KRS KKNI is under development

STATE UNIVERSITY OF MALANG

DEPARTMENT OF BIOLOGY
SYLLABI
Course Name

Biomolecular Analysis Techniques

Code

For Animal Studies

Lecturer Name

Dra. Dwi Listyorini Aljabari, M.Si, D.Sc

Semester

2th. Y 2014/2015

Credit/Hours

2/4

Student to attend

15 students/offering

Classroom

Bio 316

Prerequisites

Biochemistry, Cell Biology, Genetics I, Genetics II

CompulsoryElectiveOthers: Compulsory for Biology Program

CommonFundamentalSpecialOthers: Special
Course Objectives

1. To understand the basic works in molecular techniques.

2. To be able to perform certain basic techniques in molecular
works in a right way.
3. To be able to apply certain molecular techniques in a
proposed research project.
4. To be able to take a part in molecular laboratory jobs.

Course description

Introduction; mind setting; mental setting; basic works in

molecular techniques; gene anatomy; gene expression and
regulation; gene manipulation; molecular cloning; Recombinant
DNA; molecular techniques including: special equipment/devises
sterilization, micro pipetting, microtransfer, micro centrifuging,
temperature handling, temperature-sensitive reagents handling,
harmful reagent handling, whole DNA isolation, enzymatic DNA
digest, electrophoresis, gene to vector insertion, bacterial
transformation, molecular identification, PCR, DNA sequencing,
RNA probe synthesis. Bioinformatics: Genetics analysis for
identification, Biosystematics, Phyllogenetics and Regulation.

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KRS KKNI is under development

Compulsory
Textbooks

Brown, T. A. 2001. Genome. 2nd. Ed. NY: John Wiley & Sons,
Inc.
Freifelder, D. 1985. Essential of Molecular Biology. Boston:
Jones and Barlett Pub. Inc.
Listyorini, D., Winaris, N., & Kartikasari, N. 2009. Molecular
Handbook: A Practical Approach. Unpublished.
Sambrook, J., Fritsch, E.F. & Maniatis, T........ Molecular
Cloning: A Laboratory Manual. NY: Cold Spring Harbor
laboratory Press.
Stern, C. In Situ Hybridization.
Watson, J.D., Tooze, J. & Kurtz, D.T. 1983. Recombinant DNA:
A Short Course. NY: W.H. Freeman and Co.
Listyorini, D., Winaris, N., Kartikasari, N., Prananingrum, P., &
Anggorowati, D. 2012. Teknik Analisis Biologi Molekular.
Ed. 2. Unpublished.
Fatchiyah, dkk. 2012. Teknik Analisis Biologi Molekular
.................................. Barcoding untuk Fauna

Suggested
Reading

Recent research articles in the field of Cell Biology, Genetics,

Development, etc. published in the international journals.
Protocols from Biomolecular products.

Teaching
Material/ Aids

LCD projector & Computer; Laboratory equipments.

Teaching
Method

Theories for maximum 8 meeting

Evaluation
Method

Paper test

Lab works for maximum 8 meeting

Lab work
Individual report on lab work
Research Proposal

Request to
Student
(controlled
exercise,
experimental
investigation,
project, report)

To study and resume certain research reports published in

scientific international journals.
To attend 100% lab works (practice).
To present an individual report 24 hours after a lab work has
been completed.
To submit a research proposal in the end on semester

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KRS KKNI is under development

SCHEDULE
Week

Topic

Activities

Introduction: Theory on Basic

Techniques in Molecular
Works

Mind & Mental Setting; Basic Works in

Molecular Techniques; Basic Techniques
in Molecular Works

Washing & Sterilization &

Good Pipetting Practice

Lab Work: Equipment Washing &

Sterilization; Work Place Sterilization
Pipetting, Micropipetting, Microtransfer,
Micro Centrifuging

Theory on Gene Anatomy &

Regulation

Discussion: Gene Anatomy; Gene

Expression & Regulation

Simple DNA Isolation

Lab Work: Simple DNA Isolation (optional)

Molecular Genetics
Techniques for Taxonomic
and Regulation Studies

Discussion: DNA Isolation; Library DNA;

DNA storage and Management; Gene
Transfection, CISH; WISH; FISH; IHC;
Blottings; Tagging; Finger printing

Lab work: Plant Total DNA isolation

Discussion: DNA Quantitazion; Qualitative

Yield Examination, Electrophoresis:
Preparation, Reading and Presentation

Lab Work: DNA Quantization,

Electrophoresis Check

Discussion: Recombinant DNA, Bacterial

Transformation;

Lab Work: DNA Cutting, Electrophoresis

Check, DNA Ligation into Plasmid, Insert
Check, Bacterial Transformation

Discussion: Primer Designing Polymerize

Chain Reaction (PCR)

Lab Work: PCR and Sequencing

Discussion: Gene Manipulation and

6
7

Yield Examination & Trouble

Shooter

8
9

Molecular Cloning &

Recombinant DNA

10

11

Gene Isolation/Amplification

12
13

Gene Manipulation and

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KRS KKNI is under development

Genetic Analysis

14

Mutation, Bioinformatics, Gene Bank,

Phyllogenetic for Taxonomy and Evolution
Study, DNA Barcode

Lab Work: Sequence Examination,

Consensus Sequence, Open Reading
Frame (ORF), Genes Alignment,
Phyllogenetic Tree Reconstruction,
Genetic Distance Calculation and Analysis

15

Theory and Lab Work exam

Examination

16

Research Project Completion

Final Discussion on Proposed Research

Project

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