Code No: R05312304 Set No.

1
III B.Tech I Semester Supplimentary Examinations, February 2008
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. What is the sporulation signal in B.subtilis? How are the genes involved in sporu-
lation controlled? Explain in detail. [16]

2. What does gene amplification mean? How is it different from gene duplication?
[16]

3. How can you detect transposition in bacteria? Explain in detail the process of
transposition. [16]

4. How does one isolate the genomic DNA? What are the differences in DNA isolation
procedures in bacteria, plant cells and animal cells? [16]

5. How would you screen putative transformants for expression of the expected gene
product? [16]

6. What are the different variations of PCR? Explain in detail. [16]

7. What are the different types of Molecular markers used currently for diagno-
sis/identification purposes? [16]

8. Discuss the strategy that should be employed for cloning of therapeutically impor-
tant product like insulin. [16]

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Code No: R05312304 Set No. 2
III B.Tech I Semester Supplimentary Examinations, February 2008
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the following with examples:

(a) Activators
(b) Repressors
(c) Promoter
(d) Operator
(e) Cis acting
(f) Trans acting
(g) polycistronic
(h) monocistronic. [2 × 8]
2. Explain the following:
(a) Essential features of transcriptional activators
(b) Structural features of Transcription factor IIIA. [8 × 2]
3. How is chromosomal DNA separated from plasmid DNA during plasmid prepara-
tion? What is the function of EDTA in the TES buffer? Briefly describe the steps
involved in plasmid isolation procedures. [16]
4. How does one select a suitable vector for cloning and expression of a protein? [16]
5. Explain the dideoxy method in detail. [16]
6. Write short notes on:
(a) hot-start protocol
(b) nested primers
(c) Q-PCR
(d) real time QPCR. [16]
7. Write short notes on:
(a) oligo chips
(b) DNA arrays [8+8]
8. What do you understand by the terms Ex vivo and In vivo? Explain these in detail
with reference to gene therapy. [16]

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Code No: R05312304 Set No. 3
III B.Tech I Semester Supplimentary Examinations, February 2008
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the following with examples:

(a) Activators
(b) Repressors
(c) Promoter
(d) Operator
(e) Cis acting
(f) Trans acting
(g) polycistronic
(h) monocistronic. [2 × 8]
2. Explain the following:
(a) Essential features of transcriptional activators
(b) Structural features of Transcription factor IIIA. [8 × 2]
3. How do transposons differ from insertion elements? Explain in detail. [16]
4. Explain the construction of genomic libraries. Is it possible to get all the genes
cloned while constructing a genomic library? [16]
5. Explain the recent developments in DNA sequencing. [16]
6. How does one identify a PCR product? In a particular PCR amplification ex-
periment, the product obtained was shorter than the expected length. Give your
comments on the possible reasons. [16]
7. Write short notes on:
(a) oligo chips
(b) DNA arrays [8+8]
8. Write short notes on:
(a) recombinant insulin
(b) human growth hormone. [8+8]

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Code No: R05312304 Set No. 4
III B.Tech I Semester Supplimentary Examinations, February 2008
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. How do you define an operon? Explain the Control of gene expression in bacteria
by citing the example of any operon. [16]

2. Explain the following terms:

(a) Regulatory sequences

(b) Enhancers
(c) reporter genes
(d) gene silencing. [4 × 4]

3. How is chromosomal DNA separated from plasmid DNA during plasmid prepara-
tion? What is the function of EDTA in the TES buffer? Briefly describe the steps
involved in plasmid isolation procedures. [16]

4. What are the different types of cloning vectors used in genetic engineering? [16]

5. Explain the recent developments in DNA sequencing. [16]

6. Comment on nature of enzymes used in PCR along with their importance. [16]

7. How are the DNA arrays produced? Discuss some of the technologies available for
the production. [16]

8. Discuss the strategy that should be employed for cloning of therapeutically impor-
tant product like insulin. [16]

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