 28185
using one or more of the following techniques to corroborate cultural findings: direct microscopic examination of
the specimen; animal inoculation; biochemical determination; or serological procedures.
2. Do not use to culture Nocardia asteroides, Streptomyces
or any other microorganism sensitive to streptomycin.3
Expected Results
References
Procedure
Availability
Difco Littman Oxgall Agar
Cat. No.
229410
Dehydrated 500 g
311