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(INDEPENDENT
VARIABLE)
Amount
5 different
concentrations
Different
dilutions will be
made.
Approximately
20ml ( to be split
later for
Test tubes
Pipettes
dilutions)
6
6
Stopwatch
(DEPENDENT VARIABLE)
Beakers
To hold experiment
To accurately add any solutions i.e.
casein.
This will be placed behind each test
tube- this is to ensure maximum
accuracy f our results. Not only will we
have the colour change as an
indicator but The point at which the
cross can be see is also an indication
the reaction has finished
Risks
This is a relatively low risk experiment, however to ensure there are one or two
precautions we must take. Firstly, as trypsin is a digestive enzyme, it is worth
preventing spillages as if this were to come in contact with the eyes, it could be
very damaging. For this reason we can wear eye protection. Also, if spillages do
occur wearing an apron to prevent clothes being damaged, is one way to reduce
this risk. Moreover, we must be careful when handling glassware in this
experiment and make sure test tubes are left in their test tube holder to ensure
they do not roll of the table etc.
Method:
1) Prepare the equipment needed for the experiment (given above)
2) Prepare 6 concentrations on enzyme solutions.
The following dilutions should be prepared:
0.8
0.6
0.4
0.2
Results Table
Mean Time in
Seconds
0.2
1225
1471
984 (TOO
LOW)
1348
0.4
723
872
563 (TOO
LOW)
797.5
0.6
364
512
385
420.3
0.8
225
258
320
267.6
209
239
154
200.6
Anomalies are written in blue, I deemed these as anomalous readings as they did
not quite fit the pattern of results therefore, to ensure accuracy of results I didnt
include these values in the average times of each concentration.
NB:the first value I feel should be somewhere within the 1220-1550 bracket
the second value should be higher, within the 600-900 bracket
Evaluation of Conclusion
Evaluation of method:
Overall I feel my method was very reliable I used certain utensils for example,
pipettes to ensure accuracy of the experiment. Moreover, it was easy to follow
with simple steps. There was however many flaws in the experiment. I feel as
though we should have done a netter job at controlling other variables. We only
left the test tubes to react in a room where temperatures may vary at different
times. Temperature should be kept constant for this reason if I were to do this
experiment again I would leave the test tubes in a after bath at 35 degrees as
this is the optimum temperature not just for trypsin but many other enzymes in
the body. Moreover, there was room for human error when pressing the
stopwatch as someones perception of white to colourless may be different to
someone elses therefore the time may not be as accurate. Therefore, next time
we should have only one person in charge of the stopwatch.
Overall, to take this investigation further, I would have liked to taken the results
of even more concentrations of trypsin to be more precise i.e 2%,3% and so on,
to provide an even more valid conclusion or I would have looked at other factors
such as the effect on temperature. I probably would have wanted to take this
investigation further until substrate concentration could increase no more and
the solution became saturated
I would have probably tested more enzymes such as pepsin another digestive
enzyme to see whether the effects are similar and compare my results.