Page 1 of 4

Effective Date: Oct 2003

Document Ref: PROD.P.007

PH ARM A LTD

STANDARD OPERATING PROCEDURE

FOR
OPERATION OF pHS-25 pH METER

DOCUMENT REFERENCE: PROD.P.007

Written by:

KELECHI .O. ATUONWU

OCTOBER 2007

Approved by: _______________________ Date ; ________

Review Period

3 years

Expiry Date

Oct 2010

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Date:

PH ARM A LTD

Page 2 of 4
Effective Date: Oct 2003
Document Ref: PROD.P.007

1. PURPOSE
For the safe and effective use and operation of the pHS -25 pH meter which is used to
measure the power of Hydrogen ion concentrations of any given aqueous solution in the
laboratory.

2. RESPONSIBILITIES
1. It is the responsibility of the QA Laboratory Analyst to ensure that the following
procedure is fully implemented.
2. It is the responsibility of the QA Laboratory Analyst to ensure that the equipment is
kept in the right operating standard always and that procedures are being followed.
3. It is the responsibility of QA Laboratory Analyst to ensure that this procedure is
followed strictly and any deviation reported immediately to the Q. A. Manager.

3. REFERENCES
pHS-25 pH meter operating manual.

4. DEFINITIONS
i

D.C. ; Direct current

ii.

A/D ; Analogue Digital.

5. PROCEDURE
OPERATIONAL REQUIREMENT
i.
ii.

iii.
iv.

Ambient Temperature;0 - 400 C

Relative Humidity;50 85%
Solution Temperature;5 - 60oC
Power Supply; 220v, 50Hz.

MAIN PARAMETERS
i.
ii.
iii
iv
v
vi

pH;0 14.00 pH(measuring range)

Range of temperature compensation; 0 - 60oC
Power consumption ;1W
Weight ;3 Kg
Input Impedance; 5 x 1011
Accuracy ; pH 0.1pH

PRINCIPLE OF OPERATION
The pH meter is based on a D.C. potential generated by a pH combination electrode.
The D.C. potential is fed to an A/D converter via a preamplifier for digital display of pH
values.
START UP CHECKS
I
Confirm correct connection of electrode terminal to the meter.
ii.
Confirm electrode is mounted on the electrode holder.
iii.
Confirm equipment is clean.
v.
Confirm equipment is placed on a vibration free workbench.

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PH ARM A LTD

Page 3 of 4
Effective Date: Oct 2003
Document Ref: PROD.P.007

START UP
STANDARDIZATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Note;

Switch on the equipment.

Select the selection knob to pH position.
Set the slope position to 100%.
Rinse the electrode with distilled water, and clean with tissue paper.
Put the electrode into the buffer solution of known pH (e.g. pH value 4.0)
Adjust the temperature regulator; stir the solution in the beaker until it is
uniform.
Adjust the setting potentiometer, to set the reading displayed on the meter
the same as the pH value of the buffer solution.
Record your readings in the calibration notebook.
Repeat steps 3 8 for buffer solutions of pH value 7.0, 10.0 respectively.
Once standardization is complete, its setting potentiometer should not be
changed anymore. The meter once standardized it could be used to measure
the pH of a solution at once.
during the time at which the meter is not in use, the bulb of the electrode
should be immersed in distilled water.

pH VALUE MEASUREMENT
(A)
i.
ii.
iii.
iv.
(B)
i.
ii.
iii.
iv.
v.
vi.

In case the temperature of the solution to be measured is the same as the

temperature of the setting solution.
Move the electrode clamp upward and remove it.
rinse the end of the electrode with distilled water and clean it with tissue
paper
Put the electrode into the solution to be measured, stir the solution till it is
uniform
Read out and record the pH value of the solution.
In case the temperature of the solution to be measured is different from the
temperature of the setting solution.
Move the electrode clamp upward and remove it.
Rinse the end of the electrode with distilled water and clean with a tissue
paper.
Measure the temperature of the solution to be measured with a thermometer.
Adjust the temperature regulator to the measured temperature value of the
solution.
Put the electrode into the solution to be measured, stir the solution until it is
uniform.
Read out and record the pH value of the solution.

RE-STANDARDIZATION
It is necessary to re standardize the meter in compliance with the standardization
procedure in case any of the following conditions occurs;
i.
The electrode has been dried for too long.
ii.
The electrode is a newly replaced one.
iii.
The setting potentiometer has changed.
iv.
After measuring an Acidic solution or Alkaline solution of high concentration.
v.
After measuring an organic solution of high concentration.
vi.
The temperature of the solution is largely different from the temperature at
the time of standardization.

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PH ARM A LTD

Page 4 of 4
Effective Date: Oct 2003
Document Ref: PROD.P.007

MAINTENANCE
i.
ii.
iii.

The meter should be cleaned daily.

The meter should be placed on a vibration free workbench.
The buffer solutions been used to standardize the pH meter should be of high
reliability.
Servicing and replacement of component parts should be done by certified
maintenance personnel.

iv.

6. HEALTH AND SAFETY

I. Wear proper safety wears- hand glove, Lab coat etc.
II. Ensure that connected terminals are functioning.
III. Dont put hand under electrode bulb.
IV. Power off the equipment before attempting any repair/cleaning on the
equipment.

7. CHANGE HISTORY
Nil.

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